Validation Of A Lysis Buffer Containing 4 M Guanidinium Thiocyanate (Gitc)/ Triton X-100 For Extraction Of Sars-Cov-2 Rna For Covid-19 Testing: Comparison Of Formulated Lysis Buffers Containing 4 To 6 M Gitc, Roche External Lysis Buffer And Qiagen Rtl Lysis Buffer,
2020
School of Microbiology, University College Cork, College Road, Cork, Ireland
Validation Of A Lysis Buffer Containing 4 M Guanidinium Thiocyanate (Gitc)/ Triton X-100 For Extraction Of Sars-Cov-2 Rna For Covid-19 Testing: Comparison Of Formulated Lysis Buffers Containing 4 To 6 M Gitc, Roche External Lysis Buffer And Qiagen Rtl Lysis Buffer, Martina F. Scallan, Catherine Dempsey, John Mcsharry, Isabelle O'Callaghan, Paula M. O'Connor, Conor P. Horgan, Edel Durack, Paul D. Cotter, Sarah Hudson, Humphrey A. Moynihan, Brigid Lucey
Dept. of Biological Sciences Preprints
The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase in demand has resulted in a shortage of numerous reagents in particular those associated with the lysis buffer required to extract the viral RNA. Herein, we describe a rapid collective effort by hospital laboratory scientists, academic researchers and the biopharma industry to generate a validated lysis buffer. We have formulated a 4M Guanidinium thiocyanate (GITC)/ Triton X-100 Lysis buffer which provides comparable results with the recommended reagents. This buffer will ease the burden on hospital labs in their heroic efforts …
Information Mining For Covid-19 Research From A Large Volume Of Scientific Literature,
2020
Asurion
Information Mining For Covid-19 Research From A Large Volume Of Scientific Literature, Sabber Ahamed, Manar D. Samad
Computer Science Faculty Research
The year 2020 has seen an unprecedented COVID-19 pandemic due to the outbreak of a novel strain of coronavirus in 180 countries. In a desperate effort to discover new drugs and vaccines for COVID-19, many scientists are working around the clock. Their valuable time and effort may benefit from computer-based mining of a large volume of health science literature that is a treasure trove of information. In this paper, we have developed a graph-based model using abstracts of 10,683 scientific articles to find key information on three topics: transmission, drug types, and genome research related to coronavirus. A subgraph is …
Ua94/6/1 Reflection,
2020
Western Kentucky University
Ua94/6/1 Reflection, Janice Kampsen
Student/Alumni Personal Papers
Report regarding COVID-19 by Janice Kampsen for Military Science 490 class.
My Story,
2020
Taylor University
My Story, Tesia Juraschek
Reflections, Poetry, Photos, and other Writings
My first reactions to COVID as we learned that it would affect our schooling.
Ua94/6/1 Covid-19 Impacts,
2020
Western Kentucky University
Ua94/6/1 Covid-19 Impacts, Shelby Howell
Student/Alumni Personal Papers
Report on the impact of COVID-19 by student Shelby Howell for Military Science 490 class.
Holding My Hand,
2020
Taylor University
Holding My Hand, Marylou Habecker
Reflections, Poetry, Photos, and other Writings
This is a piece that I have written to try to give HOPE to others in the middle of this crisis. A story I witnessed taken from another part of the world during a horrendous time of war gave me HOPE. He is Holding My Hand.
Senior Year Cut Short,
2020
Taylor University
Senior Year Cut Short, Caleb Amick
Reflections, Poetry, Photos, and other Writings
This is my story of what happened to me during the last week of school before we left campus.
Day 10,
2020
Taylor University
Day 10, Elise Wixtrom
Reflections, Poetry, Photos, and other Writings
This is a poem I wrote after visiting the supermarket right after classes closed on Taylor's campus.
Nothing Wasted,
2020
Taylor University
Nothing Wasted, Adina Shabe
Reflections, Poetry, Photos, and other Writings
As our world is facing unprecedented interruptions to our sense of "normal" I have been pressing in to receive God's perspective for myself during this time. He is a God who wastes nothing, He uses everything.
Ua94/6/1 My Story: Wku Mens Basketball's Taveion Hollingsworth,
2020
Western Kentucky University
Ua94/6/1 My Story: Wku Mens Basketball's Taveion Hollingsworth, Taveion Hollingsworth
Student/Alumni Personal Papers
My Story is a series of first-person feature articles written by WKU student-athletes. The student-athletes will be telling their own stories in their own words. This article written by men’s basketball junior guard Taveion Hollingsworth, whose mission of advancing to the NCAA Tournament with his teammates was halted when the Covid-19 pandemic hit.
Don't Bug Me: Everything Young Kids Need To Know About Coronavirus--In Verse!,
2020
Sacred Heart University
Don't Bug Me: Everything Young Kids Need To Know About Coronavirus--In Verse!, Rhea Paul
Community Reflections
A story in verse for young children (3-8) about coronavirus. Narrated by the author, Rhea Paul. Press the "Slide Show" button at the top of the screen, then in the menu that appears, hit "Play from Beginning" all the way to the left.
Potency And Breadth Of Human Primary Zikv Immune Sera Shows That Zika Viruses Cluster Antigenically As A Single Serotype,
2020
Oregon Health and Science University
Potency And Breadth Of Human Primary Zikv Immune Sera Shows That Zika Viruses Cluster Antigenically As A Single Serotype, Chad M. Nix, Jonathan Salberg, Felicity J. Coulter, Bettie W. Kareko, Zoe L. Lyski, Brian L. Booty, William B. Messer
OHSU-PSU School of Public Health Faculty Publications and Presentations
The recent emergence of Zika virus as an important human pathogen has raised questions about the durability and breadth of Zika virus immunity following natural infection in humans. While global epidemic patterns suggest that Zika infection elicits a protective immune response that is likely to offer long-term protection against repeat infection by other Zika viruses, only one study to date has formally examined the ability of human Zika immune sera to neutralize different Zika viruses. That study was limited because it evaluated human immune sera no more than 13 weeks after Zika virus infection and tested a relatively small number …
Ua37/2 Autism Isn't Coronavirus,
2020
Western Kentucky University
Ua37/2 Autism Isn't Coronavirus, Tom C. Hunley
Faculty/Staff Personal Papers
Poem written by WKU English professor Tom Hunley during the Covid-19 pandemic.
El Ingreso Familiar De Emergencia En Argentina: Una Exploración Cualitativa Preliminar De La Suficiencia Relativa Y El Acceso / Emergency Family Income In Argentina: A Qualitative And Preliminary Exploration Of Relative Sufficiency And Access, Seamus Mcwilliams
Independent Study Project (ISP) Collection
El gobierno argentino ha promulgado docenas de nuevas protecciones sociales y varios de apoyo financiero para limitar la devastación económica causada por la pandemia del coronavirus. Una de estas medidas es el Ingreso Familiar de Emergencia (IFE), un estímulo de 10.000 pesos dirigido a familias del sector informal. Esta población tiene poca capacidad para resistir un período indefinido sin ingreso, y simultáneamente es difícil de alcanzar con los otros programas de ingresos debido a la naturaleza de la informalidad. A través de un análisis del ecosistema de apoyo gubernamental y dos entrevistas con profesionales familiarizadas con el sector informal, este …
Non-State Actors’ Covid-19 Response In Nepal,
2020
SIT Study Abroad
Non-State Actors’ Covid-19 Response In Nepal, Jenna Mae Biedscheid
Independent Study Project (ISP) Collection
This research explores the ways in which non-state actors have responded to the COVID-19 pandemic in Nepal and the needs present in the months before drastic increases in cases began on May 11th. In doing so, it describes how social and political inequality within Nepal has caused people experiencing the most need to be left out of early phases of the COVID-19 pandemic relief effort. This research includes a literature review which situates Nepal amidst the global pandemic as well as interviews with non-state actors currently responding in Nepal. It finds that migrant workers, daily wage earners, Dalits, Janajati/Adivasi peoples, …
Optimization Of Methods For Cross-Species Infection Of Cell Cultures With Wolbachia,
2020
University of South Dakota
Optimization Of Methods For Cross-Species Infection Of Cell Cultures With Wolbachia, Sarah J. Lane
Honors Thesis
The common intracellular endosymbiont genus of bacteria called Wolbachia is of interest due to the promise it shows for playing a role in disease control. Wolbachia have a number of widely variable effects on its many species of host insects. In combination with these roles, Wolbachia largely affects reproduction and development of its host species. It can lead to feminization and cytoplasmic incompatibility (Werren et al. 2008). Furthermore, this species is vertically transmitted which means that it passes from mother to offspring (Caragata et al., 2016). In combination with its effects on reproduction, which can allow Wolbachia to propagate through …
Evidence Of The Covid-19 Virus Targeting The Cns: Tissue Distribution, Host-Virus Interaction, And Proposed Neurotropic Mechanisms,
2020
Aga Khan University
Evidence Of The Covid-19 Virus Targeting The Cns: Tissue Distribution, Host-Virus Interaction, And Proposed Neurotropic Mechanisms, Abdul Mannan Baig, Areeba Khaleeq, Usman Ali, Hira Syeda
Department of Biological & Biomedical Sciences
The recent outbreak of coronavirus infectious disease 2019 (COVID-19) has gripped the world with apprehension and has evoked a scare of epic proportion regarding its potential to spread and infect humans worldwide. As we are in the midst of an ongoing pandemic of COVID-19, scientists are struggling to understand how it resembles and differs from the severe acute respiratory syndrome coronavirus (SARS-CoV) at the genomic and transcriptomic level. In a short time following the outbreak, it has been shown that, similar to SARS-CoV, COVID-19 virus exploits the angiotensin-converting enzyme 2 (ACE2) receptor to gain entry inside the cells. This finding …
Managing The Threat Of Covid-19,
2020
CUNY New York City College of Technology
Managing The Threat Of Covid-19, Susan H. Davide, Anty Lam, Christine Macarelli
Publications and Research
Oral health professionals should remain up-to-date on Covid-19 to prevent its transmission in the dental practice.
The Current State Of Migrant Health In Morocco: Pre-And Peri-Covid-19 Pandemic,
2020
SIT Study Abroad
The Current State Of Migrant Health In Morocco: Pre-And Peri-Covid-19 Pandemic, Layla Babahaji
Independent Study Project (ISP) Collection
In the early 21st century, Morocco transitioned from being a predominantly transit migration country into both a transit and destination country for migrants. In 2013, the Moroccan government took significant steps to adapt the healthcare system in better integrating migrants in its policies. The government implemented the National Strategy on Immigration and Asylum that improved access to public health care for migrants. Recently, in March of 2020, Morocco declared a state of medical emergency due to the current COVID-19 pandemic that continues today. This paper addresses the effects of the COVID-19 pandemic on the current state of migrant health in …
Ua37/2 If This Next Apocalypse Gets Canceled Or Postponed,
2020
Western Kentucky University
Ua37/2 If This Next Apocalypse Gets Canceled Or Postponed, Tom Hunley
Faculty/Staff Personal Papers
Poem written by WKU English professor Tom Hunley during Covid-19 pandemic.
