Life Sciences Commons^™

Enzyme Characterization Of Sabath Family Members, Troy Burtchett

Honors Theses

Enzymes can have many different functions within an organism. One such common function is methylation. The SABATH family of enzymes perform methylation of various organic acids. Many of the members of this family of enzymes have become specialized in the acid they methylate. For example: salicylic acid methyl transferase (SAMT) will primarily methylate salicylic acid, and benzoic acid methyl transferase (BAMT) will primarily methylate benzoic acid. By comparing the sequence of these enzymes, it has been found that a conserved region consisting of the sequence SSYSLMWLS appears in some form across all the members of the SABATH family. The 6 …

Pertumbuhan Dan Hasil Tanaman Jagung (Zea Mays L.) Toleran Herbisida Akibat Pemberian Berbagai Dosis Herbisida Kalium Glifosat, Agus Wahyudin, Ruminta, S. A. Nursaripah

Jurnal Kultivasi

Penelitian ini bertujuan untuk mengetahui pengaruh herbisida kalium glifosat terhadap pertumbuhan dan hasil tanaman Jagung toleran herbisida glifosat serta mencari dosis terendah yang dapat digunakan dalam budidaya jagung toleran herbisida. Percobaan dilaksanakan di Kebun Percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jatinangor, Kabupaten Sumedang, Jawa Barat dengan ketinggian tempat 750 meter di atas permukaan laut dan ordo tanah Inceptisols, curah hujan rata-rata termasuk tipe C3 menurut Oldeman, dan temperatur udara berkisar antara 22,23° - 26,47°C. Percobaan dilakukan dari bulan Desember 2014 sampai April 2015. Metode percobaan yang digunakan adalah Rancangan Acak Kelompok yang terdiri dari 8 perlakuan dan diulang sebanyak tiga …

Chronic Toxicity Of Binary‐Metal Mixtures Of Cadmium And Zinc To Daphnia Magna, Edgar Pérez, Tham C. Hoang

School of Environmental Sustainability: Faculty Publications and Other Works

The present study characterized the chronic effect of binary‐metal mixtures of cadmium (Cd) and zinc (Zn) on Daphnia magna. The titration design was chosen to characterize the 21‐d chronic effects of the binary‐metal mixtures on survival, growth, reproduction, and metal accumulation in D. magna. Using this design, increasing concentrations of Zn (10, 20, 40, 80, 120, 160, and 200 μg/L) were titrated against a constant concentration of 1.5 μg/L Cd. The results demonstrated that Cd was highly toxic to D. magna. In a mixture with Cd and Zn, sublethal concentrations of 10 and 20 μg/L Zn were …

Urban Versus Rural Differences In Insurance Coverage And Impact On Employment Among Families Caring For A Child With Cerebral Palsy, Braydon Schaible, Gavin T. Colquitt, Li Li, Manuela C. Caciula, Noelle G. Moreau

Health Sciences & Kinesiology: Faculty Publications

Background: The purpose of this study was to examine urban vs. rural differences on the relationship between family contextual variables and adequacy of insurance coverage and impact on employment for among families with a child with Cerebral Palsy from a nationally representative sample.

Methods: A retrospective, observational study was carried out using data from the National Survey of Children with Special Healthcare Needs.

Results: A total of 744 participants reported as having a child with a diagnosis of Cerebral Palsy and were included in the sample. Logistic regression analyses, adjusting for urban and rural setting revealed different predictors of adequacy …

Proceedings Of The 129th Annual Meeting Of The Iowa Academy Of Science [Abstracts & Program, 2017], Iowa Academy Of Science.

Annual Meeting of the Iowa Academy of Science [Programs & Abstracts]

Contains information about the schedule and presentations for the 2017 annual meeting of the Iowa Academy of Science, including abstracts.

Determining The Effects Of Sulfur Fertility Levels On Edamame Soybean [ Glycine Max ( L. ) Merrill ] Protein Components, Theoneste Nzaranyimana

Theses and Dissertations

Edamame [Glycine max (L.) Merr.] a vegetable type soybean is harvested and consumed before reaching its maturity stage (R6). Its health properties drew attention to U.S. consumers because of its high protein content. Proteins are the major structural component of all cells in the living organisms. They are made of essential and non-essential amino acids which play a big role in growth and development and are source of energy. Proteins act as enzymes, antibodies, and hormones and blood protein. Sulfur (S) is a component of the amino acids cysteine (Cys) and methionine (Met) which are the building blocks for proteins. …

Transcriptomic Analysis Of Maternally Provisioned Cues For Phenotypic Plasticity In The Annual Killifish, Austrofundulus Limnaeus, Amie L. Romney, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Background: Genotype and environment can interact during development to produce novel adaptive traits that support life in extreme conditions. The development of the annual killifsh Austrofundulus limnaeus is unique among vertebrates because the embryos have distinct cell movements that separate epiboly from axis formation during early development, can enter into a state of metabolic dormancy known as diapause and can survive extreme environmental conditions. The ability to enter into diapause can be maternally programmed, with young females producing embryos that do not enter into diapause. Alternately, embryos can be programmed to “escape” from diapause and develop directly by both maternal …

Asthma-Related Hospitalization And Emergency Room Visits In Oakland, Alondra Varela

McNair Research Journal SJSU

The City of Oakland has seen diminished childhood asthma rates. During the last few years more children have been able to qualify for Medi-Cal and other health insurance plans under the Affordable Care Act (ACA). Local programs work together with Medi-Cal and ACA to reduce the number of symptoms related to asthma by making sure children from low-income communities have access to primary care, medicine, and treatment. Unfortunately, the asthma-related hospitalizations and emergency room visits among children have continued to increase. These rates are even higher in low-income communities located near highly trafficked areas. To investigate this phenomenon, this study …

Hiv-1 Protease Inhibitors From Marine Brown Alga: A Literature Review, Eric Nunez Aguilar

McNair Research Journal SJSU

The human immunodeficiency virus type 1 (HIV-1) causes an infectious disease that if left untreated can progress to acquired immunodeficiency syndrome (AIDS) and be fatal. Finding a cure and more treatments for HIV has become a top priority in medical research, and due to the cost of synthetic HIV medication, finding a low-cost alternative is essential. Marine pharmacology has provided a possible solution to costly HIV medication through compounds derived from marine brown algae that inhibit the HIV-1 protease (PR). The objective of this study is to emphasize the necessity for further research in HIV-1 protease inhibition using marine wildlife-derived …

The Inhibitory Effects Demonstrated By Manuka Honey On Biofilms: How Manuka Honey May Soon Replace Conventional Antibiotic Therapy, Laura Pavelik, Cundell, Phd

CSHLA Scholar Day

Introduction:

The surge of antibiotic-resistant bacteria has raised substantial concerns over how to effectively and efficiently control antibiotic-resistant bacteria capable of secreting biofilms. A biofilm can be defined as a self-producing extracellular matrix (Carter, et, al., 2016). Manuka honey, an alternative to conventional antibiotics, has proven successful in inhibiting planktonic cells and killing bacteria living under the protection of biofilms (Brudzynski & Sjaarda, 2015)

2017 - The Twenty-Second Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Twenty-second Annual Symposium of Student Scholars, held on April 20, 2017. Includes abstracts from the presentations and posters.

Molecular And Biochemical Technologies For Modifying Plant Cell Walls And Producing Functional Biobased Products, Ningning Zhang

Student Theses and Dissertations

There is a continuing need for new molecular and biochemical processing technologies to more sustainably utilize biomass such as sugar beet pulp for environmental and economic benefit. This research project addressed the overarching research hypothesis that innovative molecular and biochemical technologies can enable more efficient processing of plant biomass and generate renewable biobased products for food and industrial use. Specifically, this research investigated upstream processing technologies toward re-engineering the plant cell wall for improved biomass processability and downstream processing technologies for efficient cell wall-depolymerizing (CWD) enzyme production and biomass processing. There were two Specific Aims addressed in this project: 1.) …

Water Quality Characteristics Of Reservoir Tailwater Recovery Systems Associated With Agriculture Production In Northeast Arkansas, Leevi Trull Haas

Student Theses and Dissertations

Reservoir tailwater recovery systems (R-TWRS) have helped shift considerations for a broader array of water storage and irrigation techniques for conventional crop production in the Mississippi River basin under conditions of declining recharge and developing cones of depression in the Mississippi Alluvial Aquifer (alluvial aquifer). Sustaining groundwater supplies further emphasizes potential water quality changes that can occur within and downstream of production sites. This study examined water quality characteristics throughout three reservoir tailwater recovery systems (R-TWRS) during events of water movement from irrigation, significant precipitation, and drawdown. While orthophosphate, dissolved nitrate, and nitrite concentrations were significantly different among R-TWRS and …

Genome Sequence Of Staphylococcus Saprophyticus Dpc5671, A Strain Isolated From Cheddar Cheese, Caitríona M. Guinane, Et. Al.

Department of Biological Sciences Publications

The draft genome sequence of Staphylococcus saprophyticus DPC5671, isolated from cheddar cheese, was determined. S. saprophyticus is a common Gram-positive bacterium detected on the surface of smear-ripened cheese and other fermented foods.

GENOME ANNOUNCEMENT

Draft Nuclear Genome Sequence Of The Liquid Hydrocarbon–Accumulating Green Microalga Botryococcus Braunii Race B (Showa), Daniel R. Browne, Jerry Jenkins, Jeremy Schmutz, Shengqiang Shu, Kerrie Barry, Jane Grimwood, Jennifer Chiniquy, Aditi Sharma, Thomas Daniel Niehaus, Taylor L. Weiss, Andrew T. Koppisch, David T. Fox, Suraj Dhungana, Shigeru Okada, Joe Chappell, Timothy P. Devarenne

Plant and Soil Sciences Faculty Publications

Botryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels. This draft genome for the B race of B. braunii will allow researchers to unravel important hydrocarbon biosynthetic pathways and identify possible regulatory networks controlling this unusual metabolism.

Non-Celiac Gluten Sensitivity Is An Emerging Spectrum Of Gluten Intolerance, Darla O'Dwyer

Symposium on Arts and Research

People following gluten free diets have become increasingly prevalent. Many people indicate that they feel better on a gluten free diet and that their symptoms return after eating gluten. This is occurring in the absence of celiac disease or a wheat allergy. This new clinical entity is called non-celiac gluten sensitivity (NCGS). Volta and colleagues (2014) cite several reasons for an increase in this condition: 1) farming practices have led to an increase in specific wheat variants that contain high amounts of gluten peptides that maybe toxic to susceptible individuals; 2) dough fermentation has significantly been shortened compared to the …

Design Of A Robust Undergraduate Biochemistry Laboratory Course Based On A Modified And Expanded Bovine Serum Albumin Purification Scheme, Odutayo O. Odunuge, Nicholas Cheatwood, John Mullins, Michele Harris

Symposium on Arts and Research

The ASBMB curriculum for an undergraduate degree recommends a set of skills that can be acquired only through laboratory courses and research experience. Based on a previously reported purification scheme for BSA (Odunuga and Shazhko, 2013), we designed a robust, reproducible, cost-effective, safe and enquiry-based undergraduate biochemistry laboratory course that encompasses many of the skill-sets recommend-ed in the ASBMB curriculum. Our work not only modi-fied certain steps in the scheme, it also included addi-tional steps to enhance student learning and skill ac-quisition. Salt precipitation, ion exchange and size ex-clusion chromatography were employed by students to purify BSA from cow plasma. …

Accuracy Assessment On Drone Measured Heights At Different Height Levels, David L. Kulhavy, I-Kuai Hung, Daniel Unger, Yanli Zhang

Symposium on Arts and Research

The advancement in unmanned aerial system (UAS) technology has made it possible to attain an aerial unit, commonly known as a drone, at an affordable price with increasing precision and accuracy in positioning and photographing. While aerial photography is the most common use of a drone, many of the models available in the market are also capable of measuring height, the height of the drone above ground, or the altitude above the mean sea level. On board a drone, a barometer is used to control the flight height by detecting the atmospheric pressure change; while a GPS receiver is mainly …

Estimating Autoantibody Signatures To Detect Autoimmune Disease Patient Subsets, Zhenke Wu, Livia Casciola-Rosen, Ami A. Shah, Antony Rosen, Scott L. Zeger

Johns Hopkins University, Dept. of Biostatistics Working Papers

Autoimmune diseases are characterized by highly specific immune responses against molecules in self-tissues. Different autoimmune diseases are characterized by distinct immune responses, making autoantibodies useful for diagnosis and prediction. In many diseases, the targets of autoantibodies are incompletely defined. Although the technologies for autoantibody discovery have advanced dramatically over the past decade, each of these techniques generates hundreds of possibilities, which are onerous and expensive to validate. We set out to establish a method to greatly simplify autoantibody discovery, using a pre-filtering step to define subgroups with similar specificities based on migration of labeled, immunoprecipitated proteins on sodium dodecyl sulfate …

Network Exploration Of Correlated Multivariate Protein Data For Alzheimer's Disease Association, Matthew J. Lane

Theses

Alzheimer Disease (AD) is difficult to diagnose by using genetic testing or other traditional methods. Unlike diseases with simple genetic risk components, there exists no single marker determining as to whether someone will develop AD. Furthermore, AD is highly heterogeneous and different subgroups of individuals develop the disease due to differing factors. Traditional diagnostic methods using perceivable cognitive deficiencies are often too little too late due to the brain having suffered damage from decades of disease progression. In order to observe AD at early stages prior to the observation of cognitive deficiencies, biomarkers with greater accuracy are required. By using …

Determining The Effects Of Palmitoylation On Rho Family Gtpase Tcl Gtp Binding Activity Using Palmitoyl Co-A, Grace Young

Honors Capstones

Capstone submitted as a graduation requirement for BSU Honors Program.

Relative Importance Of Black Carbon, Brown Carbon, And Absorption Enhancement From Clear Coatings In Biomass Burning Emissions, Rudra P. Pokhrel, Eric R. Beamesderfer, Nick L. Wagner, Justin M. Langridge, Daniel A. Lack, Thilina Jayarathne, Elizabeth A. Stone, Chelsea E. Stockwell, Robert J. Yokelson, Shane M. Murphy

Chemistry and Biochemistry Faculty Publications

A wide range of globally significant biomass fuels were burned during the fourth Fire Lab at Missoula Experiment (FLAME-4). A multi-channel photoacoustic absorption spectrometer (PAS) measured dry absorption at 405, 532, and 660 nm and thermally denuded (250 °C) absorption at 405 and 660 nm. Absorption coefficients were broken into contributions from black carbon (BC), brown carbon (BrC), and lensing following three different methodologies, with one extreme being a method that assumes the thermal denuder effectively removes organics and the other extreme being a method based on the assumption that black carbon (BC) has an Ångström exponent of unity. The …

Characterization Of A Putative Ccm Transporter, Cia8, In Chlamydomonas Reinhardtii Using Rnai Knockdown Mutants, Joshua Schwartzenburg

Honors Capstones

No abstract provided.

Nutritional Effects On Age-Related Changes In T-Cell Differentiation And Function, Michelle Chen

Whittier Scholars Program

T-cells are a type of white blood cell that in our bodies, scanning for cellular abnormalities and infections, thus making them essential for human immunity. However, due to the effects of aging, our bodies diminish the production of T cells and as a result, elderly individuals do not respond to immune disturbance as robustly as young individuals. In this experiment, I am examining the Jurkat T-cell, a human T lymphocyte cell line, viability at different treatment concentrations of the natural compounds, resveratrol and curcumin, and two cancer treatment drugs, cisplatin and bleomycin. These natural compounds, resveratrol and curcumin, have been …

Rose, Midge (Fa 1013), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid only for Folklife Archives Project 1013. Student folk studies project titled: “Hog Killing,” which includes survey sheets with brief descriptions of the hog killing process in Boyle County, Kentucky. Sheets may include a brief description of belief, traditional practice, or tool, informant’s name and photo.

Gill, Ted (Fa 1015), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid only for Folklife Archives Project 1015. Folk studies student project titled: “Bee Keeping,” which includes interview and survey sheets with brief descriptions of the beekeeping culture in Jackson County, Indiana. Sheets may include a brief description of belief or item, and a photo of bee culture.

Skees, Thomas W. (Fa 1012), Manuscripts & Folklife Archives

Folklife Archives Finding Aids

Finding aid only for Folklife Archives Project 1012. Folk studies student project titled: “Early Farming Methods and Traditional Tools” which includes survey sheets with brief descriptions of early farming beliefs and tools in Hardin County, Kentucky. Sheets may include a brief description of belief, traditional practice, or tool, informant’s name, and motif index number.

Implementation Of A Biology Summer Bridge Program For First-Year Biology Majors, Madelyn Aittama

Honors Theses

Students are entering higher education at an escalated rate, but many are unprepared for the rigorous coursework that lies ahead of them. As nationwide programs are continuously being implemented throughout high schools to better prepare students for college life, many students are still not equipped with the skills necessary to succeed in obtaining a bachelor’s degree. Many students feel discouraged and inadequate when they perform below their academic expectations, and for a large portion of students pursuing higher education, this results in discontinuing their education. While retention rates amongst STEM (science, technology, engineering and mathematics) students are particularly low, the …

Influence Of Chinese Tallow Infestation On Winter Bird Community Along Lanana Creek Basin, Kelli Bashaw, Christopher E. Comer

Undergraduate Research Conference

Exotic invasive plant species are among the greatest threats to biodiversity and persistence of rare wildlife species in the United States. These plants compete with native plants, causing extinctions or severe reductions in native populations. They may also affect wildlife through loss of preferred food items or changes in vegetation structure. Chinese tallow tree (Triadeca sebifera) is one of the most important and widespread exotic plants in the Southeast. Although impacts of Chinese tallow on vegetation communities are well studied, impacts on native wildlife are less well understood. This study explored the interaction between native wildlife and Chinese tallow infestation …

The Bees Of A.L. Mangham Jr. Regional Airport, Nacogdoches, Texas, Ryan J. Pingedot, Daniel J. Bennett

Undergraduate Research Conference

The United States is home to about 4,000 species of native bees, and many are critically important due to the pollination services they provide (Buchman & Nabhan 1996). Most of these are inconspicuous, solitary bees that nest in the ground. In recent years, a number of bee species have been shown to be in decline (Burkle 2013). Due to these declines, it is important to study and provide habitat for local bee populations.

As a result of periodic mowing, the surroundings of the A.L. Mangham Jr. Regional Airport in Nacogdoches County, Texas provide a grassy, prairie-like habitat that results in …