Life Sciences Commons^™

Moderator Biographies, Heart Of America Grazing Conference

Kentucky Grazing Conference

No abstract provided.

Grazing Panel, Jeff Beasley, Trevor Toland, Ted Krauskopf

Kentucky Grazing Conference

No abstract provided.

Cover Crops/Annuals And Grazing, Roger Staff

Kentucky Grazing Conference

The use of annual forages (cover crops), both summer and winter, have been utilized for several years. When cover crops first were used to extend the grazing season, it began with one to three species in a mixture. That was before the soil health benefits were being observed and discussed as they are now. There have been a wide variety of planting methods used: airplanes, helicopters, conventional and No-till drills. Some seeding methods is simply broadcast on the soils surface and lightly tilled in or just rained in depending upon the weather. Then we discovered the herbicides and amounts used …

Mitigating The Challenges Of Grazing Lush, Spring Forages, W. Travis Meteer

Kentucky Grazing Conference

During the winter season most cattle are supplemented with dry forages, grains, and co-products. This ration is balanced and delivered to cattle. Then spring comes along and cattle are put out to grass. While green grass solves a lot of problems associated with winter feeding (manure, pen maintenance, calf health, and labor demands), it can pose nutritional challenges. Lush, spring forage has three major challenges when it comes to meeting cattle nutrition requirements.

Seedhead Suppression In Tall Fescue With Chaparral Herbicide, E. S. Flynn, P. B. Burch

Kentucky Grazing Conference

Tall Fescue (Lolium arundinaceum (Schreb.)) is cool-season perennial grass found in pastures throughout the Eastern US, most commonly in the transition zone. It’s tolerance to extreme temperatures, drought, poor soil fertility, heavy grazing, and pests have made it a reliable forage base for livestock pastures. Released in 1943, ‘KY-31’ tall fescue was quickly adopted by cattle producers and now inhabits an estimated 40 million acres in the US.

Soon after its adoption, cattle producers began to notice a reduction in animal performance and signs that animals were heat stressed following tall fescue consumption. These symptoms were an indication of an …

Fescue Tolerance Testing, Monty Kerley

Kentucky Grazing Conference

Fescue toxicosis is characterized by clinical symptoms such as vasoconstriction, immunosuppression, and poor thermoregulation. Fescue toxins bind to membrane receptors of cells that control constriction of blood through capillaries responsible for heat dissipation. When cattle consume toxic tall fescue, they lose ability to move blood to their skin where heat can be lost to the environment. As they lose this ability they become more prone to heat stress. In the winter, this lack of blood flow leads to other clinical symptoms, such as fescue foot and loss of tail switch, that occur from restricted blood flow.

Research Update: Beef Cattle Management Considerations For Grazing Tall Fescue, Frank Ireland

Kentucky Grazing Conference

It has been reported that 8 million beef cattle graze an estimated 35 million acres of endophyte-infected tall fescue in the United States with the majority of tall fescue being produced in the Midwest and southeastern regions of the United States. In the presence of the endophytic fungus, ergot alkaloids are present that when consumed by beef cattle results in decreased animal performance as measured in reduced weight gains, lower body condition scores, decreased conception rates, rough hair coats and the inability to regulate body temperatures. It has been estimated that this loss in animal performance accounts for a $600 …

In Defense Of Soil Health, Allen R. Williams

Kentucky Grazing Conference

Soil health is a hot topic today in sustainable agriculture circles and is even becoming more popular in conventional agriculture. However, the term is often thrown around loosely, and sometimes the meaning gets muddled.

Foreword And Conference Information [2017], Matt Bunger

Kentucky Grazing Conference

This is the front matter of the proceedings.

Origins: Life, The Universe And Everything Hpr 201, Michael Cerbo

Library Impact Statements

No abstract provided.

Quantifying The Impact Of Seasonal And Short-Term Manure Application Decisions On Phosphorus Loss In Surface Runoff, Peter A. Vadas, Laura Ward Good, William E. Jokela, Krishnapuram G. Karthikeyan, Francisco J. Arriaga, Melanie N. Stock

Plants, Soils, and Climate Faculty Publications

Agricultural phosphorus (P) management is a research and policy issue due to P loss from fields and water quality degradation. Better information is needed on the risk of P loss from dairy manure applied in winter or when runoff is imminent. We used the SurPhos computer model and 108 site–years of weather and runoff data to assess the impact of these two practices on dissolved P loss. Model results showed that winter manure application can increase P loss by 2.5 to 3.6 times compared with non-winter applications, with the amount increasing as the average runoff from a field increases. Increased …

Restoration Of The Iconic Pando Aspen Clone: Emerging Evidence Of Recovery, Paul C. Rogers, Jody A. Gale

Aspen Bibliography

Quaking aspen (Populus tremuloides Michx.) is being stressed across the America West from a variety of sources including drought, herbivory, fire suppression, development, and past management practices. Rich assemblages of plants and animals that utilize aspen forests, as well as economic values of tourism, grazing, hunting, and water conservation, make aspen ecosystems among the most valuable vegetation types in this region. The 43-ha Pando clone near Fish Lake, Utah, is an iconic example of an aspen community undergoing rapid decline due to overstory mortality and chronic recruitment failure. As part of a larger project to restore Pando, we fenced, …

Oviposition Traits Generate Extrinsic Postzygotic Isolation Between Two Pine Sawfly Species, Emily E. Bendall, Kim L. Vertacnik, Catherine R. Linnen

Biology Faculty Publications

Background: Although empirical data indicate that ecological speciation is prevalent in nature, the relative importance of different forms of reproductive isolation and the traits generating reproductive isolation remain unclear. To address these questions, we examined a pair of ecologically divergent pine-sawfly species: while Neodiprion pinetum specializes on a thin-needled pine (Pinus strobus), N. lecontei utilizes thicker-needled pines. We hypothesized that extrinsic postzygotic isolation is generated by oviposition traits. To test this hypothesis, we assayed ovipositor morphology, oviposition behavior, and host-dependent oviposition success in both species and in F1 and backcross females.

Results: Compared to N. lecontei, N. …

Identification, Validation, And Targeting Of The Mutant P53- Parp-Mcm Chromatin Axis In Triple Negative Breast Cancer, Wei-Gang Qiu, Alla Polotskaia, Gu Xiao, Lia Di, Yuhan Zhao, Wenwei Hu, John Philip, Ronald C. Hendrickson, Jill Bargonetti

Publications and Research

Over 80% of triple negative breast cancers express mutant p53. Mutant p53 often gains oncogenic function suggesting that triple negative breast cancers may be driven by p53 protein type. To determine the chromatin targets of this gain-of-function mutant p53 we used inducible knockdown of endogenous gain-of-function mtp53 in MDA-MB-468 cells in conjunction with stable isotope labeling with amino acids in cell culture and subcellular fractionation. We sequenced over 70,000 total peptides for each corresponding reciprocal data set and were able to identify 3010 unique cytoplasmic fraction proteins and 3403 unique chromatin fraction proteins. The present proteomics experiment corroborated our previous …

A Cognitive Electrophysiological Signature Differentiates Amnestic Mild Cognitive Impairment From Normal Aging, Juan Li, Lucas S. Broster, Gregory A. Jicha, Nancy B. Munro, Frederick A. Schmitt, Erin L. Abner, Richard J. Kryscio, Charles D. Smith, Yang Jiang

Behavioral Science Faculty Publications

Background: Noninvasive and effective biomarkers for early detection of amnestic mild cognitive impairment (aMCI) before measurable changes in behavioral performance remain scarce. Cognitive event-related potentials (ERPs) measure synchronized synaptic neural activity associated with a cognitive event. Loss of synapses is a hallmark of the neuropathology of early Alzheimer’s disease (AD). In the present study, we tested the hypothesis that ERP responses during working memory retrieval discriminate aMCI from cognitively normal controls (NC) matched in age and education.

Methods: Eighteen NC, 17 subjects with aMCI, and 13 subjects with AD performed a delayed match-to-sample task specially designed not only to be …

Corn Residue Use By Livestock In The United States, Marty R. Schmer, Rachael M. Brown, Virginia L. Jin, Robert B. Mitchell, Daren D. Redfearn

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) residue grazing or harvest provides a simple and economical practice to integrate crops and livestock, but limited information is available on how widespread corn residue utilization is practiced by US producers. In 2010, the USDA Economic Research Service surveyed producers from 19 states on corn grain and residue management practices. Total corn residue grazed or harvested was 4.87 million ha. Approximately 4.06 million ha was grazed by 11.7 million livestock (primarily cattle) in 2010. The majority of grazed corn residue occurred in Nebraska (1.91 million ha), Iowa (385,000 ha), South Dakota (361,000 ha), and Kansas …

Feeding Habits And Dietary Overlap Of Age-0 Winter Flounder (Pseudopleuronectes Americanus) And Summer Flounder (Paralichthys Dentatus) In Southern New England Tidal Rivers, David L. Taylor, Carissa L. Gervasi

Arts & Sciences Faculty Publications

Age-0 winter flounder (Pseudopleuronectes americanus; 20-90 mm in total length [TL]) and summer flounder (Paralichthys dentatus; 19-172 mm TL) were collected from the Seekonk and Taunton Rivers (in Rhode Island and Massachusetts, respectively) from May through September during 2009-2015, and stomach content analysis was used to assess diet composition and resource overlap for these species. Winter and summer flounder underwent ontogenetic dietary shifts. Winter flounder < 40 mm TL predominantly fed on copepods, transitioning to amphipods, isopods, and bivalves with increasing size. Polychaetes also were consumed frequently by winter flounder, irrespective of size. The principal prey of summer flounder < 60 mm TL were mysid shrimp and copepods, whereas sand shrimp (Crangon septemspinosa), amphipods, and fish were the dominant prey of larger conspecifics. There was minimal dietary overlap for the flounder species when comparisons were made independent of body size, indicating food niche segregation. For winter and summer flounder of equivalent sizes, however, dietary overlap was inversely related to TL. Moderate to high resource overlap occurred for small winter and summer flounder (< 40 mm TL) and was attributed to their mutual reliance on copepods and amphipods. Despite evidence of dietary overlap, it is unlikely that shared prey resources were diminished enough to negatively affect either flounder species.

Discovery Of Juniperus Sabina Var. Balkanensis R. P. Adams And A. N. Tashev In Western Turkey (Anatolia), Robert P. Adams, Adam Boratynski, Tuğrul Mataraci, Alexander N. Tashev, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications

Additional analyses of trnS-trnG and nrDNA from herbarium specimens from Europe revealed the presence of J. sabina var. balkanensis in western Turkey near Izmir and expands the range previously known only from Bulgaria and adjacent mountains in Greece. A more detailed map of the taxon's distribution is presented.

Dna Sequencing And Taxonomy Of Unusual Serrate Juniperus From Mexico: Chloroplast Capture And Incomplete Lineage Sorting In J. Coahuilensis And Allied Taxa, Robert P. Adams, M. Socorro Gonzalez-Elizondo, Martha Gonzalez-Elizondo, David Ramirez Noya, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications

Analysis of nrDNA, petN-psbM, trnS-trnG, trmD-trnT, and trnF-trnL of Juniperus coahuilensis and allied taxa of Mexico found typical J. coahuilensis, as well as individuals with: coahuilensis cp and hybrid ITS; coahuilensis cp and novel ITS sequence (La Parrilla type); novel Blue Fruited cp (blue fruited taxon) and coahuilensis ITS; plus Blue Fruited cp and La Parrila ITS. nrDNA data was examined and found to detect hybridization, chloroplast capture and incomplete lineage sorting. In addition, a new taxon was found with Blue Fruited (Blue Fruited) cp and J. martinezii ITS, suggestive of chloroplast capture. New records of J. saltillensis were confirmed …

Smokejumper Obituary: Haugland, Garth L. (Missoula 1959), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Cutler, Robert Eugene "Bob" (Missoula 1951), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Penetrance Estimates For Incidental Genomic Findings In Acmg-59, James A. Diao

Yale Day of Data

The dropping costs and rising popularity of next-generation sequencing has introduced the possibility of personalizing medical treatments and screening for genetic diseases. Still, the clinical community’s understanding remains incomplete, with limited consensus on the proper interpretation for many genetic variants. Thus, the standard procedure when returning sequencing results has been to report findings only in genes related to the diagnostic indication, and not incidental findings in other genes. To balance the threat of false positives with the medical benefits of true findings, the American College on Medical Genetics and Genomics (ACMG) recommends an exception: that clinical sequencing laboratories seek and …

Do Elephants Eat More Trees When Less Grass Is Available? A Field Study In Kruger National Park, South Africa., Emily Goldberg

Yale Day of Data

Although African bush elephants are often responsible for the majority of herbivore-driven savanna tree mortality, confusion remains regarding the factors that influence their diet. Some elephants either browse or graze almost exclusively, while others balance the two, and the determinants of this variation remain poorly understood. I seek to determine whether grass availability controls the proportion of woody plants in elephant diet and, therefore, the amount of damage elephants do to trees while foraging. Preliminary analysis using already-available data on grass biomass, elephant density, and elephant damage to trees suggest that tree damage is in fact negatively correlated with grass …

Symposium Volume 3, Issue 1

Symposium

Collection of research projects conducted by students of the College of Science and Math at California Polytechnic State University

Transcriptomic Response Of Wolf Spider, Pardosa Pseudoannulata, To Transgenic Rice Expressing Bacillus Thuringiensis Cry1ab Protein, Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song, Xuguo Zhou

Entomology Faculty Publications

Background: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice.

Results: Comparative transcriptome analysis resulted in 136 differentially expressed genes (DEGs) …

Crispr-Cas9 Mediated Nox4 Knockout Inhibits Cell Proliferation And Invasion In Hela Cells, Naser Jafari, Hyunju Kim, Rackhyun Park, Liqing Li, Minsu Jang, Andrew J. Morris, Junsoo Park, Cai Huang

Markey Cancer Center Faculty Publications

Increased expression of NOX4 protein is associated with cancer progression and metastasis but the role of NOX4 in cell proliferation and invasion is not fully understood. We generated NOX4 knockout HeLa cell lines using the CRISPR-Cas9 gene editing system to explore the cellular functions of NOX4. After transfection of CRISPR-Cas9 construct, we performed T7 endonuclease 1 assays and DNA sequencing to generate and identify insertion and deletion of the NOX4 locus. We confirmed the knockout of NOX4 by Western blotting. NOX4 knockout cell lines showed reduced cell proliferation with an increase of sub-G1 cell population and the decrease of S/G2/M …

Network Dynamics Mediate Circadian Clock Plasticity, Abdelhalim Azzi, Jennifer A. Evans, Tanya Leise, Jihwan Myung, Toru Takumi, Alec J. Davidson, Steven A. Brown

Biological Sciences Faculty Research and Publications

A circadian clock governs most aspects of mammalian behavior. Although its properties are in part genetically determined, altered light-dark environment can change circadian period length through a mechanism requiring de novo DNA methylation. We show here that this mechanism is mediated not via cell-autonomous clock properties, but rather through altered networking within the suprachiasmatic nuclei (SCN), the circadian “master clock,” which is DNA methylated in region-specific manner. DNA methylation is necessary to temporally reorganize circadian phasing among SCN neurons, which in turn changes the period length of the network as a whole. Interruption of neural communication by inhibiting neuronal firing …

U.S. Drought Monitor, January 17, 2017, Richard Tinker

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for January 17, 2017 (1/17/17) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

The Mayfly Newsletter, Donna J. Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Association Of Body Mass Index With Dna Methylation And Gene Expression In Blood Cells And Relations To Cardiometabolic Disease: A Mendelian Randomization Approach, Michael M. Mendelson, Riccardo E. Marioni, Roby Joehanes, Chunyu Liu, Åsa K. Hedman, Stella Aslibekyan, Ellen W. Demerath, Weihua Guan, Degui Zhi, Chen Yao, Tianxiao Huan, Christine Willinger, Brian Chen, Paul Courchesne, Michael Multhaup, Marguerite R. Irvin, Ariella Cohain, Eric E. Schadt, Megan L. Grove, Jan Bressler, Kari North, Johan Sundström, Stefan Gustafsson, Sonia Shah, Allan F. Mcrae, Sarah E. Harris, Jude Gibson, Paul Redmond, Janie Corley, Lee Murphy, Donna K. Arnett

Epidemiology and Environmental Health Faculty Publications

Background

The link between DNA methylation, obesity, and adiposity-related diseases in the general population remains uncertain.

Methods and Findings

We conducted an association study of body mass index (BMI) and differential methylation for over 400,000 CpGs assayed by microarray in whole-blood-derived DNA from 3,743 participants in the Framingham Heart Study and the Lothian Birth Cohorts, with independent replication in three external cohorts of 4,055 participants. We examined variations in whole blood gene expression and conducted Mendelian randomization analyses to investigate the functional and clinical relevance of the findings. We identified novel and previously reported BMI-related differential methylation at 83 CpGs …