Life Sciences Commons^™

An Investigation Into The Correlation Between Step Width Variability And Balance Deficits In Patients With Chronic Obstructive Pulmonary Disease, Jordan Freeman

UNO Student Research and Creative Activity Fair

Patients with chronic obstructive pulmonary disease (COPD) experience a higher incidence of falls. Further, gait variability has been shown to be a useful predictor of future falls. It is unknown if balance measures and gait variability are related. Our purpose was to investigate if step width (SW) variability is related to balance deficits in patients with COPD. We hypothesized that functional balance tests will closely correlate to the step width variability as compared to subjective balance questionnaires. Nine subjects with COPD and six controls performed a series of balance measures: sensory organization test (SOT), motor control test (MCT), Fullerton advanced …

The Effect Of Bilateral Vestibular Stimulation On Locomotor Adaptation, Allison Hoover

UNO Student Research and Creative Activity Fair

Locomotor adaptation requires input from vision, somatosensory and vestibular systems. The contribution of the vestibular system is important for maintaining postural control during gait specifically orientation of the head on the trunk and acceleration (linear and angular) feedback. Disrupting this system causes instabilities during locomotion. However, it is not clear, how much the vestibular system contributes to gait adaptation. Eighteen healthy adults adapted to a split-belt treadmill task with or without additional vestibular perturbation using bilateral mastoid vibration. The symmetry index for step length and step time, as well as the change in standard deviation in stride time for both …

Functional Genetics Of Mind-Meld In Drosophila Melanogaster, Candi R. Senior-Remsa

UNO Student Research and Creative Activity Fair

Immunohistochemistry (IHC) is a useful research tool used to localize specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions. To obtain a clearer understanding of the cellular and subcellular localization of the Mind-Meld (MMD) protein during the developing Drosophila melanogaster embryo double indirect immunofluorescence was used to colocalize MMD with other proteins found in the fruit fly. An affinity-purified antibody raised in rabbit identifying all MMD isoforms was used with the fluorophore-labeled secondary antibody Alexa Fluor 594 specific to rabbit IgG. Well-characterized murine monoclonal antibodies with known subcellular localization and function in Drosophila: Fascicilin II (FASII), Short …

Assessment Of Olfactory-Based Social Recognition: Designing A Paradigm For Marmosets, Stephanie Womack

UNO Student Research and Creative Activity Fair

Navigation of the social world depends largely on one’s responsiveness to social stimuli and information from a wide range of senses can be used to discriminate between individuals. Mammals use several sensory modes to communicate and respond to their surroundings, but olfaction is the dominant sense across most species. Chemosignals provide a wealth of information including sex, age, reproductive status, and individual identity. Currently, assessment of olfaction is largely limited to the habituation-dishabituation paradigm or the two-choice discrimination task used primarily in rodents. These paradigms are limited by their reliance on inherent reward of interaction with stimuli without providing additional …

Augmenting Human Muscle Performance Through Added Foot Stiffness, Samuel F. Ray

UNO Student Research and Creative Activity Fair

In human walking, the relationship between foot and ankle structure and function is not fully known. The foot and toe structures seem to dissipate energy while the ankle generates force through the plantarflexor muscles. Increasing foot stiffness through added carbon fiber insoles has been shown to increase force output and decrease contraction velocity of the ankle plantarflexor muscles. This shift in the muscular force-velocity operating range may be beneficial in fast walking. During fast walking, muscle fascicles contract at high velocities and low force output. We predict that added foot stiffness can reduce the metabolic cost of fast walking by …

Taxonomic Placement Of The Nidulariaceae Of Nebraska And Iowa Based On Molecular And Morphological Data, Goodmond H. Danielsen Iv

UNO Student Research and Creative Activity Fair

Proper taxonomic and molecular study is necessary to better organize closely related species that may not only be used for human needs, but also in the quest for knowledge of how the world around us works. Though important, the taxonomic placement of Nebraskan and Iowan bird’s nest fungi (Nidulariaceae) is not well documented. Here, phylogenetic analysis is used to place the Nidulariaceae of Nebraska and Iowa in the tree of life using molecular and morphological techniques. Based on these results and results of further work done on these specimens, it will be possible to better place the Nidulariaceae of Nebraska …

Gait Biomechanics In Patients With Peripheral Artery Disease After Revascularization, Sarah Baker

UNO Student Research and Creative Activity Fair

Peripheral artery disease (PAD) is a cardiovascular disease manifesting blockages to arteries while limiting blood flow to the legs. Patients with PAD have pain/tingling in calves, thighs, and/or buttocks emanating by physical activity called intermittent claudication. PAD causes physical function limitations and increases cardiovascular morbidity by 3-6 times. PAD becomes particularly more prevalent with age. Patients with PAD walk differently than their healthy counterparts. Surgical revascularization to restore blood flow is the typical intervention, but whether walking patterns are restored following surgery has not been studied. This study compared walking patterns of patients with PAD at baseline and six-months post-revascularization. …

A Comparison Of Balance Between A Smoker And Never-Smoker, Robert J. Barber

UNO Student Research and Creative Activity Fair

“Chronic obstructive pulmonary disease (COPD) the third leading cause of death in the United States.” “While COPD is defined in terms of fixed airflow limitation, it’s also characterized by the frequent association of complications outside the lung, including compromised balance.” Research has shown “medio-lateral (i.e., side-to-side) balance is altered in patients with COPD compared to controls and is further affected by challenging sensory systems (e.g., eyes closed or uneven surface).” “Smoking increases risk for onset of COPD”, therefore the objective of this study was to investigate balance in smokers. It’s hypothesized that smokers will demonstrate balance deficits, compared to never-smokers. …

Species Diversity And Relative Abundance Of Reptiles And Amphibians At Glacier Creek Preserve, Cori L. Borgheiinck

UNO Student Research and Creative Activity Fair

Glacier Creek Preserve, located in Bennington, Nebraska, is dedicated to restoring land to native prairie. Restoration at Glacier Creek Preserve began in 1970. Over the years, the preserve has acquired more land, as well as acquiring more habitat diversity. This research is the first to look at the community dynamics of herpetofauna in the preserve. The sampling areas were determined by choosing seven random sites to represent the following habitats, (1) prairie, (2) non-prairie, (3) prairie burned in 2014, and (4) prairie burned in 2016.

Data sampling occurred from April 2016 to October 2016 and from February 2017 to March …

Effects Of Hypobaric And Normobaric Hypoxia On Heart Rate And Blood Oxygen Saturation, Madison Brooks Coleman

UNO Student Research and Creative Activity Fair

There has been recent debate on the difference in physiological response between exposure to normobaric hypoxia (oxygen fraction altered) and hypobaric hypoxia (barometric pressure altered). Purpose: To determine the effects of normobaric normoxia (NN), hypobaric hypoxia (HH), and normobaric hypoxia (NH) on heart rate and arterial oxygen saturation (SaO₂) after exercise. Methods: Fifteen recreationally active participants (eight males and seven females) completed three separate 1 h cycling bouts at 70% of their peak aerobic capacity. Following each trial, participants recovered in the supine position for 4 h in NN (975 m), HH (4420 m), or NH (4420 m). …

The Effect Of Observing Aggressive Interactions On The Personality Traits Of Danio Rerio, Jessica L. Bargstadt

UNO Student Research and Creative Activity Fair

Social animals are easily influenced by other members of their species. Individuals will adjust their behaviors and actions both when observing another conspecific and when interacting with one. The effect that these social experiences can have on an individual vary in both magnitude and permanence, depending on the type of experience. We seek to examine the lasting effect that observing an aggressive interaction can have on the personality type of an individual.

Personality traits of Danio rerio will be examined in a novel environment setting in which the subject’s movements are recorded to determine their reactivity or proactivity in response …

Seroprevalence Of Babesia Microti In Patients With Lyme Disease, Sabino Curcio

Discovery Day (formerly Post & Beyond)

Babesiosis is a tickborne disease caused by Babesia microti, an intracellular parasite of red blood cells. Presently, B. microti is the most common and deadly pathogen transmitted by blood transfusion. B. microti infects humans via Ixodes scapularis ticks, the same vector carrying Borrelia burgdorferi, the causative agent of Lyme disease. Co-infection with these two diseases increases the severity of symptoms, posing a major therapeutic challenge. We aimed to determine the co-infection rate of B. microti in individuals who tested positive for Lyme disease. Sera obtained from 154 individuals in New York State were collected and tested by immunofluorescence assay. Of …

Recruitment, Survival, And Parasitism Of Monarchs In Residential Gardens And Conservation Areas, Emily A. Geest

UNO Student Research and Creative Activity Fair

Monarch butterflies (Danaus plexippus) are suffering from declining populations due to habitat loss. To help offset this habitat loss conservationists have encouraged planting milkweed gardens to increase the availability of host plants for larvae. A tachinid fly (Lespesia archippivora) that causes mortality parasitizes monarchs. Understanding the influence these gardens can have on parasitism rates and monarch recruitment is vital to evaluating their effectiveness. I am quantifying egg/larval abundance and demography at conservation areas and residential gardens. Additionally, I am collecting and rearing monarch larvae to compare parasitism rates in both areas. My preliminary results suggest that …

Docking Studies Of Isoform-Selectivity Of Phosphatidylinositol 3-Kinase (Pi3k) Inhibitors, Kaitlin Goettsch

UNO Student Research and Creative Activity Fair

Phosphatidylinositol 3-kinases (PI3Ks) and their related pathways are reputed targets for drug-based anticancer therapies. Mutations in PI3K genes, expression, and pathways are frequent among multiple cancer types. Four isoforms of PI3Ks exist: α, β, γ, & δ and studies have identified several ligands for each isoform which are capable of serving as inhibitory therapeutic compounds. However, the biochemical efficacy of these molecules varies and the isoform selectivity is not well understood. In this study, we applied in silico docking methods and free energy calculation methods to estimate the binding of reported PI3K ligands against 5 PI3K structures: PI3Kα (PBD ID: …

Influence Of Neuromuscular Fatigue On The Reliability Of Gait Variability Measures, Nicholas Reynolds

UNO Student Research and Creative Activity Fair

Walking in healthy young adults displays an optimal pattern of variability from one stride to the next. This level ensures that each step taken is not stereotyped but also not completely unpredictable. Previous studies have investigated differences in stride-to-stride characteristics comparing groups of young to groups of elderly. The consistency of gait variability measures on an individual level remains to be determined. The first aim of this study is to determine the between day and between trial consistency of gait variability measures in healthy young adults. We hypothesize that there will be a high level of consistency from day-to-day and …

Effects Of Serial Subtractions On Elderly Gait Speed In A Virtual Reality Setting, Taylor Leeder

UNO Student Research and Creative Activity Fair

Falls are the leading cause of fatal injuries in older adults aged 65+ with one in three adults falling each year. Cognitive processes are involved in gait, and thus, as cognitive changes are normative with age, this may impact fall risk in the elderly. Sixteen older adults aged 70.8±5.3 years took part in a dual-task (DT) study where they walked on a split-belt self-paced treadmill with and without a virtual reality (VR) setting while simultaneously performing a serial subtraction (SS) task. For SS, individuals were given a random 3-digit starting number and told to subtract by three from that number …

Identification Of Optimal Parameter Ranges In Building And Assessing Correlation Networks Built From Gene Expression., Qianran Li

UNO Student Research and Creative Activity Fair

In this project, I investigate and define the range of acceptable outputs of a gene expression correlation network model. Gene expression refers to the amount of product made by a gene under a given biological condition. A correlation network is a graphical model where the nodes represent genes in an organism and the edges represent the amount of correlation between genes, based on their expression. Correlation network modeling has been used in cellular and biomedical domains to identify functional relationships between genes. The network model in general is a wonderful tool for showcasing relationships, but often times they are misused …

Human Stress Protein Response To Exercise And The Environment, Rebecca Cuthbert

UNO Student Research and Creative Activity Fair

Stress proteins are molecular chaperones that protect cells from the damaging effects that can be caused by exercise and environmental changes. Cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) protect cells from mild hypothermia and hypoxia. They allow cells to rapidly respond to environmental stresses by regulating gene expression. Heat shock proteins 27, 70, and 90 protect skeletal tissue from exercise and heat stress. They accelerate cellular repair and prevent misfolding of proteins. These proteins, especially CIRP and RBM3, have been studied in cell cultures and animal models, but limited data exist in humans. Purpose: To …

Development Of Molecular Tools For Identification Of Prairie Terrestrial And Wetland Algae, Esther Mundembo Kapinga

UNO Student Research and Creative Activity Fair

Algae are a diverse group of photosynthetic protists. Green algae are typically unicellular, though some may occur as multicellular colonies, and they are found across a wide range of habitats, including marine, freshwater, and terrestrial environments. As photosynthetic producers and sometimes symbionts, algae can occupy important roles in an ecosystem; in other circumstances they can have negative impacts on environmental quality. Estimations of the number of green algal species range from eight thousand to over fifteen thousand. The identification of individual algal species can be problematic due to their small size and typically simple cell anatomy. For this reason, molecular …

Oxygen Transport In Normobaric Versus Hypobaric Hypoxia, Christina N. Angeli

UNO Student Research and Creative Activity Fair

BACKGROUND: Various physiological changes occur in the human body when ascending to altitude. Exposure to hypoxia increases heart rate and decreases arterial oxygen saturation (SaO₂), with both changes being expedited during exercise. Incremental exercise in ambient conditions decreases muscle oxygenation, specifically with increasing intensity. Previous research has examined heart rate and SaO₂ in response to normobaric versus hypobaric hypoxia. However, there is lack of research examining muscle oxygenation in response to these conditions. Therefore, additional research investigating acute responses to normobaric and hypobaric hypoxia during exercise is warranted. PURPOSE: To examine acute responses in arterial and muscle …

Landscape Features That Predict The Occurrence Of Shorebirds In Agriculture Fields During Migration In The Rainwater Basin, Nebraska, Lindsay Ann Brown

UNO Student Research and Creative Activity Fair

Some upland shorebird species are undergoing population declines. During their northward migration, these species primarily stop and use agricultural landscapes. This behavior is concerning because these landscapes are typically lower in resources, which could compromise energy demands during migration. There is a lack of information on what landscape features influence where species stop and on how the species use agricultural habitats. To close the gap, I compared landscape features between site where species-present and where were species-absent sites and developed models to predict migratory stopping sites. I focused on three uplands species: American Golden-Plovers, Buff-breasted Sandpipers, and Upland Sandpipers. Each …

A Network Mdoel To Investigate Robustness Of Gene Expressions, Naresh Pasupuleti

UNO Student Research and Creative Activity Fair

Correlation networks are ideal to describe the relationship between the expression profiles of genes. Gene expression is a characteristic exhibited by a particular gene. Our body has thousands of genes; each of them expresses differently, and each one of them has a particular function associated with them. When genes corresponding to a particular part of the body becomes non-functional, i.e., not expressed, then the function corresponding to that part of the body does not happen, thereby causing impairment or mutations. Co-regulation is a method involved in clustering analysis to find genes that perform similar functions. We want to identify genes …

Treadmill Walking In Claudication, Shane Lentz

UNO Student Research and Creative Activity Fair

INTRO. Claudication is lower leg pain that resides with rest. There are two potential causes of claudication: Peripheral Arterial Disease (PAD) and Lumbar Spinal Stenosis (LSS). Vasculogenic claudication is the defining characteristic of PAD. There is little research that exists to differentiate the two forms of claudication and there is little no non-invasive to determine which condition is causing the claudication. The purpose of this study is to utilize biomechanics to distinguish between individuals with claudication that arises from neurogenic versus vasculogenic causes. METHODS. Four LSS subjects completed a walking test on a treadmill (Gardner Test). The treadmill is pressure …

Identification Of Genes Involved In Diauxic Shift Of Saccharomyces Cerevisiae Through Gateway Node Analysis., Emily Pachunka

UNO Student Research and Creative Activity Fair

The use of high-throughput assays, or experiments yielding large data sets, in biological research has become a standard practice in laboratories throughout the world. Because such investigations have the ability to produce high volume and comprehensive data sets, it is then important to develop methods that allow researchers to quickly pull meaningful information from an overwhelming amount of data. Network modeling has become a popular technique for visualizing and analyzing large biological data sets. A network is a basic graph with nodes and edges (i.e. social networks) that also integrates complex principles of graph theory for deeper analysis and pattern …

Session B-1: Current Events In Biology, Crystal Randall, Don Dosch, Sarah O'Leary-Driscoll

Professional Learning Day

Teaching fundamentals, while important, can sometimes take a toll on our motivation and enthusiasm for our subject because there are rarely significant changes in the content we might present from year to year. However, keeping up with the current progress of our field, what’s new and exciting in research, and how current advancements can impact our health, well-being, and society in the future can keep us inspired, and help us continue to inspire our students. Come discuss what’s happening in biology news today, hear what we’re excited about and bring your own ideas to share!

Correlation Networks: Causative Relationships From Gene Expression Data, Grogan W. Huff

UNO Student Research and Creative Activity Fair

Genes that share expression conditions show a biological correlation, and no modern method of visualization displays these intricate co-expression patterns better than a graph. Structural observations about a co-expression graph can reveal the secrets of the biological system that it models, but experimentally validated co-expression graphs are pain-staking work to produce. Present day correlation network analysis shows potential for drawing conclusions from large volumes of biological systems data in an inexpensive and easy-to-produce way, however, work remains to confirm the appropriateness and scope of such methods for specific, scientific application. Toward this effort, we generated a Pearson correlation network from …

Session C-1: Modeling Stem Activities Into Classroom Practice, Sowmya Anjur

Professional Learning Day

Students understand concepts better when they have had a chance to work hands on with relevant material. Examples will be presented from my classroom where difficult concepts have been modeled into simple experiments with considerable success in enhancing student understanding. Special focus will be given to selected topics that students seem to have the most difficulty grasping. The objective is to enable students to transfer their understanding to solve complex problems with considerable ease and apply their understanding to real world scenarios on assessments. Suggestions will also be provided for implementation of various concepts into the high school classroom.

Small Scale Heterogeneity In Vegetation Structure In A Patch-Burn-Grazing Landscape, Eric G. Behrens

UNO Student Research and Creative Activity Fair

Disturbances are drivers of spatial change in the plant communities of the tallgrass prairie ecosystem. Fire and large herbivore grazing are prevalent disturbances that maintain the vegetation structure and create spatial heterogeneity in vegetation at various scales. The fire-grazing disturbance interaction occurs when areas that have been recently burned are followed by intense grazing due to the high quality of forage regrowth. This interaction has been managed in tallgrass prairies in a homogenous method using uniform burning and grazing and a heterogeneous method using patch-burn-grazing (PBG). Vegetation heterogeneity at a large scale has been studied in PBG and uniformly managed …

The Evaluation Of Carbon Dioxide-Independent Media For Toxoplasma Gondii Growth, Matthew C. Martens

UNO Student Research and Creative Activity Fair

Toxoplasma gondii is a human pathogen that plays a role as the causative agent of toxoplasmosis, a disease that produces many deleterious effects for immunocompromised individuals and infants. Current research involving this intracellular parasite requires the use and continues maintenance of costly cell incubators attached to bulky carbon dioxide canisters. To reduce laboratory costs and space requirements, we, therefore, propose a novel procedure by which human foreskin fibroblasts, as well as Toxoplasma gondii tachyzoites, may be grown in a commercially available carbon dioxide-independent media. Growth assays utilizing resazurin dye or relative fluorescence from parasites modified with a red fluorescent protein …

Social Immunity In A Sub-Social Insect: Influence Of Parental Care And Exposure To Microbes During Development, Jessica L. Rerucha

UNO Student Research and Creative Activity Fair

Exposure to microbes during development can have long-lasting impacts into adulthood. Positive effects include ontogenetic priming (i.e., enhanced immune response due to prior exposure), while negative effects include reduced growth resulting in smaller adults with reduced reproductive performance. Mitigating factors like parental care can reduce or even eliminate any fitness costs. The aim of this study was to determine i) whether exposure to microbes during development causes ontogenetic priming and ii) whether parental care mitigates long-term costs associated with exposure to microbes during development. As study organism, we used the burying beetle Nicrophorus marginatus which provide parental care to its …