Life Sciences Commons^™

Dynamics Of Mangrove Carbon Stock In Selected Uae Coastline: A Remote Sensing Approach, Karthika Kandamkulathil Gopi

Thesis/ Dissertation Defenses

Mangrove ecosystems play a vital role in carbon sequestration by capturing atmospheric CO₂, storing it in aboveground and belowground biomass, and enhancing soil organic carbon (SOC) through litterfall. Arid mangrove ecosystems, such as those in the UAE, experience extreme environmental stressors, leading to distinct variations in biomass allocation and carbon stock estimates compared to tropical regions. This study aims to evaluate biomass accumulation, litterfall production, and SOC storage in selected UAE mangroves. It analyses seasonal litterfall dynamics and assesses the influence of bulk density, soil moisture, and tidal regimes on SOC and aboveground biomass (AGB) across distinct mangrove zones. Field-based …

The Anticancer Activity Of Novel Chromene Derivatives Against Colorectal Cancer Cells, Rym Magramane

Thesis/ Dissertation Defenses

Colorectal cancer (CRC) stands as the third most common cancer globally, accounting for nearly 10% of all newly diagnosed cancer cases. In the UAE, CRC was ranked as the third most frequently diagnosed malignancy, and the leading cause of cancer-associated deaths in 2023. Standard CRC treatment strategies include surgical resection, chemotherapy, immunotherapy, targeted therapy, or a combination of all, depending on the cancer stage and molecular profile. Despite these interventions, disease recurrence is experienced by a considerable number of cancer patients. Hence, there is an urgent need to develop novel alternative treatments to overcome poor prognosis. Recently, cancer research has …

Associations Between 24-Hour Movement Compositions And Cardiometabolic Health In Children And Adolescents: A Five-Part Compositional Analysis Using Data From The International Children’S Accelerometery Database (Icad), Zoë A. Marshall, Adam Runacres, Pedro Curi Hallal, Russ Jago, Soyang Kwon, Kate Northstone, Russell R. Pate, Jardena Puder, John J. Reilly, Luis B. Sardinha, Niels Wedderkopp, Ester Van Slujis

Faculty Publications

Objectives The benefits of physical activity (PA) and the negative impacts of sedentary time (SED) on health in youth are well established. However, uncertainty remains surrounding how PA and SED jointly influence cardiometabolic risk (CMR) factors. The aim of this study was to determine the joint influence of SED, light-, moderate- and vigorous-intensity PA (LPA, MPA and VPA), and sleep on CMR factors using five-part compositional analyses.

Methods Data were pooled from 16 cohort studies comprising 22 474 children and adolescents from the International Children’s Accelerometery Database. PA was measured using hip-mounted accelerometers with sleep self-reported. CMRs included body mass …

Functional Characterization Of Uncharacterized Essential Genes In The Oral Pathogen Streptococcus Mutans, Courtney Dover

Student Theses and Dissertations

Streptococcus mutans is a pathogen associated with the formation of dental caries, and its genome contains many uncharacterized genes, including some which are classified as essential genes. These genes are required for the growth of the organism. Characterizing the function of these genes is important for understanding the organism, as well as identifying potential antimicrobial targets. This project used a CRISPRi (CRISPR interference) system to repress seven experimentally uncharacterized genes within the S. mutans genome and investigated the effects of their repression via RNA-seq transcriptomics, phylogenetic analysis, and TEM. The gene annotated as SMU_393 was then prioritized for further analysis …

Role Of Gut-Liver-Brain Axis In Neuropsychiatric Symptoms Of Parkinson’S Disease, Raveena Dhanani Ratnayake

Student Theses and Dissertations

Neuropsychiatric symptoms (NPS), such as depression, and anxiety, and mild cognitive impairment (MCI), often precede motor symptoms in Parkinson’s disease (PD), highlighting the need for early biomarkers and mechanistic insights. Gut-microbiome-related metabolites, including secondary bile acids, influence lipid homeostasis and mitochondrial function, impacting neuropsychiatric and neurodegenerative disorders. This study examines metabolic alterations in PD, focusing on lipid metabolism, bile acids, acylcarnitine, and amino acids. Our findings showed significant changes in lipid metabolism, transsulfuration pathway, bile acids, acylcarnitine, and amino acids in PD severity and NPS. Specific lipid subclasses were linked to NPS, cognitive impairment, and motor impairment. Given the limited …

Weather Impacts On Specialty Fruit Wholesale Prices, Oladayo Daniel Oluleye

LSU Master's Theses

This article investigates the relationship between temperature and precipitation variability and wholesale specialty fruit prices in the United States. Using fixed-effects panel regression and a comprehensive dataset of monthly price observations across ten major U.S. wholesale markets (Atlanta, Baltimore, Boston, Chicago, Detroit, Los Angeles, Miami, New York, Philadelphia and San Francisco) from 2000 to 2019, we examine how weather fluctuations affect wholesale market prices for five fruits including apples, avocados, grapes, oranges, and strawberries. Our analysis categorizes temperature into discrete bins representing the number of days per month within specific temperature ranges. The results show that extreme temperatures, particularly above …

Genetic Structuring Of The Ozark Pocket Gopher (Geomys Bursarius Ozarkensis) And Coevolution With Its Geomydoecus Ectoparasites, Monica Raquel Reusche

Student Theses and Dissertations

The Ozark pocket gopher Geomys bursarius ozarkensis is a highly isolated and restricted subspecies of the plains pocket gopher endemic to Izard Co., Arkansas. This isolation may have impacts genetically and may have caused speciation of their chewing louse. First, I updated the distribution and confirmed occupation at 46 previously documented and 91 additional sites, a 34% increase in areal range. Next, I evaluated natal dispersal which was rare (9.1%) and occurred over shorter distances (≤ 63 m) than for other gopher species. Analysis of single nucleotide polymorphisms from contemporary populations and museum specimens indicated a high genetic structure among …

Stepping Into Motherhood: Pregnancy-Induced Changes To Foot Structure And Biomechanics, Lauren R. Williams

Theses and Dissertations

INTRODUCTION: Pregnant women have an elevated risk of developing lower limb musculoskeletal disorders, likely due to the rapid increase in body mass and joint laxity. While a few studies have evaluated changes in static foot posture due to pregnancy, it is unclear if body mass or joint laxity is the primary driver. Additionally, how pregnancy affects the mechanics of the foot during dynamic movements is still not understood. PURPOSE: The purpose of this study is to investigate the foot's response to pregnancy. First, we examined foot tissue characteristics across pregnancy, specifically plantar fascia (PF) stiffness, length, and thickness. Additionally, we …

Yield And Pungency Of Eight Onion Varieties And Their Response To Sulfur Fertilizer On Fine-Textured Soil, Charlie Rohwer, Jesen Hosch, Brianna Hayward, Cindy Tong

Midwest Vegetable Trial Reports

Onions are notable for their pungency, caused by sulfur-containing compounds. Fine-textured soils high in organic matter, or soils fertilized with organic amendments, are unlikely to require additional sulfur for maximum onion yield. We were interested in the impacts of additional sulfur (in such a situation, unlikely to be sulfur-limited) on pungency, soluble solids, and yield in eight onion varieties. We carried out a study in 2024 in southern Minnesota, designed as a split-plot (onion variety as the whole-plot) with four replicates. Heavy rainfall caused substantial unmarketability in our trial, with ‘Ringmaster’ suffering the most loss (11% of planted ‘Ringmaster’ were …

Acute Effects Of Blood Flow Restriction During Low-Load Exercise And Recovery On Blood Flow And Performance, Shay B. Richards

Theses and Dissertations

Purpose: Use of blood flow restriction (BFR) during strength training with light loads has been used to induce similar muscular benefits as traditional strength training with heavy loads. Safe and effective recommendations for the use of BFR relies on an understanding of how different methods of BFR alter blood flow during rest and exercise. The purpose of this study was to compare blood flow during exercise and recovery between continuous BFR (CONT), intermittent BFR (INT), and no BFR (NBFR) as a control. Methods: 16 participants (8 male, 8 female) participated in this study on three different days, separated by at …

Common Aav Gene Therapy Vectors Show Nonselective Transduction Of Ex Vivo Human Brain Tissue, J P Mcginnis, Joshua Ortiz-Guzman, Maria Camila Guevara, Sai Mallannagari, Benjamin D W Belfort, Suyang Bao, Snigdha Srivastava, Maria Morkas, Emily Ji, Angela Addison, Evelyne K Tantry, Sarah Chen, Ying Wang, Zihong Chen, Kalman A Katlowitz, Jeffrey J Lange, Melissa M Blessing, Carrie A Mohila, M Cecilia Ljungberg, Guillermo Aldave, Ali Jalali, Akash Patel, Sameer A Sheth, Howard L Weiner, Shankar Gopinath, Ganesh Rao, Akdes Serin Harmanci, Daniel J Curry, Benjamin R Arenkiel

Children’s Nutrition Research Center Staff Publications

The ability to deliver a therapeutic sequence to a specific cell type in the human brain would make possible innumerable therapeutic options for some of our most challenging diseases; however, studies on adeno-associated virus (AAV) vector tropism have generally relied on animal models with limited translational utility. For this reason, establishing the tropism of common adeno-associated virus (AAV) vectors in living human brain tissue serves as an important baseline for further optimization, as well as a determination of human brain cell types transduced by clinically approved gene therapy vectors AAV2 and AAV9. We have adapted an

The Effect Of Functional Training On Dynamic Knee Valgus Of Youth Soccer Players. A Pilot Study, Ole Tovar, Dóra Molnár, Badis Soussi, Ádám Uhlár, Tamás Horváth, Mira Ambrus

International Journal of Exercise Science

International Journal of Exercise Science 18(7): 561-574, 2025. Knee injuries are very common in soccer players. High loads on the knee during landing or changes of direction can lead to a valgus shift of the knee, known as Dynamic Knee Valgus (DKV). Many studies have shown that a large shift in DKV is a predisposing factor for knee injuries and disease, such as anterior cruciate ligament injury (ACL), patellofemoral pain syndrome or osteoarthritis. Functional training could be a great tool to reduce DKV. Thus, in this pilot study, nine male youth soccer players (mean age: 16.4 ± 0.2 years) took …

Impact Of Nitrogen Fertilizer Application Rates On Plant Growth And Yield Of Organic Kale And Swiss Chard In Vertical Farming System, Andruw Jones, Sai Prakash Naroju, Dilip Nandwani, Anthony Witcher, Shahidullah Chowdhary

Agricultural and Environmental Sciences Faculty Research

To support the growing global population, sustainable farming methods like vertical farming must complement traditional agriculture. This study evaluated the effects of various nitrogen fertilizer application rates (N_low (1055.3 ppm), N_rec (1640.9 ppm), N_high (2811.3 ppm), and N_0 (469.9 ppm)) on organic kale (Brassica oleracea L. var. acephala ‘Lacinato’) and Swiss chard (Beta vulgaris subsp. Vulgaris ‘Ruby/Rhubarb Red’), grown in a vertical growing system installed in a high tunnel during the spring and fall season of 2023 at the organic farm of Tennessee State University. Growth parameters studied included fresh weight, Brix, chlorophyll, plant height, and leaf count. Most parameters …

Highly Adaptable Dendrimer Gel Nanoparticles With Dual Targeting Of Upar And Ribonucleotide Reductase R2 For Better Retention And Improved Therapeutic Outcomes In Triple-Negative Breast Cancer, Hsin Yin Chuang, Da Huang, Lin Qi, Vidit Singh, Anna Chernatynskaya, Yue-Wern Huang, Hu Yang

Biological Sciences Faculty Research & Creative Works

Triple-negative breast cancer (TNBC) accounts for approximately 15% of breast cancers and lacks estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), rendering it unresponsive to hormonal or anti-HER2 therapies. Due to its poor prognosis and limited treatment options, there is an urgent need for targeted therapies. In this study, we developed highly adaptable polyamidoamine (PAMAM) dendrimer-based gel nanoparticles with dual-targeting capabilities against urokinase-type plasminogen activator receptor (uPAR) and ribonucleotide reductase R2 (R2). These nanoparticles were designed to target both TNBC cells and cancer-associated stromal cells by leveraging uPA-uPAR interactions and delivering the antisense oligonucleotide …

Assessment Of Walkability Of University Campus And Communities: Associations With Perceived Safety & Student Retention, Wendy Bjerke

Exercise Science Faculty Publications

Introduction: Approximately 80% of Americans do not meet minimum guidelines for regular physical activity. Among ways to promote physical activity is to optimize the Built Environment to promote and increase access to walking and cycling. Walkable and accessible university campus environments and proximal communities can promote physical activity and are assessed and compared in this study. Purpose and Methods: Approximately 20 million students attend colleges and universities in the United States with over 100,000 students enrolled in the state of Connecticut. These students can potentially engage in physical activity within a campus or around the surrounding communities for university related …

Asset Or Liability? Farmers’ Perceptions Of Improved Maize Seeds As A Climate Change Adaptation Strategy In Semi-Arid Areas Of Chamwino District, Tanzania, Jackson Raymond Sawe

Journal of Humanities and Social Sciences

This study explores the perceptions of smallholder farmers in semi-arid areas of Chamwino District, Tanzania, regarding the adoption of improved maize seeds as an adaptation strategy to climate change. It aims to explore whether farmers view the use of these seeds as an asset that helps them adapt to climate change, or as a liability that presents challenges resulting from the adoption of improved seeds. The study employs both quantitative and qualitative methods for data collection and analysis. Data were collected from 125 smallholder farmers across six study villages through household surveys, in-depth interviews, field observation, and document review. Participants …

Disentangling The Channels Of Impact Of The Ukraine War On Global Food Markets: An Integrated Scenario Approach, Maksym Chepeliev, Maryla Maliszewska, Maria Filipa Seara E Pereira

Department of Agricultural Economics Faculty Publications

The war in Ukraine has major implications for the world's agricultural and food markets, as the Black Sea region is a large supplier of crops and fertilizers. Impacts of the war are further exacerbated by the sanctions against Russia, spillover effects from disruptions of other commodity markets, adverse weather events and domestic policies that countries around the world have implemented in pursuit of food security. In this paper, using a comprehensive modeling framework, we decompose the impacts across various channels and show that the cumulative effect of the considered indirect spillovers is even more substantial than the direct agricultural supply …

Rethinking Large-Scale Phylogenomics With Eukphylo V.1.0, A Flexible Toolkit To Enable Phylogeny-Informed Data Curation And Analyses Of Diverse Eukaryotic Lineages, Laura A. Katz, Marie Leleu, Godwin Ani, Rebecca Gawron, Auden Cote-L'Heureux

Biological Sciences: Faculty Publications

Eukaryotic diversity is largely microbial, with macroscopic lineages (plants, animals, and fungi) nesting among a plethora of diverse protists. Our understanding of the evolutionary relationships among eukaryotes is rapidly advancing through ’omics analyses, but phylogenomic analyses are challenging for microeukaryotes, particularly uncultivable lineages, as single-cell sequencing approaches generate a mixture of sequences from hosts, associated microbiomes, and contaminants. Moreover, many analyses of eukaryotic gene families and phylogenies rely on boutique data sets and methods that are challenging for other research groups to replicate. To address these challenges, we present EukPhylo v.1.0, a modular, user-friendly pipeline that enables effective data curation …

Electric Weed Control—How Does It Compare To Conventional Weed Control Methods?, Catherine Borger, Miranda J. Slaven

Journal articles

Weed management practices in agroecosystems mainly rely on herbicide, mowing, or tillage. Electric weed control offers a novel alternative, with a range of commercially available products for weed management in agricultural environments. However, electrical weed control efficacy has not been effectively compared with conventional weed management practices. Further, electrical weed control products may have a fire risk, as highlighted but not assessed in prior studies. The current study evaluated an electric weed control machine (Zasso™ XPower) for weed management in four vineyard sites (in 2022 and 2023) in comparison to mowing and herbicide applications. Weed control tactics were applied in …

Multigenerational Exposure To Glyphosate Has Only Modest Effects On Life-History Traits, Stress Tolerance And Microbiome In A Field Cricket, J. W. Whitlock, P. M. Orwin, Z. R. Stahlschmidt

Pacific Faculty Work

Glyphosate is the most used herbicide worldwide, and it can be toxic to off-target species, such as insects. Although GLY-based herbicides (GBHs) can influence insect microbiomes, little is known about its cascading effects on fitness-related traits, such as life history or stress tolerance, especially in the context of long-term, multigenerational exposure. Thus, we exposed the variable field cricket, Gryllus lineaticeps, to GBH within and across generations to examine the potential role of GBH in developmental plasticity and evolution. Specifically, we measured its effects on life-history traits (e.g. developmental duration, adult body size and mass, and a life-history trade-off between investment …

Citrullinated Eno1 Vaccine Enhances Pd-1 Blockade In Mice Implanted With Murine Triple-Negative Breast Cancer Cells, Ricardo A León-Letelier, Alejandro M Sevillano-Mantas, Yihui Chen, Soyoung Park, Jody Vykoukal, Johannes F Fahrmann, Edwin J Ostrin, Candace Garrett, Rongzhang Dou, Yining Cai, Fu-Chung Hsiao, Jennifer B Dennison, Eduardo Vilar, Banu K Arun, Samir Hanash, Hiroyuki Katayama

Faculty, Staff and Student Publications

Background/Objectives:Cancer vaccine targets mostly include mutations and overexpressed proteins. However, cancer-associated post-translational modifications (PTMs) may also induce immune responses. Previously, our group established the enzyme protein arginine deiminase type-2 (PADI2), which catalyzes citrullination modification, is highly expressed in triple-negative breast cancer (TNBC), promoting antigenicity.

Methods: Here, we show the workflow of designing citrullinated enolase 1 (citENO1) vaccine peptides identified from breast cancer cells by mass spectrometry and demonstrate TNBC vaccine efficacy in the mouse model. Immunized mice with citENO1 peptides or the corresponding unmodified peptides, plus Poly I:C as an adjuvant, were orthotopically implanted with a TNBC murine …

Proteomic-Based Stemness Score Measures Oncogenic Dedifferentiation And Enables The Identification Of Druggable Targets, Iga Kołodziejczak-Guglas, Renan L S Simões, Emerson De Souza Santos, Elizabeth G Demicco, Rossana N Lazcano Segura, Weiping Ma, Pei Wang, Yifat Geffen, Erik Storrs, Francesca Petralia, Antonio Colaprico, Felipe Da Veiga Leprevost, Pietro Pugliese, Michele Ceccarelli, Houtan Noushmehr, Alexey I Nesvizhskii, Bożena Kamińska, Waldemar Priebe, Jan Lubiński, Bing Zhang, Alexander J Lazar, Paweł Kurzawa, Mehdi Mesri, Ana I Robles, Clinical Proteomic Tumor Analysis Consortium, Li Ding, Tathiane M Malta, Maciej Wiznerowicz

Faculty, Staff and Student Publications

Cancer progression and therapeutic resistance are closely linked to a stemness phenotype. Here, we introduce a protein-expression-based stemness index (PROTsi) to evaluate oncogenic dedifferentiation in relation to histopathology, molecular features, and clinical outcomes. Utilizing datasets from the Clinical Proteomic Tumor Analysis Consortium across 11 tumor types, we validate PROTsi's effectiveness in accurately quantifying stem-like features. Through integration of PROTsi with multi-omics, including protein post-translational modifications, we identify molecular features associated with stemness and proteins that act as active nodes within transcriptional networks, driving tumor aggressiveness. Proteins highly correlated with stemness were identified as potential drug targets, both shared and tumor …

Foreign Aid, E. Wesley F. Peterson

Cornhusker Economics

According to a recent poll, United States adults think on average that about 26 percent of the federal government’s budget goes to foreign aid with some believing that more than half is put to that use. Public opinion surveys have returned similar results for many years with most respondents seeming to believe that this is far too generous often suggesting that something on the order of 5 percent of federal spending would be more appropriate. In fact, until recent efforts to eliminate foreign aid altogether, the actual amounts spent have represented one percent or less of the federal budget. The …

Morphological And Physiological Responses Of Cymbopogon Citratus And Pennisetum Alopecuroides To Saline Water Irrigation, Haifeng Xing, Asmita Paudel, Julie Hershkowitz, Youping Sun

Plants, Soils, and Climate Faculty Publications

Reclaimed water provides a sustainable and economical alternative for landscape irrigation, but its elevated salinity can negatively impact sensitive plant species. This study evaluated the salinity tolerance of two widely used ornamental grasses, Cymbopogon citratus (lemon grass) and Pennisetum alopecuroides (fountain grass), under three electrical conductivity (EC) levels: 1.2 (control), 5.0, and 10.0 dS·m⁻¹. Visual assessments over 62 days showed that both species maintained an acceptable appearance under saline conditions. C. citratus exhibited no foliar damage, with visual scores above 4.6 even at 10.0 dS·m⁻¹, whereas P. alopecuroides showed slight leaf injury but retained a score …

Anthropogenic Litter In Freshwater Ecosystems: Effect On Ecosystem Processes And Spatial Distribution, Emily Johnson

Master's Theses

The rapid increase of plastic production and disposal into the environment has resulted in anthropogenic litter (i.e., trash; AL) becoming a global problem. In aquatic ecosystems, macro- and microplastics are long-lived, mobile, and interact with the physical and chemical components of aquatic ecosystems. Our objectives were to: (1) assess the impact of microplastic pollution on the ecosystem processes of ecosystem metabolism and N2 flux in an oligotrophic lake and (2) to assess the abundance and assemblage of anthropogenic litter in a large urban river. For objective (1), we added microplastics at a range of concentrations to littoral mesocosms. Ecosystem metabolism …

Why Acromegaly Dies: A Systematic Review, Isam Noori Salman, Safaa Ehssan Atta, Baydaa Ahmed Abed, Noor Ulhuda G. Mohammed

Maaen Journal for Medical Sciences

Acromegaly is a rare endocrine clinical syndrome characterized by long-term elevated production of growth hormone (GH) due to a pituitary adenoma tumor. The main problem of acromegaly patients (AC-PTs) is prolonged elevated GH concentration, which leads to increased insulin-like growth factor 1 (IGF-1) production, causing the characteristic tissue and bone overgrowth. The appearance of diabetes in AC-PTs was linked with the high risks of cardiovascular morbidity, indicating that individuals with both conditions face heightened health challenges. Acromegaly typically has a higher cancer incidence rather than the general population. Without early diagnosis and effective treatment, excess GH can have widespread systemic …

High-Salt Tumor Microenvironment: Not As Bad As It Sounds, Not As Good As It Seems, Umer Ali, Venkataswarup Tiriveedhi

Biology Faculty Research

Recent evidence suggests a high-sodium microenvironment in breast tumors. However, the exact role of this high-sodium microenvironment on tumorigenesis is unknown. Salt (sodium chloride, NaCl) is a well-known inflammatory molecule playing a significant role in various chronic ailments like cardiovascular and autoimmune diseases. Importantly, chronic inflammation is recognized as one of the major hallmarks of carcinogenesis. Breast cancer cell culture-based studies demonstrated that high-salt (HS) treatment (Δ35–50 mM NaCl) induced cancer cell proliferation. However, preclinical murine research showed reduced tumor progression kinetics in mice fed a short-term HS diet (4% NaCl diet, 0–2 weeks prior to the injection of tumor …

Human Gut Commensal Alistipes Timonensis Modulates The Host Lipidome And Delivers Anti-Inflammatory Outer Membrane Vesicles To Suppress Colitis In An Il10-Deficient Mouse Model, Ethan Older, Mary K. Mitchell, Andrew Campbell, Xiaoying Lian, Michael Madden, Yuzhen Wang, Lauren E. Van De Wal, Thelma Zaw, Brandon N. Vanderveen, Rodney Tatum, E. Angela Murphy, Yan-Hua Chen, Daping Fang, Melissa Ellermann, Jie Li

Faculty Publications

Correlative studies have linked human gut microbes to specific health conditions. Alistipes is one such microbial genus negatively linked to inflammatory bowel disease (IBD). However, the protective role of Alistipes in IBD is understudied, and the underlying molecular mechanisms remain unknown. In this study, colonization of Il10-deficient mice with Alistipes timonensis DSM 27924 delays colitis development. Colonization does not significantly alter the gut microbiome composition, but instead shifts the host plasma lipidome, increasing phosphatidic acids while decreasing triglycerides. Outer membrane vesicles (OMVs) derived from Alistipes are detected in the plasma of colonized mice, carrying potentially immunomodulatory metabolites into the …

Insights Into The Floral Senescence Effects Of Nanoparticles, Vuk Uskoković

Administration and Staff Articles and Research

Flowers could be valuable addenda to nanomedicine labs as in vivo models crossing the gap between the cell culture and small mammals. However, as it is shown here, floral models continue to be predominantly utilized within resource-limited research environments and remain largely underrepresented in mainstream biomedical investigations conducted by well-funded institutions in the developed world. The ability to delay the senescence of Orange Jubilee wildflowers was tested here on three types of nanoparticles: selenite-doped hydroxyapatite (HAp), citrate-stabilized HAp, and superparamagnetic iron oxide. Integration of selenite into the HAp crystal lattice produced a mild increase in the senescence rate …

Optimized Genomic Editing Of A Common Duchenne Muscular Dystrophy Mutation In Patient-Derived Muscle Cells And A New Humanized Mouse Model, Mateusz Z Durbacz, Yu Zhang, Hui Li, Takahiko Nishiyama, Efrain Sanchez- Ortiz, John R Mcanally, Damir Alzhanov, Ning Liu, Eric N Olson

Faculty, Staff and Student Publications

Duchenne muscular dystrophy (DMD) is a fatal X-linked, recessive disease caused by mutations in the DMD gene encoding dystrophin, a membrane-associated protein necessary for maintaining muscle structure and function. One of the common DMD mutations is the deletion of exon 52 (Δ52), which introduces a premature stop codon in exon 53, preventing the expression of functional dystrophin protein. Patients with this mutation could benefit from skipping or reframing exon 53 to restore the dystrophin open reading frame. In this study, we investigated the efficacy of single-cut CRISPR gene editing with Staphylococcus pyogenes Cas9 (SpCas9)-LRVQR to restore dystrophin expression …