Organisms Commons^™

Variation In Air Sac Morphology And Postcranial Skeletal Pneumatization Patterns In The African Grey Parrot, Adam B. Lawson, Aracely Martinez, Brandon P. Hedrick, M. Scott Echols, Emma R. Schachner

School of Graduate Studies Faculty Publications

The anatomy of the avian lower respiratory system includes a complex interaction between air-filled pulmonary tissues, pulmonary air sacs, and much of the postcranial skeleton. Hypotheses related to the function and phylogenetic provenance of these respiratory structures have been posed based on extensive interspecific descriptions for an array of taxa. By contrast, intraspecific descriptions of anatomical variation for these features are much more limited, particularly for skeletal pneumatization, and are essential to establish a baseline for evaluating interspecific variation. To address this issue, we collected micro-computed tomography (μCT) scans of live and deceased African grey parrots (Psittacus erithacus) to assess …

Cryo-Em Analysis Of Pseudomonas Phage Pa193 Structural Components, Stephano M. Iglesias, Chun-Feng Hou, Johnny Reid, Evan Schauer, Renae Geier, Angela Soriaga, Lucy Sim, Lucy Gao, Julian Whitelegge, Pierre Kyme, Deborah Birx, Sebastien Lemire, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

The World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various clinical settings, commonly called phage therapy, to address this growing public health crisis. Here, we describe a high-resolution structural atlas of a therapeutic, contractile-tailed Pseudomonas phage, Pa193. We used bioinformatics, proteomics, and cryogenic electron microscopy single particle analysis to identify, annotate, and build atomic models for 21 distinct structural polypeptide chains forming the icosahedral capsid, neck, contractile tail, and baseplate. We identified a putative scaffolding protein stabilizing the interior of the capsid 5-fold …

Ophidiomyces Ophidiicola And Texas Snakes: A Field To Bench Study Of Ophidiomycosis, Kenlynn L. Volz

Biology Theses

Ophidiomyces ophidiicola (Oo), the primary cause of Ophidiomycosis, is a fungal pathogen recently detected in Texas snakes. Prior to this study, there were major knowledge gaps regarding Oo growth, spread, and suitable environmental conditions. This study filled those gaps using a field to bench approach involving sampling snakes across Texas, in vitro fungal growth, and ecological niche modeling. 103 snakes were sampled across Texas in 2023 and 2024 and six cases of Oo infection were detected. Results of the growth experiment suggest that Oo prefers more stable temperatures and high humidity to a point and that there is a …

Synovial Fluid Cutibacterium Acnes Antigen Is Detected Among Shoulder Samples With High Inflammation And Early Culture Growth, Krista Toler, Pearl Paranjape, Alex Mclaren, Thomas Jefferson University

Rothman Institute Papers

Background: An emerging paradigm suggests that positive Cutibacterium acnes shoulder cultures can result from either true infection or contamination, with true infections demonstrating a host inflammatory response and early culture growth. This clinical retrospective study examines the relationship between C. acnes antigen, C. acnes culture results, and inflammation.

Methods: From January 2021 to July 2023, 1,365 periprosthetic synovial fluid samples from 347 institutions were tested for shoulder infection at a centralized clinical laboratory. A biomarker scoring system based on the 2018 International Consensus Meeting (ICM) definition was utilized to assign each sample an inflammation score. Associations between inflammation, culture results, …

Developing Coexpression Systems To Introduce Hydroxyproline Into Protein Engineered Collagen Peptides Utilizing Hydroxylase From Acanthamoeba Polyphaga Mimivirus, Jennifer Soldatich

Theses and Dissertations

A major challenge of developing collagen mimetic peptides (CMPs) by bacterial expression is to include hydroxyproline for biomedical applications. Coexpression of Prolyl-4-hydroxylase from A.mimivirus with CMPs was investigated. Additionally, four expression designs were created for potential applications in mammalian cells to utilize their natural ability of proline-hydroxylation by post-translational modification.

Bean Yellow Mosaic Virus And Its Management In Lupins, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Bean yellow mosaic virus (BYMV) is an aphid-transmitted virus that commonly causes necrotic symptoms that kills lupin plants when infected early before pod set. When plants are infected after pod set, black pods develop (known as black pod syndrome).

BYMV is found predominantly in high rainfall areas of the Western Australian (WA) grainbelt. This page outlines the symptoms, diagnosis, factors favouring disease risk and spread, yield and quality impacts, and management for BYMV in lupin crops in WA

Blackleg And Its Management In Canola, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Blackleg, caused by the fungus Leptosphaeria maculans, is the most common and serious disease of canola in Western Australia (WA). Blackleg can cause significant damage by infecting the cotyledons or first leaves early in the season, leading ultimately to crown lesions or cankers later in the season.

Changes in variety choice, crop rotation, earlier sowing times, and stubble retention have extended the risk of blackleg disease. Because of early emergence, later stages of crop growth are more likely to be exposed and infection can occur on all parts of the plant above ground, including canola flowers, stems, and branches (this …

Net Form Net Blotch And Its Management In Barley, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Net form net blotch is a fungal disease that can reduce yield and grain quality of barley crops. It can vary in appearance depending on the pathogen, variety of barley, and stage of infection. This factsheet describes the symptoms, and how this disease is managed.

Yellow Spot And Septoria Nodorum Blotch And Their Management In Wheat, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Leaf spot diseases affecting wheat in Western Australia (WA) are yellow spot (Pyrenophora tritici-repentis), septoria nodorum blotch (Parastagonospora nodorum), and septoria tritici blotch (Zymoseptoria tritici). They are caused by 3 different fungal pathogens but the disease symptoms and biologies are similar.

Impact from leaf spot diseases vary greatly from season to season and between locations. They are a problem in continuous wheat crops in stubble retention (no-till) farming systems. For the most effective control, an integrated disease management (IDM) approach is required.

This page outlines the symptoms, diseases spread and risk factors, yield and quality losses, and management options for …

Sclerotinia Stem Rot And Its Management In Canola, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Sclerotinia stem rot, caused by the fungus Sclerotinia sclerotiorum, is a disease of canola that can cause significant yield losses exceeding 20% under conducive conditions. This disease occurs in all areas of the WA grainbelt.

Sclerotinia stem rot is one of the most variable and unpredictable diseases of canola, with incidence of infection varying greatly between paddocks and between years. Yield losses can be severe in years of higher moisture, cool conditions, and high humidity, which favour disease development.

This factsheet details the symptoms, disease risk and spread, yield and quality losses, and integrated disease management tactics for sclerotinia stem …

Barley And Cereal Yellow Dwarf Viruses And Their Management, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Barley yellow dwarf viruses (BYDV) and cereal yellow dwarf viruses (CYDV) damage cereal crops worldwide. These yellow dwarf viruses infect wheat, barley, oats and grasses and are transmitted by aphids.

This page describes these collective yellow dwarf viruses (barley and cereal yellow dwarf viruses), which damage cereals crops and advises on their diagnosis, monitoring, and management the viruses in the Western Australian (WA) grainbelt.

Growers are encouraged to check cereal crops for aphids on a regular basis, especially early in the season during autumn when winged aphids migrate into cereal crops during the crops’ most vulnerable growth phase. Early detection …

Septoria Avenae Blotch And Its Management In Oats, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Septoria avenae blotch (Phaeosphaeria avenaria f.sp. avenaria) is a stubble borne fungal disease of oats and is the most common oat disease in Western Australia and occurs throughout the cereal growing areas.It is host specific, meaning it only affects oats.

This page summarises the symptoms, factors favouring disease risk and spread, yield and quality losses, and management strategies for the disease

Leaf Diseases And Their Management In Oats, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Leaf diseases of oats impact on grain yield and quality and reduce hay quality characteristics, such as colour and digestibility. This page outlines the symptoms, factors favouring disease risk and spread, and management.

Major leaf diseases of oats are septoria avenae blotch, stem rust, leaf rust, and barley yellow dwarf virus. Their severity changes with seasons.

Foliar Diseases And Their Management In Lupins, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

This factsheet describes symptoms and management of the major fungal and viral diseases affecting lupin foliage, stems, and pods. Several of these diseases have the capacity to cause significant losses if not managed effectively.

Inoculum can be carried in soil, seed, stubble, on green regrowth, or by insect vectors, depending on the disease. Therefore, an integrated approach to disease management is required using tactics such as crop rotation, stubble management, fungicide seed dressing, variety selection, and seed testing

Managing Aphids And Viruses In Lupins, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Aphids reduce yields by direct feeding damage, which causes flower and pod abortion and occasionally plant death in lupins. The extent of damage varies between seasons, but losses can be severe in years that favour aphid population development.

Lupins are most vulnerable to aphids during budding and flowering, as severe feeding damage on growing tips can cause buds to drop, flowers to abort, and reduced pod set. Some lupin varieties are more susceptible to aphids than others.

Aphids transmit serious virus diseases, which reduce yields and contaminate seed stocks. Some species of aphids are more difficult to control than others.

Stem Rust And Its Management In Wheat, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Stem rust (Puccinia graminis f. sp. tritici) is a foliar disease that can significantly reduce wheat yields if it occurs in early spring and is not controlled. Risk factors, management strategies (including variety choices and foliar fungicides), and yield loss from different varieties are discussed here.

In Western Australia, stem rust typically causes losses of 10 to 50% in wheat, but this can increase to 90% when it occurs in early spring and is not controlled.

Stem rust affects wheat and triticale, but barley can also be a host during summer months.Stem rust produces large, reddish-brown spore masses on both …

Beneficial Effects Of Lactobacillus Plantarum On Growth Performance, Immune Status, Antioxidant Function And Intestinal Microbiota In Broilers, Xiao Xiao, Tiantian Cui, Songke Qin, Tao Wang, Jinsong Liu, Lihan Sa, Yanping Wu, Yifan Zhong, Caimei Yang

Faculty, Staff and Student Publications

Lactobacillus plantarum (L. plantarum) has been globally regarded as antibiotic alternative in animal farming in the past few years. However, the potential function of L. plantarum in broilers has not been systemically explored. In this study, a total of 560 one-day-old yellow-feathered broilers were randomly divided into 3 groups, fed with basal diet and drank with L. plantarum HJZW08 (LP) at the concentration of 0 (CON), 1000 × 10^5 (LP1000), and 2000 × 10^5 CFU/L (LP2000) for 70 d. Results showed that the body weight (BW), average daily …

Effects Of Limosilactobacillus Reuteri Strains Pta-126787 And Pta-126788 On Intestinal Barrier Integrity And Immune Homeostasis In An Alcohol-Induced Leaky Gut Model, Dharanesh Gangaiah, Min Gu, Aline Zaparte, Olaf Will, Laurie C. Dolan, Anthony Goering, Jason Pillai, Shrinivasrao P. Mane, German Plata, Emily B. Helmes, David A. Welsh, Arvind Kumar Mahajan

School of Graduate Studies Faculty Publications

Intestinal barrier is a first line of defense that prevents entry of various harmful substances from the lumen into the systemic environment. Impaired barrier function with consequent translocation of harmful substances into systemic circulation (“leaky gut”) is a central theme in many gastrointestinal, autoimmune, mental, and metabolic diseases. Probiotics have emerged as a promising strategy to maintain intestinal integrity and address “leaky gut”. Using in silico, in vitro and avian in vivo analyses, we previously showed that two novel L. reuteri strains, PTA-126787 (L. reuteri 3630) and PTA-126788 (L. reuteri 3632), isolated from broiler chickens possess favorable safety profiles. Consistent …

In Vitro Antibacterial, Antibiofilm Activities, And Phytochemical Properties Of Posidonia Oceanica (L) Delile: An Endemic Mediterranean Seagrass, Ertugrul Ozbil, Mehmet Ilktac, Sultan Ogmen, Ovgu Isbilen, Jesus M Duran Ramirez, Jana Gomez, Jennifer N Walker, Ender Volkan

Faculty, Staff and Student Publications

In the antibiotic resistance era, utilizing understudied sources for novel antimicrobials or antivirulence agents can provide new advances against antimicrobial resistant pathogens. In this study, we aimed to investigate antibacterial and antibiofilm activities of Posidonia oceanica (L.) Delile against Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 700603 and several S. aureus clinical isolates obtained from medical devices, including patient urinary catheters and breast implant infections, with varying antibiotic recalcitrance profiles. The ethanolic and methanolic extracts from P. oceanica rhizome exhibited significant antibacterial activity against E. faecalis and S. aureus, as well …

Molecular Detection Of Pathogenic Bacteria In American Dog Ticks (Dermacentor Variabilis) In Brown County, Nebraska, Nicole Messbarger, Darby Carlson, Keith Geluso, Julie Shaffer

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Ticks carry and transmit disease pathogens that cause many human illnesses. Many of these diseases in humans are accompanied by a wide variety of symptoms making tick-borne illnesses often difficult to diagnose. The rate of reported tick-borne illnesses in humans has increased during the past decades in the United States, alongside a rise in concern for public health and safety. In northern Nebraska, the American dog tick (Dermacentor variabilis) is the primary tick encountered by humans and domestic animals in the region. This species is a known vector for spotted fever group (SFG) rickettsioses and tularemia (Francisella …