Life Sciences Commons^™

Src/Fn1 Pathway Activation Drives Tumor Cell Cluster Formation And Metastasis In Lung Cancer: A Promising Therapeutic Target, Zujun Que, Zhichao Xi, Dan Qi, Rongchen Dai, Yang Li, Mengfan Liu, Bin Luo, Jiajun Liu, Pan Yu, Yun Yang, Erxi Wu, Hongxi Xu, Jianhui Tian

Children’s Nutrition Research Center Staff Publications

Lung cancer remains the leading cause of cancer-related death globally, with metastasis driven by circulating tumor cells (CTCs)-particularly clusters-being a major treatment challenge. Despite their critical role, the biological differences between single CTCs and CTC clusters remain unclear. Here, we comprehensively compared their behavioral, transcriptomic, and proteomic profiles in lung cancer models. Compared with single cells, CTC clusters present enhanced metastatic potential, greater survival in the bloodstream and increased resistance to microenvironment. Mechanistically, the Src/FN1 pathway is centrally activated in clusters, promoting intercellular cohesion and protecting against immune clearance and stress in circulation. Pharmacological inhibition of Src with the clinical …

Bridging Prairie Restoration And Regenerative Agriculture With Compost Tea, April Hersey

2025 Symposium

Inland Pacific Northwest prairie is an endangered ecosystem that has been reduced by conversion to agriculture. There is growing interest in the role of soil microorganisms in prairie ecosystems, which may be crucial for restoration of the plant community through interspecies interactions within the soil near plant roots. One method for improving soil microorganism community composition is compost tea: enriched liquid compost extract. Compost tea is used in regenerative agriculture to improve crop yields and protect against pathogens, ostensibly through the activity of the added compost microbes. While research has supported compost tea application in regenerative agriculture, it has not …

To Play Or Not To Play: Investigating The Effects Of Auditory Stimuli During Running, Anna L. Tamblyn, Jodi Brandt, Chloe Schmidt, Grace Barnett, Danielle Kern

2025 Symposium

Research has shown that listening to music during physical activity can have a positive effect on emotional state, ratings of perceived exertion, and overall performance. However, limited research has investigated the differences between tempo-matched music and metronome pacing on running performance. Many runners utilize metronome pacing to pace a run, improve efficiency, and enhance form. To date, research on the efficacy of metronome pacing compared to music on one mile run time to completion is limited. PURPOSE: To determine the effects of different auditory stimuli (e.g., music, metronome, and silence) on one mile run time in young adults. METHODS: Young …

Niche Conservatism And Sympatric Parallel Evolution May Help To Maintain Eight Nascent Tree Taxa Along A Sharp Elevation Gradient, Elizabeth A. Stacy, Rebecca Ostertag

Life Sciences Faculty Research

1. Patterns of niche partitioning among closely related taxa, such as those in incipient adaptive radiations, can provide clues into how niches are created, partitioned, and integrated over ecological and evolutionary time scales. Hawaiian Metrosideros is a ~3-MYO adaptive radiation of trees that dominates the Hawaiian Islands in continuous stands within islands and allows examination of niche partitioning at the early stages of adaptive radiation.
2. We monitored growth and survivorship over 2 years of seedlings derived from open-pollinated fruits of eight Metrosideros taxa distributed along the elevation gradient of Koʻolau Volcano, Oʻahu, under four combinations of light and phosphorus. We included …

First Detection And Tracing Of Grapevine Red Blotch Virus (Grbv) In Australia Using Tiled Amplicon Sequencing, Tonny Kinene, Andrew S. Taylor, Richard Fennessy, Brenda A. Coutts, David Lovelock, Cuiping Wang, Kamalpreet Kaur, Thao Tran, Brendan Rodoni, Fiona E. Constable, Monica A. Kehoe

Viticulture Research Articles

Grapevine red blotch virus (GRBV; species Grablovirus vitis, genus Grablovirus, family Geminiviridae) poses a significant threat to viticulture worldwide, impacting grapevine health and wine quality. Here, we report the first detection and tracing of GRBV in Australia and provide a summary of a subsequent survey to determine the extent of GRBV distribution in Western Australia. Additionally, the study introduces a tiled amplicon sequencing method, which, when combined with long-read nanopore sequencing, enables rapid GRBV genome sequencing. Our analysis suggests a single introduction of GRBV from North America to Australia through the state of Victoria and subsequently to …

Differences In Children’S Food And Beverage Consumption Between School And Summer: Three-Year Findings From The What’S Up (Undermining Prevention) With Summer Observational Cohort Study, Michael Beets Med, Mph, Phd, Sarah Burkart, Christopher D. Pfledderer, Elizabeth L. Adams Phd, Robert Weaver Med, Phd, Bridget Armstrong, Keith Brazendale, Xuanxuan Zhu, Alexander Mclain, Gabrielle Turner-Mcgrievy Ph.D., Rd, Russell R. Pate, Andrew T. Kaczynski, Amanda Fairchild, Brian Saelens, Hannah Parker, Amy L. Yaroch, Emily Eglitis, Anthony J. Holmes

Faculty Publications

Background

Summer vacation is a time when youth gain excessive weight. A key driver of unhealthy weight gain is poor dietary quality. The absence of consistent structure (e.g., school), is hypothesized to be one of the reasons for lower diet quality during summer. This study examined differences in school and summer dietary quality among a diverse cohort of children across three years. We also examined the impact of attending structured programs on children’s diets.

Methods

Parents of 1,032 children (age 5–14 years, 48% girls) completed a time use diary each day for 14-days during school (April/May) and again in summer …

Identification Of Serum Exosome Proteins In Systemic Sclerosis With Interstitial Lung Disease By Aptamer Proteomics, Sonsoles Piera-Velazquez, Simon T. Dillon, Xuesong Gu, Towia A. Libermann, Sergio A. Jimenez

Jefferson Institute of Molecular Medicine Papers and Presentations

OBJECTIVE: A major unmet need for Systemic Sclerosis (SSc) clinical management is the absence of well validated biomarkers for early diagnosis of SSc-associated interstitial lung disease (SSc-ILD). The objective of this study was to identify proteins contained within serum exosomes that may serve as potential biomarkers to differentiate patients with Diffuse SSc without SSc-ILD from patients with Diffuse SSc with SSc-ILD employing aptamer-based proteomics.

METHODS: Serum exosomes were isolated from two cohorts of patients. The first cohort included 15 patients with Diffuse SSc without SSc-ILD and 14 patients with Diffuse SSc with SSc-ILD and the second cohort included 12 patients …

Meta-Analysis Of Uveal Melanoma Genome-Wide Association Studies Identifies Novel Risk Loci And Population Effect Size Heterogeneity., Georgia Mies, Noah L. Tsao, Alexandre Houy, Sarah E. Coupland, Helen Kalirai, Asta Försti, Kari Hemminki, Hauke Thomsen, Marc-Henri Stern, Carol L. Shields, Scott M. Damrauer, Katheryn G. Ewens, Arupa Ganguly, Iain Mathieson

Wills Eye Hospital Papers

Uveal melanoma (UM) is a rare but frequently metastasizing cancer. Genome-wide association studies have identified three common genome-wide significant germline risk loci. Here, we perform a genome-wide association study on 401 new cases and conduct a meta-analysis with three independent previously published cohorts for a total sample size of 2,426 cases. We confirm the three previously identified risk loci and identify four additional genome-wide significant loci. We find that eye pigmentation-decreasing variants are systematically associated with increased UM risk and that selection for lighter pigmentation in the past 5,000 years explains about 73% of the difference in UM incidence between …

Oregon State Rank Assessment For Howell's Sandwort (Sabulina Howellii), Lindsey K. Wise, Nora Dunkirk

Institute for Natural Resources Publications

Oregon state conservation status assessment for Howell's sandwort (Sabulina howellii) using NatureServe methodology, 2025

Salinity And Moisture Influence Co2 And Ch4 Emissions From High-Latitude Coastal Soils, B. N. Barr, K. C. Kelsey, A. J. Leffler, M. Petit Bon, K. H. Beard

Wildland Resources Faculty Publications

Sea level rise and more frequent and larger storms will increase saltwater flooding in coastal terrestrial ecosystems, altering soil-atmosphere CO₂ and CH₄ exchange. Understanding these impacts is particularly relevant in high-latitude coastal soils that hold large carbon stocks but where the interaction of salinity and moisture on greenhouse gas flux remains unexplored. Here, we quantified the effects of salinity and moisture on CO₂ and CH₄ fluxes from low-Arctic coastal soils from three landscape positions (two Wetlands and Upland Tundra) distinguished by elevation, flooding frequency, soil characteristics, and vegetation. We used a full factorial laboratory incubation experiment …

The Impacts Of Ocean Warming On Phytoplankton Dynamics And Links To Fisheries Management Around St Helena Island, South Atlantic Ocean, Shelby Bargo

The Plymouth Student Scientist

In the context of a rapidly changing climate, understanding how ocean warming is reshaping the foundation of marine ecosystems has never been more important. This research investigates factors contributing to ocean warming, and where anthropogenically influenced climate change plays a critical role in the functionality of the physical marine environment, phytoplankton, and trophodynamics throughout the pelagic zone. Phytoplankton, whilst microscopic, are vital; they are regulated by physical oceanographic factors that control their access to growth-limiting factors, such as nutrients. With the formidable climate-driven physical and biological changes occurring in the marine environment, alongside the decline in fish stocks, it is …

Oregon State Rank Assessment For Limber Pine (Pinus Flexilis), Lindsey K. Wise

Institute for Natural Resources Publications

Oregon state conservation status assessment for limber pine (Pinus flexilis) using NatureServe methodology, 2025.

Blunted Cd40-Responsive Enhancer Activation In Crebbp-Mutant Lymphomas Can Be Restored By Enforced Cd4 T-Cell Engagement, Haopeng Yang, Wenchao Zhang, Vida Ravanmehr, Guiling Cui, Kevin Bowman, Ruidong Chen, Jared M Henderson, Shyanne Lockman, Estela Rojas, Ashley Wilson, Sydney Parsons, Ariel Mechaly, Leslie Regad, Ahmed Haouz, Christopher R Flowers, Sattva Neelapu, Loretta Nastoupil, R Eric Davis, Qing Deng, Fernando Rodrigues-Lima, Michael R Green

Faculty, Staff and Student Publications

The CREBBP lysine acetyltransferase (KAT) is frequently mutated in follicular lymphoma and diffuse large B-cell lymphoma and has been studied using gene knockout in murine and human cells. However, most CREBBP mutations encode amino acid substitutions within the catalytic KAT domain (CREBBP KAT-PM) that retain an inactive protein and have not been extensively characterized. Using CRISPR gene editing and extensive epigenomic characterization of lymphoma cell lines, we found that CREBBP KAT-PM lead to unloading of CREBBP from chromatin, loss of enhancer acetylation, and prevention of EP300 compensation. These enhancers were enriched for those that are dynamically loaded by CREBBP in …

Bigapp: A User-Friendly Genomic Tool Kit Identified Quantitative Trait Loci For Creeping Rootedness In Alfalfa (Medicago Sativa L.), Alexander M. Sandercock, Michael D. Peel, Cristiane H. Taniguti, Josué Chinchilla-Vargas, Shufen Chen, Manoj Sapkota, Meng Lin, Dongyan Zhao, Arlyn J. Ackerman, Bhoja R. Basnet, Craig T. Beil, Moira J. Sheehan

Plants, Soils and Climate Student Research

Alfalfa (Medicago sativa L.) is a globally vital forage crop valued for its perennial growth and multiple annual harvests. A breeding effort is underway to improve the crop for productivity and persistence against biotic and abiotic stresses using “creeping rootedness,” a trait where plants exhibit horizontal root growth, similar to rhizomes, with increased vegetative ground surface area. In this study, we genotyped a breeding population of 648 alfalfa lines segregating for creeping rootedness using the 3K DArTag marker panel to identify trait-associated genomic loci and evaluate the feasibility of genomic prediction to accelerate breeding cycles. Using genome-wide association studies …

Weaning Beef Calves, David Secrist

All Current Publications

Weaning a beef calf is likely the most stressful event in the animal’s life. Future growth and reproductive performance can be compromised by health challenges encountered through the weaning process. Consequently, proper management aimed at reducing stress and preparing the beef animal for the next stage of production is critical to reach its genetic potential. Proper management involves applying good animal husbandry techniques, using sound science in developing health and feeding protocols, and implementing low-stress animal handling practices.

The Impact Of Genetic Ancestry On Survival Outcomes In Pediatric Rhabdomyosarcoma: A Report From The Children’S Oncology Group, Ekene A Onwuka, Christina L Magyar, Bailey A Martin-Giacalone, Michael E Scheurer, Deborah A Marquez-Do, Mark Zobeck, Elizabeth G Atkinson, Erin R Rudzinski, Michael A Arnold, Donald A Barkauskas, David Hall, Javed Khan, Jack F Shern, Paul Scheet, Brian Crompton, Corinne M Linardic, Douglas S Hawkins, Rajkumar Venkatramani, Lisa Mirabello, Chad D Huff, Melissa A Richard, Philip J Lupo

Faculty, Staff and Student Publications

Emerging evidence suggests genetic ancestry may influence childhood cancer outcomes, but its impact on pediatric rhabdomyosarcoma (RMS) is unknown. We explored genetic ancestry's impact on survival among children with RMS. This multi-center observational cohort study is a secondary analysis of previously collected biobanking, genomic, and clinical data. The study included 920 individuals with newly diagnosed RMS under 40 years of age enrolled from 2005 to 2017 under the COG soft tissue sarcoma biobanking protocol D9902. The primary endpoints were (1) event-free survival (EFS), defined as the time from study enrollment to tumor recurrence/progression, secondary malignancy, or death from any cause; …

Fireworks And Wood Combustion Impacts On Air Quality, Iris Keller

DePaul Discoveries

Fire is used as a land management tool to improve ecosystem health and to reduce wildfire risk by lowering tree and litter density. However, prescribed fire as a management practice has faced difficulties with public perception, because of increased awareness of air pollution. Understanding the way that prescribed burns impact air quality and how beneficial they are for ecosystems can help the public to recognize their necessity. This study measured particulate matter (PM) emissions from prescribed burns but also considered other PM sources: fireworks and bonfires. The measurements were conducted in Minnesota, Wisconsin, and the Chicagoland area in the summer …

Factors Motivating Clothing Choice: Environmental Impact Of T-Shirts, Lauren Hunt

DePaul Discoveries

Second only to oil, the fashion industry is one of the most pollutive industries globally. Due to the rise of fast fashion, this environmental issue is continually growing. The level of production has dramatically increased while quality of apparel has decreased, causing shorter garment lifespans. This research utilizes life cycle assessment to identify the environmental impacts of various textiles used in the production of t-shirts, including cotton, polyester, viscose, and elastane. The research scope focuses from material extraction through garment disposal. The life cycle assessment research is paired with a community survey to determine consumer behavioral patterns surrounding clothing consumption …

Environmental Justice And Soil Lead Contamination: Spatial Analysis Across Seven Community Areas, Arsene Lionel Apabaga

DePaul Discoveries

Pb is a pervasive metal with toxicity linked to numerous human health issues. Urban soil is a well-documented environmental sink for Pb, persisting as a legacy pollutant decades after the phaseout of leaded gasoline. This study measured, mapped, and analyzed the spatial distribution of total soil Pb concentrations across seven Chicago Community Areas (CCAs). The objectives were to: (1) assess the relationship between mean soil Pb concentration and mean Community Environmental Justice Index (CEJI) score per CCA, (2) determine the relationship between soil Pb levels and the percentage of industrial land use, and (3) quantify and compare the percentage of …

Values And Barriers: A Multidimensional View Of College Students’ Motivations To Volunteer, Gavin Warner, Quang Anh Nguyen, Ansuk Jeong

DePaul Discoveries

University students tend to have ample access to many resources for volunteering and so are in a unique position among Americans. With all the benefits of volunteering outlined in past research, the students training to become leaders in their fields may have the most to gain from volunteerism. It is surprising, then, to note that young people are less likely to volunteer than other age groups. Why is it that college students tend to volunteer at such low rates? The current study seeks to answer this question through a mixed-methods survey. A qualitative section clarified which specific barriers students felt …

“Put Me In Coach!”: The Impact Of College Sport Team’S Mentorship In Chronically Ill And Disabled Children And Adolescents Adherence To Medical Regimen, Jameela Askira

DePaul Discoveries

The purpose of this study was to see if participation in TeamIMPACT, a program which matches ill children with college sports teams, aided chronically ill children and adolescents in adhering to their treatment regimen. This research is a part of a larger study where 25 child participants were interviewed via Zoom to answer questions regarding their time in the program, peer and family relationships, coping and resilience and other factors. The current study solely analyzed answers to questions regarding adherence to medical treatments. There were three measures: Child vs Parent Response, Treatment Regimen Components and Care Responsibility. Through a descriptive …

Using Synchrotron Wide-Angle X-Ray Scattering To Study Collagen Degradation In Second Metacarpal Bones, Zeel Sheth, Misha Romanov, Ruby Schwieger

DePaul Discoveries

The study examines female human second metacarpal (mc2) bones from early medieval cemetery at Greding, Germany, using high-energy Wide-Angle X-ray Scattering (WAXS) at beamline 1-ID of the Advanced Photon Source (APS) at Argonne National Laboratory. Rietveld refinement showed that carbonated apatite lattice parameters did not correlate with estimated age-at-death. For six of the mc2, widths of 00.2 and 00.4 diffraction peaks were substantially narrower than those of a modern (non-diagenetically altered) mc2 and of the other four ancient mc2. The peak width results are interpreted as indicating that four of the ten ancient mc2 retain substantial collagen and six of …

Modified 2-Quinolinones For Photocatalysis: The Fluorescence Quantum Yield (Φf) Of Novel 2-Quinolinone Derivatives, Reggina Moreno Trevino, Graham Griffin

DePaul Discoveries

The photophysical properties of novel 2-quinolinone derivatives were examined to evaluate their potential as photocatalysts using fluorescence and UV-Vis absorbance spectroscopy. The fluorescence quantum yields were determined via the relative comparative method, using target absorbance values at a specific wavelength to prepare solutions and comparing these measurements to the reference molecule carbostyril-124. Molar absorptivity values were also calculated to assess the light absorption properties for each compound. Among the derivatives, PAV-3 presented with the highest molar absorptivity and fluorescence quantum yield values (ε= 15297.3 M-1 cm-1, ΦF= 17.1%), while PAV-5 showed the lowest (ε= 4796.2 M-1 cm-1, ΦF= 2.3%). The …

Race/Ethnicity, Living Arrangements, And Access To Healthcare Among Older Adults With Alzheimer’S Disease In The United States: Multiple Systematic Reviews In Relation To Health Outcomes, Chela Reiss, Grace Vethacke

DePaul Discoveries

Alzheimer’s disease (AD) is a neurodegenerative condition primarily affecting older adults. It is a growing public health crisis that affects memory, thinking, and behavior. The number of adults aged 65 and older is projected to reach 98.2 million by 2060, leading to a continued rise in the prevalence of AD. Understanding the socioeconomic factors that influence health outcomes in patients with Alzheimer's Disease and Related Dementias (ADRD) is essential in addressing the disparities within patient care and quality of life. This systematic review examined three socioeconomic factors - race/ethnicity, living arrangements, and access to healthcare - and their related health …

Depaul Discoveries Volume 14 Cover Art, Reggina Moreno Trevino

DePaul Discoveries

No abstract provided.

Life Of Moo Deng, The Internet Sensation Pygmy Hippopotamus: An Overview Of The Biology And Conservation Breeding Program In Thailand, Ampika Thongphakdee, Atthapon Nundee, Umaporn Maikeaw, Narongwit Chodchoy, Chainarong Punkong, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

The hippopotamus, commonly known as the hippo, is classified into two extant species: the common hippopotamus (Hippopotamus amphibius) and the pygmy hippopotamus (Choeropsis liberiensis). Pygmy hippos are fascinating creatures native to the forests and swamps of West Africa. Their survival is increasingly threatened by habitat loss resulting from agricultural expansion and urban development. As the population declines, zoological institutions play a crucial role in conservation by acting as genetic reservoirs and promoting public awareness of wildlife and environmental protection. “Moo Deng”, a pygmy hippo, whose name in Thai has gained international recognition, represents a notable success …

Larger Price Limits For Cattle Futures Contracts: Should We Pay Attention To That?, Fabio Mattos

Cornhusker Economics

The CME Group has recently announced an increase in price limits for its cattle futures contracts. This means that cattle futures prices now have a wider range to fluctuate on any given day, and the possibility of larger price fluctuations implies that traders may find larger gains as well as larger losses in their trading accounts at the end of the day. This is certainly something that people trading in futures markets should be aware of, but the change in price limits itself is just a regular update that happens every year. Let's take a closer look at this.

Computational And Imaging Approaches For Precision Characterization Of Bone, Cartilage, And Synovial Biomolecules., Rahul Kumar, Kyle Sporn, Vibhav Prabhakar, Ahab Alnemri, Akshay Khanna, Phani Paladugu, Chirag Gowda, Louis Clarkson, Nasif Zaman, Alireza Tavakkoli

Department of Medicine Faculty Papers

Abstract

Background/Objectives: Degenerative joint diseases (DJDs) involve intricate molecular disruptions within bone, cartilage, and synovial tissues, often preceding overt radiographic changes. These tissues exhibit complex biomolecular architectures and their degeneration leads to microstructural disorganization and inflammation that are challenging to detect with conventional imaging techniques. This review aims to synthesize recent advances in imaging, computational modeling, and sequencing technologies that enable high-resolution, non-invasive characterization of joint tissue health. Methods: We examined advanced modalities including high-resolution MRI (e.g., T1ρ, sodium MRI), quantitative and dual-energy CT (qCT, DECT), and ultrasound elastography, integrating them with radiomics, deep learning, and multi-scale modeling approaches. We …

Hemp Cultivation Under Off-Season High Tunnel Growth Chamber Environments In South Louisiana, Gabriel Lococo

LSU Master's Theses

Industrial hemp (Cannabis sativa) production in Louisiana began in 2020 with 932 acres cultivated for fiber and cannabidiol (CBD) extraction. By 2024, production had declined to just 58.4 acres (Louisiana Department of Agriculture and Forestry, personal communication). During the 2020–2021 season, over 40% of field-grown hemp was lost due to tropical storms, insect pressure, and diseases associated with warm, wet conditions. To mitigate these challenges, hemp was planted and harvested between November and January, a cooler period with reduced environmental stress.

‘Sour Citron’, a day-neutral cultivar, was selected for its insensitivity to photoperiod, enabling flowering during shorter days. …

Human Contributions To Global Soundscapes Are Less Predictable Than The Acoustic Rhythms Of Wildlife, Panu Somervuo, Tomas Roslin, Brian L. Fisher, Bess Hardwick, Deirdre Kerdraon, Dimby Raharinjanahary, Eric Tsiriniaina Rajoelison, Patrik Lauha, Lukas Griem, Petteri Lehikoinen, Pekka Niittynen, Esko Piirainen, Markus Lumme, Ville-Matti Riihikoski, Orlando Acevedo-Charry, Solny A. Adalsteinsson, Maaz Ahmad, Sandra Alcobia, Jón Aldará, Tad Dallas

Faculty Publications

Across the world, human (anthropophonic) sounds add to sounds of biological (biophonic) and geophysical (geophonic) origin, with human contributions including both speech and technophony (sounds of technological devices). To characterize society’s contribution to the global soundscapes, we used passive acoustic recorders at 139 sites across 6 continents, sampling both urban green spaces and nearby pristine sites continuously for 3 years in a paired design. Recordings were characterized by bird species richness and by 14 complementary acoustic indices. By relating each index to seasonal, diurnal, climatic and anthropogenic factors, we show here that latitude, time of day and day of year …