Life Sciences Commons^™

Redox Responsive Cerium Oxide Nanoparticles And Cd44 Targeted Nanomicelles For Selective Cancer Therapy, Zhaoxian Wang

Wayne State University Theses

Redox-responsive cerium oxide nanoparticles (CNPs) can play a versatile role in cancer therapy due to their ability to induce oxidative stress under proper pH conditions. Hyaluronic acid micelles with a high affinity to CD44 receptor which achieved more cytotoxicity towards cancer cells. The polymer-coated CNPs and HA-SMA-TPGS-CDF nanomicelles were developed for cancer therapy. The results showed that the toxicity of CNPs in tumor cells was mediated by the pro-oxidant property of CNPs under relative low pH conditions (pH~6.5) of the tumor microenvironment whereas CNPs play a (cytoprotective) anti-oxidant role, scavenging reactive oxygen species and reducing oxidative stress under normal pH …

Endocrine-Disrupting Properties Of Pharmaceuticals And Personal Care Products (Ppcps): An Evaluation Using Aquatic Model Organisms, Manahil Monshi

Wayne State University Theses

Thousands of chemicals have introduced into the environment as a result of human activity since the industrial revolution, and the U.S. Government Accountability Office has estimated that as many as 1,500 new chemical entities are synthesized each year ("Key Issues: Toxic Chemicals - High Risk Issue," 2017). Many of these chemicals are now found in surface water and ground water and can have detrimental effects on environmental health and on human health. This anthropogenic contamination has resulted in the labeling of the diverse array of chemicals found in water, which are not routinely monitored or regulated, as contaminants of emerging …

The Role Of Line-1 Transposable Element In Methamphetamine Neurotoxicity In The Neurogenic Zones, Dongyue Yu

Wayne State University Theses

Methamphetamine (METH) is a widely abused psychostimulant, which can cause neurotoxicity in the striatum and hippocampus. Several epigenetic changes were identified after binge METH exposure, including histone modification, DNA methylation, and changes in miRNA levels. We have shown that binge METH increases expression and activity of Long INterspersed Element (LINE-1), a transposable element, in doublecortin-positive neurons within rat neurogenic zones [1]. The goal of the present study was to identify which type(s) of cells show increases in LINE-1 following binge METH exposure, and determine whether binge METH-induced increases in LINE-1 are associated with cell death. To achieve this goal, male …

The Effect Of Oil Palm Phenolics (Opp) And Curcumin On Plasma Metabolomic Profile In Atherogenic Diet Induced Rat Model Of Alzheimer’S Disease (Ad), Soniya Katekar

Wayne State University Theses

Alzheimer's disease (AD) is the most widely recognized reason for dementia in the aging population. It is described by intellectual decay and deposition of ß-amyloid plaques in the hippocampus. It has been demonstrated that hypercholesterolemia incited by elevated cholesterol abstain from food is related with AD advancement. Expanded level of oxidative stress has additionally been seen in AD patients. An essential methodology to treat or postpone the disability depends on dietary change, utilizing nourishment supplements. OPP, a water-soluble fraction from oil palm fruit, rich in phenolics has been found to possess significant antioxidant activities. Curcumin, a polyphenol extricated from the …

National Franchise Members’ Perceptions Of The Exercise Psychosocial Environment, Ownership, & Satisfaction, E. Whitney G. Moore, Mary D. Fry

Kinesiology, Health and Sport Studies

The purpose of this study was to examine the relationship between: a) the motivational climate developed by a national exercise franchise and b) members’ sense of ownership in and enjoyment of exercise. Members of a national exercise franchise (N=5,329) were surveyed to learn their views on the motivational climate (autonomy supportive, caring, task- and ego-involving), exercise class ownership, and class enjoyment. Mean scale scores revealed this national exercise franchise succeeded in developing a high caring (M = 4.60 ± .68), task-involving (M = 4.04 ± .62), and autonomy supportive (M = 5.98 ± 1.00) climate, and low ego-involving (M = …

Western Bean Cutworm Infestation Rates In Relation To Their Location Of The Platte River, Blade Holmes

Undergraduate Research Journal

According to DuPont Pioneer, Western Bean Cutworm (WBCW) larval infestations can reduce the corn yield by 15-20%. Western Bean Cutworm can be found throughout the Corn Belt and are destructive because their feeding may cause a variety of molds to be introduced to the ear, which reduces the grain quality and negatively affects yield. Genes such as Herculex may provide suppression, but a timely insecticide treatment will provide more control of the WBCW.

Insecticide treatments can be applied aerially or by chemigation and are typically applied to prevent insect infestations or to control an infestation that is higher than desired. …

Data And Code For "Net Displacement And Temporal Scaling: Model Fitting, Interpretation, And Implementation", Garrett Street, Tal Avgar, Luca Börger

CFR Publications

No abstract provided.

Median Fin Patterning In Bony Fish: Caspase-3 Role In Fin Fold Reabsorption, Kaitlyn Ann Hammock

Undergraduate Honors Theses

Fish larvae develop a fin fold that will later be replaced by the median fins. I hypothesize that fin fold reabsorption is part of the initial patterning of the median fins, and that caspase-3, an apoptosis marker, will be expressed in the fin fold during reabsorption. I analyzed time series of larvae in the first 20-days post hatch (dph) to determine timing of median fin development in a basal bony fish - sturgeon- and in zebrafish, a derived bony fish. I am expecting the general activation pathway to be conserved in both fishes but, the timing and location of cell …

Pioglitazone, Neet Family Proteins, And Galactose Modulation Of Liver Cell Bioenergetics, David F. Grimm

Undergraduate Honors Theses

MitoNEET was discovered through interactions with a labeled and photoactive derivative of pioglitazone (pio), a drug used to increase peripheral insulin sensitivity. Its unique coordination of a [2Fe-2S] cluster by three cysteine residues (Cys-72, Cys-74, and Cys-83) and one histidine (His-87) gives this cluster both stability and the ability to be donated to acceptor proteins. These qualities allow mitoNEET to participate in a diversity of biological functions. Functions of mitoNEET and the consequences of pioglitazone (pio) treatment in human hepatocellular carcinoma (HepG2) cells cultured in glucose or galactose-based medium were examined by respiration and proliferation studies. Pio treatment decreased complex …

Molecular Modeling Of Novel Tryptamine Analogs With Antibiotic Potential Through Their Inhibition Of Tryptophan Synthase, Jared Schattenkerk

CMC Senior Theses

The growing prevalence of antibiotic-resistant bacteria is a global health crisis that threatens the effectiveness of antibiotics in medical treatment. Increases in the number of antibiotic-resistant bacteria and a drop in the pharmaceutical development of novel antibiotics have combined to form a situation that is rapidly increasing the likelihood of a post-antibiotic era. The development of antibiotics with novel enzymatic targets is critical to stall this growing crisis. In silico methods of molecular modeling and drug design were utilized in the development of novel tryptamine analogs as potential antibiotics through their inhibition of the bacterial enzyme tryptophan synthase. Following the …

Effects Of Grazers And Elevated Temperature On The Dynamics Of A Wastewater Algal Treatment System, Mengyuan Li

Theses and Dissertations

Wastewater algal treatment systems (ATS) have been shown to effectively remove excessive nutrients from wastewater and prevent eutrophication. However, the performance of ATS could be strongly affected by environmental factors. This study examined (1) the effect of grazers on nutrient removal rates and (2) the effect of 1.7 °C increase in temperature on algal biomass. The effect of grazers was assessed outdoors by analyzing dissolved and tissue nutrient concentrations in ATS with and without biological pesticide in two 20-day trials. The effect of elevated temperature was evaluated indoors by comparing algal biomass between heated and non-heated ATS for 20 days. …

Effects Of Estrogens On Astrocytes Exposed To Stressors, Bridget N. Smith

Theses and Dissertations

Hormone replacement therapy (HRT), used by many women to alleviate menopausal symptoms such as hot flashes and mood swings, is often a combination of horomones such as estrogens, progesterone, and conjugated equine estrogens (CEE), extracted from the urine of pregnant mares. Previous studies have found positive correlations between estradiol and cellular protection, but recent research has concluded CEE provide less protective mechanisms as compared to endogenous hormones. This research sought to compare the effects of estrogen treatments (single and combined estrogen) on viability when astrocytes were induced with stressors (epinephrine, cortisol, and low oxygen concentration). Cultured human astrocytes were treated …

Livestock-Livelihood Linkages In Uganda: The Benefits For Women And Rural Households?, Elizabeth Ransom, Carmen Bain, Iim Halimatusa'diyah

Sociology and Anthropology Faculty Publications

Livestock are an important component of rural households and gendered livelihood practices throughout sub-Saharan Africa. Widespread within the development literature is the belief in the livestock ladder, with poorer households often owning small stock and wealthier households owning large stock, with the assumption that poor households can utilize livestock to build their asset base and overtime this would allow poorer households to expand from small stock to large stock, in so doing climb the livestock ladder. There is also an assumption in the literature that women are more likely to oversee small stock. In addition, some well-known agricultural development programs …

Potassium Applications And Yellow Shoulder Disorder Of Tomatoes In High Tunnels, Elizabeth Maynard, Israel S. Calsoya, Jill Malecki

Midwest Vegetable Trial Reports

Tomatoes grown in unheated high tunnels can suffer significant loss in quality due to yellow shoulder disorder, particularly in susceptible varieties. This paper reports on a preliminary trial to determine whether incidence and severity of the disorder could be influenced by applying potassium fertilizers. Plots with higher leaf potassium content tended to have less fruit with severe yellow shoulder disorder, but increasing the amount of potassium fertilizer applied did not significantly reduce the disorder. From this study it isn’t possible to make specific recommendations for reducing yellow shoulder disorder with potassium applications.

Muskmelon Variety Trial In Southwest Indiana — 2016, Wenjing Guan, Dennis Nowaskie

Midwest Vegetable Trial Reports

No abstract provided.

2016 Evaluation Of Hybrid Bell Pepper Varieties For High Tunnel Production In Kansas, Kimberly Oxley, David Loewen, Cary L. Rivard

Midwest Vegetable Trial Reports

No abstract provided.

2016 Pumpkin Cultivar Evaluations In West Virginia, Lewis Jett

Midwest Vegetable Trial Reports

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 18 2017, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Letter From The Dean, Lona Robertson

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Message From The Faculty Editor, Mary Savin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

The Bovine Rumen Microbiome Revealed By Different Fractions Of Rumen Contents, Ashlee Breakstone, Jiangchao Zhao

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The bovine rumen microbiota is very important in terms of animal functionality and digestion. The fermentative capability of the rumen provides means for the digestion of complex plant material that is indigestible by humans. The rumen microbiota is made up of billions of microorganisms, primarily bacteria, that digest and ferment feed into volatile fatty acids and bacterial protein for the animal’s energy and protein needs, respectively. Changes to the rumen microbiota can have a direct measure on animal growth, health, and performance. The possibility of productivity boosts in the cattle industry make the rumen microbiome a hot topic in the …

Cost-Benefit Analysis Of A Genetic Marker On Cow-Calf Operations Differentiated By Pasture And Breed, Josh C. Crystal, Michael P. Popp, Nathan P. Kemper, Charles F. Rosenkrans Jr.

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Genetic sequencing in beef cattle (Bos taurus L.) is expected to aid producers with selecting breeding stock. Using data from experimental trials conducted with Angus, Brahman, and their reciprocal cross, the single nucleotide polymorphism (SNP) P450 C994G marker expression was investigated for use in selecting genetics suited to grazing endophyte-infected tall fescue (Festuca arundinacea Schreb. L.) compared to bermudagrass (Cynodon dactylon L.) pasture. The study is unique in the sense that actual cow-calf breeding failure rates (open cows were not culled) were tracked from 1991 to 1997 on herds that were bred to calf in spring and were either exposed …

Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleulin-8 Receptor Genes And Their Relationship To Production Traits And Hair Coat Scores In Crossbred Cattle, Avery B. Deaton, Laura Meyer, Jeremy Powell, Charles F. Rosenkrans Jr.

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Little is understood about how the diversity of genes, specifically the glucocorticoid receptor (GR) and interleukin-8 receptor (CXCR2), are related to reproductive health and how this affects physical traits in cattle. Glucocorticoid receptors have been positively associated with higher milk yields, lactose content, feed intake, and feed conversion rates. Interleukin-8 genes are part of the innate immune response and help with many aspects of female reproductive health, such as protecting the embryo from the maternal immune system during pregnancy. The objective of this research was to identify polymorphisms in the GR and CXCR2 genes and to associate genotypes between the …

An Assessment Of Economic Considerations For Industrial Hemp Production, Luke Lane, Jennie S. Popp, Michael P. Popp, Harrison M. Pittman

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

United States farm policy and programs are governed by the Farm Bill. The 2014 Farm Bill allows for the legal production and research of industrial hemp as long as it meets the standards outlined in the Farm Bill. Although it has a wide range of uses (upwards of 25,000 products use hemp), there is a lack of recent information regarding the economic feasibility of hemp production for the private agricultural sector. Through an extensive search of existing literature, information was gathered to construct an enterprise budget for industrial hemp. Data from the enterprise budget were used in a constrained linear …

Anti-Cancer And Bioavailabilty Of Arachidin-1 And Arachidin-3 In Colon Cancer Cells, Macy R. Shirley, Cindi Brownmiller, Wing Shun Lam, Sun-Ok Lee

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Cancer is a common cause of death in the United States and locally in the state of Arkansas. Modifiable factors such as tobacco use, physical activity, and diet lead to reduced incidence of colon cancer diagnosis. Plant-based foods may contain phytochemicals that confer health promoting properties. Specifically, peanuts contain phytochemicals known as resveratrol, arachidin-1, and arachidin-3 that have been linked to anticancer activities. However, few research studies have been done on arachidin-1 and arachidin-3 that could develop understanding of their health promoting properties or nutraceutical applications. The objectives of this study were to (1) determine the most effective concentration of …

Dental Biotribology: Final Thoughts And Future Directions, Zhongrong Zhou, Paul Constantino, Mark Hoffman, Mugino Kubo, Gildas Merceron, Mark Purnell, Mark F. Teaford

Faculty Publications & Research of the TUC College of Osteopathic Medicine

No abstract provided.

Contributions Of Myd88-Dependent Receptors And Cd11c-Positive Cells To Corneal Epithelial Barrier Function Against Pseudomonas Aeruginosa, Matteo M. E. Metruccio, Connie Tam, David J. Evans, Anna L. Xie, Michael E. Stern, Suzanne M. J. Fleiszig

Faculty Publications & Research of the TUC College of Pharmacy

Previously we reported that corneal epithelial barrier function against Pseudomonas aeruginosa was MyD88-dependent. Here, we explored contributions of MyD88-dependent receptors using vital mouse eyes and confocal imaging. Uninjured IL-1R (−/−) or TLR4 (−/−) corneas, but not TLR2 (−/−), TLR5 (−/−), TLR7 (−/−), or TLR9 (−/−), were more susceptible to P. aeruginosa adhesion than wild-type (3.8-fold, 3.6-fold respectively). Bacteria adherent to the corneas of IL-1R (−/−) or TLR5 (−/−) mice penetrated beyond the epithelial surface only if the cornea was superficially-injured. Bone marrow chimeras showed that bone marrow-derived cells contributed to IL-1R-dependent barrier function. In vivo, but not ex vivo …

Impact Of Dietary Resistant Starch On The Human Gut Microbiome, Metaproteome, And Metabolome, Tanja V. Maier, Marianna Lucio, Lang Ho Lee, Nathan C. Verberkmoes, Colin J. Brislawn, Jörg Bernhardt, Nathalie Bergeron

Faculty Publications & Research of the TUC College of Pharmacy

Diet can influence the composition of the human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on the functional potential of the microbiome. Dietary resistant starch (RS) has been shown to have health benefits, but we lack a mechanistic understanding of the metabolic processes that occur in the gut during digestion of RS. Here, we collected samples during a dietary crossover study with diets containing large or small amounts of RS. We determined the impact of RS on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” …

Benzylideneoxymorphone: A New Lead For Development Of Bifunctional Mu/Delta Opioid Receptor Ligands, Jason R. Healy, Padmavani Bezawada, Nicholas W. Griggs, Andrea L. Devereaux, Rae Reiko Matsumoto, John R. Traynor, Christopher W. Cunningham

Faculty Publications & Research of the TUC College of Pharmacy

Opioid analgesic tolerance remains a considerable drawback to chronic pain management. The finding that concomitant administration of delta opioid receptor (DOR) antagonists attenuates the development of tolerance to mu opioid receptor (MOR) agonists has led to interest in producing bifunctional MOR agonist/DOR antagonist ligands. Herein, we present 7-benzylideneoxymorphone (6, UMB 246) displaying MOR partial agonist/DOR antagonist activity, representing a new lead for designing bifunctional MOR/DOR ligands.