Life Sciences Commons^™

Informatics Hygiene To Support Reuse Of Routinely Collected Health Care Data For Evidence-Based Practice, Elmer V Bernstam, Alejandro Araya, Matthew Decaro, Todd R Johnson

Faculty, Staff and Student Publications

No abstract provided.

Participatory Science Newsletter 2025_05_20, Mairi Poisson, Malin Clyde, Alyson L. Eberhardt

Participatory Science News

In this edition of the UNH Participatory Sciences Newsletter, we offer news and resources on The Power of Participatory Sciences. These include a story about UNH graduate student Lauren White's work using participatory science data about horseshoe crab spawning in the Great Bay. Another story highlights UNH's Coastal Research Volunteers Beach Profiling Project and another outlines how many UNH researchers at UNH are working with local communities to find solutions to environmental problems. A final article shares information about the 2025 conference for the Association for Advancing Participatory Sciences and the poster presented by scholars and students from UNH.

Distribution Pattern And Abundance Dynamics Of Asian Openbill (Anastomus Oscitans) In Rice Field Throughout Peninsular Malaysia, Badrulhadza Amzah, Rosli Ramli, Noorhidayah Mamat

Turkish Journal of Zoology

The Asian Openbill (Anastomus oscitans) plays a crucial role in rice field ecosystems by preying on invasive apple snails (Pomacea spp.), potentially reducing the reliance on chemical molluscicides. This study investigated the distribution and abundance dynamics of the stork across major rice granaries in Peninsular Malaysia. Surveys were conducted at 111 sampling points within 30 localities across 11 states during five consecutive rice seasons (2022–2024), with bird counts obtained through binoculars and digital photography. Results indicate that storks are highly concentrated on the northern and central west coasts, where rice fields provide abundant foraging opportunities. In the northern regions (Perlis, …

Multimodal Spatial Proteomic Profiling In Acute Myeloid Leukemia, Christopher P Ly, Ivo Veletic, Christopher D Pacheco, Enes Dasdemir, Fatima Z Jelloul, Sammy Ferri-Borgogno, Akshay V Basi, Javier A Gomez, Jessica L Root, Patrick K Reville, Sonali Jindal, Sreyashi Basu, Padmanee Sharma, Andres E Quesada, Carlos Bueso-Ramos, Taghi Manshouri, Branko Cuglievan, Miriam Garcia, Jared K Burks, Hussein A Abbas

Faculty, Staff and Student Publications

Acute myeloid leukemia (AML) resides in an immune-rich microenvironment, yet, immune-based therapies have faltered in eliciting durable responses. Bridging this paradox requires a comprehensive understanding of leukemic interactions within the bone marrow microenvironment. We optimized a high-throughput tissue-microarray-based pipeline for high-plex spatial immunofluorescence and mass cytometry imaging on a single slide, capturing immune, tumor, and structural components. Using unbiased clustering on the spatial K function, we unveiled the presence of tertiary lymphoid-like aggregates in bone marrow, which we validated using spatial transcriptomics and an independent proteomics approach. We then found validated TLS signatures predictive of outcomes in AML using an …

Histone Methyltransferase Ash1l Primes Metastases And Metabolic Reprogramming Of Macrophages In The Bone Niche, Chenling Meng, Kevin Lin, Wei Shi, Hongqi Teng, Xinhai Wan, Anna Debruine, Yin Wang, Xin Liang, Javier Leo, Feiyu Chen, Qianlin Gu, Jie Zhang, Vivien Van, Kiersten L Maldonado, Boyi Gan, Li Ma, Yue Lu, Di Zhao

Faculty, Staff and Student Publications

Bone metastasis is a major cause of cancer death; however, the epigenetic determinants driving this process remain elusive. Here, we report that histone methyltransferase ASH1L is genetically amplified and is required for bone metastasis in men with prostate cancer. ASH1L rewires histone methylations and cooperates with HIF-1α to induce pro-metastatic transcriptome in invading cancer cells, resulting in monocyte differentiation into lipid-associated macrophage (LA-TAM) and enhancing their pro-tumoral phenotype in the metastatic bone niche. We identified IGF-2 as a direct target of ASH1L/HIF-1α and mediates LA-TAMs' differentiation and phenotypic changes by reprogramming oxidative phosphorylation. Pharmacologic inhibition of the ASH1L-HIF-1α-macrophages axis elicits …

Sustainability In The Cruising Industry: Innovations In Air Quality, Energy Efficiency, And Waste Management, Fikret Durmus, Mi Ran Kim

ICHRIE Research Reports

The cruise industry, a cornerstone of the global hospitality and tourism sector, faces increasing scrutiny over its environmental impact. As passenger numbers grow, so does the industry's responsibility to adopt sustainable practices. This report examines key innovations in air quality management, energy efficiency, and waste management, highlighting the industry's transition from historically high emissions and waste production to advanced sustainability initiatives. Key focus areas include evolving maritime regulations, adopting cleaner propulsion technologies, integrating energy-efficient solutions, and improving waste treatment practices. Findings indicate that industry leaders are investing in liquefied natural gas engines, exhaust gas cleaning systems, and onshore power supply …

Engineering Hiv-1 Env Protein To Elucidate In Situ Structural Dynamics Of Env, Narendra Kumar Gonepudi

Biotechnology Theses

The HIV-1 Envelope (Env) glycoprotein trimers undergo structural changes upon interacting with host receptor CD4 and coreceptors (CCR5/CXCR4) for virus entry. As the only surface-exposed viral protein, Env is a key antibody target but evades recognition through structural changes. Studying Env dynamics with respect to the coreceptor binding region (V3 loop) provides insights into virus entry and immune evasion. Fluorescence labeling via genetic code expansion (amber–TAG suppression) offers a minimally invasive approach for fluorescence microscope-based Env dynamics studies. Here, two V3-related dual-amber Env constructs (A135_TAG-P308_TAG, P308_TAG-E395_TAG) were generated by incorporating two non-canonical …

Dynamical Evolution Of Kinematic Properties: The Steady Forcing Case, A.D. Kirwan, Jr, Helga Huntley, James Turbett

College of Science & Mathematics Departmental Research

Observations from recent large submesoscale drifter deployments in the ocean exhibit complicated time series of the kinematic properties (KP) — divergence, vorticity, shear and normal strain rates. These are challenging to interpret in the complex ocean-atmosphere system. The evolution equations for the four KP are derived from the two-dimensional momentum conservation equations on the f -plane. The resulting equations capture the submesoscale motion of drifters along fixed ocean surfaces, with time and space scales on the order of days and kilometers. They are linearly coupled through the Coriolis term and nonlinearly coupled through the horizontal divergence. The solution space, even …

Lethal And Sublethal Effects Of Cotton Expressing Single And Pyramided Proteins Of Bacillus Thuringiensis (Bt) On Helicoverpa Armigera (Lepidoptera: Noctuidae), Spodoptera Litura (Lepidoptera: Noctuidae), And Spodoptera Frugiperda (Lepidoptera: Noctuidae), Sharna Holman, Paul Grundy, Helen Spafford, Michael Furlong

Grain and Other Field Crops Research Articles

The susceptibility to proteins from Bacillus thuringiensis (Bt) can vary among lepidopteran pest species. While Bollgard 3 cotton (BG3) effectively controls the primary pest Helicoverpa armigera (Hübner) in Australia, its effectiveness against other pests, such as Spodoptera litura (Fabricius) and Spodoptera frugiperda (J. E. Smith), is unknown. This laboratory study assessed the survival and development of H. armigera, S. litura, and S. frugiperda larvae when fed foliage from a non-transgenic cotton variety (CC) and 3 transgenic cotton varieties: Bollgard (BG1) expressing Cry1Ac, Bollgard II (BG2) expressing Cry1Ac and Cry2Ab, and Bollgard 3 (BG3) expressing Cry1Ac, Cry2Ab, and Vip3A. Pyramided …

Thousand Cankers Disease Of Walnut (Geosmithia Morbida), Claudia Nischwitz, Marion Murray

All Current Publications

No abstract provided.

Mate Choice And Movement Ecology Of Plegadis Ibis In Louisiana, Ryan T. Klutts

LSU Master's Theses

In 1817, George Ord documented the first North American records of Glossy Ibis (Plegadis falcinellus), a species native to the Old World. Over the next 200 years, Glossy Ibis expanded its breeding range to include the Atlantic coast to southernmost Florida and thence up the Gulf coast to eastern Texas. Forty-five to 75 years ago, its range expansion brought it into secondary contact with White-faced Ibis (P. chihi) in Louisiana, a native species whose breeding range includes freshwater wetlands in Louisiana and eastern Texas. Hybridization between the two species resulted in a large hybrid zone in southern Louisiana, providing an …

Sequence Conservation Of Suppressor Of Ikk Epsilon Phosphorylation Sites, Kaydence M. Isaacs

Undergraduate Honors Theses

Suppressor of IKK epsilon (SIKE) is a protein that is implicated in immune response signaling pathways. SIKE interaction networks suggest roles in cell motility and macromolecular complexes mediating kinase/phosphatase activity. In this project, we aim to better understand the function of SIKE by analyzing amino acid sequences across different species. In human SIKE, viral infection leads to phosphorylation of SIKE at defined serine residues. Comparing SIKE sequences, and specifically these phosphorylation sites, allows us to discover its ancestral connections to innate immune function. This has particular applications in bridging the evolutionary gap between fungi and metazoa SIKE sequences. Multiple sequence …

Myocardin Effector Proteins Affect Tgf-Beta-Induced Myofibroblast Differentiation In Ipf Cells, Allison Gonzalez

Biotechnology Theses

Idiopathic pulmonary fibrosis (IPF) is an incurable interstitial lung disease defined by the progressive, irreversible scarring of the tissues between the lung’s alveoli. To date, only two drugs have received approval for treatment. However, these medications are not considered cures since they do not stop the progression of the disease. A deeper understanding of the mechanisms underlying IPF could aid in discovering a cure. We recently found that myocardin (MyoCD) regulates SMAD2/3 transcription factors in the TGF-β signaling pathway and governs the genes involved in pleural fibrosis. However, it is unclear whether MyoCD plays a critical role in IPF by …

A Fundamentally New Direction In Embolization Using Reactive Chemistry In A Swine Model, Erik Cressman, Danielle Stolley, Shubhneet Warar, Natalie W Fowlkes, David Fuentes

Faculty, Staff and Student Publications

Liver cancer carries a poor prognosis and incidence continues to increase. The main therapy for unresectable disease > 3 cm in diameter is Transarterial Chemoembolization. Unfortunately, overall survival for these patients has improved little in the past two decades. To address this, we propose a new approach using a chemical reaction in situ. We report here our results in a pilot study using a swine model. Domestic swine (n = 3) were treated in the liver with dichloroacetic anhydride in ethiodized oil. CT imaging was followed 24 h after the procedure by necropsy, histopathology, and mass spectrometry imaging. Animals tolerated the …

Application Of Bioinformatic Tools To Tuberculosis Surveillance At The Arkansas Department Of Health, Brian Scott Delavan

Theses and Dissertations

Tuberculosis (TB) remains a public health threat in Arkansas. Great strides have been made over the past fifty years to reduce the number of TB cases in Arkansas. However, the rate of TB cases has remained steady since 2010. According to the Arkansas Department of Health, from 2012 through 2023 the TB Case rate per 100,00 population was between 2.0 and 3.1. To continue Arkansas’ drive towards elimination of TB in Arkansas, defined as one TB case per million residents, new data analysis tools are needed. This project employs multiple bioinformatic tools to analyze TB cases and latent tuberculosis infections …

Transcriptome Analysis Reveals Nutrient Deprivation Reduces Nitrate Content In Lettuce (Lactuca Sativa Var. Ramosa Hort.) And Enhances Nitrogen Metabolism, Yingying Zhang, Xuena Liu, Shikai La, Mingjiao Wang, Xu Hu, Ainoug Shi, Jinghuai Guo, Lingdi Dong

Horticulture Faculty Publications and Presentations

Introduction Excessive nitrate accumulation in leafy vegetables poses health risks and indicates inefficient nitrogen use in conventional agriculture. While nitrogen metabolism has been extensively studied, the molecular mechanisms linking nutrient deprivation to nitrate reduction in vegetables remain unclear. Methods In this study, we investigated these mechanisms in lettuce (Lactuca sativa var. ramosa Hort.) by analyzing nitrate content and gene expression in leaves and roots under nutrient deprivation. Results After five days of treatment, nitrate content decreased by 57.49% in leaves and 50.77% in roots. Transcriptome sequencing identified 323 and 3,494 differentially expressed genes (DEGs) in leaves and roots, respectively, with …

Development And Evaluation Of A Simple Human Body Link Model Considering The Degrees-Of-Freedom Of Shoulders, Mizuki Takeda, Yuki Saito, Kaiji Sato

Progress in Scale Modeling, an International Journal

Modeling of the human body is widely utilized in the field of human–robot interaction, warranting the development of a simple model to measure and recognize the human body movements. To this end, a two-dimensional (2D) human body link model in the sagittal plane has been used to represent the human body with rotating joints and links connecting these joints. The joint positions can be determined by using the coordinates of a limited number of points on the links and estimated using a few sensor outputs when the links are of constant length. However, models with constant link lengths may result …

Observing The Prevalence Of Wolbachia Found In Tick Species From The Central Virginia Region, Madison Hoover

Undergraduate Theses and Capstone Projects

Research has found that ticks are considered the second highest global vector of disease. Ticks can serve as a vector for pathogens such as Rickettsia rickettsii (Rocky Mountain Spotted Fever), Borrelia burgdorferi (Lyme Disease), Borrelia miyamotoi (B. miyamotoi disease) and Borrelia hermsii (tick-borne relapsing fever). Tick species such as Dermacentor variabilis (American Dog ticks), Amblyomma maculatum (Gulf Coast ticks), Amblyomma americanum (Lone Star ticks), Ixodes scapulari (Black legged ticks), and Ixodes affinis can be found within the southeastern Virginia region. Arthropod hosts that possess Wolbachia can have their reproduction negatively impacted through methods such as Parthenogenesis, Male Killing, Feminization, and …

An Examination Of The Effect Of Cloth Material On The Efficiency And Success Of Tick Dragging Protocol In Lynchburg Va., Hyden Draughon

Undergraduate Theses and Capstone Projects

Ticks serve as a major vector of arthropod-borne pathogens that could affect human populations. Ixodes scapularis, Amblyomma americanum, Dermacentor variabilis, and Haemaphysalis longicornis populations have been commonly reported in the state of Virginia, and can carry pathogens potentially fatal to humans. I. scapularis serves as a vector of Borrelia burgdorferi (the bacterium that causes Lyme disease), A. americanum has been found to carry Ehrlichia chaffeensis (the bacterium that causes Human Monocytic Ehrlichiosis), D. variabilis can carry Rickettsia rickettsii (the bacterium that causes Rocky Mountain Spotted Fever), and H. longicornis has been found to transmit protozoan Theileria orientalis to livestock. Over …

A Phylogenomic Investigation Of The Historical Biogeography Of Livebearers (Cyprinodontiformes: Poeciliidae), Sheila Rodríguez Machado

LSU Doctoral Dissertations

The Neotropics contain several biodiversity hotspots and harbor the highest diversity of freshwater fishes globally. Yet, recognizing the patterns and processes driving this richness remains a challenge. Similarly, all groups and subregions have not received the same attention, especially in the genomics era. Addressing these knowledge gaps will help us to better understand the region’s evolutionary and biogeographic complexity. The goal of this dissertation is to examine phylogenetic and biogeographic patterns of Neotropical freshwater fishes, with emphasis on the Greater Antilles, across multiple taxonomic and geographic scales. To achieve this goal, I generated a genomic dataset of hundreds of ultraconserved …

Pollinator Plant Communities Within Restored Montane Longleaf Pine Ecosystems, Hanen Van Rossem

Master's Theses

Historically the fire-dependent and biodiverse longleaf pine (Pinus palustris) ecosystem was the dominant ecosystem of the Southeastern US. This ecosystem’s range was massively reduced due to land conversion and fire exclusion, causing the decline of many native species. Efforts to restore and protect longleaf pine stands are now ongoing with the goal of expanding habitat and refuge for threatened species. Differences in restoration practices and prior land use may influence the extent to which restored habitat patches can support pollinators and their plants. This study compared pollinator plant biodiversity between restored neighboring montane longleaf pine stands that differed in …

Oviposition And Immature Development Of Blowflies In Forensic Investigations, Amalia Herrera-Benitez

Themis: Research Journal of Justice Studies and Forensic Science

Forensic entomology is a subspecialty within the field of forensic science where insect evidence is analyzed to solve criminal cases. Insects begin to colonize the body, with blowflies being the first insects to arrive. Within minutes, blowflies can lay eggs on a decomposing body, and the larvae will feed once they hatch. The life cycles of blowflies are analyzed to help determine an estimated post-mortem interval (PMI), which is crucial in investigations, as it can help establish a timeline. Various factors, such as temperature and access to the body, can cause delays in the oviposition of blowflies. A delay in …

Causes Of Heterozygosity Excess: The Case Of Mexican Populations Of Populus Tremuloides, Javier Hernández-Velasco, José Ciro Hernández-Díaz, Sergio Leonel Simental-Rodríguez, Juan P. Jaramillo-Correa, David S. Gernandt, José Jesús Vargas-Hernández, Ilga Porth, Roos Goessen, M. Socorro Gonzáles-Elizondo, Matthias Fladung, Cuauhtémoc Sáenz-Romero, José Guadalupe Martínez-Ávalos, Artemio Carrillo-Parra, Eduardo Mendoza-Maya, Arnulfo Blanco-García, Christian Wehenkel

Aspen Bibliography

The presence of heterozygous individuals in a population is crucial for maintaining genetic diversity, which can positively affect fitness and adaptability to environmental changes. While inbreeding generally reduces the proportion of heterozygous individuals in a population, polyploidy tends to increase the proportion. North American Populus tremuloides is one of the most widely distributed and ecologically important tree species in the Northern Hemisphere. However, genetic variation in Mexican populations of P. tremuloides, including the genetic signatures of their adaptation to a variety of environments, remains largely uncharacterized. The aim of this study was to analyze how inbreeding coefficient (F …

The State Of Municipal Scale Highest Use Urban Wood Reclamation Projects, Bradley C. Almond

Graduate Liberal Studies Theses and Dissertations

Millions of urban trees are downed annually for various reasons, such as storms, disease, or development. We are all witnesses to the loss of trees as we see the piles of woody waste assembled on the curbside, ready for public works pickup. Today, most urban wood waste ends up in landfills, mulching/chipping facilities, or firewood production. Each of these alternatives results in the release of sequestered carbon back into the atmosphere.

The initial research led to successful diversion from landfills to mulch and firewood operations. Then, the research shifted to converting urban wood waste for its best use, which outlined …

Computational Identification Of Infiltrating T Lymphocytes Into Solid Tumor, Lin Zhong

Statistical Science Theses and Dissertations

T cells play a central role in cancer immunity. Successful anti-tumor immunotherapy relies on the effective infiltration of cytotoxic T cells. However, the tumor microenvironment presents physical and immunosuppressive barriers to prevent T cell entry. Here, we developed two novel computational tools, ReMITT and SIBERT, to search for migrating T cells and T cell entry regions (TERs) into the tumor core. SIBERT combines a novel change point detection method and a spatial imputation model to detect regions with signatures of recent T cell entry using the spatial transcriptomics data. Applying SIBERT to multiple human cancer samples revealed several TERs for …

Assessing The Efficiency Of Methotrexate (Mtx) As An Antiviral Drug Against Gammaherpes Virus Replication, Yennifer A. Gaspar Garcia

Honors Projects

It is estimated that ~15% of all cancers are caused by oncogenic virus infections. Two of the top seven cancer-causing human viruses are members of the gammaherpesvirus family: Epstein Barr Virus (EBV) and Kaposi’s Sarcoma Herpesvirus (KSHV). Our lab uses Murine Herpesvirus 68 (MHV-68), a mouse gammaherpesvirus with shares significant genetic homology to KSHV and EBV, as a model system to understand how gammaherpesviruses alter the metabolism of their host during lytic infection to promote their replication. We recently metabolically profiled MHV-68 infected host cells at various time points during the lytic infectious cycle. Our data showed nucleotide metabolism is …

Sex, Skulls, And Selection: An Exploration Of Blakely Island's Deer Population And Their Ecology Through Genetic Sex Differentiation Of Juvenile Crania, Blair P. Graves

Honors Projects

As an isolated landmass with no natural predators, Blakely Island serves as an effective study site for the study of evolutionary factors relating to insular populations of large mammals. A disparity in the adult sex ratio of local deer has been observed, but the demographics of juveniles must still be ascertained through genetic methods. This study aims to further research concerning the development of a replicable and non-toxic procedure through which undergraduate students can identify the sex of numerous juvenile deer crania. Specifically, differing decalcification lengths, DNA extraction kit methods, and amelogenin primer sets were evaluated for relative success in …

Endophenotype-Informed Association Analyses For Liver Fat Accumulation And Metabolic Dysfunction In The Fels Longitudinal Study, Ariana L. Garza, John Blangero, Miryoung Lee, Cici X. Bauer, Stefan A. Czerwinski, Audrey Choh

School of Medicine Publications

The identification of causal genomic regions for liver fat accumulation in the context of metabolic dysfunction remains a challenging goal. This study aimed to identify potential endophenotypes for liver fat content and employ them in bivariate linkage searches for pleiotropic genetic regions where targeted association analysis is more likely to reveal significant variants. Multiple metabolic risk and adiposity distribution traits were assessed using the endophenotype ranking value. The top-ranked endophenotypes were then used in a bivariate linkage analysis, paired with liver fat content. Quantitative trait loci (QTLs) identified as significant or suggestive were targeted for measured genotype association analyses. The …

A Floristic Study Of The Vascular Flora Of The Brokeoff Mountains, Southcentral New Mexico, Bryana L. Olmeda, Harpo Faust, Hannah E. Marx

Biology ETDs

The vascular flora detailed here describes 438 km² (169 mi²) of the Brokeoff Mountains of southeastern New Mexico. The Brokeoffs are part of the northern extent of the Chihuahuan Desert, the largest desert in North America and one of the ecoregions least represented in botanical collections. Prior to 2023, only 254 plant collections were known from the study site, representing roughly 1.5 collections per 2.59 km² (1 mi²), despite strong research interest in its neighboring Guadalupe Mountains. Over two field seasons, 1920 plant collections were made, over 81 days of field work conducted, in …

Neoadjuvant With Low-Dose Radiotherapy, Tislelizumab, Albumin-Bound Paclitaxel, And Cisplatin For Resectable Locally Advanced Head And Neck Squamous Cell Carcinoma: Phase Ii Single-Arm Trial, Zhigang Liu, Dong Wang, Guanjun Li, Muhua Yi, Zhaoyuan Zhang, Guihua Zhong, Liangfu Xu, Rong Jiang, Yannan Zheng, Linxuan Huang, Yingpeng Peng, Lizhong Liang, Jianpeng Li, Ye Liu, Jun Lai, Xianjuan Lv, Yongqiang Xu, Qiaodan Liu, Zhiqiang Wang, Zhutian Liu, Qinan Yang, Li Nie, Jiao Lei, Xiaotao Huang, Zhijie Liu, Wen Jiang

Faculty, Staff and Student Publications

Although pathological complete response (pCR) and major pathological response (MPR) rates of neoadjuvant immunotherapy combined with chemotherapy in head and neck squamous cell carcinoma (HNSCC) trials remain suboptimal, emerging evidence highlights the synergistic potential of combining low-dose radiotherapy with immunotherapy to promote the efficacy of immunotherapy. This phase II, open-label, single-arm, multicenter trial (NCT05343325) enrolled 28 patients with untreated stage III-IVB HNSCC (NeoRTPC02). Patients received neoadjuvant low-dose radiotherapy, the programmed death-1 (PD-1) inhibitor tislelizumab, albumin-bound paclitaxel, and cisplatin for two cycles, followed by radical resection ~4 weeks after treatment completion. The primary endpoint, pCR rate, was achieved in 14 of …