The Mucosal Expression Pattern Of Interferon-Ε In Rhesus Macaques, 2014 University of Nebraska-Lincoln
The Mucosal Expression Pattern Of Interferon-Ε In Rhesus Macaques, Andrew Demers, Guobin Kang, Fangrui Ma, Wuxun Lu, Zhe Yuan, Yue Li, Mark Lewis, Edmundo N. Kraiselburd, Luis Montaner, Qingsheng Li
Qingsheng Li Publications
Type I IFNs play an important role in innate and adaptive immunity against viral infections. A novel type I IFN, namely IFN-ε, which can protect against vaginal transmission of HSV2 and Chlamydia muridarum bacterial infection, has been described in mice and humans. Nevertheless, the principle cell type and the expression pattern of IFN-ε in tissues remain uncertain. In addition, the expression of IFN-ε in Indian rhesus macaques (Macaca mulatta) has not been reported. Here, we analyzed IFN-ε expression in multiple mucosal sites of uninfected or SIV-infected Indian rhesus macaques using IHCS. We report for the first time the detection of ...
Cryptorchidism And Infertility In Rats With Targeted Disruption Of The Adamts16 Locus, 2014 University of Toledo
Cryptorchidism And Infertility In Rats With Targeted Disruption Of The Adamts16 Locus, Shakila Abdul-Majeed, Blair Mell, Surya M. Nauli, Bina Joe
Pharmacy Faculty Articles and Research
A Disintegrin And Metalloproteinase with ThromboSpondin motifs16 (ADAMTS-16) is a member of a family of metalloproteinases. Using a novel zinc-finger nuclease based gene-edited rat model harboring a targeted mutation of the Adamts16 locus, we previously reported this gene to be linked to blood pressure regulation. Here we document our observation with this model that Adamts16 is essential for normal development of the testis. Absence of Adamts16 in the homozygous Adamts16(mutant) males resulted in cryptorchidism and male sterility. Heterozygous Adamts16(mutant) males were normal, indicating that this is a recessive trait. Testes of homozygous Adamts16(mutant) males were significantly smaller ...
Physiologic Assessment Of Blood Glucose Homeostasis Via Combined Intravenous Glucose And Insulin Testing In Horses, 2014 University of Tennessee, Knoxville
Physiologic Assessment Of Blood Glucose Homeostasis Via Combined Intravenous Glucose And Insulin Testing In Horses, Hugo Eiler, Nicholas Frank, Frank Andrews, Jack Oliver, Kellie Fecteau
Objective - To characterize the physiologic response to IV bolus injection of glucose and insulin for development of a combined glucose-insulin test (CGIT) in horses. Animals - 6 healthy mares and 1 mare each with pituitary adenoma and urolithiasis. Procedure - Horses were given a CGIT (glucose, 150 mg/kg; insulin, 0.1 U/kg); results were compared with a singular IV glucose tolerance test (GTT; 150 mg/kg) and a singular IV insulin sensitivity test (IST; 0.1 U/kg). Healthy horses were also given a CGIT after receiving xylazine and undergoing stress. Results - Physiologically, the CGIT resulted in a 2-phase curve ...
Inhibition Of Collagenase Breakdown Of Equine Corneas By Tetanus Antitoxin, Equine Serum And Acetylcysteine, 2014 University of Tennessee, Knoxville
Inhibition Of Collagenase Breakdown Of Equine Corneas By Tetanus Antitoxin, Equine Serum And Acetylcysteine, Kellie Fecteau, J Haffner, Hugo Eiler
OBJECTIVE: To determine whether tetanus antitoxin, equine serum, and acetylcysteine, which are currently used in the treatment of equine corneal ulcer, inhibit the digestion of equine corneal collagen when exposed to collagenase in vitro. ANIMALS STUDIED: Corneas from 40 adult horses. PROCEDURES: Sections of equine corneas were incubated with saline, a solution of bacterial collagenase in saline, bacterial collagenase in saline plus equine tetanus antitoxin, bacterial collagenase in saline plus equine serum, or bacterial collagenase in saline plus acetylcysteine. Each one of the collagenase inhibitors was tested at different concentrations. The degree of corneal collagen digestion was determined by concentrations ...
The Potential Of Collagenase As A New Therapy For Separation Of Human Retained Placenta: Hydrolytic Potency On Human, Equine And Bovine Placentae, 2014 University of Tennessee, Knoxville
The Potential Of Collagenase As A New Therapy For Separation Of Human Retained Placenta: Hydrolytic Potency On Human, Equine And Bovine Placentae, Kellie Fecteau, Hugo Eiler
The purpose of this study was to determine to what degree bacterial collagenase may digest human placentae compared to equine and bovine placentae. Placenta samples from human, equine and bovine were incubated with bacterial collagenase solution at various concentrations. The degree of hydrolysis and collagen breakdown was measured by the release of total proteins and hydroxyproline into the incubation media. Also, whole placentae were injected via umbilical cord arteries with collagenase solution (200 U/ml, 200 ml total volume in human and 1000 ml in equine) and hydrolysis determined chemically and subjectively. Human and equine placental collagens were the most ...
Placenta Detachment: Unexpected High Concentrations Of 5-Hydroxytryptamine (Serotonin) In Fetal Blood And Its Mitogenic Effect On Placental Cells In Bovine, 2014 University of Tennessee, Knoxville
Placenta Detachment: Unexpected High Concentrations Of 5-Hydroxytryptamine (Serotonin) In Fetal Blood And Its Mitogenic Effect On Placental Cells In Bovine, Kellie Fecteau, Hugo Eiler
The purpose of this study was twofold: (1) to determine whether there is a profile of 5-hydroxytryptamine (5-HT, serotonin) concentrations in fetal bovine blood and tissues during pregnancy, parturition and the early neonatal period and (2) to determine whether 5-HT has a 'mitogenic' effect on cultured placentome cells in bovine. Results revealed a 5-HT concentration profile in fetal blood. Overall concentrations of 5-HT in fetal blood were 6.6 times (P< 0.001) that of adult cows and 2.8 times (P< 0.001) that of blood collected during caesarean section (from umbilical veins) and from 18-72 h old calves ...
Retained Placenta, 2014 University of Tennessee, Knoxville
Retained Placenta, Hugo Eiler, Kellie Fecteau
No abstract provided.
Prevention Of Retained Placenta By Injection Of Collagenase Into Umbilical Arteries Of Calves Delivered By Cesarean Section: A Tolerance Study, 2014 University of Tennessee, Knoxville
Prevention Of Retained Placenta By Injection Of Collagenase Into Umbilical Arteries Of Calves Delivered By Cesarean Section: A Tolerance Study, Hugo Eiler, P Wan, N Valk, Kellie Fecteau
In the cow, cesarean section delivery is often followed by retention of fetal membranes. Hypothetically, the retention of fetal membranes could be prevented by intraplacental injections of the enzyme collagenase. However, the infusion of this potent proteolytic enzyme into a uterus traumatized by surgery can lead to uterine damage, including perforation. Thus, the objective of this research was to evaluate tolerance of intraplacental treatment of bacterial collagenase. A cesarean section was performed on 10 experimental cows undergoing induced delivery or diagnosed with dystocia. During the surgical procedure, 200,000 units of bacterial collagenase in 1 L of saline were infused ...
Associations Of Adipose Tissue Architecture, Adipokines And Inflammatory Markers With Body Mass Index And Gestational Weight Gain In Non-Diabetic Pregnancies, 2014 University of Massachusetts Medical School
Associations Of Adipose Tissue Architecture, Adipokines And Inflammatory Markers With Body Mass Index And Gestational Weight Gain In Non-Diabetic Pregnancies, Tiffany A. Moore Simas, Crina Boeras, Ningning Zhang, Olga Gaelikman, Katherine G. Leung, Nicole M. Wedick, Laura Robidoux, Jodi Adams Puleo, Mary M. Lee, Silvia Corvera, Milagros C. Rosal
UMass Center for Clinical and Translational Science Research Retreat
Background: Some pregnancy weight gain is stored as adipose tissue (AT). Human AT depots vary in their capacity for expansion. Data suggests that subcutaneous (SQ) is adapted for healthy lipid storage. Conversely visceral (V) accumulation is associated with inflammation, obesity-related co-morbidities and Type 2 diabetes (T2DM) risk. We investigated SQ and VAT histologic architecture along with insulin, adipokines and inflammatory markers in relationship to prepregnancy BMI and gestational weight gain (GWG).
Methods: Subset of non-diabetic singleton gravidas from the Pregnancy & Postpartum Observational Dietary Study (PPODS), undergoing Cesareans and consenting to SQ & VAT biopsies were included. Average adipocyte size assessed in10 sections/depot/subject. Maternal and cord blood insulin, adiponectin, leptin, PAI-1, CRP, TNFα, IL1b, IL6 and IL8 evaluated using Luminex MAGPIX, laser based fluorescent analytical test instrumentation with MILLIPLEX® multi-analyte panels. GWG determined by difference in pre-pregnancy and last prenatal visit weight.
Results: Of 110 subjects enrolled, 19 (17.3%) delivered by Cesarean with 14 consenting to AT sampling, and 7 (50%) having both SQ and VAT available for analysis. These 7 had mean pre-pregnancy BMI 27.8±5.6 kg/m2 and GWG 50.0±25.7 lb (range 19-83) with delivery age 39.2±0.7 wks. Mean SQ and VAT adipocyte sizes were 2892±716 pixels2 (range 1866-3775) and 2427±641 pixels2 (range 1416-3397) respectively (p=0.310); neither were statistically correlated with BMI or GWG. Pre-pregnancy BMI statistically correlated with maternal serum insulin (0.786, p=0 ...
Adipose Tissue Inflammation In Diabetic Versus Non-Diabetic Pregnancies, 2014 University of Massachusetts Medical School
Adipose Tissue Inflammation In Diabetic Versus Non-Diabetic Pregnancies, Tiffany A. Moore Simas, Raziel Rojas-Rodriguez, Jacqueline Tessa Draper, Aylin Sert, Maxwell Kruse, Crina Boeras, Brittany Rosenthal, Olga Gaelikman, So-Yun Min, Katherine G. Leung, Silvia Corvera
UMass Center for Clinical and Translational Science Research Retreat
Objective: Beyond weight associated with fetus, placenta, amniotic fluid and increased blood volume, adipose tissue (AT) expansion is an accepted and expected component of pregnancy weight gain. Normal pregnancy is associated with relative insulin resistance (IR). In non-pregnant humans, AT expansion has been associated with IR and AT inflammation. However, it is not known whether AT expansion and IR in pregnancy are also associated with AT inflammation. This pilot study examined relationships between AT expansion and inflammation in control versus diabetic pregnancies.
Methods: Eligible subjects undergoing scheduled Cesarean delivery for obstetric indications were prospectively enrolled. Subjects provided demographic and anthropometric ...
Paternal Poly (Adp-Ribose) Metabolism Modulates Retention Of Inheritable Sperm Histones And Early Embryonic Gene Expression, 2014 University of Pennsylvania
Paternal Poly (Adp-Ribose) Metabolism Modulates Retention Of Inheritable Sperm Histones And Early Embryonic Gene Expression, Motomasa Ihara, Mirella L. Meyer-Ficca, N. A. Leu, Shilpa Rao, Fan Li, Brian D. Gregory, Irina A. Zalenskaya, Richard M. Schultz, Ralph G. Meyer
Departmental Papers (Biology)
To achieve the extreme nuclear condensation necessary for sperm function, most histones are replaced with protamines during spermiogenesis in mammals. Mature sperm retain only a small fraction of nucleosomes, which are, in part, enriched on gene regulatory sequences, and recent findings suggest that these retained histones provide epigenetic information that regulates expression of a subset of genes involved in embryo development after fertilization. We addressed this tantalizing hypothesis by analyzing two mouse models exhibiting abnormal histone positioning in mature sperm due to impaired poly(ADP-ribose) (PAR) metabolism during spermiogenesis and identified altered sperm histone retention in specific gene loci genome-wide ...
Maternal Biomarkers Of Endothelial Dysfunction And Preterm Delivery, 2014 Rowan University
Maternal Biomarkers Of Endothelial Dysfunction And Preterm Delivery, Xinhua Chen, Theresa O Scholl
School of Osteopathic Medicine Faculty Scholarship
BACKGROUND: Endothelial dysfunction is key to the development of atherosclerosis. Preterm delivery foreshadows later maternal cardiovascular disease (CVD), but it is not known if endothelial dysfunction also occurs. We prospectively measured circulating biomarkers of endothelial dysfunction in pregnant women with preterm or term delivery.
METHODS: We conducted a case-control study nested within a large prospective epidemiological study of young, generally healthy pregnant women. Women who delivered preterm (gestation, n = 240) and controls who delivered at term (n = 439) were included. Pregnancies complicated by preeclampsia were analyzed separately. Circulating endothelial dysfunction biomarkers included soluble intercellular adhesion molecule-1 (sICAM-1), vascular cell adhesion ...
From Neurodegeneration To Infertility And Back - Exploring Functions Of Two Genes: Armc4 And Tardbp: A Dissertation, 2014 University of Massachusetts Medical School Worcester
From Neurodegeneration To Infertility And Back - Exploring Functions Of Two Genes: Armc4 And Tardbp: A Dissertation, Wei Cheng
GSBS Dissertations and Theses
Amyotrophic Lateral Sclerosis (ALS) is an adult-onset progressive neurodegenerative disease that causes degeneration in both upper and lower motor neurons. ALS progresses relentlessly after the onset of the disease, with most patients die within 3-5 years of diagnosis, largely due to respiratory failure. Since SOD1 became the first gene whose mutations were associated with ALS in 1993, more than 17 ALS causative genes have been identified. Among them, TAR DNA-binding protein (TARDBP) lies in the central of ALS pathology mechanism study, because TDP43 proteinopathy is observed not only in familial ALS cases carrying TARDBP mutations, but also in most of ...
Cellular And Molecular Mechanisms Of Chemical-Induced Ovarian Toxicity, 2014 Iowa State University
Cellular And Molecular Mechanisms Of Chemical-Induced Ovarian Toxicity, Shanthi Ganesan
Graduate Theses and Dissertations
The ovary is the ovum-producing female reproductive organ composed of follicles at different stages of development. Chemicals which selectively damage large growing or antral follicles only temporarily interrupt reproductive function because these follicles can be replaced by recruitment from the greater pool of primordial follicles. However, chemicals that destroy oocytes contained in primordial follicles can cause permanent infertility and premature ovarian failure (early menopause in women), since once a primordial follicle is destroyed, it cannot be replaced. We hypothesized that ovotoxic chemicals can bind to DNA to induce DNA damage and that the ovarian cells activate DNA repair, cell cycle ...
Anti-Müllerian Hormone In Stallions And Mares: Physiological Variations, Clinical Applications, And Molecular Aspects, Anthony N.J. Claes
Theses and Dissertations--Veterinary Science
Anti-Müllerian hormone (AMH) is a homodimeric glycoprotein that is best known for its role in regression of the Müllerian duct in the male fetus. Accumulating evidence indicates that AMH also has an important role during different physiological processes after birth. In contrast to other species, relatively little is known about AMH in the horse. In chapter one, developmental and seasonal changes in serum AMH concentrations in male horses were determined, and the use of AMH for determination of retained cryptorchid testes was established. In chapter two, the interrelationship between plasma AMH concentrations, antral follicle counts (AFC), and age in mares ...
Jordanian Ethical Perceptions Of Preimplantation Genetic Diagnosis, 2013 SIT Study Abroad
Jordanian Ethical Perceptions Of Preimplantation Genetic Diagnosis, Sarah C. Miller
Independent Study Project (ISP) Collection
Preimplantation Genetic Diagnosis (PGD) is a procedure that can be done during In Vitro Fertilization (IVF), involving genetic and chromosomal screening of fertilized embryos prior to implantation into a woman’s uterus. After PGD, only the embryos possessing the desired characteristics are implanted, while the other embryos are frozen for future use, or discarded. PGD is used for family balancing (gender selection for balancing the gender dynamic within a family), gender selection to screen out sex-linked diseases, or a broader genetic screening for diseases known to be present in the patient’s family history.
Many ethical questions have been raised ...
Chemotherapy-Induced Premature Menopause Among Latina Women With Breast Cancer: An Interpretive Description: A Dissertation, 2013 University of Massachusetts Medical School Worcester
Chemotherapy-Induced Premature Menopause Among Latina Women With Breast Cancer: An Interpretive Description: A Dissertation, Maryellen D. Brisbois
Graduate School of Nursing Dissertations
The description and interpretation of Latinas’ experience with chemotherapyinduced premature menopause from breast cancer treatment were explored in this study, which utilized an interpretive descriptive method from a feminist lens, and Knobf’s (1998, 2002) “Carrying on” theory. The specific aims of the study and the interview questions were guided by the state of the science literature. Overall, the impact of physiological effects, psychosocial effects, barriers, influencing factors that made their experience easier or harder, and how participants adjusted to a cancer diagnosis, treatment course, and menopause transition were described as bigger than the menopause experience alone. Participants also described ...
Kisspeptin Receptor Agonist (Ftm080) Increased Plasma Concentrations Of Luteinizing Hormone In Anestrous Ewes, 2013 University of Tennessee - Knoxville
Kisspeptin Receptor Agonist (Ftm080) Increased Plasma Concentrations Of Luteinizing Hormone In Anestrous Ewes, Brian K. Whitlock, Kelly A. Chameroy, Rebecca R. Payton, Shinya Oishi, Nobutaka Fujii, Joseph A. Daniel
Brian K Whitlock, PhD, DVM, DACT
Kisspeptin (KP) and the KP receptor (Kiss1r) are integral to central regulation of the gonadotropic-axis. The demonstration that intravenous infusion of KP can stimulate gonadotropin secretion and ovulation in seasonally anestrous female sheep offers a means of manipulating the reproductive axis. However, KP may be of limited clinical use because of the short circulating half-life. Kiss1r agonists with increased half-life and similar efficacy to KP in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test and compare the effects of ...
Coexistence Of Postpartum Cardiomyopathy And Single Coronary Artery Anomaly, 2013 Universal Hospitals Group Obstetrics and Gynaecology, MALATYA
Coexistence Of Postpartum Cardiomyopathy And Single Coronary Artery Anomaly, Murat Bozkurt, Gonca Gül Boz, Ahmet Uğur Boz, Duygu Kara
Proceedings in Obstetrics and Gynecology
Postpartum cardiomyopathy (PPCM) is dilated cardiomyopathy seen in the postpartum period in women who no prior history of cardiac diseases. In this case study, coronary angiography was used to determine the etiology of the cardiomyopathy after the decompensated heart failure had regressed. A congenital single coronary artery anomaly was detected by coronary angiography. In this case, PPCM can mimic pneumonia, thus making diagnosis difficult. When there is no response to antibiotic therapy, the obstetrician must consider cardiac disease and appropriate consultation should be obtained as soon as possible. To the best of our knowledge, single coronary artery anomalies coexisting with ...
Meloxicam Mediates Short-Term Behavioral Changes Of Castrated Calves, 2013 University of Tennessee - Knoxville
Meloxicam Mediates Short-Term Behavioral Changes Of Castrated Calves, Peter D. Krawczel, Jeff A. Carroll, Nicole C. Burdick Sanchez, Jeff W. Dailey, Joseph A. Daniel, Johann F. Coetzee, Brian K. Whitlock
Brian K Whitlock, PhD, DVM, DACT
Castration may detrimentally affect the health and performance of weaned calves and painful procedures are increasingly a public concern. Therefore, practical pain mitigation is critical. The objective was to determine the effects of castration (by banding) with or without administration of NSAID, meloxicam, on the behavior of weaned beef calves. Forty-eight (56 d post-weaning) beef calves [10.0 ± 0.2 (mean ± SE) mo old; 304 ± 6 kg BW] were blocked by multiple factors then randomly assigned to 3 treatments (n = 16 calves per treatment: 1) intact bulls (BULL), 2) castration by banding (BAN), or 3) castration by banding with orally-administered ...