Life Sciences Commons^™

The Role Of Nutrition Education And Active Choice To Increase Fruit And Vegetable Consumption Among Second Grade Students During Lunchtime, Robyn M. Cafiero

Theses, Dissertations and Culminating Projects

Fruit and vegetable intake within the United States is considerably lower than the standards set by the United States Department of Agriculture. With this information, there is no surprise that these rates are especially low for school aged children. Although many studies have tried to incorporate programs that would effectively increase the consumption of fruits and vegetables among youngsters, many have fallen short with being able to sustain consumption over time. This study provided a way to increase fruit and vegetable consumption during lunchtime for second grade students by using a dual module of the Social Cognitive Theory and active …

Interactions In The Cpsrp Dependent Targeting Of Light Harvesting Chlorophyll Binding Protein To The Thylakoid Membrane, Rory Henderson

Graduate Theses and Dissertations

Targeting of proteins is a critical component of cellular function. A universally conserved targeting system of the cytosol utilizes a signal recognition particle (SRP) to target many proteins contranslationally to the endoplasmic reticulum in eukaryotes or the inner membrane in prokaryotes. A homologous SRP system exists in the chloroplast that delivers light harvesting chlorophyll binding proteins (LHCP) to they thylakoid membrane. The chloroplast SRP (cpSRP) is a heterodimer composed of a novel 43 kDa subunit and a 54 kDa subunit homologous to a component of the SRP system, SRP54. Many details regarding the interactions between the proteins of the cpSRP …

Membrane Chromatography For Bioseparations: Ligand Design And Optimization, Zizhao Liu

Graduate Theses and Dissertations

Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) …

Antimicrobial Inhibitory Activities Of Phenolic Extracts From Four Selected Soybean Hulls In Culture And Chicken Skin Model Systems, And Preliminary Ace-Inhibitory Activity, Rajaa Abdulkair Abutheraa

Graduate Theses and Dissertations

Soybean components provide health benefits to humans. Soybean hulls, a major by-product of the soybean processing industry consist of complex carbohydrates, proteins, lipids and polyphenols such as anthocyanidins, proanthocyanidins and isoflavones. The polyphenolic compounds in the hulls give them various colors such as black, brown, green, yellow or even a mottled appearance. Studies have reported different soybean varieties with varying total phenolic compounds in their seed hulls, which have antioxidant property. Phenolic extracts can be used as substitutes for synthetic antimicrobials and preservatives to assist in preventing the growth of pathogens such as Salmonella Typhimurium, E coli 0157:H7, and Campylobacter …

Selection Of Drought-Tolerant Soybean Lines Using A Field Screening Method And Identification Of Qtls For Slow Wilting And Nitrogen Fixation Associated With Drought-Tolerance, Mirta Beatriz Dalzotto

Graduate Theses and Dissertations

Drought causes significant yield reductions in soybean. The development of drought-tolerant cultivars is an effective alternative to overcome this abiotic stress. Slow wilting, prolonged nitrogen fixation, and minimal yield reduction under water stress play an important role in evaluating breeding materials. Despite advances in knowledge about plant responses to drought conditions, there is little information on effective methodologies for phenotype screening in the field, and some QTLs have been identified for drought tolerance. Field screenings under drought and irrigated conditions are necessary to detect drought tolerant lines. In addition, QTLs and molecular makers associated with drought tolerance traits will greatly …

Survey Of Nasal Mites (Acari: Rhinonyssidae, Ereynetidae, Turbinoptidae) In Birds From Three States In The United States, Alexis Danel Hilario Perez

Graduate Theses and Dissertations

Avian nasal mites are obligate endoparasites that spend their entire life in the respiratory system of birds. In North America, bird nasal mites are represented by different unrelated families in three different orders of mites: Rhinonyssidae (Mesostigmata), Ereynetidae (Prostigmata), and the Cytoditidae and Turbinoptidae (Astigmata). The most-diverse and most-abundant family of nasal mites is the Rhinonyssidae, in which mite species may have different levels of host specificity from host orders to families or even species level. Nasal mites in North America have been surveyed in different locations, such as studies ranging from the Gulf Coast of the US to Canada. …

Soil And Plant Property Differences Among High-Yield Soybean Areas In Arkansas, Taylor Cass Adams

Graduate Theses and Dissertations

Continued achievement of soybean [Glycine max (L.) Merr.] yields greater than 6719 kg ha-1 (100 bu ac-1) will depend on decreasing the yield gap, which is contingent on gathering more information regarding the soil physical, chemical, and microbiological environment and the main plant factors contributing to high-yield soybean. Therefore, understanding the main factor differences between high- and average-yield areas may provide insight for making management decisions to increase yields. The objectives of this study were i) to evaluate the effects of region and soil depth on soil property differences between high- and average-soybean-yielding areas, ii) to determine which soil properties …

Migration Ecology Of American Woodcock (Scolopax Minor), Joseph Daniel Moore

Graduate Theses and Dissertations

Improving the understanding of American woodcock (Scolopax minor) migration ecology has been identified as a priority information need for woodcock management. Developments in remote tracking technology and analytical techniques present an opportunity to gain insight into woodcock migratory connectivity and migration phenology and to evaluate the degree in which the current two-region (Eastern and Central) basis for woodcock management represents migratory movements. To analyze woodcock migration using band return records, I excluded observations that took place during the migratory period. Using this dataset, 17.9% of records showed crossover between management regions, higher than the < 5% crossover found in studies including non-migratory band returns. During autumn migration, woodcock from the Central Region largely migrated to destinations within the Central Region, whereas woodcock from the Eastern Region migrated to destinations across their wintering range, mixing with individuals from the Central Region. Between 2013 and 2016, I deployed 75 satellite transmitters on woodcock. I tracked the migration paths of 61 woodcock and documented 88 woodcock migrations. Average migration duration was longer during spring migration (53 days) than during autumn migration (31 days) because woodcock made a higher number of close-together migratory stopovers, not because woodcock stayed at individual stopovers longer during spring migration. Woodcock captured in the Central Management Region used 2 primary migrations routes: a Western Route and a Central Route. The Western Route ran north-south, connecting the breeding and wintering grounds of the Central Management Region. The hourglass-shaped Central Route connected an area on the wintering grounds reaching from Texas to Florida, to sites throughout northeastern North America. Woodcock following the Central Route funneled between the Appalachian Mountains and the Mississippi Alluvial Valley in western Tennessee during both autumn and spring migration. A higher than anticipated percentage (36%, n = 12) of marked woodcock captured in Texas and Louisiana and monitored during spring migration migrated to breeding-period sites in the Eastern Management Region, raising questions about the biological basis of managing woodcock as separate populations. The supplementary material includes woodcock capture information (Appendix I), information on individual stopovers (Appendix II), and migration maps for individual woodcock (Appendix III).

The Effects Of Using Cinnamon Leaf And Bark Essential Oils On Listeria Monocytogenes (L.M.), Salmonella Typhimurium (S.T.), In Model System, Strawberry Shake And Fresh Celery, And Sensory & Shelf Life Studies, Wafaa Brnawi

Graduate Theses and Dissertations

Essential oils derived from the bark and leaves of the cinnamon plant have long been used as natural preservatives and flavoring agents in different types of foods. In this study, we evaluated the antimicrobial effects of cinnamon essential oils (CEOs), obtained from cinnamon leaf or bark, against two foodborne pathogens i.e., Salmonella Typhimurium (S.T.) and Listeria monocytogenes (L.m.). Two different concentrations of microbial loading were used i.e.,109 and 104, cultured in nutrient media broth, strawberry shakes, and on celery sticks. Both CEOs of leaf and bark at 0.5% and 1% were found to completely inhibit S.T. and L.m., immediately after …

Query Theory Applications: Choice Experiments Under Oath, Attendance To Attributes, And Genetically Modified Food Labeling Policy, Nathan Paul Kemper

Graduate Theses and Dissertations

In recent years, there has been an intensifying campaign by some stakeholders regarding concern over genetically modified (GM) foods in the U.S. As a result, the issue of labeling has entered into the federal agenda. This research uses Query Theory to provide a deeper understanding of the demand for GM foods and the preferences for GM policy. Query theory is first applied to the formation of hypothetical bias in the estimation of consumers’ willingness-to-pay. To address this, the honesty oath is used as an ex-ante technique to reduce hypothetical bias.

Paper one using Query Theory in a discrete-choice experiment (DCE) …

Immunotherapeutic Strategies To Reverse Tumor Associated Immunosuppression In Breast Cancer, Sruthi Ravindranathan

Graduate Theses and Dissertations

Heterogenous cancers such as breast carcinoma, would highly benefit from personalized vaccine approaches such as autologous tumor cell vaccines (ATCVs). By using cancer cells that are isolated from self, this approach has the potential to generate a polyclonal immune response against tumor antigens, that are specific to the individual. However, so far, ATCVs against breast cancers have not had any clinical success due to the poor immunogenicity of the malignant cells.

In this study, for the first time, we determined tumor derived G-CSF to play a major role in affecting immune response against breast cancer ATCVs. By using CRISPR/Cas9 genome …

Effects Of Harvest Conditions And Thickness Fractionation On Physicochemical Properties, Cooking And Sensory Characteristics Of Long Grain Rice, Sara Esther Jarma Arroyo

Graduate Theses and Dissertations

Previous research has demonstrated the extensive impacts of various environmental and processing conditions on rice milling and processing characteristics. Nevertheless, little is known about the influences of these conditions on cooking and sensory qualities of rice. The present study aimed to determine the impact of cultivating location, harvest moisture content (HMC), and thickness fractionation on the physicochemical properties, and cooking and sensory characteristics of long-grain rice. Four long-grain rice cultivars (purelines: Cheniere and V3501, and hybrids: XP760 and XL753) were cultivated at Harrisburg, AR and Alvin, TX, and harvested at three HMC. Rough rice lots were conditioned and a portion …

Effect Of Delayed Wrapping And Wrapping Source On Intake And Digestibility Of Alfalfa Silage In Gestating Sheep, Valens Niyigena

Graduate Theses and Dissertations

Baling silage with oxygen can result in dry matter deterioration and reduce silage intake by animals. This study was conducted to investigate the effects of two different wrapping sources and time intervals between baling and wrapping on intake and digestibility of alfalfa silage. The second objective was to assess the correlation of alfalfa silage fermentation parameters with intake and digestibility parameters in gestating sheep. Alfalfa silage was baled in large round bales then wrapped with plastic either with (KURA) or without (SUN) an oxygen-limiting barrier either the day of baling or 1, 2 or 3 d after baling. Beginning in …

Phenotypic Analysis And Localization Of Leukocytes At The Site Of Intradermal Injection Of Phytohemagglutinin In Chickens, Kallie Alyce Sullivan

Graduate Theses and Dissertations

Phytohemagglutinin (PHA) is commonly used to gain insight into an individual’s cellular immuno-competence. PHA is typically injected intradermally (i.d.) into the skin (e.g. wing web) and the tissue swelling response is then monitored, whereby the extent of the swelling positively relates to the individual’s cellular immune system capabilities. Although i.d. injected PHA was shown to stimulate mononuclear cell- and basophil-infiltration to the site of injection, reports on temporal, qualitative and quantitative aspects of the local PHA response are limited. The objective of this study was to use the growing feather (GF) as a cutaneous test-site to assess and monitor the …

Bioorthogonal Reactions: Synthesis And Evaluation Of Different Ligands In Copper Catalyzed Azide-Alkyne1,3-Dipolar Cycloaddition (Cuaac), Zainab Abdullah Almansaf

Graduate Theses and Dissertations

The Copper CatalyzedAzide-Alkyne1,3-Dipolar Cycloaddition (CuAAC) reaction has unique features that qualify it to be one of the best click reactions. Its applications have been shown in different aspects and for multiple purposes. The oxidative degradation of biological systems (labile proteins and live cells) is, however, generally recognized as the major problem when using this reaction in living systems. Reactive oxidation species can be easily produced in the presence of copper(II), ascorbate and air, and this is the main cause of toxicity. However, the uses of ligands have shown a major impact on reducing copper toxicity, protecting Cu(I) from the redox …

Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown

Graduate Theses and Dissertations

AIM: Sarocpenic obesity is a national concern within the United States because this metabolic syndrome is tied with reduced mobility and quality of life. Both obesity and aging are associated with insulin-resistance, chronic low-grade inflammation and muscle weakness. Skeletal muscle regeneration is a process that involves the coordinated effort of myogenic regulatory factors (MRFs), inflammatory signaling, and extracellular matrix (ECM) remodeling for optimal regeneration. It has been demonstrated that obesity and aging have a reduction in muscle regeneration. It has not been examined if sarcopenic obesity will further reduce muscle mass and the regenerative process. The purpose of this study …

Fishes As A Template For Reticulate Evolution: A Case Study Involving Catostomus In The Colorado River Basin Of Western North America, Max Russell Bangs

Graduate Theses and Dissertations

Hybridization is neither simplistic nor phylogenetically constrained, and post hoc introgression can have profound evolutionary effects. Most studies have focused on tractable model systems, rather than organisms with complicated phylogenetic histories. Finescale Sucker (genus Catostomus) in western North America is recognized as a paradigm of fish hybridization. Yet, its extent of historic and contemporary introgression is largely unstudied, an aspect that impedes the resolution of its phylogeny as a baseline for conservation. To explore reticulation in this group, I assayed variation of 20 Catostomus species across temporal and geographic scales by analyzing hundreds of samples and employing a combination of …

Daily Variability Of Body Weight And Hydration Markers In Free Living Men And Women, Lynndee Grace Summers

Graduate Theses and Dissertations

Body weight and hydration markers change greatly during strenuous exercise, especially in the heat. However, in a non-athletic population, changes in body weight and hydration markers may not be so obvious. It is important to classify the normal fluctuation of these measurements for future studies in order to delineate when an intervention results in a change outside of what can be expected during normal daily living. PURPOSE: The purpose of this study was to describe the normal fluctuations in body weight and urine hydration markers over the course of 29 days. METHODS: One-hundred two male and female participants, ranging from …

Cover Crop Establishment And Potential Benefits To Arkansas Farmers, Ashley Elizabeth Humphreys

Graduate Theses and Dissertations

Soybean farmers in Arkansas need best management practices (BMPs) that maximize the benefits of using cover crops including planting date and fertilization recommendations. An evaluation of cover crop species, planting dates, seeding rates, fertilizer rates, and N accumulation aids in providing these BMPs. The first objective of this research is to assess the effect of planting date on biomass production, as well as looking at the interaction of seeding rate or fertilizer rate for legumes or non-legumes, respectively, using Austrian winter pea (Pisum sativum), cereal rye (Secale cereale), black oats (Avena strigosa), wheat (Triticum aestivum), and tillage radish (Raphanus sativus). …

Yeast Dynamin Associates With The Garp Tethering Complex For Endosome-To-Golgi Traffic, Uma Saimani

Graduate Theses/Dissertations

Yeast dynamin, Vacuolar Protein Sorting 1 (Vps1), has been implicated in recycling proteins and lipids from the endosome to the trans-Golgi network (TGN). Previous research showed a genetic interaction of Vps1 with all components of the GARP tethering complex, which anchors vesicles at the late Golgi membrane. I used the yeast two-hybrid system and pinpointed a 33 amino acid segment of Vps51, a GARP subunit, that interacts with Vps1. Based on sequence homology between Vps51 and its mammalian homolog ANG2 in the 33 amino acid stretch, I identified two key residues of Vps51, E127, and Y129, that bind Vps1. The …

Modeling Reef-Coral Response To Climate Change, Christopher William Cacciapaglia

Theses and Dissertations

Coral reefs are one of the most diverse ecosystems on the planet. They provide goods and services to millions of people living in the coastal tropics. Recently, however, rising sea-surface temperatures have been threatening reef corals, causing episodes of thermal stress that lead to coral bleaching, mortality, and changes in reef composition. This increase in ocean temperature, along with the melting of glaciers and polar ice caps, is also causing an increase in sea level, which is threatening to ‘drown’ reefs that cannot keep up with sea-level rise. Some early models predicted that nearly all coral species would be unable …

Bioenergy And Carbon Farming Opportunities In The Pilbara, Robert Sudmeyer, Kim Brooksbank, David Rogers

Bulletins 4000 -

The Pilbara region covers 270 000 square kilometres of north-west Western Australia. Its main agricultural land use is pastoralism, with beef cattle grazing native pastures. Currently, only 24km² is under irrigation, with irrigated fodder the principal crop, but this could expand to 100km². This expansion has the potential to significantly broaden the economic base of the Pilbara.

Irrigation and the opportunities for changing land use and management may facilitate greater participation in the carbon economy by Pilbara land managers. Bioenergy feedstocks could be sourced from purpose-grown crops or agricultural wastes. Carbon farming activities may be facilitated by …

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 Regulates Lps-Induced Inflammation And Alveolar Remodeling In The Developing Lung., Heather Menden, Sheng Xia, Sherry M. Mabry, Angels Navarro, Michael F. Nyp, Venkatesh Sampath

Manuscripts, Articles, Book Chapters and Other Papers

In premature infants, sepsis is associated with alveolar simplification manifesting as bronchopulmonary dysplasia. The redox-dependent mechanisms underlying sepsis-induced inflammation and alveolar remodeling in the immature lung remain unclear. We developed a neonatal mouse model of sepsis-induced lung injury to investigate whether nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) regulates Toll-like receptor (TLR)-mediated inflammation and alveolar remodeling. Six-day-old NOX2

Oral Microbial Burden Of Periodontal Pathogens Among Orthodontic Patients, David Jolley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objectives

Many research studies involving orthodontic patients have a natural inclination to focus on changes in levels of cariogenic pathogens after bracket placement, and very few studies examine the role of changes of periodontal pathogens – particularly among adult patients. Interestingly, recent evidence suggests that increased levels of a specific periodontal pathogen, Fusobacterium nucleatum, may elevate risk for development of colon cancer in adults through direct pathways. Based upon this new evidence, the objective of the current study was to screen saliva samples taken from orthodontic patients to determine the prevalence of periodontal pathogens, including F. Nucleatum, T. denticola, and …

Differential Cbct Analysis Of Collum Angles In Maxillary And Mandibular Anterior Teeth In Patients With Different Malocclusions, Erin Shane Wan Ma

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: The collum angle is an angular measurement of the difference between the longitudinal axis of the crown and the longitudinal axis of the root. The aim of this study was to determine the mean collum angles for all maxillary and mandibular anterior teeth. In addition, the collum angles of different molar and skeletal classifications were compared for each anterior tooth.

Methods: Based on patient records obtained from the University of Nevada, Las Vegas Department of Orthodontics and Dentofacial Orthopedics, 326 CBCT scans were selected and divided into four molar and skeletal classifications. The patients were divided into Class I, …

Behavioral Reactions To Emotional And Physical Infidelity: An Evolutionary Perspective, Mandy Walsh Walsh

UNLV Theses, Dissertations, Professional Papers, and Capstones

This thesis examined the effects of sex and type of partner infidelity, including both physical (i.e., sexually involved with another person) and emotional (i.e., emotionally involved with another person) infidelity, on mate abandonment behaviors. Previous research has demonstrated sex differences in emotional responses to infidelity, where men react more negatively to physical infidelity and women react more negatively to emotional infidelity. While various studies have investigated perceived behavioral reactions using imagined scenarios, this study expands current research by utilizing actual retrospective reports. It was hypothesized that males would engage in significantly more mate abandonment behaviors after experiencing a physical infidelity, …

Effect Of Fragmentation And Habitat Type On Coastal Nekton In Mississippi, Thomas Bennett Sevick

Master's Theses

Coastal wetlands are extremely productive ecosystems that support an abundance of organisms at higher tropic levels. Coastal wetlands also act as important buffers from storms and help protect major cornerstones of coastal economies, such as tourism and fisheries. Despite the clear need for the protection of these habitats, anthropogenic use of coastal wetlands has increased in frequency and intensity, resulting in the fragmentation of once continuous habitats. A central challenge to assessing the impact of marsh fragmentation is the lack of quantitative distribution and abundance data from specific habitat types. This is especially true for species that are not commercially …

Effects Of An 18- Week Strength, Conditioning, And Circuit Training Program On Weight Loss In A Middle Aged Adult Male, Aryeh D. Spingarn

Publications and Research

Aryeh Spingarn Queens College, City University of New York Department of Family, Nutrition, and Exercise Sciences, Effects of an 18-week strength, conditioning, and circuit training program on weight loss in a middle-aged male. Purpose: The purpose of this study was to examine the effects of a strength, conditioning, and circuit training program on weight loss in a middle age adult. Methods: The subject participated in an 18-week strength and conditioning program 2 days per week for one hour per session, totaling 2 hours of training time per week. The first 12 weeks of the program included only strength training for …

Rapamycin Increases Length And Mechanosensory Function Of Primary Cilia In Renal Eptihelial And Vascular Endothelial Cells, Rinzhin T. Sherpa, Kimberly F. Atkinson, Viviana P. Ferreira, Surya M. Nauli

Pharmacy Faculty Articles and Research

Primary cilia arebiophysically-sensitive organelles responsible for sensing fluid-flow and transducing this stimulus into intracellular responses. Previous studies have shown that the primary cilia mediate flow-induced calcium influx, and sensitivity of cilia function to flow is correlated to cilia length. Cells with abnormal cilia length or function can lead to a host of diseases that are collectively termed as ciliopathies. Rapamycin, a potent inhibitor of mTOR (mammalian target of rapamycin), has been demonstrated to be a potential pharmacological agent against the aberrant mTOR signaling seen in ciliopathies such as polycystic kidney disease (PKD) and tuberous sclerosis complex (TSC). Here we look …

A Tale Of Two Antennules: The Performance Of Crab Odor-Capture Organs In Air And Water, Lindsay D. Waldrop, Laura A. Miller, Shilpa Khatri

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Odour capture is an important part of olfaction, where dissolved chemical cues (odours) are brought into contact with chemosensory structures. Antennule flicking by marine crabs is an example of discrete odour capture (sniffing) where an array of chemosensory hairs is waved through the water to create a flow–no flow pattern based on a narrow range of speeds, diameters of and spacings between hairs. Changing the speed of movement and spacing of hairs at this scale to manipulate flow represents a complicated fluid dynamics problem. In this study, we use numerical simulation of the advection and diffusion of a chemical gradient …