Life Sciences Commons^™

Creating Sustainable School And Home Gardens: Organic Pest Management, Ruby Mohr, Kathy Cabe Trundle, Rita Hagevik

All Current Publications

This fact sheet on organic pest management outlines sustainable gardening practices that promote ecological balance and biodiversity. It defines organic pest management as a proactive method, using natural insecticides like sulfur and neem oil, along with unique pest control strategies such as beer traps and cinnamon repellents. Additionally, it highlights plants like mint and marigolds that repel harmful pests while attracting beneficial insects. By implementing these approaches, gardeners can protect crops sustainably, enhance soil health, and reduce reliance on synthetic pesticides, thus supporting a healthier food system.

A Role For The Actin Cytoskeleton And Plastin-3 In Osteoblast Mineralization And Mechanosensation, Samantha Meagan Chin

Arts & Sciences Graduate Student Theses and Dissertations

The great diversity in actin network architectures and dynamics is exploited by cells to drive fundamental biological processes including cell migration, intracellular trafficking, and cell division. One such system that relies on the actin cytoskeleton to fulfill its physiological function are osteoblasts, which are mesenchymal lineage bone cells that promote bone formation through depositing a collagen scaffold and subsequently promoting mineralization of this matrix. A number of factors are known to drive osteogenic differentiation of pre-osteoblast cells including extracellular matrix cues such as substrate stiffness, which are primarily detected via mechanosensitive machinery linked to the actin cytoskeleton. Disruptions to β-actin, …

Assessment Of Fish Biodiversity Of The Cimandiri Watershed, West Java, Indonesia, Rudhy Gustiano, Haryono Haryono, Gema Wahyudewantoro, Krismono Krismono, Rusdianto Rusdianto, Firman M. Nur

Makara Journal of Science

Information on the fish biodiversity of the Cimandiri Watershed is incomplete, and human activities and anthropogen-ic impacts pose a significant threat to this biodiversity. The purpose of this study was to assess the biodiversity of fish in the Cimandiri Watershed. The results indicated that fish biodiversity in the area included 104 species belonging to 49 families. There were 26 species in the upstream area, 47 species in the middle area, and 61 species in the downstream area. Forty-three species were native to Indonesia, and 7 of the native fish were endemic to the area. There were 11 introduced species belonging …

Diversity And Distribution Of Macrofungi In The Mid-Hills Of Bagmati Province, Central Nepal, Hari Sharan Adhikari, Suman Shrestha, Manoj Gurung, Sushma Pandit, Sujan Balami

Makara Journal of Science

The current study was carried out at seven research sites in the mid-hills (900-2500m asl) across six districts in Bagmati province, central Nepal to analyze the variety and distribution of macrofungal communities. The survey was carried out in the wet seasons from August 2017 to September 2022, including frequent field visits to the selected forests. Vari-ous diversity indices were applied to ascertain the composition of the macrofungal communities in the study area. A total of 133 wild macrofungal species belonging to 80 genera, 50 families, and 19 orders were observed. The macro-fungal population in the Bagmati province was dominated by …

Development Of A Multi-Epitope Peptide Vaccine Against Monkeypox Virus: Immunoinformatics Analysis For South East Asian Hla Alleles, Nelson Chandra, Mochammad Aqilah Herdiansyah, Viol Dhea Kharisma, Arif Nur Muhammad Ansori, Arli Aditya Parikesit

Makara Journal of Science

The monkeypox virus (MPXV), a DNA virus causing zoonotic disease, poses major global public health challenges, with mortality rates between 3%–6%. Although smallpox vaccines provide partial cross-protection, there is a critical need for a dedicated, effective monkeypox (mpox) vaccine. This study aimed to design a multi-epitope peptide-based vaccine specifically adapted to the HLA allele profiles common in Southeast Asian populations, where MPXV cases are rising. Using immunoinformatics, we screened for and detected B and T cell epitopes from the MPXV cell surface antigen and IFN-alpha/beta receptor proteins. The vaccine design was validated through a rigorous evaluation of its antigenicity, immunogenicity, …

Cloning And Expression Of Scamp3 In Escherichia Coli Bl21(De3) With In Silico Sequence-Based Cancer Epitopes Prediction, Selly Setiati Rajagukguk, Sabar Pambudi, Astari Dwiranti, Doddy Irawan Setyo Utomo, Anom Bowolaksono

Makara Journal of Science

Secretory carrier membrane protein 3 (SCAMP3) is a crucial membrane protein involved in intracellular vesicle traffick-ing and exocytosis. The SCAMP3 expression has been observed in diverse cancer types, such as melanoma, glioma, hepatocellular and breast cancer. Increased SCAMP3 expression has been reported in certain cancer cells relative to that in normal cells, suggesting the potential role of SCAMP3 in cancer development or progression. In this study, we successfully cloned and expressed SCAMP3 in Escherichia coli strain BL21(DE3). SCAMP3 was amplified and insert-ed directionally into the prokaryotic expression vector pET21d(+). The transformation of recombinant plasmid into E. coli BL21(DE3) cells were …

Screening For Penicillin G Acylase (Pga)-Producing Bacteria And Gene Cloning Using Degenerate Oligonucleotide Primed-Pcr, Masdalifah Masdalifah, Sri Rezeki Wulandari, Gabriela Christy Sabbathini, Maria Ulfah, Dini Achnafani, Ahmad Wibisana, Feronika Heppy Sriherfyna, Is Helianti, Niknik Nurhayati

Makara Journal of Science

The growing concern over antibiotic resistance has driven global efforts to explore innovative solutions, including the use of Penicillin G acylase (PGA) to produce semisynthetic β-lactam antibiotics. This study screened four potential in-tracellular PGA-producing bacteria: Alcaligenes faecalis InaCC B444 (AfPGA), Kluyvera cryocrescens InaCC B850 (KcPGA), Providencia rettgeri InaCC B25 (Pr25PGA), and P. rettgeri InaCC B466 (Pr466PGA). Penicillin G Acylase encoding genes (pgas) were isolated from them using a Degenerate Oligonucleotide Primed-PCR (DOP-PCR) approach and sequenced. Microbiological assays confirmed all tested crude extracts to exhibit inhibitory effects. Penicillin G was used for evaluating hydrolytic activity and 6-Amino Penicillanic Acid (6-APA) coupled …

Molecular Interaction Of Purple Rice Ferulic Acid And Skin Aging-Related Enzymes, Ernanin Dyah Wijayanti, Fatchiyah Fatchiyah, Anna Safitri, Dian Siswanto

Makara Journal of Science

Purple rice is rich in ferulic acid, a phenolic acid abundant in rice. Ferulic acid (FA) has long been recognized for its anti-oxidant properties, which are linked to its skin anti-aging activity owing to its ability to scavenge free radicals and pro-tect cells from damage. Skin anti-aging activity could be assessed by inhibiting skin aging enzymes. However, there was limited information on the mechanism of FA inhibition of these enzymes. This study focused on the molecular interac-tion between purple rice FA and collagenase or tyrosinase, both with and without their substrates. The interaction be-tween FA (CID 445858), collagenase (2Y6I), tyrosinase …

Roles Of The Survivin Bir Domain In Cellular Apoptosis And Proliferation: An In Silico Study, Silviatun Nihayah, Septelia Inawati Wanandi, Linda Erlina, Resda Akhra Syahrani

Makara Journal of Science

Survivin is an antiapoptotic protein that is highly expressed in cancer cells. We investigated the dual roles of the Bacu-lovirus IAP Repeats (BIR) domain within survivin, encompassing both apoptosis and proliferation, through an in silico study. The protein-protein interaction (PPI) network of survivin was analyzed using Cytoscape software. Functional enrichment (FE) analysis and data from the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to identify the implicated signaling pathways. The binding affinity of the BIR domain with the targeted proteins was visualized via molecular docking analysis. Drawing insights from the PPI network and FE analysis, we identified two …

Antibacterial Activity Of Endophytic Actinobacteria Isolated From Ziziphus Mauritiana Against Multidrug-Resistant Strains, Hafifatunil Khairani, Jepri Agung Priyanto, Muhammad Eka Prastya, Dwi Retnowati

Makara Journal of Science

Increasing resistance to existing antibiotics has led to a need for new alternative sources. Among the possibilities that need to be explored is the endophytic actinobacteria associated with Ziziphus mauritiana. The present study aimed to evaluate the antibacterial activity of endophytic actinobacteria isolated from Z. mauritiana against five multidrug-resistant bacterial strains. Thirty actinobacteria isolates were obtained from the roots, stem, and leaves of the plant using the serial dilution technique. Using a double-layer agar test, we found that colonies from five of the 30 isolates (16%) exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and the M18 strain of Bacillus …

A Phase I/Ii Trial Of Avelumab Combinations With Ivuxolimab, Utomilumab, And Radiation Therapy In Patients With Advanced Gastrointestinal Malignancies, Jibran Ahmed, Anne Knisely, Carlos Torrado, Bettzy Stephen, Yali Yang, Juhee Song, Anas Alshawa, Abdulrazzak Zarifa, Anuja Jhingran, Eugene J Koay, Van Karlyle Morris, Milind Javle, Robert A Wolff, Funda Meric-Bernstam, Shubham Pant, Jordi Rodon, Aung Naing

Faculty, Staff and Student Publications

Background: Checkpoint agonists utomilumab (4-1BB agonist) and ivuxolimab (OX40 agonist) enhance Teffector cell function. Preclinical studies suggest that combining these drugs with avelumab (anti-PD-L1 antibody) can potentially synergize this effect. In addition, tissue abscopal effects of radiation therapy may improve antigen presentation, complementing PD-L1 blockade. We conducted a single institution, open-label, multi-arm, non-randomized, phase 1/2 clinical trial of avelumab in combination with ivuxolimab, with or without utomilumab, and radiation therapy in patients with advanced solid tumors. Herein, we present a subgroup analysis in patients with gastrointestinal (GI) tumors (pancreatic, colon, gastric, and hepatocellular).

Methods: The primary objectives of this study …

Advances In Extracellular Matrix-Associated Diagnostics And Therapeutics, Morten Karsdal, Thomas R Cox, Amelia L Parker, Nicholas Willumsen, Jannie Marie Bülow Sand, Gisli Jenkins, Henrik H Hansen, Anouk Oldenburger, Kerstin E Geillinger-Kaestle, Anna Thorsø Larsen, Darcey Black, Federica Genovese, Alexander Eckersley, Andrea Heinz, Alexander Nyström, Signe Holm Nielsen, Lucas Bennink, Lars Johannsson, Anne-Christine Bay-Jensen, Dana E Orange, Scott Friedman, Mads Røpke, Vincent Fiore, Detlef Schuppan, Florian Rieder, Benjamin Simona, Lee Borthwick, Mark Skarsfeldt, Haakan Wennbo, Paresh Thakker, Ruedi Stoffel, Graham W Clarke, Raghu Kalluri, Darren Ruane, Faiez Zannad, Joachim Høg Mortensen, Dovile Sinkeviciute, Fred Sundberg, Molly Coseno, Christian Thudium, Adam P Croft, Dinesh Khanna, Michael Cooreman, Andre Broermann, Diana Julie Leeming, Ali Mobasheri, Sylvie Ricard-Blum

Faculty, Staff and Student Publications

The extracellular matrix (ECM) is the common denominator of more than 50 chronic diseases. Some of these chronic pathologies lead to enhanced tissue formation and deposition, whereas others are associated with increased tissue degradation, and some exhibit a combination of both, leading to severe tissue alterations. To develop effective therapies for diseases affecting the lung, liver, kidney, skin, intestine, musculoskeletal system, heart, and solid tumors, we need to modulate the ECM's composition to restore its organization and function. Across diverse organ diseases, there are common denominators and distinguishing factors in this fibroinflammatory axis, which may be used to foster new …

Incidence And Correlates Of High-Grade Chemotherapy-Induced Peripheral Neuropathy In Patients With Lung Cancer, Mitchell S Von Itzstein, Sawsan Rashdan, Suzanne E Dahlberg, David E Gerber, Alan B Sandler, Joan H Schiller, David H Johnson, Yating Wang, Zhuoxin Sun, Suresh S Ramalingam

Faculty, Staff and Student Publications

Background: High-grade chemotherapy-induced peripheral neuropathy (CIPN) represents a dreaded toxicity of cancer treatments. In some cases, it may limit activities of daily living and become permanent. Because many prior studies of CIPN were conducted in breast cancer populations, less is known about CIPN in men. We therefore determined the incidence and correlates of high-grade CIPN in a large cohort of patients with lung cancer.

Methods: We collected data from the ECOG-ACRIN E1594 (comparison of 4 chemotherapy regimens: cisplatin-paclitaxel, cisplatin-gemcitabine, cisplatin-docetaxel, carboplatin-paclitaxel) and E4599 (carboplatin-paclitaxel ± concurrent and maintenance bevacizumab) clinical trials. We identified cases with grade ≥3 CIPN. Multivariable logistic …

Prophylaxis, Clinical Management, And Monitoring Of Datopotamab Deruxtecan-Associated Oral Mucositis/Stomatitis, Funda Meric-Bernstam, Aditya Bardia, Paolo Bossi, Giampaolo Bianchini, Frances Gatlin, Rajesh V Lalla, Barbara Melosky, Naoki Niikura, Timothy A Yap, Sophie S Kim, Rachana Rajagopalan, Rick M Fairhurst, Stephanie L Graff, Hope S Rugo

Faculty, Staff and Student Publications

Oral mucositis/stomatitis (hereafter stomatitis) is a common dose-limiting toxicity seen with various classes of cancer treatment. Symptoms associated with stomatitis, primarily oral pain, may impact patient quality of life and may lead to dose delay and reduction or treatment discontinuation. Datopotamab deruxtecan (Dato-DXd) is a novel trophoblast cell surface antigen 2-directed antibody-drug conjugate undergoing clinical investigation in multiple solid tumor types. Stomatitis is among the most reported adverse events associated with Dato-DXd, with most cases being grades 1-2. This article reviews the incidence of stomatitis seen with Dato-DXd, including in the phase III pivotal studies TROPION-Lung01 and TROPION-Breast01 (in patients …

Curcumin Attenuates Smoking And Drinking Activated Nf-Κb/Il-6 Inflammatory Signaling Axis In Cervical Cancer, Vivek K. Kashyap, Prashanth Kb Nagesh, Ajay K. Singh, Andrew Massey, Godwin P. Darkwah, Aaron George, Sheema Khan, Nadeem Zafar, Santosh Kumar, Namita Sinha, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Background: High-risk strains of HPV are known to cause cervical cancer. Multiple clinical studies have emphasized that smoking and drinking are critical risk factors for cervical cancer and its high-grade precursors. In this study, we investigated if smoking and/or drinking augment the molecular mechanisms of the cervical carcinogenesis and defined a potential therapeutic approach for their attenuation.

Methods: The impact of benzo[a]pyrene (B[a]P) and/or ethanol (EtOH) exposure on cervical cancer cells was assessed by measuring changes in their cell migration and invasion characteristics. Expression of HPV16 E6/E7, NF-κB, cytokines, and inflammation mediators was determined using qRT-PCR, immunoblotting, ELISA, luciferase reporter …

Targeted Nanomedicine For The Modulation Of Pancreatic Tumor Immune Surveillance, Vivek Kumar Kashyap, Neeraj Chauhan, Mohammed Sikander, Eswara N. H. K. Ghali, Rahul Tiwari, Shabnam Malik, Sheema Khan, Bilal B. Hafeez, Murali M. Yallapu, Subhash C. Chauhan

Research Symposium

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal types of cancer, characterized by a dismal 5-year overall survival rate, primarily due to the lack of early detection and limited effective treatment options available. Gemcitabine (GEM) is the most effective therapy for PanCa, but it only offers a marginal survival benefit of 6 months. A combination of Paclitaxel (PTX) albumin nano-formulation (Abraxane) and Gemcitabine has shown marginal improvement in pancreatic cancer treatment. This poor treatment response has been associated with desmoplastic stroma, microbiome effects, altered tumor microenvironment (TME), suppressive immune cells and PDAC-specific signaling pathways. Our research has demonstrated …

Dark Chocolate Elevates Resting Energy Expenditure In Postmenopausal Women, Aubrey L. Johnson, Michael Webster

International Journal of Exercise Science

International Journal of Exercise Science 18(6): 316-328, 2025. Several recent reports have indicated positive health benefits of consuming (-)-epicatechin-rich cocoa products. Postmenopausal women are predisposed to reduced metabolism due to decreased levels and activity of the sex hormones estrogen, progesterone, and estradiol. The purpose of this study was to investigate the influence of dark chocolate consumption on resting and exercise metabolism in postmenopausal women. Using a randomized, double-blind design, 26 postmenopausal participants were assigned to a 30-day supplementation with 20-g per day of 72% dark chocolate (DC) or calorically matched white chocolate (WC). Before supplementation, participants underwent two control trials …

Meeting Carbon Dioxide Removal Demand In 2030: The Potential Of Macroalgae Cultivation And Harvest, Lotta Siebert, Jiajun Wu, Lena-Katharina Bednarz, David Keller, Felix Meier, Christine Merk, Sonja Peterson, Wilfried Rickels

Journal of Ocean and Coastal Economics

A growing number of countries have announced net-zero and net-negative emissions targets, but only a few countries provide incentives for carbon dioxide removal (CDR). We derive estimates of countries' hypothetical demand for CDR in 2030 based on their emissions reduction targets under the Paris Agreement. The aggregated average global demand for CDR in the compliance year 2030 is 1064 MtCO₂, 353 MtCO₂, and 124 MtCO₂ for the low, medium, and high-cost CDR scenarios, respectively. This demand comes exclusively from countries and regions with relatively high GDP per capita, relatively high abatement costs and a limited …

Multi-Ancestry Transcriptome-Wide Association Studies Of Cognitive Function, White Matter Hyperintensity, And Alzheimer's Disease, Dima L Chaar, Zheng Li, Lulu Shang, Scott M Ratliff, Thomas H Mosley, Sharon L R Kardia, Wei Zhao, Xiang Zhou, Jennifer A Smith

Faculty, Staff and Student Publications

Genetic variants increase the risk of neurocognitive disorders in later life, including vascular dementia (VaD) and Alzheimer's disease (AD), but the precise relationships between genetic risk factors and underlying disease etiologies are not well understood. Transcriptome-wide association studies (TWASs) can be leveraged to better characterize the genes and biological pathways underlying genetic influences on disease. To date, almost all existing TWASs on VaD and AD have been conducted using expression studies from individuals of a single genetic ancestry, primarily European. Using the joint likelihood-based inference framework in Multi-ancEstry TRanscriptOme-wide analysis (METRO), we leveraged gene expression data from European ancestry (EA) …

Comparative Evaluation Of Bacterial Adhesion And Biofilm Formation On Contact Lenses, Lina M. Shaker, Wan Nor Roslam Wan Isahak

AUIQ Complementary Biological System

This study evaluates bacterial adhesion, biofilm formation, and Staphylococcus aureus (S. aureus) (ATCC 25923) colonization on medical and cosmetic contact lenses made from Hilafilcon B and Omafilcon B under simulated ocular conditions. Non-incubated lenses served as controls, and experiments were repeated three times with a variability margin of ±5%, ensuring accuracy and reliability. Results revealed that biofilm growth and bacterial adhesion were influenced by lens material, type, and incubation duration. Cosmetic lenses made from Hilafilcon B exhibited the highest biofilm growth (1.95%) after one day, highlighting their susceptibility to microbial colonization. Conversely, medical lenses made from Hilafilcon B …

Removal Of Cr(Vi) From Aqueous Solution Using Magnetic Activated Carbon Prepared From Waste Shell Of Marigold Flower Straw: Kinetics, Isotherm And Mechanism Studies, Shanmukha Rao Metta, Uttam Kumar Sahu

AUIQ Complementary Biological System

When Cr(VI) was released from industrial waste into water bodies harms aquatic ecosystems and poses risks to human well-being. To reduce risks, it's crucial to lower Cr(VI) levels through the sustainable process. In order to extract Cr(VI) from a liquid, the magnetic activated carbon adsorbent was created for the current investigation. H₃PO₄ was utilised as an activating agent in the thermal procedure to prepare activated carbon from marigold flower straw for the first time. The marigold flower straw activated carbon (MFSAC) was further decorated with Fe₃O₄ nanoparticles (Fe₃O₄/MFSAC) in the …

Molecular Model Of Tfiih Recruitment To The Transcription-Coupled Repair Machinery, Tanmoy Paul, Chunli Yan, Jina Yu, Susan E Tsutakawa, John A Tainer, Dong Wang, Ivaylo Ivanov

Faculty, Staff and Student Publications

Transcription-coupled repair (TCR) is a vital nucleotide excision repair sub-pathway that removes DNA lesions from actively transcribed DNA strands. Binding of CSB to lesion-stalled RNA Polymerase II (Pol II) initiates TCR by triggering the recruitment of downstream repair factors. Yet it remains unknown how transcription factor IIH (TFIIH) is recruited to the intact TCR complex. Combining existing structural data with AlphaFold predictions, we build an integrative model of the initial TFIIH-bound TCR complex. We show how TFIIH can be first recruited in an open repair-inhibited conformation, which requires subsequent CAK module removal and conformational closure to process damaged DNA. In …

External Jugular Vein Catheterization Technique For Continuous Blood Sampling In Gilts, Preechaphon Taechamaeteekul, Yosua Kristian Adi, Chutikan Srisang, Pirada Virankabutra, Natchanon Dumniem, Jinda Singlor, Padet Tummaruk

The Thai Journal of Veterinary Medicine

External jugular vein blood collection is common in gilts and sows, but continuous sampling for hormonal and pharmacokinetic research requires catheterization. The standard protocol has remained largely unchanged for nearly 40 years. This study aimed to develop a modified external jugular vein catheterization technique in gilts. Ten gilts (72.0 ± 3.4 kg) underwent surgery under general anesthesia after 12-hour fasting. Sedation was induced with intramuscular Xylazine (4.4 mg/kg) and Tiletamine–Zolazepam (2.2 mg/kg), followed by intravenous Thiopental sodium (10 mg/kg). Once anesthetized, gilts were placed in dorsal recumbency, and the external jugular fossa was prepared under sterile conditions. A 10 cm …

Dysregulation Of Cholesterol Metabolism And Sorafenib Drug Resistance In Hepatocellular Carcinoma, Veerababu Nagati, Dennis Kwabiah, Yamile Abuchard Anaya, Ana Ayala Pazzi, Manish K. Tripathi

Research Symposium

Background: Hepatocellular carcinoma (HCC) is among the most prevalent cancers and a leading cause of cancer-related deaths worldwide. Sorafenib, a multikinase inhibitor, serves as a key first-line treatment for HCC when surgical intervention is not an option. However, primary and acquired resistance to sorafenib has significantly limited its effectiveness, reducing the disease control rate. Dysregulation of numerous genes that influence cancer cell proliferation, survival, and drug resistance contributes to this challenge. Identifying the molecular drivers of sorafenib resistance remains critical to improving treatment outcomes. Several factors, including activation of oncogenic pathways (e.g., Akt/mTOR), hypoxia, cholesterol metabolism, EMT, multidrug resistance, and …

Virulence Analysis, Characterization Of Integron, And Antimicrobial Resistance Genes In Strains Of Acinetobacter Baumannii Recovered From The Hydrological Sub-Region Of Soto La Marina, Tamaulipas, Tunde Olarinde Olaniyan, Ana Verónica Martínez Vázquez, Susana Silvia Fernández Dávila, Cesar Marcial Escobedo Bonilla, Virgilio Bocanegra Garcia

Research Symposium

Background: Acinetobacter baumannii (A. baumannii) is an important bacterium due to its frequent recovery as a multidrug-resistant pathogen in hospital settings. Its antimicrobial resistance capacities made it an important challenge to health care. Colistin has been adopted as the last resort drug for managing infections associated with A. baumannii and many other multidrug-resistant Gram-negative bacteria (MDR-GNB). Because of the rise in the use of colistin for the treatment of MDR-GNB, the evolution and spread of colistin-resistant bacteria is possible thus raising a potential threat to public health. However, we have chosen to focus on A. baumannii, a …

Investigating The Ability Of Foxo1 To Maintain Stem Cells, Stella A. Rios, Megan E. Keniry

Research Symposium

Background: Our group discovered that FOXO transcription factors drive stem gene expression in cancer to promote aggressiveness. Work by our group and others demonstrated that FOXO factors universally maintain stem cells including in cancer, embryonic, hematogenic and neuronal contents. Our current efforts are delineating the molecular underpinnings by which FOXO factors halt differentiation to maintain stem cells.

In this project, we are employing myoblasts as a valuable model to examine how Foxo1 maintains stem cells. Foxo1-regulated genes in myoblasts closely parallel its targets in glioblastoma and basal breast cancer stem cells, including targets such as Leukemia Inhibitory Factor, …

Studying The Significance Of Muc13 In Hepatocellular Carcinoma, Shabnam Malik, Mohammed Sikander, Daniel Zubieta, Parvez Khan, Murali M. Yallapu, Deepshikha P. Katare, S.K Jain, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Background: Hepatocellular carcinoma (HCC) is associated with a poor prognosis due to limited therapeutic options and a lack of early diagnostic markers. Recent studies have highlighted the elevated expression of mucin 13 as a potential oncogene and predictive biomarker in various cancers. However, its role in the development and progression of HCC remains largely unexplored. This study aimed to investigate the expression of mucin 13 in a chemically induced model of hepatocellular carcinoma.

Methodology: We employed a chemically induced model of hepatocellular carcinoma in Male Wistar rats using diethyl nitrosamine and 2-acetylaminofluorene. Serum and tissue samples were collected at …

Glucose Metabolism And Ldha Expression Are Modulated By Lncrna In Colorectal Cancer., Salique Hassan Shaham, Ricardo Pequeno Bracho, Manish Kumar Tripathi

Research Symposium

Abstract: Colorectal cancer (CRC) ranks as the third most commonly diagnosed malignancy globally and is the second leading cause of cancer-related deaths in the United States. In 2024, an estimated 152,810 new cases of CRC are projected, with 53,010 fatalities. The prognosis for metastatic CRC (mCRC) remains grim, with a survival rate of less than 15%. Urothelial cancer-associated 1 (UCA1), a long noncoding RNA (lncRNA), is dysregulated in CRC and has been implicated in tumor progression. Our research highlights significantly elevated UCA1 expression in metastatic CRC cells, where its association with the GLUT1 and the metabolic enzyme lactate dehydrogenase A …

Uca1 As A Key Regulator Of The Warburg Effect During Anoikis Resistance In Colorectal Cancer Metastasis, Ricardo Pequeno Bracho, Salique Hassan Shaham, Yamile Abuchard Anaya, Sophia Leslie, Kyle Doxtater, Subhash Chauhan, Bilal Hafeez, Tamer Oraby, Manish K. Tripathi

Research Symposium

Colorectal carcinoma (CRC) is the second leading cause of cancer-related mortality in the United States. While localized CRC has a 90% five-year survival rate, this drops sharply to 14% upon metastasis. Metastasis occurs in approximately 40–50% of CRC cases and requires cancer cells to acquire anoikis resistance—a critical adaptation allowing survival after detachment from the extracellular matrix, enabling migration and colonization of secondary sites. Understanding the molecular mechanisms driving anoikis resistance, particularly those linked to altered glucose metabolism, is essential for developing targeted therapies for metastatic CRC.

Cancer cells frequently exhibit the Warburg Effect, a metabolic adaptation favoring glycolysis over …

Restricted Feeding Of Goats During The Last Third Of Gestation And Trans-Generational Effects On Plasma Progesterone In Their Female Offspring, Andrew A. Ponter, Bérengère Laporte, Sabine Roussel, Winai Kaewlamun, Pascale Chavatte-Palmer, Christine Duvaux-Ponter

The Thai Journal of Veterinary Medicine

Feeding levels can vary in goat farms for several reasons. Underfeeding of gestating dairy goats can occur and may influence fetal follicle development and future reproductive performance in their offspring. The objective of this experiment was to study the effect of restricted feeding during the last third of gestation on some reproductive parameters in female offspring (F1). Two feeding groups were formed using 60 Alpine and Saanen dairy goats to produce the F1 female offspring. The control group (C, n = 30) was fed to requirements. The restricted group (R, n = 30) was given the same diet, but the …