Life Sciences Commons^™

Distribution Of Rotifers Of High Mountain Lakes In The Eastern Black Sea Range Of Turkey, Di̇dem Özdemi̇r Mi̇s, Mustafa Ruşen Ustaoğlu

Turkish Journal of Zoology

Six expeditions were conducted in 2005-2007 on the rotifer fauna of 59 high mountain lakes of the Eastern Black Sea Range. All of the lakes are located at altitudes between 2530 and 3370 m except Uzungöl (1100 m). A total of 63 rotifer taxa were identified. All of the taxa that were identified are new records for their localities except Notholca squamula and Cephalodella gibba. Dissotrocha macrostyla, Hexarthra jenkinae, Lecane clara, Lecane kluchor, and Lecane latissima are new records for the Turkish inland water fauna. The trophic classification based on the orthophosphate includes 22 lakes as ultraoligotrophic and 37 lakes …

Identifying The Hotspots Of Wildlife-Vehicle Collision On The Çankırı-Kırıkkale Highway During Summer, Ali̇ Uğur Özcan, Nuri̇ Kaan Özkazanç

Turkish Journal of Zoology

In this study, we identified hotspots of mammal-vehicle collisions that occurred on the Çankırı-Kırıkkale highway between May and October 2014. We collected 58 records from 6 species. Sixteen casualties occurred on the part of the road with low traffic volume (1818 vehicles/day) and 42 casualties occurred on the part with moderate traffic volume (4680 vehicles/day). The two species with the highest number of records were hedgehog Erinaceus concolor (n = 27) and red fox Vulpes vulpes (n = 21). Hotspots of mammal-vehicle collisions were detected with the CrimeStat3 program using 750-m bandwidth. We identified two spots of high incidence of …

New Additions To Iranian Collembola (Entognatha: Hexapoda) From Ilam Province(Western Iran), Masoumeh Shayanmehr, Majid Mirabbalou, Igor J. Kaprus

Turkish Journal of Zoology

Three species of springtails were identified as new records for Iranian fauna from Ilam Province, western Iran: Hemisotoma quadrioculatus (Martynova, 1967), Isotoma anglicana Lubbock, 1862 (Isotomidae), and Metaphorura riozoi Castaño-Meneses, Palacios- Vargas & Traser, 2000 (Tullbergiidae). Brief information for each species about material examined, ecology, illustrations, and distribution is given.

Invertebrate Infestation In Green Turtle (Chelonia Mydas (Linnaeus, 1758)) Andloggerhead Turtle (Caretta Caretta (Linnaeus, 1758)) Nests On Alata Beach, Mersin, Turkey, Cemi̇l Aymak, Remzi̇ye Serap Ergene, Yusuf Katilmiş, Aşkin Hasan Uçar

Turkish Journal of Zoology

Invertebrate infestation in sea turtle nests (Caretta caretta and Chelonia mydas) was recorded for the first time for Alata beach, Mersin, Turkey. A total of 121 green and 32 loggerhead nests were recorded and 34 (22.22%) of these were examined in terms of the invertebrate faunal composition. The specimens found in the nests were identified to order, family, or genus levels and they were represented as 5 orders. These invertebrate groups are Pimelia sp. larvae (Tenebrionidae; Coleoptera), Elater sp. larvae (Elateridae; Coleoptera), Scarabaeidae larvae (Coleoptera), Muscidae (Diptera), Enchytraeidae (Oligochaeta), Myrmeleontidae (Neuroptera), and Cyrptostigmata (Acari).

Sex Differences In Chronic Stress Effects On Cognition In Rodents, Victoria N. Luine, Juan Gomez, Kevin D. Beck, Rachel E. Bowman

Psychology Faculty Publications

Chronic stress causes deleterious changes in physiological function in systems ranging from neural cells in culture to laboratory rodents, sub-human primates and humans. It is notable, however, that the vast majority of research in this area has been conducted in males. In this review, we provide information about chronic stress effects on cognition in female rodents and contrast it with responses in male rodents. In general, females show cognitive resilience to chronic stressors which impair male cognitive function using spatial tasks including the radial arm maze, radial arm water maze, Morris water maze, Y-maze and object placement. Moreover, stress often …

Redescription Of Euscorpius Tauricus (C.L. Koch, 1837), With The Description Of Two New Related Species From Greece (Scorpiones: Euscorpiidae), Gioele Tropea, Victor Fet, Aristeidis Parmakelis, Panayiota Kotsakiozi, Iasmi Stathi

Biological Sciences Faculty Research

Euscorpius tauricus (C. L. Koch, 1837) was previously known only from the Crimea Peninsula, Ukraine. We report an unexpected presence of this species in the Cyclades Islands (Greece) and northwestern Anatolia (Turkey). In addition we designate a neotype for this species. We synonymize Euscorpius carpathicus aegaeus Di Caporiacco, 1950 syn. n., from Antiparos Island and Euscorpius rahsenae Yağmur et Tropea, 2013 syn. n., from Anatolia, with E. tauricus. In addition, we describe two new species related to E. tauricus, from the Cyclades Islands: E. curcici sp. n., from Ios and Sikinos Islands, and E. amorgensis sp. n., from Amorgos Island. …

Effects Of High Intensity Ultrasound Frequency And High-Speed Agitation On Fat Crystallization, Roberta Silva, Juhee Lee, V. Gibon, Silvana Martini

Nutrition, Dietetics, and Food Sciences Faculty Publications

The objective of this research was to examine the effect of ultrasound frequency and high-speed agitation on lipid crystallization. Interesterified soybean oil was crystallized at 44 °C without and with the application of high intensity ultrasound (HIU—20 and 40 kHz) or with high-speed agitation (6000 and 24,000 rpm). Two tip amplitudes (24 and 108 µm) and three pulse durations were evaluated (5, 10, and 15 s) for the acoustic frequencies tested. Sonication at 20 kHz of frequency significantly reduced crystal size, increased (p < 0.05) elasticity (435.9 ± 173.3–80,218 ± 15,384 Pa) and SFC (0.2 ± 0.0–4.5 ± 0.4%). No significant difference was observed in the crystallization behavior of these samples when sonicated at different amplitudes for 5 and 10 s. The crystallization behavior was significantly delayed (p < 0.05) in samples sonicated using 108 µm amplitude for 15 s. Larger crystals were formed in samples sonicated at 40 kHz compared to those obtained with 20 kHz and lower SFC (3.7 ± 0.0%) and elasticity (3943 ± 1459 Pa) values were obtained. High-speed agitation at 24,000 rpm increased SFC (5.5 ± 0.1%) and crystallized area and decreased the elasticity (42,602 ± 11,775 Pa) compared to the samples sonicated at 20 kHz.

Sonocrystallization Of Interesterified Fats With 20 And 30% Of Stearic Acid At The Sn-2 Position And Their Physical Blends, Jeta V. Kadamne, Ebenezer A. Ifeduba, Casimir C. Akoh, Silvana Martini

Nutrition, Dietetics, and Food Sciences Faculty Publications

Physical blends (PB) of high oleic sunflower oil and tristearin with 20 and 30% stearic acid and their interesterified (IE) products where 20 and 30% of the fatty acids are stearic acid at the sn-2 position crystallized without and with application of high intensity ultrasound (HIU). IE samples were crystallized at supercooling temperatures (ΔT) of 12, 9, 6, and 3 °C while PB were crystallized at ΔT = 12 °C. HIU induced crystallization in PB samples, but not in the IE ones. Induction in crystallization with HIU was also observed at ΔT = 6 and 3 °C for IE C18:0 …

Quail 8: Proceedings Of The Eighth National Quail Symposium (July 26-28, 2017 : Knoxville, Tennessee), Thomas V. Dailey, Roger D. Applegate

Galliformes Specialist Group and Affiliated Societies: Conference Proceedings

In memoriam: Robert "Bob" J. Robel, 1933-2013

PLENARY

Translocation as a population restoration technique for northern bobwhites: A review and synthesis, James A. Martin, Roger D. Applegate, Thomas V. Dailey, Michelle Downey, Beth Emmerich, Fidel Hernández, Mark M. McConnell, Kelly S. Reyna, Dale Rollins, Rebekah E. Ruzicka, and Theron M. Terhune II

A focused habitat approach for northern bobwhite restoration in Kentucky, John J. Morgan, John M. Yeiser, Danna L. Baxley, Gary Sprandel, Ben A. Robinson, and Keith Wethington

Progress of the National Bobwhite Conservation Initiative, Donald F. McKenzie, John J. Morgan, and Thomas V. Dailey

The initial stages of …

Age Differences In Dynamic Fatigability And Variability Of Arm And Leg Muscles: Associations With Physical Function, Jonathon Senefeld, Tejin Yoon, Sandra K. Hunter

Exercise Science Faculty Research and Publications

Introduction

It is not known whether the age-related increase in fatigability of fast dynamic contractions in lower limb muscles also occurs in upper limb muscles. We compared age-related fatigability and variability of maximal-effort repeated dynamic contractions in the knee extensor and elbow flexor muscles; and determined associations between fatigability, variability of velocity between contractions and functional performance.

Methods

35 young (16 males; 21.0 ± 2.6 years) and 32 old (18 males; 71.3 ± 6.2 years) adults performed a dynamic fatiguing task involving 90 maximal-effort, fast, concentric, isotonic contractions (1 contraction/3 s) with a load equivalent to 20% maximal voluntary isometric …

Network Analytics For The Mirna Regulome And Mirna-Disease Interactions, Joseph Jayakar Nalluri

Theses and Dissertations

miRNAs are non-coding RNAs of approx. 22 nucleotides in length that inhibit gene expression at the post-transcriptional level. By virtue of this gene regulation mechanism, miRNAs play a critical role in several biological processes and patho-physiological conditions, including cancers. miRNA behavior is a result of a multi-level complex interaction network involving miRNA-mRNA, TF-miRNA-gene, and miRNA-chemical interactions; hence the precise patterns through which a miRNA regulates a certain disease(s) are still elusive. Herein, I have developed an integrative genomics methods/pipeline to (i) build a miRNA regulomics and data analytics repository, (ii) create/model these interactions into networks and use optimization techniques, motif …

Plaster Creek Bacterial Monitoring And Source Tracking Project - Final Report, Randall J. Dejong

Faculty and Professional Research

The objective of this project was to identify important sources of bacterial loading in Plaster Creek (Kent County). The following goals were set to reach this objective: 1) the relative contributions of bacterial loading for 10 major tributaries will be assessed and the five most egregious contributors will be prioritized; 2) molecular markers will be used to try to identify the primary taxonomic sources, and 3) to pinpoint bacterial loading locations using molecular markers and scent-trained canines in sub-watersheds.

Mechanisms Of Thai Medicinal Plant Extracts On The Attenuation Of Glutamate-Mediated Neurotoxicity, Anchalee Prasansuklab

Chulalongkorn University Theses and Dissertations (Chula ETD)

As the world population ages, neurodegenerative diseases, particularly Alzheimer's disease, are becoming a major public health concern with increasing incidence and prevalence worldwide while no cure currently exists. To challenge this situation, herbal medicine may provide a potential alternative treatment as use of natural-derived substances has been proven to be effective in the prevention and/or treatment of several diseases. During the past decades, increasing evidence has implicated excessive glutamate levels in the pathway of neuronal cell death. Thailand is a place known for cultivating a variety of tropical plants and herbs, so far many of them have never been examined …

Effects Of Plant Extracts On Melanin Biosynthesis And Melanogenetic Gene Expression In B16f10 Melanoma Cells, Moragot Chatatikun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Ultraviolet radiation from sunlight is a significant environmental factor in skin damage and can induce hyperpigmentation disorder and aesthetic problem. Development of novel whitening phytochemical compounds from natural products has been become trends recently. The purpose of this study was to find some plant extracts that reduce melanin synthesis and melanogenetic gene expression in alpha-MSH-induced B16F10 mouse melanoma cells. To screen total phenolics and flavonoids, antioxidant activity, and anti-mushroom tyrosinase activity, we used 13 plants which were extracted with petroleum ether, dichloromethane and ethanol solvents, subsequently. We found that total phenolic content of 13 plants extracts was found in the …

Role Of Progesterone Receptor A And B Isoforms In Lung Neuroendocrine Tumors, Teeranut Asavasupreechar

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung neuroendocrine tumors (NETs) incidences are increasing in recent years. Women with lung NETs have significantly better survival rates as compared to men suggesting the involvement of sex steroids and their receptors in the progression of lung NETs. Recent data suggested that progesterone receptor (PR) may play a role in the survival of lung NET patients. PR exists as two major isoforms, PRA and PRB. How expression of PR isoforms affects proliferation of lung NETs and patient's survival is not known. To determine the role of PR isoforms in lung NETs, we constructed H727 lung NET cell models expressing PRB …

Bioactive Clitoria Ternatea L. Flower Extract For The Glycemic Control To Carbohydrate Foods And Sugary Beverages, Charoonsri Chusak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Clitoria ternatea L. flower, an edible plant containing anthocyanins, has been currently used as the source of natural colorant in foods, beverages and desserts worldwide. The previous report demonstrated its biological properties including antioxidant and antihyperglycemic activity. However, the various concentrations of Clitoria ternatea were used in food and beverages but the effective concentration of Clitoria ternatea on the digestibility of starch and glycemic response remains unknown. In this study, the effects of foods and beverage containing Clitoria ternatea L. flower extract (CTE) on starch digestibility in vitro, postprandial glycemic response and antioxidant capacity in human as well as sensory …

The Effect Of Nutrition Education Intervention On The Amount Of School Lunch Consumption In Children Aged 3-5 Years: Case Study In Bangkok, Thailand, Hathaichanok Tirapongporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Low vegetable and fruit intake and micronutrient deficiency has been reported for Thai children aged three to five years. Many nutrition education programs have been recommended to solve these problems, especially for preschool children. This study aims to investigate the effect of the novel nutrition education program on the amount of vegetables, fruit, energy and nutrients consumption in school lunches of children aged three to five years. United for Healthier Kids is a nutrition education program that desires to motivate healthy eating via a set of nutrition education tools by encouraging variety in the types of food consumed, increase vegetables …

Effect Of Different Cooking Methods On The Bioaccessibility Of Polyphenols And Antioxidant Capacities Of Pakwan Ban (Sauropus Androgynus), Imas Arumsari

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study was aimed to assess the effect of different cooking methods on the amounts of phytochemical compounds and antioxidant capacities of sweet leaf (Sauropus androgynus) before and during in vitro simulated gastrointestinal digestion. The optimum cooking time of each cooking method was prior selected according to the total phenolic compounds, the ferric-reductase antioxidant power (FRAP) activity, the color characteristics, the hardness, and the texture preference of cooked sweet leaf samples. Consequently, the sweet leaf samples cooked by boiling for 5 mins or microwaving at 800 watts for 90 sec were compared with raw sweet leaf. The results showed that …

The Inhibitory Effect Of Anthocyanins-Rich Thai Berries Extracts On Lipid Digestion And Absorption, Netima Chamnansilpa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dyslipidemia is one of the major risk factor that contributes to chronic diseases such as cardiovascular disease and diabetic mellitus. Anthocyanin-rich Thai berries have been documented to have hypolipidemic activity. However, no scientific data is available regarding the mechanism of lipid lowering property. Thus, the objective of this study was to elucidate the mechanism of action of Thai berries including Prunus domestica L., Antidesma bunius (L.) Spreng, Syzygium cumini (L.) Skeels, Syzygium nervosum A. Cunn. Ex DC, and Muntingia calabura L. on a reduction of lipid digestion and absorption in vitro. Phytochemical compounds of Thai berries, including total phenolic (TP), …

Preparation And Antimicrobial Activity Of Olive Oil Nanoemulsions, Kusuma Jaemsak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to their health benefits including antioxidant, anti-inflammatory and also antimicrobial activities, extra virgin olive oil (EVOO) has received much attention in food, cosmetics, and pharmaceutical industries. Nanoemulsions (NEs) solubilize and increase absorption and bioavailability of lipophilic substances (i.e. olive oil). However, there is insufficient data in the formation of olive oil NEs. This study aimed to investigate the formation of oil-in-water NEs containing EVOO using low-energy approach and high-energy approach. NEs were characterized for size, polydispersity and zeta potential after storage at different conditions for up to 180 days. They were determined for antimicrobial susceptibility. From the result, NEs …

Relationship Between Depression And Metabolic Syndrome In Working Age Population In Bangkok, Matthawan Sujinnapram

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this observational descriptive cross-sectional study were to determine the prevalences of depression and metabolic syndrome and to examine the relationship between depression and metabolic syndrome and dietary pattern in working age people in Bangkok. The samples were 446 participants (123 males and 323 females) aged 20 and over who received an annual health examination 2017. The metabolic syndrome was defined by NCEP ATP III criteria. The data on waist circumference, fasting blood glucose, triglycerides, high-density lipoprotein cholesterol, blood pressure, depression score, and dietary pattern were collected. The relationship between depression and metabolic syndrome and dietary patterns were …

Microencapsulation Instant Coffee By Spray Drying Using Hydrolysed Konjac Glucomannan, Desi Sakawulan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research is aimed at finding a suitable wall material from konjac glucomannan to shield antioxidant compounds in coffee extract along with appropriate condition of spray drying to produce microencapsulated instant coffee. There are two steps of study. In the first step, two types of konjac powder (A. muelleri and A. bulbifer) and different concentrations of mannanase enzyme were used to investigate the viscosity reduction of konjac glucomannan hydrolysate (KGMH). It is found that mannanase at 38,000 units per gram substrate was able to lower the viscosity of A. muelleri solution to less than 100 m.Pa.s, while A. bulbifer remained …

Halophilic Bacteria From Salty Fermented Foods And Its Bacteriocin Encoding Gene Expression To Use As Biocontrol Agent Against Staphylococcus Aureus In Salt Added Foods, Vishal Chhetri

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research was aimed to isolate, select and apply bacteriocin producing halophilic bacteria from salty fermented foods, Plara (Thai traditional salty fermented fish) and soya sauce as bio-control agent in foods. The isolates having inhibitory activity against different strains of Staphylococcus aureus were selected, identified and characterized prior to application as protective starter in food models. Bacterial communities of Plara and soya sauce samples were studied by two methods, a cultural dependent and cultural independent method (Reverse Transcriptase PCR DGGE (Rev-T PCR-DGGE)), and subsequently sequenced by 16s rDNA analysis. Halanaerobium spp. in Plara and Staphylococcus gallinarum in soya sauce were …

Rapid Quality Determination Of Mulberry Leaf Tea During Storage Using Electronic Nose And Electronic Tongue, Anchalee Ruengdech

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study was aimed to develop rapid methods based on electronic nose (e-nose) and electronic tongue (e-tongue) to determine the quality attributes and phytochemicals of mulberry leaf tea. This study was divided into three parts: (1) to determine the effect of cultivar on the quality attributes and phytochemicals of mulberry leaf tea, (2) to evaluate the effect of packaging and storage time on quality attributes and phytochemicals of mulberry leaf tea, and (3) to employ e-tongue combined with chemometrics for prediction of phytochemicals and antioxidant activity of mulberry leaf tea. Mulberry leaf tea prepared from three different mulberry cultivars including …

Effects Of Processing Methods On Quality Of Green Coffee, Fareeya Kulapichitr

Chulalongkorn University Theses and Dissertations (Chula ETD)

To study the impact of processing on quality of Arabica green coffee, the research was divided into two parts: I) the study of the effects of postharvest processing practices on the quality of coffee, and II) the study of the effects of heat pump drying conditions on physicochemical properties of coffee with focus on the determination of the key volatile compounds affected by drying treatments. In part 1, the subtopics of the study were: 1.1) the study of the effects of the selection of coffee cherries differing in maturity (ripe vs unripe) on the quality of green coffee based on …

Use Of Near-Infrared Spectroscopy For Analysis Of Mixed Rice Bran And Parboiled Rice Bran, Wirongrong Maksawasd

Chulalongkorn University Theses and Dissertations (Chula ETD)

Both rice bran and parboiled rice bran can be used for rice bran oil production. Parboiled rice is more desirable than rice bran due to its higher oil content and lower acid value compared to rice bran, hence higher price. The difference in price of raw materials may motivate suppliers to mix raw rice bran in parboiled rice bran. As a result, rice bran oil manufacturers frequently come across parboiled rice bran samples that have higher acid value and lower oil content. Currently, Near Infrared Spectroscopy (NIR) is widely used in rice bran oil production plant as a rapid and …

Seasonal Variations Of Picophytoplankton, Nanophyto-Plankton And Microphytoplankton Communities At Sichang Island, Chonburi Province, Anassaya Deesuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sichang Island is in the eastern part of the Inner Gulf of Thailand where is primarily influenced by two monsoon winds, the northeast monsoon and the southwest monsoon. The effect of this factor lead to changes in phytoplankton community. However, most studies have been focused on microphytoplankton community not pico- and nanophytoplankton due to their small size and study methods. Therefore, seasonal variations of pico-, nano-, and microphytoplankton around Sichang Island were observed. The samplings were conducted at 10 stations around Sichang Island in two monsoon and two intermonsoon periods. Pico- and nanophytoplankton were analyzed by flow cytometry whereas microphytoplankton …

Effects Of Food On Growth And Gonadal Development Of Seahorse Hippocampus Sp., Thatpon Kamnurdnin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hippocampus sp. has received attention as a source of aquarium and ornamental trade. Seahorses have been threatened from non-target fisheries, habitat loss and traditional Chinese medicine (TCM). In response to concern about a decrease of population in the wild, an aquaculture of this species may provide the seahorse numbers for the commercial demands. However, one of the critical problems in aquaculture is the proper dietary for growth and their development. Therefore, this study was conducted on different types of preys on survival rates, growth rates and gonadal development in Hippocampus sp. during the day of birth until the 31day after …

Carotenoids And Metabolism Of The Green Microalga Chlorococcum Sp. 8367re Under Stress Conditions, Kantima Janchot

Chulalongkorn University Theses and Dissertations (Chula ETD)

Green microalgae are one of the natural resources for productions of various and beneficial bioactive compounds, such as UV-screening compounds and carotenoids. This study focuses on the changes of carotenoids and metabolisms in the green microalga Chlorococcum sp. 8367RE under stress conditions. The results showed that stresses by salt, light, drought, and nitrogen starvation affected on alterations of morphological characteristics. Interestingly, size of cells culturing under salt stresses dramatically increased. It should be noted that KCl stress caused the enlargement of cell sizes approximately 5 folds. Carotenoids were identified and quantified by using ultra-high performance liquid chromatography (UHPLC) and mass …

Functional Characterization Of Natural Sunscreen Compound Mycosporine-2-Glycine From Extremophilic Cyanobacterium Halothece Sp. Pcc 7418 In Macrophage Cell Line And Fresh Water Cyanobacterium, Supamate Tarasuntisuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycosporine-2-glycine (M2G) is a rare UV-screening compound in a group of mycosporine-like amino acids (MAAs). This molecule is found in only two cyanobacteria; Euhalothece sp. LK-1 and Aphanothece halophytica (Halothece sp. PCC 7418). The structure of M2G composed of 4-deoxygadusol as a core structure, attached by 2 molecules of glycine at C1 and C3 positions. M2G has a maximal adsorption at 331 nm and a better antioxidative activity than other MAAs. Thus, it is interesting in determination of other activities for further cosmeceutical and pharmaceutical applications. In this study, a high purity M2G was successfully extracted and purified from Halothece …