Life Sciences Commons^™

Elucidation Of Abscisic Acid In Stress Resistance Of Mnium Cuspidatum Through Salt Exposure And Chlorophyll Content Analysis, Kurt Corsbie

Undergraduate Honors Thesis Collection

Upon exposure to stresses, a battery of reactions occurs within the model moss Physcomitrella patens that induces the expression of defense-related genes. One of these reactions results in the production of abscisic acid (ABA), a plant hormone that helps a plant resist desiccation by inducing the activation of defense genes which results in protective physiological reactions. In this study, experiments were conducted on the moss species Mnium cuspidatum to see if it responds similarly to P. patens. This comparison was investigated as sample groups of M. cuspidatum and P. patens were pre-treated with 0µM, 50µM, or 500µM ABA to …

Dreadlocks (Dock) Is Necessary To Regulate Growth Of The Germline Ring Canals In The Developing Drosophila Melanogaster Egg Chamber, Olivia Crowe

Undergraduate Honors Thesis Collection

Infertility is a prevalent issue in the United States, impacting 1.5 million women (1). A possible cause of infertility is defects in gametogenesis, or the formation of sperm and egg. Therefore, understanding the basic mechanisms that promote normal gamete formation could impact our understanding of infertility. The Drosophila melanogaster egg develops from an organ-like structure called an egg chamber. The egg chamber is composed of a central cluster of 16 germ cells that are connected to one another by intercellular bridges, called ring canals. These ring canals are composed of filamentous actin and allow the transfer of materials from supporting …

Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …

Effective Use Of Probiotic-Glyconutrient Combination As An Adjuvant To Antibiotic Therapy For Diarrhea In Rearing Dairy Calves, Gilberto Lopez Valencia, Octavio Zapata-Ramirez, Lilia Núñez-González, Victor Núñez-Benítez, Heriberto Landeros- López, Maria Lopez-Soto, Alberto Barreras Serrano, Victor Gonzalez-Vizcarra, Alfredo Estrada-Angulo, Richard Zinn, Alejandro Plascencia

Turkish Journal of Veterinary & Animal Sciences

Thirty Holstein calves presented with infectious diarrhea during the second week following birth were utilized to evaluate the effects of a standardized product containing a combination of probiotics, ß-glucans, and glyconutrients (GLY) in support of antibiotic therapy during the early rearing phase (day 7 to day 45 following birth). Treatments were 1) antibiotic therapy (trimethoprim and sulfas; ANTIB), and 2) ANTIB plus 5 g/calf per day GLY. Treatments did not affect diarrhea duration, averaging 16.7 days. However, GLY support decreased (P < 0.05) the mortality rate and increased (P < 0.05) live weight gain. Increased weight gain was attributable to enhanced (P < 0.05) consumption of calf starter. Supplemental GLY increased (P < 0.05) serum glucose and lowered (P < 0.05) serum urea nitrogen, nonesterified fatty acids, and cortisol concentration. We conclude that daily provision of 5 g of a standardized product containing probiotics, ß-glucans, and glyconutrients to diarrheic calves under antibiotic therapy will promote health and enhance early growth performance.

Vegetation Response To Repeated Prescribed Burning And Varied Wildfire Severity In Upland Forests On The Cumberland Plateau, Kentucky, Zachary W. Poynter

Theses and Dissertations--Forestry and Natural Resources

As a result of decades of fire suppression, oaks (Quercus L.) and other disturbance-dependent tree species are experiencing widespread regeneration failure. Today, fire takes the form of relatively low to moderate intensity prescribed fire, used to restore fire adapted ecosystems, and wildfires which often vary in severity. I investigated long-term changes to forest structure and composition in response to repeated prescribed burning followed by an extended period of no fire. Burning reduced total basal area, midstory stem density and sapling stem density. However, the fire-free interval significantly increased sapling layer stem densities of oaks and competitor species. This research …

Guide To Quaking Aspen Ecology And Management With Emphasis On Bureau Of Land Management Lands In The Western United States, Paul C. Rogers

Aspen Bibliography

No abstract provided.

Genotypic Variability In Populus Tremula L. Affects How Anthropogenic Nitrogen Enrichment Influences Litter Decomposition, Franziska Bandau, Benedicte Riber Albrectsen, Riitta Julkunen-Tiitto, Michael J. Gundale

Aspen Bibliography

No abstract provided.

Can Carbon Fluxes Explain Differences In Soil Organic Carbon Storage Under Aspen And Conifer Forest Overstories?, Antra Boca, Helga Van Miegroet

Aspen Bibliography

No abstract provided.

Aspen Health On National Forests In The Northern Rocky Mountain Region, James T. Blodgett, Kurt K. Allen, Kendra Schotzko, Al Dymerski

Aspen Bibliography

No abstract provided.

Climatic Sensitivity Of A Mixed Forest Association Of White Spruce And Trembling Aspen At Their Southern Range Limit, Sophan Chhin, G. Geoff Wang

Aspen Bibliography

No abstract provided.

Post-Glacial Biogeography Of Trembling Aspen Inferred From Habitat Models And Genetic Variance In Quantitative Traits, Chen Ding, Stefan G. Schreiber, David R. Roberts, Andreas Hamann, Jean S. Brouard

Aspen Bibliography

No abstract provided.

Maintaining Ecosystem Resilience: Functional Responses Of Tree Cavity Nesters To Logging In Temperate Forests Of The Americas, Jose Tomas Ibarra, Michaela Martin, Kristina L. Cockle, Kathy Martin

Aspen Bibliography

Logging often reduces taxonomic diversity in forest communities, but little is known about how this biodiversity loss affects the resilience of ecosystem functions. We examined how partial logging and clearcutting of temperate forests influenced functional diversity of birds that nest in tree cavities. We used point-counts in a before-after-control-impact design to examine the effects of logging on the value, range, and density of functional traits in bird communities in Canada (21 species) and Chile (16 species). Clearcutting, but not partial logging, reduced diversity in both systems. The effect was much more pronounced in Chile, where logging operations removed critical nesting …

Quaking Aspen In The Northern Rockies Retention And Restoration, Camille Stevens-Rumann, Penelope Morgan, Eva Strand, Diane Abendroth

Aspen Bibliography

No abstract provided.

Assessment Of Aspen Ecosystem Vulnerability To Climate Change For The Uinta-Wasatch- Cache And Ashley National Forests, Utah, Janine Rice, Tim Bardsley, Pete Gomben, Dustin Bambrough, Stacey Weems, Allen Huber, Linda A. Joyce

Aspen Bibliography

No abstract provided.

Structural And Mechanistic Insights Into Hemoglobincatalyzed Hydrogen Sulfide Oxidation And The Fate Of Polysulfide Products, Victor Vitvitsky, Pramod K. Yadav, Sojin An, Javier Seravalli, Uhn-Soo Cho, Ruma V. Banerjee

Department of Biochemistry: Faculty Publications

Hydrogen sulfide is a cardioprotective signaling molecule but is toxic at elevated concentrations. Red blood cells can synthesize H₂S but, lacking organelles, cannot dispose of H₂S via the mitochondrial sulfide oxidation pathway. We have recently shown that at high sulfide concentrations, ferric hemoglobin oxidizes H₂S to a mixture of thiosulfate and iron-bound polysulfides in which the latter species predominates. Here, we report the crystal structure of human hemoglobin containing low spin ferric sulfide, the first intermediate in heme-catalyzed sulfide oxidation. The structure provides molecular insights into why sulfide is susceptible to oxidation in human …

Short Carboxylic Acid-Carboxylate Hydrogen Bonds Can Have Fully Localized Protons, Jiusheng Lin, Edwin Pozharski, Mark A. Wilson

Department of Biochemistry: Faculty Publications

Short hydrogen bonds (H-bonds) have been proposed to play key functional roles in several proteins. The location of the proton in short H-bonds is of central importance, as proton delocalization is a defining feature of low barrier hydrogen bonds (LBHBs). Experimentally determining proton location in H-bonds is challenging. Here, bond length analysis of atomic (1.15–0.98 Å) resolution X-ray crystal structures of the human protein DJ-1 and its bacterial homolog YajL was used to determine the protonation states of H-bonded carboxylic acids. DJ-1 contains a buried, dimer-spanning 2.49 Å H-bond between Glu15 and Asp23 that satisfies standard donor-acceptor distance criteria for …

1980 Lilley Cornett Woods Soil Data, Ryan W. Mcewan, Julia I. Chapman, Robert N. Muller

Data Files: Big Everidge Hollow Permanent Plots

Data collected in 1980 on the soil in the Big Everidge Hollow portion of the Lilley Cornett Woods Appalachian Ecological Research Station in southeastern Kentucky. Data key is provided in a supplemental file.

DOI: https://doi.org/10.26890/lcw.4b97vlypkk

1979 Lilley Cornett Woods Overstory Data, Ryan W. Mcewan, Julia I. Chapman, Robert N. Muller

Data Files: Big Everidge Hollow Permanent Plots

Data collected in 1979 on the overstory in the Big Everidge Hollow portion of the Lilley Cornett Woods Appalachian Ecological Research Station in southeastern Kentucky. Data key is provided in a supplemental file.

Accompanying photo by Julia I. Chapman.

DOI: https://doi.org/10.26890/lcw.s2qvt68sox

Development Of An Arab Food Frequency Questionnaire And Examination Of Changes In Dietary Patterns In Arab Immigrant Women To The United States, Samiah Alqahtani

Wayne State University Dissertations

INTRODUCTION: Immigrants to the U.S. face challenges and changes regarding to their food intake patterns that may be associated with their health status. However, less is known about the intake patterns among Arab immigrants.

OBJECTIVES 1: To establish a food intake database using 24-hr dietary records from Arab females who immigrated to the U.S. for less than 5 years, 5 to 10 years and greater than 10 years, as well as women still living in SA; 2: To establish an Arab Food Frequency Questionnaire (AFFQ) for future nutrition-related studies with Arab populations.

AIMS 1: To test the hypothesis that as …

Deubiquitinases In Ubiquitin Homeostasis And Disease, Gorica Ristic

Wayne State University Dissertations

Protein quality control (PQC) is indispensable for normal cellular functions, ensuring proteins are properly folded and removing those proteins that are functioning aberrantly. Perturbations in PQC can lead to various malignancies, neurodegeneration and neurological diseases. The Ubiquitin-Proteasome Pathway (UPP) is one important pathway in PQC, it relies on ubiquitination-dependent post-translational modification to selectively degrade misfolded or short-lived protein. Ubiquitination is reversed by the action of proteases known as deubiquitinating enzymes (DUBs). By hydrolyzing ubiquitin linkages, DUBs are responsible for cleaving and activating newly produced ubiquitin molecules, editing poly-Ub chains, removing ubiquitin from a substrate protein and recycling mono-Ub. Here, I …

Optimization Of Growth Conditions Of "Eubacterium Hallii" As A Potential Probiotics, Sampurna Guhathakurta

Wayne State University Theses

ABSTRACT

OPTIMIZATION OF GROWTH CONDITIONS OF “Eubacterium hallii” AS A POTENTIAL PROBIOTICS

by

SAMPURNA GUHATHAKURTA

May 2017

Advisor: Dr. Kequan Zhou

Major: Nutrition and Food Science

Degree: Master of Science

Probiotics are becoming popular to a greater extent over the past two decades for their potential benefits on human health. Therefore, they are widely used in the food industry by incorporation into food products. Nowadays, there are a lot of probiotics which are famous in the market. In this study, the strain of Eubacterium hallii was used because it has a significant importance in the pharmaceutical or food industry especially …

Protein Phosphatase 2a Function And Regulation In Insulin Resistant Human Skeletal Muscle Cells, Shukurat Sulaiman

Wayne State University Theses

Protein Phosphatase 2A (PP2A), a major serine/threonine phosphatase involved in insulin signaling pathway, plays a critical role in the development of insulin resistance and type 2 diabetes (T2D), which is characterized with hyperinsulinemic hyperglycemic condition. Moreover, majority of the glucose disposal takes place in the skeletal muscle which is also the main tissue responsible for insulin resistance. The catalytic subunit of PP2A (PP2Ac) can interact with multiple regulatory subunits and other regulatory proteins as well as substrates. These interactions are important for maintain normal PP2A function and subsequent cell signaling. We hypothesized that hyperinsulinemic hyperglycemic condition and insulin stimulation would …

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.

Aluminum And Phthalates In Calcium Gluconate: Contribution From Glass And Plastic Packaging, Robert A. Yokel, Jason M. Unrine

Pharmaceutical Sciences Faculty Publications

Introduction: Aluminum contamination of parenteral nutrition solutions has been documented for three decades. It can result in elevated blood, bone, and whole body aluminum levels associated with neurotoxicity, reduced bone mass and mineral content, and perhaps hepatotoxicity. The primary aluminum source among parenteral nutrition components is glass-packaged calcium gluconate, in which aluminum concentration the past three decades has averaged~ 4000 [mu]g/L, compared to < 200 [mu]g/L in plastic container-packaged calcium gluconate. A concern about plastic packaging is leaching of plasticizers, including phthalates, which have the potential to cause endocrine (male reproductive system) disruption and neurotoxicity.

Methods: Aluminum was quantified in samples collected periodically over more than two years from three calcium gluconate sources used to prepare parenteral nutrition solutions; two packaged in glass (from France and the US) and one in plastic …

Developmental Toxicity Of Nicotine: A Transdisciplinary Synthesis And Implications For Emerging Tobacco Products, Lucinda J. Enland, Kjersti Aagaard, Michele Bloch, Kevin Conway, Kelly Cosgrove, Rachel Grana, Thomas J. Gould, Dorothy Hatsukami, Frances Jensen, Denise Kandel, Bruce Lanphear, Frances Leslie, James R. Pauly, Jenae Neiderhiser, Mark Rubinstein, Theodore A. Slotkin, Eliot Spindel, Laura Stroud, Lauren Wakschlag

Pharmaceutical Sciences Faculty Publications

While the health risks associated with adult cigarette smoking have been well described, effects of nicotine exposure during periods of developmental vulnerability are often overlooked. Using MEDLINE and PubMed literature searches, books, reports and expert opinion, a transdisciplinary group of scientists reviewed human and animal research on the health effects of exposure to nicotine during pregnancy and adolescence. A synthesis of this research supports that nicotine contributes critically to adverse effects of gestational tobacco exposure, including reduced pulmonary function, auditory processing defects, impaired infant cardiorespiratory function, and may contribute to cognitive and behavioral deficits in later life. Nicotine exposure during …

Avoiding Proteolysis During Protein Purification, Barry J. Ryan, Gary T. Henehan

Books/Book Chapters/ Proceedings

All cells contain proteases which hydrolyze the peptide bonds between amino acids in a protein backbone. Typically, proteases are prevented from nonspecific proteolysis by regulation and by their physical separation into different subcellular compartments; however, this segregation is not retained during cell lysis, which is the initial step in any protein isolation procedure. Prevention of proteolysis during protein purification often takes the form of a two-pronged approach; firstly inhibition of proteolysis in situ, followed by the early separation of the protease from the protein of interest via chromatographical purification. Protease inhibitors are routinely used to limit the effect of the …

Progesterone Receptor And Prostaglandins Mediate Luteinizing Hormone-Induced Changes In Messenger Rnas For Adamts Proteases In Theca Cells Of Bovine Periovulatory Follicles, Erin L. Willis, Phillip J. Bridges, Joanne E. Fortune

Animal and Food Sciences Faculty Publications

Little is known about the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of extracellular proteases in ovarian follicles of non‐rodent species, particularly in theca cells. In the present study, temporal changes in the abundance of mRNA encoding four ADAMTS subtypes and hormonal regulation of mRNA encoding two subtypes were investigated in theca interna cells during the periovulatory period in cattle. Gonadotropin‐releasing hormone (GnRH) was injected into animals to induce a luteinizing hormone (LH)/follicle‐stimulating hormone (FSH) surge, and follicles were obtained at 0 hr post‐GnRH (preovulatory) or at 6, 12, 18, or 24 hr (periovulatory). ADAMTS1, ‐2, …

Where Policy And Practice Collide: Comparing Us,South African And European Union Approaches Toprotecting Children Online, Monica Bulger, Patrick Burton, Brian O'Neill, Elisabeth Staksrud

Articles

That children have a right to protection when they go online is an internationally well-established principle, upheld in laws that seek to safeguard children from online abuse and exploitation. However, children’s own transgressive behaviour can test the boundaries of this protection regime, creating new dilemmas for lawmakers the world over. This article examines the policy response from both the Global North and South to young people’s online behaviour that may challenge adult conceptions of what is acceptable, within existing legal and policy frameworks. It asks whether the ‘childhood innocence’ implied in much protection discourse is a helpful basis for promoting …

Study Of Phenolic Extractability In Grape Seeds By Means Of Atr-Ftir And Raman Spectroscopy, Julio Nogales-Bueno, Berta Baca-Bocanegra, Abigail Rooney, José Miguel Hernández-Hierro, Hugh Byrne, Francisco José Heredia

Articles

Near infrared hyperspectral imaging has been applied to grape seeds in order to select a representative subset of samples according to their spectral features in the 900-1700nm range. Afterwards, selected grape seeds have been classified according to their total phenol and flavanol extractabilities. In this way, samples were sorted in three different groups identified as low, medium and high extractability levels. In order to establish the chemical structures which can be responsible for the different extractabilities, vibrational spectroscopy has been applied to the non-extracted material after seed extractions. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) and Raman spectra of non-extracted …

Natural History Of The Peaks Of Otter Salamander (Plethodon Hubrichti) Along An Elevational Gradient, Timothy Brophy, Norman Reichenbach

Faculty Publications and Presentations

The Peaks of Otter salamander, Plethodon hubrichti, is a montane species found at elevations above 442 m within a 117 km2 area of the Blue Ridge Mountains in central Virginia, USA. In areas of this species’ range where the Eastern Red-backed salamander Plethodon cinereus, a potential competitor, is absent, surface-active salamander density was hypothesised to decrease at lower elevations due to increased temperatures and lower humidities, which may adversely affect salamander reproductive output and survival rates. Eggs/female, % gravid females, surface-active salamander density, temperature and relative humidity were recorded from nine sites ranging in elevation from 488 to 1143 m. …