Life Sciences Commons^™

Geospatial Analysis Of Tursiops Truncatus, Common Bottlenose Dolphin, From Noaa Cetacean Stranding Data In Florida To Explore Human Fishery Impacts, Jamie S. Ahn

HCNSO Student Capstones

Cetacean strandings provide valuable biological and geographic information, including various human impacts to vulnerable populations. This study utilizes global information systems (GIS) to conduct geospatial analyses of common bottlenose dolphin, Tursiops truncatus, strandings impacted by fishery interactions in Florida from 2002 to 2014 along with summary statistics of the types of interaction and gear involved. In addition four human impact factors, average human population, average number of boat licenses, average number of fishing permits, and coastline length, were compared to the stranding densities per county for association and predictability. Four regions of stranding “hotspots” were identified, and all human impact …

Cloning And Expression Of Pseudomonas Aeruginosa Elastase For Elucidating The Pathophysiological Effect On Lung Tissue, Derek T. Draper

Biology Theses

Pseudomonas aeruginosa causes acute nosocomial as well as community acquired pneumonia. In cystic fibrosis patients, this organism causes recurrent infections and inflammation with a high rate of morbidity and mortality. To support research in elucidating the molecular mechanisms of Pseudomonas elastase (PE) on disease progression, PE was expressed in E. coli and the protein purified for further application. This study represents a valid experimental protocol to produce. Genomic DNA was isolated from P. aeruginosa and selected regions were amplified using PCR with lasB gene specification. Amplified DNA was ligated into plasmids and E. coli were transformed to support the …

Mallampati Scoring And Its Relation To Obstructive Sleep Apnea, Austin Korczak

Honors Capstones

According to the American Sleep Apnea Association potentially 80% of moderate to severe cases of Obstructive Sleep Apnea (OSA) go undiagnosed with even optimistic estimates stating that at least 22 million Americans are afflicted by the disorder. These numbers are expected to continue to increase due to the rising rates of obesity in America. As a result, reliable criteria that can help physicians accurately predict the presence of an underlying sleep disorder, whose symptoms are masked during the day, is highly sought after. The objective of this research is to begin defining these criteria via a literature review of articles …

Neural Expression And Function Of Autism Candidate Genes In Zebrafish, Elyse P. Kite

Biology Theses

The Autism Spectrum Disorders (ASD) refer to a large heterogeneous set of developmental disorders defined by two behavioral domains: 1) Impairment of social comprehension and abnormal development of social communication, and 2) the presence of stereotypical and repetitive behaviors. The etiology of ASD is complex, with both genetic and environmental components. De novo and inherited mutations within DPP6 and PSMD12 have been identified within individuals diagnosed with ASD suggesting an etiologic role. Understanding the functionality of these genes could lead novel treatments for ASD. Zebrafish were utilized as a model system for the assessment of functionality of the orthologous genes …

Mapping The Spatial-Temporal Variation In Ras Ghanada Seagrass Meadows And Sand Shoals Between 1996, 2006 & 2012, Ryan Brookbank

HCNSO Student Theses and Dissertations

Seagrass meadows offshore Ras Ghanada, as elsewhere, are an important component to the ecosystem providing numerous benefits to both aquatic and human life. This work focused on mapping the spatial and temporal distribution of seagrass meadows offshore Ras Ghanada using aerial photography acquired in 1996 and high-resolution satellite images captured in 2006 and 2012. The movements of sand shoals were also tracked, so as to further explain the dynamics of this ecosystem, as it is the area between the shoal crests that hosts the best developed seagrass meadows. The natural limiting factor for seagrass on the Ras Ghanada coastal shelf …

Evaluation Of Modis Land Surface Temperature Data To Estimate Near-Surface Air Temperature In Northeast China, Yuan Z. Yang, Wen H. Cai, Jian Yang

Forestry and Natural Resources Faculty Publications

Air temperature (T_air) near the ground surface is a fundamental descriptor of terrestrial environment conditions and one of the most widely used climatic variables in global change studies. The main objective of this study was to explore the possibility of retrieving high-resolution T_air from the Moderate Resolution Imaging Spectroradiometer (MODIS) land surface temperature (LST) products, covering complex terrain in Northeast China. The All Subsets Regression (ASR) method was adopted to select the predictors and build optimal multiple linear regression models for estimating maximum (T_max), minimum (T_min), and mean (T_mean) air temperatures. …

Reducing The Metabolic Cost Of Walking With An Ankle Exoskeleton: Interaction Between Actuation Timing And Power, Samuel Galle, Philippe Malcolm, Steven Hartley Collins, Dirk De Clercq

Journal Articles

Background

Powered ankle-foot exoskeletons can reduce the metabolic cost of human walking to below normal levels, but optimal assistance properties remain unclear. The purpose of this study was to test the effects of different assistance timing and power characteristics in an experiment with a tethered ankle-foot exoskeleton.

Methods

Ten healthy female subjects walked on a treadmill with bilateral ankle-foot exoskeletons in 10 different assistance conditions. Artificial pneumatic muscles assisted plantarflexion during ankle push-off using one of four actuation onset timings (36, 42, 48 and 54% of the stride) and three power levels (average positive exoskeleton power over a stride, summed …

A Longitudinal Cline Characterizes The Genetic Structure Of Human Populations In The Tibetan Plateau, Choongwon Jeong, Benjamin M. Peter, Buddha Basnyat, Maniraj Neupane, Geoff Childs, Sienna Craig, John Novembre, Anna Di Rienzo

Dartmouth Scholarship

Indigenous populations of the Tibetan plateau have attracted much attention for their good performance at extreme high altitude. Most genetic studies of Tibetan adaptations have used genetic variation data at the genome scale, while genetic inferences about their de- mography and population structure are largely based on uniparental markers. To provide genome-wide information on population structure, we analyzed new and published data of 338 individuals from indigenous populations across the plateau in conjunction with world- wide genetic variation data. We found a clear signal of genetic stratification across the east- west axis within Tibetan samples. Samples from more eastern locations …

Investigating Agricultural Management Practices And Environmental Sustainability In Jordan, Mohammed I. Al-Qinna Dr., Mohammed Ali Salahat Dr.

Journal of Environmental Sustainability

Jordan, as other developing countries in the world, is struggled by instable agricultural development due to existing various water pressures (e.g. increase in irrigation demands, deterioration of water quality, industrial pollution, water borne sewerage, etc.), climate change impacts, and biophysical and socioeconomic barriers (e.g. rapid population growth, social unsettlements, sudden refuges and migrations, limited economic resources, low levels of technology, environmental limitations and stresses, and widespread poverty). One of the adaptive key issues for the Jordanian environmental sustainable development is the awareness of farmers on the water savings and environmental protections at farm level. The aim of this study was …

Developments And Properties Of Plastic Mimicking Biopolymers For Food Packaging Application, Daniel I. Hefft

Journal of Applied Packaging Research

Sustainability takes an ever increasing importance in the food industry. Here not only the selection of raw materials plays a role, but also packaging materials. Nowadays there is an increasing number of biodegradable and bio-compostable packaging materials available.

However, these materials are still not able to replace mineral oil based packaging materials completely, nevertheless they are becoming increasingly important, since there must be solutions to problems such as pollution of the oceans by plastic waste, growing piles of rubbish from growing population, etc.

The following literature review shows briefly recent developments on plastic mimicking biopolymers, as these materials guarantee great …

Bk Channels Mediate Synaptic Plasticity Underlying Habituation In Rats, Tariq Zaman, Cleusa De Oliveira, Mahabba Smoka, Chakravarthi Narla, Michael O. Poulter, Susanne Schmid

Anatomy and Cell Biology Publications

Habituation is a basic form of implicit learning and represents a sensory filter that is disrupted in autism, schizophrenia, and several other mental disorders. Despite extensive research in the past decades on habituation of startle and other escape responses, the underlying neural mechanisms are still not fully understood. There is evidence from previous studies indicating that BK channels might play a critical role in habituation. We here used a wide array of approaches to test this hypothesis. We show that BK channel activation and subsequent phosphorylation of these channels are essential for synaptic depression presumably underlying startle habituation in rats, …

Sf3b1 Is A Stress-Sensitive Splicing Factor That Regulates Both Hsf1 Concentration And Activity, Karen S. Kim Guisbert, Eric Guisbert

Biomedical Engineering and Sciences Faculty Publications

The heat shock response (HSR) is a well-conserved, cytoprotective stress response that activates the HSF1 transcription factor. During severe stress, cells inhibit mRNA splicing which also serves a cytoprotective function via inhibition of gene expression. Despite their functional interconnectedness, there have not been any previous reports of crosstalk between these two pathways. In a genetic screen, we identified SF3B1, a core component of the U2 snRNP subunit of the spliceosome, as a regulator of the heat shock response in Caenorhabditis elegans. Here, we show that this regulatory connection is conserved in cultured human cells and that there are at least …

Activity Of Distinct Growth Factor Receptor Network Components In Breast Tumors Uncovers Two Biologically Relevant Subtypes, Moom Roosan, Shelley M. Macneil, David F. Jenkins, Gajendra Shrestha, Sydney R. Wyatt, Jasmine A. Mcquerry, Stephen R. Piccolo, Laura M. Heiser, Joe W. Gray, W. Evan Johnson, Andrea H. Bild

Pharmacy Faculty Articles and Research

Background
The growth factor receptor network (GFRN) plays a significant role in driving key oncogenic processes. However, assessment of global GFRN activity is challenging due to complex crosstalk among GFRN components, or pathways, and the inability to study complex signaling networks in patient tumors. Here, pathway-specific genomic signatures were used to interrogate GFRN activity in breast tumors and the consequent phenotypic impact of GRFN activity patterns.

Methods
Novel pathway signatures were generated in human primary mammary epithelial cells by overexpressing key genes from GFRN pathways (HER2, IGF1R, AKT1, EGFR, KRAS (G12V), RAF1, BAD). The pathway analysis toolkit Adaptive Signature Selection …

Activity Of Distinct Growth Factor Receptor Network Components In Breast Tumors Uncovers Two Biologically Relevant Subtypes, Mumtahena Rahman, Shelley M. Macneil, David F. Jenkins, Gajendra Shrestha, Sydney R. Wyatt, Jasmine A. Mcquerry, Stephen R. Piccolo, Laura M. Heiser, Joe W. Gray, W. Evan Johnson, Andrea H. Bild

Faculty Publications

Background: The growth factor receptor network (GFRN) plays a significant role in driving key oncogenic processes. However, assessment of global GFRN activity is challenging due to complex crosstalk among GFRN components, or pathways, and the inability to study complex signaling networks in patient tumors. Here, pathway-specific genomic signatures were used to interrogate GFRN activity in breast tumors and the consequent phenotypic impact of GRFN activity patterns.

Methods: Novel pathway signatures were generated in human primary mammary epithelial cells by overexpressing key genes from GFRN pathways (HER2, IGF1R, AKT1, EGFR, KRAS (G12V), RAF1, BAD). The pathway analysis toolkit Adaptive Signature …

Think With Your Fork: Five Areas Of Intervention For Kimball Dining Hall, Kristin Lane, Christina Nee, Julia Metzger

Philosophy Department Student Scholarship

Our goal with the present proposal is to bring modifications to the dining experience in Kimball Hall at the College of the Holy Cross, so to encourage a more well-rounded approach, which would best represent the values of continuous learning and education of the whole person and reflect the mission of the College. In order to meet these goals, we recommend five changes to Kimball dining , which we plan to implement during the Spring 2017 semester in collaboration with two Montserrat seminars.

Qt Prolongation Is Associated With Increased Mortality In End Stage Liver Disease, Sun Moon Kim, Bennet George, Diego Alcivar-Franco, Charles L. Campbell, Richard Charnigo, Brian P. Delisle, Jonathan Hundley, Yousef Darrat, Gustavo Morales, Samy-Claude Elayi, Alison L. Bailey

Internal Medicine Faculty Publications

AIM

To determine the prevalence of QT prolongation in a large series of end stage liver disease (ESLD) patients and its association to clinical variables and mortality.

METHODS

The QT interval was measured and corrected for heart rate for each patient, with a prolonged QT cutoff defined as QT > 450 ms for males and QT > 470 ms for females. Multiple clinical variables were evaluated including sex, age, serum sodium, international normalized ratio, creatinine, total bilirubin, beta-blocker use, Model for End-Stage Liver Disease (MELD), MELD-Na, and etiology of liver disease.

RESULTS

Among 406 ESLD patients analyzed, 207 (51.0%) had QT prolongation. …

Genome-Wide Analysis Of Atp-Binding Cassette (Abc) Transporters In The Sweetpotato Whitefly, Bemisia Tabaci, Lixia Tian, Tianxue Song, Rongjun He, Yang Zeng, Wen Xie, Qingjun Wu, Shaoli Wang, Xuguo Zhou, Youjun Zhang

Entomology Faculty Publications

Background: ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants.

Results: A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 …

Nutrient Loading Reduction In A Tile Drained Agricultural Watershed Through Watershed-Scale Cover Cropping: A High Resolution Analysis, Benjamin Gerald Bruening

Theses and Dissertations

Nutrient pollution originating from agricultural regions in the Midwest is a serious issue, leading to pollution of drinking water sources as well as large hypoxic zones in the Gulf of Mexico. The source of much of this contamination has been shown to be runoff from agricultural fields in the Upper Mississippi River Basin. One method that has been shown to reduce this pollution from the Upper Mississippi River Basin is the planting of winter cover crops. Winter cover crops such as rye and tillage radish have been shown to significantly reduce nitrate exported from agricultural fields, even in tile-drained watersheds …

The Role Of Tributaries In Structuring Mussel Communities, Cassie M. Vaughan

Biology Theses

Mussel species composition in tributaries compared to the river mainstem, and the influence of tributaries on the river mainstem of mussel communities, are poorly understood. This paper aims to understand differences in mussel habitat associations between tributaries and the Neches River mainstem and if the mussel communities are nested in tributaries. During the months June through October in 2016, 28 tributaries and 22 mainstem sites were surveyed in the Neches River using tactile time search. A total of 3,620 individuals were collected, of which 44.5% were collected from tributaries. However, only eight of the 28 tributaries contained mussels, while every …

Growth Of Orbicella Faveolata In La Parguera, Puerto Rico, Darren B. Marshall

HCNSO Student Theses and Dissertations

Reef-building corals are subject to high amounts of stress, including pollution and rising sea surface temperatures due to climate change. These factors can affect the ability of corals to produce their calcium carbonate skeletons. Evaluation of the effects of climate change may be facilitated by evaluation of records of coral skeletal growth over a long period of time. The aim of this study was to evaluate skeletal growth of the coral Orbicella faveolata in La Parguera, Puerto Rico over a 32-year period. For this, 14 Orbicella faveolata core samples were collected from corals at two reefs (1.2 km apart) in …

Salting Our Freshwater Lakes, Hilary A. Dugan, Sarah L. Bartlett, Samantha M. Burke, Jonathan P. Doubek, Flora Krivak-Tetley

Dartmouth Scholarship

The highest densities of lakes on Earth are in north temperate ecosystems, where increasing urbanization and associated chloride runoff can salinize freshwaters and threaten lake water quality and the many ecosystem services lakes provide. However, the extent to which lake salinity may be changing at broad spatial scales remains unknown, leading us to first identify spatial patterns and then investigate the drivers of these patterns. Significant decadal trends in lake salinization were identified using a dataset of long-term chloride concentrations from 371 North American lakes. Landscape and climate metrics calculated for each site demonstrated that impervious land cover was a …

Non-Capsular Virulence Factors Of Cryptococcus Neoformans, Kirk Nickish

Senior Honors Theses

Cryptococcus neoformans is an emerging pathogen that kills hundreds of thousands every year, especially in underdeveloped areas with little access to modern medical care. New treatments for the disease are needed to shorten treatment and decrease the side effects and costs associated with the drugs currently in use. Many C. neoformans genes have been identified that are necessary for full virulence in the host. Cir1, a regulatory protein associated with iron regulation, and Zip1, a surface zinc transporter, are both necessary for full virulence in the host. Anti-fungals targeted at these proteins or the proteins produced by other genes discussed …

Mechanical Harvesting Effectively Controls Young Typha Spp. Invasion And Unmanned Aerial Vehicle Data Enhances Post-Treatment Monitoring, Shane C. Lishawa, Brendan D. Carson, Jodi S. Brandt, Jason M. Tallant, Nicholas Reo, Dennis Albert, Andrew Monks, Joseph Lautenbach, Eric Clark

Dartmouth Scholarship

The ecological impacts of invasive plants increase dramatically with time since invasion. Targeting young populations for treatment is therefore an economically and ecologically effective management approach, especially when linked to post-treatment monitoring to evaluate the efficacy of management. However, collecting detailed field-based post-treatment data is prohibitively expensive, typically resulting in inadequate documentation of the ecological effects of invasive plant management.

Alternative approaches, such as remote detection with unmanned aerial vehicles (UAV), provide an opportunity to advance the science and practice of restoration ecology. In this study, we sought to determine the plant community response to different mechanical removal treatments to …

Accumulation And Expression Of Multiple Antibiotic Resistance Genes In Arcobacter Cryaerophilus That Thrives In Sewage, Jess A. Millar, Rahul Raghavan

Biology Faculty Publications and Presentations

We explored the bacterial diversity of untreated sewage influent samples of a wastewater treatment plant in Tucson, AZ and discovered that Arcobacter cryaerophilus, an emerging human pathogen of animal origin, was the most dominant bacterium. The other highly prevalent bacteria were members of the phyla Bacteroidetes and Firmicutes, which are major constituents of human gut microbiome, indicating that bacteria of human and animal origin intermingle in sewage. By assembling a near-complete genome of A. cryaerophilus, we show that the bacterium has accumulated a large number of antibiotic resistance genes (ARGs) probably enabling it to thrive in the wastewater. We also …

Persistent Neurobehavioral Traits In A Mouse Model Of Prenatal Ethanol Exposure, Jill M. Lawrence

Neuroscience Honors Papers

Fetal Alcohol Spectrum Disorders (FASD) effect an estimated 2% of the population, causing a range of symptoms: from craniofacial defects to inhibited cortical growth (May, et al., 2009; Murawski, et al., 2015). Impaired medial forebrain function apparent in FASD is associated with lifelong cognitive behavioral deficits, but these consequences may be avoided with early diagnosis and intervention (Streissguth, et al., 2004). Our goal is to identify early neurobehavioral abnormalities that persist into adulthood that could potentially serve as early indicators for FASD. Mouse models of prenatal ethanol exposure were developed using a voluntary drinking paradigm that introduced a sweetened ethanol …

Examination Of Neurocorrelates Of Mild Traumatic Brain Injury In Young Adults, Rachel J. Raucci

Neuroscience Honors Papers

In recent years, there has been an upswing in the number of concussion diagnoses per year in the United States, particularly in young athletes with still-developing brains. Accompanying this recent trend is an increased amount of research on concussions and their long-term impacts. This ongoing research project collects and compares data from concussed and non-concussed individuals using various neuropsychological batteries, self-report surveys and participants’ EEG readings. Data analysis of the results from 51 participants indicates that previously concussed individuals differ from their non-concussed counterparts. Specifically, individuals who have suffered a concussion exhibit specific and occasionally idiosyncratic deficits in executive control …

Assessing The Rate And Extent Of Transgenerational Acclimation And Adaptation To Ocean Warming, Abby C. Nease

HCNSO Student Theses and Dissertations

A primary goal of climate change research is to determine if species will be able to persist in a warmer environment. Most studies predict climate change will cause many species to become extinct. However, these predictions are based on experiments where only a single life stage or generation of a species was exposed to predicted future conditions (i.e. shock treatments), and thus overlook the possibility of species adapting or acclimatizing to new environmental conditions over multiple generations. As a result, current projections of species persistence through climate change are likely to overestimate species extinction. In this study, the rate and …

Functional Human Grin2b Promoter Polymorphism And Variation Of Mental Processing Speed In Older Adults, Yang Jiang, Ming Kuan Lin, Gregory A. Jicha, Xiuhua Ding, Sabrina L. Mcilwrath, David W. Fardo, Lucas S. Broster, Frederick A. Schmitt, Richard J. Kryscio, Robert H. Lipsky

Behavioral Science Faculty Publications

We investigated the role of a single nucleotide polymorphism rs3764030 (G > A) within the human GRIN2B promoter in mental processing speed in healthy, cognitively intact, older adults. In vitro DNA-binding and reporter gene assays of different allele combinations in transfected cells showed that the A allele was a gain-of-function variant associated with increasing GRIN2B mRNA levels. We tested the hypothesis that individuals with A allele will have better memory performance (i.e. faster reaction times) in older age. Twenty-eight older adults (ages 65-86) from a well-characterized longitudinal cohort were recruited and performed a modified delayed match-to-sample task. The rs3764030 polymorphism was …

Role Of Modern Immunotherapy In Gastrointestinal Malignancies: A Review Of Current Clinical Progress, Zin W. Myint, Gaurav Goel

Markey Cancer Center Faculty Publications

Gastrointestinal (GI) cancers are a group of highly aggressive malignancies with a huge disease burden worldwide. There is clearly a significant unmet need for new drugs and therapies to further improve the treatment outcomes of GI malignancies. Immunotherapy is a novel treatment strategy that is emerging as an effective and promising treatment option against several types of cancers. CTLA-4 and PD-1 are critical immune checkpoint molecules that negatively regulate T cell activation via distinct mechanisms. Immune checkpoint blockade with antibodies directed against these pathways has already shown clinical efficacy that has led to their FDA approval in the treatment of …

Health Benefits Of A Plant-Based Diet, Jenna Jackson

Honors Theses

While the rise and spread of Western culture has created remarkable advances in science, healthcare, and technology, it has also brought about a variety of chronic diseases that never once existed. Much of these ailments can be attributed to the typical Western diet, which places heavy emphasis on meats, dairy, and refined grains, contrasting the typical Eastern or Oriental diet which primarily focuses on fruits, vegetables, fish, and whole grains. This dietary transition has resulted in increased glycemic load, increased levels of unhealthy fats and cholesterol, decreased micronutrient and fiber levels, and skewed acid-base and sodium-potassium ratios. All of these …