Veterinary Medicine Commons^™

Choledochoduodenostomy For The Treatment Of Extrahepatic Bile Duct Carcinoma In A Cat, Qi Zhang, Chunli Han, Anchi Zhang, Zhiying Wan, Yansong Ge, Zheng Wang, Jiasan Zheng

The Thai Journal of Veterinary Medicine

This paper reports a case of feline cholangiocarcinoma, a relatively rare condition in veterinary clinical practice. A 4-year-old female British Shorthair cat was presented with a 10-day history of anorexia and lethargy. An irregularly shaped mass was palpable on abdominal palpation. Diagnostic imaging and serum biochemical findings indicated extrahepatic biliary obstruction, and the diagnosis of cholangiocarcinoma was confirmed by histopathology and immunohistochemistry. Preoperative imaging in this cat indicated that the mass had not invaded major blood vessels such as the portal vein, and no evidence of distant metastasis was detected. The mass was surgically resected, followed by choledochoduodenostomy. The cat …

Synchronous Tri-Cavitary Biphasic Malignant Mesothelioma In A Dog Presenting With Dyspnea, Nawarus Prapaiwan, Tawewan Issarankura Na Ayudhaya, Poonavit Pichayapaiboon, Onrampha Tanglakmankhong

The Thai Journal of Veterinary Medicine

Malignant mesothelioma is an uncommon tumor that has a challenging diagnosis and a tendency for late diagnosis. A 7-year-old intact male dog presented with respiratory distress. Thoracic radiography revealed pleural effusion and bone reactions at multiple ribs. A computerized tomography scan with rib biopsy was suggested as a diagnosis plan. Unfortunately, the dog died. Gross pathological findings demonstrated multifocal to coalescing, pinkish-white firm masses scattered over the parietal pleural surface, extended to the serosa of the diaphragm and the pericardium, making them adhere to each other and causing the coalescence of a white irregular mass at the diaphragm. Multiple white …

Fatal Disseminated Toxoplasmosis In Captive Red Kangaroo (Macropus Rufus) And Parma Wallaby (Macropus Parma): Pathological And Immunohistochemical Investigations, Sawang Kesdangsakonwut

The Thai Journal of Veterinary Medicine

Abstract

Toxoplasmosis is a protozoan zoonosis caused by Toxoplasma gondii. Marsupials are considered a susceptible intermediate host. T. gondii is mainly transmitted to intermediate hosts via the fecal-oral route. A captive red kangaroo was diagnosed with disseminated toxoplasmosis based on clinicopathological and immunohistochemical (IHC) investigations. Two years later, a five-year-old Parma wallaby was kept in the same area as the red kangaroo and showed clinical signs of depression, withdrawal from the troop, and weakness. The wallaby died within two days after showing the clinical signs. Pathological findings in this wallaby were compatible with disseminated toxoplasmosis. Tachyzoites and tissue cysts …

Lingual Histiocytic Sarcoma In A Dog: A Case Report, Seulgi Bae

The Thai Journal of Veterinary Medicine

An 8-year-old spayed Maltese dog was presented for the evaluation of a lingual mass. The dog had excessive drooling and dysphagia. Oral examination revealed a soft, fluctuating mass at the left side of the tongue. Computed tomography showed a 21 × 32 × 66 mm lingual mass and enlarged left medial retropharyngeal lymph node. On cytology of the lingual mass, a round cell tumor was suspected; however, it was not responsive to glucocorticoid therapy. Because of severe complications, glossectomy and lymphadenectomy were performed; unfortunately, the patient died because of dyspnea in the postoperative period. Histopathologic examination and immunohistochemistry revealed lingual …

Virological And Pathological Investigation Of Canine Parvovirus-2 (Cpv-2) With The Assessment Of The Genetic Variability Of Field Strains, Zeynep Karapinar, Musa Karaman, İhsan Kisadere, Mustafa Usta, Mehmet Özkan Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

Canine parvovirus-2 (CPV-2), which is common worldwide, is a highly contagious pathogen in domestic dogs and some wild carnivore species. The agent causes a multisystemic infection in affected animals, especially acute hemorrhagic enteritis and fatal myocarditis in puppies. The study aimed to reveal the presence of virus in dogs with clinical symptoms of CPV-2 infection using virological, pathological, and immunohistochemical methods and make molecular characterization based on partial VP-2 gene of strains circulating in the region. As a result of PCR studies, 9 out of 16 rectal swab samples and 17 out of 38 blood samples were positive. A phylogenetic …

Distribution Of The Misfolded Isoform Of The Prion Protein In Peripheral Tissues And Spinal Cord Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Naturally Occurring Chronic Wasting Disease, Terry R. Spraker, Thomas Gidlewski, J. G. Powers, T. A. Nichols, M. A. Wild

United States Department of Agriculture Wildlife Services: Staff Publications

Chronic wasting disease (CWD) is an infectious transmissible spongiform encephalopathy of cervids associated with the presence of a misfolded prion protein (PrPCWD). Progression of PrPCWD distribution has been described using immunohistochemistry and histologic changes in a single section of brain stem at the level of the obex resulting in scores from 0 (early) to 10 (terminal) in elk with naturally occurring CWD. Here we describe the spread and distribution of PrPCWD in peripheral tissues and spinal cord in 16 wild and 17 farmed Rocky Mountain elk (Cervus elaphus nelsoni) with naturally occurring CWD and correlate these findings with obex scores. …

Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …

Expression Of Prostaglandin F2 Alpha Receptors (Ptgfr) And C-Reactive Protein (Crp) In The Canine Uterus And Cervix With Opened- And Closed-Cervix Pyometra, Narong Tiptanavattana, Pokchon Khirilak, Theerawat Tharasanit, Natapol Duangtum, Kijsopon Jankaew, Prawit Sae-Jia, Nongnapat Kaewchana

The Thai Journal of Veterinary Medicine

Pyometra is a common reproductive disorder in female dogs. This study investigated the protein expression of prostaglandin F2 alpha receptors (PTGFR) and C-reactive protein (CRP) in responses to inflammatory events of uterine and cervical tissues in dogs. Uterus and cervix samples were collected from six dogs which had closed-cervix pyometra (n = 3) and opened- cervix pyometra (n = 3). Healthy dogs in diestrus (n = 3) and anestrus (n=3) were used as controls. Tissues were histologically prepared and stained with hematoxylin and eosin. Immunohistochemistry (IHC) and western blot analysis for the PTGFR and CRP were used to verify the …

Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk

The Thai Journal of Veterinary Medicine

In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace  Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …

Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici

The Thai Journal of Veterinary Medicine

In this study, the possible protective effect of red radish containing total phenolic content and antioxidant components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity, triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated in CCl4induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw); while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was …

Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile

The Thai Journal of Veterinary Medicine

A mature intracranial teratoma was identified in a 5-month-old pet rabbit showing a sudden onset of obtundation, recumbency, flaccid tetraplegia, and hyporeflexia. A space-occupying suprasellar, irregular cystic mass extending from the thalamus to the brainstem was observed at necropsy. Histologically, the tumor was composed of irregular, multilocular cysts lined by a thin wall and interposed tissue where a heterogeneous collection of structures including adipose and myxomatous tissues, cartilage, glandularand ductal structures, skeletal muscle, nervous tissue, nerve fascicles, sebaceous glands, and exocrine pancreatic cells were recognized. Immunohistochemistry showed labeling of the diverse tissues with cytokeratin, vimentin, smooth muscle actin, GFAP, NSE, …

A Nasopharyngeal Inflammatory Polyp In A Cat: Histopathology, Immunohistochemistry, And Ct Scan Findings, Hamid Reza Moosavian, Hamideh Esmaeilzadeh, Ramesh Vahabi, Rana Vafaei, Mohammad Reza Esmailinejad

Turkish Journal of Veterinary & Animal Sciences

The etiology of feline nasopharyngeal inflammatory polyps (FNIPs) has not been completely understood. To the best of our knowledge, there is no study having evaluated the immunohistochemical features of FNIPs. More studies about the histopathological characteristics of such lesions can play a pivotal role in further understanding the disease. We present the clinical manifestations, computed tomography (CT) scan, histopathology, and immunohistochemistry features of a FNIP in a 7-year-old male neutered domestic shorthair cat. CT scan examination showed nasopharyngeal space occupation with some parts of destruction in nasal cavity. Immunohistochemical study of the sections demonstrated the presence of CD3$^{+}$ lymphocytes, and …

Ovarian Stimulation Induces High Expression Of Interleukin-1ss And Disrupts The Histological Features Of The Fallopian Tube, Mahmoud Moussa, Sameh Mohamed Farouk

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate whether there is an association between ovarian stimulation and interrupted gamete interaction in the rat fallopian tube in vivo. We evaluated the protein expression of interleukin-1ß (IL-1ß) via immunohistochemistry and assessed the key histological features of the ovaries, fallopian tubes, and uteri of the rats following treatment with mild (15 IU) and high (30 IU) doses of gonadotropins compared to an unstimulated control group. The serum concentration of estradiol was also determined by immunoassay. The results revealed that high levels of circulating estrogen were associated with higher expression of the IL-1ß protein …

Immunohistochemical Expression Of Secretory Proteins In Pneumonic Rat Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study evaluated the expression of secretory proteins involving surfactant proteins (SP-A, SP-B, proSP-C), Clara cell secretory protein (CCSP), and thyroid transcription factor-I (TTF-I), together with lambda light chain IgG (λ-IgG), proliferating cell nuclear antigen (PCNA), and lymphocytic phenotypes (T and B cells) in the pneumonic rat lungs. The most prominent gross lesion was severe pulmonary abscession. The lungs showed severe parenchymal destruction and necrosis encapsulated by fibrous tissue. Immunostaining for SPs displayed evident hyperplasia of type II pneumocytes. Immunoreaction to SP-A and SP-B occurred strongly on the luminal surface of type II cells, and to proSP-C in the …

Equine Arteritis Virus Has Specific Tropism For Stromal Cells And Cd8+ T And Cd21+ B Lymphocytes But Not For Glandular Epithelium At The Primary Site Of Persistent Infection In The Stallion Reproductive Tract, Mariano Carossino, Alan T. Loynachan, Igor F. Canisso, Richard Frank Cook, Juliana Roberta Campos, Bora Nam, Yun Young Go, Edward L. Squires, Mats H. T. Troedsson, Thomas W. Swerczek, Fabio Del Piero, Ernest F. Bailey, Peter J. Timoney, Udeni B. R. Balasuriya

Veterinary Science Faculty Publications

Equine arteritis virus (EAV) has a global impact on the equine industry as the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of equids. A distinctive feature of EAV infection is that it establishes long-term persistent infection in 10 to 70% of infected stallions (carriers). In these stallions, EAV is detectable only in the reproductive tract, and viral persistence occurs despite the presence of high serum neutralizing antibody titers. Carrier stallions constitute the natural reservoir of the virus as they continuously shed EAV in their semen. Although the accessory sex glands have been implicated as …

Study Of Testicular Immunolocalization Of Inhibin Subunits And Epididymal Histological Structures Among Different Antler Status In Rusa Deer (Rusa Timorensis), Apichaya Sudsukh, Kazuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

The number of rusa deer (Rusa timorensis) raising farms has highly increased in Thailand. Basic data on the reproduction of rusa deer are essential for improvement in deer farming reproduction and the assisted reproductive technologies (ARTs) of endangered deer species. This study aimed to determine the relation of antler changes to testicular and epididymis histological structure and cellular source of testicular inhibin subunits in rusa deer. Eighteen rusa deer testes and epididymis were collected from a slaughterhouse located in Kamphaeng Saen, Nakhon Pathom, Thailand. The male rusa deer used in this study were separated into three groups depending on antler …

Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …

Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur

Turkish Journal of Veterinary & Animal Sciences

A 10-year-old spayed female Maltese dog presented to a local animal hospital with a subcutaneous mass (4 x 3 x 3 cm) in the right shoulder region. The mass was well circumscribed, with soft tissue opacity and variable levels of mineralization, but with no bony involvement in radiography. The mass was surgically removed. Upon histological examination, the mass consisted of malignant mesenchymal cells that had produced a chondroid matrix and osteoid. Round to polygonal neoplastic cells with large nuclei showed moderate anisokaryosis and variable numbers of mitotic figures. The tumor cells were positive for vimentin and osteocalcin and negative for …

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …

Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong

The Thai Journal of Veterinary Medicine

Our previous study reported the expression of kisspeptin in the preoptic area (POA) and arcuate nucleus (ARC) of the hypothalamus of cycling buffalo cows. In ruminants, POA and ARC are the main hypothalamic nuclei through which kisspeptin acts on the hypothalamic-pituitary-gonadal axis, due to their stimulation of gonadotropin releasing hormone (GnRH) secretion. In many species, kisspeptin activates GnRH neurons via its receptors (KISS1R or GPR54). The present study was designed to explore the expression of KISS1R by GnRH neurons in the POA and ARC hypothalamic nuclei in buffalo cow brains by double-labeling immunohistochemistry. In both the POA and ARC areas, …

Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …

Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.

The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes

Open Access Dissertations

Approximately 7.5 million Americans are affected by a voice disorder. In the last year, 7.2% of people missed one or more days of work due to a voice problem. For professional voice users, such as teachers, the rate increases to 20% and an annual cost of $2.5 billion. Voice disorders are complex and multi-faceted, as well as difficult to diagnose and treat. Prospective animal studies are necessary to study the pathophysiology of voice disorders, optimize our understanding of laryngeal pathology, and improve treatment outcomes. The pig provides a unique opportunity to test hypotheses relating to laryngeal disease because porcine vocal …

Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem

Turkish Journal of Veterinary & Animal Sciences

In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …

Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha

Turkish Journal of Veterinary & Animal Sciences

Merkel cell tumors, a type of cutaneous round-cell tumors, are rarely observed in dogs and cats. This tumor type originates from cutaneous neuroendocrine cells. In the present study, the morphology of canine and feline Merkel cell tumors and their immunoexpression of neuron-specific enolase, cytokeratin, chromogranin A, Ki67, and metallothionein were evaluated using histopathological and immunohistochemical methods. The canine Merkel cell tumor displayed a diffuse morphological pattern, whereas the feline tumor displayed a solid morphological pattern. Both tumors were positive for neuron-specific enolase and cytokeratin, but negative for chromogranin A. The Ki67 indexes in the canine and feline tumors were 7% …

Histopathological And Immunohistochemical Characterization Of Spontaneous Uterine Leiomyomas In Two Captive Asian Elephants, Kidsadagon Pringproa, Hiroo Madarame, Jiraporn Sritun, Pitikarn Bumpenpol, Paninee Pedsri, Chalermchart Somgird, Chatchote Thitaram

The Thai Journal of Veterinary Medicine

Two captive female Asian elephants (Elephas maximus) in Thailand were euthanatized after prolonged recumbency, weakness, and unresponsive treatment. Necropsy revealed multinodular masses of 5-10 cm in diameter which were irregular, large, white and firm in consistency scattered throughout the uterine horns and uteri of both the elephants. Histologically, the tumors displayed mildly pleomorphic, spindle-shaped cells arranged in interlacing pattern interpreted as muscle bundles. The bundles of the muscle cells were interspersed by small amounts of collagen fibers and blood vessels. For immunohistochemistry, the tumor cells showed diffuse positivity for vimentin, desmin, and smooth muscle actin. Based on the pathological results, …

Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar

The Thai Journal of Veterinary Medicine

Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the …

The Effects Of Vincristine Sulfate On Expression Of Galectin-3, Bcl-2, And Carbohydrate Structures Specific For Eel, Gsl-1, And Rca-1 Lectins In Bitches With Naturally Occurring Canine Transmissible Venereal Tumor, Musa Özgür Özyi̇ği̇t, Deni̇z Nak, Hasan Akşi̇t, Sevda İnan Öztürkoğlu, Gözde Şi̇mşek, Ender Uzabaci, Yavuz Nak, Kami̇l Seyrek

Turkish Journal of Veterinary & Animal Sciences

Vincristine sulfate is one of the most effective chemical agents used in canine transmissible venereal tumor (CTVT) chemotherapy. Its therapeutic effectiveness is pronounced and significant at the beginning of treatment. The present study was designed to investigate vincristine sulfate treatment and the relationship between CTVT tumor regression and apoptosis [estimated by TdT-mediated dUTP-biotin nick end labeling (TUNEL)], galectin-3 (H-160), Bcl-2 (N-9), and lectin [EEL (B-135), GSL I (L-1100), and RCA I (BA-0084)] expression using immunohistochemical staining in 20 bitches with naturally occurring CTVTs. Evaluation was semiquantitatively performed by HSCORE values using light microscopy. All observed staining was intracytoplasmic and increased …

Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study is to investigate the effect of exogenous administration of melatonin, which is a strong antioxidant agent, on the gene expression of glutathione peroxidase, which is one of the antioxidant enzymes, in the livers of mice and also to examine the immunohistochemical localization of the enzyme. The 24 male Swiss albino mice used in the study were divided into 3 groups: experimental (n = 8), sham (n = 8), and control (n = 8). A 10 mg/kg dose of melatonin was administered intraperitoneally to the experimental group for 4 weeks. Only ethanol and a serum physiological …

Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük

Turkish Journal of Veterinary & Animal Sciences

The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the …