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Articles 1 - 30 of 68
Full-Text Articles in Veterinary Medicine
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan
Turkish Journal of Veterinary & Animal Sciences
Salmonellosis caused by Salmonella agents is the second most common zoonotic infection in humans. In recent years, Salmonella's increasing antimicrobial resistance (AMR) has been a concern. The major transmission route of Salmonella is consumption of contaminated poultry products. Therefore, monitoring of antimicrobial resistance in chicken-originated Salmonella is critically important. This study investigated AMR in four commonly isolated Salmonella serovars from chickens, namely Salmonella Enteritidis (S. Enteritidis), Salmonella Infantis (S. Infantis), Salmonella Kentucky (S. Kentucky), and Salmonella Typhimurium (S. Typhimurium). A total of 133 isolates were examined by phenotypic and genotypic AMR characterization. Resistance to 14 different antimicrobials and eight …
A Surveillance For Avian Coronavirus Infectious Bronchitis Virus, Infectious Laryngotracheitis Virus, Avian Metapneumovirus, And Avian Reovirus In Poultry Flocks With Respiratory Signs In Türkiye, Özge Ardiçli, Serpi̇l Kahya Demi̇rbi̇lek, Fethi̇ye Çöven, Kami̇l Tayfun Carli
A Surveillance For Avian Coronavirus Infectious Bronchitis Virus, Infectious Laryngotracheitis Virus, Avian Metapneumovirus, And Avian Reovirus In Poultry Flocks With Respiratory Signs In Türkiye, Özge Ardiçli, Serpi̇l Kahya Demi̇rbi̇lek, Fethi̇ye Çöven, Kami̇l Tayfun Carli
Turkish Journal of Veterinary & Animal Sciences
In this study, avian coronavirus infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian metapneumovirus (AMPV), and avian reovirus (ARV) were evaluated in broiler and layer flocks. For this purpose, tracheal swabs from 48 broiler and 45 layer flocks with respiratory signs were inoculated SPF embryonated chicken eggs for virus isolation. The viruses were identified by real-time PCR. Results showed that the most common virus in both broiler and layer farms was IBV with incidence rates of 58.33% and 46.67%, respectively. ILTV, AMPV, and ARV incidences in the samples were found to be 22.22%, 13.33%, and 4.44% in layer flocks …
A Seroepidemiological Investigation On Major Viral And Bacterial Pathogens In Small-Scale Chicken Flocks In The Mekong Delta Region Of Vietnam, Nguyen Thi Bich Van, Nguyen Van Cuong, Nguyen Thi Nhung, Nguyen Thi Phuong Yen, Le Vy, Bach Tuan Kiet, Nguyen Van Hoang, Vo Be Hien, Ho Thi Viet Thu, Niwat Chansiripornchai, Guy E. Thwaites, Marc Choisy, Juan Carrique-Mas
A Seroepidemiological Investigation On Major Viral And Bacterial Pathogens In Small-Scale Chicken Flocks In The Mekong Delta Region Of Vietnam, Nguyen Thi Bich Van, Nguyen Van Cuong, Nguyen Thi Nhung, Nguyen Thi Phuong Yen, Le Vy, Bach Tuan Kiet, Nguyen Van Hoang, Vo Be Hien, Ho Thi Viet Thu, Niwat Chansiripornchai, Guy E. Thwaites, Marc Choisy, Juan Carrique-Mas
The Thai Journal of Veterinary Medicine
Small-scale commercial chicken farming represents an important source of income to rural households in the Mekong Delta region of Vietnam. A wide range of vaccines are currently administered to flocks, but is not based on empirical knowledge on pathogen circulation. We collected serum samples and vaccination data from a random sample of 267 small-scale native chicken flocks in Dong Thap to determine serological profiles by ELISA against 8 pathogens: Avian Influenza (AI), Chicken Anaemia virus (CAV), Infectious Bursal Disease (IBD) virus, Infectious Bronchitis virus (IBV), Newcastle Disease virus (NDV), O. rhinotracheale (ORT), P. multocida (PM) and M. gallisepticum (MG). The …
Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao
Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao
Turkish Journal of Veterinary & Animal Sciences
Heat stress is a lethal threat to chickens. This study investigated the protective effect of a Ginkgo biloba extract EGB761 against heat stress in chickens in vivo. A total of 200 one-day-old hens were separated randomly into control (Con), heat stress (HS), 0.1% EGB administration and heat stress (0.1% EGB+HS), 0.3% EGB administration and heat stress (0.3% EGB+HS), and 0.6% EGB administration and heat stress (0.6% EGB+HS) groups. After EGB761 administration for 45 days, ten chickens were selected randomly, assigned to one of the designated groups, and exposed to a one-time heat stress condition of 38 ± 1 °C for …
Humoral Immunity Provided By A Novel Infectious Bronchitis Vaccine Supplemented Bybacterium-Like Particles (Blps), Emadoddin Tohidi, Abolfazl Ghaniei, Hamid Farzin, Alireza Haghparast
Humoral Immunity Provided By A Novel Infectious Bronchitis Vaccine Supplemented Bybacterium-Like Particles (Blps), Emadoddin Tohidi, Abolfazl Ghaniei, Hamid Farzin, Alireza Haghparast
Turkish Journal of Veterinary & Animal Sciences
Infectious bronchitis (IB) is a notable disease of poultry flocks that results in economic loss. As a consequence of the presence of various IB virus (IBV) serotypes, control strategies, such as vaccination, should be replaced by provide broad protective immunity against the disease to date. Gram-positive enhancer matrix particles, or so-called bacterium like particles (BLPs), obtained from the bacterium Lactococcus lactis (L. lactis), have demonstrated an adjuvancy effect by providing demanding mucosal and humoral immune responses, as well as a protective cellular immunity whenever delivered admixed with a vaccine via intranasal or intraocular administration. In this study, for the first …
Effect Of In-Bag Carcass Decontamination Method On Shelf Life Of Whole Chicken Carcassespackaged In Plastic Bags, İbrahi̇m Alper Uysal, Gökhan Kürşad İnci̇li̇, Ömer Çakmak, Mehmet Çalicioğlu
Effect Of In-Bag Carcass Decontamination Method On Shelf Life Of Whole Chicken Carcassespackaged In Plastic Bags, İbrahi̇m Alper Uysal, Gökhan Kürşad İnci̇li̇, Ömer Çakmak, Mehmet Çalicioğlu
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to investigate the changes in the microbiological and sensorial attributes of fresh chicken carcasses immersed in decontamination solutions, followed by packaging in plastic bags in which decontamination solution was added during storage at 4 °C. The samples were taken on days 0, 3, 7, and 10 for microbiological and sensory attributes. Results showed that there was a limited antimicrobial effect on microbial flora in the treatment groups within the first 3 days of storage compared to the control group. However, the difference between the groups completely disappeared on days 7 and 10. The changes in the …
Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili
Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili
Graduate Theses and Dissertations
Mineral oils and metal salts are commonly used as adjuvants to enhance acquired immunity. Recently, monoclonal antibodies (MAbs) and recombinant peptides agonist CD40 receptor have shown remarkable promise for induction of rapid and robust immune responses. Limitations of this approach MAb production costs and multiple administrations due to anti-MAb immune responses. Here we demonstrate the development of a unique and sophisticated DNA aptamer-based alternative for CD40-directed delivery of universal antigens as an alternative in chickens, and potentially other vertebrate species. This receptor, expressed by antigen-presenting cells, acts as a costimulatory molecule for activated T helper lymphocytes. After initially selecting for …
Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan
Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the efficacy of the recombinant herpes virus of turkey-Newcastle disease virus (rHVT-NDV) vaccine against Newcastle disease virus (NDV) challenged in 14 days old broiler chickens. One hundred chickens were divided into 5 groups of 20 chickens each. Group 1 chickens received live NDV vaccine via the intra-nasal route. Group 2 chickens received rHVT-NDV vaccine by subcutaneous injection. Group 3 chickens, received both live NDV and rHVT-NDV vaccine. Groups 4 and 5 chickens did not receive any vaccine and served as positive and negative control groups. At 14 days old, all the chickens …
Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu
Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu
The Thai Journal of Veterinary Medicine
The purpose of this study was to evaluate the effect of a rapid heat-treatment method on Thai QX-like infectious bronchitis virus (IBV) and the efficacy on protection from the virus after heat treatment. QX-like IBV isolate THA46 was incubated at 56 oC. The incubated virus was collected every 5 min for up to 1 h and then they were inoculated into embryonated chicken eggs. At 6 days post-inoculation, allantoic fluid was harvested from the embryonated eggs which were inoculated with virus of the longest heat-treatment time that induced lesions in the embryos. That allantoic fluid was used in a subsequent …
Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina
Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina
Turkish Journal of Veterinary & Animal Sciences
The present study was conducted to evaluate the in vivo efficacy of pumpkin seeds as an alternative natural anthelmintic for chickens. Ninety Philippine Jolo native chickens of mixed sexes, aged 4?5 months and weighing 1?2 kg, were randomly distributed into three treatment groups with 30 chickens per group. Control group A was fed basic mash feed, group B received feed mixed with ground pumpkin seeds (2 g/bird per day), and group C received mebendazole-medicated feed (30 mg/kg body weight). Fifteen randomly selected chickens from each group were euthanized and necropsied before treatment, and the remaining fifteen in each group were …
Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri
Genetic Analysis Of Iranian Mycoplasma Gallisepticum Isolates Using Gene-Targeted Sequencing Of Complete Pvpa Gene, Naghmeh Kabiri, Mohsen Bashashati, Mehdi Vasfi Marandi, Mansour Banani, Fereshteh Sabouri
Turkish Journal of Veterinary & Animal Sciences
This study aims to evaluate Mycoplasma gallisepticum (MG) genetic diversity through the complete sequencing of the pvpA gene among six MG isolates present in industrial poultry from 2015 to 2016. The genetic comparison of these six isolates showed similarities of over 94% and 92% in terms of nucleotide and amino acid sequences, respectively. The size of the PvpA protein in the studied isolates included three different sizes with 361, 364, and 366 amino acids. The carboxy-terminus of the studied PvpA proteins contained 11 tetrapeptides of P-R-P-M/Q/N motifs with truncation in the proline-rich region. Based on the BLAST analysis, the pvpA …
Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan
Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This study determined the efficacy of recombinant HVT- NDV ( rHVT- NDV) vaccine simultaneously vaccinated with live vaccine at 1 day old and a booster vaccination with live vaccine at 10 days old against Asiatic, Genotype VII NDV in broiler chickens. The chickens were divided into 5 groups. The chickens in groups 1, 2 and 3 were vaccinated with rHVT- NDV vaccine and different live vaccines at 1 day old, and then finally received a booster vaccination at 10 days old. The chickens in groups 4 and 5 served as the positive and negative control groups, respectively. The chickens in …
Immunostimulatory Effects Of Antigen-Conjugated Inp/Zns Quantum Dot Nanoparticles In An Avian Model, Christopher Lyle
Immunostimulatory Effects Of Antigen-Conjugated Inp/Zns Quantum Dot Nanoparticles In An Avian Model, Christopher Lyle
Graduate Theses and Dissertations
Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and …
Development Of Indirect Enzyme-Linked Immunosorbent Assay For Antibody Detection Against Avibacterium Paragallinarum, Panchita Hongprasertkul, Wisanu Wanasawaeng, Niwat Chansiripornchai
Development Of Indirect Enzyme-Linked Immunosorbent Assay For Antibody Detection Against Avibacterium Paragallinarum, Panchita Hongprasertkul, Wisanu Wanasawaeng, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Indirect enzyme-linked immunosorbent assay (I-ELISA) for antibody detection against A. paragallinarum serovars A, B and C was developed in separated plates. One hundred Babcock 308 female layer chickens were randomly divided into five groups of 20 each. Groups 1, 2, 3 and 4 were different positive control groups of immunized chickens with commercial trivalent mineral oil vaccine, and prepared bacterins of Avibacterium paragallinarum serovars A (221), B (0222) and C (Modesto), respectively. The chickens in Group 5 were assigned as a negative control. Positive and negative control sera from the chickens in Groups 1-5 at 2 weeks after the second …
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens, Sirin Theerawatanasirikul, Nunyarat Koomkrong, Autchara Kayan, Chaiwat Boonkaewwan
Intestinal Barrier And Mucosal Immunity In Broilers, Thai Betong, And Native Thai Praduhangdum Chickens, Sirin Theerawatanasirikul, Nunyarat Koomkrong, Autchara Kayan, Chaiwat Boonkaewwan
Turkish Journal of Veterinary & Animal Sciences
The intestinal barrier includes physical and chemical components for preventing the invasion of pathogenic and toxic agents. The aim of this study was to investigate small intestinal morphology, antimicrobial peptides, and tight junction (TJ) distribution among broilers, Thai Betong chickens, and native Thai Praduhangdum chickens. Intestinal samples from 40 chickens of each breed were collected. The results of the histological and morphological examination revealed that the duodenum of all breeds had the maximum villus height compared to the other parts. The intestinal tract of both Betong and Praduhangdum chickens was low in the number of mucin and goblet cells. In …
The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen
The Protective Efficacy Of Immunoglobulin Y From Immunized Chickens Against Salmonella Infections In Mice, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Gökçenur Sani̇oğlu Gölen
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those …
Protective Efficacy Of Live Lasota Strain Newcastle Disease Virus Vaccine In Layer-Type Chickens, Jiroj Sasipreeyajan, Phornphisut Areeraksakul, Somkid Khanda
Protective Efficacy Of Live Lasota Strain Newcastle Disease Virus Vaccine In Layer-Type Chickens, Jiroj Sasipreeyajan, Phornphisut Areeraksakul, Somkid Khanda
The Thai Journal of Veterinary Medicine
The purpose of this study was to evaluate the protective efficacy of live LaSota strain Newcastle disease virus (NDV) vaccine administered with different routes against a NDV challenge in layer-type chickens. Fifty male layer-type chickens were divided into 3 groups. There were 20 chickens/group for the vaccinated groups (Groups 1 and 2) and 10 chickens for the non-vaccinated control group (Group 3). Chickens in Groups 1 and 2 received the vaccine individually at 4 weeks old by eye drop and oral drop, respectively. All chickens received NDV challenge individually by oral drop at 7 weeks old or 3 weeks post-vaccination …
Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi
Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi
The Thai Journal of Veterinary Medicine
The aflatoxin effect on humoral and mucosal immunity against infectious bronchitis (IB) vaccine was studied in 225 one-day-old chicks. The chicks were divided in 3 equal groups. Treatment groups included group A: chickens fed a basal diet, and group B: chickens fed 3 ppm productive aflatoxin in a basal diet. The chickens in groups A and B were vaccinated against IB (at one and 10 days old) by H120 live attenuated vaccine. For group C, chickens did not receive aflatoxin and were not vaccinated against IB. All chickens received experimental diets from 3 to 28 days old, continuously. At 28 …
Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Turkish Journal of Veterinary & Animal Sciences
Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it has relatively poor oral bioavailability. This study was conducted to compare the bioavailability (BA) and main pharmacokinetic (PK) parameters of TLM after oral administration of tilmicosin phosphate (TLM-PH) and three newly prepared lipid nanoparticles (LNPs) of TLM including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Sixty broiler chickens were divided into eight groups. In four treatment groups (n = 10), each bird was given a single oral dose (20 mg/kg) of a TLM formulation after overnight fasting, and in four …
Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan
Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This study investigated the level of protection generated by vaccination with a live attenuated infectious bronchitis virus (IBV) vaccine, H120 strain against variant genotype IBV isolated in Thailand. Sixty 1-day-old female broiler chickens were randomly divided into 4 groups of 15 chickens each. The chickens in Group 1 were vaccinated at 1 day old, whereas the chickens in Group 2 were vaccinated at 7 days old. Groups 3 and 4 did not receive IBV vaccine and served as positive and negative control groups, respectively. At 21 days of age, the chickens in Groups 1-3 were individually challenged with 104.8 EID50 …
A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong
A Novel Approach For Determination Of Chicken Sexing At An Early Stage Of Development By Using Loop-Mediated Isothermal Amplification Method, Sung Woo Kim, Jin Seok Choi, Neelesh Sharma, Yeoung Gyu Ko, Yoon Jung Do, Mijeong Byun, Hwan Hoo Seong, Soo Bong Park, Dong Kee Jeong
Turkish Journal of Veterinary & Animal Sciences
The monomorphism of newly hatched chicks is a major hurdle for poultry farmers at the time of sexing. The traditional sexing techniques are inaccurate and time-consuming. Therefore, the present study aimed to develop a loop-mediated isothermal amplification (LAMP)-based sex determination method to obtain a higher separation accuracy rate as compared to conventional methods. LAMP is a simple, quick, and relatively inexpensive diagnostic tool. Genomic DNA was extracted from the blood samples of Leghorn and Korean native chicks for LAMP. Two sets of primer were designed, consisting of four isothermal amplification primers in each, to amplify the female sex-specific sequence (HUR0417) …
Efficacy Of Live Infectious Bronchitis Vaccine Programs Against Infection By Qx-Like Strain Of Infectious Bronchitis Virus, Ekasingh Sarueng, Wisanu Wanasawaeng, Jiroj Sasipreeyajan, Niwat Chansiripornchai
Efficacy Of Live Infectious Bronchitis Vaccine Programs Against Infection By Qx-Like Strain Of Infectious Bronchitis Virus, Ekasingh Sarueng, Wisanu Wanasawaeng, Jiroj Sasipreeyajan, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The aim of this study was to evaluate the efficacy of live infectious bronchitis (IB) vaccine programs against infection by a QX-like strain of the IB virus isolated in Thailand. The experimental groups were divided into 5 groups. Group 1 broilers were administered with Poulvac IB QX-like at 1 day old. Group 2 broilers were administered with Poulvac IB QX-like at 1 and 14 days old. Group 3 broilers were administered with Nobilis IB H120 and Poulvac IB QX-like at 1 and 14 days old, respectively. Group 4 broilers were administered with Nobilis IB H120 and Nobilis IB 4-91 at …
The Effectiveness Of Vaccination With Two Live Infectious Bronchitis Vaccine Strains Against Qx-Like Infectious Bronchitis Virus Isolated In Thailand, Tawatchai Pohuang, Niwat Chansiripornchai, Achara Tawatsin, Jiroj Sasipreeyajan
The Effectiveness Of Vaccination With Two Live Infectious Bronchitis Vaccine Strains Against Qx-Like Infectious Bronchitis Virus Isolated In Thailand, Tawatchai Pohuang, Niwat Chansiripornchai, Achara Tawatsin, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
The purpose of this study was to evaluate the level of protection generated by vaccination with 2 live attenuated vaccine strains against QX-like infectious bronchitis virus (IBV) isolated in Thailand. Eighty-four 1-day-old broiler chickens were randomly allocated into 6 groups with 14 chickens in each group. Groups 1-4 were vaccinated with different vaccination programs against IBV. Groups 5 and 6 did not receive any IBV vaccine and served as positive and negative control groups, respectively. At 1 day old, the chickens in groups 1 and 4 were vaccinated with H120 vaccine whereas the chickens in groups 2 and 3 were …
Study On The Relationship Between Single Nucleotide Polymorphisms Of The Coccidiosis-Resistance Candidate Zyxin Gene Exon 1 And Carcass Traits In The Jinghai Yellow Chicken, Guojun Dai, Dahui Sun, Mingming Sun, Yuxin Lin, Xiang Wang, Genxi Zhang, Kaizhou Xie, Miaomiao Shi, Olajide Olowofeso, Jinyu Wang
Study On The Relationship Between Single Nucleotide Polymorphisms Of The Coccidiosis-Resistance Candidate Zyxin Gene Exon 1 And Carcass Traits In The Jinghai Yellow Chicken, Guojun Dai, Dahui Sun, Mingming Sun, Yuxin Lin, Xiang Wang, Genxi Zhang, Kaizhou Xie, Miaomiao Shi, Olajide Olowofeso, Jinyu Wang
Turkish Journal of Veterinary & Animal Sciences
Previous research revealed that several single nucleotide polymorphisms (SNPs) of zyxin were significantly associated with coccidiosis-resistance parameters. In this research, the Jinghai yellow chicken was chosen to detect the SNPs of zyxin gene exon 1 by PCR-SSCP and DNA sequencing techniques, and was also used to analyze the effect of zyxin SNPs on carcass traits. A single mutation was found with 3 genotypes, designated as AA, AB, and BB. The results showed that BB was significantly different from AA and AB in terms of abdominal fat weight and slaughter rate for cockerel (P < 0.05), while there was no significant difference between AA and AB (P > 0.05). The heart weight, breast muscle rate, …
Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, Jassada Ruttanavut, Koh En Yamauchi
Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, Jassada Ruttanavut, Koh En Yamauchi
Turkish Journal of Veterinary & Animal Sciences
To evaluate the effects of a recycled food waste (eco-feed) containing effective microorganisms derived from Japanese mugwort silage juice (EJP) on growth performance and intestinal histomorphology, 48 male chickens of 14 days old were divided into 0%, 20%, 40%, and 60% EJP diet groups. At 20 weeks of age, body weight gain was similar in the 20% EJP group but lower in the 40% and 60% EJP groups (P < 0.05) compared to that in the control group. The relative total intestinal length and weight tended to be long and heavy, respectively, in the 20% and 40% EJP groups, but short and light, respectively, in the 60% EJP group. Although numerous villi were found in the jejunum and ileum of all EJP groups, the villus height and size observed in these groups were smaller. Cell area and cell mitosis number in the duodenum and jejunum of all EJP groups were lower than those of the control group (P < 0.01). Epithelial cells on the villus apical surface of the 20% EJP group showed morphology similar to that of cells on the villus apical surface of the control group in all of the intestinal segments. The growth performance data suggested that EJP could be incorporated at up to a level of 20% in chicken diets. The intestinal histological results suggested that long-term feeding of an EJP diet at up to the 40% level may result in a long and heavy intestine by increasing the numbers of miniature intestinal villi.
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen
The Thai Journal of Veterinary Medicine
Microarray containing 13319 probes was used to construct gene expression profiles in order to screendifferentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism relatedwith muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, ofwhich 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism,fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile,some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they werepresumed to be unknown new genes. The differential expressions of …
Cloning And Analysis Of Pirnas From Testes Of Three Species, Guobin Chang, Rong Chen, Ying Zhang, Aiqin Dai, Teng Ma, Guohong Chen
Cloning And Analysis Of Pirnas From Testes Of Three Species, Guobin Chang, Rong Chen, Ying Zhang, Aiqin Dai, Teng Ma, Guohong Chen
The Thai Journal of Veterinary Medicine
piRNAs are a testis-specific class of small RNAs in mammal. Sixteen sequences of piRNAs were cloned fromthree different species by the method of construction of cDNA library including two sequences from mouse, eightsequences from quail and six sequences from chicken. Herein, the two piRNAs sequences from mouse had alreadybeen identified, while the others were cloned for the first time. The sequence analysis indicated that the avian piRNAshad a similar length and a preference of uridine for 5’-end with varying degrees to the mammalian piRNAs. Besidethe function in the process of sperm development and maturation, avian piRNAs may also have a …
Phenotypic Characterisation Of Enterococcus Spp. From Femoral Head Necrosis Lesions Of Chickens, Nevi̇n Oktay, Seran Temelli̇, K. Tayfun Çarli
Phenotypic Characterisation Of Enterococcus Spp. From Femoral Head Necrosis Lesions Of Chickens, Nevi̇n Oktay, Seran Temelli̇, K. Tayfun Çarli
Turkish Journal of Veterinary & Animal Sciences
Two types of Enterococcus species were isolated from femoral head necrosis lesions of chickens. A total of 150 femoral articular tissues, of which 121 were from commercial broilers, 18 from broiler breeders, and 11 from layer breeders, were sampled. Out of 48 Enterococcus isolates of these clinical samples, 37 and 11 isolates were identified as Enterococcus faecalis and Enterococcus faecium, respectively. Additionally, 42 (28%) Escherichia coli and 33 (22%) Staphylococcus aureus isolates were obtained either alone or together with 1 of the 2 Enterococcus species from the same clinical samples, while no bacteria were detected from the remaining 56 samples. …
Single Strand Conformation Polymorphism In Intron I Of The Chicken Apovldl-Ii Gene, And Its Relationship With Triglyceride And Very Low Density Lipoprotein Concentrations, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Wen B. Bao, Adam H. Suleiman
Single Strand Conformation Polymorphism In Intron I Of The Chicken Apovldl-Ii Gene, And Its Relationship With Triglyceride And Very Low Density Lipoprotein Concentrations, Hassan Hussein Musa, Guo H. Chen, Jin H. Cheng, Wen B. Bao, Adam H. Suleiman
Turkish Journal of Veterinary & Animal Sciences
Genetically fat and lean chickens reared identically in the same environment were used to investigate the effect of the apoVLDL-II gene (intron I) on total cholesterol, triglyceride, and lipoprotein concentrations. The overall allele frequency in the 2 populations was 0.59 for allele A and 0.41 for allele B. The allele frequency in the Rugao breed was 0.52 for allele A and 0.48 for allele B; however, in the Anka breed it was 0.65 for allele A and 0.35 for allele B. The apoVLDL-II genotype in the genetically fat Anka breed was associated with a significant increase in serum triacylglyceride concentration …
Pathological And Biochemical Effects Of Intramuscular Gentamicin Administration In Chickens, Ijaz Khan, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Ijaz Javed, Ahrar Khan
Pathological And Biochemical Effects Of Intramuscular Gentamicin Administration In Chickens, Ijaz Khan, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Ijaz Javed, Ahrar Khan
Turkish Journal of Veterinary & Animal Sciences
The objective of this experimental study was to examine the pathological effects of the intramuscular administration of gentamicin to growing chickens. Two experiments of 35 days´ duration were conducted simultaneously. In both experiments White Leghorn cockerels of 6 weeks of age were randomly divided into 4 groups and gentamicin was administered intramuscularly to the birds on days 1 and 3 at a dose of 10, 50, 100, and 0 mg/kg body weight, respectively. Birds administered 50 and 100 mg/kg gentamicin showed depression, diarrhea, increased water intake, and lower body weights. Mortality was 0%, 26%, and 100% in the 10, 50, …