Life Sciences Commons^™

Genetic Code Expansion In Biochemical Investigations And Biomedical Applications, Nanxi Wang

Department of Chemistry: Dissertations, Theses, and Student Research

Genetic code expansion provides a powerful tool for site-specific incorporation of unnatural amino acids (unAAs) with novel biochemical and physiological properties into proteins in live cells and organisms. To achieve this, a nonsense codon suppression system, which consists of an orthogonal aminoacyl-tRNA synthetase (aaRS) and tRNA pair that specifically decodes a nonsense codon (e.g., amber codon and quadruplet codon) with an unAA but do not “cross talk” with their endogenous counterparts, was established. This Ph.D. thesis presents our efforts on evolution and application of nonsense codon suppression systems for biochemical and biomedical investigations.

In Chapter 1, a brief overview of …

Carisbamate Blockade Of T-Type Voltage-Gated Calcium Channels, Do Young Kim, Fang-Xiong Zhang, Stan T. Nakanishi, Timothy Mettler, Ik-Hyun Cho, Younghee Ahn, Florian Hiess, Lina Chen, Patrick G. Sullivan, S. R. Wayne Chen, Gerald W. Zamponi, Jong M. Rho

Spinal Cord and Brain Injury Research Center Faculty Publications

Objectives

Carisbamate (CRS) is a novel monocarbamate compound that possesses antiseizure and neuroprotective properties. However, the mechanisms underlying these actions remain unclear. Here, we tested both direct and indirect effects of CRS on several cellular systems that regulate intracellular calcium concentration [Ca²⁺]_i.

Methods

We used a combination of cellular electrophysiologic techniques, as well as cell viability, Store Overload‐Induced Calcium Release (SOICR), and mitochondrial functional assays to determine whether CRS might affect [Ca²⁺]_i levels through actions on the endoplasmic reticulum (ER), mitochondria, and/or T‐type voltage‐gated Ca²⁺ channels.

Results

In CA3 pyramidal neurons, kainic …

A Program Aimed Toward Inclusive Excellence For Underrepresented Undergraduate Women In The Sciences, Laura A. Katz, Kathryn M. Aloisio, Nicholas J. Horton, Minh Ly, Sara B. Pruss, Kate Queeney, Cate Rowen, Patricia Marten Dibartolo

Biological Sciences: Faculty Publications

Created to foster inclusive excellence, Smith College’s Achieving Excellence in Mathematics, Engineering, and Science (AEMES) Scholars program provides early faculty-mentored research opportunities and other programming as a way to foster success in academic outcomes for underrepresented women in science. Using academic record data, we compared Scholars’ outcomes over time with those of underrepresented students before program launch and to relevant peer comparison groups. Since its launch, AEMES Scholars have achieved significantly higher gateway life sciences course grade point averages (GPAs), rates of persistence in life and natural sciences, and participation in natural sciences advanced research relative to baseline. Gains for …

Climate Change, Managed Relocation, And The Risk Of Intra-Continental Plant Invasions: A Theoretical And Empirical Exploration Relative To The Flora Of New England, Jesse Bellemare, Bryan Connolly, Dov F. Sax

Biological Sciences: Faculty Publications

The high rate of anthropogenic climate change projected for coming decades and evidence of low migration ability for many species have led researchers to warn of a looming extinction crisis. This threat is expected to be most acute for small-ranged endemic species, which could see novel climatic conditions develop rapidly across the entirety of their limited geographic ranges. To avoid extinctions, some conservationists have proposed that climateimperiled species might be candidates for "assisted colonization" or "managed relocation" to new regions, outside their historical ranges. One major concern related to managed relocation is the possibility that some relocated species could later …

Differential Toxic Effects Of Ulva Lactuca (Chlorophyta) On The Herbivorous Gastropods, Littorina Littorea And L. Obtusata (Mollusca), Paulette Peckol, Alysha B. Putnam

Biological Sciences: Faculty Publications

Members of the genus Ulva are widespread and abundant in intertidal and shallow subtidal areas but there are conflicting data regarding susceptibility to herbivory. While some studies have documented that Ulva spp. were favored by a diversity of marine herbivores, other work has revealed herbivore deterrence. We investigated grazing and growth rates of the littorinid species, Littorina littorea and L. obtusata, when offered Fucus vesiculosus, Ascophyllum nodosum, Ulva lactuca, and Chondrus crispus, highlighting distinctive vulnerabilities to toxic effects of U. lactuca. Ulva lactuca was the preferred food of L. littorea, while L. obtusata showed no grazing on this ephemeral algal …

Emerging Antimicrobial Research Against Superbugs: Perspectives From A Polymer Laboratory, Mitra S. Ganewatta, Md Anisur Rahman, Chuanbing Tang

Journal of the South Carolina Academy of Science

Infectious diseases caused by drug-resistant microorganisms have become a major contributor for human morbidity and mortality. To overcome such threats, we have developed various antimicrobial agents using natural product derivatives and metallopolymers. Abundant biomass such as resin acids can be utilized to prepare cationic polymers for inhibiting a variety of bacteria. These polymers have been used in solution as well as surfaces as antimicrobial materials with low cytotoxicity. In addition, a class of charged metallopolymers have been developed to kill superbugs such as MRSA.

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Antitumor Activity Of Selected Derivatives Of Pyrazole- Benzenesulfonamides From Dilithiated C(Α), N-Phenylhydrazones And Lithiated Methyl 2-(Aminosulfonyl)Benzoate, N. Dwight Camper [Deceased], James M. Gum, Darby E. Lyles, William T. Pennington, Charles F. Beam, Clyde R. Metz

Journal of the South Carolina Academy of Science

Several pyrazole-benzenesulfonamides were subjected to biological evaluation involving tumor formation on potato discs caused by Agrobacterium tumefaciens. This assay led to some excellent and promising initial results with three of the pyrazole compounds showing increased tumor inhibition when compared to a recognized standard, camptothecin. The select pyrazole-benzenesulfonamides were prepared by condensation-cyclization of several dilithiated C(α),N-phenylhydrazones with lithiated methyl 2-aminosulfonyl-benzoate.

Ecological Correlates With Dioecy In The Flora Of A Tropical Premontane Wet Forest In Costa Rica., Breanne Held

Undergraduate Distinction Papers

There is a considerable disagreement about the selective forces that have led to the evolution of dioecy (i.e., the condition of having separate male and female flowers on separate plants). Historically, selection to enforce outcrossing has been the primary explanation despite there being little empirical evidence. Hence, many scientist argue that the selection of dioecy is driven by ecological traits that influence male and female fitness and seed dispersal. The objective of this study was to examine the association between plant sexual system (particularly, dioecy) with various ecological traits for the flora of a tropical premontane wet forest in Costa …

Using Murine Models To Understand Tumor-Lymphoid Interactions: Spotlight On Cll And Aitl, Tyler Herek, Christine E. Cutucache

Biology Faculty Publications

The role of the tumor microenvironment in leukemias and lymphomas is well-established, yet the intricacies of how the malignant cells regulate and influence their non-malignant counterparts remain elusive. For example, chronic lymphocytic leukemia is an expansion of malignant CD5+CD19+ B cells, yet the non-malignant T cells play just as large of a role in disease presentation and etiology. Herein, we review the dynamic tumor cell to lymphoid repertoire interactions found in two Non-Hodgkin’s lymphoma subtypes: chronic lymphocytic leukemia and angioimmunoblastic T-cell lymphoma. We aim to highlight the pivot work done in the murine models which recapitulate these diseases and explore …

They Just Need To Move: Teachers' Perception Of Classroom Physical Activity Breaks, Danae Dinkel, Connie Schaffer, Kailey Snyder, Jung Min Lee

Health and Kinesiology Faculty Publications

The purpose of this study was to explore 1) perceptions of preschool-8th grade teachers' in regard to classroom physical activity (PA) and 2) multiple levels of factors impacting preschool-8th grade teachers' ability to implement PA into the classroom. Sixty preschool-8th grade teachers from five school districts participated in semi-structured interviews following a guide developed from constructs of the social ecological model. All teachers implemented classroom PA but had varied levels of confidence for implementation. Teachers identified barriers to implementation and requested additional classroom PA resources. Furthermore, they identified collaboration with other teachers as an underutilized resource for promotion of classroom …

Research: South Dakota State University, Spring 2017, Christie Delfanian, Emily Weber

State of Discovery South Dakota State University's Research Magazine

Pharmacy researcher investigates how aspirin kills cancer cells [page] 1
Problem-solving, cornerstone of SDSU research [page] 2
Fluid flow model evaluates clotting risk in new stent graft design [page] 2
Sanford research project inspires graduate student [page] 3
Experience key to nurses' comfort in communicating with terminally ill patients [page] 4
Nondigestible starch feeds gut microbes, reduces inflammation [page] 5
Researchers investigate workings if antibody therapeutics [page] 6
DNA, RNA sequencing available in campus to researchers statewide [page] 7
QUBIC-R software helps researchers model, visualize gene expression networks [page] 8
Scientists assess survival of swine viruses in imported feed ingredients …

Ebola Virus - Epidemiology, Diagnosis, And Control: Threat To Humans, Lessons Learnt, And Preparedness Plans - An Update On Its 40 Year's Journey, Raj Kumar Singh, Kuldeep Dhama, Yashpal Singh Malik, Muthannan Andavar Ramakrishnan, Kumaragurubaran Karthik, Rekha Khandia, Ruchi Tiwari, Ashok Munjal, Mani Saminathan, Perumal Arumugam, Sunil Kumar Joshi

Bioelectrics Publications

Ebola virus (EBOV) is an extremely contagious pathogen and causes lethal hemorrhagic fever disease in man and animals. The recently occurred Ebola virus disease (EVD) outbreaks in the West African countries have categorized it as an international health concern. For the virus maintenance and transmission, the non-human primates and reservoir hosts like fruit bats have played a vital role. For curbing the disease timely, we need effective therapeutics/prophylactics, however, in the absence of any approved vaccine, timely diagnosis and monitoring of EBOV remains of utmost importance. The technologically advanced vaccines like a viral-vectored vaccine, DNA vaccine and virus-like particles are …

Tumor Necrosis Factor Alpha-Induced Recruitment Of Inflammatory Mononuclear Cells Leads To Inflammation And Altered Brain Development In Murine Cytomegalovirus-Infected Newborn Mice, Maria C. Seleme, Kate Kosmac, Stipan Jonjic, William J. Britt

Physical Therapy Faculty Publications

Congenital human cytomegalovirus (HCMV) infection is a significant cause of abnormal neurodevelopment and long-term neurological sequelae in infants and children. Resident cell populations of the developing brain have been suggested to be more susceptible to virus-induced cytopathology, a pathway thought to contribute to the clinical outcomes following intrauterine HCMV infection. However, recent findings in a newborn mouse model of the infection in the developing brain have indicated that elevated levels of proinflammatory mediators leading to mononuclear cell activation and recruitment could underlie the abnormal neurodevelopment. In this study, we demonstrate that treatment with tumor necrosis factor alpha (TNF-α)-neutralizing …

Assessing A Punching Bag Feedback Performance Device, Neil Deochand

Dissertations

Physical exercise has been integrated into treatment efforts in reversing the number of overweight and obese individuals. Furthermore, exercise extends mortality, enhances general quality of life, and it is a protective health factor for preventing the progression some mental health disorders. Electronic athletic training equipment easily allows monitoring of real-time physical activity, and enables tracking of progress made toward individualized performance goals. There are limitations to only using visual feedback (e.g., visual depictions of heart rate, speed, distance traveled, or calories burned etc.) to track and improve exercise and athletic performance, especially for some sports, such as boxing. This issue …

Quantifying Cyanide Inhibition Of Nitrification And Developing Cost-Effective Treatment Processes, Germano M. Salazar-Benites

Civil & Environmental Engineering Theses & Dissertations

All wastewater treatment plants that operate multiple hearth furnaces (MHF) and are required to nitrify must manage the inhibitory effects of free cyanide (HCN, CN-) in the scrubber return flows due to inhibitory impacts on nitrifying bacteria.

HRSD Boat Harbor Treatment Plant (BHTP) a 25 MGD facility consisting of primary and secondary treatment, employs an anoxic selector process for nitrification and partial denitrification and operates a MHF. There is a desire to improve TN removal performance at BHTP due to an annual mass-based bubble permit limit on a combined discharge from seven HRSD plants, and there are no discharge limitations …

Strawberries (Frag X Anan) Are Bigger When Native Mason Bees (Osmia Lignaria) Are Experimentally Added To Small Farms, Laura A. Campbell

Biological Sciences Theses & Dissertations

Agriculture is the cornerstone of our economy, and pollination plays a vital role in nearly all non-wind pollinated crops. Declining honey bee populations have caused a more diligent investigation into using solitary bees for crop pollination. The native, solitary Orchard Mason Bee has shown to successfully pollinate almonds and apples, but there has been little work conducted on their effectiveness as a low fruit crop pollinator. This study assesses their effectiveness as a small strawberry farm pollinator, and preferences for nesting materials. Mason bee cocoons and nest homes made with different materials (bamboo, Phragmites, or wood) were supplemented on …

Impact Locations And Damage To Civil And Military Rotary-Wing Aircraft From Wildlife Strikes, Brian E. Washburn, Paul Cisar, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Rotary-wing aircraft (e.g., helicopters and tilt-wing aircraft) are an important component of all U.S. military services and the U.S. civil aviation industry. Our analyses of wildlife strikes to military rotary-wing aircraft, both within the United States and during overseas deployments, as well as civil helicopters, have shown there are important patterns within wildlife strike data for flight operations conducted on airfields and during off -airfield missions. Birds accounted for 93% of the wildlife strikes where the animal was identified, and mammals (primarily bats) accounted for 7%. Wildlife impacted all parts of civil helicopters and military rotary-wing aircraft during strike events; …

Inventory Of Wildlife Use Of Mortality Pits As Feeding Sites: Implications Of Pathogen Exposure, Jeremy W. Ellis, Susan A. Shriner, Hailey E. Mclean, Lauren Petersen, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

To better understand the use of mortality pits by wildlife and possible pathogen dissemination from the resulting wildlife contact in these areas, we used 8 camera traps on 4 mortality pits in Colorado from June to December 2014 to create a species inventory and establish use estimates for those species. We observed 43 species visiting (in or near) the mortality pits during 1,168 total camera trap days. Of these, 24 species directly interacted with the mortality pits or carcasses contained within them. The most common visitors to mortality pits were raccoons (Procyon lotor), coyotes (Canis latrans), …

Effects Of Gm Disclosure Statements On Consumer Perceptions Of Selected Food Products In Survey And Sensory Panel Settings, Ellyn Margaret Newcomb

Theses and Dissertations

The National Bioengineered Food Disclosure Standard (PL 114-216) will require nearly all foods sold in the U.S. to bear a statement disclosing whether they contain genetically modified (GM) material. Past studies suggest the presence of such a statement could have profound effects on consumers; however, research comparing consumer response towards different GM-disclosure statements is scarce. PL 114-216 states that GM foods shall not be considered more or less safe than their non-bioengineered counterparts, nevertheless it would benefit regulators and food manufacturers to be aware of the possible effects such disclosures might have on consumers. In a nationwide survey, multiple disclosure …

Are Cardiovascular Disease Inflammatory Markers Elevated In Those With Nonspecific Chronic Musculoskeletal Pain Compared To Nonpain Case Controls?, Jeffrey Ray Tolley

Theses and Dissertations

CONTEXT: Recent studies have considered the role of inflammation in the development of both cardiovascular disease (CVD) and musculoskeletal conditions, such as rheumatoid arthritis. Studies suggest that inflammation plays a significant role in the development of cardiovascular disease. In conditions of chronic pain, as with rheumatoid arthritis, inflammation has also been noted through elevated levels of inflammatory markers. There are currently no studies that examine the possible connection between inflammatory markers related to increased risk of cardiovascular disease and nonspecific chronic musculoskeletal pain (NCMP). OBJECTIVE: The purpose of this study was to determine whether urinary levels of microalbumin (MA) and …

Ecological And Genetic Variation Among Populations Of Boechera Caeruleamontana Sp. Nov. (Brassicaceae) From Blue Mountain And Dinosaur National Monumentin Eastern Utah And Western Colorado, Melissa Snyder

Theses and Dissertations

Boechera is a large genus of flowering plants whose taxa are found primarily in North America. Boechera vivariensis (S.L. Welsh) W.A. Weber (the Park rockcress) is restricted to the Uintah Basin on Weber sandstone substrates in the vicinity of Dinosaur National Monument and Blue Mountain. The nomenclature of Park rockcress is significantly impacted by the discovery that the type collections of the taxon represent a rare, apomictic diploid resulting from the hybridization between B. thompsonii and an undescribed sexual diploid (to be called Boechera caeruleamontana sp. nov. Allphin and Windham). As a result, greater information is needed regarding how B. …

The Antipredator Behaviors Of Cryptic And Aposematic Anurans And Variability In Alkaloids, Conspicuousness, And Pattern Within Dendrobates Auratus (Dendrobatidae), Annelise Blanchette

Masters Theses

Crypsis and aposematism are common antipredator strategies that have evolved as defensive mechanisms to prevent predation. Prey that employ these defensive strategies also exhibit antipredator behaviors meant to avoid or deter predation. These behaviors include: (1) escape or immobility in the presence of an immediate predator or (2) exhibiting bold behavior by accepting the risk of potential predation in a novel environment in exchange for the benefits of foraging and mating opportunities. In this study, the escape and bold behaviors of cryptic members of Craugastor and the aposematic dendrobatid Dendrobates auratus were tested to compare these alternative antipredator strategies. Craugastor …

Effects Of Ultrasound On Amyloid Beta 42 (Aβ42) Mediated Neurodegeneration, Sarah Byrne

Honors Theses

Alzheimer’s disease (AD) is an age related progressive neurodegenerative disease. The exact mechanisms that lead to cell death are not entirely understood. It has been shown that accumulation of amyloid-beta-42 (Aβ42) plaques generated by mis-cleavage of amyloid-precursor-protein is the cause of neurodegeneration seen in AD. This is due to the hydrophobic nature of Aβ42 due to extra two amino acids added to the typical and naturally occurring Aβ40 in the body. These Aβ42 plaques trigger neuronal death because of the toxic nature and stress they exert on the neurons. In this study, Drosophila melanogaster transgenic model where human Aβ42 coding …

Pravastatin™ Suppresses Venous Shear Stress Dependent Induction Of Aquaporin 1 Protein Expression In Human Umbilical Vein Endothelial Cells In Vitro, Raphael J. Crum

Honors Theses

Background: Aquaporins (AQPs) are transmembrane water channels that facilitate osmotically-driven transmembrane water flux. AQP1 is abundantly expressed in vascular endothelium, where it functions in endothelial cell migration, wound healing, and cell volume regulation in response to mechanical stimuli. AQP1 protein abundance is enhanced in cultured human umbilical vein endothelial cells (HUVECs) in response to fluid shear stress. Statins, a pharmacological class of HMG-CoA reductase inhibitors, suppress the early development of vein graft intimal hyperplasia by improving endothelial cell function, most likely by affecting endothelial response to arterial shear stress. Statins may also differentially regulate the expression of specific AQPs by …

Seasonal Changes In Physical, Chemical, And Biotic Factors In Silver Lake, Ohio, Jacob J. Clancy

Honors Theses

The chemical makeup of a body of water can vary greatly depending on what kind of lake it is, the time of year and what kind of runoff enters the water. There are many abiotic factors that make up the water chemistry of a lake such as nutrient availability (Nitrogen and Phosphorous), pH, temperature, oxygen content and conductivity. Each of these factors plays important roles in the successes of many organisms that reside in the lake. The many species of zooplankton and phytoplankton thrive in different water chemistry conditions. Silver Lake is a unique lake in Ohio, because it was …

Does Riparian Forest Invasion By The Exotic Shrub Amur Honeysuckle Influence Nutrient Dynamics In Headwater Streams?, Charlotte Anne Shade

Honors Theses

Lonicera maackii (Amur honeysuckle) invasion is extensive in forests across much of Ohio and the Midwest. Amur honeysuckle has been shown to influence headwater streams and its organisms, which depend on a certain water chemistry to survive. Little has been done to understand how honeysuckle affects water chemistry and nutrient cycling. As honeysuckle canopies prevent native organic matter from entering the streams below, while also adding its own organic matter that is high in nitrogen and phosphorus, and low in lignin, the amount and types of nutrients present in both forests and streams may be significantly altered. Over a one-year …

The Role Of Adult Fiddler Crab Environmental Acoustic Cues And Chemical Cues In Stimulating Molting Of Field-Caught Megalopae, Emily E. Waddell, Wendy Dow Piniak, Kathleen A. Reinsel, James M. Welch

Student Publications

In mid-Atlantic estuaries, three fiddler crab species, Uca pugilator, Uca pugnax and Uca minax co-occur, with their adults occupying different habitat types distinguished by salinity and sediment size. Some evidence exists that selective settlement is responsible for this separation but the mechanism is largely unknown. We tested the hypothesis that field-caught megalopae would accelerate metamorphosis in the presence of adult species-specific environmental acoustic cues and conspecific chemical cues. We placed megalopae in seawater with and without adult chemical cues, exposed them to one of three sound treatments for 8 days, and recorded the time each megalopa took to metamorphose. In …

Use Of A Novel Oleaginous Microorganism As A Potential Source Of Lipids For Weanling Pigs, Jeffery A. Carroll, Ty B. Schmidt, T.R. Callaway, J.G. Wilson, Janet R. Donaldson

Faculty Publications

Weanling pigs are at risk of succumbing to illness due to an immature immune system and insufficient supply of available energy at the time of weaning. This study was aimed at determining whether oleaginous bacteria could serve as a source of lipids to weanling pigs. Weanling pigs were provided a daily dose of 1×109 colony fomring unit (CFU) = kg−1 of the novel oleaginous Enterobacter cloacae strain JD6301 or JD8715 (which is a variant form of JD6301 capable of producing extracellular triglycerides) via oral gavage for 5 d. Serum was collected every 6 h and intestinal samples were collected at …

Social Buffering In A Eusocial Invertebrate: Termite Soldiers Reduce The Lethal Impact Of Competitor Cues On Workers, Li Tian, Evan L. Preisser, Kenneth F. Haynes, Xuguo Zhou

Entomology Faculty Publications

While the impact of predator‐induced stress on prey has received considerable attention, there has been far less research into the effect of competitors. Cues from aggressive competitors should be particularly likely to evoke behavioral and/or physiological responses, since they may be indicative of both direct (interference) and indirect (exploitative) threats. The danger posed by such competitors, and the “fear” they evoke, should be reduced at lower competitor densities and by the presence of individual conspecifics specialized for defense. We assessed how Reticulitermes flavipes termite workers and soldiers were affected by cues from conspecific nestmates, conspecific non‐nestmates, and the heterospecific competitor …