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Full-Text Articles in Veterinary Medicine
Immunoinformatics-Based Design And In Vitro Expression Analysis Of A Multi-Epitope Vaccine Against African Swine Fever Virus, Dhithya Venkateswaran
Immunoinformatics-Based Design And In Vitro Expression Analysis Of A Multi-Epitope Vaccine Against African Swine Fever Virus, Dhithya Venkateswaran
Chulalongkorn University Theses and Dissertations (Chula ETD)
African swine fever (ASF) is a lethal haemorrhagic disease affecting domestic and wild pigs, causing severe economic losses globally. The absence of an effective vaccine has hindered ASF control, as traditional approaches are challenged by the virus’s genetic complexity and immune evasion mechanisms. In this study, we employed immunoinformatics to design a multi-epitope vaccine (MEV) against African Swine Fever Virus (ASFV), targeting conserved viral proteins—B646L (p72), CP204L (p30), E183L (p54), and EP402R (CD2v). Antigenic and non-allergenic BL and HL epitopes were selected and assembled with appropriate adjuvant and linkers to construct the MEV. No CTL epitopes were included, as they …
Canine Uterine Fibrosis Associated With Age And Pyometra, Maulida Selma Hanim
Canine Uterine Fibrosis Associated With Age And Pyometra, Maulida Selma Hanim
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study investigated whether aging and uterine pathology drive fibrosis in the canine uterus by quantifying collagen deposition in endometrial and myometrial compartments. Uterine samples from 55 small-sized, intact, nulliparous bitches were classified into five groups according to age and pathological conditions. Healthy group divided into juvenile, young adult, old adult, and the pathological group included cystic endometrial hyperplasia (CEH), and pyometra. Fibrosis was assessed using Masson’s trichrome staining; collagen proportion was quantified with ImageJ and analysed in SAS. Across healthy age groups, collagen proportions in the endometrial stroma, circular myometrium, and longitudinal myometrium did not differ significantly (P > 0.05). …
Expression Of Peroxisome Proliferator-Activated Receptor Alpha In Feline Mammary Carcinomas, Ngoc Anh Van Pham
Expression Of Peroxisome Proliferator-Activated Receptor Alpha In Feline Mammary Carcinomas, Ngoc Anh Van Pham
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate the expression patterns of peroxisome proliferator-activated receptor alpha (PPARα) in normal tissue and feline mammary carcinomas. Thirty feline mammary carcinomas (FMCs) and eight normal mammary tissues were used in this study. In the same FMC cats, tissues were collected from 2 regions which are the neoplastic tissue and the adjacent non-neoplastic tissues. The study was composed of three parts: (1) clinical data collection and histopathological diagnosis; (2) Immunohistochemistry (IHC) evaluation of PPARα, Ki-67, and proliferating cell nuclear antigen (PCNA); and (3) Western blot analysis of PPARα isoforms. The results revealed no significant differences in PPARα …
Diagnostic Approaches And Therapeutic Evaluation In Canine Benign Prostatic Hyperplasia: An Integrated Analysis Of Prostatic Volume, Serum Canine Prostatic Specific Esterase (Cpse) Levels, And Proteomic Profiles, Panthipa Borikappakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Benign prostatic hyperplasia (BPH) is a highly prevalent condition in intact male dogs. However, its diagnosis and clinical management remain limited by the lack of clear thresholds for clinically significant prostatic enlargement, insufficient biomarkers to monitor treatment response, and an incomplete understanding of the molecular mechanisms driving disease progression. To address these gaps, this thesis integrated ultrasonographic, biochemical, and proteomic approaches across three complementary studies. First, the actual to expected normal prostatic volume ratio was evaluated to establish a clinically meaningful cutoff distinguishing subclinical from clinical BPH, providing an imaging-based indicator for early recognition of clinically relevant enlargement. Building on …
Community Ecology Of Domestic Animals And Wildlife At Solid Waste Disposal Sites Near Dong Phayayen – Khaoyai Forest Complex And Its Impacts On Zoonotic Diseases, Paisin Lekcharoen
Community Ecology Of Domestic Animals And Wildlife At Solid Waste Disposal Sites Near Dong Phayayen – Khaoyai Forest Complex And Its Impacts On Zoonotic Diseases, Paisin Lekcharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Solid waste disposal sites (SWS) at forest edges represent critical zoonotic transmission hotspots where wildlife, domestic animals, and humans converge. This study characterized host community structure, pathogen circulation, and network topology at SWS adjacent to the Dong Phayayen-Khao Yai Forest Complex, Thailand. Three study phases (2021-2023) comprised: (1) camera trapping at five SWS (3,907 trap-nights); (2) small mammal trapping across four habitats (1,420 individuals, 12 species), ectoparasite collection (20,560 chiggers, 14 species), and molecular pathogen detection; and (3) network analysis of connectivity among habitat, host, chigger, and pathogen. Domestic dogs dominated communities (88.3%), creating simplified assemblages. Potential transmission operated through …
Optimization Of Artificial Insemination In Swine Through Gnrh Agonist Application In Boar Semen With Special Emphasis On Epithelial Penetration And Pharmacokinetics, Preechaphon Taechamaeteekul
Optimization Of Artificial Insemination In Swine Through Gnrh Agonist Application In Boar Semen With Special Emphasis On Epithelial Penetration And Pharmacokinetics, Preechaphon Taechamaeteekul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Induction of ovulation in pigs is a widely applied technique in commercial swine farms, using hormonal treatments to improve reproductive efficiency and productivity. The GnRH agonist buserelin acetate is widely used to induce ovulation and can be administered via intramuscular or intravaginal routes. However, the success of ovulation induction via the intravaginal route still depends on several factors, and information regarding the pharmacokinetics and tissue permeation of buserelin remains limited. This thesis aimed to investigate the pharmacokinetic profile, endometrial permeability, and reproductive effects of buserelin to develop an effective and practical ovulation control strategy for commercial swine production. This thesis …
Surveillance And Genetic Characterization Of Atypical Porcine Pestivirus (Appv) In Central Thailand, Sana Shahid
Surveillance And Genetic Characterization Of Atypical Porcine Pestivirus (Appv) In Central Thailand, Sana Shahid
Chulalongkorn University Theses and Dissertations (Chula ETD)
Atypical Porcine Pestivirus (APPV) is an emerging swine pathogen associated with congenital tremor (CT) Type A-II, a neurological condition characterized by tremors of the head, body, and limbs, as well as poor growth in piglets. From September 2023 to August 2025, nasal and blood samples were collected from 181 pigs from four commercial pig farms in Central Thailand, and APPV was detected in 17 samples. Notably, all APPV-positive samples come from one farm. A follow-up study was conducted at the infected farm to assess APPV persistence and its association with age groups and clinical signs. In total, 275 pigs were …
Genomic Characterization Of Streptococcus Agalactiae And Streptococcus Suis Isolated From Diseased Snakeskin Gourami (Trichopodus Pectoralis), Surawit Chueahiran
Genomic Characterization Of Streptococcus Agalactiae And Streptococcus Suis Isolated From Diseased Snakeskin Gourami (Trichopodus Pectoralis), Surawit Chueahiran
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study investigates severe, recurrent mortality outbreaks in farmed snakeskin gourami (Trichopodus pectoralis) in central Thailand between 2021 and 2023. Characterized by exophthalmos and systemic hemorrhage, these incidents prompted an investigation to identify causative agents, assess antimicrobial resistance (AMR), and explore genomic adaptation. Bacterial isolates from moribund fish in Samut Sakhon province were identified via MALDI-TOF MS and 16S rRNA sequencing, followed by whole-genome sequencing (Illumina HiSeq) and comparative pan-genomic analysis. Two primary pathogens, Streptococcus agalactiae and Streptococcus suis, were confirmed as causative agents of acute bacterial septicemia, occurring in both single and co-infections. Phylogenetic analysis revealed that S. suis …
Expression Patterns Of Alpha-Fetoprotein And Phosphodiesterase 4d In Canine Mammary Tumors, Thi Kieu Trinh Mai
Expression Patterns Of Alpha-Fetoprotein And Phosphodiesterase 4d In Canine Mammary Tumors, Thi Kieu Trinh Mai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine mammary tumors (CMTs) represent one of the most common neoplasms in female dogs, with a substantial proportion exhibiting malignant behavior and aggressive progression. Despite their clinical importance and relevance to comparative oncology, the molecular mechanisms underlying CMT development remain incompletely understood. This study aimed to evaluate the expression patterns of alpha-fetoprotein (AFP) and phosphodiesterase 4D (PDE4D) in canine mammary tissues, and to investigate their correlations with Ki-67, a standard marker of cell proliferation. A total of 50 dogs were included in this study (40 diagnosed with CMTs and 10 without CMTs), yielding 90 FFPE tissue samples for protein analysis …
Distribution Pattern And Risk Factors Of Foot And Mouth Disease Outbreaks In Java Island, Indonesia, Virgilius Martin Kelake Kedang
Distribution Pattern And Risk Factors Of Foot And Mouth Disease Outbreaks In Java Island, Indonesia, Virgilius Martin Kelake Kedang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Indonesia experienced new outbreaks of foot and mouth disease (FMD) in 2022 after being officially free from the disease for several decades. The outbreak started in April 2022 in East Java and rapidly spread to many regions in Indonesia. This study aimed to investigate the distribution pattern and risk factors associated with the FMD outbreaks in Java Island, Indonesia, from 2022 to 2023. Descriptive, spatial, spatiotemporal, and risk factor analyses were employed to examine the outbreak dynamics and contributing factors. The results indicated that the outbreaks were distributed across the island, with East Java being the most affected region. The …
Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul
Epidemiological And Molecular Studies On Lumpy Skin Disease Outbreaks In Beef Cattle In Thailand During 2021-2022, Phurida Sripipattanakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lumpy skin disease (LSD) is a vector-borne viral disease of cattle caused by the Lumpy skin disease virus (LSDV). LSD is considered as an emerging infectious disease in cattle in Asia and poses a significant threat to cattle production across Africa, Europe, and Asia. This study aimed to describe the epidemiological characteristics of LSD outbreaks in beef cattle in Thailand and to conduct a molecular survey of LSDV infection in beef cattle from May 2021 to December 2022. The study included 594 beef cattle from 257 farms affected by the LSD outbreaks in 28 provinces. We collected hair samples (n=492) …
Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs, Apichart Prapattanakullawong
Evaluating The Efficacy Of An Early Vaccination Strategy After Farrowing Using A Modified Live Prrs Vaccine And Post-Weaning Challenge With Thai Hp-Prrs, Apichart Prapattanakullawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen responsible for substantial economic losses in the global swine industry, adversely affecting both reproductive and respiratory health. In Thailand, early PRRSV infections in suckling and nursery pigs are commonly observed, necessitating the implementation of early vaccination strategies. However, early vaccination in young piglets has been controversial due to concerns about interfering maternal-derived antibodies (MDA) and the competency of the piglets' immune system. The objective of this study is to evaluate the efficacy of a modified-live virus (MLV) PRRS vaccination in early-age piglets from PRRSV-positive farms by assessing immune responses …
Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok, Thawanrut Kiatyingangsulee
Genotypic Characterization And Amr Risk Assessment Of Esbl- Producing And Fluoroquinolone-Resistant Salmonella Spp. From Pork Collected In Bangkok, Thawanrut Kiatyingangsulee
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to 1) determine the prevalence of ESBL-producing and fluoroquinolone-resistant Salmonella in pork, 2) investigate their genomic characteristics, and 3) estimate the risk of consumer exposure. Pork samples were collected from 58 markets across 19 districts in Bangkok. The study focused on third-generation cephalosporin resistance mediated by ESBLs and fluoroquinolone resistance, representing horizontal and vertical gene transfer, respectively. Out of 793 pork samples, 339 (42.8%) were positive for Salmonella, yielding 531 isolates from 33 serotypes. The most common were Salmonella Rissen (22.6%), Sinstorf (16.8%), and Derby (10.2%). Antimicrobial susceptibility testing showed 28.2% of isolates were ESBL producers and …
Efficacy Of Cell-Free Supernatants Based Nano-Application From Thai Probiotic Bacteria As An Alternative Preservative In Meat Against Foodborne Pathogens, Thin Zar Soe
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study evaluates whether the use of cell-free supernatants (CFS) derived from Thai probiotic bacteria as natural preservatives in meat products, provides a safer alternative to chemical preservatives. The objectives include developing a nano-encapsulation technique to enhance the stability and efficacy of CFS, and assessing the effectiveness of nano-encapsulated CFS (Nano-CFS) in maintaining meat safety and quality during storage and evaluating the antibacterial efficacy of CFS and (Nano-CFS) against common foodborne pathogens, determining the minimal inhibitory and bactericidal concentrations (MIC and MBC). The CFS was prepared from the Thai probiotic strains. Antibacterial activity was determined using agar well diffusion and …
Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle, Wannapol Buthasane
Genomics Approach For Ex Situ Conservation Of Asian King Vulture And Steppe Eagle, Wannapol Buthasane
Chulalongkorn University Theses and Dissertations (Chula ETD)
The Asian king vulture (Sarcogyps calvus, AKV), an ecologically important forest scavenger, is an Old World vulture in the family Accipitridae. It is classified as a critically endangered species, with no genomic data currently available. This dissertation aimed to assemble a high-quality reference genome to enhance understanding of its genetic background, scavenging adaptations and genetic diversity within Thailand’s captive population. A hybrid genome assembly combining Pacific Biosciences (PacBio) long-read and MGI short-read sequencing was generated from DNA extracted from the whole blood of a female individual. Comparative genomic analyses with New World vultures (NWVs) and other Accipitridae species revealed distinct …
The Comprehensive Study Of The Reproductive Cycle With A Special Focus On The Vestibular Microbiome In Captive Asian Elephants (Elephas Maximus), Yuqing Yang
Chulalongkorn University Theses and Dissertations (Chula ETD)
This dissertation aimed to advance the understanding of reproductive physiology and health in Asian elephants, providing critical insights and innovative methodologies for reproductive management. Chapter II established a foundational framework for normal reproductive cycling, detailing the luteal and follicular phases, and introduced a simplified, practical body condition score (BCS) method as a diagnostic tool for assessing general health and welfare. It highlighted unresolved reproductive issues, such as abnormal cycling, and revealed that progesterone peaks, with the majority (53.57%), and the onset of the follicular phase predominantly occurred during the rainy season. Monitoring ovulation and maintaining a standard BCS during the …
Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination, Myzatul Hanis Zahiyah Yusof
Improving Antimicrobial Susceptibility Testing Of Arcobacter Butzleri: Comparative Methods And Epidemiological Cut-Off Value (Ecoff) Determination, Myzatul Hanis Zahiyah Yusof
Chulalongkorn University Theses and Dissertations (Chula ETD)
Arcobacter butzleri is an emerging zoonotic pathogen associated with gastrointestinal illnesses in humans, yet standardized antimicrobial susceptibility testing (AST) methods and interpretive breakpoints for this microorganism remain unavailable. Broth microdilution, despite being endorsed by the CLSI-VAST subcommittee, is often impractical in several laboratories. Therefore, this study aimed to evaluate agar dilution as a more feasible alternative to broth microdilution. A total of 415 A. butzleri isolates from various meat sources in Bangkok, Thailand were confirmed by PCR and tested against ciprofloxacin, erythromycin, gentamicin, and tetracycline. AST was conducted using broth microdilution under aerobic conditions for 24 hours as the reference …
Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats, Run Sakulsirajit
Ultrasonographic Renal Cortical Thickness To Abdominal Aorta Internal Diameterin Healthy Cats And Chronic Kidney Disease Cats, Run Sakulsirajit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Chronic kidney disease (CKD) is a one of the most common disorder in cats, particularly in aging populations. Early detection and accurate monitoring are crucial for effective disease management. This study aims, first, to evaluate the ultrasonographic parameters, including renal cortical thickness (RCT), medullary thickness (MT), renal length (RL), abdominal aorta diameter (Ao) and ratios, including RCT/Ao, RCT/MT, RCT/RL, MT/Ao and RL/Ao compared between clinically healthy and CKD cats. Second, to evaluate the correlations of ultrasonographic parameters with blood renal functions including blood urea nitrogen (BUN), creatinine (Cr) and symmetric dimethylarginine (SDMA) and also correlation with age, body weight (BW) …
Complete Genome Analysis Of African Swine Fever Virus Strains In Thailand, Anwesha Prakash
Complete Genome Analysis Of African Swine Fever Virus Strains In Thailand, Anwesha Prakash
Chulalongkorn University Theses and Dissertations (Chula ETD)
African Swine Fever Virus (ASFV) is a large double-stranded DNA virus responsible for the highly contagious and lethal African Swine Fever (ASF), affecting the global porcine industry. The virus has a genome length of 170-194 kb and is broadly categorized into the left variable region (LVR), the right variable region (RVR), and the central conserved region (CCR). In Thailand, ASF was officially confirmed in January 2022 and identified as genotype II, which is primarily responsible for the current Eurasian swine pandemic. To date, due to the absence of a globally accepted vaccine against ASFV, culling and quarantine remain the primary …
Canine Cricoid Cartilage And Tracheal Dimensions Observed On Computed Tomographic Image, Tanya Kasemsuwan
Canine Cricoid Cartilage And Tracheal Dimensions Observed On Computed Tomographic Image, Tanya Kasemsuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Inhalation anesthesia is commonly used for general anesthesia due to its effectiveness. During the procedure, an endotracheal tube (ET) is essential for maintaining anesthetic depth, delivering anesthetic agents, and providing sufficient oxygen to reduce the risks of morbidity and mortality. Mispositioning and inappropriate ET size can lead to complications like hypoxemia, hypercapnia, and airway trauma. Commercially available ET tubes are designed for general use and are not specifically optimized for different cephalic types, leading to the objective of this study, which was to investigate the differences in anatomical dimensions of the cricoid cartilage and trachea and ET location among brachycephalic, …
Effect Of Four Non-Slipping Floor Mat Types On The Stifle Joint Range Of Motion During Walking And Trotting In Small Breed Dogs With Medial Patellar Luxation Grade 2 And Normal Stifle Dogs, Panisara Wongsirichatchai
Effect Of Four Non-Slipping Floor Mat Types On The Stifle Joint Range Of Motion During Walking And Trotting In Small Breed Dogs With Medial Patellar Luxation Grade 2 And Normal Stifle Dogs, Panisara Wongsirichatchai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The two dimension kinematic motion analysis was an accessible tool for clinical approach for assist gait analysis and joint mobility in veterinary clinical field. Thermographic imaging also considered as an adjunctive non-invasive technique to detects changed superficial temperature from the physiological associated with inflammation or musculoskeletal conditions especially in joints, tendon, or muscle. A comparing controlled experiment investigated the effects of various ceramic flooring surfaces on gait, stifle joint mobility, and post-activity inflammatory response following activity in small breed dogs with and without grade 2 medial patellar luxation (MPL).Sixteen small breed dogs were enrolled, including normal stifle dogs (n=7) and …
Validation Of A Pseudotype-Based Serum Neutralization Test Forpeste Des Petits Ruminants Virus (Pprv) Specific Antibody In Goat Sera, Suphatcha Mahawattana
Validation Of A Pseudotype-Based Serum Neutralization Test Forpeste Des Petits Ruminants Virus (Pprv) Specific Antibody In Goat Sera, Suphatcha Mahawattana
Chulalongkorn University Theses and Dissertations (Chula ETD)
Peste des petits ruminants (PPR) is a transboundary viral disease in small ruminants, caused by a morbillivirus called peste des petits ruminants virus (PPRV). Recently, FAO and WOAH have set the goal to eradicate the disease in 2030. To maintain disease vigilance, the Department of Livestock Development conducts regular screenings for anti-PPRV antibodies using a commercially available competitive ELISA (cELISA). However, limitation from its window of uncertainty could lead to inconclusive result. In this study, a recently developed pseudotype-based neutralization test was validated against a large number of known positive and negative samples. In comparison with the cELISA, from 416 …
Proteomic Analysis Of Canine Reproductive Protein Profiles In Relation To Stage Of Oocyte Development, Larindhorn Udomthanaisit
Proteomic Analysis Of Canine Reproductive Protein Profiles In Relation To Stage Of Oocyte Development, Larindhorn Udomthanaisit
Chulalongkorn University Theses and Dissertations (Chula ETD)
The domestic dog has a unique reproductive physiology in which oocytes are ovulated at an immature stage and complete nuclear maturation within the oviduct approximately 48–72 hours after ovulation. This thesis aimed to investigate proteomic changes in both serum and oviductal secretions associated with oocyte development, using a combination of natural and hormonally induced ovulation models. To evaluate dynamic changes in serum proteins across the estrous cycle, samples from healthy bitches in four stages—anestrus, proestrus, estrus, and diestrus—were analyzed, revealing 38 significantly altered proteins. Among these, eight exhibited stage-specific expression patterns, including HES2, ADGRA3, and SWI5 in anestrus; polycystin receptors …
Alternative Approaches To Replace Antibiotics Use In Boar Semen Extenders And Their Effects On Reproductive Performance, Congbang Ngo
Alternative Approaches To Replace Antibiotics Use In Boar Semen Extenders And Their Effects On Reproductive Performance, Congbang Ngo
Chulalongkorn University Theses and Dissertations (Chula ETD)
Artificial insemination (AI) with antibiotic-free alternatives in boar semen extenders is becoming essential for promoting environmentally sustainable practices in the global swine industry. Comprising 4 parts, this thesis aimed to investigate the boar fecal and seminal microbiomes in relation to semen quality and probiotic supplementation in an antibiotic-free feeding program. In addition, the characteristics of semen doses treated with alternative methods and their effects on the reproductive performance of sows inseminated with these semen doses are examined. (i) To investigate the fecal and seminal microbiomes of boars in relation to semen quality and the effects of transitioning from antibiotics to …
Molecular Investigation And Genetic Characterization Of Hemotropic Mycoplasma Spp. And Potential Role Of Flea Vectors In Goats In Thailand, Muhammad Shair Zaman Ghauri
Molecular Investigation And Genetic Characterization Of Hemotropic Mycoplasma Spp. And Potential Role Of Flea Vectors In Goats In Thailand, Muhammad Shair Zaman Ghauri
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to investigate the identification of flea species infesting goats and to study the molecular prevalence and genetic characterization of hemotropic mycoplasmas in goats and fleas. A total of 500 blood and 79 flea samples were collected from goat farms across seven provinces in Thailand between January 2017 and October 2023. Among the 500 goats examined, 6.6% were infested with fleas, which were morphologically identified and molecularly characterized using mitochondrial (cox-I, cox-II) and nuclear (ITS1, EF-1α) markers as Ctenocephalides orientis. Additionally, 14,315 bp fragment of the C. orientis mitochondrial genome was analyzed, revealing a close evolutionary relationship with …
Selection Of Lactic Acid Bacteria Probiotics From Thai-Chick And Their Efficacy In Restoring Gut Microbiome And Salmonella Reduction In Broiler Challenged With Salmonella Typhimurium, Benjamas Khurajog
Chulalongkorn University Theses and Dissertations (Chula ETD)
Antimicrobial resistance (AMR), food safety, and sustainable poultry farming are critical global concerns driving the exploration of probiotics as alternatives to antibiotics. This study focused on the isolation, characterization, and evaluation of lactic acid bacteria (LAB) strains and the performance of two probiotics—ProCU (a blend of locally isolated LAB strains) and TOP GUT (a commercial Clostridium butyricum product)—in mitigating Salmonella infection, improving growth performance, and modulating the gut microbiota and resistome in broiler chickens. From 91 LAB isolates identified in chicken feces across Thailand, five strains (Ligilactobacillus salivarius BF12 and Pediococcus acidilactici BF9, BF14, BYF20, and BYF26) exhibited strong tolerance …
Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs, Panumas Kongpanna
Comparative Study Of The Effects Of Dietary Supplements On Reproductive And Growth Performance In Sows And Nursery Pigs, Panumas Kongpanna
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study evaluated the effects of dietary supplementation with a multi-strain probiotic containing Bacillus subtilis (5 × 107 CFU/g) and Lactobacillus plantarum (1 × 109 CFU/g) on reproductive performance in multiparous sows and growth performance in weaned piglets. In Experiment 1, 300 sows were assigned to control or probiotic diets across five parity groups (2, 3, 4, 5 and 6 to 9) using a 2 × 5 factorial design. Experiment 2 involved 120 weaned piglets randomly allocated to four dietary treatments: control, antibiotic, enzyme, or probiotic. Measurements included reproductive and litter performance, colostrum IgG, milk composition, and piglet growth, digestibility, …
Detection Of Brafv595e Gene Of Canine Urothelial Carcinoma Using Real-Time Pcr, Monticha Kitnitchee
Detection Of Brafv595e Gene Of Canine Urothelial Carcinoma Using Real-Time Pcr, Monticha Kitnitchee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine urothelial carcinoma (CaUC) is the most common malignant tumor of the urinary bladder in dogs. This cancer has aggressive behavior and it is challenge for diagnosis. Most CaUC cases are diagnosed from the obvious clinical signs as the late to advance stage. In addition, therapeutic strategies for this stage are almost non-responsive for the eradication and prevention of recurrent disease. Therefore, non-invasive early diagnosis is required for urothelial carcinomas in both human and animals including dogs, which have similar cancer biology to human. This study aimed to evaluate diagnostic accuracy by BRAFV595E detection using real-time PCR (qPCR) from various …
Monitoring And Characterization Of Colistin-Resistant And Extended-Spectrum Β-Lactamase-Producing Escherichia Coli And Salmonella Spp. In The Standard Slaughtering Processes., Imporn Saenkankam
Chulalongkorn University Theses and Dissertations (Chula ETD)
The rise of antimicrobial-resistant pathogens in the pig production chain poses significant threats to public health and food safety, particularly involving Salmonella and extended-spectrum β-lactamase-producing (ESBL-Ec), colistin-resistant ESBL-Ec. The slaughterhouse serves as a critical point for monitoring and preventing bacterial dissemination to humans. This study comprehensively examined the prevalence, antimicrobial resistance (AMR) profiles, and genomic characteristics of these pathogens across two pig slaughterhouses in Thailand—one operating under Hazard Analysis and Critical Control Points (HACCP) standards and the other certified only under Good Manufacturing Practices (GMP). A total of 570 samples from feces, carcasses, and environmental sources were analyzed. Salmonella prevalence …
Strategies Of Utilizing Sex-Sorted Semen To Improve Beef Cattle Herd Production And Using Lasia Spinosa Thw. And Zinc Supplementation To Improve Semen Quality Of Bulls And Boars With Poor Semen Profile, Supitcha Kaewma
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of the thesis were i) to improve the success of using sex-sorted semen and ii) to enhance the semen quality in low semen quality bulls and boars. Four experiments were conducted to test the hypothesis as 1) on-farm factors influence the success of sexed semen artificial insemination (AI) in cattle herds, 2) estrus synchronization protocols can be integrated in sex-sorted semen AI in cattle herd, 3) Lasia spinosa Thw. (LST) supplementation for improving semen quality in bull and boar sires with poor semen quality, 4) zinc supplementation for improving semen quality in subfertile boar. Study 1: Experiment 1, …