Life Sciences Commons^™

Establishment Of A Quantitative Real-Time Pcr For Detecting Vvha Gene In Vibrio Vulnificus, Li Dandan, Xu Yigang, Qiu Suoping, Wang Yu, Gao Huijiang, Gao Shenyang

Food and Machinery

To establish a rapid assay for Vibrio vulnificus (V. vulnificus) detection, a quantitative real-time PCR (qRT-PCR) method was developed targeting vvhA gene of V. vulnificus . The results showed that the tests for 15 strains of different bacteria by using this method, only the ones of V. vulnificus were positive, indicating high specificity of qRT-PCR. Moreover, the sensitivity of this specific method was 15 CFU/mL. Further stability and reproducibility test results showed that the coefficient of variation of Ct values of four duplicates were less than 2%. In addition, a total 2 positive samples for V. vulnificus were detected from …

A Pretargeted Approach For The Multimodal Pet/Nirf Imaging Of Colorectal Cancer, Pierre Adumeau, Kathryn E. Carnazza, Christian Brand, Sean D. Carlin, Thomas Reiner, Brian J. Agnew, Jason S. Lewis, Brian M. Zeglis

Publications and Research

The complementary nature of positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging makes the development of strategies for the multimodal PET/NIRF imaging of cancer a very enticing prospect. Indeed, in the context of colorectal cancer, a single multimodal PET/NIRF imaging agent could be used to stage the disease, identify candidates for surgical intervention, and facilitate the image-guided resection of the disease. While antibodies have proven to be highly effective vectors for the delivery of radioisotopes and fluorophores to malignant tissues, the use of radioimmunoconjugates labeled with long-lived nuclides such as 89Zr poses two important clinical complications: high radiation doses …

Transitions In Morphologies, Fluid Regimes, And Feeding Mechanisms During Development Of The Medusa Lychnorhiza Lucerna, Renato M. Nagata, André C. Morandini, Sean Colin, Alvaro E. Migotto, John H. Costello

Arts & Sciences Faculty Publications

The early ontogeny of scyphomedusae involves morphological and functional transitions in body plans that affect the predators' propulsive and feeding strategies. We applied highspeed videography digital particle image velocimetry (DPIV), and dye visualization techniques to evaluate alterations in swimming and feeding mechanisms during ontogeny of the rhizostome medusa Lychnorhiza lucerna Haeckel, 1880 (Scyphozoa, Rhizostomeae). During early ontogeny the ephyral mouth lips develop into complex filtering structures along the oral arms. The viscous environments (Reynolds number < 100) experienced by ephyrae constrain the feeding mechanisms that transport fluid during ephyral bell pulsations. In contrast, adult medusan fluid flows are dominated by inertial forces, and bell pulsations effectively transport fluids and entrained prey toward the oral arms. The oral arm surfaces are covered by motile epidermal cilia that drive these entrained flows through filtering gaps in the oral arms where food particles are retained. In addition to this process within the oral arms, vortices generated during bell pulsation flow downstream along the outside of the medusae and continuously transport prey toward the exterior oral arm surfaces. Although calanoid copepods are capable of escape velocities that greatly exceed L. lucerna's feeding current speeds, copepods often fail to detect the predator's feeding currents or inadvertently jump into medusan capture surfaces during failed escape attempts. Consequently, the comparatively weak predator feeding currents successfully capture a portion of the copepods encountered by swimming medusae. These results clarify the processes that enable rhizostome medusae to play key roles as consumers in tropical and subtropical coastal environments.

Effects Of Pan-Frying, Frying, Broiling On Eating Quality Of Hengshan Mutton, Li Linqiang, Gao Tianli, Zhang Lan, Liu Yongfeng

Food and Machinery

The objective of this study was to evaluate the effects of pan-frying, frying and broiling on eating quality of Hengshan mutton by regulating the cooking time and temperature. Texture analyzer was used to measure the texture profile analysis and shear force, meanwhile color measurement instrument was used to measure meat color. Results showed, for Pan-frying, Pan-fried mutton samples 2 min and 3 min treatments, the hardness, chewiness and resilience significantly were less than that of 4 min treatment (P<0.05), and the shear force was significantly less than 4 min treatment (P<0.05). With the extension of treatment time, the L^* value decreased gradually (P<0.05), processed for 2 min and 3 min, the a^* value of mutton sample was less than that of 4 min treatment (P<0.05), while b^*、c …

Studies On The Effects Of High-Voltage Electric Field On Hot-Air Drying Characteristics And Color Changing Of Apricot, Wang Qinghui, Yan Shengkun, Li Zhongxin, Abulizi Basiti

Food and Machinery

The drying characteristics and color changing experiments of apricot were investigated under different drying temperatures, air velocities and high-voltage electric field respectively. The results were as following. ① The drying time was shortened by increasing air temperatures or velocities. ② Weibull function could simulate the drying curves of apricot. The scale parameter (α) related with the temperature and air velocity and decreased with the increasing of either drying temperature or velocity. The shape parameter (β) was depended on the drying method. ③ The moisture effective diffusivity coefficient was 1.88×10^-10～3.18×10^-10 m²/s, and the activation energy was …

A High Precious Gutter Oil Detector Based On Complementary Metal Oxide Semiconductor, Yang Jie, Huang Haishen, Li Yangjun

Food and Machinery

Since the different conductivity of gutter oil and edible oil normally result in the different resistivity per unit volume, a linear detection module based on CMOS (Complementary Metal Oxide Semiconductor) was designed, which shorted to ground combined with directly selection the resistor string to divide voltage makes more linear precision voltage divider. Under the action of counting pulse, the count resistor divider compares with the reference voltage to achieve different number of high level, which not only can distinguish gutter oil and edible oil, but also come to the mass fraction of edible oil, which incorporates gutter oil. In the …

Improvement And Application Of Et22 Pneumatic Separator, Zhang Liangbin, Luo Fuwei, Fan Mingdeng, Xu Dayong

Food and Machinery

To solve the problems of ET22 Pneumatic Separator for high-speed belt machine, which is easy to deviation and pneumatic separator chamber leakage of large amount of material, two methods were put forward. On the one hand, transformed the fixed type of retaining belt into a movable type to ensure side seal, avoided material into high speed belt conveyor main roller and the driven roller caused by high speed belt deviation, on the other hand in the pneumatic separator chamber installed wind guiding device, and the wind was guided to the end of halftone, through the wind on the halftone back …

Research On Minimalism And Style Characteristics Of Japanese Contemporary Food Packaging, Lin Haiyan

Food and Machinery

Japanese Contemporary food packaging is featured by strong national characteristics and modern minimalism, recognized by designers and consumers, which is worthy of reference and learning. Through probing into the origins and historical contexts of minimalism in Japanese food packaging and by virtue of specific cases, this paper is aimed at expounding the minimalism of contemporary Japanese food packaging and gaining some enlightenment about Chinese food packaging designs from the law of parsimony.

Changes In Nitrite Contents And Antioxidant Properties Of Cowpeas Processed By Different Cooking Methods During Refrigerated Storage, Liu Danwen, Huang Shirong, Ge Du, Liu Bin

Food and Machinery

Changes in nitrite contents and antioxidant properties of cowpeas processed by three cooking methods (boiling, steaming and frying) during 4 ℃ storage were investigated. The polyphenol content and antioxidant activity of extracts from different treated cowpeas were determined by Folin-Ciocalteu assay and TEAC assay, respectively. Results showed that the different cooked cowpeas had significantly higher nitrite contents than the raw cowpeas. The nitrite contents in all the samples increased at first and then decreased during storage, with the highest contents being obtained at 3 days of storage. The boiled cowpeas had significantly higher nitrite content than the other processed cowpeas …

Design And Energy-Saving Effect Analysis Of Intelligent Fresh-Keeping System Of Puree Draft Beer, Duan Lian, Han Jitian, Huang Huaqi, Cheng Xianyao, Song Bo, Guo Fangquan, Xu Zaixian

Food and Machinery

The design procedures for the intelligent fresh-keeping system of puree draft beer, including the cylinder body, refrigeration, gas supplying, and intelligent control units were present in this study. The system we designed here could greatly prolong the preservation time and ensure the quality of puree draft beer. The performance of the system was evaluated along with the analysis of energy-saving effect.

Optimization On Ultrasonic-Microwave Combined Extraction Of Gypenosides And Synergetic Effect Analysis, Cheng Yiqun, Zhang Fan, Zhou Shoubiao, He Rongrong, Ji Hangyan, Huang Yi, Ji Yali, Chen Chunhua

Food and Machinery

Optimization on ultrasonic-microwave combined extraction of gypenosides, and the analysis on the synergistic effect between ultrasonic and microwave, were studied. The optimal extraction condition of gypenosides was obtained by response surface methodology. Four factors effect on the yield of gypenosides such as microwave power, microwave processing time, extraction temperature and extraction time were investigated. The synergistic effect of ultrasonic and microwave was analyzed by the yield of gypenosides under different extraction conditions. The optimal extraction condition of gypenosides by ultrasonic-microwave was ultrasonic open (50 W), microwave power at 613 W, microwave processing for 145 s, extraction temperature at 62.0 ℃, …

Progress In Research Of Mycelial Morphology Control Of Filamentous Fungiin Submerged Fermentation, Jiang Xuewei, Li Hao, Yang Chen, Xu Yantao, Luo Xiaoming

Food and Machinery

Filamentous fungi are widely used in industrial fermentation. Its mycelial morphology is closely combined with objective product yield and fermented liquid viscosity, and it is one of the key factors in the fermentation process control. In this paper, the research progress was reviewed on the mycelia of filamentous fungi in the submerged fermentation,including its morphological characteristics, the analysis and characterization methods, the mechanism of mycelial pellet formation, and the influence of mycelial morphology control in fermentation. Therefore, the author indicated that quantitative description the growth of filamentous fungi was the foundation of precision control on fermentation process.

Electronic Nose Technology And Its Application On Tea, Pan Yucheng, Song Lili, Ye Naixing, Pan Yuhua

Food and Machinery

The basic principle and structure of the electronic nose was introduced in this paper. Moreover, the application of electronic nose in the quality evaluation of tea, the classification of varieties, the detection of components etc were also reviewed. Finally, it was summarized that the problems existed in the application of electronic nose in tea, and then the future development direction was prospected for its in-depth study.

Selective Anticancer Activity Of Hydroxyapatite/Chitosan-Poly(D,L)-Lactide-Co-Glycolide Particles Loaded With An Androstane-Based Cancer Inhibitor, Nenad Ignjatović, Katarina M. Penov-Gaši, Victoria M. Wu, Jovana J. Ajduković, Vesna V. Kojić, Dana Vasiljević-Radović, Maja Kuzmanović, Vuk Uskoković, Dragab Uskoković

Pharmacy Faculty Articles and Research

In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. ¹H NMR and ¹³C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the …

CañAda De La Carpenteria, Glo No. 243, Monterey County, Diseños And Associated Historical Documents.

Monterey County

Diseños - Hand-drawn Spanish-Mexican map demonstrating the physical boundaries of land granted to Mexican citizens by the Spanish or Mexican.

Expediente – Hand-written Spanish-Mexican land grant document issued to Mexican citizens in Alta California for land in Alta-California.

Work Progress Administration Abstract (WPA Abstract) – A summary document prepared in 1938 under the Work Progress Administration program detailing the history of a rancho beginning with the Spanish-American land through the U.S. patenting process.

Hoffman Notes -- Summary of information concerning claims made for the Rancho lands from the Appendix listing Table of Land Claims in the Reports of Land Cases …

U.S. Drought Monitor, September 27, 2016, Chris Fenimore

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for September 27, 2016 (9/27/16) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Buena Vista, Glo No. 274, Monterey County, Diseños And Associated Historical Documents.

Monterey County

Diseños - Hand-drawn Spanish-Mexican map demonstrating the physical boundaries of land granted to Mexican citizens by the Spanish or Mexican.

Expediente – Hand-written Spanish-Mexican land grant document issued to Mexican citizens in Alta California for land in Alta-California.

Work Progress Administration Abstract (WPA Abstract) – A summary document prepared in 1938 under the Work Progress Administration program detailing the history of a rancho beginning with the Spanish-American land through the U.S. patenting process.

Hoffman Notes -- Summary of information concerning claims made for the Rancho lands from the Appendix listing Table of Land Claims in the Reports of Land Cases …

CañAda De La Segunda, Glo No. 283, Monterey County, Diseños And Associated Historical Documents.

Monterey County

Diseños - Hand-drawn Spanish-Mexican map demonstrating the physical boundaries of land granted to Mexican citizens by the Spanish or Mexican.

Expediente – Hand-written Spanish-Mexican land grant document issued to Mexican citizens in Alta California for land in Alta-California.

Work Progress Administration Abstract (WPA Abstract) – A summary document prepared in 1938 under the Work Progress Administration program detailing the history of a rancho beginning with the Spanish-American land through the U.S. patenting process.

Hoffman Notes -- Summary of information concerning claims made for the Rancho lands from the Appendix listing Table of Land Claims in the Reports of Land Cases …

Characterization Of Chemical Profiles Produced By Opuntia Humifusa In Response To Herbivory And Exogenous Methyl Jasmonate In Comparison To Hairy Root Cultures, John Milton Kilmer

Student Theses and Dissertations

The South American Cactus Moth, Cactoblastis cactorum (Berg), an invasive insect herbivore, has similar feeding habits and life history as a native Florida cactus moth, Melitara prodenialis Walker; however, regional Opuntia species only defend against M. prodenialis. Extracts of C. cactorum host plants and M. prodenialis host plants were compared to methyl jasmonate (MeJA)-treated Opuntia humifusa and MeJA-treated O. humifusa hairy root cultures using high performance thin layer chromatography. MeJA and C. cactorum treatments led to a reduction in tissue concentrations of terpenoid or phenolic compounds; however, significant increases in seven putative terpenoid compounds were observed in hairy root culture …

Developing High-Content Screening To Assay Trans-Epithelial Transport Of Complex Proteins, Reid Allan Martin

Student Theses and Dissertations

Therapeutic proteins are becoming one of the fastest growing classes of drugs on the market. These large biologically-synthesized macromolecules are typically administered intravenously and are dependent upon cellular receptors and other active uptake mechanisms for delivery into tissues and cells. There is significant interest in developing cell-based assays to assess kinetics and mechanisms of endocytosis and transcytosis of large therapeutic proteins across various cell layers. The goal of this research was to develop a transwell “disease tissue on a chip” model to compare rates and efficiencies of different delivery systems for moving bioactive lysosomal enzymes across a human epithelial cell …

Motor Experience Reprograms Development Of A Genetically-Altered Bilateral Corticospinal Motor Circuit, Najet Serradj, John H. Martin

Publications and Research

Evidence suggests that motor experience plays a role in shaping development of the corticospinal system and voluntary motor control, which is a key motor function of the system. Here we used a mouse model with conditional forebrain deletion of the gene for EphA4 (Emx1-Cre:EphA4tm2Kldr), which regulates development of the laterality of corticospinal tract (CST). We combined study of Emx1-Cre:EphA4tm2Kldr with unilateral forelimb constraint during development to expand our understanding of experience-dependent CST development from both basic and translational perspectives. This mouse develops dense ipsilateral CST projections, a bilateral motor cortex motor representation, and bilateral motor phenotypes. Together these phenotypes can …

Higginbotham, Rudy (Fa 896), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid only for Folklife Archives Project 896. Project titled: “Collection Project of Traditional Farm Implements.” Project includes survey sheets with brief descriptions of non-mechanized farm implements in Russell County, Kentucky. Sheets include tool name, brief description, informant’s name, and a photo of the implement.

Bolsa Del Potrero Y Moro Cojo Or Canada De La Sagrada Familia, Glo No. 254 , Monterey County, Diseños And Associated Historical Documents.

Monterey County

Diseños - Hand-drawn Spanish-Mexican map demonstrating the physical boundaries of land granted to Mexican citizens by the Spanish or Mexican.

Expediente – Hand-written Spanish-Mexican land grant document issued to Mexican citizens in Alta California for land in Alta-California.

Work Progress Administration Abstract (WPA Abstract) – A summary document prepared in 1938 under the Work Progress Administration program detailing the history of a rancho beginning with the Spanish-American land through the U.S. patenting process.

Hoffman Notes -- Summary of information concerning claims made for the Rancho lands from the Appendix listing Table of Land Claims in the Reports of Land Cases …

Bolsa Nueva Y Moro Cojo, Glo No. 245, Monterey County, Diseños And Associated Historical Documents.

Monterey County

Diseños - Hand-drawn Spanish-Mexican map demonstrating the physical boundaries of land granted to Mexican citizens by the Spanish or Mexican.

Expediente – Hand-written Spanish-Mexican land grant document issued to Mexican citizens in Alta California for land in Alta-California.

Work Progress Administration Abstract (WPA Abstract) – A summary document prepared in 1938 under the Work Progress Administration program detailing the history of a rancho beginning with the Spanish-American land through the U.S. patenting process.

Hoffman Notes -- Summary of information concerning claims made for the Rancho lands from the Appendix listing Table of Land Claims in the Reports of Land Cases …

Dissection Of Molecular Assembly Dynamics By Tracking Orientation And Position Of Single Molecules In Live Cells, Shalin B. Mehta, Molly Mcquilken, Patrick J. La Riviere, Patricia Occhipinti, Amitabh Verma, Rudolf Oldenbourg, Amy Gladfelter, Tomomi Tani

Dartmouth Scholarship

Regulation of order, such as orientation and conformation, drives the function of most molecular assemblies in living cells but remains difficult to measure accurately through space and time. We built an instantaneous fluorescence polarization microscope, which simultaneously images position and orientation of fluorophores in living cells with single-molecule sensitivity and a time resolution of 100 ms. We developed image acquisition and analysis methods to track single particles that interact with higher-order assemblies of molecules. We tracked the fluctuations in position and orientation of molecules from the level of an ensemble of fluorophores down to single fluorophores. We tested our system …

Adopters And Non-Adopters Of Low-Cost Household Latrines: A Study Of Corbelled Pit Latrines In 15 Districts Of Malawi., Rochelle Holm, Mavuto Tembo, Dalo Njera, Victor Kasulo, Mphatso Malota, Willy Chipeta, Wales Singini, Joshua Mchenga

Faculty and Staff Scholarship

The Sustainable Development Goals will challenge low- and middle-income settings to look at new approaches for rural sanitation. In 2013, Mzuzu University, in partnership with United Nations Children's Fund (UNICEF) Malawi, started a low-cost latrine program in rural areas using the corbelled latrine design supported by locally owned sustainable businesses. The objective of this work was to trace customers (early household adopters) and non-customers through field observations and interviews in 15 districts of Malawi. The research team spent 193 personnel work days in data collection and found 21 households as adopters in 7 districts. Most respondents had a preference with …

Fhf-Independent Conduction Of Action Potentials Along The Leak-Resistant Cerebellar Granule Cell Axon, Katarzyna Dover, Christopher Marra, Sergio Solinas, Marko Popovic, Sathyaa Subramaniyam, Dejan Zecevic, Egidio D'Angelo, Mitchell Goldfarb

Publications and Research

Neurons in vertebrate central nervous systems initiate and conduct sodium action potentials in distinct subcellular compartments that differ architecturally and electrically. Here, we report several unanticipated passive and active properties of the cerebellar granule cell’s unmyelinated axon. Whereas spike initiation at the axon initial segment relies on sodium channel (Nav)-associated fibroblast growth factor homologous factor (FHF) proteins to delay Nav inactivation, distal axonal Navs show little FHF association or FHF requirement for high-frequency transmission, velocity and waveforms of conducting action potentials. In addition, leak conductance density along the distal axon is estimated as o1% that of somatodendritic membrane. The faster …

Description Of New Ceratitis Species (Diptera: Tephritidae) From Africa, Or How Morphological And Dna Data Are Complementary In Discovering Unknown Species And Matching Sexes, Marc De Meyer, Maulid Mwatawala, Robert S. Copeland, Massimiliano Virgilio

All Peer-Reviewed Publications

This paper describes five new Ceratitis species from the eastern and southern parts of the Afrotropical Region: C. (Pterandrus) quilicii De Meyer, Mwatawala & Virgilio sp. nov.; C. (Ceratalaspis) pallidula De Meyer, Mwatawala & Virgilio sp. nov.; C. (Ceratalaspis) taitaensis De Meyer & Copeland sp. nov.; C. (Ceratalaspis) sawahilensis De Meyer & Virgilio sp. nov.; and C. (Ceratalaspis) flavipennata De Meyer & Virgilio sp. nov. Their relationships with closely allied species within their respective subgenera are discussed where appropriate, and diagnostic characters are given. DNA barcodes are provided for all new species. In addition, the hitherto unknown male of C. …

Different Roads Lead To Rome: Integrative Taxonomic Approaches Lead To The Discovery Of Two New Lizard Lineages In The Liolaemus Montanus Group (Squamata: Liolaemidae), Cesar Aguilar, Perry L. Wood Jr., Mark C. Belk, Mike H. Duff, Jack W. Sites Jr.

Faculty Publications

Integrative taxonomy (IT) is becoming a preferred approach to delimiting species boundaries by including different empirical criteria. IT methods can be divided into two types of procedures both of which use multiple kinds of evidence: step-by-step approaches test hypotheses by sequential evaluation in a hypothetic-deductive framework, while model-based procedures delimit groups based on statistical information criteria. In this study we used a step-by-step approach and a Gaussian clustering (GC) method to test species boundaries in the northernmost species of the Liolaemus montanus group. We used different methods based on mitochondrial and nuclear DNA sequence data, morphological measures and niche envelope …

Structural Dichotomy Of The Mind; The Role Of Sexual Neuromodulators, Ion G. Motofei, David L. Rowland

Journal of Mind and Medical Sciences

The mind (mental function) and sexuality represent two distinct environmental functions, but which are supported within the brain by a common (somatic-autonomic) neurobiological substrate. As a consequence, mental function takes on autonomic characteristics from the sexual-autonomic system (like autonomy, duality), while sexual function takes on features from mental functioning (such as lateralization). In this paper we discuss the lateralized action of two classes of sexual neuromodulators: hormones and pheromones. This process of lateralization is assimilated with the structural dichotomy of the mind.

A relatively similar process but related to informational dichotomy of the mind will be presented in a forthcoming …