Life Sciences Commons^™

Spatial Variaiton In Host-Associated Microbiota, Daniel Malagon

All Dissertations

Many organisms rely upon the internal and/or external microbial communities they harbor. Microbial communities which depend upon other organisms for survival are known as host-associated (HA) microbial communities. HA microbial communities differ in diversity and composition from surrounding environmental microbiota and are generally less diverse than surrounding soil microbiomes. Although distinct bacterial profiles exist between host and environment, microbial scale dispersal often links the two. For this reason, host and environmental microbial communities can be correlated (e.g., dominant taxa in HA microbiota are dominant in the host microenvironment or vice versa). Eukaryotic hosts act as islands for HA microbiota. Unlike …

Investigate The Use Of Nucleic Acids As Pesticides To Manage Major Pests And Pathogens Of Crops In South Carolina, Prasanna Valavanur Shekar

All Dissertations

The sugarcane aphid (SCA, Melanaphis sorghi), first discovered on grain sorghum in late 2013, has gradually become a major insect pest of sorghum. It inflicts significant damage to the crop year after year, causing millions of dollars in losses throughout the sorghum-producing regions of the United States. Similarly, Armillaria root rot—also referred to as oak root rot—is a major cause of decline in peach (Prunus persica) production in the southeastern United States. This disease is caused by Armillaria mellea and Armillaria tabescens. Current management practices for controlling M. sorghi include the use of synthetic pesticides, resistant varieties, and regular scouting. …

Amyloid Aggregation And Mitigation, Zhenzhen Zhang

All Dissertations

The buildup of protein in tissues and organs plays a major role in unfunctional several serious diseases, including Alzheimer’s disease, Parkinson’s disease, and Type 2 diabetes, each tied to specific proteins that form harmful amyloid deposits. Amyloid aggregation typically follows a sigmoidal kinetic curve, beginning with a slow lag phase, followed by rapid fibril growth, and eventually reaching a plateau where mature, stable fibrils are formed. However, the early stage of aggregation remains particularly difficult to capture experimentally due to the highly dynamic nature and low abundance of small oligomeric species. In this dissertation, we employed discrete molecular dynamics (DMD) …

The Wellness Connection May 2025, Roger Williams University School Of Law

Life of the Law School (1993- )

No abstract provided.

Impact Of Invasive Pine Trees And Their Ectomycorrhizal Fungi Symbionts Upon The Properties And Functions Of Soils In Southern Hemisphere Eucalyptus Forests, Jack T. Baker

Honors Theses

P. radiata trees are invading Southern Hemisphere Eucalyptus forests within New Zealand and southeastern Australia, resulting in a novel codominant forest system. This codominant invasion is unique relative to other pine invasions throughout the Southern Hemisphere. Ectomycorrhizal fungi (EMF) play an important role in all pine invasions and are found within each instance of invasion. The impact of ectomycorrhizal fungi and Pinus trees upon the properties of the soil within Eucalyptus forests is not very well studied nor understood. Our study sought to provide insight into the implications of this invasion upon the soil, and consequently the ecosystem as …

Stereotypic Behaviors And Physiological Indicators Of Stress Occur In Horses With Restricted Durations Of Turnout, Elizabeth E. Rodgers

Department of Animal Science: Dissertations, Theses, and Student Research

The confinement of horses to stalls with limited or no turnout time restricts their natural roaming behaviors, which potentially increases stress and behavioral stereotypies. This study investigated the impact of turnout duration on behavioral and physiological stress indicators in healthy horses. Adult horses (n = 11) were enrolled in a 3 treatment (0-, 15-, and 60-minute turnout/day) Latin square design, including 3 12 d experimental periods over 6 weeks. Horses were acclimated to turnout (24.38 x 64.01m indoor arena) and stalls (2.74 x 4.87m - 3.66 x 3.66m) stalls prior to data collection on day 1. Experienced handlers managed …

Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle

Department of Animal Science: Dissertations, Theses, and Student Research

Snakes in human care often lack diversity in the diet. Rodents account for the majority of meals, while wild snakes are consuming a varied diet composed of multiple different whole prey species. By feeding only one species of prey, the chance of nutrient deficiencies and other health concerns increases. To promote variety in the managed diet, two commercially available whole prey species (mice (Mus musculus) and chicks (Gallus spp.)) and one novel species (Seba’s short-tailed bats (Carollia perspicillata)), were analyzed for their dry matter (DM), organic matter (OM), crude protein (CP), crude fat …

Evaluation Of Two Different Sweet Bran Products On Beef Cattle Digestion Characteristics And A Review Of Ramp As An Alternative Step-Up Program, Emily K. Hoefling

Department of Animal Science: Dissertations, Theses, and Student Research

Two Sweet Bran products were evaluated at 13%, 18%, and 23% inclusion of the diet on total tract digestibility and rumen fermentation parameters. Seven ruminally cannulated, heifers were used in a 6 x 7 row-column design with 21-d periods consisting of 16-d adaptation period followed by a 5-d collection. Dietary treatments were designed in a 2 x 3 factorial arrangement with 6 periods. Factors included two Sweet Bran products that differed in particle size (Sweet Bran-SP; GMD = 1.79 mm or Sweet Bran-LP; GMD = 2.51 mm) and three dietary inclusion (13%, 18%, or 23% of diet dry matter). The …

Methane Mitigation And Its Effects On Energy Utilization In Lactating Dairy Cattle, Shan C. Sherwood

Department of Animal Science: Dissertations, Theses, and Student Research

Improved methane (CH₄) mitigation strategies are needed to achieve the greenhouse gas emission goals of the dairy industry. The first experiment was conducted to evaluate the effects of feeding black cumin (Nigella sativa) seed oil and acetate (BCA) on CH₄ production and whole-animal energy utilization. Twelve dry Jersey cows were used in a 2-period crossover design study with 28d periods. The BCA treatment was compared to a control diet with corn oil. Both BCA and corn oil were added to the base diet as a top dress. Results from this study did not show any …

Small Cereal Grain Silage Producer Practices And Key Factors To Consider For Operations, Alexa R. Simons

Department of Animal Science: Dissertations, Theses, and Student Research

The use of small grain silage to feed livestock has grown in popularity, although the more common source of silage is annual corn. A benefit of producing small cereal grain is double cropping, harvest a forage source in the spring prior to planting the summer annual crop. Although the cereal and corn silage processes are similar there are key differences within management decisions for producers. Objective one was to understand current management decisions from Nebraska producers pre- and post- fermentation surveys along with small cereal silage samples were analyzed. Some best management practices such as the dry matter (DM), DM …

Precision Feeding Methods To Increase Nitrogen And Energy Utilization In Lactating Dairy Cows, Chloe Emily Moss Harris

Department of Animal Science: Dissertations, Theses, and Student Research

The environmental and economic costs of overfeeding N to dairy cattle are a challenge in today’s dairy industry. Two studies were conducted to address new opportunities for precision feeding N, which is a strategy where producers strive to feed cows the precise amount of N and amino acids that meet their individual requirements.

The objective of the first study was to evaluate the effects of supplementing a rumen protected methionine (RPM) product via automatic milking system (AMS) concentrate on milk protein yield in lactating dairy cows. Fifty-one cows were randomly assigned to control (no RPM) or treatment groups. Milk yield …

Passive Dna Transfer To Fidget Blasters Stored In Cars, Rachel Chan

Student Theses

This study evaluated the amount of detectable DNA accumulated on clean plastic fidget blasters, used to simulate firearms, over a seven-day storage period inside a vehicle. Twelve volunteers were asked to place one fidget blaster each in the glove box and underneath the driver’s seat from behind. After the seven-day period, the volunteers returned the fidget blasters, donated a reference sample, and filled out a questionnaire. The grip, slide, and trigger were swabbed for DNA and extracted. The extracts were quantified to determine the concentration of human DNA, and if the samples met the PCR threshold to be genotyped. The …

Melanaphis Sacchari/Sorghi Complex: Current Status, Challenges And Integrated Strategies For Managing The Invasive Sap-Feeding Insect Pest Of Sorghum, Alejandro Vasquez, Joseph Belsky, Neetu Khanal, Heena Puri, Devi Balakrishnan, Neelendra K. Joshi, Joe Louis, Glenn Studebaker, Rupesh Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Melanaphis sacchari (Zehntner;Hemiptera: Aphididae), sugarcane aphid (SCA), is an invasive phloem-feeder found worldwide with a wide host range of economically important plants including sorghum and sugarcane. Given its high reproductive capacity and ability to rapidly spread over long distances, SCA presents challenges for effective control, leading to substantial economic losses. Recent studies have identified two multiloci SCA genotypes specialized in feeding on sugarcane (MLL-D) and sorghum (MLL-F) in the USA, which raises concerns as the USA is the second largest sorghum-producing country. This has encouraged research towards identifying these two biotypes where some research has stated them as two species; …

Investigating The Effects Of Hepatocyte Growth Factor On Primary Myoblast Proliferation, Grace Heller

Biomedical Engineering

Peripheral artery occlusive disease (PAOD) is a progressive vascular condition that affects over 200 million people worldwide and approximately 8 million in the United States. It is characterized by atherosclerotic narrowing of peripheral arteries, leading to reduced blood flow, ischemia, and, in severe cases, tissue loss and limb amputation. Although surgical revascularization is the gold standard, many patients—particularly those with comorbidities like diabetes— continue to experience poor outcomes, emphasizing the need for alternative therapies. One promising strategy is to enhance collateral arteriogenesis, the remodeling and enlargement of collateral arterioles that naturally bypass occluded vessels. Satellite cells (SCs), which give rise …

Variation In Stress Response Regulation Across Natural Isolates Of Saccharomyces Cerevisiae, Crystal Christine Crook

Graduate Theses and Dissertations

The stress response of Saccharomyces cerevisiae is a valuable area of study for understanding cellular adaptation mechanisms that govern survival in fluctuating environments. This dissertation investigates stress response regulation in yeast through the roles of Hog1 mitogen-activated protein kinase (MAPK) and the post-transcriptional regulator Mkt1, a key post-transcriptional regulator, in diverse wild yeast isolates. Hog1 is traditionally known for its central role in osmotic stress regulation in the high-osmolarity glycerol (HOG) pathway. This study expands the functional scope of Hog1 to include oxidative and ethanol-induced stress responses.

In parallel, we investigate post-transcriptional regulation of stress responses by the Mkt1 interactome, …

Crispr-Cas9 Integration Of Salmon Elovl2 In Zebrafish Enhances Lc-Pufa Biosynthesis And Growth Across Diets, Waleed Sami J. Al-Bachry

Graduate Theses and Dissertations

Fish are a rich source of EPA and DHA, which are well-known long-chain polyunsaturated fatty acids (LC-PUFAs). The sustainable production of these essential fatty acids poses significant challenges, mainly due to our dependence on marine fish and certain nuts. CRISPR-Cas system was utilized to insert the elongation of long-chain fatty acids-like2 (Elovl2) gene from Salmo salar into Danio rerio. Elovl2 was introduced at 10-15-minute post-fertilization, and successful integration confirmed by PCR genotyping, sequencing and expression of EGFP. Approximately 67% of injected embryos successfully exhibited EGFP and sequence results verified the gene integration of Salmo salar Elovl2 at the first start …

Wired For Degradation: How Post-Translational Control Of Inducible Camp Early Repressor Protein Influences Melanoma Fate, Melissa Rose Spigelman

Theses, Dissertations and Culminating Projects

Melanoma is a highly aggressive form of skin cancer frequently driven by mutations in the BRAF gene, particularly the BRAFⱽ⁶⁰⁰ᴱ variant, which leads to sustained activation of the MAPK signaling cascade. Recent studies implicate the cyclic AMP (cAMP) signaling axis in melanoma progression and therapeutic resistance, highlighting the role of the Inducible cAMP Early Repressor (ICER), a transcriptional antagonist of CREB and CREM. ICER is downregulated during melanomagenesis and subject to post-translational regulation via phosphorylation and ubiquitin-mediated degradation. To investigate ICER’s functional relevance in vivo, e utilized a zebrafish melanoma model harboring brafⱽ⁶⁰⁰ᴱ, tp53−/−, and mitf−/−mutations, in which mitf expression …

Understanding Pre-Harvest Hedging Behavior And Basis Forecasting: The Role Of Information In Grain Marketing, Iyore Eronmwon

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation examines decision-making under risk in grain marketing, focusing on how information influences behavior, attitudes toward accessible information, and methods for forecasting commodity basis. Through three essays, it explores the impact of information on hedging decisions, the determinants of information-seeking behavior, the extent to which students can serve as proxies for farmers in risk experiments, and the effectiveness of different forecasting approaches for commodity basis.

The first essay examines the impact of information on pre-harvest grain marketing hedging decisions and grain marketing information-seeking behavior. Specifically, it evaluates two types of information hypothesized to influence decision-making: expected cost of production …

Considerations And Techniques For Producing Urban Tree Canopy Maps Using Freely Available And Accessible Methods, Hugh Reed Ellerman

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Urban forests are valued as green infrastructure for the variety of benefits they provide across ecological, social, public health, and economic domains. To understand the distribution of trees, maps are required. Many methods of mapping tree cover use expensive and data-intensive datasets such as hyperspectral and LiDAR imagery, making these methods inaccessible to municipal forest managers. Variations in the modelling approaches of existing, freely available methods are not addressed in sufficient enough detail to understand the trade-offs implicit in these variations. Further, characteristics of urban tree canopy mapping (high spatial resolution imagery, heterogeneous urban environments, the importance of the tree …

Gonadotropin-Releasing Hormone Ii And Its Receptor: Novel Regulators Of Semen Quality And Follicular Function In Swine, Caitlin Elizabeth Ross

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Pigs are the only mammalian livestock species encoding functional proteins for the second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II). In the first study, GnRHR-II localized to developing germ cells and the connecting piece of ejaculated spermatozoa. Concurrently, GnRH-II was most abundantly produced in the testis compared with other reproductive tract organs and was present within seminal plasma. Moreover, treatment of extended semen with GnRHR-II analogues altered sperm motility, morphology, and kinematics. For remaining studies, we utilized a swine line with ubiquitous knockdown of GnRHR-II (GnRHR-II KD) developed in our laboratory. The second study demonstrated that circulating GnRH-II …

Interferon Regulatory Factor 3 In Interferon Gamma Receptor Signaling And Cancer Immunology, Zachary P. Guinn

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Every year millions of people die from cancer throughout the world, and tens of millions of people are diagnosed with cancer worldwide. When surgery is not an option, treatment options commonly rely on the host immune system for optimal treatment efficacy. The expression of Interferon gamma (IFN-γ) by host immune cells is often associated with favorable antitumor immune responses. How IFN-γ is optimally induced in immune cells, and how IFN-γ promotes the transcription of genes downstream of the Interferon gamma receptor (IFNGR) is incompletely understood. Understanding how to induce immune cells to express optimal IFN-γ …

Investigating Evolution Through The Lens Of Ai-Driven Protein Exploration And Phylogenetic Modeling, Mataya Duncan

Graduate Theses and Dissertations

Recent advances in computational biology, artificial intelligence, and sequencing technologies have enabled new perspectives for addressing longstanding questions in evolutionary biology. Increased computational power has made large-scale simulations feasible for investigating diverse aspects of evolutionary and phylogenetic modeling. Similarly, deep learning algorithms now allow for the prediction of reasonably accurate tertiary protein structures, unlocking the potential for investigating diversity and evolution of protein structure across a broad range of organisms. This thesis presents two studies that target different aspects of evolutionary inference from unique perspectives. Yet, the share an overarching goal of presenting new perspectives on the evolutionary basis of …

Utility Of John Deere's See & Spray Ultimate In Southern U.S. Row Crops, Tristen Avent

Graduate Theses and Dissertations

Row crop producers across the southern United States are observing a boom in technological advancement to improve operational efficiency. Additionally, environmental concerns and reduced profit margins drive the adoption of targeted pesticide application technologies like the John Deere See & Spray. This technology is relatively new, introduced in 2022, and producers need more insight into the shortcomings, potential herbicide savings, and the economic benefits of adoption. Since 2021, research has been conducted in Keiser, AR, with additional collaborative projects in Mississippi, North Carolina, Indiana, and Illinois. Five different objectives were addressed in this research: 1) to determine the impact of …

Fractured Inheritance: Quantifying The Economic Impact Of Heir’S Property On Arkansas Farmers, Makenzie Mcjunkins

Graduate Theses and Dissertations

Heir’s property arises when the original owner still holds the title despite their passing, and ownership is distributed among their heirs. This was driven by barriers to retaining land ownership and access to legal support in predominantly African American populations in the early 20th century. This approach allowed land to remain in the original owner's name while granting continued ownership to future generations without formal paperwork. Additionally, allowing limited land access, land management, and disparities in capital access compared to property with direct ownership rights. This analysis examines how modern heir’s property impacts agricultural returns. This study develops a conceptual …

Structure Disorder And Magnetic Behavior Of An Olivine-Type Cathode Material, Hamida Hassan, Madalynn Marshall

Symposium of Student Scholars

Our research investigates the structure disorder and magnetic behavior of Li(Mn,Fe)PO₄, an olivine-type material relevant for lithium-ion battery applications. Using single-crystal neutron diffraction at Oak Ridge National Laboratory, we precisely determined Mn and Fe occupancy, revealing a 56% Fe and 44% Mn distribution at the atomic 4c site within the Pnma space group. Additionally, we observed significant lithium site vacancies (78% occupied), influencing the material's electrochemical and magnetic properties. The varying Mn/Fe ratio affected spin reorientation transitions, shifting from antiferromagnetic alignment along the a-axis to the b-axis. Our findings provide crucial insights for optimizing olivine-based cathodes, enhancing energy …

Assessing The Osmoregulatory Capabilities Of American Alligator Lingual Glands, Benjamin Angalet, Nicholas S. Green

Symposium of Student Scholars

Crocodilians inhabit a wide variety of aquatic, marine, and estuarine habitats on 4 continents. Saltwater-adapted species and populations face considerable osmoregulatory challenges in maintaining homeostasis with respect to plasma salt [NaCl] concentration. In addition to the kidneys and cloaca found in all crocodilians, crocodiles possess a salt-secreting lingual gland whose presence and function is less understood in other crocodilians. This study investigates the lingual gland anatomy, physiology, and potential function of American alligator (Alligator mississippiensis) populations exposed to varying salinity levels. At each of four sites (Jekyll Island, Sapelo Island, Okefenokee NWR, and Banks Lake NWR), we will capture 12 …

Can A Ceh-27/Nkx2.1 Transgene Rescue Ceh-27 Neurodevelopmental Defects?, Meryl Gilmore, Martin L. Hudson

Symposium of Student Scholars

When the development of an organism’s nervous system is interrupted or altered, it can significantly impact that organism’s ability to survive and function properly. In our lab, we use a species of nematode called Caenorhabditis elegans as a model organism to characterize the roles of important transcription factors in human neurodevelopmental disorders such as attention deficit hyperactivity disorder (ADHD). Utilizing C. elegans makes analyzing this process in a lab very easy for many reasons; they are very small, have a rapid life cycle, invariant cell lineage, transparent bodies, and most importantly, around 80% of their genes have human orthologs. In …

Sustainable Smart Farming Device With Leafit Adaptive Growth Technology, Saville Atkins, Anthony Iwejuo, Julian Pitts, Luis Mercado, Rachnicha Rojjhanarittikorn, Sandip Das, Hai Ho

Symposium of Student Scholars

For farmers, gardeners, and horticulture enthusiasts worldwide, one immutable reality is that maintaining a consistent physical presence to care for plants is not always feasible. In addition, different plants have unique needs for watering, nutrients, and environmental conditions to thrive. Failing to meet these specific needs can result in poor plant health, reduced yields, and inefficient resource usage. In this research project, we have designed and developed ‘LeaFit’ – a cutting-edge Internet of Things (IoT) device that offers a sophisticated and sustainable smart farming and gardening solution. Equipped with intelligent soil moisture, ambient temperature, humidity, and light sensors, LeaFit autonomously …

Predicting Healthcare Service Quality Based On A Kalman-Optimized Bi-Lstm-Inspired Deep Learning Model, Mohammed K. Al-Khafaji, Eman S. Al-Shamery

Karbala International Journal of Modern Science

Health is one of the most important aspects of human well-being, and access to high-quality healthcare is essential for a good quality of life. Providing top-level health services at all times is crucial. However, the research in healthcare poses significant challenges due to the diversity and variations of medical practices across different hospitals. This paper aims to tackle the challenge of data missing and scattering during data collection. Then, the quality of services (QoS) offered by healthcare facilities will be analyzed and predicted from the patient's perspective. The model begins preprocessing data by data cleaning, handling missing values, and scattering …

An Analysis Of New York’S Drug Policies: Comparing The Effectiveness Of Punitive Punishment Versus Rehabilitation In Preventing Recidivism, Zed Agarwal

Student Publications & Research

The persistent failure of punitive drug policies, exemplified by the Rockefeller Drug Laws, necessitates a critical re-examination of how society addresses drug offenses. For decades, New York State has been at the forefront of both the implementation of harsh drug sentencing laws and the subsequent development of innovative rehabilitation alternatives. The trajectory of this policy evolution reveals a profound shift in understanding the nature of addiction and the most effective approaches to reducing drug-related crime. When New York enacted the Rockefeller Drug Laws in 1973, they represented the epitome of punitive drug policy by imposing mandatory minimum sentences of fifteen …