Life Sciences Commons^™

Simulating Chill: Exploring The Cognitive And Therapeutic Potential Of Cold Vr Environments, Jessica Turner, Piper Hutson, James Hutson

Faculty Scholarship

This study investigates the cognitive and therapeutic potential of immersive virtual reality (VR) environments designed to simulate cold conditions. Through the engagement of participants through multisensory stimuli—including vivid visual representations of the Athabasca Glacier, auditory effects of icy winds, and corresponding haptic feedback—the research evaluates neurological and physiological responses associated with attention, emotional regulation, and stress modulation. Participants experienced virtual scenarios featuring icy winds and snow, activating specific neurological pathways involving the occipital lobe, primary visual cortex, superior colliculus, and insula, thus reinforcing sensory integration. Through predictive coding, the anterior insula and hypothalamus were engaged, prompting thermoregulatory simulations and subconscious …

Leveraging Natural Variation In Yeast To Understand Susceptibility Differences In Parkinson’S Disease, Julio Andres Molina Pineda

Graduate Theses and Dissertations

Parkinson’s Disease (PD) is a neurodegenerative disorder that causes countless suffering around the world. Both forms, familial and sporadic, have been associated with the misfolding and cytotoxicity of α-synuclein, an otherwise non-pathogenic protein suspected to have various roles in the normal maintenance of the central nervous system. Model organisms have emerged as great vessels to uncover the cellular and molecular mechanisms that shape α-syn biology, and great strides have been made to understand it. Yet, it is still unclear why some individuals are affected by α-syn toxicity, while others not so much. Since it’s extremely complicated to study and probe …

Identification Of New Germplasm Sources And Traits For Breeding Heat-Tolerant Soybean Varieties, Shelby Hammond

All Theses

The heat-tolerant soybean breeding is constrained by limited genetic diversity for heat tolerance, lack of efficient selection criteria, and incomplete knowledge of heat tolerance mechanisms. The objectives of this study were to characterize the heat tolerance of a soybean recombinant inbred line (RIL) population based on traits defining leaf function, pollen viability and yield, and identify genotypes and traits that can be included in breeding programs for heat tolerance. Field trials were conducted at Pendleton, SC in 2022 and 2023 to test the heat stress responses of 192 RILs [derived from DS 25-1 (heat tolerant) x DT97-4290 (heat-susceptible)], parental lines, …

Characterizing Xylella Fastidiosa Pathogen Dynamics On Diverse Small Fruit Farms, Alexandra Ratay

All Theses

Global agriculture has been threatened for over a century due to the emergence of the plant pathogenic bacteria Xylella fastidiosa (Lysobacterales: Lysobacteraceae) (Xf). Xf comprises five main subspecies and induces severe physiological symptoms in a diverse set of host plant species by limiting xylem function. Xylem-feeding insect vectors can transmit Xf to host plants, spreading disease and reducing fruit yield. Here I studied Xf prevalence on small fruit farms to determine Xf host plant and vector infective status to the Xf subspecies level. I detected Xf in 28 host plants in South Carolina containing either Xf subspecies fastidiosa …

Don’T Be A Big Baby: Phenotypic Novelty In The Facultative Paedomorph Ambystoma Talpoideum., Andrew S. Kanes

All Theses

This thesis explores the relationship between heterokairy and the skin microbiota of amphibians, specifically focusing on the facultatively paedomorphic salamander, Ambystoma talpoideum. Heterokairy refers to variations in developmental timing that generate phenotypic novelty within life stages. The first chapter investigates how reordering metamorphosis relative to sexual maturation affects the skin microbiota in this species. By sampling different life stages—larval, paedomorphic, and metamorphic—results show the emergence of novel microbial combinations, resulting from both microbial gain and loss in the heterokairic paedomorphic stage. These findings suggest that changes in microbial communities may lead to phenotypic novelty and have broader eco-evolutionary implications. …

Application Of Remote Sensing In Mapping Callery Pear Distribution And Biomass Estimation In The Southeastern United States, Kavi Raj Awasthi

All Theses

Callery pear (Pyrus calleryana Decne. Rosaceae) is a rapidly spreading invasive species in the southeast United States (US) that hampers the function of native tree species and the forest ecosystem. Native to Southeast Asia, the species has encroached on the natural and secondary vegetation habitats of the Southern US in recent years. This study attempted to 1) map the Callery pear across the southeast US and 2) model the aboveground biomass (AGB) of individual Callery pear trees.

We used Callery pear locations and winter imageries of Sentinel-2, Sentinel-1, L-band radar, and terrain imagery for mapping purposes. In addition, we …

A Review On Treatment, Prevention, And Research Of Worldwide Parasitic Disease: Toxoplasmosis, Hannah Skinner

All Theses

Toxoplasmosis is a worldwide, yet often overlooked, parasitic disease caused by the protozoan parasite, Toxoplasma gondii. The parasite is capable of infecting humans and almost all warm-blooded vertebrates. Over a million people are infected in the United States with toxoplasmosis each year. Most infections are asymptomatic but severe cases can lead to vision impairment, neurological disorders, and congenital birth defects. T. gondii is transmitted through undercooked meat, infected feline feces, or contaminated water and soil. The burden of T. gondii infection is most notable in livestock, felines, and immunocompromised humans. Despite being a widespread disease, toxoplasmosis is neglected in …

Thymosin Beta-4: A Novel Treatment For Acanthamoeba Keratitis, Victoria A. Murphey

All Theses

Acanthamoeba keratitis (AK) is a severe corneal infection caused by the free-living amoeba, Acanthamoeba. Both stages of its life cycle (trophozoite and cyst) cause infection, primarily in contact lens wearers. It is often misdiagnosed, leading to delayed treatment and poor clinical outcomes. Current treatment options for AK rely on anti-amoebic agents that are toxic to corneal epithelial cells. Further, no single drug can eliminate both forms of the amoeba. Approximately 30% of all AK infections require a corneal transplant.

Thymosin beta-4 (Tβ4) is a G-actin binding protein synthesized in almost all human cells, including corneal epithelial cells. Tβ4 exhibits …

The Effect Corrugated Fiberboard Has On The Environment In Terms Of Compostability, Plant Growth, And Per-And-Poly-Fluoroalkyl Substance Contamination, Meredith Clem

All Theses

The “Lasagna Method” is a popular and sustainably perceived technique that many gardeners use as a free and convenient method to dispose of cardboard (corrugated fiberboard) packaging while also improving their gardens. Many claim that this creates benefits for your garden, but many others have proposed their concerns, including the safety of the practice. This research analyzes the compostability of cardboard, the effect it has on plant growth and development of lettuce, and its contamination of a prominent chemical that has known negative effects on human health. The results of these studies showed that cardboard, as it is currently produced …

Evaluating The Role Of Electrostatic Forces In The Adsorption Of Perfluoroalkyl Substances To Granular Activated Carbon, Thomas A. Mccall

All Theses

In response to new drinking water regulations announced by the EPA in 2024, many municipalities will need to monitor concentrations and implement new treatments to address PFAS, a class of anthropogenic organic contaminants. A leading technology for this application is GAC adsorption due to its familiarity and availability. The adsorption of four PFAS species (PFBA, PFBS, PFOA and PFOS) to Calgon Filtrasorb400 (F400) was evaluated for a range of pH and ionic strength conditions to investigate the role of electrostatic interactions in adsorption capacity. These PFAS are all strong acids, so pH was used as a control for GAC surface …

Chromosome Evolution Model Reveals Hidden Variation In Karyotype-Driven Speciation In Ferns, Thomas Buchloh

All Theses

Because karyotype change commonly generates reproductive isolation between diverging species, rapid karyotype evolution, like that seen in plants, may increase the total rate of diversification. However, few studies have investigated this predicted relationship. Tests of this prediction have identified a positive correlation between the rate of karyotype change (specifically whole genome duplications) and diversification, but tests have been restricted to relatively small and young clades. Fortunately, novel macroevolutionary models have recently become available to investigate patterns of karyotype evolution in large phylogenies, providing new opportunity to investigate variation in karyotype driven diversification. Ferns are one of the most karyotype rich …

Assessing The Viability Of A Proposed Third-Party Reusable Foodware System: An Environmental And Economic Comparison, Margaret Milani

All Theses

The United States generates 73 million tons of plastic waste annually, with over 6% attributed to the restaurant industry. The EPA recommends that restaurants reduce their plastic waste by transitioning to reusable foodware items. Previous studies have shown that using a single reusable foodware item, such as a cup, can result in lower environmental impacts and greater economic benefits compared to single-use plastic alternatives. However, the upfront costs of purchasing reusables, commercial dishwashers, and other associated expenses remain significant barriers for restaurants, leading many to continue using single-use plastic foodware. This study aims to analyze and compare the environmental impacts …

Healthy Dietary Patterns’ Impact On Depressive Symptoms: A Meta-Analysis Of Randomized Controlled Trials, Angela Encardone

All Theses

As the search for innovative mental health treatments expands, dietary interventions have emerged as a promising approach for alleviating depressive symptoms. This thesis presents a meta-analysis of randomized controlled trials (RCTs) examining the efficacy of whole-of-diet nutritional interventions in reducing depression symptoms among adults. Grounded in Bronfenbrenner’s Bioecological Model and Engel’s Biopsychosocial Model, this study explores the intersection of biological, psychological, and social factors that influence mental health outcomes. A systematic review identified six eligible RCTs assessing the impact of healthy dietary patterns, including the Mediterranean diet and other plant-based interventions, on depression symptoms. A random-effects meta-analysis revealed a significant …

Spatiotemporal Changes In The Imperiled And Diverse Mussel Assemblage Of The Spring River In Kansas In Response To Known And Emerging Pollutants, Joshua Holloway

Electronic Theses & Dissertations

Kansas was historically home to 50 species of freshwater mussels, of which 42 remain extant. The Spring River of southeastern Kansas is home to 34 of the remaining 42 mussels, many of which are imperiled. Elevated metal concentrations that resulted from past lead and zinc mining in the Tri-State Mining District historically imperiled Spring River mussels, but this threat has abated over the last 20 years. Ammonia pollution may have replaced the threat of metals, however, with potentially severe consequences for Spring River mussels. Our objectives were to evaluate contemporary mussel assemblage structure, in addition to spatiotemporal trends in the …

The Effect Of Implementing A Development Program Using Mental Imagery On The Time To Master Psychomotor Skills, Randal Agee

Dissertations

Psychomotor skills development programs that use mental imagery have been demonstrated to help individuals acquire psychomotor skills (Anu et al., 2020; Seymore-Walsh et al., 2020; Toth et al., 2020). The commercial trucking industry is presently experiencing a global shortage of qualified truck drivers (American Trucking Association, 2019; Staats et al., 2017). One reason individuals are avoiding or leaving careers as commercial truck drivers is the time spent away from home (Staats et al., 2017). A couple logistical strategies have been developed to improve the situation (Mittal et al., 2018; Prime Inc, 2024); however, for the solutions to be sustainable, the …

Unraveling The Antiviral Mechanisms Of Bat Cells: Processing Of Genomic Rnas By Dicer In Bat Cells Limits Sars-Cov-2 Replication, Iyanuoluwani Owolabi

Dissertations

Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms are proposed to contribute to the tolerance of bats to these pathogens. This study investigates the response of bat cells to double-stranded RNA generated by SARS-CoV-2 replication. Here, we found the involvement of Dicer in the processing of viral genomic RNAs during SARS-CoV-2 infection. Examining RNA sequencing of infected cells, small-interfering RNA (siRNA)-like fragments were found derived from viral RNAs. Depletion of Dicer showed a reduction in these RNAs and an increase in viral loads suggesting unlike other mammals, bats may use Dicer …

Determining The Influence Of A Low-Head Dam On Macroinvertebrate Communities In The Bouie River, Natalie Santiago

Honors Theses

The Bouie River is a tributary of the Leaf River and belongs to the Pascagoula River watershed, the latter of which has no flow impediments in the form of sills or dams. The Bouie River has been heavily altered in the lower 10 river kilometers (rkm) from aggregate mining (creating a series of deep gravel pits) and the presence of an earthen and concrete low-head dam (sill) located 6 rkm from the mouth of the river. However, despite these alterations, the immediate stretch of river below the low-head dam is a known spawning habitat for diadromous fish species, including the …

Examining The Molecular Mechanisms Of Glucagon-Like Peptide-1 Receptor Agonists In Cancer Cell Biology, Oliver G. Sabet

Honors Scholar Theses

Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are synthetic analogs of glucagon-like peptide-1 (GLP-1) used to treat obesity and diabetes by reducing blood glucose levels and appetite. While early rodent studies suggested a link between GLP-1RAs and thyroid cancer pathogenesis, evidence in humans remains inconclusive, with randomized controlled trials not supporting that link. Due to the rapid, widespread use of these drugs, concerns have expanded to other obesity-associated cancers, with conflicting findings on their role in cancer progression. With this contradictory evidence in a novel intersection of obesity medicine and oncology, this literature review aims to summarize current understandings between GLP-1-RAs and …

The Role Of Myosin L In The Survival Of Toxoplasma Gondii And The Importance Of Mlc1 For In Vitro And In Vivo Myosin F Motility, Victoria K. Yi

Honors Scholar Theses

My thesis discusses the work that I have conducted on two myosins in Toxoplasma gondii, which is an obligate intracellular parasite that has chronically infected around 10% of the American population. It causes toxoplasmosis and severe health consequences for individuals with compromised immune systems or when infections occur in utero. However, immunocompetent individuals are asymptomatic or present with subclinical symptoms. In the Heaslip lab, I investigated two myosins in the parasite: Myosin L and Myosin F. MyoL is an uncharacterized motor that localizes at the apical end of the parasite. It was shown to be important for parasite …

Identifying Cognitive, Affective, And Developmental Mechanisms Linking Threat And Deprivation With Adolescent Psychopathology, Ekaterina Sadikova, David G. Weissman, Maya L. Rosen, Elise Robinson, Liliana J. Lengua, Margaret A. Sheridan, Henning Tiemeier, Katie A. Mclaughlin

Neuroscience: Faculty Publications

Background: The mechanisms linking early-life adversity with psychopathology over the life-course are complex. In this prospective study, we collectively examined cognitive, affective, and developmental mediators previously found to individually link childhood threat and deprivation experiences to adolescent psychopathology to identify the most potent mechanisms. Methods: Data came from a community sample of 227 children (mean child age 11.5 ± 0.5 years, 48.5% female) from the Seattle metro area with recruitment designed to reflect diversity in family income. Candidate mechanisms included self-rated pubertal development and task-measured attention bias to threat, emotion regulation, theory of mind, fear learning, inhibitory control, language ability, …

Assessment Of Lake Salvador Shoreline Dynamics To Support Restoration Of Submerged Aquatic Vegetation In Jean Lafitte National Historical Park And Preserve, Louisiana, Lydia V. Dipaola

LSU New Orleans Theses and Dissertations

High wave energy degrades shoreline habitat between Lake Salvador and Jean Lafitte National Historical Park and Preserve (JELA), Louisiana. JELA constructed a northern breakwater in 2004, and a southern breakwater in 2024 to protect the shoreline and promote restoration of submerged aquatic vegetation (SAV). This study analyzes Sentinel-2 imagery to quantify coastal loss along the JELA-Lake Salvador using the Change Polygon Approach, assessing annual, cumulative and major storm event-associated shoreline changes for January 2017-2025. SAV cover, nekton diversity, wave energy, and turbidity were measured before-and-after southern breakwater construction to monitor ecological and environmental outcomes. The JELA-Lake Salvador shoreline has lost …

Direct Synthesis Of Cerium Oxide Nanoparticles In Reducing Sugars: Enhanced Ce3+ Surface Density For Biological Application, Balaashwin Babu, Elayaraja Kolanthai, Craig J. Neal, Samarah V. Harb, Udit Kumar, Yifei Fu, Sudipta Seal

Rowan-Virtua Research Day

Cerium oxide nanoparticles (CNPs) are recognized for their ability to scavenge reactive oxygen species (ROS), making them promising candidates for mitigating oxidative damage in biomedical applications. However, many conventional synthesis methods limit the formation of Ce³⁺ states, which are critical for this antioxidant function. Building upon previous findings that glucose can promote Ce³⁺ formation, this study investigates the broader impact of both reducing (glucose, fructose, galactose) and non-reducing (dextran, sucrose, cyclodextrin) natural sugars on CNP synthesis. CNPs were synthesized in the presence of these sugars, and the surface densities of Ce³⁺ were measured. Results showed that reducing sugars significantly increased …

Registration Of 'R19c-1012': A High-Yielding Soybean Cultivar With Improved Flooding Tolerance At Early Vegetative Stages, Caio Canella Vieira, Rafael Marmo, C. Wu, Liliana Florez-Palacios, Andrea Acuna, D. Harrison, D. Rogers, Leandro Mozzoni, T. Roberts, J. F. Carlin, Travis Faske, F. Lin, G. Shannon, H. T. Nguyen

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

'R19C-1012' (Reg. no. CV-564, PI 707837) is a high-yielding, conventional maturity group Mid-IV (Relative maturity 4.6) soybean [Glycine max (L.) Merr.] cultivar with improved flooding tolerance at V2-V4 vegetative growth stages. It was developed and released by the University of Arkansas System-Division of Agriculture Research & Extension Center in 2024. R19C-1012 has an indeterminate growth habit, purple flowers, gray pubescence, brown pods, and seed with an imperfect black hilum. From 2019 to 2023, R19C-1012 was evaluated across 40 irrigated environments, with an average grain yield of 4302 kg ha-1, representing 93% and 99% relative yield of the Xtend and non-Xtend …

A Bishnoi Woman’S Odhani: Gendered Labor, Environmental Ethics, And Textile Traditions In Rajasthan, Nidhi Patel

Department of Anthropology

This report examines an embroidered odhani (woman’s shawl) attributed to a Bishnoi artisan from Rajasthan, India. The odhani is housed in the May Weber Ethnographic Study Collection at Loyola University Chicago. Close analysis of technique and contextual evidence is used to reattribute the textile, which was originally mislabeled and situates it within the contexts of gendered labor and environmental ethics within the Bishnoi community. Utilizing the frameworks of materialist and eco-spiritual feminism, the report argues that the odhani functions as a site of ecological resistance, cultural transmission, and women’s embodied knowledge, as well as a garment.

Movement Ecology Of Sandhills Chub, A Carolina Endemic, Zachary Adam Ramsey

Electronic Theses and Dissertations

Fish movement studies have traditionally focused on species that support commercial and recreational fisheries, but recently more focus has been given to non-game, stream fishes because of their recognized importance in maintaining biodiversity within these ecosystems. Stream fish movement is described as being “restricted” and following a leptokurtic distribution via diffusive spread related to environmental and biological factors. Many studies of stream fish movement ecology in North America have occurred in high-gradient streams with coarse substrate in mountains or in streams of the interior plains. Sandhills streams of the southeastern USA are characterized as warm-water, low gradient, and dominated by …

Flow Field Surrounding Bluegill (Lepomis Macrochirus) During Suction-Feeding, Jensine Caroline Coggin

Electronic Theses and Dissertations

Suction-feeding is a common method for capturing prey by aquatic organisms and contributed to the diversification of fishes by enabling them to consume a wide range of prey. Suction-feeding is a complex fish-fluid interaction governed by various hydrodynamic forces: inertia, unsteady, viscous, and pressure gradients. These forces are described by the coupling between the flow physics equations (Navier-Stokes) and the dynamic behavior of the fish (motion and forces). However, the distribution of the pressure field that drives this process, and the extent to which suction-feeding is three-dimensional, remain underexplored. I estimated the pressure within the flow field surrounding the mouth …

Heterotopias Of Confinement: Zoo Architecture And The Loss Of Elephant Agency, Clayton Gill Beasley

Electronic Theses and Dissertations

Zoos functions as a site where human-animal relationships are structured through built environments that mediate control and representation. This study examines zoos as heterotopias—spaces where power and surveillance are negotiated between human observers and captive nonhuman animals. While zoos are often framed as spaces of conservation and education, their architectural design reinforces hierarchies of observation and confinement, shaping both human perceptions of nature and the lived experiences of nonhuman animals. This research focuses on Asian elephants as a critical species within this framework, exploring how their captivity illuminates tensions between human-imposed structures and nonhuman autonomy. Drawing from theories of heterotopia, …

Implications Of Type Iv Pilus Retraction For Dna Uptake By Acinetobacter Baumannii, Yafan Yu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Horizontal gene transfer (HGT) via natural competence allows bacteria to incorporate extracellular DNA (eDNA) into their genomes, facilitating genetic diversity and adaptation. In Acinetobacter species, natural competence depends on DNA binding and uptake mediated by type IV pili (T4P). T4P are dynamic extracellular filaments composed primarily of the major pilin subunit PilA, along with a few minor subunits. T4P drive a range of cellular functions including twitching motility, biofilm formation, and DNA uptake, with some functions dependent on pilus retraction and others not. However, how Acinetobacter T4P bind DNA and how T4P/eDNA interactions impact biofilm formation remain unclear. Here, I …

Modifying The Comb, Rethinking The Install: Integrated Approaches To Honey Bee Colony Establishment And Winter Survival, Shelby A. Kittle

Department of Entomology: Dissertations, Theses, and Student Research

The European, or Western honey bee (Apis mellifera L.) plays a vital role in global ecosystems and agriculture, providing essential pollination services and producing valuable hive products. However, honey bee colony losses have escalated worldwide, but particularly across the United States due to various stressors, including biotic and abiotic factors, mismanagement, and environmental challenges. This thesis investigates key challenges in honey bee management and explores solutions aimed at improving colony health and productivity.

Chapter 1 provides an overview of the beekeeping industry, detailing its economic importance, services, and products, as well as the many challenges faced by beekeepers. It …

Mechanisms Of Perennial Sorghum Resistance To Sugarcane Aphid Infestation, Esha Kaler

Department of Entomology: Dissertations, Theses, and Student Research

Sorghum, Sorghum bicolor (L.) Moench, the fifth most-grown cereal crop in the world, is a versatile crop, grown across different agro-ecological regions. Sorghum has numerous uses ranging from food and forage purposes to biofuel production. Sugarcane aphid (SCA; Melanaphis sacchari) is a major threat to sorghum production, accounting for 50% to 100% yield loss under heavy infestations. Perennial sorghum can be a sustainable and climate-resilient alternative to the current cereal farming systems. The development of perennial sorghum offers numerous benefits, such as reduced economic inputs, more carbon sequestration, better soil health, and sustainable yields. We employed host plant resistance …