Life Sciences Commons^™

Effect Of Endophytic Fungus Infection, Water Stress And Genotype On K, Ca And Mg Concentration Of Fescue, S R. Wilkinson, D P. Belesky, W C. Stringer

IGC Proceedings (1977-2023)

Replacement of endophyte infected tall fescue with endo­phyte free tall fescue is recommended to avoid tall fescue toxi­cosis. Fribourg et al. (1988) suggest that the endophyte may enhance tolerance of tall fescue to environmental stresses, including drought. This controlled environment study was undertaken to determine the effect of water stress on the K, Ca and Mg concentration of four genotypic selections of tall fescue with and without fungal endophytes. This paper emphasizes water stress and endophyte effects. The growth characteristics are reported elsewhere (Belesky et al. 1989).

Effect Of Fertilizers On Cutting On Time On Chemical Composition Of Sward From Permanent Meadow, A Golob, Zdenka Berloznik, M Leskosek, Vekoslava Stibilj

IGC Proceedings (1977-2023)

Ecological problems and surpluses of milk and meat have given rise to less intensive systems of grassland utilisation. One of such system is utilisation of air nitrogen via legume sym­biosis with nitrogen fixing bacteria. In Slovenia, where conditions for legume growth are more favourable than in NW Europe, natural meadows fertilized with P and K only give reasonable yields - on average around 7 t DM per ha (Leskosek, 1981). Most legumes suffer after fre­quent defoliation so such meadows should be defoliated only 2- 3 times during the growing season. Under less frequent cutting regime nutritive value of forage is …

The Effect Of N And K Fertilizers On The No3 Content Of Different Grass Species And Mixtures, A Szodfridt, J Varga

IGC Proceedings (1977-2023)

The major quantity of the annual grassland vegetation comes from the first cut in Hungary because of the continental climate of the country. Next to the energy content of grasses it is important to have informations about the amount of some possible toxic components such as nitrate. The critical NO3- value is considered to be 0.25-0.31 % in DM (Andreev, 1981), NO3 content higher than 0.6 % in DM is toxic (Kemp et al., 1977). Ebert et al. (1977) showed that 120 kg/ha N given for the 1st cut did not raise NO3 content higher than the critical value. K …

Determination Of Condensed Tannins In Tropical Legume Forages, L Telek

IGC Proceedings (1977-2023)

The effect of condensed tannins (proanthocyanidins) in ruminant nutrition is complex and needs further interdiscipli­nary reinvestigations with improved analytical methods. With the « cell rupture » theory of legume pasture bloat (Howarth et al., 1978), the role of condensed tannins seems to be secondary; they are acting as protein precipitants (Goplen et al., 1980) and inhibiting microbiological proliferation in cell­ular spaces (Howarth et al., 1982). Their assumed interference with digesting enzymes in vivo is doubtful (Mole and Water­man, 1985). On the importance of condensed tannins in nutrition, several reviews were published (McLeod, 1974; Tempel, 1982; Kumar and Singh, 1984 and …

Screening Sericea Lespedeza Germplasm For Tannin Concentration In The Leaves And Herbage Palatability, J C. Petersen, J A. Mosjidis, N S. Hill

IGC Proceedings (1977-2023)

Several million hectares in the subtropical world are currently used as grazing land for beef and dairy cattle, how­ever, in many cases animal production is very low. To improve animal nutrition, cultivated legume species can be introduced. Sericea lespedeza has potential and could be utilized in the acid soils of the· subtropics. Generally, sericea is low in palatability and nutritive value because its leaves contain tannins, which have long been associ­ated with low intake and reduced digestibility. Tannins are responsible for an astringent or bitter taste caused by the pre­cipitation of salivary glycoprotein (Goldstein and Swain, 1963). Chopping or macerating …

The Effect Of Selenium Fertilizer On The Selenium Status Of Sheep Grazing Annual Pasture In South-Western Australia, B R. Whelan, D W. Peter, N J. Barrow, D J. Buscall, V C. Cox

IGC Proceedings (1977-2023)

White muscle disease was first reported in grazing livestock in Western Australia by Gardiner (1961). Sheep affected by the disease could be divided into two categories : young suckling lambs in winter and early spring, and weaners and hoggets in summer (Gardiner et al., 1962). Subsequent investigations showed that, while there were few clinical symptoms of sel­enium deficiency, there were probably many sheep suffering from ill-thrift. This sub-clinical deficiency caused reduced liveweight gains, reduced wool production and lowered ewe fertility. It was most noticeable in areas of greater than 600 mm of annual rainfall on the lighter sandy soils with …

Synthesis Of Cell Wall Components In Maize Internodes, J H. Cherney, J J. Volenec, G A. Brown

IGC Proceedings (1977-2023)

Cell wall structure affects intake and digestibility of forages by rummants. Before microbial degradation of cell walls can be understood, it will be necessary to have a better understanding of cell wall formation. Internodes of grasses elongate in a_cropetal succession, with two internodes usually elongating s1multaneously. Elongating internodes consist of three regions : 1) a zone of cell division, 2) a zone of cell elongation, and 3) a zone of secondary cell wall deposition. This tissue provides an excellent system for studying cell wall changes in rapidly developing tissues. Our objectives were to : 1) examine changes in cell wall …

Natural And Artificial Drying Effects On Condensed Tannins Of Sericea Lespedeza, T H. University Of Georgia, W R. Windham, J J. Evans, C S. Hoveland

IGC Proceedings (1977-2023)

Sericea lespedeza [Lespedeza cuneata (Dumont-Cours.) G. Don.] is well adapted to acid soils and low fertility but has generally been considered a low-quality forage due to high levels of tannin and indigestible fiber. Tannin levels increase during midsummer (Windham et al. 1988). The most com­monly used assay for tannin in sericea is the vanillin-HCl pro­cedure (Burns, 1963) on oven-dried samples. Oven-drying has been reported to cause increased polymerization or oxidative changes in tannins (Goldstein and Swain, 1963). Fresh-frozen sericea contained higher amounts of extract­able tannin than field-dried forage and fiber digestibility of high-tannin sericea by sheep was much lower …

Volume 11 Full Text, Bjur Staff

Butler Journal of Undergraduate Research

No abstract provided.

Unpaired Virtual Histological Staining Of Tissue From Autofluorescence Using Regularized Cycle-Consistent Adversarial Networks, Zhesi Wen

Theses and Dissertations

We present a regularized CycleGAN with a Dense Residual U-Net to virtually stain autofluorescence images of tissue into H&E-like images. Our method outperforms standard architectures, reduces artifacts, and achieves superior FID scores, enabling efficient, label-free, and accurate digital pathology for unpaired datasets using multi-channel fluorescence inputs.

Microbial Community And Water Quality Assessment Of Crassostrea Virginica Habitats In Southeast Florida, Stacy Brown

All HCAS Student Capstones, Theses, and Dissertations

Eastern oysters (Crassostrea virginica) are essential habitat-forming species that contribute significantly to coastal water quality due to their filter-feeding behavior. Unlike other coastal regions in the Gulf of Mexico and Northeastern United States, oysters are not commercially managed in Southeast Florida and as a result sparse data exists on locations of the Eastern oyster habitat. Similarly, the benefits of oyster reefs to water quality are also not fully documented in Southeast Florida. Although beneficial and often referred to as keystone habitat building species, populations of C. virginica are not currently listed as protected species so little work has …

Using Satellite Tracking And Stable Isotopes To Characterize The Movements, Core-Use Areas, And Distribution Of Loggerhead And Green Turtles Nesting In South Florida, Usa: Internesting, Foraging, And Migration, Glenn D. Goodwin

All HCAS Student Capstones, Theses, and Dissertations

The movements of animals in response to environmental variation influence the fitness of individuals and drive major ecological processes, while also varying their exposure level to anthropogenic risks. Understanding the movements and spatial distribution of imperiled species is critical for developing effective conservation strategies. This can be difficult for wide ranging and highly migratory species, such as sea turtles. Throughout their adult life stages, the females of most sea turtle species spend the majority of their time in their foraging areas for multiyear intervals between nesting periods. These foraging areas are typically widely dispersed and variable within nesting populations. To …

Examining Loss Of Imprint In F1 Hybrid Female Mice Due To X Chromosome Epimutation And The Implications For Autism, Arianna H. Roach

Honors Scholar Theses

Sex biases are prevalent among various neurodevelopmental disorders, with males experiencing them at higher frequencies or severities than females. This male bias is poorly understood, and our lab aims to elucidate this mechanism using our model of transgenerational epigenetic inheritance. Our lab identified the cluster of genes Xlr3b/4b/4c on the X chromosome that are imprinted in the female brain. Preliminary studies suggest that Xlr3 acts as a mediator molecule in our model. Xlr3 knockdown male mice exhibited significant meiocyte loss that can be attributed to Meiotic Sex Chromosome Inactivation. Their female offspring also displayed loss of imprinting of Xlr3 and …

The Interaction Between Physical Activity And Fruit And Vegetable Intake On Body Composition, Om S. Ghetia

Honors Scholar Theses

Objective: Fruit and Vegetable (FV) intake and Physical Activity (PA) are important lifestyle factors associated with body composition. This study assessed the combined impact of meeting FV intake and PA recommendations on body composition measures including body mass index (BMI), percent body fat (PBF), and waist circumference (WC) in adults aged 20–64 years from diverse backgrounds.

Design: A cross-sectional study conducted in Northeastern Connecticut.

Methods: A total of 124 participants completed a one-week dietary and PA record followed by anthropometric assessments. Participants were categorized into groups based on FV intake (≥400 g/day vs. < 400 g/day) and PA levels (≥600 MET-minutes/week vs. < 600 MET-minutes/week). Independent t-tests and chi-square tests were used to compare dietary intake, PA levels, and body composition outcomes across groups.

Results: Participants meeting FV recommendations had significantly …

The Role Of Elongin B In Drosophila Melanogaster Muscle Development, Madelyne Dayan

Student Theses and Dissertations

Muscle atrophy is a result of a number of muscle diseases, as well as aging, disuse, and infections. Muscles weakened in these ways exhibit a characteristic decrease in size, strength, and mobility. The similarities between muscle in humans and Drosophila allow for direct comparisons to be made in vertebrates from studying muscle in Drosophila. Differential gene expression has been shown to play an important role in embryonic muscle development in Drosophila melanogaster. A previous study identified Elongin B (EloB) to be involved in somatic muscle morphogenesis in Drosophila, with mutant embryos displaying severe muscle defects, including thin, mis-attached, and missing …

A History Of Omics Discoveries Reveals The Correlates And Mechanisms Of Loading-Induced Hypertrophy In Adult Skeletal Muscle. 2024 Camps Young Investigator Award Invited Review, Toby L. Chambers, Kevin A. Murach

Health, Human Performance and Recreation Faculty Publications and Presentations

Since the early 2000s, omics approaches to study skeletal muscle hypertrophy consequent to loading (e.g., resistance exercise) have expanded dramatically. Beginning with genomics and transcriptomics, there are now omics datasets from hypertrophying skeletal muscle spanning methylomics, proteomics, and phosphoproteomics, with further integration of single cell/nucleus-specific omics, among others. The purpose of this review is to explore the history of leveraging omics to enable understanding and discovery with respect to loading-induced hypertrophy in adult skeletal muscle. We elaborate on key historical and contemporary studies and findings, highlight specific examples where omics discoveries led to a mechanistic understanding of skeletal muscle growth, …

Preparation And Characterization Of Phthalocyanine Photocatalysts Supported On Mixed Metal Oxides, Malak Alsharhan

Seton Hall University Dissertations and Theses (ETDs)

This research explores the development of hybrid photocatalysts by combining GeO₂ doping of metal oxides with phthalocyanine functionalization to enhance the degradation of Methyl Orange (MO) dye under visible light. Four metal oxide supports, TiO₂, Al₂O₃, SiO₂, and ZrO₂, were tested in their pristine form, then modified with GeO₂ and further functionalized by solution adsorption using two types of zinc phthalocyanines: F₁₆PcZn and F₆₄PcZn.

The degradation of MO was monitored using UV-Vis spectroscopy, and kinetic models were applied to evaluate the reaction rates. The results showed that the bare oxides exhibited little to no photocatalytic performance. GeO₂ doping …

Know Thyself: A History Of Ancient Neuroscience, Alex Delano

Departmental Honors & Graduate Capstone Projects

Neuroscience is often described as one of the youngest bodies of science, but documented study of the brain begins as early as the Neolithic period. The ancient Mediterranean was a hub for science, mathematics, philosophy, and literature, all disciplines necessary for the study of the brain. Evidence of neurosurgical procedures like trepanation and craniotomy began in the Neolithic period and were practiced and improved upon throughout the ancient Mediterranean. The first detailed studies on brain anatomy were carried out in the 3^rd century BCE in Greece which created a necessary structure for future scientists to build on. While many …

Functional Morphology Of The Primate Knee And Its Implications For Hominin Bipedal Mechanics, Catherine K. Miller

Dartmouth College Ph.D Dissertations

Bipedal locomotion is central to the human condition, having shaped our evolutionary trajectory since its early emergence in the hominin lineage. Despite its importance, key questions remain about its origins and evolutionary trajectory. While many studies have focused on locomotor-related anatomies, comparatively less attention has been paid to the knee joint – a structure critical to the primate locomotor repertoire. This dissertation aims to clarify the form-function relationship of the knee in extant primates to better infer the locomotor behaviors of Miocene hominoids and Plio-Pleistocene hominins. To address these questions, this research employs a multifaceted analytical approach, integrating linear measurements, …

Enhancing Food Safety: Detecting Pathogens In Bison, Vrinda Brahmbhatt

Honors Capstones

Bison herds are being reintroduced across Illinois for prairie conservation efforts and as an important food source, with bison meat gaining popularity as a healthier alternative to beef. However, bison can act as reservoirs of pathogens such as Shiga toxin-producing Escherichia coli (STEC) and antimicrobial resistance (AMR) genes, posing food safety and public health risks. Conservational and commercial herds are managed differently, which may influence the prevalence of STEC and associated AMR genes. To address concerns about potential AMR transmission from bison to humans via fecal-oral routes, we examined the prevalence of AMR in STEC isolates from both herd types. …

Competence Protein Comea And Oxidative Stress In Bacillus Subtilis Stationary Phase Mutagenesis, Angeline Roldan, Sam Schauf

Undergraduate Research Symposium Posters

Stationary Phase Mutagenesis is a process where a bacterium can accumulate mutations in the absence of cell division. This may occur via competent DNA uptake and oxidant DNA damage and repair. ComEA is a transmembrane protein in B. subtilis that is required for the transport of exogenous DNA into the cell. (Figure 1)

New Mexico Cooper's Hawk Demographic Study: 2024 Annual Report, Brian A. Millsap Dr.

New Mexico Cooper’s Hawk: Publications

The 2024 Annual Report for a long-term (2011 -  present) demographic study of Cooper's hawks (Astur cooperii) in Albuquerque, New Mexico.

Exploring Mobility: Phylogenetic And Structural Analysis Of The Intein In The Helicase Of Mycobacteriophage Alice, Sandra Xu

Honors Scholar Theses

This paper investigates the intein insertion in the DNA helicase gene Alice_175 of the Mycobacterium phage Alice, exploring its evolutionary significance and potential propagation mechanisms. Using phylogenetic analysis, there is evidence for gene transfer as a driving force in intein dissemination in phage and bacterial genomes. Structural predictions of the Alice_175 intein that were generated through AlphaFold2 as well as a phylogeny generated using SeaView infer a timeline of the intein's invasion and reveal key features that may influence protein functionality and host adaptation. Additionally, protein database searches suggest that intein mobility in this gene may not rely on prophages, …

Sweet Snacks Effects On Hispanic/Latino Childhood Obesity, Lemlen Belete, Brisa Rodriguez Alcantar

Undergraduate Research Symposium Lightning Talks

Background:

Obesity rates in children has become a growing concern in the Hispanic/Latino community in Nevada. This study examines the availability of sweet snacks in the home and its association with children's dietary behavior.

Methods:

Data was analyzed from a survey of 155 Latina mothers using logistic regression while controlling for income, education, and acculturation. The survey was conducted in Nevada, between October 2022 and March 2024.

Results:

The frequency of keeping sweets in the home was significantly associated with children's consumption of various sweets. For every increase in keeping sweets at home, the odds of children consuming: • Chocolate …

Developing A Trial Unique Sequencing Task For Mice, Yader Gomez, Lilly Hagos, Gage Pompa, Ariana Mitic, Orlando Montiel, Talha Soluoku

Undergraduate Research Symposium Posters

The hippocampus and the anterior cingulate cortex (ACC) play distinct yet complimentary roles in the performance of cognitively demanding tasks.

While rodent models cannot truly capture human cognition, there is a serious need to develop more cognitively demanding rodent tasks to better understand human cognition to develop new tools to fight neurodegeneration.

Sequencing tasks are some of the most difficult for rodents to perform and are notoriously difficult to train.

Cutting edge interactive touch screen technology allows us to test an endless array of sequences, in a sound-controlled enclosure featuring a simple behavioral response for the mice.

Study Aim: To …

Stress And Glucose Regulation In Adults At Risk For Type 2 Diabetes Mellitus: A Secondary Analysis From A Randomized Controlled Trial, Sophia Lysne, Regina Dispa, Brenna Way, Bea Francene Pilnade, Donya Shahamati, Romina Ballesteros, Christopher Acosta, Keren Morales, Esther Adaji, Ohis Egbaidomeh

Undergraduate Research Symposium Posters

Type 2 Diabetes Mellitus (T2DM) is a major public health concern, significantly increasing the risk of cardiovascular diseases (CVDs). Additionally, prediabetes is a critical precursor, as it elevates the risk of developing both T2DM and CVDs. (2)

Recent literature reveals among individuals with severe perceived stress, there is a higher likelihood of HbA1c% levels meeting the criteria for prediabetes (5.7– 6.4%) as compared to those with lower perceived stress. (3)

Studies show that psychological stress has hyperglycemic effects in individuals with T2DM, suggesting that stress is associated with poorer blood glucose control. (4)

Marine Heat Waves Effects On Rocky Intertidal Ecosystem Functioning, Skyler Peterson, S. Schneider, C.L. Webb, A. Leighton

Undergraduate Research Symposium Posters

Severe heat waves may cause long-term changes in marine ecosystems and are expected to increase in magnitude and frequency. Here we use surveys from six localities across a six-year interval (2013-2019) to investigate the effects of heat waves on the functional diversity of rocky intertidal ecosystems (Vancouver Island, Canada).

Functional diversity, the number and types of functions performed by organisms, influences ecosystem stability and health. To assess changes in functional diversity we used analyses of biological traits. Biological traits are measurable properties of organisms that describe their morphology, physiology, and behavior. We quantitatively assessed changes in functional diversity in response …

Genomic Characterization Of High-Grade Serous Ovarian Carcinoma Reveals Distinct Somatic Features In Black Individuals, Katherine A Lawson-Michod, Jeffrey R Marks, Lindsay J Collin, David A Nix, Natalie R Davidson, Chad D Huff, Yao Yu, Aaron Atkinson, Courtney E Johnson, Lucas A Salas, Lauren C Peres, Casey S Greene, Joellen M Schildkraut, Jennifer A Doherty

Faculty, Staff and Student Publications

Black individuals experience worse survival after a diagnosis of high-grade serous ovarian carcinoma (HGSC) than White individuals and are underrepresented in ovarian cancer research. To date, the understanding of the molecular and genomic heterogeneity of HGSC is based primarily on the evaluation of tumors from White individuals. In the present study, we performed whole-exome sequencing on HGSC samples from 211 Black patients to identify significantly mutated genes and characterize mutational signatures, assessing their distributions by gene expression subtypes. The occurrence and frequency of somatic mutations and signatures by self-reported race were compared with historic data from The Cancer Genome Atlas …

Longitudinal Profiling Of Circulating Tumor Dna Reveals The Evolutionary Dynamics Of Metastatic Prostate Cancer During Serial Therapy, Yuehui Zhao, Naveen Ramesh, Ping Xu, Emi Sei, Min Hu, Shanshan Bai, Patricia Troncoso, Ana M Aparicio, Christopher J Logothetis, Paul G Corn, Nicholas E Navin, Amado J Zurita

Faculty, Staff and Student Publications

Treatment decisions in metastatic castration-resistant prostate cancer are mostly guided by clinical variables, but efforts to molecularly monitor the disease remain hampered by challenges in acquiring tumor tissue repeatedly. In this study, we simultaneously profiled the genome copy number and exome in longitudinal plasma circulating tumor DNA (ctDNA) acquired before, during, and upon progression to serial treatments with androgen signaling inhibitors and taxane chemotherapy from 60 patients with metastatic castration-resistant prostate cancer (2-10 samples per patient). The genomic data were used to delineate the clonal substructure and evolutionary dynamics of each patient, and an evolutionary dynamic index was developed to …

Efficacy And Safety Of Larotrectinib In Patients With Trk Fusion Gastrointestinal Cancer, Changsong Qi, Lin Shen, Thierry Andre, Hyun Cheol Chung, Timothy L Cannon, Elena Garralda, Antoine Italiano, Damian T Rieke, Tianshu Liu, Domnita-Ileana Burcoveanu, Natascha Neu, Chiara E Mussi, Rui-Hua Xu, David S Hong, Alexander Drilon, Jordan Berlin

Faculty, Staff and Student Publications

Background: Larotrectinib is the first-in-class, highly selective TRK inhibitor with demonstrated efficacy in various TRK fusion solid tumours. We report the efficacy and safety of larotrectinib in patients with TRK fusion gastrointestinal (GI) cancer.

Methods: Patients with TRK fusion GI cancer from NAVIGATE (NCT02576431) were included. Response was independent review committee (IRC)-assessed per RECIST v1.1.

Results: As of July 2023, 44 patients were enrolled. Tumour types included colorectal (CRC; n = 26), pancreatic (n = 7), cholangiocarcinoma (n = 4), gastric (n = 3), and one each of appendiceal, duodenal, oesophageal and hepatic cancers. Of the 26 patients …