Life Sciences Commons^™

Fish Oil With Higher Dha Content And Voluntary Exercise Decreases Postmenopausal Bone Loss, Jameela Banu, Gabriel Fernandes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

There is increasing evidence suggesting that fish oil (FO) decreases bone resorption by reducing osteoclastogenesis and regular exercise (EX) increases bone mass. EX is associated with increasing bone formation. Therefore, the combined effects of FO intake and EX may have additive effects by both increasing bone formation and decreasing bone resorption. To demonstrate this, we studied the effects of FO and EX on the bone of mice after an ovariectomyinduced bone loss.

Twelve months old C57BL/6 female mice were either sham operated or ovariectomized, divided into different dietary and EX group and maintained for 3 months before sacrifice.

The distal …

Efectos Del Cambio Climático En La Interacción Planta-Hormiga: Una Mini Revisión Sobre La Tolerancia Térmica, Florencio Luna Castellanos, Mariana Cuautle, Teresa Patricia Feria-Arroyo, Citlalli Castillo Guevara

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Sin duda alguna el clima está cambiando debido al aumento de la concentración de gases de efecto invernadero en la atmósfera provocado por la acción humana. Dichos cambios van a tener un efecto importante en algunas actividades humanas tales como la agricultura y también pueden afectar enormemente la distribución geográfica de los organismos vivos y por tanto sus interacciones. Las hormigas y las plantas tienen una larga historia evolutiva de interacción. La actividad de las hormigas y su relación con las plantas en los últimos años ha tenido un número creciente de estudios que están poniendo de relieve su papel …

Sensory-Specific Satiety Is Intact In Rats Made Obese On A High-Fat, High-Sugar Choice Diet., Kevin P. Myers

Faculty Journal Articles

Sensory-specific satiety (SSS) is the temporary decreased pleasantness of a recently eaten food, which inhibits further eating. Evidence is currently mixed whether SSS is weaker in obese people, and whether such difference precedes or follows from the obese state. Animal models allow testing whether diet-induced obesity causes SSS impairment. Female rats (n = 24) were randomly assigned to an obesogenic high-fat, high-sugar choice diet or chow-only control. Tests of SSS involved pre-feeding a single palatable, distinctively-flavored food (cheese- or cocoa-flavored) prior to free choice between both foods. Rats were tested for short-term SSS (2 h pre-feeding immediately followed by 2 …

Current State Of Knowledge Regarding The Effects Of Dredging-Related ‘Pressure’ On Seagrasses: Report Of Theme 5 - Project 5.1.1 Prepared For The Dredging Science Node, Kathryn Mcmahon, Paul Lavery, John Statton, Roisin Mccallum, Udhi Hernawan

Research outputs 2014 to 2021

This review summarises our understanding, from a northwest of Western Australia and global perspective, the pressures seagrasses are exposed to from dredging, their tolerance thresholds and responses to dredging related stressors, and the bioindicators of dredging related stressors. From this information, we also identified gaps in our knowledge and areas where environmental management and monitoring approaches could be improved.

Aims

To determine the levels of stress (light reduction/sediment deposition) that occur under dredging conditions and evaluate the likely effect of these levels of pressure based on the existing seagrass literature, considering the range of variability and environmental quality conditions associated …

Genetic Variability Within Seagrass Of The North West Of Western Australia: Report Of Theme 5 - Project 5.2 Prepared For The Dredging Science Node, Kathryn Mcmahon, Udhi Hernawan, Kor-Jent Van Dijk, Michelle Waycott, Ed Biffin, Richard Evans, Paul Lavery

Research outputs 2014 to 2021

This study was the first of its kind to examine the patterns of genetic diversity in seagrasses in the Pilbara region of WA. Three species were assessed: Halophila ovalis (6 populations), Halodule uninervis (8 populations) andThalassia hemprichii (3 populations) at a range of spatial scales, within a meadow (centimetres−metres), among meadows at a local scale (2−60 km) and among meadows at a regional scale (up to 500 km). Due to the varied distribution of species all species across the same spatial scale and range of environments could not be sampled, so a nested approach was designed, with sites …

Seagrasses Of The North West Of Western Australia: Biogeography And Considerations For Dredging-Related Research: Report Of Theme 5 - Project 5.1.2 Prepared For The Dredging Science Node, Kathryn Mcmahon, John Statton, Paul Lavery

Research outputs 2014 to 2021

This review was undertaken to identify which seagrass primary producers (species or different functional groups) that collectively cover the bio-geographic range of key primary producers in the northwest of Western Australia are most appropriate for the focus of subsequent research into thresholds and indicators of response to dredging-related pressures (i.e. Theme 5). An assessment framework was developed based on six criteria: biogeographic range; ecological relevance (life-history strategy, habitats, natural dynamics and ecological services); current knowledge on thresholds and bioindicators; likelihood that species are sensitive and resilient to dredging related stressors; extent of improvement in applied knowledge; and likelihood of being …

Habitat Useage Of Tri-Colored Bats (Perimyotis Subflavus) In Western Kentucky And Tennessee Post-White Nose Syndrome, Katherine Schaefer

Murray State Theses and Dissertations

The tri-colored bat (Perimyotis subflavus) has declined severely across its range since 2006 due to white nose syndrome, a fungal disease causing massive bat mortality in North America. My objective was to determine distinguishing characteristics of roost trees and habitat used by tri-colored bats so that the species’ needs can be considered in management plans. I mist-netted for tri-colored bats in western Kentucky and Tennessee during the summers of 2015 and 2016, and attached a radio transmitter to each captured adult bat. I tracked 15 bats to their day roosts and collected habitat data at 38 roost areas …

Habitat Usage Of Tri-Colored Bats (Perimyotis Subflavus) In Western Kentucky And Tennessee Post-White Nose Syndrome, Katherine Schaefer

Murray State Theses and Dissertations

The tri-colored bat (Perimyotis subflavus) has declined severely across its range since 2006 due to white nose syndrome, a fungal disease causing massive bat mortality in North America. My objective was to determine distinguishing characteristics of roost trees and habitat used by tri-colored bats so that the species’ needs can be considered in management plans. I mist-netted for tri-colored bats in western Kentucky and Tennessee during the summers of 2015 and 2016, and attached a radio transmitter to each captured adult bat. I tracked 15 bats to their day roosts and collected habitat data at 38 roost areas …

Evaluating The Long Term Effects Of Continuous Broiler Litter Applications On Residual Soil Fertility And Yields Of Corn And Soybean Crops, And A Side Experiment Studying The Residual Copper Fertilization Effects On These Crops, Jesse Lee Gray Mr.

Murray State Theses and Dissertations

Abstract

The increasing cost of commercial fertilizers and the availability of animal waste that has high nutrient content have led to producers utilizing animal waste such as poultry litter in their fertility programs. This increased use has raised concerns as to what are the optimum rates to apply to meet crop needs and how will long term use at high rates of litter effect soil test levels of nutrients such as phosphorus and potassium. The objective of this study was to address these concerns. This study has been ongoing since 1997 with treatments of zero to seven tons of litter …

A Presence-Only Species Distribution Model Comparison Predicting The Distribution Of The Amphibian Chytrid Fungus (Batrachochytrium Dendrobatidis), Melanie L. Torres

Murray State Theses and Dissertations

Global anthropogenic impacts have incited devastating effects on both human and wildlife populations. Emerging infectious diseases (EID) are one consequence of these impacts. One particular EID, chytridiomycosis, is a threat to global amphibian populations, causing massive die-offs and several species extinctions. While predictive modeling has helped the general understanding of where the aquatic pathogen causing chytridiomycosis, Batrachochytrium dendrobatidis (Bd), could spread to, most models are on continental or countrywide scales. This coarse-scale modeling makes local management and conservation planning for imperiled amphibians difficult. In addition, modeling efforts can vary depending on location and species, making it necessary to test the …

Effects Of Posttranslational Modification Of Transcription Factor Gli-Similar 3 By Sumoylation On Insulin Transcription In Pancreatic Β Cells, Tyler M. Hoard

Murray State Theses and Dissertations

The ability to control blood glucose levels is a fundamental component of vertebrates. In these organisms, blood glucose homeostasis is achieved through a fine-tuned mechanism that largely involves the secretion of hormones from the endocrine pancreas into the bloodstream. These hormones include glucagon, which is secreted by the α cells of the pancreas and initiates the release of glucose into the bloodstream through gluconeogenesis in the liver, and insulin, which is secreted from the β cells and signals the uptake of excess blood glucose by the peripheral tissue. Gli-similar 3 (Glis3) is a transcription factor that has previously been shown …

Implementation Of A Flipped Classroom In A Non-Majors’ Biology Course, Leah Good

Murray State Theses and Dissertations

Changes to teaching practices have been requested in almost every field of science and mathematics but their implementation can be daunting. The flipped classroom has become a popular method in K-16 education for integrating active learning in the classroom. Research on the implementation of flipped classrooms has been met with mixed results, however. I sought to determine the effectiveness of the flipped classroom while addressing methodology needs cited by past studies including: using both faculty and student demographic variables, addressing assessment performance using concept inventories, and studying faculty who are not experts in education. I found that flipped and non-flipped …

A Performance Study Of Apis Mellifera With Dietary And Forage Restrictions During Spring Colony Establishment In Lyon County, Kentucky, Dominique Wood

Murray State Theses and Dissertations

One-third of our worldwide fruit, nut and vegetable production is completely dependent on the existence of pollinators. Commercial honey bees, Apis mellifera, have an annual economic value of $15 billion in the U.S. Additionally, their honey is valued at $150 million annually. In Fall 2006, commercial beekeepers observed sudden mass disappearances of whole colonies. By Spring 2007, the condition, dubbed Colony Collapse Disorder (CCD), raised environmental and fiscal concerns while the cause(s) continued to be sought. Forensic examinations of hive samples did not reveal a singular cause of CCD. The results pointed to a collection of detrimental factors affecting …

Soybean Cyst Nematode Populations As Influenced By A Non-Treated Soybean Crop, A Fluopyram-Treated Soybean Crop And A Non-Host Corn Crop, Margaret E. Lawrence

Murray State Theses and Dissertations

Soybean cyst nematodes are the most significant plant pathogen to soybeans in all soybean producing areas in the United States. Rotating to non-host crops, and using resistant varieties have been the main ways of managing this plant pathogen throughout the history of soybeans in this country. Fluopyram is a seed treatment that is labelled for the control of early season plant parasitic nematodes in soybean. Up until this point, there has been little to no published research conducted on the effectiveness of fluopyram against soybean cyst nematode. For this study, the objective was to conduct research to determine whether fluopyram …

The Effects Of Reward-Based Training On The Behavioral Assessment Of The Domestic Dog, Shelby Vandergraff, Shelby K. Vandergraff

Murray State Theses and Dissertations

Contrary to popular belief, pet overpopulation is caused more by owners failing to keep their dog than with accidental or intentional breeding (Moulton et al., 1991). Factors related to relinquishment have been researched, and concluded that behavioral problems are the most reoccurring reasons observed (Bailey, 1992; Miller at al., 1996; Patronek et al., 1996; Serpell, 1966; Salman et al., 1998, 2000; Scarlett et al, 1999; New et al., 2000; Shore et al., 2003, 2005; Mondelli 2004, Blackwell et al., 2008). When animals are relinquished to the shelter, the likelihood of the animal being euthanized increases. The purpose of this research …

Stage-Specific Timing Of The Microrna Regulation Of Lin-28 By The Heterochronic Gene Lin-14 In Caenorhabditis Elegans, Jennifer Tsialikas, Mitchell A. Romens, Allison L. Abbott, Eric G. Moss

Biological Sciences Faculty Research and Publications

In normal development, the order and synchrony of diverse developmental events must be explicitly controlled. In the nematode Caenorhabditis elegans, the timing of larval events is regulated by hierarchy of proteins and microRNAs (miRNAs) known as the heterochronic pathway. These regulators are organized in feedforward and feedback interactions to form a robust mechanism for specifying the timing and execution of cell fates at successive stages. One member of this pathway is the RNA binding protein LIN-28, which promotes pluripotency and cell fate decisions in successive stages. Two genetic circuits control LIN-28 abundance: it is negatively regulated by the miRNA …

Effects Of Fatigue And Recovery On Contractile Properties Of Frog Muscle, Robert H. Fitts, J. O. Holloszy

Biological Sciences Faculty Research and Publications

The contractile properties of frog sartorius muscles were evaluated during development of fatigue and recovery. Muscles were stimulated under anaerobic conditions at a rate of 30 stimuli/min. Twitch tension (P_t) decreased 59% and tetanic tension (P_o) decreased 52% during 15 min of stimulation. Contraction time and one-half relaxation time increased markedly during the first 6 min and then leveled off. The peak rate of twitch tension development (dP/dt) progressively declined to 33% of its initial value. Maximum shortening velocity did not change significantly. Large increases in P_t and twitch dP/dt occurred during …

Hopeful Monsters - In Defense Of Quests To Rediscover Long-Lost Species, David M. Watson, Robert A. Davis

Research outputs 2014 to 2021

Wild goose chase, snipe hunt, fool’s errand—these retorts typify responses of many biologists to news that someone is searching for a species considered extinct. Although these ventures can damage reputations and may offer false hope regarding the finality of extinction, genuine conservation gains often result, even for those quests that prove unsuccessful. As well as enhanced protection for regions where rediscovered species persist and new information of direct management relevance for co-occurring species, well-planned searches for long-lost species represent valuable engagement opportunities to raise awareness in the wider community about biodiversity conservation and science generally. Indeed, we suggest that “Lazarus …

The Highs And Lows Of Making A Bucket List - Quantifying Potential Mosquito Breeding Habitats In Metropolitan Backyards, Ram Sharan Lamichhane, Peter J. Neville, Jacques Oosthuizen, Kim Clark, Maria Fatouros, Shelley Beatty

Research outputs 2014 to 2021

While the development of land for residential housing along the Swan and Canning Rivers in Perth, WA, Australia has reduced natural mosquito breeding sites, the role of backyard container breeding remains a relatively unknown factor. Local Governments responsible for these areas focus management and control efforts on low lying, tidally driven mosquito habitats to control Aedes vigilax (Skuse) and Aedes camptorhynchus (Thomson) mosquitoes in an effort to reduce both the nuisance and disease risk to residents. In spite of their efforts, Local Governments continue to receive complaints regarding mosquito nuisance, even when environmental conditions do not favor hatching and development …

Disturbance Is An Important Driver Of Clonal Richness In Tropical Seagrasses, Kathryn M. Mcmahon, Richard D. Evans, Kor Jent Van Dijk, Udhi E. Hernawan, Gary A. Kendrick, Paul S. Lavery, Ryan J. Lowe, Marji L. Puotinen, Michelle Waycott

Research outputs 2014 to 2021

Clonality is common in many aquatic plant species, including seagrasses, where populations are maintained through a combination of asexual and sexual reproduction. One common measure used to describe the clonal structure of populations is clonal richness. Clonal richness is strongly dependent on the biological characteristics of the species, and how these interact with the environment but can also reflect evolutionary scale processes especially at the edge of species ranges. However, little is known about the spatial patterns and drivers of clonal richness in tropical seagrasses. This study assessed the spatial patterns of clonal richness in meadows of three tropical seagrass …

The Effects Of Aerobic Exercise And Lipolytic Stimulation On Skeletal Muscle And Adipose Expression Of Perilipin 3 (Plin3) In Individuals With Type 2 Diabetes And Women With Polycystic Ovary Syndrome, Jeffrey Daniel Covington

LSU Doctoral Dissertations

Ectopic lipid accumulation in skeletal muscle is linked with insulin resistance and type 2 diabetes (T2D). Neutral lipid is stored as lipid droplets, which are coated by lipid droplet coat proteins. One important class of these proteins are the perilipins. Little research has focused on perilipin 3 (PLIN3) and coatomer gtp-ases, theorized to be involved in PLIN3 directed lipid oxidation. Under lipolytic stimulation with epinephrine or palmitate, forskolin, and ionomysin (PFI), primary human myotubes taken from health non-T2D donors showed increased protein content levels of PLIN3. Concordantly, following endurance exercise in non-T2D males—an experimental paradigm shown to increase lipolysis—levels of …

Functional And Structural Analyses Of A Bacterial Antifreeze Protein, Chen Wang

LSU Doctoral Dissertations

Antifreeze proteins (AFPs) are a class of ice binding proteins (IBPs) that are expressed by different cold-adapted organisms to increase their freezing tolerance. AFPs have two major properties: thermal hysteresis and ice recrystallization inhibition. Here we report the functional and structural analyses of a bacterial AFP, IBPv. IBPv was originally secreted by a bacterium recovered from a deep glacial ice core drilled at Vostok Station, Antarctica. Our study showed that the recombinant protein rIBPv exhibited a thermal hysteresis of 2°C at concentrations higher than 50 µM, effectively inhibited ice recrystallization, and enhanced bacterial viability during freeze-thaw cycling. Circular dichroism scans …

Three Essays On Consumption Behavior, Credit, And Labor Supply Of Farm Households In Sub-Saharan Africa: Evidence From Uganda, Seydina Ousmane Sene

LSU Doctoral Dissertations

Consumption patterns, credit, and labor supply are determinants of human welfare, and also indicators of a country's economic progress, poverty, and inequalities. In this study, I review many of the stylized facts regarding consumption behaviors, credit, and the decision to engage in off-farm labor supply. I also put the life cycle model into an empirical model and perform testing. This is done in a three-essay format with a focus on the country of Uganda. In the first essay, I test whether farm households’ consumption behavior is consistent with an optimization process predicted by economic theory. In essay II, I evaluate …

The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende

LSU Doctoral Dissertations

The AVR4 effector, secreted by Cladosporium fulvum, has been demonstrated to be involved in pathogen virulence. Recent studies further demonstrated that Avr4 is highly conserved among several Cercospora species, indicating a potential important role of this gene in fungal virulence. Therefore, investigation to determine whether this fungal effector gene is present in Cercospora cf. flagellaris (previously known as C. kikuchii), the causal agent of soybean cercospora leaf blight (CLB) disease, and whether it plays any role in CLB disease development, is of great interest. In the present study, the Avr4 gene from C. cf. flagellaris was cloned and mutants lacking …

Organizational Misconduct Within The National Collegiate Athletic Association, Khirey Bennie Walker

LSU Doctoral Dissertations

Prior research on organizational misconduct within intercollegiate athletics focuses on the penalties administered by the NCAA and types of violations committed by member institutions, rather than the engagement of misconduct and the role of the NCAA as a social-control agent. Consequently, the purpose of this dissertation is to analyze the relationship between social-control agents and potential wrongdoers (i.e., organizations), in addition to how the relationship affects the evolving line separating right from wrong. A three-paper multiple method examination has been conducted, including a historical analysis of NCAA enforcement, a qualitative framing analysis on the detailing of NCAA major infractions to …

Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton

LSU Doctoral Dissertations

The twenty-first century has experienced a shift in cause of death worldwide from communicable diseases to noncommunicable diseases. Interestingly, many of these implicated chronic diseases, such as cancer, diabetes, and cardiovascular disease, have been shown to be programmed in the womb. As first posited by the Barker Hypothesis, adverse exposures in utero can increase an individual’s risk for chronic disease later in life. Therefore, pregnancy is an opportune time for intervention to improve the health of future generations. Studies of exposures known to negatively impact infant health, e.g. states of overnutrition (obesity, diabetes, excess gestational weight gain) and undernutrition (starvation, …

Exploring Social Indicators Of Situational Interest In Physical Education And Activity Classes: Integration Of Interest And Self-Determination Theories, Joseph Opiri Otundo

LSU Doctoral Dissertations

It is clearly documented that physically active lifestyles result in numerous health benefits. Unfortunately, a majority of individuals do not meet recommended physical activity (PA) guidelines. Physical education (PE) courses provide avenues for students to increase PA. Teachers and public health officials identify the importance of student motivation constructs such as interest in promoting PA behaviors and other healthy life habits. The purposes of this dissertation were to advance the measurement of situational interest by investigating social indicators derived from self-determination theory (SDT), and to test a theoretical model that integrates interest theory and SDT. Two quantitative studies were designed …

Evolutionary History Of A Large-Genome Salamander Across Five Disjunct Regions Of The Southeastern United States, Catherine Elizabeth Newman

LSU Doctoral Dissertations

The southeastern United States has a dynamic geologic history. Mountain range uplift, sea level fluctuations, and river basin evolution have shaped species geographic distributions in the region. My dissertation objectively identifies amphibian species with highly fragmented distributions in the Southeast (Chapter 2) and examines the phylogeographic history of one of those species, Plethodon serratus. This salamander species has a widely disjunct range across five regions: the Appalachians, the Ozarks, the Ouachitas, and two allopatric sites in Louisiana. Analyses of mitochondrial and nuclear loci (Chapter 3) showed that P. serratus is comprised of multiple genetic lineages, and the five regions are …

Evaluation Of A Role For Prolactin In The Recrudescense Of Ovarian Activity In Seasonally Anovulatory Mares, Erin Lea Oberhaus

LSU Doctoral Dissertations

A series of experiments studied the possible role for prolactin in the spring-time recrudescence of ovarian activity in mares. The first experiment was based on cabergoline administration to seasonally anovulatory mares to prevent the seasonal rise in endogenous plasma prolactin to assess whether reduced prolactin altered onset of ovarian activity or first ovulation. Although prolactin concentrations were reduced, basal prolactin concentrations recovered from suppression by the time of the next injection (10 days later), even though low-dose sulpiride stimulation of prolactin was still suppressed by 85%. The subsequent experiment tested whether season affected the duration and degree of suppression produced …

Epigenetic Profiling Of Mammalian Retrotransposons, Arundhati Bakshi

LSU Doctoral Dissertations

Over evolutionary time, mammalian genomes have accumulated a large number of retrotransposons, making up about half of the genome in any given species. These retrotransposons are typically repressed by epigenetic mechanisms, one of the main ones being DNA methylation. It is well known that improper DNA methylation of retrotransposons can have unwanted consequences on nearby gene expression, and hypomethylation of retrotransposons has been frequently observed in various cancers. Nevertheless, it has been notoriously difficult to study retrotransposon loci individually due to the highly repetitive nature of their sequences. To address this issue, we have developed a novel protocol termed HT-TREBS …