Bioresource and Agricultural Engineering Commons^™

Toward Biochemical Conversion Of Lignocellulose On-Farm: Pretreatment And Hydrolysis Of Corn Stover In Situ, Alicia A. Modenbach, Sue E. Nokes, Michael D. Montross, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L^-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment …

Impact Of Climate Change On Extreme Hydrological Events In The Kentucky River Basin, Somsubhra Chattopadhyay

Theses and Dissertations--Biosystems and Agricultural Engineering

Anthropogenic activities including urbanization, rapid industrialization, deforestation and burning of fossil fuels are broadly agreed on as primary causes for ongoing climate change. Scientists agree that climate change over the next century will continue to impact water resources with serious implications including storm surge flooding and a sea level rise projected for North America. To date, the majority of climate change studies conducted across the globe have been for large-sized watersheds; more attention is required to assess the impact of climate change on smaller watersheds, which can help to better frame sustainable water management strategies.

In the first of three …

Fractionation And Characterization Of Lignin Streams From Genetically Engineered Switchgrass, Enshi Liu

Theses and Dissertations--Biosystems and Agricultural Engineering

Development of biomass feedstocks with desirable traits for cost-effective conversion is one of the main focus areas in biofuels research. As suggested by techno-economic analyses, the success of a lignocellulose-based biorefinery largely relies on the utilization of lignin to generate value-added products, i.e. fuels and chemicals. The fate of lignin and its structural/compositional changes during pretreatment have received increasing attention; however, the effect of genetic modification on the fractionation, depolymerization and catalytic upgrading of lignin from genetically engineered plants is not well understood. This study aims to fractionate and characterize the lignin streams from a wild-type and two genetically engineered …

Relative Cross Track Error Calculations In Asabe/Iso 12188-2:2012 And Power/Energy Analysis Using A 20 Hp Tractor On A Fully Electric Drivetrain, Joseph D. Rounsaville

Theses and Dissertations--Biosystems and Agricultural Engineering

ASABE/ISO Standard 12188-2 provides test procedures for positioning and guidance systems in agricultural vehicles during straight and level travel. The standard provides excellent descriptions of test procedures, however it does not provide detail on methods to carry out the calculations necessary to calculate relative cross-track error (XTE), which is the primary measurement used to judge accuracy of the system. The standard was used to estimate the guidance accuracy of a relatively low-accuracy vehicle at 1.25 and 0.5 m s^-1. At 1.25 m s^-1, a nearest point calculation overestimated mean XTE by 0.8 cm, or 8.2%. The …

Using The Vehicle Routing Problem (Vrp) To Provide Logistics Solutions In Agriculture, Hasan Seyyedhasani

Theses and Dissertations--Biosystems and Agricultural Engineering

Agricultural producers consider utilizing multiple machines to reduce field completion times for improving effective field capacity. Using a number of smaller machines rather than a single big machine also has benefits such as sustainability via less compaction risk, redundancy in the event of an equipment failure, and more flexibility in machinery management. However, machinery management is complicated due to logistics issues.

In this work, the allocation and ordering of field paths among a number of available machines have been transformed into a solvable Vehicle Routing Problem (VRP). A basic heuristic algorithm (a modified form of the Clarke-Wright algorithm) and a …

Performance Of Seed Treatments And In-Furrow At-Plant Insecticides For Protection Against Cry3bb1-Resistant Western Corn Rootworm, 2015, Jonas Victor De Macedo,, Kayla A. Mollet, Julie A. Peterson

Department of Entomology: Faculty Publications

The western corn rootworm is an important pest of corn that can compromise yield by feeding on plant roots during its larval stage. WCRW management has been complicated by the development of resistance in some regions, including Nebraska, to transgenic Bacillus thuringiensis (Bt) traits, particularly the protein Cry3Bb1, which confers cross-resistance to mCry3A. A field trial was established to evaluate the efficacy of neonicotinoid seed treatments in combination with in-furrow insecticides on a corn hybrid expressing mCry3A Bt proteins against corn rootworm in an area with a history of rootworm resistance. The trial was conducted in a farmer’s field in …

Pumping Plant Performance, Derrel L. Martin, William Kranz, Suat Irmak, Daran Rudnick, Chuck Burr, Steve Melvin

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Irrigation accounts for a large portion of the energy used in Nebraska agriculture. This paper describes a method to estimate the cost of pumping water and compares the amount of energy used by a properly designed and well-maintained pumping plant, represented by the Nebraska Pumping Plant Performance Criteria (NPPPC). The results can help determine the feasibility of repairing the pumping plant. Methods to compare energy sources are also presented. We recommend that you periodically arrange with a well drilling company to test the efficiency of your pump. Worksheets for pumping plant performance are included in the appendix.

Moving Integrated Weed Management From Low Level To A Truly Integrated And Highly Specific Weed Management System Using Advanced Technologies, S. L. Young, S. K. Pitla, F. K. Van Evert, J. K. Schueller, F. J. Pierce

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Integrated weed management (IWM) is one of the most commonly referred to approaches for sustainable and effective weed control in agriculture, yet it is not widely practiced, likely because current IWM systems fail to meet performance expectations of growers. The effectiveness and value of IWM systems should increase with increasing application specificity and true integration made possible with contemporary advances in technology, information systems and decision support. IWM systems can be classified based on their degree of application specificity and level of integration of tactics. In the application specificity pathway, a tactic is applied at a range of scales, from …

Optical Probing Of Gastrocnemius In Patients With Peripheral Artery Disease Characterizes Myopathic Biochemical Alterations And Correlates With Stage Of Disease, Ryan A. Becker, Kim Cluff, Nithyanandhi Duraisamy, Hootan Mehraein, Hussam Farhoud, Tracie Collins, George P. Casale, Iraklis I. Pipinos, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral artery disease (PAD) is a condition caused by atherosclerotic blockages in the arteries supplying the lower limbs and is characterized by ischemia of the leg, progressive myopathy, and increased risk of limb loss. The affected leg muscles undergo significant changes of their biochemistry and metabolism including variations in the levels of many key proteins, lipids, and nucleotides. The mechanisms behind these changes are poorly understood. The objective of this study was to correlate the severity of the PAD disease stage and associated hemodynamic limitation (determined by the ankle brachial index, ABI) in the legs of the patients with alterations …

A Comprehensive Dosimetric Study On Switching From A Type-B To A Type-C Dose Algorithm For Modern Lung Sbrt, Christina Zhou, Nathan Bennion, Rongtao Ma, Xiaoying Liang, Shuo Wang, Kristina Zvolanek, Megan Hyun, Xiaobo Li, Sumin Zhou, Weining Zhen, Chi Lin, Andrew Wahl, Dandan Zheng

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Type-C dose algorithms provide more accurate dosimetry for lung SBRT treatment planning. However, because current dosimetric protocols were developed based on conventional algorithms, its applicability for the new generation algorithms needs to be determined. Previous studies on this issue used small sample sizes and reached discordant conclusions. Our study assessed dose calculation of a Type-C algorithm with current dosimetric protocols in a large patient cohort, in order to demonstrate the dosimetric impacts and necessary treatment planning steps of switching from a Type-B to a Type-C dose algorithm for lung SBRT planning.

Methods: Fifty-two lung SBRT patients were included, each …

Creation Of An Injectable In Situ Gelling Native Extracellular Matrix For Nucleus Pulposus Tissue Engineering, Rebecca A. Wachs, Ella N. Hoogenboezem, Hammad I. Huda, Shangjing Xin, Stacy L. Porvasnik, Christine E. Schmidt

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background Context: Disc degeneration is the leading cause of low back pain and is often characterized by a loss of disc height, resulting from cleavage of chondroitin sulfate proteoglycans (CSPGs) present in the nucleus pulposus. Intact CSPGs are critical to water retention and maintenance of the nucleus osmotic pressure. Decellularization of healthy nucleus pulposus tissue has the potential to serve as an ideal matrix for tissue engineering of the disc because of the presence of native disc proteins and CSPGs. Injectable in situ gelling matrices are the most viable therapeutic option to prevent damage to the anulus fibrosus and future …

Tractor Power Take-Off Torque Measurement And Data Acquisition System, James Bw Roeber, Santosh Pitla, Roger M. Hoy, Joe D. Luck, Michael F. Kocher

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With the mechanization of agricultural operations, agricultural machinery management has become an extensive research field. Sizing tractors and implements to provide the most efficient power transfer has become an ongoing process with advances in technology. Utilization of the rotational power transferred through gear trains from the tractor engine to the power take-off (PTO) shaft is one of the most efficient methods of power transfer to an implement. This research used commercially available torque sensors that were installed on a tractor PTO shaft for measuring the torque delivered to an implement. The torque sensor was calibrated using the Nebraska Tractor Test …

Chitosan-Zein Nano-In-Microparticles Capable Of Mediating In Vivo Transgene Expression Following Oral Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The oral route is an attractive delivery route for the administration of DNA-based therapeutics, specifically for applications in gene therapy and DNA vaccination. However, oral DNA delivery is complicated by the harsh and variable conditions encountered throughout gastrointestinal (GI) transit, leading to degradation of the delivery vector and DNA cargo, and subsequent inefficient delivery to target cells. In this work, we demonstrate the development and optimization of a hybrid-dual particulate delivery system consisting of two natural biomaterials, zein (ZN) and chitosan (CS), to mediate oral DNA delivery. Chitosan-Zein Nano-in-Microparticles (CS-ZN-NIMs), consisting of core Chitosan/DNA nanoparticles (CS/DNA NPs) prepared by ionic …

A Case Study Of Field-Scale Maize Irrigation Patterns In Western Nebraska: Implications For Water Managers And Recommendations For Hyper-Resolution Land Surface Modeling, Justin P. Gibson, Trenton E. Franz, Tiejun Wang, John Gates, Patricio Grassini, Haishun Yang, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In many agricultural regions, the human use of water for irrigation is often ignored or poorly represented in land surface models (LSMs) and operational forecasts. Because irrigation increases soil moisture, feedback on the surface energy balance, rainfall recycling, and atmospheric dynamics is not represented and may lead to reduced model skill. In this work, we describe four plausible and relatively simple irrigation routines that can be coupled to the next generation of hyper-resolution LSMs operating at scales of 1 km or less. The irrigation output from the four routines (crop model, precipitation delayed, evapotranspiration replacement, and vadose zone model) is …

Crop Classification And Lai Estimation Using Original And Resolution-Reduced Images From Two Consumer-Grade Cameras, Jian Zhang, Chenghai Yang, Biquan Zhao, Huaibo Song, Wesley Clint Hoffmann, Yeyin Shi, Dongyan Zhang, Guozhong Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Consumer-grade cameras are being increasingly used for remote sensing applications in recent years. However, the performance of this type of cameras has not been systematically tested and well documented in the literature. The objective of this research was to evaluate the performance of original and resolution-reduced images taken from two consumer-grade cameras, a RGB camera and a modified near-infrared (NIR) camera, for crop identification and leaf area index (LAI) estimation. Airborne RGB and NIR images taken over a 6.5-square-km cropping area were mosaicked and aligned to create a four-band mosaic with a spatial resolution of 0.4 m. The spatial resolution …

Nanoparticle For Targeting Brain Tumors And Delivery Of O6 - Benzylguanine, University Of Washington, Seattle

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nanoparticle having a crosslinked chitosan - polyethylene oxide oligomer copolymer coating to which O⁶ - benzylgua nine is covalently coupled , compositions that include the nanoparticle , and methods for using the nanoparticle to treat brain cancers .

Evaluation Of Valiantzas’ Simplified Forms Of The Fao-56 Penman-Monteith Reference Evapotranspiration Model In A Humid Climate, Koffi Djaman, Daran Rudnick, Valere C. Mel, Denis Mutiibwa, Lamine Diop, Mamadou Sall, Isa Kabenge, Ansoumana Bodian, Hossein Tabari, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The unavailability of some meteorological variables, especially solar radiation and wind speed, is the main constraint for reference evapotranspiration (ETo) estimation using the standard United Nations Food and Agriculture Organization (FAO) Penman–Monteith (FAO-PM) equation in most developing countries. The application of ETo methods with fewer input requirements is necessary under limited climatic data conditions. The FAO-PM method under limited data conditions and nine of Valiantzas’ equations were evaluated for daily ETo estimation in a humid climate in Uganda. The FAO-PM method with missing relative humidity data performed very well across Uganda, whereas using the long-term local wind speed average values …

Crop Canopy Sensor Orientation For Late Season Nitrogen Determination In Corn, T. M. Shaver, Greg Kruger, Daran Rudnick

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Increasing nitrogen use efficiency (NUE) in irrigated corn production is of great importance to overall agricultural sustainability. Studies have shown that crop canopy sensors can aid in this pursuit as they allow for the determination of nitrogen (N) requirements in split applications later in the growing season. Fertigation can also increase NUE as many split applications can be conducted. If crop canopy sensors could be used to direct N fertigation rates, overall NUE may be increased even further. However, in some cases, N differences may need to be determined later in the growing season after corn has tasseled, which can …

Spatial And Temporal Changes In Maize And Soybean Grain Yield, Precipitation Use Efficiency, And Crop Water Productivity In The U.S. Great Plains, Meetpal S. Kukal, S. Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Sustainable agricultural utilization of the limited water resources demands improvements in understanding the changes in crop water productivity (CWP) in space and time, which is often presented as a potential solution to relieve the growing pressure on fresh water resources. In addition, crop yield needs to be studied in relation to precipitation received annually and during the growing season for its contribution to reduce irrigation water requirements, which is quantified through precipitation use efficiency (PUE). Hence, systematic quantifications, mapping, and analyses of large-scale CWP and PUE levels are needed. This study aims to quantify long-term (1982-2013) information on grain yield, …

In Vitro Porcine Blastocyst Development In Three-Dimensional Alginate Hydrogels, Jeremy R. Miles, Taylor D. Laughlin, Catherine Sargus-Patino, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Appropriate embryonic and fetal development significantly impact pregnancy success and, therefore, the efficiency of swine production. The pre-implantation period of porcine pregnancy is characterized by several developmental hallmarks, which are initiated by the dramatic morphological change that occurs as pig blastocysts elongate from spherical to filamentous blastocysts. Deficiencies in blastocyst elongation contribute to approximately 20% of embryonic loss, and have a direct influence on within-litter birth weight variation. Although factors identified within the uterine environment may play a role in blastocyst elongation, little is known about the exact mechanisms by which porcine (or other species’) blastocysts initiate and progress through …