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Investigation Of Phanerochaete Chrysosporium And Clostridium Thermocellum For Improved Saccharification Of Lignocellulose Under Nonsterile Conditions, William E. Simon 2015 University of Kentucky

Investigation Of Phanerochaete Chrysosporium And Clostridium Thermocellum For Improved Saccharification Of Lignocellulose Under Nonsterile Conditions, William E. Simon

Theses and Dissertations--Biosystems and Agricultural Engineering

Current research efforts are directed at developing competitive processes that can utilize lignocellulose as a feedstock for biorefineries. The purpose of this study was to investigate methods of processing lignocellulosic material so that its monosacharides can be more easily accessed for fermentation, the lack of which is hindering the economics and widescale adoption of lignocellulosic biorefining. The monosaccharides are of interest because they can be used by Clostridium beijerinckii downstream of P. chrysosporium and C. thermocellum in a sequential bioprocess to produce butanol. Butanol is an attractive biofuel because it can be utilized without modifying current transportation infrastructure. Butanol is …


The Influence Of Media Ingredients And Ph On The Growth Of Chloromonas Rosae Var. Psychrophila, Caoli Zheng 2015 University of Kentucky

The Influence Of Media Ingredients And Ph On The Growth Of Chloromonas Rosae Var. Psychrophila, Caoli Zheng

Theses and Dissertations--Biosystems and Agricultural Engineering

In order to utilize algae to fix CO2 and reduce Greenhouse gas emissions, large-scale, year-round algae cultivation will be required. This will require alga species that thrive in colder weather with media recipes based on commercially available fertilizers. The objectives of this study were to evaluate the effect of using commercial based media and the effect of pH when growing the cold-temperature algae Chloromonas rosae var. psychrophila. Commercial grade media ingredients urea, potash, triple superphosphate, and Sprint 330® were used to provide nitrogen, potassium, phosphorus, and EDTA with iron, respectively. The commercial grade media supported growth similar to the growth …


Testing The Efficiency Of A Series Hybrid Drivetrain For Agricultural Applications, Joseph W. Jackson 2015 University of Kentucky

Testing The Efficiency Of A Series Hybrid Drivetrain For Agricultural Applications, Joseph W. Jackson

Theses and Dissertations--Biosystems and Agricultural Engineering

Because of high fuel costs and rising concern over controlling motor vehicle emissions, there has been a surge in the number of hybrid passenger vehicles on roads in recent years. This transition has not yet been seen with agricultural vehicles. With this in mind, this study created a test scheme to characterize and replicate agricultural loads, and design of a hybrid drivetrain that is suitable for agricultural purposes.

Torque and power data were recorded from the controller area network of a tractor performing a baling operation. The recorded data was characterized using statistical and time series analyses, and converted into …


Development Of Techniques For Assessing And Restoring Streams On Surface Mined Lands, Whitney Cole Blackburn-Lynch 2015 University of Kentucky

Development Of Techniques For Assessing And Restoring Streams On Surface Mined Lands, Whitney Cole Blackburn-Lynch

Theses and Dissertations--Biosystems and Agricultural Engineering

Surface mining is a commonly used method for extracting coal in the Appalachian Coalfields of the U.S. This mining practice produces excess spoil or overburden, which is often placed in adjacent valleys resulting in the creation of valley fills. These valley fills bury headwater streams, which in turn can negatively impact downstream ecosystems. In 2008, the University of Kentucky designed and constructed 1,020 m of ephemeral, intermittent and headwater streams on an existing valley fill (Guy Cove) as a proof-of-concept. The goal of the project was to evaluate whether or not a stream recreation could occur on mined lands, particularly …


Optimal Uses Of Biomass Resources In Distributed Applications, Joshua J. Jackson 2015 University of Kentucky

Optimal Uses Of Biomass Resources In Distributed Applications, Joshua J. Jackson

Theses and Dissertations--Biosystems and Agricultural Engineering

Biomass production is spatially distributed resulting in high transportation costs when moving dedicated biomass crops and crop residues. A multifaceted approach was taken to address this issue as the low bulk and energy density of biomass limits transportation efficiency. Two systems were analyzed for the conversion of biomass into a denser feedstock applicable to on-farm use. Pelletization was able to densify the material into a solid fuel. Using a pilot scale flat ring pellet mill, the density of the material was able to be increased to at least 4.4 times that of uncompressed material. Pellet durability was found to be …


Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith 2015 University of Kentucky

Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith

Theses and Dissertations--Biosystems and Agricultural Engineering

Biochars have been created and characterized from a variety livestock manure biomass including poultry, dairy, and swine. However, no research has been conducted on the physical and chemical makeup of biochar pyrolyzed from horse muck. Two horse muck derived biochars containing either straw (HS) or woodchip (HW) bedding were pyrolyzed at 700°C and characterized for their physical and chemical properties. Tests revealed both biochars had high alkalinity, moderate specific conductivity, and low surface area as compared to other biochars in the literature. HS contained more mineral structures than HW. Scanning electron microscopy presented differences in particle shape, size, and presence …


Evaluation Of Sixteen Reference Evapotranspiration Methods Under Sahelian Conditions In The Senegal River Valley, Koffi Djaman, Alpha B. Balde, Abdoulaye Sow, Bertrand Muller, Suat Irmak, Mamadou K. N'Diaye, Baboucarr Manneh, Yonnelle D. Moukoumbi, Koichi Futakuchi, Kazuki Saito 2015 Africa Rice Center

Evaluation Of Sixteen Reference Evapotranspiration Methods Under Sahelian Conditions In The Senegal River Valley, Koffi Djaman, Alpha B. Balde, Abdoulaye Sow, Bertrand Muller, Suat Irmak, Mamadou K. N'Diaye, Baboucarr Manneh, Yonnelle D. Moukoumbi, Koichi Futakuchi, Kazuki Saito

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Study region: Reference evapotranspiration (ETo) plays a key role in irrigation sys-tems design, water management under irrigated and rainfed production. Underthe sahelian conditions in the Senegal River Valley that receives less than 300 mmannual rainfall, rice crop water use should be estimated for the sustainability of theresource. Study focus: However the Penman–Monteith (PM) equation is revealed the mostaccurate one; it necessitates several climatic parameters that are not always avail-able mostly in the developing countries. The objective of this study was to evaluatethe performance of 16 ETo equations against the ASCE-PM equation under thesahelian conditions at Ndiaye and Fanaye …


Agriculture And Biological Engineering/ Graduate Students/ Bracke & Fosmire/ Purdue University/ 2012, Marianne S. Bracke, Michael Fosmire 2015 Purdue University

Agriculture And Biological Engineering/ Graduate Students/ Bracke & Fosmire/ Purdue University/ 2012, Marianne S. Bracke, Michael Fosmire

Data Information Literacy Case Study Directory

This case study was comprised of three workshops for a lab in Agricultural and Biological Engineering. Data Information Literacy topics covered include developing and following a standard operating procedure, search for external data and creating metadata. Materials include a book chapter describing the case study, a data archiving checklist to follow for describing the types of data generated by the lab, a metadata entry form and an evaluation form used to gather feedback for each of the three sessions.


Development Of A Turbidity Prediction Methodology For Runoff-Erosion Models, Sagar Neupane, Jason R. Vogel, Daniel E. Storm, Bill J. Barfield, Aaron R. Mittelstet 2015 Oklahoma State University

Development Of A Turbidity Prediction Methodology For Runoff-Erosion Models, Sagar Neupane, Jason R. Vogel, Daniel E. Storm, Bill J. Barfield, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Surface water bodies can be impaired by turbidity and excessive sediment loading due to urban development, construction activities, and agricultural practices. Turbidity has been considered as a proxy for evaluating water quality, aquatic habitat, and aesthetic impairments in surface waters. The US Environment Protection Agency (USEPA) has listed turbidity and sediment as major pollutants for construction site effluent. Recently proposed USEPA regulations for construction site runoff led to increased interest in methods to predict turbidity in runoff based on parameters that are more commonly predicted in runoff- erosion models. In this study, a turbidity prediction methodology that can be easily …


Robust Estimates Of Soil Moisture And Latent Heat Flux Coupling Strength Obtained From Triple Collocation, Wade T. Crow, Fangni Lei, Christopher R. Hain, Martha C. Anderson, Russell L. Scott, David P. Billesbach, Timothy Arkebauer 2015 Hydrology and Remote Sensing Laboratory, USDA ARS, Beltsville, Maryland

Robust Estimates Of Soil Moisture And Latent Heat Flux Coupling Strength Obtained From Triple Collocation, Wade T. Crow, Fangni Lei, Christopher R. Hain, Martha C. Anderson, Russell L. Scott, David P. Billesbach, Timothy Arkebauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Land surface models (LSMs) are often applied to predict the one-way coupling strength between surface soil moisture (SM) and latent heat (LH) flux. However, the ability of LSMs to accurately represent such coupling has not been adequately established. Likewise, the estimation of SM/LH coupling strength using ground-based observational data is potentially compromised by the impact of independent SM and LH measurements errors. Here we apply a new statistical technique to acquire estimates of one-way SM/LH coupling strength which are nonbiased in the presence of random error using a triple collocation approach based on leveraging the simultaneous availability of independent SM …


Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker 2015 Oklahoma State University

Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Crop yields and salinity levels in the North Fork of the Red River (North Fork River) basin, located in southwestern Oklahoma and the Texas Panhandle, were analyzed based on the diverse climate in the region. Saline irrigation water is a major problem in the basin. The Elm Fork Creek flows through salt deposits, making the creek and its receiving stream, the North Fork River, too saline to use for irrigation. This greatly reduces the number of hectares that can be utilized for agricultural crops within the basin. A baseline SWAT model was setup, calibrated and validated to simulate streamflow and …


Identifying Intracellular Pdna Losses From A Model Of Nonviral Gene Delivery, Timothy Michael Martin, Beata Joanna Wysocki, Tadeusz Antoni Wysocki, Angela K. Pannier 2015 University of Nebraska-Lincoln

Identifying Intracellular Pdna Losses From A Model Of Nonviral Gene Delivery, Timothy Michael Martin, Beata Joanna Wysocki, Tadeusz Antoni Wysocki, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nonviral gene delivery systems are a type of nanocommunication system that transmit plasmid packets (i.e., pDNA packets) that are programmed at the nanoscale to biological systems at the microscopic cellular level. This engineered nanocommunication system suffers large pDNA losses during transmission of the genetically encoded information, preventing its use in biotechnological and medical applications. The pDNA losses largely remain uncharacterized, and the ramifications of reducing pDNA loss from newly designed gene delivery systems remain difficult to predict. Here, the pDNA losses during primary and secondary transmission chains were identified utilizing a MATLAB model employing queuing theory simulating delivery of pEGFPLuc …


Narrow Grass Hedges Reduce Tylosin And Associated Antimicrobial Resistance Genes In Agricultural Runoff, Bhavneet Soni, Shannon L. Bartelt-Hunt, Daniel D. Snow, John E. Gilley, Bryan Woodbury, David B. Marx, Xu Li 2015 University of Nebraska-Lincoln

Narrow Grass Hedges Reduce Tylosin And Associated Antimicrobial Resistance Genes In Agricultural Runoff, Bhavneet Soni, Shannon L. Bartelt-Hunt, Daniel D. Snow, John E. Gilley, Bryan Woodbury, David B. Marx, Xu Li

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Agricultural runoff from areas receiving livestock manure can potentially contaminate surface water with antimicrobials and antimicrobial resistance genes (ARGs). The objective of this study was to investigate the effectiveness of narrow grass hedges (NGHs) on reducing the transport of antimicrobials and ARGs in runoff after land application of swine manure slurry. Plot-scale rainfall simulation tests were conducted on 0.75 m by 4.0 m plots designed to test three treatment factors: manure amendment (control plots receiving no manure vs. amended plots receiving manure based on 3 times N requirement), NGH (plots with a NGH vs. plots without a NGH), and rainfall …


Vulnerability Of Crops And Native Grasses To Summer Drying In The U.S. Southern Great Plains, Naama Raz-Yaseef, Dave P. Billesbach, Marc L. Fischer, Sebastien C. Biraud, Stacey A. Gunter, James A. Bradford, Margaret S. Torn 2015 Lawrence Berkeley National Laboratory

Vulnerability Of Crops And Native Grasses To Summer Drying In The U.S. Southern Great Plains, Naama Raz-Yaseef, Dave P. Billesbach, Marc L. Fischer, Sebastien C. Biraud, Stacey A. Gunter, James A. Bradford, Margaret S. Torn

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The Southern Great Plains are characterized by a fine-scale mixture of different land-cover types, predominantly winter-wheat and grazed pasture, with relatively small areas of other crops, native prairie, and switchgrass. Recent droughts and predictions of increased drought in the Southern Great Plains, especially during the summer months, raise concern for these ecosystems. We measured ecosystem carbon and water fluxes with eddy-covariance systems over cultivated cropland for 10 years, and over lightly grazed prairie and new switchgrass fields for 2 years each. Growing-season precipitation showed the strongest control over net carbon uptake for all ecosystems, but with a variable effect: grasses …


A Sustainable Slashing Industry Using Biodegradable Sizes From Modified Soy Protein To Replace Petro-Based Poly(Vinyl Alcohol), Yi Zhao, Yuzhu Zhao, Helan Xu, Yiqi Yang 2015 University of Nebraska–Lincoln

A Sustainable Slashing Industry Using Biodegradable Sizes From Modified Soy Protein To Replace Petro-Based Poly(Vinyl Alcohol), Yi Zhao, Yuzhu Zhao, Helan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Biodegradable sizing agents from triethanolamine (TEA) modified soy protein could substitute poly(vinyl alcohol)(PVA) sizes for high-speed weaving of polyester and polyester/cotton yarns to substantially decrease environmental pollution and impel sustainability of textile industry. Nonbiodegradable PVA sizes are widely used and mainly contribute to high chemical oxygen demand (COD) in textile effluents. It has not been possible to effectively degrade, reuse or replace PVA sizes so far. Soy protein with good biodegradability showed potential as warp sizes in our previous studies. However, soy protein sizes lacked film flexibility and adhesion for required high-speed weaving. Additives with multiple hydroxyl groups, nonlinear molecule, …


Sustainable And Hydrolysis-Free Dyeing Process For Polylactic Acid Using Nonaqueous Medium, Suxin Xu, Jiangang Chen, Bijia Wang, Yiqi Yang 2015 Donghua University

Sustainable And Hydrolysis-Free Dyeing Process For Polylactic Acid Using Nonaqueous Medium, Suxin Xu, Jiangang Chen, Bijia Wang, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A sustainable and hydrolysis-free dyeing process was developed for polylactic acid (PLA) fibers. PLA is a biobased alternative to petroleum based polyethylene terephthalate (PET), which is the most widely used textile fiber. However, the hydrolytic degradation of PLA fibers under the conventional aqueous dyeing conditions limited its applications in textile industry. A new solvent dyeing process was developed using liquid paraffin as a nonaqueous dyeing medium. High quality dyed PLA fabrics were obtained without consuming water and auxiliaries. Minimal strength loss of dyed fibers was achieved by postheat setting treatment. The 3R principle (reduce, reuse, and recycle) was implemented to …


Chitosan-Alginate Scaffold Cell Culture System And Related Methods, Miqin Zhang, Forrest Kievit, Matthew Chi-hang Leung, Stephen Florczyk 2015 University of Nebraska - Lincoln

Chitosan-Alginate Scaffold Cell Culture System And Related Methods, Miqin Zhang, Forrest Kievit, Matthew Chi-Hang Leung, Stephen Florczyk

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Methods for culturing cancer cells in vitro using a three dimensional scaffold, scaffolds that include the cultured cancer cells, and methods for using the cultured cancer cells and the scaffolds that include the cultured cancer cells in anticancer therapeutic drug development.


Nanoparticle Mediated Silencing Of Dna Repair Sensitizes Pediatric Brain Tumor Cells To Y-Irradiation, Forrest M. Kievit, Zachary R. Stephen, Kui Wang, Christopher J. Dayringer, Jonathan G. Sham, Richard G. Ellenbogen, John R. Silber, Miqin Zhang 2015 University of Washington

Nanoparticle Mediated Silencing Of Dna Repair Sensitizes Pediatric Brain Tumor Cells To Y-Irradiation, Forrest M. Kievit, Zachary R. Stephen, Kui Wang, Christopher J. Dayringer, Jonathan G. Sham, Richard G. Ellenbogen, John R. Silber, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Medulloblastoma (MB) and ependymoma (EP) are the most common pediatric brain tumors, afflicting 3000 children annually. Radiotherapy (RT) is an integral component in the treatment of these tumors; however, the improvement in survival is often accompanied by radiation-induced adverse developmental and psychosocial sequelae. Therefore, there is an urgent need to develop strategies that can increase the sensitivity of brain tumors cells to RT while sparing adjacent healthy brain tissue. Apurinic endonuclease 1 (Ape1), an enzyme in the base excision repair pathway, has been implicated in radiation resistance in cancer. Pharmacological and specificity limitations inherent to small molecule inhibitors of Ape1 …


Underwater Sound Transmission Through Arrays Of Disk Cavities In A Soft Elastic Medium, David C. Calvo, Abel L. Thangawng, Christopher N. Layman Jr., Riccardo Casalini, Shadi F. Othman 2015 Acoustics Division, Naval Research Laboratory, Washington, DC

Underwater Sound Transmission Through Arrays Of Disk Cavities In A Soft Elastic Medium, David C. Calvo, Abel L. Thangawng, Christopher N. Layman Jr., Riccardo Casalini, Shadi F. Othman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Scattering from a cavity in a soft elastic medium, such as silicone rubber, resembles scattering from an underwater bubble in that low-frequency monopole resonance is obtainable in both cases. Arrays of cavities can therefore be used to reduce underwater sound transmission using thin layers and low void fractions. This article examines the role of cavity shape by microfabricating arrays of disk-shaped air cavities into single and multiple layers of polydimethylsiloxane. Comparison is made with the case of equivalent volume cylinders which approximate spheres. Measurements of ultrasonic underwater sound transmission are compared with finite element modeling predictions. The disks provide a …


Center Pivot Sprinkler Distribution Uniformity Impacts On The Spatial Variability Of Evapotranspiration, Daniel Howes, Sean Ellenson, Lucas Hoffmann, Franklin Gaudi 2014 California Polytechnic State University - San Luis Obispo

Center Pivot Sprinkler Distribution Uniformity Impacts On The Spatial Variability Of Evapotranspiration, Daniel Howes, Sean Ellenson, Lucas Hoffmann, Franklin Gaudi

BioResource and Agricultural Engineering

Understanding variable evapotranspiration (ET) throughout a field can help maximize yield on a per-acre basis, as well as assist with proper irrigation scheduling. The results from this study indicate that irrigation system distribution uniformity (DU) has a significant effect on the uniformity of ET during water-stressed periods. The study site involved intensely managed forage (alfalfa and winter grain hay) irrigated by center pivots being supplied with reclaimed water near Palmdale, California. During spring and early summer 2007 the center pivots were operating under deficit irrigation. In 2010, after the installation of reservoirs, water was applied to meet full evapotranspiration (ETc) …


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