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Articles 11131 - 11160 of 254382

Full-Text Articles in Life Sciences

The Effects Of Ataxin-1 Overexpression In The Central Nervous System On Specific Behavioral And Physiological Characteristics Of Drosophila Melanogaster, Sydney Davis May 2025

The Effects Of Ataxin-1 Overexpression In The Central Nervous System On Specific Behavioral And Physiological Characteristics Of Drosophila Melanogaster, Sydney Davis

Theses and Dissertations

Spinocerebellar ataxia type 1 (SCA1) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the ataxin-1 gene, which results in an abnormally long polyglutamine tract. Similarly, overexpression of wildtype Ataxin-1 (wt-Atx-1) in Drosophila melanogaster leads to neurodegeneration, reduced lifespan, and impaired negative geotaxis. Observations include disruptions in sleep-wake cycles, increased oxidative stress biomarkers (like protein carbonyls), and dysregulation of developmental genes such as armadillo (arm) and nemo kinase (nmo). Additionally, elevated expression of SUMOylation-related genes (Aos-1, Uba2, and dSmt3 or dSumo) and increased SUMO protein levels indicate enhanced SUMOylation in the brain. Quantitative analysis of neuronal …

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Taking Flight: Utilizing Unmanned Aerial Systems To Sample Insects In Soybean, William "Covey" Lockhart May 2025

Taking Flight: Utilizing Unmanned Aerial Systems To Sample Insects In Soybean, William "Covey" Lockhart

Theses and Dissertations

Insect scouting in soybean is a labor-intensive task but is needed throughout the season to minimize losses. When soybeans are tall, soils are wet, or soybeans are planted in narrow rows, walking through these fields can be difficult. To overcome these limitations of manual sampling, an unmanned aerial vehicle (UAV) sampling platform was developed that can collect insects in a sweep net attached to the bottom of a UAV. This system has been named the “Drone Insect Net Sampling System” (DINSS). Previous research with this system has shown positive correlations to traditional manual sampling methods. To improve this system, modifications …

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Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish, Dianna Wilson May 2025

Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish, Dianna Wilson

Theses and Dissertations

The objective of this research was to investigate the biopreservative effects of chitosan and lemon essential oil in slush ice treatments on the microbial shelf life of refrigerated catfish fillets. Catfish fillets were stored in slush ice treatments containing chitosan (CH), lemon essential oil (LO), a combination (CH+LO), water (CTRL), and no slush (NS) for three d at 4 C, then stored at 4C for twelve d post-slush. The growth of background microflora in catfish fillets treated with CH and CH+LO was reduced (P<0.05), maintaining total aerobic counts below 4.5 log CFU/g, while the control reached spoilage levels (>7 log CFU/g) by Day 6. Chitosan effectively extended the microbial shelf life but lowered pH, …

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A Description Of Mental Health Policies In Ohio Secondary Schools, Laura L. Harris, Molly Rowe May 2025

A Description Of Mental Health Policies In Ohio Secondary Schools, Laura L. Harris, Molly Rowe

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE To compare secondary school mental health policies implemented for the student body with those implemented for student athletes.

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Athletic Trainers Confidence In Evaluating And Treating Emergent Conditions, Andrea Cripps, Jenny Toonstra, Sara Stiltner May 2025

Athletic Trainers Confidence In Evaluating And Treating Emergent Conditions, Andrea Cripps, Jenny Toonstra, Sara Stiltner

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE The profession of Athletic Training (AT) has undergone a substantial educational overhaul in the last decade, adding more advanced knowledge and skills than ever before. With more than 50% of the profession having obtained their certification more than 10 years prior,1 it is essential that we understand the varying levels of confidence in evaluating and managing these new skills, so that appropriate continuing education opportunities can be developed to bridge any gaps that may be identified. The purpose of this study to examine self-reported confidence in evaluating and managing acute care skills.

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Sternocleidomastoid Syndrome In A Collegiate Basketball Athlete: A Case Study, Sara Stiltner, Rob Whitehurst, Eric Avers May 2025

Sternocleidomastoid Syndrome In A Collegiate Basketball Athlete: A Case Study, Sara Stiltner, Rob Whitehurst, Eric Avers

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

BACKGROUND
20-year-old male basketball athlete with recurring upper and lower extremity instability injuries presented fall 2021 with shoulder and ankle instability. A past medical history of thermal capsulorrhaphy from high school with surgical intervention May 2021 (college first year). October 2021 athlete began experiencing rotator cuff strains in both shoulders. November 2021, December 2021, January 2022 athlete experiences recurrent right ankle sprains. Each ankle sprain resulted in two weeks of lost participation, followed by one week of active participation. The third ankle sprain associated with increased swelling and pain over medial ankle. Upon referral and MRI a deltoid ligament sprain …

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Causes Of Athletic Trainer Attrition: A Critically Appraised Topic, Mckenna E. Tuttle, Klevione D. Brown, Elizabeth A. Starns May 2025

Causes Of Athletic Trainer Attrition: A Critically Appraised Topic, Mckenna E. Tuttle, Klevione D. Brown, Elizabeth A. Starns

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Athletic Training (AT) attrition has been a persistent issue within the profession for years1,2. By identifying the factors contributing to the attrition rates among Athletic Trainers, targeted interventions can be developed to mitigate turnover and decrease attrition within the profession.

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Kinematic Analysis Of Lower Extremity Movement Patterns During Functional Tests In Collegiate Track And Field Runners To Predict Injury Incidence, Maddie Hoyt, Brooke Parker, Bailey Weise, Andrew Busch May 2025

Kinematic Analysis Of Lower Extremity Movement Patterns During Functional Tests In Collegiate Track And Field Runners To Predict Injury Incidence, Maddie Hoyt, Brooke Parker, Bailey Weise, Andrew Busch

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
Lower extremity overuse injuries are becoming increasingly more prevalent in endurance-based running sports1,2,3,4,5,6. These injuries negatively impact an athlete’s sports performance and increase the risk of additional injuries3,4. The purpose of this study was to first examine kinematic and kinetic variables within distance runners and compare those with previous lower extremity injuries to those without reported injuries; and second to understand if such variables predicted future injuries sustained throughout a track season.

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Injury Epidemiology In Collegiate Marching Band, Morgan Davis, Jinghzen Yang, Matthew Brancaleone May 2025

Injury Epidemiology In Collegiate Marching Band, Morgan Davis, Jinghzen Yang, Matthew Brancaleone

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
Marching bands have become more popular and physically demanding, with performers taking up to 16,174 steps on game days, which may increase their risk of musculoskeletal injuries. However, research on these performers remains limited. The purpose of this study was to describe the incidence of reported injury and pain and soreness experienced by marching artists within a collegiate marching band.

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Atypical Anterior Tibial Stress Fracture Leading To Acute Fracture And Intermedullary Rodding, Emily Mulkey Swisher, Chad Cochran, Raymond Duffett May 2025

Atypical Anterior Tibial Stress Fracture Leading To Acute Fracture And Intermedullary Rodding, Emily Mulkey Swisher, Chad Cochran, Raymond Duffett

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
This case highlights an unusual stress fracture progressing to an acute tibial fracture requiring intermedullary rodding.

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The Athletic Trainer's Responsibility In Disability Exercise Adherence, Elizabeth Starns, Stephanie M. Kubiak, Rebecca J. Wehler May 2025

The Athletic Trainer's Responsibility In Disability Exercise Adherence, Elizabeth Starns, Stephanie M. Kubiak, Rebecca J. Wehler

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
This study examined exercise adherence to aerobic and strength training exercise guidelines in individuals with disabilities, highlighting the implications for athletic training (AT). Because disabilities impact over 25% of the U.S. population1, an understanding of the varied exercise adherence patterns can inform inclusive AT best practices and assist in promoting exercise and sport participation.

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Emergency Action Planning In Collegiate Athletics: An Evaluation Of The Current Adoption Of Best Practice Recommendations, Kyler Conn, Ken Blood, Genna Fusco May 2025

Emergency Action Planning In Collegiate Athletics: An Evaluation Of The Current Adoption Of Best Practice Recommendations, Kyler Conn, Ken Blood, Genna Fusco

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
The primary objective of this study was to assess the current adoption of the National Athletic Trainers’ Association’s (NATA) best practice recommendations regarding emergency planning, outlined in the NATA Position Statement: Emergency Planning in Athletics, at the collegiate level.

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Effect Of Hearing Status On Modified Balance Error Scoring System Performance, Matthew Brancaleone, Claire Akard, Jaclyn Caccese May 2025

Effect Of Hearing Status On Modified Balance Error Scoring System Performance, Matthew Brancaleone, Claire Akard, Jaclyn Caccese

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE
Concussion assessments typically include evaluations of self-reported symptoms, neurocognitive function, and postural control. Specifically, postural control is often assessed using the modified Balance Error Scoring System (mBESS). Although normative mBESS data exist, they may not be appropriate for all athletes, such as those who are deaf or hard-of-hearing (D/HoH). Given the relationship between the cochlea and the vestibular system, athletes who are D/HoH may exhibit differences in postural control due to potential vestibular dysfunction. Therefore, the objective of this study was to compare mBESS performance between athletes who are D/HoH and athletes who are hearing.

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Spontaneous Pneumothorax In A Male High School Wrestler, Laura Breese, Sara Stiltner May 2025

Spontaneous Pneumothorax In A Male High School Wrestler, Laura Breese, Sara Stiltner

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE/BACKGROUND
A pneumothorax is the presence of air in the pleural space, it is a life threatening condition and can be classified as spontaneous, acquired, or by pathophysiology. (1)(2) This specific case presents the incident of a spontaneous pneumothorax in an adolescent population.

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Engineering A Novel Treg Population To Control Autoimmune Diabetes, Sofia Colon Guzman, Elly Puckett, Mary A. Markiewicz, Ryan T. Fischer May 2025

Engineering A Novel Treg Population To Control Autoimmune Diabetes, Sofia Colon Guzman, Elly Puckett, Mary A. Markiewicz, Ryan T. Fischer

Research Days

We created a model to test the immunosuppressive capacity of Helios+FOXP3+ eTregs compared to nTregs against islet-specific autoreactive T cells. We also showed that nTregs decrease the response of these cytotoxic T cells when co-cultured with BetaLox5 cells.

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Capturing The Representational Dynamics Of Face Perception In Deep Recurrent Neural Networks, Hossein Adeli, Chase W. King, Nikolaus Kriegeskorte May 2025

Capturing The Representational Dynamics Of Face Perception In Deep Recurrent Neural Networks, Hossein Adeli, Chase W. King, Nikolaus Kriegeskorte

MODVIS Workshop

We investigate the representational dynamics of recurrent convolutional neural networks (RCNNs) in order to understand the time-course of visual recognition. We explore a family of models with bottom-up and lateral connections that were optimized for face-identification and object-recognition tasks. Using representational similarity analysis (RSA), we observed that only models that were trained for face identification showed a late-emerging prominent distinction of identities as seen in the monkey face patch AM. Early model responses strongly separated the objects from the faces. These findings suggest that the dynamics of face recognition that emerges in a hierarchical recurrent neural network prioritizes category-level recognition …

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Attention Improves Classification Performance Of Locations In Ventral And Dorsal Visual Pathways, Jinho Lee, Sung-Mu Lee, Ishita Agarwal, Anne Sereno May 2025

Attention Improves Classification Performance Of Locations In Ventral And Dorsal Visual Pathways, Jinho Lee, Sung-Mu Lee, Ishita Agarwal, Anne Sereno

MODVIS Workshop

Visual processing is divided into a ventral and dorsal pathway, thought to be important for object recognition and spatial cognition, respectively. Yet both streams encode shape and spatial information, and both are modulated by attention and task demands. This study compared how attention influences fMRI bold responses in a ventral region (fusiform gyrus, FG) and dorsal region (superior parietal gyrus, SPG). Specifically, using multivariate pattern analysis within each region, we examined whether attention modulated spatial location classification performance. During fMRI scans, participants performed one of 3 possible tasks: a 1-back task under attend-to-shape (detect a shape repetition); a 1-back task …

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Targeting The Beiging Of Muscle-Associated Adipose Tissue To Promote Muscle Regeneration, Jacob C. Parson May 2025

Targeting The Beiging Of Muscle-Associated Adipose Tissue To Promote Muscle Regeneration, Jacob C. Parson

WUSM Theses and Dissertations – All Programs

The expansion of intra-muscular adipose tissue (IMAT) between myofibers and epi-muscular adipose tissue (EMAT) outside of the muscle boundary accompanies numerous pathologies including diabetes, muscular dystrophy, and aging. IMAT and EMAT are anatomically juxtaposed to myofibers. Some evidence supports that IMAT and EMAT impede muscle function. This is potentially due to paracrine factors that are secreted by IMAT/EMAT. Different adipose depots can be classified in numerous ways including where they are located anatomically as well as the factors in which they secrete. Unlike classical white fat, adipose depots classified as brown or beige, noted by the marker uncoupling protein-1 (UCP-1) …

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Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun May 2025

Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the application of a novel thermophilic lytic polysaccharide monooxygenase (LPMO ) in the efficient cellulase-mediated hydrolysis of lignocellulosic substrates.[Method ] The thermophilic enzyme mgLPMO 10,derived from a compost metagenome,was heterologously expressed in Escherichia coli (E.coli ).Its expression level was improved by modifying the signal peptide of the expression vector and induction conditions (temperature,IPTG concentration,and induction time ).The enzyme was then characterized in terms of the optimal temperature,optimal pH,thermal stability,pH stability,and the effects of metal ions on its enzyme activity.Subsequently,the synergistic hydrolysis efficiency of mgLPMO 10 with two thermophilic endoglucanases (TnCelB and DtCelB ) …

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Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia May 2025

Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia

Journal of Food Science and Biotechnology

[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of …

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Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis, Junbao Huang, Xilin Huang, Yu Chen, Xuli Gao, Yani Luo, Wei Tao, Yongyuan Liu, Mingyu Guo, Jing Wu, Yan Liu May 2025

Phrg-Rapg-Degu Quorum Sensing Regulates Menaquinone- 7 Production In Bacillus Subtilis, Junbao Huang, Xilin Huang, Yu Chen, Xuli Gao, Yani Luo, Wei Tao, Yongyuan Liu, Mingyu Guo, Jing Wu, Yan Liu

Journal of Food Science and Biotechnology

[Objective ] The effects of the transcription regulation system on menaquinone- 7 (MK- 7) production in Bacillus subtilis (B.subtilis ) 168 were studied by excavating transcription regulators.[Method ] With the signal molecule PhrG pentapeptide,RapG,and the transcription regulator DegU,a PhrG-RapG-DegU regulatory system was successfully constructed in B.subtilis 168 to fine-tune the expression of key genes,so that the expression of key genes in the MK- 7 synthesis pathway can respond to the changes in cell density.[Result ] The production of MK- 7 in strain BS 06 reached 102.47 mg/L through this system.[Conclusion ] Fine tuning of quorum sensing can effectively improve the …

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Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg, Shengyao Wu, Zikun Ye, Weiyi Xie, Yi Wang, Haotian Ouyang, Chengcheng Zhang, Shenyan Ma May 2025

Effects Of Edible Fungal Polysaccharides On The Proliferation And Metabolism Of Lactobacillus Rhamnosus Gg, Shengyao Wu, Zikun Ye, Weiyi Xie, Yi Wang, Haotian Ouyang, Chengcheng Zhang, Shenyan Ma

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to explore the effects of edible fungal polysaccharides on the proliferation and metabolism of Lactobacillus rhamnosus GG (LGG ).[Method ] Flarnmulina velutipes polysaccharides (FVP ),Tremella fueiformls polysaccharides (TFP),Auricularia auricula polysaccharides (AAP ),lentinan (LNT ),Hericium erinaceus polysaccharides (HEP ),and Poria cocos polysaccharides (PCP ) were selected as carbon sources for the culture of LGG.The viable bacterial count and the concentration of short-chain fatty acids (SCFAs ) were determined.Two polysaccharides were selected from the six polysaccharides and added in different mass ratios to the LGG-fermented milk,and then the pH and textural properties of the …

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Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula, Mengying Zhou, Liujie Zheng, Ruiqing Yao, Hongyu Wu, Gangliang Hu, Zhengquan Huo, Yinhua Ni May 2025

Nutritional Components And Immunoregulatory Mechanisms Of Lactoferrin Formula, Mengying Zhou, Liujie Zheng, Ruiqing Yao, Hongyu Wu, Gangliang Hu, Zhengquan Huo, Yinhua Ni

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the immunoregulatory mechanisms of lactoferrin formula and its four nutritional components (α-lactalbumin,N-acetylneuraminic acid,yeast β-glucan,and lactoferrin ) in RAW 264.7 macrophages.[Method ] M1 and M 2 macrophage polarization were induced by lipopolysaccharides (LPS) and interleukin (IL)-4,respectively.Quantitative real-time PCR,flow cytometry,and Western blot were employed to analyze macrophage polarization and related signaling pathways.[Result ] Lactoferrin formula enhanced the phagocytic activity of macrophages and dose-dependently inhibited M 1 polarization while promoting M 2 polarization within the range of 10~50 µg/mL.A combination of four nutritional components exhibited similar effects,with greater effects than the three-component combination.Additionally,the lactoferrin formula upregulated …

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Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley, Ran Qiu, Junhui Zhong, Xiaomin Chang, Lin Xiao, Yanlong Bai, Tengfei Yu May 2025

Isolation And In Vitro Inhibition Of Mycotoxin-Metabolizing Strains From Domestic Barley, Ran Qiu, Junhui Zhong, Xiaomin Chang, Lin Xiao, Yanlong Bai, Tengfei Yu

Journal of Food Science and Biotechnology

[Objective ] This study aims to assess the effects of Fusarium on the malting process and malt quality of barley,screen out the microorganism with inhibitory activity against Fusarium,and explore the potential of this microorganism for degrading deoxynivalenol (DON ).[Method ] The mycotoxin-producing strain was isolated from domestic Dan ’er barley contaminated with pathogenic fungi,and the interaction between the pathogen and the host was studied.The germination rate,water sensitivity,and DON mass fraction were compared between the barley infected for 5 d and standard barley.The DON mass fraction,activities of protease,β-glucanase,and amylase,and physicochemical indicators (free amino acids,soluble nitrogen,and β-glucan mass concentration ) of malt …

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The Effect Of Epigallocatechin Gallate (Egcg) And Alpha L-Mangostin On Tp53, Bax, And Vim Gene Expression And Apoptosis In Ewing Sarcoma Cells, Victoria Valdez, Terry Jo Shackleford May 2025

The Effect Of Epigallocatechin Gallate (Egcg) And Alpha L-Mangostin On Tp53, Bax, And Vim Gene Expression And Apoptosis In Ewing Sarcoma Cells, Victoria Valdez, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcoma (ES) is a malignant pediatric bone tumor driven by chromosomal translocations and fusion oncogenes with limited targeted treatment options. This study investigated the chemopreventive potential of two natural compounds, Epigallocatechin Gallate (EGCG) found in green tea, and Alpha L-Mangostin from mangosteen, on apoptosis and gene expression in ES cells. We hypothesized that EGCG and Alpha L-Mangostin treatment would induce apoptosis and downregulate cancer-promoting genes. To test this, we performed tissue culture, IC50 assays, caspase-based apoptosis detection, RNA purification, cDNA synthesis, and qRT-PCR on ES cell lines treated with a high and low concentration of the two compounds. Our …

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Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function, Lu Jin, Jiansheng Jin, Yongqiang Gu, Lulu Zhou, Feifei Han May 2025

Research Progress In Effects Of Bile Acids And Their Receptors On Intestinal Function, Lu Jin, Jiansheng Jin, Yongqiang Gu, Lulu Zhou, Feifei Han

Journal of Food Science and Biotechnology

Bile acids are the main end products of cholesterol metabolism in the body,playing an important regulatory role in the digestion and metabolism of nutrients,especially lipids.The intestine is the main organ for digestion and absorption,as well as an important site for bile acids to achieve liver-intestinal circulation.Its function is closely related to the interaction between bile acids as signaling molecules and their receptors.Focusing on the metabolism and regulatory mechanism of bile acids,the author reviews the roles and underlying mechanisms of bile acids and their receptors in regulating intestinal barriers,immune function,and gut microbiota.The aim is to provide reference for related research on …

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In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight, Dingkun Zhang, Lei Wang, Ziling Yi, Beibei Wei, Deming Rao, Zhiyong Guo, Sheng Chen, Jing Wu May 2025

In Vitro Probiotic Effects Of Dextran With A Small Molecular Weight, Dingkun Zhang, Lei Wang, Ziling Yi, Beibei Wei, Deming Rao, Zhiyong Guo, Sheng Chen, Jing Wu

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to explore the effects of dextran with a small molecular weight (SDex ) on the viability of intestinal microorganisms.[Method ] The probiotic effects of laboratory-designed SDex containing only α-1,6 glycosidic bonds was evaluated by in vitro fermentation with nine typical intestinal strains,including strains from the genera Bifidobacterium and Lactobacillus,as well as species such as Escherichia coli and Enterococcus faecalis.The microbial proliferation and organic acid production during fermentation were compared among SDex,commercial dextran 20 (Dex 20),and isomalto-oligosaccharides (IMOs ).[Result ] SDex and IMOs significantly promoted the proliferation of probiotics such as Bifidobacterium and …

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Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens, Miao Zou, Jingyi Zeng, Dandan Zeng, Yuhan Fu, Xu Zhang, Bo Zhao, Tianlu Mo May 2025

Application Of Crispr/Cas System-Based Biosensors In Detection Of Foodborne Pathogens, Miao Zou, Jingyi Zeng, Dandan Zeng, Yuhan Fu, Xu Zhang, Bo Zhao, Tianlu Mo

Journal of Food Science and Biotechnology

The CRISPR/Cas system can recognize foreign DNA,cut the target,and block or silence the expression of the foreign gene.Due to its precise targeting function,the CRISPR/Cas system has been developed as a highly efficient gene editing tool.With high specificity and operability,the CRISPR/Cas system has been combined with biosensors to improve the detection specificity and efficiency of the sensors.This review introduces the characteristics and applications of CRISPR/Cas system,analyzes the latest progress in the application of CRISPR/Cas system-based biosensors in the detection of foodborne pathogens,and summarizes and prospects the application of such biosensors.

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Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou, Min Qian, Wei Zhou, Zhijin Zhou, Enbao Zhang, Jianzhong Han, Daofeng Qu May 2025

Antibiotic Resistance And Transmission Characteristics Of Enterococcus Faecalis From The Pork Production Chain In Hangzhou, Min Qian, Wei Zhou, Zhijin Zhou, Enbao Zhang, Jianzhong Han, Daofeng Qu

Journal of Food Science and Biotechnology

[Objective ] This study aims to evaluate the antibiotic resistance and transmission risk of Enterococcus faecalis (E.faecalis ) in the pork production chain.[Method ] A total of 300 samples were collected from the surface of live pigs,carcasses,and the environment in 2 pig farms,1 slaughterhouse,and 2 markets in Hangzhou,Zhejiang.The strain identification,antibiotic resistance phenotypes,resistance genotypes,and virulence genotypes were performed by 16S rDNA sequences,broth microdilution method (MIC ),and PCR.In addition,multilocus sequencing typing (MLST ) and conjugational transfer tests were conducted.[Result ] The 125 isolates of E.faecalis showed high resistance to antibiotics such as tiamulin,tetracycline,and doxycycline,and the isolates from pig farms showed higher antibiotic …

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Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation, Huahao Cai, Linlin Wang, Qingwei Yao, Xudan Shen, Xingxing Chen, Shumao Cui May 2025

Evaluation Of The Clinical Alleviation Effect Of Bacillus Coagulans Ccfm 1041 On Functional Constipation, Huahao Cai, Linlin Wang, Qingwei Yao, Xudan Shen, Xingxing Chen, Shumao Cui

Journal of Food Science and Biotechnology

[Objective ] Functional constipation is a chronic gastrointestinal disorder caused by multiple factors,with a relatively high and increasing prevalence in China.Previous studies have found that Bacillus coagulans (B.coagulans )CCFM 1041 can alleviate symptoms of functional constipation in mice.[Method ] A double-blind,randomized controlled clinical trial was conducted to evaluate the clinical efficacy of CCFM 1041 in relieving functional constipation.A total of 73 participants were observed for changes in constipation-related indicators,serum levels of gastrointestinal regulatory neurotransmitters,and gut microbiota before and after the intervention.[Result ] After taking CCFM 1041,84.2% of participants experienced increased bowel movement frequency,and 94.7% of participants showed improved stool consistency.Compared …

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