Life Sciences Commons^™

Family Planning Information, Education And Communication With Contaceptive Use, Endah Winarni, Muhammad Dawam

Kesmas

Komunikasi Informasi dan Edukasi (KIE) berperan penting dalam meningkatkan pencapaian prevalensi kesertaan Keluarga Berencana (KB). Namun, prevalensi KB di Indonesia selama satu dekade terakhir (2002-2012) tidak meningkat secara signifikan, walaupun pelbagai kegiatan KIE KB telah dilaksanakan. Penelitian ini bertujuan untuk mengetahui faktor sosio ekonomi demografi yang berkaitan dengan KIE KB, pelbagai unsur KIE yang berkaitan dengan pemakaian KB, dan unsur KIE yang berpengaruh terhadap pemakaian KB. Analisis menggunakan sumber data Survei Demografi dan Kesehatan Indonesia 2012 dengan unit analisis perempuan berstatus kawin usia 15-49 tahun. Jumlah sampel yang dianalisis 33.465 perempuan berstatus kawin. Analisis menggunakan odds ratio atau rasio kecenderungan …

Sexual Dimorphism In Rat Vascular Function Of Type I And Type Ii Diabetes: Role Of Edrfs, Roshanak Rahimian

Science Seminar Series

Dr. Roshanak Rahimian, professor of physiology and pharmacology in the Thomas J. Long School of Pharmacy and Health Sciences will tell us about her work on understanding the mechanisms underlying the interaction of sex and diabetes in vasculature.

Over the past decade, diabetes and obesity have reached epidemic levels in developed countries, and has become one of the most serious and challenging health problems in the 21st century. Several studies suggest that diabetes affects male and female vascular beds differently. However, the mechanisms underlying the interaction of sex and diabetes in vasculature remain to be investigated. We have shown that …

The Charm Of Secrecy: Secrecy And Society As Secrecy Studies, Susan Maret

Secrecy and Society

No abstract provided.

Conformationally Superarmed S-Ethyl Glycosyl Donors As Effective Building Blocks For Chemoselective Oligosaccharide Synthesis In One Pot, Mithila Bandara, Jagodige Yasomanee, Nigam Rath, Christian Pedersen, Mikael Bols, Alexei Demchenko

Chemistry & Biochemistry Faculty Works

A new series of superarmed glycosyl donors has been investigated. It was demonstrated that the S-ethyl leaving group allows for high reactivity, which is much higher than that of equally equipped S-phenyl glycosyl donors that were previously investigated by our groups. The superarmed S-ethyl glycosyl donors equipped with a 2-O-benzoyl group gave complete β-stereoselectivity. Utility of the new glycosyl donors has been demonstrated in a one-pot one-addition oligosaccharide synthesis with all of the reaction components present from the beginning.

Structural Basis For The Recognition Of Spliceosomal Smn/B/B' Proteins By The Rbm5 Ocre Domain In Splicing Regulation, André Mourão, Sophie Bonnal, Komal Soni, Lisa Warner, Rémy Bordonné, Juan Valcárcel, Michael Sattler

Biomedical Research Institute Publications and Presentations

The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U6.U5 tri-snRNP. We show that the RBM5 OCRE domain adopts a unique β–sheet fold. NMR and biochemical experiments demonstrate that the OCRE domain directly binds to the proline-rich C-terminal tail of the essential snRNP core proteins SmN/B/B’. The NMR structure of an OCRE-SmN peptide complex reveals a specific recognition of poly-proline helical motifs in SmN/B/B’. …

The Role Of Interdisciplinary Scholarship And Research To Meet The Challenges Facing Agriculture In The 21st Century, Joshua Jay Miller

Doctor of Plant Health Program: Dissertations and Student Research

Throughout human history, scientific advancements have increased our understanding of the physical world. However, as our breadth of knowledge has increased, scholarship and research have become increasingly more specialized in order to add to the body of knowledge. University structures encourage this specialization through disciplinary learning and discovery. Although this model is necessary to continue growing the body of knowledge, the complex issues facing humanity, especially in regards to agriculture, require solutions that no single discipline can provide. These issues require an interdisciplinary approach to integrate the insights across multiple disciplines. Interdisciplinarity can be achieved through collaborative processes, but these …

Animating And Exploring Phylogenies With Fibre Plots, William D. Pearse

Biology Faculty Publications

Despite the progress that has been made in many other aspects of data visualisation, phylogenies are still represented in much the same way as they first were by Darwin. In this brief essay, I give a short review of what I consider to be some recent major advances, and outline a new kind of phylogenetic visualisation. This new graphic, the fibre plot, uses the metaphor of sections through a tree to describe change in a phylogeny. I suggest it is a useful tool in gaining an rapid overview of the timing and scale of diversification in large phylogenies.

Regulation Of Gonad Morphogenesis In Drosophila Melanogaster By Btb Family Transcription Factors, Diane Silva, Kenneth W. Olsen, Magdalena Bednarz, Andrew Droste, Christopher P. Lenkeit, Jennifer Mierisch, Edwin Chaharbakhshi, Emily R. Temple-Wood

Biology: Faculty Publications and Other Works

During embryogenesis, primordial germ cells (PGCs) and somatic gonadal precursor cells (SGPs) migrate and coalesce to form the early gonad. A failure of the PGCs and SGPs to form a gonad with the proper architecture not only affects germ cell development, but can also lead to infertility. Therefore, it is critical to identify the molecular mechanisms that function within both the PGCs and SGPs to promote gonad morphogenesis. We have characterized the phenotypes of two genes, longitudinals lacking (lola) and ribbon (rib), that are required for the coalescence and compaction of the embryonic gonad in Drosophila melanogaster. rib …

Two New Species Of Euscorpius Thorell, 1876 From Southern Turkey (Scorpiones: Euscorpiidae), Gioele Tropea, Ersen Aydın Yağmur

Euscorpius

Two new species of scorpion of the genus Euscorpius Thorell, 1876 are described from southern Turkey based on morphological ratio and trichobothrial data. E. hakani sp. n. from Mt. Eşeler, in Denizli Province in southwestern Turkey and E. aladaglarensis sp. n. from the Aladağlar Mts., in Niğde, Kayseri, and Adana Provinces. With the description of two new taxa, 17 species of Euscorpius are currently recognized in Turkey.

Insect Consciousness: Commitments, Conflicts And Consequences, Colin Klein, Andrew B. Barron

Animal Sentience

Our target article, “Insects have the capacity for subjective experience,” has provoked a diverse range of commentaries. In this response we have collated what we see as the major themes of the discussion. It is clear that we differ from some commentators in our commitments to what subjective experience is and what the midbrain is capable of. Here we clarify where we stand on those points and how our view differs from some other influential perspectives. The commentaries have highlighted the most lively areas of disagreement. We revisit here the debates surrounding whether the cortex is essential for any form …

Do We Need Demographic Data To Forecast Plant Population Dynamics?, Andrew T. Tredennick

Wildland Resources Faculty Publications

1. Rapid environmental change has generated growing interest in forecasts of future population trajectories. Traditional population models built with detailed demographic observations from one study site can address the impacts of environmental change at particular locations, but are difficult to scale up to the landscape and regional scales relevant to management decisions. An alternative is to build models using population-level data that are much easier to collect over broad spatial scales than individual-level data. However, it is unknown whether models built using population-level data adequately capture the effects of density-dependence and environmental forcing that are necessary to generate skillful forecasts.
2. Here, …

Global Ocean Data Science Ocg 250, Joanna Burkhardt

Library Impact Statements

No abstract provided.

The Inhibitory Effect On Tyrosinase From P-Coumaric Acid, Ferulic Acid And Feruloylated Oligosaccharides, Yi Jingjing, Ou Shiyi, Dong Yingyan, Chen Qiuming

Food and Machinery

This study investigated the inhibitory effects of p-coumaric acid, ferulic acid and feruloylated oligosaccharides on catalytic activities of tyrosinase by analysising tyrosinase steady state activity and enzyme kinetics. The results showed that the three compounds were found to efficiently inhibit tyrosinase monophenolase activities. The p-Coumaric acid was the strongest inhibitor followed by feruloylated oligosaccharides and ferulic acid, with the IC₅₀ value of 0.75, 3.20, 9.30 mmol/L respectively. Moreover, p-coumaric acid and ferulic acid were found to inhibit the activities of diphenolase with IC₅₀ value of 4.3, 12.7 mmol/L respectively; however, feruloylated oligosaccharides showed no inhibitory effect on diphenolase …

Effect Of Gastrodia Elata Bl. Extracts On The Biosynthesis Of Extracellular Proteins In The Grifola Frondosa Under Submerged Fermentation, Huang Zhong, Wu Tianxiang, Yang Zutao, Zhu Sijie

Food and Machinery

Gastrodia elata Bl. extracts was added into the system of Grifola frondosa submerged fermentation, and then supplement-ation of Gastrodia elata Bl. extracts were optimized according to the biomass and the synthetic quality of extracellular proteins in G. frondosa. The changes of components in G. elata Bl. extracts before and after the sterilization, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxybenzaldehyde, and parishin, were analyzed using HPLC. Moreover, the relationship of this changes between G. elata Bl. extracts and the in G. frondosa fermentation system were also investigated. The results revealed that 7% (v/v) G. elata Bl. extracts could facilitate G. frondosa to grow …

Determination Of Vanillin And Ethyl-Vanillin In Milk Powder By Gas Chromatography-Mass Spectrometry, Zheng Xuan, Huang Jin, Lin Jie, Li Ju, Wei Xiaoqun, Cai Chun, Chen Yanfen, Feng Jieli, Guo Qingyuan

Food and Machinery

A method for determination of vanillin and ethyl-vanillin in milk powder was established by auto headspace solid-phase micro-extraction coupled with gas chromatography-mass spectrometry (GC—MS), and the influence factor were also studied, such as solid phase micro-extraction head, time and temperature of adsorption and desorption. As a result, the limits of determination (LOD) of vanillin and ethyl-vanillin were 0.5 mg/kg. The spiked recovery was between 90.0% and 100%, and the RSD ranged from 1.9%～4.1%(n＝6). This method could eliminate the interference of complicated matrix, conveniently, quickly and exactly, which was applicable for quick determination of vanillin and ethyl-vanillin content in milk powder.

Determination Of Five Kinds Of Clove Phenol Anesthetic In Aquatic Product By Hplc With Molecularity Imprinted Solid Phase Extraction, He Hongjian, Huang He, Gao Ping, Liu Ya, Jiang Zhihong, Cao Zhanhui

Food and Machinery

A new method of molecularly imprinted solid phase extraction (MISPE) combined with high performance liquid chromatography (HPLC) was developed for detecting five kinds of clove phenol anesthetic in aquatic product. Using eugenol as template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross linking agent, molecularly imprinted polymers were synthesized by bulk polymerization and made into extraction column. Through the response surface analysis template molecule and functional monomer, crosslinking agent the best proportion was 1∶4. 55∶23. 21, and the recovery rate of these compounds reached 90% by using methanol as solvent and acetic acid solution as eluent. The …

On-Line Determination Of Emergency Pollution Of 3-Nitropropionic Acid In Drinking Water By Flow Injection Chemiluminescence, Zhang Xuhui, Tang Shuze, Deng Xiaoling, Zhu Yao, Wu Shizheng

Food and Machinery

On the basis of inhibition of chemiluminescence reaction of luminol and potassium permanganate in sodium hydroxide medium by 3-nitropropionic acid, a novel flow-injection chemiluminescence method for determination of 3-nitropropionic acid in drinking water was developed. A linear range for 3-nitropropionic acid determination was 1～150 mg/L. The detection limit of 3-nitropropionic acid was 0.28 mg/L and relative standard deviation (RSD) was 1.9%, with the recovery of 76.5%～93.8%. This online determination system showed a good accuracy and selectivity and has great potential application in rapid detection and alarm of emergency pollution of 3-nitropropionic acid in drinking water.

Tank Design And Parameter Optimization Of The Fruit Grain Liquid Storage, Yin Shuizhong, Huang Xiaohua, Yang Dizhou, Ding Zhihui, Zhu Can

Food and Machinery

A fruit grain beverage liquid mixing tank was designed in this study, including the structure of the cylinder block and the mixing paddle, and related parameters were provided. The numerical simulation was carried out by orthogonal test and FLUENT,to determine the influence degree of the factors, such as the installation height of the agitator, the diameter of the impeller and the rotation speed of the propeller, and the parameters of the stirred tank, were optimized to obtain the optimal parameter solution. Results: The main order of the factors that influenced the stability of the agitator concentration was as follows: the …

Design Of Extrusion-Grind Rubber Fruit Test Sheller, Fan Haiyan, Wang Tao, Wu Di, He Xiaofan

Food and Machinery

A test sheller for rubber fruit was designed and manufactured according to the principle of extrusion-grind, and it consisted of units of driving and extrusion-grind, the lectrical elements for realizing automatic clockwise and anti-clockwise running of motors, and the frame, etc. Adopting the principle of reciprocating friction and extrusion, the fruit shells were separated under the dual of friction and extrusion pressure. Our results showed that the device could perform well and was, easy to operate, especially, the hulling rate could reach 78.3% by using it. Thus this sheller could be utilized in researches on effects and the mechanism of …

Comparative Study Of The Failure Pressure Between Sealing Liquids And Gas With Magnetic Fluid, Wang Hujun, Li Decai, Zhen Shaobo, He Xinzhi, Wang Siqi

Food and Machinery

Magnetic fluid has been widely used in sealing gas, but sealing liquids with magnetic fluid is still in the research stage. In this paper, the effect of velocity difference on failure pressure was theoretically deduced. The magnetic fluid seal structure was designed and an experiment its rig was set up, and then the seal performances of sealing gas and liquids with magnetic fluid were studied. The results showed that the failure pressures of sealing liquids and gas statically under the same sealing gap were almost the same, and the failure pressure was generally not affected by the speed of the …

The Effects Of Condensing Temperature Changes On Plate Freezer With Two-Phase Ejector, Tian Xiaoyu, Wan Jinqing, Cao Xiaochen, Li Jianguo

Food and Machinery

The working performance of plate freezer with two-phase ejector in different condensing temperature was investigated in this study. Moreover, the effects of condensing temperature on freezing rate and total energy consumption of plate freezer was also studied. Then the entrainment coefficient in stable conditions and explores the effects on Jet pumps were analyzed by changing the condensing temperature. The results showed that when the condensing temperature was 25 ℃, the ejecting coefficient was the highest about 1.58. Compared with 40 ℃and 30 ℃, the energy consumption was lowered by about 3.3% and 10% at 25 ℃, and the freezing time …

Electromagnetic Vibration Quantitative Feeding System Based On Fuzzy Pid, Qi Jun, Gong Dawei, Li Yan, Yan Qingqun

Food and Machinery

To solve the problem of traditional electromagnetic quantitative feeding system in the process of the open loop control the feeding speed setting arbitrary large, not according to the intelligent control strategy for dynamic adjust the feeding speed, a quantitative electromagnetic vibration feeder was designed based on fuzzy PID control system. Taking the mechanical structure design of the system as a whole, the conduction angle of silicon controlled rectifier was obtained by theoretical analysis and mathematical relationship between amplitude, and according to this, the simplified mathematical model of electromagnetic vibration feeder second order was established. The simulation results show that the …

Linking Transcriptional And Physiological Responses To High Light Stress In A Wild-Type And A Photomorphogenic Tomato Mutant, Jordyn Radke

Theses and Dissertations

Tomato (Solanum lycopersicon) is the leading vegetable crop worldwide and an important part of a healthy human diet. In this study we used Next-Generation Sequencing, metabolomics and gene ontologies to study the effects of high light stress on the photomorphogenic hp-2dg tomato mutant. Wild-type (cv. ‘Manapal’) and mutant plants were grown for six weeks under low light and transferred to high light for one week. Illumina-based RNA Sequencing was performed and analysis of differential gene expression identified over 650 transcripts that were strongly regulated by light intensity (FC>4, p2,p<0,01). HPLC analysis of tomato leaves showed differential accumulation of phenylpropanoid metabolites such as chlorogenic acid, rutin, and caffeoylglucaric acid which was strongly dependent on genotype and light condition. Our findings show insightful differences between the wildtype and the photomorphogenic mutant as well as novel genes involved in high light adaptation in plants.

Optimization Of A Microplate Bioassay Method Of Filtering Alpha-Glucosidase Inhibitors, Wang Hongyue, Chen Zhong, Jian Xufeng

Food and Machinery

The half inhibitory concentration (IC₅₀) of alpha glucosidase inhibitors was detected based on a microplate assay method in this study in order to find the effective inhibitors for preparing hypoglycemic functional food. The reaction conditions were optimized by the four aspects, i.e., the enzyme concentration, the detection wavelength, the substrate concentration and the reaction time. The results showed that the optimum enzyme concentration ranged from 2.0 mg/mL to 3.0 mg/mL, and waves 520 nm and 492 nm were optimum among the five waves, 405, 450, 492, 520, 630 nm. Moreover, the suitable substrate concentration of sucrose ranged from …

Identification Of 81 Pseudomonas Aeruginosa By Phylogenetic Analysis Of 16s Rrna Gene Sequence, Zeng Xiaocong, Zhou Lu, Su Miaozhen, Han Zhijie, Chen Danxia

Food and Machinery

81 Pseudomonas aeruginosa from Guangdong Provincial Institute of Food Inspection were identified by 16S rRNA sequence analysis. The DNA was isolated and the sequences of 16S rRNA gene were amplified by PCR with the bacterium universal primers, and then the PCR products were sequenced after 2% agarose gel electrophoresis. Moreover, the corrected sequences were aligned with Clustal X and the phylogenetic tree was constructed by MEGA5.1. Consequently, the identified results of the 81 strains confirmed their original identification before. On the phylogenetic tree, No. 24-3-QY strain formed a separate branch with No. 100-5-JM strain and No. 106-3-JM strain. No.2 8-1-DW …

Separation, Screening And Identification Of Special Microbial Strain For Beef Meat Tenderizer, Tan Ya, Li Zongjun, Li Ke, Wu Genliang

Food and Machinery

In order to obtain microbial strain suitable for the tenderization of beef, a total of 22 bacteria of producing proteinase were isolated from traditional lobster sauce and wine song in Guizhou. With the screening by using microscopy, analyses of enzyme activities, and TPA value of beef with in treatment of fermented broth, a bacteria defined as D7 was found the optimal strain, and its enzyme activity was 71.68 U/g. The molecular biological identification results showed that it was very similar to Bacillus amyloliquefaciens (JN700123.1), and the similarity was 99%, on the basis of molecular biology identification, using 16S rDNA sequences …

Identification And Quantitative Analysis Of Soybean Milk And Rice Milk In Raw Milk, Fang Xuanqi, Yang Daiming, Liu Lansong, Zhou Binbin, Zhang Juan, Zhang Jihong

Food and Machinery

In order to identify raw milk, nonlinear chemical fingerprint of raw milk from different areas and that mixed with different content of soybean milk or rice water was established based on the steady system of “sodium bromate + manganese sulfate + Sulfate + acetone” by using nonlinear fingerprint technique . The raw milk samples that incorporated with soybean milk or rice water could be identified, and the adulterant could be judged by the characteristic information of their nonlinear chemical fingerprint. According to the relationship of parameter information and adulterant content, the relative global content of soybean milk or rice water …

Design Of Mixing Reaction Tank Of Suitable For High Viscosity Non-Newtonian Fluidossein, Han Dong, Jia Wei, Zhang Chunhui

Food and Machinery

In order to solve the problems of the material of mixed unevenness, low dissolved efficiency, uncontrollable reaction temperature and aroma loss in the conventional mixing reaction tank, a kind of mixing reaction equipment was designed and developed for high viscosity non-Newtonian fluidossein. The mixing reaction equipment consisted of tanks, feeding apparatus, and devices of vertical composite stirring, bottom emulsification stirring and the reaction volatiles collected reflux. This equipment was well suited for high viscosity non-Newtonian fluid to blend, mix and react, and able to achieve multifunctional operation with energy saving. Moreover, it helped to increase the degree of controllability of …

Modeling And Dynamic Simulation Ofautomatic Apple Oriented Mechanism, Ma Yao, Yu Jinghu

Food and Machinery

It’s difficult to distinguish between apple damage and fruit axis and calyx in image processing. This results in poor efficiency and accuracy. An apple orientation mechanism is designed, and realized orientation of apple before image acquisition, thereby reducing image processing time. It mainly includes fruit pan, synchronous wheel, fixed caster and drive shaft. ADAMS was used to simulate the orientation process of apple, which verified the feasibility of the mechanism, and sure that the apple will not scratched in the orientation process. In order to improve the efficiency of apple sorting system, the optimal scheme was obtained by simulating the …

Tea Frying Temperature Control System Based On Fuzzy Expert Control, Pan Yucheng, Lin Gaofei, Chen Xiaoli, Huang Xianzhou

Food and Machinery

Tea frying temperature has a great influence on the quality of tea.To cope with the disadvantages of the existing tea-leaf parcher temperature control system with temperature control of low precision and the longtime adjusting, the tea-leaf parcher with intelligent temperature control system we designed， which is suitable for frying high-end quality famous tea. System used AT89S52 single chip as the core, the platinum thermal thermistor as the temperature sensor and linearized the output signal of unbalanced bridge by means of current transmitter XTR106 and designed a kind of fuzzy expert controller combine with the fuzzy control theory and expert system. …