Life Sciences Commons^™

The Hemostatic Effects Of Acute Exposure To Colored Cornstarch Powder During A 5k Run, Robert C. Allsbrook

Senior Honors Projects, 2010-2019

PURPOSE: To examine the acute hemostatic effects of particulate matter (PM) in the form of colored corn starch powder during a 5 kilometer race. METHODS: 10 recreationally active adults completed two 5k runs, one with color and one without color. 10 mL blood samples were taken 6 hours prior to the trial and immediately following the trial. PAI-1 activity, FVIII antigen, and tPA activity were measured using an ELISA. RESULTS: No significant main effects or interaction effects (P<0.05) were observed among any of the variables although a trend (P = 0.082) was observed for increased PAI-1 activity during exercise in the color condition. CONCLUSIONS: There is a trend towards increased PAI-1 activity levels during exercise during color runs that could be due to increased inflammation. Furthermore, the lack of increased FVIII:ag and tPA activity suggests that the intensity of the exercise may not have been adequate and further research should be conducted in this area.

Effect Of Reproductive Efficiency In Regards To Workload On Peripheral Circulations Of Blood Cortisol And Leptin In The Open Mare, Michaelle K. Coker

Electronic Theses and Dissertations

ABSTRACT

Effect of Reproductive Efficiency in Regards to Workload on Peripheral Circulations of Blood Cortisol and Leptin in the Open Mare

(December, 2016)

Michaelle Kathleen Coker, B.S., Texas A&M University

Thesis Director: Dr. John Michael Mehaffey

Quarter and Paint horse mares (n= 9) were randomly assigned to 1 of 3 groups after being blocked based by expected conception date. Groups consist of mares on no work (NW), light work (LW), and moderate work (MW). Blood was taken weekly via jugular venipuncture prior to first heat cycle from each mare. Additionally, blood was taken at time of ovulation and then weekly …

Mitigating The Impacts Of Human Land-Use Change On Biodiversity: With A Focus On Large Migratory Herbivores, Kina Rebekah Murphy

Biology ETDs

Land-use change, commercial over-harvesting of species, and climate change are recognized as the main drivers of biodiversity loss. As a result, it is estimated that 30% of the planet’s biodiversity may go extinct by 2050. This dissertation focuses on how to mitigate the impacts of land-use change on biodiversity. I focus on large migratory herbivores because they are among the most heavily impacted by global change due to their large home range requirements. Habitat fragmentation, illegal hunting, and human-wildlife conflicts are among the biggest threats to large herbivores and result from land-use change. For this reason, my first chapter focuses …

Impacts Of Long-Term Precipitation Manipulation On Hydraulic Architecture, Xylem Function, And Canopy Status In A Piñon-Juniper Woodland, Patrick J. Hudson

Biology ETDs

The Southwestern US is predicted to become hotter and drier, as global climate change forces increasing temperatures and variability in timing and size of precipitation inputs. Drought stress has become more frequent in recent decades, and resulted in massive forest mortality in piñon-juniper woodlands. During recent severe droughts (2000-2003, 2009-2012), piñon pine (Pinus edulis Englem.) suffered disproportionately high mortality compared to co-occurring one-seed juniper (Juniperus monosperma [Engelm.] Sarg.). A large-scale precipitation manipulation experiment was established in a piñon-juniper woodland in central New Mexico to test hypotheses regarding tree survival and mortality with respect to altered water regimes. Our …

Integrating Habitat Suitability Modeling And Radio Telemetry To Describe Habitat Use Of The Western Massasaugas, Sistrurus T. Tergeminus, In Texas, Mitchell R. Barazowski

Biology Theses

Habitat suitability modeling using the software package MaxEnt (Phillips, Anderson, & Schapire, 2006) is a popular method for describing the habitat of rare species. MaxEnt uses “presence only” data to develop models; however presence data are highly skewed towards areas of high detection probability and these areas may not represent the full range of habitat use. Thusly, predictions from models developed using only data from areas with high detection probability may not represent all suitable habitat. This study tested the ability of MaxEnt models developed using three different data sets to accurately describe Western Massasauga (Sistrurus t. tergeminus) habitat at …

Changes In Mitochondrial Homeostasis And Redox Status In Astronauts Following Long Stays In Space, Hiroko P. Indo, Hideyuki J. Majima, Masahiro Terada, Shigeaki Suenaga, Kazuo Tomita, Shin Yamada, Akira Higashibata, Noriaki Ishioka, Takuro Kanekura, Ikuya Nonaka, Clare L. Hawkins, Michael J. Davies, Daret K. St Clair, Chiaki Mukai

Toxicology and Cancer Biology Faculty Publications

The effects of long-term exposure to extreme space conditions on astronauts were investigated by analyzing hair samples from ten astronauts who had spent six months on the International Space Station (ISS). Two samples were collected before, during and after their stays in the ISS; hereafter, referred to as Preflight, Inflight and Postflight, respectively. The ratios of mitochondrial (mt) to nuclear (n) DNA and mtRNA to nRNA were analyzed via quantitative PCR. The combined data of Preflight, Inflight and Postflight show a significant reduction in the mtDNA/nDNA in Inflight, and significant reductions in the mtRNA/nRNA ratios in both the Inflight and …

Why We Still Need To Worry About Bees, Meaghan Lee Callaghan

Capstones

American honey bees, and other native bee species, are still in decline, though the specter of colony collapse disorder may be fading behind us. Colony decline, the loss of bees overwinter experienced across the country at a quarter to third lost per hive (sometimes more), is now expected. Losses can include those from colony collapse disorder. The author discusses the different causes for colony decline and speaks to bee health scientists and local beekeepers. Read more at: http://www.meaghanleecallaghan.com/capstone/index.html

Inquiry-Based Biotechnology Education For Kent Intermediate School District Early College Program, Meāgan N. Treadway

Masters Theses

Growth in the field of biotechnology, combined with the ability to access information instantaneously, requires a new model of science education that will nurture deeper understanding and higher order thinking to develop a scientifically literate population. Inquirybased learning is a student-centered model built on the theoretical framework of constructivism, which allows students to learn in a way that reflects how scientists come to understand the natural world. This project aimed to address the need for an inquiry-based biotechnology curriculum in a local Early College program by developing, piloting, revising, and implementing an inquiry-based biotechnology unit while simultaneously evaluating the impact …

The Role Of Diaphanous In Ring Canal Development In Drosophila Melanogaster, Alexandra M. Kindred

Undergraduate Honors Thesis Collection

Infertility is a widespread condition that does not always have a known cause, and for which we often do not have a cure. One potential cause of infertility is defects in gametogenesis, or the formation of sperm and egg. During gametogenesis in most organisms, the developing sperm and egg are connected to each other or to supporting cells through intercellular bridges, allowing transfer of materials between cells. Defects in these connections can lead to infertility. The developing fruit fly egg is an excellent model system to study intercellular bridges, or ring canals. Rich in f-actin and actinbinding proteins, ring canals …

A Cloud-Based Workflow To Quantify Transcript-Expression Levels In Public Cancer Compendia, P J. Tatlow, Stephen R. Piccolo

Faculty Publications

Public compendia of sequencing data are now measured in petabytes. Accordingly, it is infeasible for researchers to transfer these data to local computers. Recently, the National Cancer Institute began exploring opportunities to work with molecular data in cloud-computing environments. With this approach, it becomes possible for scientists to take their tools to the data and thereby avoid large data transfers. It also becomes feasible to scale computing resources to the needs of a given analysis. We quantified transcript-expression levels for 12,307 RNA-Sequencing samples from the Cancer Cell Line Encyclopedia and The Cancer Genome Atlas. We used two cloud-based configurations and …

Defining The Role Of Phosphorylation And Dephosphorylation In The Regulation Of Gap Junction Proteins, Hanjun Li

Theses & Dissertations

Gap junctions are intercellular channels that permit the free passage of ions, small metabolites, and signaling molecules between neighboring cells. In the diseased human heart, altered ventricular gap junction organization and connexin expression (i.e., remodeling) are key contributors to rhythm disturbances and contractile dysfunction. Connexin43 (Cx43) is the dominant gap junction protein isoform in the ventricle which is under tight regulation by serine/tyrosine phosphorylation. Phosphorylation and dephosphorylation regulate many aspects of Cx43 function including trafficking, assembly and disassembly, electrical and metabolic coupling at the plaque, as well as to modulate the interaction with other proteins.

Serine phosphorylation has long been …

Chimerscope: A Novel Alignment-Free Algorithm For Fusion Gene Prediction Using Paired-End Short Reads, You Li

Theses & Dissertations

Fusion genes are those that result from the fusion of two or more genes, and they are typically generated due to the perturbations in the genome structure in cancer cells. In turn, fusion genes can contribute to tumor formation and progression by promoting the expression of an oncogene, deregulation of a tumor-suppressor, or producing much more active abnormal proteins. More importantly, oncogenic fusion genes are specifically expressed in the tumor cells, which provide enormous diagnostic and therapeutic advantages for cancer treatment. With the development of next-generation sequencing (NGS) technology, RNA-Seq becomes increasingly popular for transcriptomic study because of its high …

Dna Polymerase Zeta-Dependent Mutagenesis: Molecular Specificity, Extent Of Error-Prone Synthesis, And The Role Of Dntp Pools, Olga V. Kochenova

Theses & Dissertations

Despite multiple DNA repair pathways, DNA lesions can escape repair and compromise normal chromosomal replication, leading to genome instability. Cells utilize specialized low-fidelity Translesion Synthesis (TLS) DNA polymerases to bypass lesions and rescue arrested replication forks. TLS is a highly conserved two-step process that involves insertion of a nucleotide opposite a lesion and extension of the resulting aberrant primer terminus. The first step can be performed by both replicative and TLS DNA polymerases and, because of non-instructive DNA lesions, often results in a nucleotide misincorporation. The second step is almost exclusively catalyzed by DNA polymerase ζ …

Amino Acid Catabolism In Staphylococcus Aureus, Cortney Halsey

Theses & Dissertations

Staphylococcus aureus must rapidly adapt to a variety of carbon and nitrogen sources during invasion of a host. Within a staphylococcal abscess, preferred carbon sources such as glucose are limiting, suggesting S. aureus survives through the catabolism of secondary carbon sources. S. aureus encodes pathways to catabolize multiple amino acids including those that generate pyruvate, 2-oxoglutarate, and oxaloacetate. To assess amino acid catabolism, S. aureus JE2 and mutants were grown in complete defined medium containing 18 amino acids but lacking glucose (CDM). A mutation in glutamate dehydrogenase (gudB), which generates 2-oxoglutarate from glutamate, abrogated growth in CDM suggesting …

The Functions Of The Cid And Lrg Operons In S. Aureus Programmed Cell Death, Xinyan Zhang

Theses & Dissertations

Staphylococcus aureus cid/lrg operons regulate the formation of S.aureus biofilm formation and programmed cell death based on previous in vivo work done in Dr. Bayles's lab. cid operon, which encodes CidA/CidB/CidC proteins, has been shown to be an effector in leading to the lysis and death of the S.aureus; While lrg operon, encoding LrgA and LrgB proteins, is an inhibitor of the lysis and death. Recent studies suggest that CidA behaves like holin proteins from bacterial phage, by increasing the murein hydrolysis activity under aerobic culturing conditions. LrgA, together with LrgB, appears to inhibit this function. …

Role Of Cbl-Family Ubiquitin Ligases As Critical Negative Regulators Of T Cell Activation And Functions, Benjamin Goetz

Theses & Dissertations

Adaptive T cell immunity is essential for defense against foreign antigens and immune surveillance against cancer. Tight regulation of T cell activation is required to avoid autoimmunity to self-antigens or protracted inflammation after foreign antigens are cleared. Incomplete or inappropriate stimulation leads to an active shutdown of T cell activation called anergy. The Casitas B-lineage Lymphoma (CBL)-family of ubiquitin ligases (E3s) are essential negative regulators of T cell activation that impinge on thymic selection as well as anergy induction programs. Single gene studies show that CBL is critical during T cell development while CBL-B plays an essential role in peripheral …

Global Gene Expression Profiling Of Healthy Human Brain And Its Application In Studying Neurological Disorders, Simarjeet K. Negi

Theses & Dissertations

The human brain is the most complex structure known to mankind and one of the greatest challenges in modern biology is to understand how it is built and organized. The power of the brain arises from its variety of cells and structures, and ultimately where and when different genes are switched on and off throughout the brain tissue. In other words, brain function depends on the precise regulation of gene expression in its sub-anatomical structures. But, our understanding of the complexity and dynamics of the transcriptome of the human brain is still incomplete. To fill in the need, we designed …

Mitogen And Morphogen Signaling Dysregulation: Pathophysiological Influence In Pancreatic Cancer And Alzheimer’S Disease, Eric Cruz

Theses & Dissertations

Although the etiology of a particular disease will vary, there are genetic and epigenetic bottlenecks that frequently converge resulting in dysregulation of mitogenic and morphogenetic signaling. This propensity is acutely experienced in malignancy and neurodegenerative disease.

Here, we have first investigated the role of dysregulated signaling in the context of pancreatic cancer (PC). Morphogenetic signaling has been regarded as a pleiotropic pathway with the potential to promote and inhibit metastatic features. Our investigation of bone morphogenetic protein 2 (BMP-2), an archetypical member of the BMP superfamily, has revealed the presence of extracellular, intracellular, and long non-coding RNA products. Our findings …

Staphylococcus Aureus Biofilms Interfere With Macrophage Antimicrobial Responses Through Differential Gene Regulation, Toxin Production, And Purine Metabolism, Tyler D. Scherr

Theses & Dissertations

Staphylococcus aureus (S. aureus) is an opportunistic pathogen that is a leading cause of both nosocomial and community-associated infections. Armed with a myriad of virulence factors and the propensity to form a biofilm on native tissues and implanted medical devices alike, S. aureus infections represent a very real public health threat, the treatment of which results in an excessive economic burden. S. aureus biofilm infections are notoriously recalcitrant to antibiotic therapy and adept at evading and neutralizing the host immune antimicrobial response. Previous studies from our laboratory have shown that S. aureus biofilms are able to cause persistent …

Regulation Of Alteration/Deficiency In Activation 3 (Ada3) By Acetylation And Its Role In Cell Cycle Regulation And Oncogenesis, Shashank Srivastava

Theses & Dissertations

The ADA3 (Alteration/Deficiency in Activation 3) protein is a transcriptional adaptor protein that was initially discovered as a component of several HAT (Histone Acetyltransferase) complexes, the enzyme complex responsible for histone acetylation, which is a prerequisite for transcription. Earlier the studies from Dr. Band’s laboratory and that of others’ have deciphered a crucial role of ADA3 in cell cycle regulation (both through G₁/S and G₂/M phase transitions) and in maintaining the genomic stability.

While our laboratory investigated the mechanism behind the role of ADA3 in G₁/S transition, the same remained unknown for G_{2 …}

Motor Control In Individuals With Multiple Sclerosis, David Arpin

Theses & Dissertations

This dissertation explored motor control in individuals with multiple sclerosis (MS) by quantifying the behavioral and neurophysiological deficits present in these individuals. We behaviorally quantified the precision of the ankle plantarflexor musculature of individuals with MS. Our results indicated that the individuals with MS had a greater amount of variability in the precision of the isometric ankle torques, and that this greater variability was related to decreased walking performance. To further explore whether these motor control deficits were due to aberrant cortical activity associated with planning motor actions, we used magnetoencephalography to assess the motor planning and execution stages of …

Territoriality And Spatial Structure In The Green Anole, Anolis Carolinensis, William D. Weber Jr.

LSU New Orleans Theses and Dissertations

Anolis carolinensis has been a model organism for ecology and evolutionary biology since the seventies, yet there are still understudied aspects of their ecology. A five-year study has provided microsatellite genotypes to be used in building a pedigree and assess relatedness, enabling us to evaluate the spatial distribution of an urban population of A. carolinensis. Results indicate no correlation between a male’s size and the distance others keep from it; however, males belonging in the heavyweight morph are dictating the spatial distribution in this population. In addition, juvenile dispersal of male offspring and partial philopatry of female offspring are …

Target Validation And Pharmacological Characterization Of Novel Nmdar Modulators, Kiran Sapkota

Theses & Dissertations

The N-methyl-D aspartate receptors (NMDARs) are ligand-gated ion channels, which play important roles in learning and memory. Excessive activity of NMDARs is implicated in damage due to stroke and neurodegenerative diseases, whereas hypoactivity of NMDARs contributes to schizophrenia. The initial goal of my dissertation is to evaluate the potential role of the GluN2D-containing NMDARs in neuropathological, behavioral and cognitive alterations associated with schizophrenia and characterize the pharmacology and mechanisms of action of NMDAR modulators which could potentially be used to modulate these receptors in schizophrenia.

A subanesthetic dose of the NMDAR antagonist ketamine elicits symptoms of schizophrenia. This property led …

Variation In Female Mating Behavior And Success In The Damselfly, Calopteryx Maculata, Suzanne E. Allison

Masters Theses, 2010-2019

Traditionally, the study of sexual selection has focused on the evolution of elaborate male traits and how they enhance the ability to out-compete other males directly (access to females) and indirectly (access to desirable territories or resources). Female trait studies have focused most on evolved preferences for male traits. While we know much about how sexual selection acts on males, there is a deficit of equivalent study on females. In insects, including damselflies, male size and pigmentation are positively correlated with fat reserves and immune abilities, and therefore with male competitive ability. Here, we show that phenotypic variation that has …

Vertical Movement Of The Endangered James Spinymussel (Pleurobema Collina) And The Notched Rainbow Mussel (Villosa Constricta) In Response To Floods At Different Temperatures And Substrates: Implications For Conservation And Management, Dorottya Boisen

Masters Theses, 2010-2019

Freshwater mussels are keystone species in their ecosystems, improving water and substrate quality for other organisms while they are alive, and buffering stream pH and providing shelter with their shells after they die. Approximately 70% of all freshwater mussel species are endangered globally. The James Spinymussel (Pleurobema collina) is a critically endangered species that lives in disparate tributaries of the James River of Virginia. Like other freshwater mussels, they have a cryptic appearance and behavior, making them difficult to find and therefore, to study and conserve. Previous studies indicate a higher percentage of mussels are surfaced during their …

Occurrence, Antibiotic Resistance, And Survival Of Fecal Enterococci In Turkey Litter, Steven Glynn Mcbride Ii

Masters Theses, 2010-2019

The United States Environmental Protection Agency’s National Water Quality Inventory and the Commonwealth of Virginia’s 305(b)/303(d) Water Quality Assessment Integrated Report show fecal bacteria to be the most common cause of impairment for both streams and estuaries. Human and animal sources have both been identified as significant contributors of pathogenic bacteria to surface waters. In this study, turkey litter from a farm in Shenandoah County, VA was surveyed for total culturable bacteria and total culturable enterococci before and after a transition to organic rearing practices. The enterococci were identified to species phenotypically using the Biolog Microbial Identification System and resistance …

A Bioinformatics Methodology For The Annotation And Analysis Of Sperm Chromatin Remodeling Proteins In Sequenced Drosophila Species, Zain A. Alvi

Seton Hall University Dissertations and Theses (ETDs)

Spermatogenesis is the process by which mature functional spermatozoa are formed, and is initiated in the stem cell niche of the testes. During the post-meiotic stage of spermatogenesis, spermiogenesis, transition proteins facilitate the transformation of chromatin from a histone-based nucleosome structure to a protamine-based nucleosome structure. This study is aimed at analyzing genomic, transcript, and protein sequences of transition proteins in 13 sequenced Drosophila species. The Drosophila melanogaster spermatid specific transition protein-like protein (Tpl^94D) was used as the reference sequence in this study. An extensive bioinformatics approach was employed in establishing the Tpl^94D orthologs. We identified …

Flight Of The Freshwater Fish, Michael H. Wilson

Capstones

Michael H. Wilson

Capstone Abstract

December 27, 2016

Flight of the Freshwater Fish

The Hudson River provides for millions of people as a path for commercial and private transportation, a source of food and energy, and perhaps most importantly for many living in the tri-state area as a destination for recreation and relaxation. The most overlooked feature of the river is how the wildlife shows clear signs of a changing climate and rapid environmental response to the impacts of global warming on the river.

Entire populations of fish species in the lower Hudson have been forced to leave the river …

Phylogenetics, Biogeography, And Climate Niche Variation Of South Pacific And Hawaiian Psychotria, Elaine Zhang

Master's Theses

Why do some species have broad geographic distributions, while other species are confined to a narrow distribution? Species age, ecological niche, or dispersal traits may help explain why some insular species are abundant and found on many islands, while others are rare and restricted to one island. In this study, I inferred a robust, time-calibrated phylogeny of the Hawaiian Psychotria, using two nuclear and eight chloroplast loci, sampling 67 individuals. I coupled my phylogenetic hypothesis with climatic data, ecological niche modeling, and morphological dispersal characteristics to explain the variation in number of islands occupied by each species. My inferred phylogeny …

American Milk: The Raw Deal, Dan Heching

Capstones

This report takes a look at the difficulty in procuring raw milk, an increasingly desired alternative to processed dairy, in New York City. It also tells the story of one woman's journey to tasting raw milk for the first time, despite reservations.

link: https://social.shorthand.com/MoodyHeching/uyYgLmuoNp6/american-milk-the-raw-deal