Life Sciences Commons^™

A Cryab Interactome Reveals Clientele Specificity And Dysfunction Of Mutants Associated With Human Disease, Whitney Katherine Hoopes

Theses and Dissertations

Small Heat Shock Proteins (sHSP) are critical molecular chaperones that function to maintain protein homeostasis (proteostasis) and prevent the aggregation of other proteins during cellular stress. Any disruption in the process of proteostasis can lead to prevalent diseases ranging from cancer and cataract to cardiovascular and Alzheimer's disease. CryAB (αB-crystallin, HspB5) is one of ten known human sHSP that is abundant in the lens, skeletal, and cardiac muscle. This protein is required for cardiac function and muscle cell integrity. When the cell experiences physiological stress, including heat shock, CryAB moves to the cytoskeleton to act as a chaperone and prevent …

The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin

School of Biological Sciences: Dissertations, Theses, and Student Research

Due to the increased number of immunocompromised patients, infections by Candida albicans have significantly increased in recent years. C. albicans transition from yeast to germ tubes is an essential factor for virulence. In this study we noted that Lee's medium, commonly used to induce filamentation, contained 500-fold more biotin than needed for growth. Thus, we investigated the effects of excess biotin on growth rate and filamentation by C. albicans in different media. At 37 °C, excess biotin (4 µM) enhanced germ tube formation (GTF) ca. 10-fold in both Lee's medium and a defined glucose proline medium, and ca. …

Inter- And Intra-Individual Variation In Predator-Related Behavioral Plasticity Expressed By Female Green Swordtails (Xiphophorus Hellerii), Rachael A. Disciullo

School of Biological Sciences: Dissertations, Theses, and Student Research

Phenotypic plasticity is the ability of one genotype to express multiple phenotypes under variable environments. Behavioral plasticity is a type of phenotypic plasticity in which individuals adjust behavior in response to changes in environment. Often, behavioral plasticity is studied at the level of the population, rather than at the level of the individual. Further, few studies have considered the effect of individual traits, such as size and age, on the expression of behavioral plasticity, or, how individual plasticity may be correlated across different contexts. In this study, we used female green swordtails (Xiphophorus hellerii) to test the effects …

Microplastic In Surface Waters Of Urban Rivers: Concentration, Sources, And Associated Bacterial Assemblages, Amanda Rae Mccormick, Timothy Hoellein, Maxwell G. London, Joshua Hittie, John J. Kelly, John W. Scott

Biology: Faculty Publications and Other Works

The ecological dynamics of microplastic (<5 mm) are well documented in marine ecosystems, but the sources, abundance, and ecological role of microplastic in rivers are unknown and likely to be substantial. Microplastic fibers (e.g., synthetic fabrics) and pellets (e.g., abrasives in personal care products) are abundant in wastewater treatment plant (WWTP) effluent, and can serve as a point source of microplastic in rivers. The buoyancy, hydrophobic surface, and long transport distance of microplastic make it a novel substrate for the selection and dispersal of unique microbial assemblages. We measured microplastic concentration and bacterial assemblage composition on microplastic and natural surfaces upstream and downstream of WWTP effluent sites at nine rivers in Illinois, United States. Microplastic concentration was higher downstream of WWTP effluent outfall sites in all but two rivers. Pellets, fibers, and fragments were the dominant microplastic types, and polymers were identified as polypropylene, polyethylene, and polystyrene. Mean microplastic flux was 1,338,757 pieces per day, although the flux was highly variable among nine sites (min = 15,520 per day, max = 4,721,709 per day). High-throughput sequencing of 16S rRNA genes showed bacterial assemblage composition was significantly different among microplastic, seston, and water column substrates. Microplastic bacterial assemblages had lower taxon richness, diversity, and evenness than those on other substrates, and microplastic selected for taxa that may degrade plastic polymers (e.g., Pseudomonas) and those representing common human intestinal pathogens (e.g., Arcobacter). Effluent from WWTPs in rivers is an important component of the global plastic “life cycle,” and microplastic serves as a novel substrate that selects and transports distinct bacterial assemblages in urban rivers. Rates of microplastic deposition, consumption by stream biota, and the metabolic capacity of microplastic biofilms in rivers are unknown and merit further research.

Draft Genome Sequence For A Urinary Isolate Of Nosocomiicoccus Ampullae, Catherine Putonti, Evann E. Hilt, Travis K. Price, Katherine Diebel, Alan J. Wolfe

Biology: Faculty Publications and Other Works

A draft genome sequence for a urinary isolate of Nosocomiicoccus ampullae (UMB0853) was investigated. The size of the genome was 1,578,043 bp, with an observed G+C content of 36.1%. Annotation revealed 10 rRNA sequences, 40 tRNA genes, and 1,532 protein-coding sequences. Genome coverage was 727× and consisted of 32 contigs, with an N₅₀ of 109,831 bp.

Arkansas Corn And Grain Sorghum Peformance Tests 2016, R. D. Bond, J. A. Still, D. G. Dombek

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state, and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

Genetic And Biochemical Analysis Of The Micrococcin Biosynthetic Pathway, Philip Ross Bennallack

Theses and Dissertations

Declining antibiotic discovery and flourishing antibiotic resistance have led to a modern antibiotic crisis which threatens to compromise our ability to treat infectious disease. Consequently, there is significant interest in developing new antibiotics with novel modes of action and chemical properties. Ribosomally synthesized and post-translationally modified peptides (RiPPs) are natural compounds with the appealing attributes of being derived directly from a genetic template while possessing numerous exotic chemical features that contribute to stability and antimicrobial activity. Abundant in nature, their diverse range of biological activities makes them excellent prospects for antibiotic development. Thiopeptides, a RiPP family rich in chemical complexity, …

Spatial And Temporal Spread Of Maize Stem Borer Busseola Fusca (Fuller) (Lepidoptera: Noctuidae) Damage In Smallholder Farms, Frank T. Ndjomatchoua, Henri E.Z. Tonnang, Christophe Plantamp, Pascal Campagne, Clément Tchawoua, Bruno P. Le Ru

All Peer-Reviewed Publications

The main purpose of this study was to understand the spatio-temporal spread of the maize stem borer Busseola fusca (Fuller) (Lepidoptera: Noctuidae) in smallholder maize farms. The analysis carried out allowed the establishment of complementary sampling scheme and analysis that can be applied to investigate the propagation of stem borer damages and extended to other insect pests. This approach requires consideration of all plants point locations, the knowledge on the level of damage and its characterization. Results showed that there was a two-week interval between occurrence of the peaks of leaf damage and male adult moth abundance. The prior role …

Neurobehavioral Markers Of Resilience To Depression Amongst Adolescent Exposed To Child Abuse, Meg J. Dennison, Margaret A. Sheridan, Daniel S. Busso, Jessica L. Jenness, Matthew Peverill, Maya L. Rosen, Katie A. Mclaughlin

Neuroscience: Faculty Publications

Childhood maltreatment is strongly associated with depression, which is characterized by reduced reactivity to reward. Identifying factors that mitigate risk for depression in maltreated children is important for understanding etiological links between maltreatment and depression as well as improving early intervention and prevention. We examine whether high reward reactivity at behavioral and neurobiological levels is a marker of resilience to depressive symptomology in adolescence following childhood maltreatment. A sample of 59 adolescents (21 with a history of maltreatment; Mean Age = 16.95 years, SD = 1.44) completed an fMRI task involving passive viewing of emotional stimuli. BOLD signal changes to …

Program And Abstracts Of The 63rd Annual Conference Of The Mississippi Entomological Association, October 17-18, 2016, Mississippi Entomological Association

Midsouth Entomologist

No abstract provided.

Productivity, Profitability And Partial Nutrient Balance In Maize-Based Conventional And Organic Farming Systems In Kenya, Noah Adamtey, Martha W. Musyoka, Christine Zundel, Juan Guillermo Cobo, Edward Karanja, Komi K.M. Fiaboe, Anne Muriuki, Monicah Mucheru-Muna, Bernard Vanlauwe, Estelle Berset, Monika M. Messmer, Andreas Gattinger, Gurbir S. Bhullar, Georg Cadisch, Andreas Fliessbach, Paul Mäder, Urs Niggli, Dionys Foster

All Peer-Reviewed Publications

In 2007 two long-term trials were established in Kenya to contribute research-based evidence to the global debate on the productivity, economic viability and sustainability of different agricultural production systems. These trials compare conventional (Conv) and organic (Org) farming systems at high and low input levels at two locations, i.e. Chuka, with Humic Nitisols, high inherent soil fertility and rainfall, and Thika, with Rhodic Nitisols with low soil fertility and rainfall. The high input systems (High) represent commercial-scale, export-oriented production that uses the recommended amounts of fertilisers, pesticides and irrigation water to generate high yields, whilst the low input systems (Low) …

National Smokejumper Assocation Trail Maintenance Annual Report For 2016, National Smokejumper Association Trails Committee

Trails Reports

The Trails Maintenance Program Annual Reports compiled by the National Smokejumper Association Trails Committee summarizes the activities of the Trails Program. The members of the program also go by the title Trails Restoration and Maintenance Project Specialists (TRAMPS). The contents of the 2011 report include: Volunteer of the Year; Program summary; Tax Exemption note; Editor's Note; Base abbreviations; Bear Creek Ranger Station; Bear Creek Trails; Bonners Ferry Trails & Fence; Hondo Cabin; Horse Creek Trails Maintenance; Horse Camp; Lewis & Clark; Mann Gulch (two tales); Rio Medio; Snyder Guard Station; Rocky Mtn. NP; East Fork Blacktail Cr. Bridge; BWCAW; Pretty …

Csp And “Omics” Technology Applied On Versatile And Intelligent Portable Platform For Modeling Complex Bio-Medical Data, Florentin Smarandache, Luige Vladareanu, Mihaiela Iliescu, Hongbo Wang, Feng Yongfei, Victor Vladareanu

Branch Mathematics and Statistics Faculty and Staff Publications

This paper presents relevant aspects of the idea of using the digital medicine in cancer, so that to shape a viable strategy for creating and implementing an interactive digital platform, NEO-VIP, that should be the basic support to design the strategy for integration of basic, clinical and environmental research on neoplasia progression to cancer. The two main components of the VIPRO Platform are represented by the workstation “Engineering Station” for CPS (Cyber Physical System) and “omics” technology and by the “Graphical Station” for the development of a virtual mechatronic system environment and virtual reality for system components' motion. The NEO-VIP …

The Biological Function Of Hammerhead Rana Ribozyme In Human Genome, Ibtehal M. A. Awad

Theses

Hammerhead RNA [HHR] sequence originally found in viroids is also detected in many species. HHR are often found in introns of different genes ranging from prokaryotes to

eukaryotes. HHR can form specific tertiary structure that make specific region susceptible to cleavage. However, the role of the HHR is not clear in other life forms. This study aims to

Investigate the role of HHR in the intron 6 of (RECK) gene. Our study shows that when blocking the HHR sequence in the intron6, the production of RECK protein declines which may be due to the effect of HHR in splicing and …

Microarray Analysis Of Arabidopsis Wrky33 Mutants In Response To The Necrotrophic Fungus Botrytis Cinerea, Shamma Saeed Salem Al Shamsi

Theses

Plants are exposed to many environmental stresses that affect their growth and development. These stresses include biotic stresses (via organisms) and abiotic stresses (via environment). Plants respond to these stresses by transcriptional reprogramming and different signaling pathways. Arabidopsis thaliana has shown great sensitivity to the biotic stress Botrytis cinerea. The WRKY33 gene plays an important role in plant defense mechanism against this pathogen. The overall goal is to identify common regulated genes of wrky33 mutant and 35S:WRKY33 over expressing transgenic lines in response to B. cinerea; ultimately to improve plant stress tolerance in Arabidopsis. The specific aims are to: (1) …

The Effects Of Elevated Pco₂ And Temperature On The Embryonic Development, Larval Survivorship, Condition, Calcification, Morphology, And Behavior Of The Florida Stone Crab, Menippe Mercenaria, Philip Michael Gravinese

Theses and Dissertations

The emission of greenhouse gases into the atmosphere over the last century has increased atmospheric and oceanic temperatures, and has led to a decrease in oceanic pH. The increased ocean temperatures and reduced pH have been detrimental to marine life. In addition, Florida’s watersheds have suffered from decades of disrupted hydrology and diverted runoff, which has increased nutrients and changed coastal carbonate chemistry. Although the adult stages of many marine species are capable of tolerating fluctuations in environmental conditions (i.e., elevated pCO₂ tolerances to such conditions improve. and temperature), marine larvae that hatch in coastal habitats may not have the …

Cho Endonuclease Functions During Dna Interstrand Crosslink Repair In Escherichia Coli, Anthonige Vidya Perera, James Brian Mendenhall, Charmain T. Courcelle, Justin Courcelle

Biology Faculty Publications and Presentations

DNA interstrand crosslinks are complex lesions that covalently link both strands of the duplex DNA. Lesion removal is proposed to initiate via the UvrABC nucleotide excision repair complex, however less is known about the subsequent steps of this complex repair pathway. In this study, we characterized the contribution of nucleotide excision repair mutants to survival in the presence of psoralen-induced damage. Unexpectedly, we observed that the nucleotide excision repair mutants exhibit differential sensitivity to psoralen-induced damage, with uvrC mutants being less sensitive than either uvrA or uvrB. We show that Cho, an alternative endonuclease, acts with UvrAB and is …

The Prevalence Of Streptococcus Pyogenes And Its Emm Gene Types Among School Children In Ai Ain, Uae, Fatima Hammad Ali Al Shamisi

Theses

Streptococcus pyogenes, also known as Group a streptococcus (GAS), causes a wide variety of diseases in children and adults, and it frequently infects the pharynx and skin; pharyngitis and tonsillitis are one of the commonest diseases caused by this pathogen. GAS evaded by many virulence factors, however, the most significant part is the M protein, which plays a major role in its pathogenicity. Over 225 M protein types have been identified so far by the emm typing technique. Identification and emm typing of GAS is an essential part of epidemiological and pathogenic studies of streptococcal diseases, as different regions of …

Muscle Fatigue From The Perspective Of A Single Crossbridge, Edward Patrick Debold, Robert H. Fitts, Christopher W. Sundberg, Thomas M. Nosek

Biological Sciences Faculty Research and Publications

The repeated intense stimulation of skeletal muscle rapidly decreases its force- and motion-generating capacity. This type of fatigue can be temporally correlated with the accumulation of metabolic by-products, including phosphate (P_i) and protons (H⁺). Experiments on skinned single muscle fibers demonstrate that elevated concentrations of these ions can reduce maximal isometric force, unloaded shortening velocity, and peak power, providing strong evidence for a causative role in the fatigue process. This seems to be due, in part, to their direct effect on muscle’s molecular motor, myosin, because in assays using isolated proteins, these ions directly inhibit myosin’s …

The Role Of Acidosis In Fatigue: Pro Perspective, Robert H. Fitts

Biological Sciences Faculty Research and Publications

No abstract provided.

Acoustic Evidence Of Bats Using Rock Crevices In Winter: A Call For More Research On Winter Roosts In North America, Cliff A. Lemen, Patricia W. Freeman, Jeremy A. White

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Northern Long-eared Myotis (Myotis septentrionalis) is known to hibernate in mines and caves, often using cracks within these hibernacula as roost sites. We hypothesized that M. septentrionalis might use deep cracks in rock outcrops for hibernation as well. To test this hypothesis, we placed acoustical bat detectors near rock outcrops away from any known mines or caves during winter in Nebraska. We documented calls of M. septentrionalis as well as Perimyotis subflavus and Eptesicus fuscus in December near rock outcrops, which suggests that individuals of all three species were hibernating in rock crevices in winter. Of the …

Guidelines To Avoid Typical Difficulties According To The Rubric For Experimental Design (Red), Annwesa Dasgupta, Nancy Pelaez

PIBERG Publications

Experimental design is an important component of undergraduate biology education as it generates knowledge of biology. Despite its importance, there is limited information about what students actually learn from designing experiments. Dasgupta et al (2014) reported on the development and validation of a Rubric for Experimental Design (RED), informed by a literature review and empirical analysis of thousands of undergraduate biology students’ responses to three published assessments. The RED is a useful probe for five major areas of experimental design abilities: the variable properties of an experimental subject; the manipulated variables; measurement of outcomes; accounting for variability; and the scope …

Considerations For Sage-Grouse Management Objectives, David K. Dahlgren, Terry A. Messmer, Eric T. Thacker

All Current Publications

This fact sheet describes ways to conserve and manage sage-grouse and includes information on population dynamics; movements, habitat, and climatic interactions; and management objectives.

Mapping Landscape Values Using Social Media, Lael Gilbert, Jordan W. Smith, Boris Van Zanten, Derek Van Berkel

All Current Publications

Social media data are providing scientists with a variety of new ways to examine how and why individuals value particular natural landscapes. In this fact sheet, we review cutting edge research that used millions of photos posted to Instagram, Flickr and Panaramio to examine which European landscapes individuals value most. The research is the first of its kind to use social media data to identify the public’s most valued landscapes across an entire continent. The research is also the first to compare the spatial agreement between geotagged imagery uploaded to different platforms.