Life Sciences Commons^™

Neurotensin Nts1 And Nts2 Receptor Agonists Produce Anxiolytic-Like Effects In The 22-Khz Ultrasonic Vocalization Model In Rats, Floyd F. Steele Iii, Shannon C. Whitehouse, Jacob S. Aday, Adam J. Prus

Journal Articles

Neurotensin is a neuropeptide neurotransmitter that interacts with multiple neurotransmitter systems, including those regulating amygdalar function, via NTS₁ and NTS₂ receptors. Both receptors are expressed in the amygdala and agonists for NTS₁ or NTS₂ receptors have exhibited anxiolytic effects in animal models. Systemic adminstration of NTS₁ receptor agonist PD149163 was recently shown to reduce footshock conditioned 22-kHz ultrasonic vocalizations in rats, suggesting that PD149163 produced an anxiolytic effect. The effects that neurotensin may have or a selective NTS₂receptor agonist may have on 22-kHz vocalizations has yet to be examined. The current study evaluated …

Neurotrophic Factors Ngf, Gdnf And Ntn Selectively Modulate Hsv1 And Hsv2 Lytic Infection And Reactivation In Primary Adult Sensory And Autonomic Neurons, Andy Y. Yanez, Telvin Harrell, Heather J. Sriranganathan, Angela M. Ives, Andrea S. Bertke

School of Medical Diagnostics & Translational Sciences Publications

Herpes simplex viruses (HSV1 and HSV2) establish latency in peripheral ganglia after ocular or genital infection, and can reactivate to produce different patterns and frequencies of recurrent disease. Previous studies showed that nerve growth factor (NGF) maintains HSV1 latency in embryonic sympathetic and sensory neurons. However, adult sensory neurons are no longer dependent on NGF for survival, some populations cease expression of NGF receptors postnatally, and the viruses preferentially establish latency in different populations of sensory neurons responsive to other neurotrophic factors (NTFs). Thus, NGF may not maintain latency in adult sensory neurons. To identify NTFs important for maintaining HSV1 …

Reduced Mitochondrial Efficiency Explains Mismatched Growth And Metabolic Rate At Supraoptimal Temperatures., Eloy Martinez, Michael A. Menze, Salvatore J. Agosta

Faculty and Staff Scholarship

The relationship between whole-organism growth and metabolism is generally assumed to be positive and causative; higher metabolic rates support higher growth rates. In Manduca sexta, existing data demonstrate a deviation from this simple prediction: at supraoptimal temperatures for larval growth, metabolic rate keeps increasing while growth rate is decreasing. This mismatch presumably reflects the rising “cost of maintenance” with temperature. Precisely what constitutes this cost is not clear, but we suspect the efficiency with which mitochondria harness oxygen and organic substrates into cellular energy (ATP) is key. We tested this by integrating existing data on M. sexta growth and …

Measuring Extension Program Impacts: 1. Setting Goals And Objectives, Mark Larese-Casanova

All Current Publications

This fact sheet descries the first step in measuring impacts of Extension programs, goals and objectives and includes defining measurable objectives and developing activities.

The Effects Of Xylanase Or An Algal Sourced Zinc Polysaccharide Complex On The Performance Of Laying Hens Fed A Corn And Soybean Diet, Ashley Bigge

Department of Animal Science: Dissertations, Theses, and Student Research

Two studies were conducted to determine the effects of two different feed additives on the performance of laying hens. Study 1 examined the effects of non-starch polysaccharide degrading enzyme inclusion in a low energy diet. Study 2 examined the effects of supplementing a paramylon zinc polysaccharide complex at increasing concentrations. Both studies utilized a randomized complete block design for treatment assignment, and data were analyzed using the Glimmix procedure in SAS 9.4 for windows.

The first study took place July 2014 to March 2015 and consisted of two phases. Each phase tested a positive control, negative control with lower ME …

Krillbase: A Circumpolar Database Of Antarctic Krill And Salp Numerical Densities, 1926–2016, Angus Atkinson, Siemon L. Hill, Evgeny A. Pakhomov, Volker Siegel, Ricardo Anadon, Sanae Chiba, Kendra L. Daly, Rod Downie, Sophie Fielding

Marine Science Faculty Publications

Antarctic krill (Euphausia superba) and salps are major macroplankton contributors to Southern Ocean food webs and krill are also fished commercially. Managing this fishery sustainably, against a backdrop of rapid regional climate change, requires information on distribution and time trends. Many data on the abundance of both taxa have been obtained from net sampling surveys since 1926, but much of this is stored in national archives, sometimes only in notebooks. In order to make these important data accessible we have collated available abundance data (numerical density, no. m⁻² ) of postlarval E. superba and salp individual (multiple …

A Walk On The Wild Side: Disturbance Dynamics And The Conservation And Management Of European Mountain Forest Ecosystems, Dominik Kulakowski, Rupert Seidl, Jan Holeksa, Timo Kuuluvainen, Thomas A. Nagel, Momchil Panayotov, Miroslav Svoboda, Simon Thorn, Giorgio Vacchiano, Cathy Whitlock, Thomas Wohlgemuth, Peter Bebi

Geography

Mountain forests are among the most important ecosystems in Europe as they support numerous ecological, hydrological, climatic, social, and economic functions. They are unique relatively natural ecosystems consisting of long-lived species in an otherwise densely populated human landscape. Despite this, centuries of intensive forest management in many of these forests have eclipsed evidence of natural processes, especially the role of disturbances in long-term forest dynamics. Recent trends of land abandonment and establishment of protected forests have coincided with a growing interest in managing forests in more natural states. At the same time, the importance of past disturbances highlighted in an …

Conservation Status Of The American Horseshoe Crab, (Limulus Polyphemus): A Regional Assessment, David R. Smith, H. Jane Brockmann, Mark Beekey, Timothy L. King, Michael J. Millard, Jaime Zaldívar-Rae

Biology Faculty Publications

Horseshoe crabs have persisted for more than 200 million years, and fossil forms date to 450 million years ago. The American horseshoe crab (Limulus polyphemus), one of four extant horseshoe crab species, is found along the Atlantic coastline of North America ranging from Alabama to Maine, USA with another distinct population on the coasts of Campeche, Yucatán and Quintana Roo in the Yucatán Peninsula, México. Although the American horseshoe crab tolerates broad environmental conditions, exploitation and habitat loss threaten the species. We assessed the conservation status of the American horseshoe crab by comprehensively reviewing available scientific information on its range, …

Temperature-Dependent Phenology Of Plutella Xylostella (Lepidoptera: Plutellidae): Simulation And Visualization Of Current And Future Distributions Along The Eastern Afromontane, Benignus V. Ngowi, Henri E.Z. Tonnang, Evans M. Mwangi, Tino Johansson, Janet Ambale, Paul N. Ndegwa, Sevgan Subramanian

All Peer-Reviewed Publications

There is a scarcity of laboratory and field-based results showing the movement of the diamondback moth (DBM) Plutella xylostella (L.) across a spatial scale. We studied the population growth of the diamondback moth (DBM) Plutella xylostella (L.) under six constant temperatures, to understand and predict population changes along altitudinal gradients and under climate change scenarios. Non-linear functions were fitted to continuously model DBM development, mortality, longevity and oviposition. We compiled the best-fitted functions for each life stage to yield a phenology model, which we stochastically simulated to estimate the life table parameters. Three temperature-dependent indices (establishment, generation and activity) were …

Marine Aquarium Trade In India: Challenges And Opportunities For Conservation And Policy, Sanjeevi Prakash, Thipramalai Thangappan Ajith Kumar, Rajeev Raghavan, Andrew L. Rhyne, Michael F. Tlusty, Thanumalaya Subramoniam

Arts & Sciences Faculty Publications

The collection of marine taxa for the aquarium trade continues to demand live animals be extracted from reefs, but in doing so, offers economic benefits for local communities. To improve our understanding of the status of marine aquariumtrade in India, information on harvested species and their volume was gathered at the major collection hubs (Tuticorin, Kilakarai and Mandapam) in the Gulf of Mannar region, and compared to the export data. During one year, 87 species of fish (51% belonging to the family Pomacentridae) and 21 species of invertebrates were harvested for the trade. The conservation status of exploited species revealed …

The Role Of Language In Anatomy And Physiology Instruction, Angela M. Chapman, Hsuying C. Ward, Ashwini Tiwari, Amy Weimer, Jaime B. Duran, Federico R. Guerra, Robert Paul Sale

Teaching and Learning Faculty Publications

Research indicates that student learning of science, student attitudes toward science, and their motivation to learn science and pursue science-related careers is related to classroom instruction. This study examined anatomy and physiology (A&P) classes in a south Texas high school where 97 percent of students are Hispanic bilingual learners. Classes were assigned to control or treatment groups, with the treatment group receiving instruction designed to help students develop a deeper understanding of anatomy vocabulary related to brain structures by making connections to these words in everyday life as well as to their understanding of Spanish. Main effects between group and …

Sequential Displacement Of Type Vi Secretion System Effector Genes Leads To Evolution Of Diverse Immunity Gene Arrays In Vibrio Cholerae, Paul C. Kirchberger, Daniel Unterweger, Daniele Provenzano, Stefan Pukatzki, Yan Boucher

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Type VI secretion systems (T6SS) enable bacteria to engage neighboring cells in contact-dependent competition. In Vibrio cholerae, three chromosomal clusters each encode a pair of effector and immunity genes downstream of those encoding the T6SS structural machinery for effector delivery. Different combinations of effector-immunity proteins lead to competition between strains of V. cholerae, which are thought to be protected only from the toxicity of their own effectors. Screening of all publically available V. cholerae genomes showed that numerous strains possess long arrays of orphan immunity genes encoded in the 3' region of their T6SS clusters. Phylogenetic analysis reveals that these …

Two Adjacent Phenylalanines In The Nmda Receptor Glun2a Subunit M3 Domain Interactively Regulate Alcohol Sensitivity And Ion Channel Gating, Hong Ren, Yulin Zhao, Man Wu, Donard S. Dwyer, Robert W. Peoples

Biological Sciences Faculty Research and Publications

The N-methyl-d-aspartate (NMDA) receptor is a key target of ethanol action in the central nervous system. Alcohol inhibition of NMDA receptor function involves small clusters of residues in the third and fourth membrane-associated (M) domains. Previous results from this laboratory have shown that two adjacent positions in the M3 domain, F636 and F637, can powerfully regulate alcohol sensitivity and ion channel gating. In this study, we report that these positions interact with one another in the regulation of both NMDA receptor gating and alcohol action. Using dual mutant cycle analysis, we detected interactions among various substitution mutants at these …

Identification And Characterization Of Microrna Differentially Expressed In Macrophages Exposed To Porphyromonas Gingivalis Infection, Olivier Huck, J Al-Hashemi, L Poidevin, O Poch, J Davideau, H Tenenbaum, Salomon Amar

NYMC Faculty Publications

MicroRNAs (miRNAs) are short, noncoding RNAs involved in the regulation of several processes associated with inflammatory diseases and infection. Bacterial infection modulates miRNA expression to subvert any innate immune response. In this study we analyzed, using microarray analysis, the bacterial modulation of miRNAs in bone marrow-derived macrophages (BMMs) in which activity was induced by infection with Porphyromonas gingivalis The expression of several miRNAs was modulated 3 h postinfection (at a multiplicity of infection of 25). A bioinformatic analysis was performed to further identify pathways related to the innate immune host response under the influence of selected miRNAs. To assess the …

Phenotypically Heterogeneous Podoplanin-Expressing Cell Populations Are Associated With The Lymphatic Vessel Growth And Fibrogenic Responses In The Acutely And Chronically Infarcted Myocardium, M Cimini, A Cannata, G Pasquinelli, Marcello Rota, P Goichberg

NYMC Faculty Publications

Cardiac lymphatic vasculature undergoes substantial expansion in response to myocardial infarction (MI). However, there is limited information on the cellular mechanisms mediating post-MI lymphangiogenesis and accompanying fibrosis in the infarcted adult heart. Using a mouse model of permanent coronary artery ligation, we examined spatiotemporal changes in the expression of lymphendothelial and mesenchymal markers in the acutely and chronically infarcted myocardium. We found that at the time of wound granulation, a three-fold increase in the frequency of podoplanin-labeled cells occurred in the infarcted hearts compared to non-operated and sham-operated counterparts. Podoplanin immunoreactivity detected LYVE-1-positive lymphatic vessels, as well as masses of …

Pnaktide Attenuates Steatohepatitis And Atherosclerosis By Blocking Na/K-Atpase/Ros Amplification In C57bi6 And Apoe Knockout Mice Fed A Western Diet, Komal Sodhi, K Srikanthan, P Goguet-Rubio, A Nichols, A Mallick, A Nawab, R Martin, P Shah, M Chaudhry, S Sigdel, M El-Hamdani, J Liu, Z Xie, Nader Abraham, J Shapiro

NYMC Faculty Publications

We have previously reported that the alpha1 subunit of sodium potassium adenosine triphosphatase (Na/K-ATPase), acts as a receptor and an amplifier for reactive oxygen species, in addition to its distinct pumping function. On this background, we speculated that blockade of Na/K-ATPase-induced ROS amplification with a specific peptide, pNaKtide, might attenuate the development of steatohepatitis. To test this hypothesis, pNaKtide was administered to a murine model of NASH: the C57Bl6 mouse fed a "western" diet containing high amounts of fat and fructose. The administration of pNaKtide reduced obesity as well as hepatic steatosis, inflammation and fibrosis. Of interest, we also noted …

2016 Soybean Insect Losses In The Southern Us, F. R. Musser, A. L. Catchot Jr., J. A. Davis, G. M. Lorenz, T. Reed, D. D. Reisig, S. D. Stewart, S. Taylor

Midsouth Entomologist

Estimated soybean insect costs and losses experienced during 2016 were collected and compiled following the 2016 growing season to provide a record of insect pressure and management practices for the year. This survey has been done annually in all participating states since at least 2011, with Mississippi having data back to 2004. The 2016 survey represents 10.8 million soybean acres across the southern United States. Overall, corn earworm was the most expensive pest in soybean followed closely by soybean looper and stink bugs. Total insect management costs rose slightly to $26.03 per acre during 2016. Estimated crop losses to insects …

A Role For Intercept Traps In The Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) Ipm Strategy At Ornamental Nurseries, C. T. Werle, B. J. Sampson, M. E. Reding

Midsouth Entomologist

Invasive ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) cause significant damage to ornamental nursery tree crops throughout the eastern United States. Depending on surrounding habitat, some nurseries can undergo large influxes of ambrosia beetles from the forest to susceptible nursery stock. Ethanol-baited intercept traps are highly effective as monitoring tools that can capture large numbers of dispersing ambrosia beetles. Beetle trap captures at varying distances within nursery interiors were determined across replicated transects that either included or lacked a row of edge intercept traps. Although nearly 90% of ambrosia beetle captures were from the edge intercept traps, there was no significant difference …

Preliminary Study Of Pan Trapping In Longleaf Pine Flatwoods In Central Florida, Usa, A. B. Orfinger, T. B. Cogen, G. R. Irons, K. V. Kabilan, B. Molligoda, A. L. Moody, J. F. Wisdo, K. F. Phillips

Midsouth Entomologist

It is well documented that pan trap color greatly influences arthropod catch composition. Additionally, it has been demonstrated that pan trapping catch varies with habitat. The goal of this study is to present preliminary data on pan trap yield from an imperiled ecosystem marked by high rates of endemicity, longleaf pine flatwoods. Yellow, blue, and white pan traps were evaluated in a section of longleaf pine flatwoods in June 2017 in central Florida, USA. A total of 360 arthropod specimens representing nine taxonomic orders were recovered, with 117 individuals from yellow traps, 125 from blue, and 118 from white. Contrary …

Diastolic Dysfunction In Coronary Artery Disease, Sarwat Hashmi, H R. Ahmad, Hasanat Sharif, Syedah Saira Bokhari

Department of Biological & Biomedical Sciences

No abstract provided.

Green Tea Proanthocyanidins Cause Impairment Of Hormone-Regulated Larval Development And Reproductive Fitness Via Repression Of Juvenile Hormone Acid Methyltransferase, Insulin-Like Peptide And Cytochrome P450 Genes In Anopheles Gambiae Sensu Stricto, Jackson M. Muema, Steven G. Nyanjom, James M. Mutunga, Sospeter N. Njeru, Joel L. Bargul

All Peer-Reviewed Publications

Successful optimization of plant-derived compounds into control of nuisance insects would benefit from scientifically validated targets. However, the close association between the genotypic responses and physiological toxicity effects mediated by these compounds remains underexplored. In this study, we evaluated the sublethal dose effects of proanthocyanidins (PAs) sourced from green tea (Camellia sinensis) on life history traits of Anopheles gambiae (sensu stricto) mosquitoes with an aim to unravel the probable molecular targets. Based on the induced phenotypic effects, genes selected for study targeted juvenile hormone (JH) biosynthesis, signal transduction, oxidative stress response and xenobiotic detoxification in addition to vitellogenesis in females. …

The Role Of Hyalomma Truncatum On The Dynamics Of Rift Valley Fever: Insights From A Mathematical Epidemic Model, Sansao A. Pedro, Shirley Abelman, Henri E.Z. Tonnang

All Peer-Reviewed Publications

To date, our knowledge of Rift Valley fever (RVF) disease spread and maintenance is still limited, as flooding, humid weather and presence of biting insects such as mosquitoes, have not completely explained RVF outbreaks. We propose a model that includes livestock, mosquitoes and ticks compartments structured according to their questing and feeding behaviour in order to study the possible role of ticks on the dynamics of RVF. To quantify disease transmission at the initial stage of the epidemic, we derive an explicit formula of the basic reproductive number, R0. Using the concept of Metzler matrix, we state necessary conditions for …

Smokejumper Obituary: Jacobsen, Gerald L. (Missoula 1963), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Grass Floras, Manuals, And Checklist, James P. Smith Jr

Botanical Studies

No abstract provided.

The Systematics Of Vascular Plants: A Bibliography, James P. Smith Jr

Botanical Studies

No abstract provided.

Cytomegalovirus Serostatus, Inflammation, And Antibody Response To Influenza Vaccination In Older Adults: The Moderating Effect Of Beta Blockade, Rebecca G. Reed, Richard N. Greenberg, Suzanne C. Segerstrom

Psychology Faculty Publications

Cytomegalovirus (CMV) has been implicated as a factor in immunosenescence, including poor antibody response to vaccination and higher immune activation and inflammation. Some people may be more or less vulnerable to the negative effects of CMV. The present investigation tested the effects of beta-blocker use and chronological age on the associations between CMV and immunity in adults aged 60–91 (N=98; 69% CMV seropositive) who were administered the trivalent influenza vaccine for up to 5 years. Peak antibody response, corrected for baseline, and spring (persistent) antibody response, corrected for peak, were assessed, as well as beta-2 microglobulin (β2μ) and interleukin-6 (IL-6). …

Assessing Anther Extrusion And Its Effect On Us Hard Winter Wheat (Triticum Aestivum L.) Hybrid Seed Production, Nicholas Garst

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The promise of higher grain yields as a result of the development and production of hybrid wheat (Triticum aestivum L.) has not been fully realized primarily due to the high cost of seed production. Anther extrusion is a key trait that improves pollen availability, and thus, is expected to enhance hybrid wheat seed production yields. Hard winter wheat germplasm adapted to the US Great Plains was visually assessed for anther extrusion in the field and greenhouse environments. Significant genotypic differences were detected and high broad-sense heritability was calculated (ranging from 0.62 to 0.85) for anther extrusion in the field. …

2016 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

Fourteen Nebraska counts were held during the 2016 CBC season, which is one less than last year. The Harrison and Crawford counts were cancelled this year, but in the opposite corner of the state, a new count was started that includes Indian Cave State Park. An unusually mild November and a seasonable start to December were followed by a modest cold snap during the second week of the month. The Harlan County count was run early in the period on the 15th with ample open water, and it produced a record number of species (90) for that circle. But in …

Brambling In Bellevue, Sarpy County, Loren Padelford, Babs Padelford

Nebraska Bird Review

As we were having breakfast on January 8, 2017, we spotted an unusuallooking bird with House Sparrows in the tree above our deck. After consulting the National Geographic field guide, we determined it was a Brambling. Soon it was feeding on the deck where we were able to get some photos. It was first spotted at 9:25 a.m. and again at 10:30. We put the information on NEBirds and by late morning birders began to arrive.The first group saw the bird at 12:02. By early afternoon we had standing room only in our family room. The bird appeared again at …

Foxo3 Increases Mir-34a To Cause Palmitate-Induced Cholangiocyte Lipoapoptosis, Sathish Kumar Natarajan, Bailey A. Stringham, Ashley M. Mohr, Cody J. Wehrkamp, Sizhao Lu, Mary Anne Phillippi, Duygu Dee Harrison-Findik, Justin L. Mott

School of Veterinary and Biomedical Sciences: Faculty Publications

Nonalcoholic steatohepatitis (NASH) patients have elevated plasma saturated free fatty acid levels. These toxic fatty acids can induce liver cell death and our recent results demonstrated that the biliary epithelium may be susceptible to lipotoxicity. Here, we explored the molecular mechanisms of cholangiocyte lipoapoptosis in cell culture and in an animal model of NASH. Treatment of cholangiocytes with palmitate (PA) showed increased caspase 3/7 activity and increased levels of cleaved poly (ADP-ribose) polymerase and cleaved caspase 3, demonstrating cholangiocyte lipoapoptosis. Interestingly, treatment with PA significantly increased the levels of microRNA miR-34a, a pro-apoptotic microRNA known to be elevated in NASH. …