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Covid-19: A One Health Issue, Karen Gruszynski 2021 Lincoln Memorial University

Covid-19: A One Health Issue, Karen Gruszynski

Cumberland Mountain Naturalist

COVID-19 has very rapidly become part of modern societal lexicon as the disease highlights the importance understanding zoonotic diseases and the impact they can have on human life. With a 177million human cases and 3.82million deaths globally at the time of this publication, the "One Health" nature of the disease has had a lasting impact across the planet. As the disease spread rapidly, it highlighted the dissemination of information through social media and other sources. With this in mind, Dr. Gary Vroegindewey, Director for One Health at LMU-CVM, put forth the idea to have students collect and summarize information ...


Successful Atac-Seq From Snap-Frozen Equine Tissues, Sichong Peng, Rebecca Bellone, Jessica L. Petersen, Ted S. Kalbfleisch, Carrie J. Finno 2021 University of California, Davis

Successful Atac-Seq From Snap-Frozen Equine Tissues, Sichong Peng, Rebecca Bellone, Jessica L. Petersen, Ted S. Kalbfleisch, Carrie J. Finno

Veterinary Science Faculty Publications

An assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) has become an increasingly popular method to assess genome-wide chromatin accessibility in isolated nuclei from fresh tissues. However, many biobanks contain only snap-frozen tissue samples. While ATAC-seq has been applied to frozen brain tissues in human, its applicability in a wide variety of tissues in horse remains unclear. The Functional Annotation of Animal Genome (FAANG) project is an international collaboration aimed to provide high quality functional annotation of animal genomes. The equine FAANG initiative has generated a biobank of over 80 tissues from two reference female animals and experiments to begin ...


Variable Accuracy, Precision, And Consistency Of Compounded Famciclovir Formulated For Management Of Feline Herpesvirus-1 In Cats, Louise M. O'Leary, Rachel A. Allbaugh, Dwayne Schrunk, Thomas E. Olson, Lionel Sebbag 2021 Iowa State University

Variable Accuracy, Precision, And Consistency Of Compounded Famciclovir Formulated For Management Of Feline Herpesvirus-1 In Cats, Louise M. O'Leary, Rachel A. Allbaugh, Dwayne Schrunk, Thomas E. Olson, Lionel Sebbag

Veterinary Clinical Sciences Publications

Objective: To evaluate compounded famciclovir suspensions for accuracy, precision, and consistency in drug content.

Procedures: Two compounded famciclovir concentrations were evaluated (250 and 400 mg/mL, 30 preparations total from nine 503A compounding pharmacies) with U.S. Food and Drug Administration (FDA)-approved famciclovir tablets as control. Drug quantification via high-performance liquid chromatography (with famciclovir reference standard and pramipexole internal standard) was performed at 0, 14, and 28 days with concentrations of 90%–110% of labeled dose considered acceptable (US Pharmacopoeia standards).

Results: FDA-approved tablets from three different manufacturers were found to be accurate and precise with acceptable drug content ...


Swine Disease Reporting System Report #40, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob McGaughey, Eric Herman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings 2021 Iowa State University

Swine Disease Reporting System Report #40, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...


Comparison Of Naturally Occurring Vs. Experimental Infection Of Staphylococcus Aureus Septicemia In Laying Hens In Two Different Age Groups, Meaghan Meyer, Elizabeth Bobeck, Yuko Sato, Mohamed El-Gazzar 2021 Iowa State University

Comparison Of Naturally Occurring Vs. Experimental Infection Of Staphylococcus Aureus Septicemia In Laying Hens In Two Different Age Groups, Meaghan Meyer, Elizabeth Bobeck, Yuko Sato, Mohamed El-Gazzar

Animal Science Publications

In April and November of 2018, multiple commercial laying hen flocks within the same company presented with a sharp increase in mortality and drop in egg production that persisted for several days. These flocks showed striking necropsy lesions consistent with systemic infection and responded to antimicrobial treatment in the feed. Staphylococcus aureus (SA) was the most frequently isolated organism from multiple tissues including comb and wattle lesions, lungs, liver, ovary, spleen, and bone marrow. Given such an uncommon presentation of SA, which is known as a secondary opportunistic pathogen, a challenge study was conducted to evaluate its role in these ...


Focused Ultrasound Of The Caudal Vena Cava In Dogs With Cavitary Effusions Or Congestive Heart Failure: A Prospective, Observational Study, Yen-Yu Chou, Jessica L. Ward, Lara Z. Barron, Shane D. Murphy, Melissa A. Tropf, Gregory R. Lisciandro, Lingnan Yuan, Jonathan P. Mochel, Teresa C. DeFrancesco 2021 Iowa State University

Focused Ultrasound Of The Caudal Vena Cava In Dogs With Cavitary Effusions Or Congestive Heart Failure: A Prospective, Observational Study, Yen-Yu Chou, Jessica L. Ward, Lara Z. Barron, Shane D. Murphy, Melissa A. Tropf, Gregory R. Lisciandro, Lingnan Yuan, Jonathan P. Mochel, Teresa C. Defrancesco

Biomedical Sciences Publications

Introduction: Ultrasonographic indices of the inferior vena cava are useful for predicting right heart filling pressures in people.

Objectives: To determine whether ultrasonographic indices of caudal vena cava (CVC) differ between dogs with right-sided CHF (R-CHF), left-sided CHF (L-CHF), and noncardiac causes of cavitary effusion (NC).

Materials and methods: 113 dogs diagnosed with R-CHF (n = 51), L-CHF (30), or NC effusion (32) were enrolled. Seventeen of the R-CHF dogs had pericardial effusion and tamponade. Focused ultrasound was performed prospectively to obtain 2-dimensional and M-mode subxiphoid measures of CVC maximal and minimal size (CVCmax and CVCmin), CVCmax indexed to aortic dimension ...


Complete Coding Genome Sequence Of A Novel Porcine Reproductive And Respiratory Syndrome Virus 2 Restriction Fragment Length Polymorphism 1-4-4 Lineage 1c Variant Identified In Iowa, Usa, Giovani Trevisan, Ganwu Li, Cesar A.A. Moura, Katie Coleman, Pete Thomas, Jianqiang Zhang, Phillip Gauger, Michael Zeller, Daniel C.L. Linhares 2021 Iowa State University

Complete Coding Genome Sequence Of A Novel Porcine Reproductive And Respiratory Syndrome Virus 2 Restriction Fragment Length Polymorphism 1-4-4 Lineage 1c Variant Identified In Iowa, Usa, Giovani Trevisan, Ganwu Li, Cesar A.A. Moura, Katie Coleman, Pete Thomas, Jianqiang Zhang, Phillip Gauger, Michael Zeller, Daniel C.L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

A porcine reproductive and respiratory syndrome virus 2 strain was identified in lung samples from nursery piglets associated with a 17.15% mortality rate on a swine farm in Iowa. Open reading frame 5 (ORF5) sequencing indicated that this strain is a restriction fragment length polymorphism (RFLP) 1-4-4 lineage 1C variant strain, and its complete coding genome sequence was determined.


Yeast Particle Encapsulation Of Scaffolded Terpene Compounds For Controlled Terpene Release, Ernesto R. Soto, Florentina Rus, Hanchen Li, Carli Garceau, Jeffrey Chicca, Mostafa Elfawal, David Gazzola, Martin K. Nielsen, Joseph F. Urban Jr., Raffi V. Aroian, Gary R. Ostroff 2021 University of Massachusetts

Yeast Particle Encapsulation Of Scaffolded Terpene Compounds For Controlled Terpene Release, Ernesto R. Soto, Florentina Rus, Hanchen Li, Carli Garceau, Jeffrey Chicca, Mostafa Elfawal, David Gazzola, Martin K. Nielsen, Joseph F. Urban Jr., Raffi V. Aroian, Gary R. Ostroff

Maxwell H. Gluck Equine Research Center Faculty Publications

Terpenes are naturally occurring compounds produced by plants that are of great commercial interest in the food, agricultural, cosmetic, and pharmaceutical industries due to their broad spectra of antibacterial, antifungal, anthelmintic, membrane permeation enhancement, and antioxidant biological activities. Applications of terpenes are often limited by their volatility and the need for surfactants or alcohols to produce stable, soluble (non-precipitated) products. Yeast particles (YPs) are hollow, porous microspheres that have been used for the encapsulation of terpenes (YP terpenes) by passive diffusion of terpenes through the porous YP cell walls. We here report the development of a second generation YP encapsulated ...


Gene Editing And Gene Therapy: Entering Uncharted Territory In Veterinary Oncology, Wesley A. Wierson, Alex M. Abel, Elizabeth L. Siegler, Stephen C. Ekker, Chad M. Johannes, Saad S. Kenderian, Jonathan P. Mochel 2021 LifEngine Animal Health Laboratories

Gene Editing And Gene Therapy: Entering Uncharted Territory In Veterinary Oncology, Wesley A. Wierson, Alex M. Abel, Elizabeth L. Siegler, Stephen C. Ekker, Chad M. Johannes, Saad S. Kenderian, Jonathan P. Mochel

Biomedical Sciences Publications

With rapid advances in gene editing and gene therapy technologies, the development of genetic, cell, or protein-based cures to disease are no longer the realm of science fiction but that of today’s practice. The impact of these technologies are rapidly bringing them to the veterinary market as both enhanced therapeutics and towards modeling their outcomes for translational application. Simply put, gene editing enables scientists to modify an organism’s DNA a priori through the use of site-specific DNA targeting tools like clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9). Gene therapy is a broader definition ...


Comparison Of Mycoplasma Hyopneumoniae Response To Infection By Route Of Exposure, Ana Paula S. Poeta Silva, Thaire P. Marostica, Aric McDaniel, Bailey L. Arruda, Carmen Alonso, Rachel J. Derscheid, Paul Yeske, Daniel C.L. Linhares, Luis Giménez-Lirola, Locke A. Karriker, Eduardo Fano, Jeffrey J. Zimmerman, Maria J. Clavijo 2021 Iowa State University

Comparison Of Mycoplasma Hyopneumoniae Response To Infection By Route Of Exposure, Ana Paula S. Poeta Silva, Thaire P. Marostica, Aric Mcdaniel, Bailey L. Arruda, Carmen Alonso, Rachel J. Derscheid, Paul Yeske, Daniel C.L. Linhares, Luis Giménez-Lirola, Locke A. Karriker, Eduardo Fano, Jeffrey J. Zimmerman, Maria J. Clavijo

Veterinary Diagnostic and Production Animal Medicine Publications

Mycoplasma hyopneumoniae (MHP) is a concern both for pig well-being and producer economic viability. In the absence of fully protective health interventions, producers rely on controlled exposure to induce an immune response in pigs and minimize the clinical outcomes of MHP infection in pig populations. This study compared the effect of route of exposure on MHP infection, antibody response, clinical signs, and pathology. Six-week-old MHP-negative pigs (n = 78) were allocated to negative control (n = 6) or one of three MHP exposure routes: intratracheal (n = 24, feeding catheter), intranasal (n = 24, atomization device), and aerosol (n = 24, fogger). Body weight ...


Nutritional Effects On The Gut Microbiome & The Brain-Gut Axis: Unlocking The Therapeutic And Preventative Potential Of Nutrition For Gut Dysbiosis Associated Diseases, Sarah Freund, Karin Allenspatch, Jonathan P. Mochel 2021 Iowa State University

Nutritional Effects On The Gut Microbiome & The Brain-Gut Axis: Unlocking The Therapeutic And Preventative Potential Of Nutrition For Gut Dysbiosis Associated Diseases, Sarah Freund, Karin Allenspatch, Jonathan P. Mochel

Biomedical Sciences Publications

Diet plays a pivotal role in the overall health of an individual. Not only does it help carry out and regulate certain physiological functions, but it also can determine the composition of the gut microbiome. While the relative number of microorganisms that make up the gut microbiome vary between individuals and can be dependent on different environmental factors, there is evidence to suggest that composition of the microbiome can correlate with overall health or disease. When the GI microbiome is disturbed or suddenly changes it results in microbiome dysbiosis, a condition that correlates with the presence of certain diseases. Diseases ...


Endocarditis Infecciosa En Canino Con Diagnóstico Previo De Síndrome Vestibular Periférico Secundario A Probable Otitis Media-Interna: Reporte De Caso, María Andrea Guzmán Solano, Pedro Vargas-Pinto, César Augusto Cabrejo Saavedra 2021 Universidad Nacional Autónoma de México

Endocarditis Infecciosa En Canino Con Diagnóstico Previo De Síndrome Vestibular Periférico Secundario A Probable Otitis Media-Interna: Reporte De Caso, María Andrea Guzmán Solano, Pedro Vargas-Pinto, César Augusto Cabrejo Saavedra

Revista de Medicina Veterinaria

La endocarditis infecciosa es un proceso patológico de baja incidencia en la clínica diaria; su principal etiología son los agentes bacterianos, los cuales colonizan con mayor prevalencia de válvula mitral y aortica. En este artículo se presenta el estudio de un perro con diagnóstico previo de síndrome vestibular periférico secundario a probable otitis media-interna. Se presenta con un soplo de aparición súbita, claudicaciones intermitentes, inapetencia, depresión y fiebre, por lo cual se hace la valoración ecocardiográfica, donde se evidencia engrosamiento valvular mitral, acompañado de estructuras hiperecoicas en el borde libre que corresponden a lesiones vegetativas. Por medio de los criterios ...


Syrup Hedera Helix Extract Poisoning In Dog: A Clinical Report, Erdem Gülersoy, Murat Kaan Durgut, Süleyman Serhat İyigün, Mutlu Sevinç 2021 Selcuk University

Syrup Hedera Helix Extract Poisoning In Dog: A Clinical Report, Erdem Gülersoy, Murat Kaan Durgut, Süleyman Serhat İyigün, Mutlu Sevinç

Revista de Medicina Veterinaria

En este informe clínico se describe un cuadro de gastroenteritis desarrollada por un golden retriever de 1 año que pesaba 24 kg, como resultado del uso aleatorio y sin receta de un extracto de jarabe de Hedera helix, que es solo para uso por humano. El diagnóstico se realizó después de que se descartaron otros factores que podrían causar gastroenteritis. Se observó una mejora en los hallazgos clínicos como resultado del tratamiento de apoyo. Ya se ha reconocido ampliamente que las saponinas triterpénicas, que son compuestos biológicos activos de Hedera helix, causan gastroenteritis en perros, y se considera que el ...


Designing And Testing Of A System For Aerosolization And Recovery Of Viable Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): Theoretical And Engineering Considerations, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks 2021 Iowa State University

Designing And Testing Of A System For Aerosolization And Recovery Of Viable Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): Theoretical And Engineering Considerations, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks

Agricultural and Biosystems Engineering Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, and ...


Swine Disease Reporting System Report #39, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob McGaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings 2021 Iowa State University

Swine Disease Reporting System Report #39, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...


Radiation Exposure Associated With Percutaneous Fluoroscopically Guided Lag Screw Fixation For Sacroiliac Luxation In Dogs, Jaron H. Naiman, Eric M. Zellner, Brian L. Petrovsky, Thomas O. Riegel, Elizabeth M. Schmitt, Lingnan Yuan, Jonathan P. Mochel, Karl H. Kraus 2021 Iowa State University

Radiation Exposure Associated With Percutaneous Fluoroscopically Guided Lag Screw Fixation For Sacroiliac Luxation In Dogs, Jaron H. Naiman, Eric M. Zellner, Brian L. Petrovsky, Thomas O. Riegel, Elizabeth M. Schmitt, Lingnan Yuan, Jonathan P. Mochel, Karl H. Kraus

Biomedical Sciences Publications

Objective: To determine radiation exposure to surgical personnel and to evaluate the accuracy of a modified percutaneous lag screw fixation technique for sacroiliac luxation (SIL) under fluoroscopic guidance in dogs.

Study design: Cadaveric experimental study.

Sample population: Seventeen beagle cadavers with iatrogenic SIL.

Methods: Seventeen beagles with iatrogenic SIL underwent reduction and stabilization with 3.5‐mm screws. Hypodermic needles (14 gauge) and fluoroscopy were used to orient two Kirschner wires for temporary stabilization and to guide drilling of glide and pilot holes using cannulated drill bits. Duration of surgery and radiation exposure were recorded. Postoperative computed tomographic evaluation of ...


Short‐Term Outcomes Of 59 Dogs Treated For Ilial Body Fractures With Locking Or Non‐Locking Plates, Brian L. Petrovsky, Taylor Knuth, Cristina Aponte‐Colón, William Hoefle, Karl H. Kraus, Jaron H. Naiman, Lingnan Yuan, Jonathan P. Mochel, Eric Zellner 2021 Iowa State University

Short‐Term Outcomes Of 59 Dogs Treated For Ilial Body Fractures With Locking Or Non‐Locking Plates, Brian L. Petrovsky, Taylor Knuth, Cristina Aponte‐Colón, William Hoefle, Karl H. Kraus, Jaron H. Naiman, Lingnan Yuan, Jonathan P. Mochel, Eric Zellner

Biomedical Sciences Publications

Objective: To determine the influence of plating systems on the clinical outcomes in dogs treated for ilial fractures.

Design: Retrospective study.

Animals: Fifty‐nine dogs (63 hemipelves).

Methods: Radiographs and medical records of dogs with ilial fractures presented to Iowa State University between 2003 and 2019 were reviewed. After fracture reduction, fractures were fixed with a locking plate system (LPS) or non‐locking plate system (NLS). Perioperative, long‐term complications, and follow‐up data were recorded. The frequency of implant failure and pelvic collapse were compared using a logistic and linear regression analysis, respectively. Where the univariate test was statistically ...


Determination Of Ownership Practices And Cytauxzoon Felis Prevalence In Domestic Felines Of Northwest Arkansas, Daniella M. Duran 2021 University of Arkansas, Fayetteville

Determination Of Ownership Practices And Cytauxzoon Felis Prevalence In Domestic Felines Of Northwest Arkansas, Daniella M. Duran

Animal Science Undergraduate Honors Theses

Cytauxzoon felis is a protozoan pathogen that causes the infectious disease, cytauxzoonosis in wild and domestic felines. The natural host of this pathogen is the bobcat (Lynx rufus), which is why this disease is also commonly referred to as “bobcat fever”. The C. felis pathogen infects wild and domestic cats upon transmission by infected ticks. Amblyomma americanum, also known as the Lone Star tick, the primary transmission vector for C. felis. This tick species is largely populated throughout the state of Arkansas, making C. felis a large threat to wild and domestic felines in these areas. Cytauxzoonosis is commonly thought ...


Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty 2021 University of Arkansas, Fayetteville

Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty

Animal Science Undergraduate Honors Theses

This study will focus on how maternal phosphorus status of beef heifers affects the growth and performance of their calves. Heifers have been offered free-choice mineral with either 0 or 4% supplemental phosphorus from 30 days after weaning until calving. A study by H. Hilfiker, a University of Arkansas honors student, investigated the effects of these treatments from 30 days after weaning until 60 days after the breeding season when heifers were confirmed to be bred or open. For this developing heifer project 64 crossbred Angus heifers were assigned randomly into 8 groups (8 heifers/ group) before assigning each group ...


Production Of Monoclonal And Polyclonal Antibodies Against Prrsv Mlvd23-S Tag Peptide Sequence, Katelyn Bettin 2021 South Dakota State University

Production Of Monoclonal And Polyclonal Antibodies Against Prrsv Mlvd23-S Tag Peptide Sequence, Katelyn Bettin

Schultz-Werth Award Papers

Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus which belongs to the family Arteriviridae. Since its emergence in the late 1980’s and early 1990’s, PRRSV has had a major economic effect on the swine industry. The most notable effects of this disease are respiratory distress in piglets and late-term pregnancy failures in sows. To track and control spread of this disease, the industry must be able determine which animals have been naturally infected with PRRSV as this contributes to major disease outbreaks and localized pandemics. While vaccines help control the spread of a disease, they ...


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