Life Sciences Commons^™

Oxidative Stress-Driven Parvalbumin Interneuron Impairment As A Common Mechanism In Models Of Schizophrenia, P Steullet, J Cabungcal, J Coyle, M Didriksen, K Gill, Anthony-Samuel Lamantia, +Several Additional Authors

Pharmacology and Physiology Faculty Publications

Parvalbumin inhibitory interneurons (PVIs) are crucial for maintaining proper excitatory/inhibitory balance and high-frequency neuronal synchronization. Their activity supports critical developmental trajectories, sensory and cognitive processing, and social behavior. Despite heterogeneity in the etiology across schizophrenia and autism spectrum disorder, PVI circuits are altered in these psychiatric disorders. Identifying mechanism(s) underlying PVI deficits is essential to establish treatments targeting in particular cognition. On the basis of published and new data, we propose oxidative stress as a common pathological mechanism leading to PVI impairment in schizophrenia and some forms of autism. A series of animal models carrying genetic and/or environmental risks relevant …

Estimating Seasonal Variation In Condition Of Yellow Perch, Emily Anne Powers

Biology Graduate Theses

Research has shown that Yellow Perch Perca flavescens, found throughout lakes and streams of North America, are good indicators of environmental health. Due to the correlation between fish condition and ecosystem health it is essential to reliably estimate condition to better manage our fisheries. Therefore, the study objectives were to measure the seasonal changes in adult Yellow Perch percent dry weight (PDW), and compare the reliability of bioelectrical impedance analysis (BIA) and morphometric-based estimates of condition. The seasonal fluctuations in average monthly PDW were measured over a two- year period and were sinusoidal in shape with peaks in August 2015 …

The Experimental Flow To The Colorado River Delta: Effects On Carbon Mobilization In A Dry Watercourse, Thomas S. Bianchi, David Butman, Peter A. Raymond, Nicholas D. Ward, Rory J. Kates, Karl W. Flessa, Hector Zamora, Ana Arellano, Jorge Ramirez, Eliana Rodriguez

Marine Science Faculty Publications

Here we report on the effects of an experimental flood on the carbon cycling dynamics in the dry watercourse of the Colorado River in Mexico. We observed post-flood differences in the degree of decay, age, and concentration of dissolved organic carbon (DOC), as well as dissolved CH₄ and CO₂ concentrations throughout the study site. Our results indicate that this flooded waterway was a limited source of CH₄ and CO₂ to the atmosphere during the event and that DOC age increased with time of flooding. Based on our findings, we suggest that the interplay between storage and …

Challenges Of Conducting Animal Based Research And Teaching In Medical Colleges Of Karachi, Pakistan, Muhammad Ulusyar Khan, Faiq Amin, Fazal Manzoor Arain

Department of Biological & Biomedical Sciences

No abstract provided.

Development Of Ad Like Symptoms Following Co-Administration Of Alcl3 And D-Gal In Rats: A Neurochemical, Biochemical And Behavioural Study, Laraib Liaquat, Saara Muddasir, Sadia Adir, Zehra Batool, Saima Khaliq, Saiqa Tabassum, Shaista Emad, Syeda Madiha, Sidrah Shahzad, Saida Haider

Department of Biological & Biomedical Sciences

Alzheimer's disease (AD) is an age-related neurodegenerative disorder associated with neurochemical and neurobehavioural alterations. Aluminium (Al) is considered as a contributing factor in the etiology of several neurodegenerative disorders like AD. D-galactose (D-gal) is a physiological nutrient but over supply induces some neurochemical and biochemical changes that exacerbate natural aging process. In this study, we aimed to develop AD animal model by co-administration of Al and D-gal in rats. Male albino Wistar rats were intraperitoneally injected with AlCl₃ and D-gal at a dose of 150mg/kg and 300mg/kg respectively for one week. After one week rats were subjected to behavioural …

Site-Specific Variation In Gene Expression From Symbiodinium Spp. Associated With Offshore And Inshore Porites Astreoides In The Lower Florida Keys Is Lost With Bleaching And Disease Stress, Brianna Salas, Joshua Haslun, Kevin Strychar, Peggy Ostrom, James Cervino

Open Access Publishing Support Funded Articles

Scleractinian coral are experiencing unprecedented rates of mortality due to increases in sea surface temperatures in response to global climate change. Some coral species however, survive high temperature events due to a reduced susceptibility to bleaching. We investigated the relationship between bleaching susceptibility and expression of five metabolically related genes of Symbiodinium spp. from the coral Porites astreoides originating from an inshore and offshore reef in the Florida Keys. The acclimatization potential of Symbiodinium spp. to changing temperature regimes was also measured via a two-year reciprocal transplant between the sites. Offshore coral fragments displayed significantly higher expression in Symbiodiniumspp. …

Plasma Metabolites Reveal Changes In Physiological State Of Eared Grebes (Podiceps Nigricollis) During Three Non-Migratory Periods, Allison A. Yoshida

Theses

Energy storage and use during the annual cycle of migratory birds is dynamic especially during migration. However, considerable amounts of the annual cycle are spent not migrating, and the physiological state of birds during these non-migratory periods is not well understood. We compared plasma metabolites (TRIG, BUTY, URIC, GLY) in Eared Grebes (Podiceps nigricollis) residing at three important areas when they are not migrating: fall staging (postbreeding at Mono Lake, California), spring staging (pre-breeding at Great Salt Lake, Utah), and an extended stopover prior to spring staging (Salton Sea, California). Plasma metabolites were also measured in resting and active grebes …

Using A Respiratory Navigator Significantly Reduces Variability When Quantifying Left Ventricular Torsion With Cardiovascular Magnetic Resonance, Sean M. Hamlet, Christopher M. Haggerty, Jonathan D. Suever, Gregory J. Wehner, Kristin N. Andres, David K. Powell, Richard J. Charnigo, Brandon K. Fornwalt

Electrical and Computer Engineering Faculty Publications

Background: Left ventricular (LV) torsion is an important indicator of cardiac function that is limited by high inter-test variability (50% of the mean value). We hypothesized that this high inter-test variability is partly due to inconsistent breath-hold positions during serial image acquisitions, which could be significantly improved by using a respiratory navigator for cardiovascular magnetic resonance (CMR) based quantification of LV torsion.

Methods: We assessed respiratory-related variability in measured LV torsion with two distinct experimental protocols. First, 17 volunteers were recruited for CMR with cine displacement encoding with stimulated echoes (DENSE) in which a respiratory navigator was used to measure …

Pneb193-Derived Suicide Plasmids For Gene Deletion And Protein Expression In The Methane-Producing Archaeon, Methanosarcina Acetivorans, Mitchell T. Shea, Mary E. Walter, Nikolas Duszenko, Anne-Lise Ducluzeau, Jared Aldridge, Shannon K. King, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Gene deletion and protein expression are cornerstone procedures for studying metabolism in any organism, including methane-producing archaea (methanogens). Methanogens produce coenzymes and cofactors not found in most bacteria, therefore it is sometimes necessary to express and purify methanogen proteins from the natural host. Protein expression in the native organism is also useful when studying post-translational modifications and their effect on gene expression or enzyme activity. We have created several new suicide plasmids to complement existing genetic tools for use in the methanogen, Methanosarcina acetivorans. The new plasmids are derived from the commercially available E. coli plasmid, pNEB193, and cannot replicate …

Microbial Strains And Methods Of Making And Using, Nicole Roswitha Buan Murphy, Jennifer Catlett

School of Biological Sciences: Faculty Publications

Microbial strains are provided, as are methods of making and using such microbial strains.

Methane is an increasingly important global energy Source, both as the primary component of natural gas and as a biologically produced fuel. Currently, Sweden uses 100% renewable methane (biogas) in place of compressed natural gas (CNG) for transportation fuel, and several European countries are implementing policies to increase their pro portion of biogas transportation fuel. In addition, methane is a valuable chemical for conversion into other higher-order carbon compounds (e.g., methanol, formate, alkanes, ter penes). More than 50% of the world's methane is produced biologically by …

Blue-Light Induced Accumulation Of Reactive Oxygen Species Is A Consequence Of The Drosophila Cryptochrome Photocycle, Louis David Arthaut, Margaret Ahmad, Nathalie Jourdan, Ali Mteyrek, Maria Procopio, Mohamed A. El-Esawi, Alain D'Harlingue, Pierre Etienne Bouchet, Jacques Witczak, Carlos F. Martino

Biomedical Engineering and Sciences Faculty Publications

Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD∗-) …

An Open-Sourced Statistical Application For Identifying Complex Toxicological Interactions Of Environmental Pollutants, Jordan T. Perkins, Michael C. Petriello, Li Xu, Arnold J. Stromberg, Bernhard Hennig

Superfund Research Center Faculty Publications

The rising number of chemicals that humans are exposed to on a daily basis, as well as advances in biomonitoring and detection technologies have highlighted the diversity of individual exposure profiles (complex body burdens). To address this, the toxicological sciences have begun to shift away from examining toxic agents or stressors individually to focusing on more complex models with multiple agents or stressors present. Literature on interactions between chemicals is fairly limited in comparison with dose-response studies on individual toxicants, which is largely due to experimental and statistical challenges. Experimental designs capable of identifying these complex interactions are often avoided …

Impact Of Nutrition On Pollutant Toxicity: An Update With New Insights Into Epigenetic Regulation, Jessie B. Hoffman, Michael C. Petriello, Bernhard Hennig

Superfund Research Center Faculty Publications

Exposure to environmental pollutants is a global health problem and is associated with the development of many chronic diseases, including cardiovascular disease, diabetes and metabolic syndrome. There is a growing body of evidence that nutrition can both positively and negatively modulate the toxic effects of pollutant exposure. Diets high in proinflammatory fats, such as linoleic acid, can exacerbate pollutant toxicity, whereas diets rich in bioactive and anti-inflammatory food components, including omega-3 fatty acids and polyphenols, can attenuate toxicant-associated inflammation. Previously, researchers have elucidated direct mechanisms of nutritional modulation, including alteration of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling, …

Students' Perceptions Of Usefulness Of Anatomy Demonstrations In Traditional And Hybrid Undergraduate Medical Education Curricula, Ayesha Saba Naz, Rehana Rehman, Zehra Jamil, Khalid Ahmed, Ambreen Surti

Department of Biological & Biomedical Sciences

A cross-sectional study was carried out to study students' perceptions on the usefulness of Anatomy demonstrations (AD) in the undergraduate medical education by comparing the Conventional Medical College (CMC) and problem-based learning as hybrid curriculum (HMC). Purposive sampling technique was used and all students were included. The completed questionnaire responses were returned by 92 CMC and 87 HMC students. CMC cohort understood the structural relationship more than HMC (p=0.03). AD helped 50 students (54%) of CMC to get through the theory examination, however 73 (84%) students of HMC found them useful in preparation for theory examinations (p<0.001). The importance of AD as a major content delivery strategy cannot be overemphasized in the anatomy curriculum and useful teaching strategies from various undergraduate medical curricula, such as the use of the plastic and plastinated models and the session handouts.

Soluble Transferrin Receptor, Ferritin Index In Pakistani Population, Faiza Alam, Nabeel Ashraf, Ramsha Kashif, Hashaam Arshad, Syeda Sadia Fatima

Department of Biological & Biomedical Sciences

Inflammation affects the reliability of ferritin. The serum level of transferrin receptor protein (sTfR) represents true demand of iron in the body. This study attempts to identify levels of sTfR and correlate the trends of sTfR/ferritin index with BMI in the population of Karachi. 132 gender matched volunteers between the ages of 20-60 years were recruited for this cross-sectional study. BMI was calculated using the formula: (weight in kg / height in m2). Following groups were made according to South Asian criteria of BMI; Group A: normal weight (18.0-22.9 kg/m2), Group B: overweight (23.0-24.9 kg/m2), Group C: obese ( > 25.0 …

Annual Color Photo Section 2017, The Nebraska Bird Review

Nebraska Bird Review

For our color photo section this year we asked especially for photos of birds in unexpected places. The images on this page and the following two pages show birds in surprising locations or unusual geographic ranges.

32 images

Canyon Wren in wheel of the photographer’s Subaru at Mary Sue Shoemaker’s ranch in central Cherry Co, 20 Nov 2016. 5th record of this species for Nebraska. Photo by Joel Jorgensen.

Great Horned Owl at water feature in the photographer’s yard, Dixon Co., 18 April 2017. Photo by Jan Johnson.

Brambling, 4th record in Nebraska, coming to the feeders at the home …

Winter Field Report, December 2016 To February 2017, W. Ross Silcock

Nebraska Bird Review

This was an interesting winter from several standpoints. The trend of unexpected winter occurrences and earlier arrivals in “spring” continued. Mid-winter records of several species were notable: Rock Wren, Marsh Wren, American Pipit, Pine Warbler, and Chipping Sparrow. Rarities (see below) that could be considered in this category as well were Golden-crowned Sparrow and Lesser Goldfinch. For earlier arrivals, there was a noticeable influx 14-18 Feb of various species that provided rather early dates for those species. In this group were Blue-winged Teal, Ruddy Duck, Double-crested Cormorant, American White Pelican, and Turkey Vulture.

Some amazing numbers were reported, including a …

The Nebraska Bird Review, Volume 85 March 2017 Number 1

Nebraska Bird Review

Winter Field Report, Dec. 2016–Feb. 2017, by W. Ross Silcock … 2–22 & 31

Annual Color Photo Section … 23–30

Brambling in Bellevue, Sarpy County by Loren and Babs Padelford … 32–33

2016 Christmas Bird Counts, by Don Paseka … 34–49

NOU Annual Treasurer's Report for 2016, by Jan Johnson … 50

Subscription and Organization Information … 51

Textural, Color And Sensory Attributes Of Fruits And Vegetables Dried Using Electric Forced-Air And Solar Dehydrators, Edward Wayne Crossen

Theses and Dissertations

Direct sun drying is one of the oldest methods of food preservation. A drying method that utilizes solar energy while minimizing negative aspects of sun drying is a solar dryer. However, research about the quality characteristics of fruits and vegetables dried using a solar dryer compared to a traditional dryer is lacking. To measure this, apple, tomato and carrot slices, and grape halves and whole peas were prepared and dried using either a solar or FA dehydrator (FA), Consumer liking was measured using two sensory panels that asked panelists 9-point hedonic questions between like products. Quality differences were analyzed using …

Forage News [2017-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Alfalfa and Stored Forages Conference Award Winners
• Farmer Success with Novel Endophyte Fescue
• Making High Quality Baleage
• Don't Leave the Leaves
• Fertilizing Cool Season Pastures

Smug2 Dna Glycosylase From Pedobacter Heparinus As A New Subfamily In Udg Superfamily, Panjiao Pang, Ye Yang, Jing Li, Zhong Wang, Weiguo Cao, Wei Xie

Publications

Base deamination is a common type of DNA damage that occurs in all organisms. DNA repair mechanisms are essential to maintain genome integrity, in which the base excision repair (BER) pathway plays a major role in the removal of base damage. In the BER pathway, the uracil DNA glycosylase superfamily is responsible for excising the deaminated bases from DNA and generates apurinic/apyrimidinic (AP) sites. Using bioinformatics tools, we identified a family 3 SMUG1-like DNA glycoyslase from Pedobacter heparinus (named Phe SMUG2), which displays catalytic activities towards DNA containing uracil or hypoxanthine/xanthine. Phylogenetic analyses show that SMUG2 enzymes are closely related …

First Record Of Nestling Relocation By Adult Gyrfalcons (Falco Rusticolus) Following Nest Collapse, Bryce W. Robinson, Neil Paprocki, David L. Anderson, Marc J. Bechard

Raptor Research Center Publications and Presentations

Nest collapse has been documented in many bird species, with little discussion of adult behavior following collapse. We present evidence of a partial collapse of a Gyrfalcon (Falco rusticolus) nest during the nestling period and the subsequent adult response. A nest camera captured the nest collapse and showed one adult Gyrfalcon holding a live nestling in its beak before leaving the nest. Later, we found the surviving nestling alive in an alternate nest 5 m from the original nest, presumably transported there by the adult. We believe this is the first report of an adult Gyrfalcon moving a …

Homestead National Monument Of America, Bat Acoustic Monitoring, September 2016, Daniel S. Licht

United States National Park Service: Publications

Abstract

Homestead National Monument of America is a 211-acre park located in an agrarian landscape in southeastern Nebraska. From September 16 to October 1, 2016, park staff deployed acoustic monitors at three sites in the park for purposes of monitoring night-time bat activity. The three sites averaged 179, 48, and 33 bat detections per night. Night-time bat activity was generally highest in the 1-2 hours following sunset.

Based on the acoustic surveys the big brown (Eptesicus fuscus), eastern red (Lasiurus borealis), northern long-eared (Myotis septentrionalis) and evening bats (Nycticeius humeralis) were present at the …

Mechanisms Of Thymic Toxicity Induced By Environmentally Relevant Concentrations Of Arsenic, Huan Xu

Biomedical Sciences ETDs

Arsenic, a ubiquitous environmental contaminant, is known to cause immunotoxicity. However, the mechanisms of arsenic induced immunotoxicity are still not clear. Arsenite (As⁺³), the trivalent inorganic form of arsenic, was found to increase DNA damage and inhibit PARP activity in thymus cells at 50 nM in vitro. Oxidative stress and double strand breaks (DSBs) formation occurred in thymus cells exposed to As⁺³ at 500 nM. The genotoxicity was found to be related to As⁺³ exporter expressions and reversed by zinc supplement. IL-7 signaling, the critical cytokine signal for the early DN thymic T cell proliferation, …

The Insulin-Like Growth Factor-1 (Igf1) System As A Potential Biomarker For Nutritional Status And Growth Rate In Pacific Rockfish (Sebastes Spp.), Nicole L. Hack

Master's Theses

Growth performance in vertebrates is regulated by environmental factors including the quality and quantity of food, which influences growth via endocrine pathways such as the growth hormone (GH) / insulin-like growth factor somatotropic axis. In several teleost fishes, circulating concentrations of insulin-like growth factor-1 (Igf1) correlate positively with growth rate, and it has been proposed that plasma Igf1 levels may serve as an indicator of growth variation for fisheries and aquaculture applications. Here, I tested whether plasma Igf1 concentrations might serve as an indicator of somatic growth in olive rockfish (Sebastes serranoides), one species among dozens of rockfishes …

Characterization Of Pit Latrines To Support The Design And Selection Of Emptying Tools In Peri-Urban Mzuzu, Malawi., Rashid Chiposa, Rochelle H. Holm, Chimuleke Munthali, Russel C.G. Chidya, Francis L. De Los Reyes

Faculty and Staff Scholarship

The urban areas of many low-income countries must balance a rising demand for pit latrines for household sanitation provision against limitations in space, resulting in a need for pit latrine emptying services. This study was undertaken in the peri-urban neighborhood of Area 1B in the city of Mzuzu, Malawi, to examine the characteristics of household pit latrines for designing and selecting pit latrine emptying tools. We used 150 structured household surveys and field observations. From this, a subset was selected and 30 manual cone penetrometer tests were conducted at full latrines. Chemical oxygen demand analysis was also performed for 14 …

Ecological Correlations Of Dietary Food Intake And Mental Health Disorders, Jordan Hoerr, Joshua Fogel, Benjamin Van Voorhees

Publications and Research

This paper examines the ecological association of dietary food intake with mental health outcomes on the group level across countries. Published data from the World Mental Health Survey were used to compare lifetime prevalence of four categories of mental health disorders (anxiety disorders, mood disorders, impulse control disorders, and substance use disorders) with a country’s fish/seafood and sugar/sweetener supply quantity using the Spearman rank correlation. Data were compared for 17 countries across the world. Sugar and sweetener supply quantity was significantly and positively associated with anxiety disorders (rho = 0.75, p = 0.001), mood disorders (rho = 0.75, p = …

A Pilot And Feasibility Study To Assess Children’S Consumption In Quick-Service Restaurants Using Plate Waste Methodology, Juliana F.W. Cohen, Susan B. Roberts, Stephanie Anzman-Frasca, Madeleine Mg. Gamache, Vanessa M. Lynskey, Emilia Matthews, Megan P. Mueller, Shanti Sharma, Christina D. Economos

Health Sciences Faculty Publications

Background: Children regularly consume foods from quick-service restaurants (QSR), but little is known about the foods that children order, the calories and nutrients consumed, the accuracy of stated calorie information, or the ability to assess food orders and consumption in QSRs. This study evaluated the feasibility of plate waste collection in QSRs and examined children’s orders and consumption of meals from the standard and children’s menus. Additional aims were to examine if the meals ordered met healthier standards for children’s menu items and determine the accuracy of the QSR-stated energy content of foods. Methods: Fifteen QSRs, two malls, and 116 …

Patellar Tendon Straps Decrease Pre-Landing Quadriceps Activation In Males With Patellar Tendinopathy, Adam B. Rosen, Jupil Ko, Kathy J. Simpson, Cathleen N. Brown

Health and Kinesiology Faculty Publications

Objective: To determine if patellar tendon straps altered quadriceps’ muscle activity during a drop-jump landing in males with and without patellar tendinopathy.

Design: Case-control.

Settings: Biomechanics Research Laboratory.

Participants: Twenty recreationally-active males participated: ten (age=21.3±2.4 years, height=182.8±5.3cm, mass=81.7±8.6kg) with patellar tendinopathy; ten (age=22.0±1.6 years, height=185.7±4.5 cm, mass=82.2±9.8kg) were healthy with no history of tendinopathy.

Main Outcome Measures: Electromyography (EMG) data for the vastus medialis (VM), rectus femoris (RF), and vastus lateralis (VL) muscles were collected. Five 2-legged 40cm drop-jumps were performed wearing a patellar tendon strap and 5 with no-strap in a counterbalanced order.

Root-mean …

The North American Perching And Dabbling Ducks: Their Biology And Behavior, Paul Johnsgard

Zea E-Books Collection

This volume, the fourth in a series of books that collectively update and expand P. A. Johnsgard’s 1975 The Waterfowl of North America, summarizes research findings on this economically and ecologically important group of waterfowl. The volume includes the mostly tropical perching duck tribe Cairinini, of which two species, the muscovy duck and the wood duck, are representatives. Both species are adapted for foraging on the water surface, mostly on plant materials, but typically perch in trees and nest in elevated tree cavities or other elevated recesses. This volume also includes the dabbling, or surface-feeding, duck tribe Anatini, a …