Life Sciences Commons^™

Improving Spatial Prioritisation For Remote Marine Regions: Optimising Biodiversity Conservation And Sustainable Development Trade-Offs, Cordelai H. Moore, Ben T. Radford, Hugh P. Possingham, Andrew Heyward, Romola R. Stewart, Matthew E. Watts, Jim Prescott, Stephen J. Newman, Euan S. Harvey, Rebecca Fisher, Clay W. Bryce, Ryan J. Lowe, Oliver Berry, Alexis Espinosa- Gayosso, Errol Sporer, Thor Saunders

Fisheries Research Articles

Creating large conservation zones in remote areas, with less intense stakeholder overlap and limited environmental information, requires periodic review to ensure zonation mitigates primary threats and fill gaps in representation, while achieving conservation targets. Follow-up reviews can utilise improved methods and data, potentially identifying new planning options yielding a desirable balance between stakeholder interests. This research explored a marine zoning system in north-west Australia–a biodiverse area with poorly documented biota. Although remote, it is economically significant (i.e. petroleum extraction and fishing). Stakeholder engagement was used to source the best available biodiversity and socio-economic data and advanced spatial analyses produced 765 …

Household, Psychosocial, And Individual-Level Factors Associated With Fruit, Vegetable, And Fiber Intake Among Low-Income Urban African American Youth., Angela Cristina Bizzotto Trude, Anna Yevgenyevna Kharmats, Kristen Marie Hurley, Elizabeth Anderson Steeves, Sameera A Talegawkar, Joel Gittelsohn

Exercise and Nutrition Sciences Faculty Publications

BACKGROUND: Childhood obesity, one of the greatest challenges to public health, disproportionately affects low-income urban minority populations. Fruits and vegetables (FV) are nutrient dense foods that may be inversely associated with excessive weight gain. We aimed to identify the individual characteristic, psychosocial, and household factors influencing FV and fiber consumption in low-income African-American (AA) youth in Baltimore, MD.

METHODS: Cross-sectional analysis of data collected from 285 low-income AA caregiver-youth (age range: 10-14 y) dyads participating in the baseline evaluation of the B'More Healthy Communities for Kids obesity prevention trial. The Kid's Block FFQ was used to estimate daily intakes of …

Raising Nebraska: Engaging The Public In Ways That They Value, Chuck Hibberd

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Raising Nebraska A partnership: •Nebraska State Fair •Nebraska Extension •Nebraska Department of Agriculture With a purpose: •Demonstrate the science and art of Nebraska agriculture •Learn from consumers

Prescribing irrigation –conserving water

Walkable Nebraska map -elevation change, waterways, precipitation and crops/livestock

Trusted Voice Videos –Top 5 & Bottom 5

Interactive Dinner Table -- Dinner Table –Entrée, Drink, & Side Selection

Exit Poll -How many things did you learn today?

Conclusions •Participants are telling us what they want to learn more about •Participant responses provide insight on content offerings •Interactive, digitally-based learning venues are effective

Tell Me A Science Story: Transforming Engagement With Science Using Creative Nonfiction, Janell C. Walther

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

“I definitely felt more engaged with the first one [creative nonfiction]and less tuned out because I had somebody interpreting their response for me. So when he was like “Oh, I was surprised” then I think “Oh, so was I, I was also surprised by the piece of information.” So I was more engaged with it personally even if I didn't retain all the numbers... “

“[The story] seemed more relatable I guess, as a normal, average, everyday person than a whole bunch of scientists talking about facts. Where the facts were still in here, the way that it was written …

Science Not Communicated Is Science Not Done: Present Your Science Powerfully, Melissa Marshall

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Great communication ignites a chain reaction. Discussion: Identify the best presenter of science that you have seen. What made that speaker so effective?

To excel in your presentations, you will need content , passion, and a sharp sense of the audience.

Science not communicated is science not done. Presenting your science powerfully requires mastery of three skills: Be Audience Centered, Filter and focus, Show your science

Becoming an audience focused speaker is one of the most powerful skills you can adopt. Returning to points of common ground satisfies audiences of different technical backgrounds.

Successful technical presentations require you to filter …

How To Talk About Science In The Face Of Religious Mistrust, Clay Farris Naff

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

King Ashoka (268to 232 BCE). Ralph Waldo Emerson. Homer Simpson. Us and THEM. Timothy Gay, UNL Prof. of Physics. Ken Miller, Prof. of Biology, Brown. Richard Dawkins. Ken Ham, Answers in Genesis.

THEM = Truthful Humble Evidential Memorable

Theistic Evolution. Human Babies Barely Fit. The right & left laryngeal nerves take wildly differing paths. Progeria. General Theory of Relativity. Sun’s Gravity Bends Starlight. GPS & Relativity. Sugar vs. Fat. U.S. stock market. Pope. Dalai Lama. Epicurus.

An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay For Specific Detection Of Zika Virus, Dhiraj Acharya, Pradip Bastola, Linda Le, Amber M. Paul, Estefania Fernandez, Michael S. Diamond, Wujian Miao, Fengwei Bai

Faculty Publications

Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs …

Expert Elicitation On The Uncertainties Associated With Chronic Wasting Disease, Michael G. Tyshenko, Tamer Oraby, Shalu Darshan, Margit Westphal, Maxine C. Croteau, Willy Aspinall, Susie Elsaadany, Daniel Krewski, Neil Cashman

School of Mathematical & Statistical Sciences Faculty Publications

A high degree of uncertainty exists for chronic wasting disease (CWD) transmission factors in farmed and wild cervids. Evaluating the factors is important as it helps to inform future risk management strategies. Expert opinion is often used to assist decision making in a number of health, science, and technology domains where data may be sparse or missing. Using the "Classical Model" of elicitation, a group of experts was asked to estimate the most likely values for several risk factors affecting CWD transmission. The formalized expert elicitation helped structure the issues and hence provide a rational basis for estimating some transmission …

Effect Of Pulse Flours On The Physiochemical Characteristics And Sensory Acceptance Of Baked Crackers., Kim Millar, Catherine Barry-Ryan, Róisín Burke, Sinead Mccarthy, Eimear Gallagher

Conference papers

Pulse flours offer nutritional alternatives to wheat flour in the production of baked snacks due to their high protein and fibre levels and low glycaemic index. In this study, broad-bean (Vicia faba), yellow-pea and green-pea (Pisum sativum) flours were each blended with wheat flour at 40% in the formulation of chemically-leavened crackers. The effects of flour type and baking time on the physiochemical properties, sensory acceptability, nutritional composition and antioxidant activity of the crackers were observed in comparison to 100% wheat crackers. Broad-bean crackers had the highest protein content and antioxidant activity (13g/100g DM and 38.8 …

An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay For Specific Detection Of Zika Virus, Amber M. Paul, Dhiraj Acharya, Pradip Bastola, Linda Le, Estenfani Fernandez, Michael S. Diamond, Wujian Miao, Fengwei Bai

Publications

Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs …

Bacteria And The Cellular Basis Of Consciousness, Michael L. Woodruff

Animal Sentience

According to Reber’s theory, the Cellular Basis of Consciousness (CBC), sentience originates as bio-sensitivity in unicellular organisms. For this reason, Reber regards sentience as evolutionarily foundational. Many bacteria show chemotaxis and, thus, according to CBC, they are sentient. Analysis of the genetic mechanisms underlying bacterial chemotaxis indicates that sentience has no explanatory power in this case. Genetic analysis also fails to show species continuity underlying bio-sensitivity in bacteria and bio-sensitivity in species with nervous systems, so it does not seem that sentience is evolutionary foundational. CBC is rejected on these grounds.

Unity Of Consciousness In Animals, Bernard E. Rollin

Animal Sentience

Both Descartes the rationalist and Hume the empiricist, polar opposites philosophically, denied the unity and continuity of animal mind. Kant pointed out that the presence of retrievable memories entails unity of consciousness. Rowlands now argues that animals too have unity of consciousness.

Butheoloides Grosseri Sp. N. (Scorpiones: Buthidae) From Uganda, František Kovařík

Euscorpius

A new species Butheoloides grosseri sp. n. is described and fully complemented with color photos of female holotype, as well as its habitat. This is the first species assigned to this genus from Uganda and represents southern limits of genus Butheoloides distribution.

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Viii. Pandinops Birula, 1913 (Scorpionidae), With Description Of Two New Species, František Kovařík

Euscorpius

Complete Pandinops trichobothrial pattern and spiniform formula of tarsomeres of legs are published for the first time. P. friedrichi sp. n. from Somalia and P. turieli sp. n. from Ethiopia and Kenya are described. Information is provided about all Pandinops species, their taxonomy, and distribution, and P. turieli sp. n. discovered during scorpiological expeditions in 2011–2016 is fully complemented with color photos of live and preserved specimens, as well as its habitat.

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Vii. Parabuthus Pocock, 1890 (Buthidae), With Description Of P. Hamar Sp. N. And P. Kajibu Sp. N. From Ethiopia, František Kovařík, Graeme Lowe, Jana Plíšková, František Šťáhlavský

Euscorpius

All Parabuthus species from Eritrea, Ethiopia, and Somaliland were newly collected and are revised for the first time. The complex of Parabuthus liosoma is split into three sibling species with separate areas of distribution: P. abyssinicus Pocock, 1901 (Eritrea, Djibouti, central and north-eastern parts of Ethiopia), P. liosoma (Ehrenberg, 1828) (Yemen and Saudi Arabia), and P. maximus Werner, 1913 (Tanzania and Kenya). P. hamar sp. n. and P. kajibu sp. n., discovered during scorpiological expeditions in 2011–2016, are described. Information is provided about all Parabuthus species from the Horn of Africa, their taxonomy, distribution, and ecology, fully complemented with …

Does The Seal Licensing System In Scotland Have A Negative Impact On Seal Welfare?, Laetitia Nunny, Fritha Langford, Mark P. Simmonds

Wildlife Population Management Collection

This study examined the licensing system that permits seal shooting in Scotland, which was established under Part 6 Conservation of Seals of the Marine (Scotland) Act 2010. Four approaches were used: data were collated and analyzed from both the Scottish Government and Scottish Marine Animal Stranding Scheme; a survey was sent to current license holders and informal interviews were conducted with key stakeholder types. Between February 2011 and the end of October 2015, 1229 gray seals, and 275 common seals were reported shot under license to the Scottish Government. The numbers of seals reported as shot has reduced year-on-year since …

Does The Seal Licensing System In Scotland Have A Negative Impact On Seal Welfare?, Laetitia Nunny, Fritha Langford, Mark P. Simmonds

Aquaculture and Fisheries Collection

This study examined the licensing system that permits seal shooting in Scotland, which was established under Part 6 Conservation of Seals of the Marine (Scotland) Act 2010. Four approaches were used: data were collated and analyzed from both the Scottish Government and Scottish Marine Animal Stranding Scheme; a survey was sent to current license holders and informal interviews were conducted with key stakeholder types. Between February 2011 and the end of October 2015, 1229 gray seals, and 275 common seals were reported shot under license to the Scottish Government. The numbers of seals reported as shot has reduced year-on-year since …

Population Dynamics And Genetic Differentiation Of The Threatened Freshwater Mussel Species Fusconaia Askewi, Fusconaia Lananensis, And Pleurobema Riddellii, Edith D. Plants-Paris

Biology Theses

North America has the most diverse freshwater mussel fauna in the world with approximately 300 species; unfortunately, extinction rates for freshwater mussels rivals the rates of many other groups of organisms. Population-level natural life history data is essential in the management of species of conservation concern, yet basic information about freshwater mussel life-history and demographic traits are unknown for many species. To further complicate matters, taxonomic uncertainty exists among some members of the group. The work detailed herein had two goals: to gain further understanding of the taxonomic relationship between Fusconaia lananensis and F. askewi by sequencing genes that had …

Nanosecond Dynamics Of Gαi1 Bound To Nucleotides Or Ric-8a, A Gα Chaperone With Gef Activity, Labe A. Black, Celestine J. Thomas, Gwendolyn N. Nix, Michelle C. Terwilliger, Stephen R. Sprang, J B. Ross

Biological Sciences Faculty Publications

Resistance to Inhibitors of Cholinesterase A (Ric-8A) is a 60-kDa cytosolic protein that has chaperone and guanine nucleotide exchange (GEF) activity toward heterotrimeric G protein α subunits of the i, q, and 12/13 classes, catalyzing the release of GDP from Gα and subsequent binding of GTP. In the absence of GTP or GTP analogs, and subsequent to GDP release, Gα forms a stable nucleotide-free complex with Ric-8A. In this study, time-resolved fluorescence anisotropy measurements were employed to detect local motions of Gαi1 labeled at selected sites with Alexa 488 (C5) fluorescent dye (Ax) in the GDP, GTPγS (collectively, GXP), and …

U.S. Drought Monitor, August 23, 2016, Brad Rippey

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for August 23, 2016 (8/23/16) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Belowground Rhizomes In Paleosols: The Hidden Half Of An Early Devonian Vascular Plant, Jinzhuang Xue, Zhenzhen Deng, Pu Huang, Kangjun Huang, Michael J. Benton, Ying Cui

Dartmouth Scholarship

The colonization of terrestrial environments by rooted vascular plants had far-reaching impacts on the Earth system. However, the belowground structures of early vascular plants are rarely documented, and thus the plant−soil interactions in early terrestrial ecosystems are poorly understood. Here we report the earliest rooted paleosols (fossil soils) in Asia from Early Devonian deposits of Yunnan, China. Plant traces are extensive within the soil and occur as complex network-like structures, which are interpreted as representing long-lived, belowground rhizomes of the basal lycopsid Drepanophycus. The rhizomes produced large clones and helped the plant survive frequent sediment burial in well-drained soils …

The Importance Of Ambient Temperature To Growth And The Induction Of Flowering, C. R. Mcclung, Ping Lou, Victor Hermand, Jin A. Kim

Dartmouth Scholarship

Plant development is exquisitely sensitive to the environment. Light quantity, quality, and duration (photoperiod) have profound effects on vegetative morphology and flowering time. Recent studies have demonstrated that ambient temperature is a similarly potent stimulus influencing morphology and flowering. In Arabidopsis, ambient temperatures that are high, but not so high as to induce a heat stress response, confer morphological changes that resemble the shade avoidance syndrome. Similarly, these high but not stressful temperatures can accelerate flowering under short day conditions as effectively as exposure to long days. Photoperiodic flowering entails a series of external coincidences, in which environmental cycles of …

Baby, This World Is Cruel, Nytesia S. Ross

Journal of Multicultural Affairs

This poem is about a woman's fear of bringing a child into this world.

Joint Action Effects Of Emamectin Benzoate And Cypermethrin On The Marine Copepod Tigriopus Californicus, Lindsay Schultz, Michael Kelly

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

This project examined the effect of two pesticides on the marine copepod Tigriopus californicus. The pesticides are emamectin benzoate and cypermethrin, which are commonly used in salmon aquaculture to treat the salmon for sea lice, a type of parasitic copepod. The results of this study indicate that these pesticides are toxic to the free-living T. californicus at very low dosage levels when used individually. When the pesticides are used in combination, the mortality rate is similar to when they are used individually, but there is an additional paralytic effect on the animals. All animals exposed to any of the pesticide …

The Farm Newsletter, Usu Student Organic Farm

USU Student Organic Farm Newsletter

A Note on Carrots

Included in your shares with the carrots are the carrot greens. Though usually discarded, carrot tops are actually edible and highly nutritious. They can been eaten raw in salads, cooked into soups, or used in a variety of other ways. Important to note however is that due to the natural biological processes in the plant, if your carrot tops are left attached to the roots for an extended time they will make your carrots soft. This is because the plant is still alive and the leaves are still pulling moisture from the roots.

Eelgrass Distribution In The Great Bay Estuary For 2014, Frederick T. Short

PREP Reports & Publications

See also: "Eelgrass/Macroalgae Discussion Primer for TAC Activities 2016-2017" http://scholars.unh.edu/prep/355

Eelgrass (Zostera marina L.) is an essential habitat for the Great Bay Estuary (GBE) because it is the basis of an estuarine food web that supports many of the recreationally, commercially and ecologically important species in the estuary and beyond. Eelgrass provides food for ducks, geese and swans, as well as food, nursery habitat, and shelter for juvenile fish and shellfish. Eelgrass filters estuarine waters and improves water clarity, removing both nutrients and suspended sediments from the water column; its roots and rhizomes bind and hold sediments in place, thereby …

How Did The Deepwater Horizon Oil Spill Impact Deep-Sea Ecosystems?, Charles R. Fisher, Paul A. Montagna, Tracey T. Sutton

C-IMAGE Publications

Approximately 90% of the volume of the Gulf of Mexico is contained in water deeper than 200 m, a region where the Deepwater Horizon (DWH) blowout had more impact on ecosystems than any previous oil spill. The remoteness and relative inaccessibility of the deep sea makes documenting even acute impacts to the animals that live in this realm difficult. This article reviews Natural Resource Damage Assessment studies and follow-up work funded as part of the Gulf of Mexico Research Initiative that targeted deepwater pelagic and benthic fauna. Oil was incorporated into the pelagic food web, and a reduction in planktonic …

Responses Of Microbial Communities To Hydrocarbon Exposures, Samantha B. Joye, Sara Kleindienst, Jack A. Gilbert, Kim M. Handley, Pamela Weisenhorn, Will A. Overholt, Joel E. Kostka

C-IMAGE Publications

The responses of microbial communities to hydrocarbon exposures are complex and variable, driven to a large extent by the nature of hydrocarbon infusion, local environmental conditions, and factors that regulate microbial physiology (e.g., substrate and nutrient availability). Although present at low abundance in the ocean, hydrocarbon-degrading seed populations are widely distributed, and they respond rapidly to hydrocarbon inputs at natural and anthropogenic sources. Microbiomes from environments impacted by hydrocarbon discharge may appear similar at a higher taxonomic rank (e.g., genus level) but diverge at increasing phylogenetic resolution (e.g., sub-OTU [operational taxonomic unit] levels). Such subtle changes are detectable by computational …

Chromosomer: A Reference-Based Genome Arrangement Tool For Producing Draft Chromosome Sequences, Gaik Tamazian, Pavel Dobrynin, Ksenia Krasheninnikova, Aleksey Komissarov, Klaus-Peter Koepfli, Stephen J. O'Brien

Biology Faculty Articles

Background: As the number of sequenced genomes rapidly increases, chromosome assembly is becoming an even more crucial step of any genome study. Since de novo chromosome assemblies are confounded by repeat-mediated artifacts, reference-assisted assemblies that use comparative inference have become widely used, prompting the development of several reference-assisted assembly programs for prokaryotic and eukaryotic genomes.

Findings: We developed Chromosomer – a reference-based genome arrangement tool, which rapidly builds chromosomes from genome contigs or scaffolds using their alignments to a reference genome of a closely related species. Chromosomer does not require mate-pair libraries and it offers a number of auxiliary tools …

Examining The Effects Of Chromatic Aberration, Object Distance, And Eye Shape On Image-Formation In The Mirror-Based Eyes Of The Bay Scallop Argopecten Irradians, Daniel Isaac Speiser, Yakir Luc Gagnon, Raghav K. Chhetri, Amy L. Oldenburg, Sönke Johnsen

Faculty Publications

The eyes of scallops form images using a concave spherical mirror and contain two separate retinas, one layered on top of the other. Behavioral and electrophysiological studies indicate that the images formed by these eyes have angular resolutions of about 28. Based on previous ray-tracing models, it has been thought that the more distal of the two retinas lies near the focal point of the mirror and that the proximal retina, positioned closer to the mirror at the back of the eye, receives light that is out-of-focus. Here, we propose three mechanisms through which both retinas may receive focused light: …