Life Sciences Commons^™

Investigating Regulation Of Neutrophil-Mediated Inflammation In Mycoplasma Pneumoniae Infection, Nathan D. Velazquez

University Scholar Projects

No vaccine exists for Mycoplasma pneumoniae (Mp) infections, largely due to the

gap in understanding in the virulence and immunopathogenesis of this bacterium.

We have recently demonstrated that neutrophils are maladaptive in the context of

Mp infection, and drive disease severity while also impairing bacterial clearance.

Furthermore, we observed that B cells play a regulatory role in limiting disease

severity by modulating neutrophilic inflammation. We investigated this

phenomenon further to elucidate the mechanisms by which B cells regulate

neutrophilic inflammation. We utilized a monoclonal antibody to deplete B cells

during Mp infection and found that B-cell-depleted mice had elevated neutrophil …

Economy Smart Watch Fails To Accurately Monitor Blood Glucose Among Healthy Adults, Thinh Le Mr., Douglas Oberlin Dr., Adam Mohan Mr.

Undergraduate Research

Monitoring and maintenance of blood glucose (BG) concentrations is of critical importance to individuals with diabetes mellitus. However, this often requires multiple finger-sticks and a glucometer, or the use of a prescription continuous glucose monitor (CGM). Recently, some smart watches have become available which claim to non-invasively monitor BG. This should not be confused with watches that pair with CGMs. The purpose of this study was to assess whether the economy smart watches were capable of detecting BG changes among healthy individuals. Currently three participants have completed this study. Healthy young adults reported to the lab after a 4-6 hour …

The Impact Of Prenatal Yoga On Hip Mobility And Delivery Outcomes, Zoe Avery

Mahurin Honors College Capstone Experience/Thesis Projects

Yoga has been shown to decrease lumbar pain, improve maternal mental health, and improve delivery outcomes. While there are many well-established benefits of yoga, the mechanism by which yoga elicits these improvements is unclear. One potential mechanism that has not been studied is through improvements in hip mobility. PURPOSE: The purpose of this study was to test the effect of prenatal yoga on hip mobility. The secondary aim was to determine the relationship between hip mobility and pregnancy outcomes. METHODS: Hip mobility via hip internal and external range of motion and hip flexor muscle length was measured in pregnant females …

Differences Between Media And Industry Professionals’ Views On Welfare Issues At Equine Racetracks In The United States, Olivia Burris

Honors College Theses

The objective of this project was to see if there was a difference in views between lay publications in the media and industry professionals regarding welfare issues occurring at equine racetracks. This study focused on the issues of drug usage and racing-related injuries in racehorses. This project examined current lay publications versus surveys of industry professionals discussing the specific welfare issues. These lay publications were reviewed and summarized for comparison to the industry professionals’ responses to the survey.

A Sex Comparison On The Effects Of Caveolin Scaffolding Domain On Fibrosis Formation After Chronic Sympathetic Stimulation, Crimsyn Price

Undergraduate Honors Theses

Cardiovascular disease (CVD) is the leading cause of mortality worldwide. There are various trends that influence the rates of cardiovascular disease in each country. Factors that create these trends involve diet, exercise, genetics, socioeconomic status, and geographical location. Additionally, CVD is typically higher in middle-income and lower-income classes. Hospital routes, rates of poverty, insurance policies, and lifestyle culture are all factors that can impact the time of treatment which in turn affect the rate of CVD. However, we explored in this study the shift in CVD women experience after menopause. Women were excluded from clinical trials until 1986, and the …

Understanding The Role Of Sabp2-Interacting Proteins Sip432: A Premnaspirodiene Oxygenase Enzyme In Stress Signaling, Cristiana F. Martinez

Undergraduate Honors Theses

This study focuses on the characterization of a SABP2-interacting protein (SIP)-432. SIP432 is a putative premnaspirodiene oxygenase-like P450 enzyme. Premnaspirodiene is a 15-carbon compound that can be converted into antimicrobial compounds by a hydroxylation reaction. The interaction of SIP432 with SABP2, a critical component of salicylic acid-mediated plant immunity, implies a role for SIP432 in plant stress signaling. This study uses the T-DNA knockout mutant of Arabidopsis thaliana SIP432 (homolog of NtSIP432) to help understand its role in abiotic and biotic stress responses. Plants were subjected to abiotic and biotic stress to understand their role in stress response pathways. Gene …

Frontier Healthscapes: Competing Narratives Of Disease, Environment, And Wellbeing In Early Minnesota, Aidan Reynolds

Geography Honors Projects

Across settler societies, health narratives were shaped by evolving tensions between lay and expert knowledge, frameworks of disease causation, and perceptions of the environment as both a source of health and a threat. In the decades following Euro-American settlement (1860-1900), Minnesota became a focal point for these debates, with its landscape fostering complex and competing narratives on health, wellbeing, and disease. This paper explores how public health discourse developed in Minnesota through the perspectives of institutions, the public, and economic interests. Combining environmental history with critical discourse analysis of archival materials, this study examines shifts within the Minnesota State Board …

The Effect Corrugated Fiberboard Has On The Environment In Terms Of Compostability, Plant Growth, And Per-And-Poly-Fluoroalkyl Substance Contamination, Meredith Clem

All Theses

The “Lasagna Method” is a popular and sustainably perceived technique that many gardeners use as a free and convenient method to dispose of cardboard (corrugated fiberboard) packaging while also improving their gardens. Many claim that this creates benefits for your garden, but many others have proposed their concerns, including the safety of the practice. This research analyzes the compostability of cardboard, the effect it has on plant growth and development of lettuce, and its contamination of a prominent chemical that has known negative effects on human health. The results of these studies showed that cardboard, as it is currently produced …

Genomic Analysis Of The Lyme Disease Vector Across Virginia’S Spatial Expansion Front, Amy Jablonski

Honors Theses

Lyme disease is the most common vector-borne disease in the United States and is expanding with rising incidence in Virginia . This study investigates populations of lxodes scapularis across Virginia using RAD-seq to analyze SNPs from ticks collected in seven Virginia counties and four U.S. states as references . Results show a clear genetic split between western and eastern Virginia ticks . These results follow previously noted patterns in regional differences in Lyme disease risk.

Using A Process Evaluation In Extension Programming: Takeaways For Teams, Cristina Connolly, Jeantyl Norze, Stacey F. Stearns

Journal of Human Sciences and Extension

Health fields use process evaluations to measure how closely an intervention aligns with its intended delivery. Despite their potential for improving Cooperative Extension programming and impacts, process evaluations are not widely used outside the medical sciences. We adapted the process evaluation framework for Extension use. The components assess project components such as content, dose delivered, dose received, and reach, as well as moderating factors such as quality of delivery, participant responsiveness, complexity, facilitation strategy, recruitment, and context. The modified process evaluation framework was used to assess an Extension project in the Northeast region of the U.S. Extension teams in the …

Growing Pains: Assessing The Strategic Organizational Communication Approaches Of An Expanding Extension Organization, Anissa M. Mattox, Ashley Mcleod-Morin, Lauri Baker, Angela B. Lindsey

Journal of Human Sciences and Extension

As social structures evolve, strategic organizational communication (SOC) must also evolve for organizations to best meet the ever-changing needs of stakeholders. Assessing communications can guide the direction of an organization while also elevating the organization to the next level. For this study, researchers assessed the SOC strategy of the Extension Disaster Education Network (EDEN) through qualitative focus groups. Results of this study revealed internal communication and resources provided by the organization were perceived as positive communication approaches, while external communication and visibility were identified as negative communication approaches. Other Extension organizations could benefit from the results of this research as …

Unraveling The Causal Influences Of Drought And Crop Production On Groundwater Levels Across The Contiguous United States, Nitin K. Singh, Sheila M. Saia, Ruchi Bhattacharya, Hoori Ajami, David M. Borro

Biological, Geological, and Environmental Faculty Publications

Groundwater depletion in agricultural-dominated regions is attributed to climate and irrigation withdrawals that support crop production. However, despite decades of effort, knowledge gaps remain in understanding the relative influence of drought and crop production on groundwater levels at the continental scale. Here, utilizing empirical observations, we simultaneously track how long-term trajectories of groundwater levels, crop production of seven crops, and drought have evolved over time, and then integrate these observations with a causality-based attribution framework to unravel the relative impact of drought and crop production on groundwater levels across the contiguous United States (CONUS). We find a dominant pattern of …

An Australian Chickpea Pan-Genome Provides Insights Intogenome Organization And Offers Opportunities For Enhancing Drought Adaptation For Crop Improvement, Vanika Garg, Rutwik Barmukh, Yan Huang, Annapurna Chitikineni, Kristy Hobson, Bicheng Yang, Yong Jia, Shengnan Bi, Sukhjiwan Kaur, Muhammad Ahsan Asif, Matthew Hayden, Sally Norton, Darshan Sharma, Kadambot H. M. Siddique, Xin Liu, Chengdao Li, Rajeev K. Varshney

Grain and Other Field Crops Research Articles

Summary

Chickpea (Cicer arietinum L.) is an important legume crop that has been subjected to intensive breeding, resulting in limited genetic diversity. Australia is the world’s second largest producer and the leading exporter of chickpea; the genomic architecture of its cultivars remains largely unexplored. This knowledge gap hinders efforts to enhance their genetic potential for production, protection, and stress adaptation. To address this, we generated high-quality genome assemblies and annotations for 15 leading Australian chickpea cultivars using single-tube long-fragment read technology. The pan-genome analysis identified 34 345 gene families, including 13 986 dispensable families enriched for genes associated with key …

Cationic Conjugated Polymers With Enhanced Doped-State Planarity For N-Type Organic Thermoelectrics, Jiu-Long Li, Xin-Yu Deng, Jupeng Chen, Peng-Xiang Fu, Shuang-Yan Tian, Yunfei Wang, Xiaodan Gu, Ting Lei

Faculty Publications

To date, extensive efforts have been devoted to designing new conjugated polymers with long alkyl or ethylene glycol sidechains. However, these sidechains are insulators, limiting further performance enhancement in doped conjugated polymers. Moreover, the most widely used chlorinated solvents are toxic, limiting the practical applications of many conjugated polymers. Here, we report a water/alcohol processable n-type conjugated polymer P(Py2FT), featuring side chain-free cationic backbones. P(Py2FT) exhibits a high n-type electrical conductivity of up to 28.1 S cm⁻¹ and a high thermoelectric power factor of up to 28.7 μW m⁻¹ K⁻², comparable to some conventional …

Prevalence Rates Of Tetb, Teta, And Tetm Tetracycline Resistance Genes In Environmental Escherichia Coli Isolates From Local Waterways, Caris Cianelli

Honors Projects

The purpose of this study was to determine the prevalence rates of the tetracycline resistance genes tetA, tetB, and tetM in environmental Escherichia coli isolates. Samples were from both an urban and an agricultural waterway were filtered and incubated with Coliscan™ medium to differentiate for fecal coliforms. The resulting colonies were streaked onto EMB and then TSA plates to ensure isolation and presence of E. coli. All media used were supplemented with 10 µg/mL tetracycline in order to maintain selective pressure on the isolates. DNA from the isolates was then extracted and PCR was run using tetA, tetB, and tetM …

Characterizing The Medial Forebrain Bundle In Adolescents With Anhedonic Depression, Tina Nguyen

Biological Sciences Undergraduate Honors Theses

Adolescent depression has quickly grown in prevalence, yet the underlying neuropathology remains poorly understood. Anhedonia–the diminished ability to experience pleasure–is a core symptom of melancholic depression. Early identification of biomarkers associated with anhedonic depression could help mitigate the severity of the disorder by enhancing treatment efficacy. Blunted functionality of the nucleus accumbens (NAcc), the reward center of the brain, and abnormal dopaminergic activity in the ventral tegmental area (VTA) have both been linked to depression. Furthermore, glutamatergic input from the hypothalamus modulates the VTA which in turn, downregulates dopamine release in the NAcc. However, there is limited evidence supporting that …

The Effect Protein Supplementation On Waist-To-Hip Ratio, Bmi, Lipid Profile, And Resting Energy Expenditure In Postmenopausal Women, Caleb Flores

Biological Sciences Undergraduate Honors Theses

Postmenopausal women are a growing and at risk population that face increased risk for adverse changes in body composition, lipid profile, and overall cardiometabolic health due to hormonal changes associated with aging. Over 40% of postmenopausal women have obesity, which can be linked not just aging but the menopausal transition. Because of the negative effects of menopause on cardiometabolic, research into dietary interventions to combat these effects are needed. High-protein diets have shown promise in mitigating these effects, but sufficient research has not been done to determine the effects on postmenopausal women. This study aimed to evaluate the effects of …

Assessment Of Nitrogen Source Utilization During Nitrogen Fixation And Nitrogenase Regulation By Methanosarcina Acetivorans, Avery Carter

Biological Sciences Undergraduate Honors Theses

Nitrogen is a crucial element in biomolecules such as proteins and DNA, essential for all cellular life. Biological nitrogen fixation, a process utilized by certain bacteria and archaea, reduces dinitrogen (N₂) to ammonia (NH₃), enabling growth in the absence of fixed nitrogen sources like NH₃. This process is driven by the metalloenzyme nitrogenase, which catalyzes the reaction: N₂ + 16ATP + 8e⁻ + 8H⁺ → 2NH₃ + H₂ + 16ADP + 16Pi. Due to its high energy demand, nitrogenase production and activity are tightly regulated. Among archaea, nitrogenase is found exclusively in methanogens, where its regulation remains poorly understood. We …

The Impact Of Maternal Age On The Expression Of Transgenerational Plasticity In Daphnia Pulicaria, Calvin Nguyen

2025 Spring Honors Capstone Projects - Archive

Transgenerational plasticity refers to heritable, non-genetic changes in phenotype that persist across multiple generations and can enhance offspring survivability in variable environments. In Daphnia, increasing maternal age has been associated with maladaptive plasticity. To investigate this relationship, six clones were collected from two Wisconsin lakes and acclimated to laboratory conditions through a common garden rearing process. For each clone, ten replicates were generated and evenly divided between young (clutches 2–4) and old (clutches 5–8) maternal age groups. Offspring were exposed to three dietary treatments for three experimental generations: one fed only green algae, one fed only cyanobacteria (a nutritionally …

Rnai Pathway Homeostasis Is Essential For Longevity In C. Elegans., Sejal Dulal

2025 Spring Honors Capstone Projects - Archive

RNA interference (RNAi) is a gene regulatory mechanism that is conserved across all animal species, including humans and the microscopic nematode, Caenorhabditis elegans. Many studies have identified the RNAi pathway factors and dissected the regulatory mechanisms of RNAi pathways, but there is little understanding of how the RNAi pathways themselves are regulated. Previously, Rogers and Phillips identified a feedback loop mediated by a pair of small RNA sensors, Sensor of siRNAs-1 (sosi-1) and eri-6[e-f], embedded within the genomic locus of a small RNA biogenesis factor, ERI-6/7. In this feedback motif, loss of small RNA-mediated silencing of …

A Comparative Analysis Of Small Nuclear Rna Sequences In Spinacia Oleracea, Arabidopsis Thaliana, And Homo Sapiens, Nhi T. Nguyen

2025 Spring Honors Capstone Projects - Archive

In eukaryotes, the structure and function of the Sm core and its associated small nuclear ribonucleoproteins (snRNPs) play a significant role in pre-mRNA splicing. These snRNPs exhibit a high degree of conservation. While plants also utilize alternative splicing, the conservation of plant snRNAs and other non-coding RNA such as snoRNA and scaRNA, which assemble the Sm core, remains largely unexplored. In this study, plant sequences were compared with human sequences using the multiple sequence alignment program Jalview to determine the conservation of snRNPs in other non-coding RNA. Sequences analysis showed that the Sm site in Spinacia oleracea and Arabidopsis thaliana …

Elucidating The Role Of Transposable Element Derived Gene, Dplg3, In Drosophila Melanogaster, Kaisy A. Martinez

2025 Spring Honors Capstone Projects - Archive

A big fraction of most eukaryotic genomes is comprised of transposable elements (TEs). TEs are mobile genetic sequences often causing deleterious effects as they increase in copy number. On rare occasions, TEs can undergo molecular domestication, where the TE proteins are co-opted by the host to serve cellular functions. In Drosophila melanogaster, domestication from PIF/Harbinger TEs has resulted in five Drosophila PIF-Like Genes (DPLG1-4 and 8). This study focuses on investigating the function of DPLG3. In DPLG3 knock out lines, DPLG3-KO, a fraction of the progeny shows an abnormal gonad phenotype. We sought to confirm …

Assessing The Prevalence Of Ehrlichia Spp. In Ticks Associated With Canids In Arkansas, Eva J. Berryhill

Animal Science Undergraduate Honors Theses

Canine ehrlichiosis is a disease that is commonly seen in dogs. In previous studies, the southeastern part of the United States has been identified as an area of high prevalence. Arkansas was included in the region where the prevalence is high. Changes in climate, household income, and tick population were identified as factors contributing to this high prevalence. Canine ehrlichiosis is caused by three Ehrlichia spp., which are E. canis, E. ewingii, and E. chaffeensis. Ticks are responsible for transmitting the disease, specifically R. sanguineus, D. variabilis, and A. americanum. For this study, the goal was to determine the prevalence …

Utilizing 16s Ribosomal Rna Sequencing To Identify Bacterial Species Colonizing Lame Birds With Bacterial Chondronecrosis With Osteomyelitis, Kara Hoover

Animal Science Undergraduate Honors Theses

Broiler chicken lameness has become prevalent in recent years due to high growth rates in broilerchickens. Bacterial chondronecrosis with osteomyelitis (BCO), the main cause of lameness, is characterized by necrosis of femoral and tibial heads in affected birds. Heavier birds are more susceptible to mechanical stress in these regions, which results in microfractures, leading to lameness. BCO lameness poses huge financial pressure to the poultry industry, as 14-50% of flocks can become affected during outbreaks. The purpose of this experiment was to identify bacterial species present in birds with BCO lameness. A total of 1,560 chicks were randomly distributed into …

The Effect Of 1,25-Dihydroxyvitamin D3 Supplementation On Humoral Immunity Against Bacterial Chondronecrosis With Osteomyelitis Lameness In Broiler Chickens, Bailey Herberger

Animal Science Undergraduate Honors Theses

Bacterial chondronecrosis with osteomyelitis (BCO) lameness, the most predominant locomotive illness in fast-growing broilers, causes substantial animal well-being issues and affects broiler production, leading to huge financial losses. Several strategies, including feed supplementations using organic trace minerals, vitamin D, probiotics, and prebiotics, have been utilized to alleviate BCO lameness incidences in broiler chickens. Previous studies have indicated that feeding an additive of Panbonis^®-G-1,25(OH)₂D₃ from Solanum glaucophyllum containing 1 µg/kg G-1,25(OH)₂D₃ to broilers for the first 28 days of their production cycle reduced BCO lameness by more than 50%. In this study, we …

Effects Of Common Herbicides On Organismal Interactions, Robert Chamberlin

All Theses

Pesticides are applied to agricultural lands to prevent undesirable weeds, animal pests, and fungi from harming crops and reducing crop yields. Pesticides are used in croplands at increasing rates around the world and have become an essential component of farming in recent decades. Often, rain, wind, and soil permeation move pesticides from farmlands to natural environments. Such contamination of natural environments by pesticides may threaten species by altering their behaviors, bodily functions, and the ways in which individuals interact. Atrazine and glyphosate are among the most widely used herbicides around the world and are useful in eliminating weeds, primarily in …

Uncovering The Evolutionary Origins Of Neo-Sex Chromosomes In The Insect Family Membracidae, Mary Kumah

2025 Spring Honors Capstone Projects - Archive

This research explores the evolution of the neo-XX/XY sex chromosome system of Amblyophallus exaltatus, a treehopper insect in the family Membracidae. Neo-XX/XY sex chromosome systems can evolve from an XX/X0 sex chromosome system via fusion events between the X chromosome and an autosome. Although cytological work has indicated the presence of a neo-XX/XY system within A.exaltatus, the chromosomal identity of the fused autosome and the molecular drivers behind this chromosomal fusion remain poorly understood. By performing a coverage analysis with whole genome sequence data, the parts of the A.exaltatus genome that fused with the X chromosome will be better …

Activity-Based Protein Profiling On Nucleophilic Amino Acid Residues With Pyrocarbonates, James Van Horn

Chemistry & Biochemistry Undergraduate Honors Theses

The complexity of the proteome requires strategies that efficiently characterize protein functions. Activity-Based Protein Profiling (ABPP) is a method that identifies enzyme activity by targeting key catalytic residues. This study focused on histidine, a residue involved in enzymatic catalysis, metal coordination, and protein-protein interactions. Despite its importance, histidine-specific chemical probes are limited due to its variable reactivity. This work developed and characterized a histidine-specific probe using a pyrocarbonate-based mechanism. Singleresidue assays confirmed selective reactivity of the probe with histidine over other nucleophilic amino acids. Further testing on enzymatic proteins and lysed cell proteomes showed that the probe modified histidine residues …

Bioavailability Of Lead In Terre Haute Soils, Terre Haute, Indiana, Zach Kemp

All-Inclusive List of Electronic Theses and Dissertations

Contamination of soils from heavy metal pollution has been a global concern for decades. Heavy metals are released into the soil from natural and anthropogenic releases. The largest producers of heavy metal pollution include mining, industrial production, and the combustion of fossil fuels. Vigo County formed in 1818, and Terre Haute was established in 1832. After the discovery of coal in Clay County in 1867, Terre Haute became the third largest coal producer and fifth largest iron manufacturer in the state during that time. Lead, in particular, is a heavy metal of concern because of the harmful effects to humans …

A Review On Treatment, Prevention, And Research Of Worldwide Parasitic Disease: Toxoplasmosis, Hannah Skinner

All Theses

Toxoplasmosis is a worldwide, yet often overlooked, parasitic disease caused by the protozoan parasite, Toxoplasma gondii. The parasite is capable of infecting humans and almost all warm-blooded vertebrates. Over a million people are infected in the United States with toxoplasmosis each year. Most infections are asymptomatic but severe cases can lead to vision impairment, neurological disorders, and congenital birth defects. T. gondii is transmitted through undercooked meat, infected feline feces, or contaminated water and soil. The burden of T. gondii infection is most notable in livestock, felines, and immunocompromised humans. Despite being a widespread disease, toxoplasmosis is neglected in …