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Articles 7651 - 7680 of 9760

Full-Text Articles in Veterinary Medicine

Mycobacterium Avium Subsp. Paratuberculosis In Veterinary Medicine, N. Beth Harris, Raul G. Barletta Jul 2001

Mycobacterium Avium Subsp. Paratuberculosis In Veterinary Medicine, N. Beth Harris, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Bacteria of the genus Mycobacterium are gram-positive, acid- fast organisms that include a number of significant human and animal pathogens. Mycobacterium avium subsp. paratuberculosis (basonym M. paratuberculosis) is the etiological agent of a severe gastroenteritis in ruminants, known as Johne’s disease. H. A. Johne and L. Frothingham initially reported the disease in Germany in 1894. However, it was not until 1910 that F. W. Trowt successfully fulfilled Koch’s postulates by growing M. paratuberculosis in the laboratory and reproducing the disease in experimentally infected cattle (46, 148). Johne’s disease is prevalent in domestic animals worldwide and has significant impact on …

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The Role Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In The Porcine Respiratory Disease Complex (Prdc), Roongroje Thanawongnuwech Jun 2001

The Role Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In The Porcine Respiratory Disease Complex (Prdc), Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Concurrent bacterial infections of the respiratory tract in porcine reproductive and respiratory syndrome virus (PRRSV)-infected pigs is a major problem for the swine industry and may involve various factors. This review manuscript discusses the role of pulmonary macrophages, which are affected by PRRSV. The macrophage's phagocytic and bactericidal ability is altered by the PRRSV infection as is pulmonary clearance. PRRSV also induces apoptosis and macrophages cell lysis leading to an increased susceptibility to secondary bacterial infection, and the syndrome called porcine respiratory disease complex (PRDC). Prevention and control strategies for these concurrent infections are briefly discussed.

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A Synchronized Ovulation Program That Improves Reproductive Efficiency In Dairy Cows, Veerasak Punyapornwithaya, Prachin Virakul, Janpen Suwimonteerabutr Jun 2001

A Synchronized Ovulation Program That Improves Reproductive Efficiency In Dairy Cows, Veerasak Punyapornwithaya, Prachin Virakul, Janpen Suwimonteerabutr

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the reproductive efficiency of lactating dairy cows by using a synchronized ovulation program (Ovsynch). Multiparous, crossbred, Holstein-Friesian cows (40-70 d postpartum; n=60) were divided into a control group (n=30) and an Ovsynch group (n=30), according to their calving dates. Cows in the control group were artificially inseminated according to the AM/PM rule following the detection of standing estrus. The Ovsynch cows were injected with 100 μg GnRH at various stages of the estrous cycle on the first day of the program. Seven days later, they were injected with 500 μg PGF2α, followed …

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The Treatment Of Canine Demodicosis With The Oral Application Of Ivermectin, Nuvee Prapasarakul, Nlin Arya, Chaiyot Tarnrattana, Supote Methiyapun, Manop Muaungyai Jun 2001

The Treatment Of Canine Demodicosis With The Oral Application Of Ivermectin, Nuvee Prapasarakul, Nlin Arya, Chaiyot Tarnrattana, Supote Methiyapun, Manop Muaungyai

The Thai Journal of Veterinary Medicine

Canine demodicosis was treated with Ivermectin (Ivomec®) given orally at a dosage of 0.6 mg/kg to seven dogs. Three of the dogs were from a city pound, the others were privately owned. Six dogs had generalized lesions and one had localized ones. A complete blood count and their biochemical profiles were monitored each month. After treatment, the dogs recovered and had no demodectic mange in the multiple skin scrapings. The duration of treatment was 5-9 weeks (mean= 6.7 weeks). No adverse drug effects were seen during treatment and all blood values remained within the normal range.

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What Is Your Diagnosis?, Pranee Tuntivanich Jun 2001

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

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Dentigerous Cyst In A Thoroughbred Colt: A Case Report, Atichat Brahmasa, Marissak Kalpravidh, Chanin Kalpravidh Jun 2001

Dentigerous Cyst In A Thoroughbred Colt: A Case Report, Atichat Brahmasa, Marissak Kalpravidh, Chanin Kalpravidh

The Thai Journal of Veterinary Medicine

This paper reports surgical resection of a dentigerous cyst from a thoroughbred colt that had persistent seromucous discharge through a small opening at the base of the right pinna for one month.Healing was uneventful after the cyst, as well as the fistulous tract, was totally removed. An aberranttooth of molar liked appearance was found within the cyst.

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Chromosomal Studies On A Bull With A Congenitally Abnormal Urethra, Duangsmorn Suwattana, Weerapong Koykul, Vivat Chavananikul Jun 2001

Chromosomal Studies On A Bull With A Congenitally Abnormal Urethra, Duangsmorn Suwattana, Weerapong Koykul, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

A six-year old bull (Bos indicus) was found to have hypospadia (congenital rupture of the ventral aspect of the penile urethra). The testes were a normal size and the penis slightly hypoplastic. Karyotype obtained from the conventional staining of chromosomes and GTG- and RBA- bandings revealed a normal make-up, of 60 XY male chromosomes. The levels of serum testoterone, estradiol and progesterone were all within the normal range. Based on the phenotypic abnormality and the karyotypic findings, this bull can be classified as a male, pseudohermaphrodite.

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Diagnostic Forum, Phiwipha Kamonrat Jun 2001

Diagnostic Forum, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

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Experimental Deer-To-Deer Transmission Of Mycobacterium Bovis, Mitchell V. Palmer, Diana L. Whipple, W. Ray Waters May 2001

Experimental Deer-To-Deer Transmission Of Mycobacterium Bovis, Mitchell V. Palmer, Diana L. Whipple, W. Ray Waters

Michigan Bovine Tuberculosis Bibliography and Database

Objective—To determine whether Mycobacterium bovis can be transmitted from experimentally infected deer to uninfected in-contact deer.

Animals—Twenty-three 6-month-old white-tailed deer.

Procedure—On day 0, M bovis (2 X 108 colony-forming units) was administered by intratonsillar instillation to 8 deer; 3 control deer received saline (0.9% NaCl) solution. Eight in-contact deer were comingled with inoculated deer from day 21. On day 120, inoculated deer were euthanatized and necropsied. On day 180, 4 in-contact deer were euthanatized, and 4 new incontact deer were introduced. On day 360, all in-contact deer were euthanatized. Rectal, oral, and nasal swab specimens and …

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Evidence For Two Mechanisms Of Amino Acid Osmolyte Release From Hippocampal Slices, R. Franco, M. E. Torres-Marquez, H. Pasantes-Morales Apr 2001

Evidence For Two Mechanisms Of Amino Acid Osmolyte Release From Hippocampal Slices, R. Franco, M. E. Torres-Marquez, H. Pasantes-Morales

School of Veterinary and Biomedical Sciences: Faculty Publications

A 30% decrease in osmolarity stimulated 3H-taurine, 3H-GABA and glutamate (followed as 3H-D-aspartate) efflux from rat hippocampal slices. 3H-taurine efflux was activated rapidly but inactivated slowly. It was decreased markedly by 100 μM 5-nitro-(3-phenylpropylamino) benzoic acid (NPPB) and 600 μM niflumic acid and inhibited strongly by tyrphostins AG18, AG879 and AG112 (25–100 μM), suggesting a tyrosine kinase-mediated mechanism. Hyposmolarity activated the mitogen-activated protein kinases (MAPK) extracellular-signal-related kinase-1/2 (ERK1/ERK2) and p38, but blockade of this reaction did not affect 3H-taurine efflux. Hyposmosis also activated phosphatidyl-inositide 3-kinase (PI3K) and its prevention by wortmannin (100 nM) essentially abolished …

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Persistent Bovine Viral Diarrhea Virus Infection In Us Beef Herds, T. E. Wittum, Dale M. Grotelueschen, K. V. Brock, William G. Kvasnicka, J. G. Floyd, Clayton L. Kelling, K. G. Odde Apr 2001

Persistent Bovine Viral Diarrhea Virus Infection In Us Beef Herds, T. E. Wittum, Dale M. Grotelueschen, K. V. Brock, William G. Kvasnicka, J. G. Floyd, Clayton L. Kelling, K. G. Odde

School of Veterinary and Biomedical Sciences: Faculty Publications

In the summer of 1996, we screened 18,931 calves in 128 beef herds located in five US states for persistent bovine viral diarrhea virus (BVDV) infection. Of these, 76 herds were randomly selected from the client database of collaborating veterinary practices, and 52 herds were suspected by the collaborating veterinarians to have BVDV infection based on history or clinical signs. Serum was obtained from each calf in the cooperating herds prior to 4 months of age and tested for the presence of BVDV by microtiter virus isolation. Information about each of the herds (including management practices, vaccination history, and breeding- …

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Capitalism And The Value Of Life: Foot-And-Mouth And The Culling Of Herds, Ibpp Editor Apr 2001

Capitalism And The Value Of Life: Foot-And-Mouth And The Culling Of Herds, Ibpp Editor

International Bulletin of Political Psychology

This article describes some psychological implications of culling as an intervention to help combat the spread of foot-and-mouth disease.

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Septicemia Associated With Stenotrophomonas Maltophilia In A West African Dwarf Crocodile (Osteolaemus Tetraspis Subsp. Tetraspis), N. Beth Harris, Douglas G. Rogers Apr 2001

Septicemia Associated With Stenotrophomonas Maltophilia In A West African Dwarf Crocodile (Osteolaemus Tetraspis Subsp. Tetraspis), N. Beth Harris, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

A 17-year-old male captive West African dwarf crocodile (Osteolaemus tetraspis subsp. tetraspis) died 1 month after fighting with a penmate. Abrasions were present on the head and mandible. Necropsy revealed a vegetative valvular lesion of the left atrioventricular valve, miliary foci of necrosis in the endocardium and myocardium, multiple duodenal and rectal ulcers, and serous atrophy of body fat. Stenotrophomonas maltophilia was isolated in pure culture from lung, liver, and kidney. Gram-negative bacilli were seen histologically in the valvular lesion and in foci of necrosis in the myocardium, liver, spleen, pancreas, kidney, and intestine. Septic thrombi in multiple …

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Region Of Herpes Simplex Virus Type 1 Latency-Associated Transcript Sufficient For Wild-Type Spontaneous Reactivation Promotes Cell Survival In Tissue Culture, Melissa Inman, Guey-Chuen Perng, Gail A. Henderson, Homayon Ghiasi, Anthony B. Nesburn, Steven L. Wechsler, Clinton J. Jones Apr 2001

Region Of Herpes Simplex Virus Type 1 Latency-Associated Transcript Sufficient For Wild-Type Spontaneous Reactivation Promotes Cell Survival In Tissue Culture, Melissa Inman, Guey-Chuen Perng, Gail A. Henderson, Homayon Ghiasi, Anthony B. Nesburn, Steven L. Wechsler, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

The latency-associated transcript (LAT) is the only abundant herpes simplex virus type 1 (HSV-1) transcript expressed during latency. In the rabbit eye model, LAT null mutants do not reactivate efficiently from latency. We recently demonstrated that the LAT null mutant dLAT2903 induces increased levels of apoptosis in trigeminal ganglia of infected rabbits compared to LAT+ strains (G.-C. Perng, C. Jones, J. Ciacci-Zarella, M. Stone, G. Henderson, A. Yokht, S. M. Slanina, F. M. Hoffman, H. Ghiasi, A. B. Nesburn, and C. S. Wechsler, Science 287:1500–1503, 2000).The same study also demonstrated that a plasmid expressing LAT nucleotides 301 to …

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Trends. The Psycho-Politics Of Foot In Mouth On Foot In Mouth: Pigs, Sheep, Cows, Politicians On The Animal Farm, Ibpp Editor Mar 2001

Trends. The Psycho-Politics Of Foot In Mouth On Foot In Mouth: Pigs, Sheep, Cows, Politicians On The Animal Farm, Ibpp Editor

International Bulletin of Political Psychology

This article discusses agricultural policy in the United Kingdom regarding foot and mouth disease of cloven-hoofed animals.

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A Comparison Of Resazurin Test Scores On Milk, After 1 And 3 Hours And Comparing It With Total Bacteria, Coliform And Somatic Cell Counts, Sophat Chawalkul, Witaya Suriyasathaporn Mar 2001

A Comparison Of Resazurin Test Scores On Milk, After 1 And 3 Hours And Comparing It With Total Bacteria, Coliform And Somatic Cell Counts, Sophat Chawalkul, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The present study was conducted to compare results of a resazurin test on milk, scoring it after 1 hour and 3 hours and relating it to total bacterial count, coliform count and somatic cell counts. Milk samples from the bulk tanks of 337 dairy farms in Amphur Muang and Kumpangsan, Nakhon Pathom Province, were collected during October to December, 1998. Results from multiple regression analyses showed that the resazurin test scored 1 hour later was associated only with the total bacterial count (p < 0.05), but after 3 hours associations with total bacteria, coliform and somatic cell counts were seen (p < 0.05). We conclude that a resazurin test scored after 3 hours gave a better result than the test scored after 1 hour when judging total bacteria, coliform and somatic cell counts

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Ultrasound Diagnosis, Phiwipha Kamonrat Mar 2001

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

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Ornithobacterium Rhinotracheale Infection In Birds, Niwat Chansiripornchai, Piyarat Subhachalat Mar 2001

Ornithobacterium Rhinotracheale Infection In Birds, Niwat Chansiripornchai, Piyarat Subhachalat

The Thai Journal of Veterinary Medicine

Ornithobacterium rhinotracheale is a recently discovered bacterium of the rRNA superfamily V, first named in 1994. The first occurence was reported in wild birds and later shown to be widespread worldwide in commercial poultry. It has not been reported in Thailand. The bacterium causes important ecomonic losses in the poultry industry. O. rhinotracheale can affect almost all avian species and is associated with respiratory diseases. Airsacculitis and pneumonia are the most common features of infection. These clinical signs are aggravated by other factors, especially respiratory viruses and bacterial infections. Infection can be transmitted horizontally by aerosol droplets and also vertically …

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The Use Of Fresh, Salt-Stored And Frozen, Immature And Mature Oocytes, To Assess The Penetration Ability Of Porcine Spermatozoa, Adisorn Adirekthaworn, Mongkol Techakumphu, Sudson Sirivaidyapong, Saroch Gnamkum Mar 2001

The Use Of Fresh, Salt-Stored And Frozen, Immature And Mature Oocytes, To Assess The Penetration Ability Of Porcine Spermatozoa, Adisorn Adirekthaworn, Mongkol Techakumphu, Sudson Sirivaidyapong, Saroch Gnamkum

The Thai Journal of Veterinary Medicine

The objective of this study was to compare the penetration rate of sperm, collected from two Landrace Boars A and B, into fresh, salt-stored and frozen, immature and matured oocytes. The first experiment, performed on immature oocytes, revealed that the penetration rates for the three groups of oocytes was from Boar A 59.6%(59/99), 78.1%(75/96) and 77.8%(77/99) (p<0.05) and the number of sperm penetration each oocyte was 2.79±0.42, 2.97±0.29 and 2.29±0.26 respectively (p>0.05). While for Boar B, the percentages were 65.3%(62/95), 76.8%(73/95) and 77.8%(77/99) with the number of sperm per oocyte being 2.25±0.28, 3.63±0.42 and 2.57±0.36. The highest values were found in the salt-stored group (p<0.05). The second experiment involved sperm penetration into in vitro mature oocytes. No differences were found for the sperm penetration rate and the number of sperm per oocyte using semen from Boar A which were 85.1%(74/87), 86.1%(56/65) and 89.1%(79/88) and 13.87±1.45, 17.69±2.61 and 14.45±1.75. While for Boar B, the lowest penetration rate was found when using fresh oocyte, lower than the two other groups; 52.6%(50/95), 67.3%(64/95) and 69.1%(67/ 97)(p<0.05). The number of sperm entering each oocyte was also inferior in the fresh oocytes than in salt-stored and frozen ones; 1.55±0.31, 2.80±0.35 and 2.87±0.40 (p<0.05) respectively. The conclusion that can be drawn from the experiment is that a) there are significant differences in the sperm penetration ability of different the boars used and b) the type of oocytes; immature vs. mature and fresh vs. salt-stored vs. frozen influenced the results that were obtained. It maybe possible to apply such an in vitro penetration assay to determine boar semen fertility under field conditions.

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The Effect Of Methylparathion On Cholinesterase Activity In Cockle (Anadara Granosa L.) Muscle Tissues, Yanin Limpanon, Sukanya Phalitakul, Griangyut Tapcharoen, Janenuj Wongtavatchai, Piyarat Subhachalat Mar 2001

The Effect Of Methylparathion On Cholinesterase Activity In Cockle (Anadara Granosa L.) Muscle Tissues, Yanin Limpanon, Sukanya Phalitakul, Griangyut Tapcharoen, Janenuj Wongtavatchai, Piyarat Subhachalat

The Thai Journal of Veterinary Medicine

The effect of methylparathion on cholinesterase activity in foot muscle tissues of cockle were evaluated. Ninety - five healthy cockle were randomly divided into control and experimental groups. The experimental group was exposed to 10 ppb of methylparathion for 90 hours. Measurements of cholinesterase activity revealed increasing enzyme activity in foot muscle (p < 0.05) in the exposed animals while clinical observations showed no differences between the groups. The results indicate that methylparathion affects the observed activity of cholinesterase in cockle. However, it should be noted that the data do not correlate with those of previous studies reported in other aquatic animal species.

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The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura Mar 2001

The Detection Of Equine Herpesvirus Antigen In An Aborted Foal Using Immunohistochemistry : A Case Report, Nopadon Pirarat, Wijit Kiatipattanasakul, Theerawat Tharasanit, Pattama Ritruechai, Umi Une, Yasuo Nomura

The Thai Journal of Veterinary Medicine

Formalin fixed tissues from an aborted foal which had been diagnosed as having Equine Herpesvirus (EHV) infection, on histopathological criteria, were examined for EHV antigen using immunohistochemistry. EHV antigen was found in the focal necrotic area of the liver, especially in hepatocytes and Kupffer cells. Intranuclear and intracytoplasmic, immuno-positive labelings were also prominent in bronchiolar epithelial cells. The method allowed the visualization of the antigenic site distribution and their subsequent correlation with histopathological changes.

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Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Phiwipa Kamonrat Mar 2001

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Phiwipa Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

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Infections With Ehrlichia Chaffeensis And Ehrlichia Ewingii In Persons Coinfected With Human Immunodeficiency Virus, Christopher D. Paddock, Scott M. Folk, G. Merrill Shore, Linda J. Machado, Mark M. Huycke, Leonard N. Slater, Allison M. Liddell, Richard S. Buller, Gregory A. Storch, Thomas P. Monson, David Rimland, John W. Sumner, Joseph Singleton, Karen C. Bloch, Yi-Wei Tang, Steven M. Standaert, James E. Childs Jan 2001

Infections With Ehrlichia Chaffeensis And Ehrlichia Ewingii In Persons Coinfected With Human Immunodeficiency Virus, Christopher D. Paddock, Scott M. Folk, G. Merrill Shore, Linda J. Machado, Mark M. Huycke, Leonard N. Slater, Allison M. Liddell, Richard S. Buller, Gregory A. Storch, Thomas P. Monson, David Rimland, John W. Sumner, Joseph Singleton, Karen C. Bloch, Yi-Wei Tang, Steven M. Standaert, James E. Childs

Zoonotics and Wildlife Disease: Publications

The clinical course and laboratory valuation of 21 patients coinfected with human immunodeficiency virus (HIV) and Ehrlichia chaffeensis or Ehrlichia ewingii are reviewed and summarized, including 13 cases of ehrlichiosis caused by E. chaffeensis, 4 caused by E. ewingii, and 4 caused by either E. chaffeensis or E. ewingii. Twenty patients were male, and the median CD4+ T lymphocyte count was 137 cells/μL. Exposures to infecting ticks were linked to recreational pursuits, occupations, and peridomestic activities. For 8 patients, a diagnosis of ehrlichiosis was not considered until ≥4 days after presentation. Severe manifestations occurred more frequently …

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Prospective Assesment Of The Etiology Of Acute Febrile Illness After A Tick Bite In Slovenia, Stanka Lotric-Furlan, Miroslav Petrovec, Tatjana Avsic-Zupanc, William L. Nicholson, John W. Sumner, James E. Childs, Franc Strle Jan 2001

Prospective Assesment Of The Etiology Of Acute Febrile Illness After A Tick Bite In Slovenia, Stanka Lotric-Furlan, Miroslav Petrovec, Tatjana Avsic-Zupanc, William L. Nicholson, John W. Sumner, James E. Childs, Franc Strle

Zoonotics and Wildlife Disease: Publications

A prospective study established the etiology of febrile illnesses in residents of Slovenia that occurred within 6 weeks after a tick bite. A combination of laboratory and clinical criteria identified 64 (49.2%) of 130 patients as having confirmed, probable, or possible cases of tickborne disease during 1995 and 1996. Of the 130 patients, 36 (27.7%) had laboratory evidence of tickborne encephalitis, all of whom had clinically confirmed disease. Evidence of infection with Borreliab urgdorfersie sensu lato was identified in 26 patients; 10 (7.7%) had confirmed Lyme borreliosis. Of 22 patients with evidence of Ehrlichiap hagocytophila infection, 4 (3.1%) had confirmed …

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Analysis Of Risk Factors For Fatal Rocky Mountain Spotted Fever: Evidence For Superiority Of Tetracyclines For Therapy, Robert C. Holman, Christopher D. Paddock, Aaron T. Curns, John W. Krebs, Jennifer H. Mcquiston, James E. Childs Jan 2001

Analysis Of Risk Factors For Fatal Rocky Mountain Spotted Fever: Evidence For Superiority Of Tetracyclines For Therapy, Robert C. Holman, Christopher D. Paddock, Aaron T. Curns, John W. Krebs, Jennifer H. Mcquiston, James E. Childs

Zoonotics and Wildlife Disease: Publications

Epidemiologic and clinical characteristics of fatal and nonfatal cases of Rocky Mountain spotted fever (RMSF) were compared to identify risk factors for death caused by this disease. Confirmed and probable RMSF cases reported through US national surveillance for 1981- 1998 were analyzed. Among 6388 RMSF patients, 213 died (annual case-fatality rate, 3M; range, 4.9 % in 1982 to 1.1 % in 1996). Use of tetracycline-class antibiotics for treatment of RMSF increased significantly in the 1990s, compared with use in the 1980s. Older patients, patients treated with chloramphenicol only, patients for whom tetracycline antibiotics were not the primary therapy, and patients …

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Prp-C And Prp-Sc At The Fetal-Maternal Interface, Wenbin Tuo, Dongyue Zhuang, Donald P. Knowles, William P. Cheevers, Man-Sun Sy, Katherine I. O'Rourke Jan 2001

Prp-C And Prp-Sc At The Fetal-Maternal Interface, Wenbin Tuo, Dongyue Zhuang, Donald P. Knowles, William P. Cheevers, Man-Sun Sy, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Scrapie is a naturally occurring prion (PrP) disease causing a fatal neurodegenerative disorder in sheep and goats. Previous studies suggest that scrapie is transmitted naturally through exposure to the scrapie agent in wasted placentas of infected ewes. This study determined the distribution and biochemical properties of PrP cellular (PrP-C) and the distribution of PrP scrapie (PrP-Sc) in reproductive, placental, and selected fetal tissues and fetal fluids in sheep. Glycosylated, N-terminally truncated, proteinase K-sensitive PrP-C with apparent molecular masses of 23–37 kDa was present in reproductive, placental, and fetal tissues and fetal fluids. PrP-C was low or undetectable in intercotyledonary chorioallantois, …

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Immunohistochemical Detection Of Scrapie Prion Proteins In Clinically Normal Sheep In Pennsylvania, Hyun Kim, Katherine I. O'Rourke, Mark Walter, H. Graham Purchase, John Enck, Tae Kyun Shin Jan 2001

Immunohistochemical Detection Of Scrapie Prion Proteins In Clinically Normal Sheep In Pennsylvania, Hyun Kim, Katherine I. O'Rourke, Mark Walter, H. Graham Purchase, John Enck, Tae Kyun Shin

Zoonotics and Wildlife Disease: Publications

Following diagnosis of scrapie in a clinically suspect Suffolk sheep, 7 clinically normal flockmates were purchased by the Pennsylvania Department of Agriculture to determine their scrapie status using an immunohistochemical procedure. Two of the 7 euthanized healthy sheep had positive immunohistochemical staining of the prion protein of scrapie (PrP-Sc) in their brains, nictitating membranes, and tonsils. The PrP-Sc was localized in the areas of the brain where, histopathologically, there was neurodegeneration and astrocytosis. The PrP-Sc occurred within germinal centers of the affected nictitating membranes and tonsils and was located in the cytoplasm of the dendrite-like cells, lymphoid cells, and macrophages. …

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Preliminary Findings On The Experimental Transmission Of Chronic Wasting Disease Agent Of Mule Deer To Cattle, Amir N. Hamir, Randall C. Cutlip, Janice M. Miller, Elizabeth S. Williams, Mick J. Stack, Michael W. Miller, Katherine I. O'Rourke, Melanie J. Chaplin Jan 2001

Preliminary Findings On The Experimental Transmission Of Chronic Wasting Disease Agent Of Mule Deer To Cattle, Amir N. Hamir, Randall C. Cutlip, Janice M. Miller, Elizabeth S. Williams, Mick J. Stack, Michael W. Miller, Katherine I. O'Rourke, Melanie J. Chaplin

Zoonotics and Wildlife Disease: Publications

To determine the transmissibility of chronic wasting disease (CWD) to cattle and to provide information about clinical course, lesions, and suitability of currently used diagnostic procedures for detection of CWD in cattle, 13 calves were inoculated intracerebrally with brain suspension from mule deer naturally affected with CWD. Between 24 and 27 months postinoculation, 3 animals became recumbent and were euthanized. Gross necropsies revealed emaciation in 2 animals and a large pulmonary abscess in the third. Brains were examined for protease-resistant prion protein (PrPres) by immunohistochemistry and Western blotting and for scrapie-associated fibrils (SAFs) by negative-stain electron microscopy. Microscopic …

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Arbovirus Infection Increases With Group Size, Charles R. Brown, Nicholas Komar, Sunita B. Quick, Rajni A. Sethi, Nicholas A. Panella, Mary Bromberger Brown, Martin Pfeffer Jan 2001

Arbovirus Infection Increases With Group Size, Charles R. Brown, Nicholas Komar, Sunita B. Quick, Rajni A. Sethi, Nicholas A. Panella, Mary Bromberger Brown, Martin Pfeffer

Zoonotics and Wildlife Disease: Publications

Buggy Creek (BCR) virus is an arthropod-borne alphavirus that is naturally transmitted to its vertebrate host the cliff swallow (Petrochelidon pyrrhonota) by an invertebrate vector, namely the cimicid swallow bug (Oeciacus vicarius). We examined how the prevalence of the virus varied with the group size of both its vector and host. The study was conducted in southwestern Nebraska where cliff swallows breed in colonies ranging from one to 3700 nests and the bug populations at a site vary directly with the cliff swallow colony size. The percentage of cliff swallow nests containing bugs infected with BCR …

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Department Of Veterinary And Biomedical Sciences: 2001 Annual Report, John A. Schmitz Jan 2001

Department Of Veterinary And Biomedical Sciences: 2001 Annual Report, John A. Schmitz

School of Veterinary and Biomedical Sciences: Information and History

Twenty Departmental students completed their Bachelor of Science in Agricultural Science degrees in 2001, nineteen in the Veterinary Science Major and one in the Veterinary Technologist Major. Twenty-five Nebraska residents were admitted into the College of Veterinary Medicine at Kansas State University, twelve of them having completed all or part of their pre-veterinary studies at UNL. Seven students completed their Master of Science degree in Veterinary Science. Enrollment in the Distance Education MS in Veterinary Science Master of Science, Distance Education Program increased to seven students, with several applications still being processed or expected. Three new Distance Education courses were …

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