Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Animal Sciences (45)
- Life Sciences (45)
- Veterinary Infectious Diseases (3)
- Veterinary Microbiology and Immunobiology (2)
- Ecology and Evolutionary Biology (1)
-
- Environmental Sciences (1)
- Natural Resources Management and Policy (1)
- Natural Resources and Conservation (1)
- Other Environmental Sciences (1)
- Other Veterinary Medicine (1)
- Physical Sciences and Mathematics (1)
- Population Biology (1)
- Terrestrial and Aquatic Ecology (1)
- Veterinary Preventive Medicine, Epidemiology, and Public Health (1)
- Zoology (1)
- Publication Year
- Publication
- Publication Type
Articles 1 - 30 of 63
Full-Text Articles in Veterinary Medicine
Determination Of The Cecal Microbiota Profile Of Anadolu-T Broiler Breeder Chickens Using 16s Metagenomic Sequencing, Kürşat Teti̇k, İsmai̇l Özkan, Serpi̇l Kahya Demi̇rbi̇lek
Determination Of The Cecal Microbiota Profile Of Anadolu-T Broiler Breeder Chickens Using 16s Metagenomic Sequencing, Kürşat Teti̇k, İsmai̇l Özkan, Serpi̇l Kahya Demi̇rbi̇lek
Turkish Journal of Veterinary & Animal Sciences
The gut microbiota has significant effects on the performance, health status, and immune systems of chickens. The complex microbial community structure begins to form after chicks hatch with the intake of feed, water, and air into the digestive system. Disrupting the balance of this system can lead to the proliferation of pathogenic microorganisms. Until recently, studies on microbiota relied on traditional microbiological techniques that cultured only a small fraction of the microbial diversity. However, significant advancements in DNA sequencing and bioinformatics have enabled the identification of diverse bacterial populations in environmental samples. In this study, a total of 2000 broiler …
Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia
Molecular Identification Of Cryptosporidium Spp. Isolated From Dairy Calves In The Northern Region Of Algeria, Dadda Anes, Abdellah Mohamed Cherif, Aoun Leila, Chatanun Eamudomkarn, Khelef Djamel, Keeseon S Eom, Dongmin Lee, Barakaeli Abdieli Ndosi, Mohammed Mebarek Bia
The Thai Journal of Veterinary Medicine
Cryptosporidium spp. is the most frequent agent in Algeria that causes neonatal diarrhea in calves, with high symptomatic diarrhea in pre-weaned dairy calves. The study aims to measure the prevalence, distribution, and genotype of Cryptosporidium-infected calves in one of Algeria's high milk production areas (North Central and Northeast). From 35 dairy cattle farms were selected 172 (77 with diarrheic and 95 without diarrheic symptoms) calves aged less than 60 days. Twenty-three farms (65.71%) were detected with cryptosporidiosis using a modified Ziehl-Neelsen stain, whereby 68 calves (39.53%) were obtained positive. The Copro-ELISA test gave an individual prevalence of (37.66%, 22.11%) …
Phenotypic And Molecular Characterization And Antimicrobial Susceptibility Pattern Of Pasteurella And Mannheimia Species Obtained From Cattle And Sheep With Pneumonia, Eli̇f Karadeni̇z Pütür, Fati̇h Büyük, Yaren Ersoy
Phenotypic And Molecular Characterization And Antimicrobial Susceptibility Pattern Of Pasteurella And Mannheimia Species Obtained From Cattle And Sheep With Pneumonia, Eli̇f Karadeni̇z Pütür, Fati̇h Büyük, Yaren Ersoy
Turkish Journal of Veterinary & Animal Sciences
Among the heterogeneous Pasteurellaceae family, Pasteurella multocida and Mannheimia haemolytica are the pathogenic species that cause enzootic pneumonia in cattle and sheep. This study aimed to characterise Pasteurella and Mannheimia species obtained from pneumonia cases in cattle and sheep. 100 cattle and 100 sheep lungs diagnosed with infectious pneumonia as a result of histopathological examination were analyzed for Pasteurellaceae members by phenotypic methods, VITEK 2 Compact system and PCR. Antibiotic susceptibilities and phylogenetic analysis of the isolates were done by VITEK 2 Compact System and 16S rRNA-PCR, respectively. Seven Pasteurella multocida and 9 Mannheimia haemolytica were identified. All strains were …
Investigation Of Molecular Detection Rate And Associated Risk Factors Of Psittacine Beak And Feather Disease Virus In Psittaciformes In Iran, Mojtaba Khosravi, Shoreh Alian Samakkhah, Rahem Khoshbakht
Investigation Of Molecular Detection Rate And Associated Risk Factors Of Psittacine Beak And Feather Disease Virus In Psittaciformes In Iran, Mojtaba Khosravi, Shoreh Alian Samakkhah, Rahem Khoshbakht
Turkish Journal of Veterinary & Animal Sciences
Psittacine beak and feather disease virus (PBFDV) is a significant viral pathogen affecting the development of beak and feather cells, leading to feather abnormalities in avian species. This study aimed to investigate the molecular detection rate of PBFDV among Psittaciformes in Iran and to evaluate the influence of factors such as sex, species, season, and bird origin on PBFDV prevalence. Feather samples were collected from 1335 Psittaciformes, including Agapornis roseicollis, Pyrrhura molinae, Myiopsitta monachus, Aratinga solstitialis, Nymphicus hollandicus, Psittacula krameri, Psittacus erithacus, Poicephalus senegalus, Eos bornea, and Agapornis fischeri, and subjected to polymerase chain reaction (PCR) for PBFDV detection. The …
Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm
Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm
Turkish Journal of Veterinary & Animal Sciences
Genetic diseases play an increasingly important role in cattle breeding as breeding technologies such as artificial insemination has an inborn risk of disseminating deleterious genes, especially from high merit breeding sires. Cholesterol deficiency (CD) is a metabolic disorder first recognized in Holstein cattle in 2015. The condition is characterized by chronic diarrhea and unthriftiness in calves being homozygous for the defective allele. The prevalence of carriers of CD in Turkish cattle is at present unknown and therefore 700 heifers and cows of different breeds, including 300 Holsteins were genotyped. Of the tested Holsteins, 2.0% turned out to be carriers of …
Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak
Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak
Turkish Journal of Veterinary & Animal Sciences
In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …
Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey), Dogan Akca, Fatih Buyuk, Elif Celik, Aliye Gulmez Saglam, Salih Otlu, Serpil Dag, Ozgur Celebi, Mustafa Reha Coskun, Eray Buyuk, Emin Karakurt, Mitat Sahin
Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey), Dogan Akca, Fatih Buyuk, Elif Celik, Aliye Gulmez Saglam, Salih Otlu, Serpil Dag, Ozgur Celebi, Mustafa Reha Coskun, Eray Buyuk, Emin Karakurt, Mitat Sahin
The Thai Journal of Veterinary Medicine
This study aimed to investigate Listeria species in various samples taken from aborted sheep in Kars province and to determine the position of emerged strains in the phylogenetic tree. Milk (n= 229) and vaginal swab (n= 263) samples from aborted sheep and abomasum contents (n= 46) of aborted sheep fetuses were investigated by the culture and Polymerase Chain Reaction (PCR) methods. For the phylogenetic analysis of isolates, the 16S rRNA gene region was sequenced. In the culture, the Listeria agent was not isolated from any of the vaginal swabs or milk samples. Among the 46 fetal abomasum contents, a Listeria …
Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan, Sheng-Chang Chung, Ching-Dong Chang, Perng-Chih Shen, Wei Lin, Chiu-Huang Ting, Hung-Yi Wu
Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan, Sheng-Chang Chung, Ching-Dong Chang, Perng-Chih Shen, Wei Lin, Chiu-Huang Ting, Hung-Yi Wu
The Thai Journal of Veterinary Medicine
This study aimed to understand the related statistics of APV and PBFDV in parrots, such as the positive percentage and the distributions of various ages, seasons, breeds and areas, in order to obtain more information on the health care of captive parrots in Taiwan today. Blood samples were collected from parrots that were household pets and from commercial aviaries in the northern (e.g. Taipei), central (e.g. Taichung) and southern (e.g. Kaohsiung) Taiwan regions randomly. Collection targets were mainly small and medium species such as Psittacula krameri and others such as Psittacus erithacus. Sixty samples were collected every month and a …
Detection Of Rhodococcus Equi By Pcr From Foals And Determination Of Antimicrobial Susceptibility, Şükrü Kirkan, Uğur Parin, Hafi̇ze Tuğba Yüksel Dolgun, Eli̇f Oya Oral
Detection Of Rhodococcus Equi By Pcr From Foals And Determination Of Antimicrobial Susceptibility, Şükrü Kirkan, Uğur Parin, Hafi̇ze Tuğba Yüksel Dolgun, Eli̇f Oya Oral
Turkish Journal of Veterinary & Animal Sciences
The scope of this research was determination of Rhodococcus equi isolates and antimicrobial susceptibilities in nasal swab specimen taken from animals between 0 and 6 months of age period. R. equi was identified from 10 isolates (11.1%) by automatic identification device. The verification of isolates were conducted by 16S ribosomal RNA PCR. VapA gene presence was detected in two (20%) isolates and multidrug resistance was identified against 11 antimicrobials. The antimicrobial resistance to macrolides and rifampin is arising worldwide since the excessive usage of the chemotherapeutics in various breeding farms just by evaluating monitoring outcomes. These results exhibit the presence …
Peptide Elisa And Fret-Qpcr Identified A Significantly Higher Prevalence Of Chlamydia Suis In Domestic Pigs Than In Feral Swine From The State Of Alabama, Usa, Md Monirul Hoque, Folasade Adekanmbi, Subarna Barua, Kh. Shamsur Rahman, Virginia Aida, Brian Anderson, Anil Poudel, Anwar Kalalah, Sara Bolds, Steven Madere, Steven Kitchens, Stuart Price, Vienna Brown, B. Graeme Lockaby, Constantinos S. Kyriakis, Bernhard Kaltenboeck, Chengming Wang
Peptide Elisa And Fret-Qpcr Identified A Significantly Higher Prevalence Of Chlamydia Suis In Domestic Pigs Than In Feral Swine From The State Of Alabama, Usa, Md Monirul Hoque, Folasade Adekanmbi, Subarna Barua, Kh. Shamsur Rahman, Virginia Aida, Brian Anderson, Anil Poudel, Anwar Kalalah, Sara Bolds, Steven Madere, Steven Kitchens, Stuart Price, Vienna Brown, B. Graeme Lockaby, Constantinos S. Kyriakis, Bernhard Kaltenboeck, Chengming Wang
United States Department of Agriculture Wildlife Services: Staff Publications
Chlamydia suis is an important, highly prevalent, and diverse obligate intracellular pathogen infecting pigs. In order to investigate the prevalence and diversity of C. suis in the U.S., 276 whole blood samples from feral swine were collected as well as 109 fecal swabs and 60 whole blood samples from domestic pigs. C. suis-specific peptide ELISA identified anti-C. suis antibodies in 13.0% of the blood of feral swine (26/276) and 80.0% of the domestic pigs (48/60). FRET-qPCR and DNA sequencing found C. suis DNA in 99.1% of the fecal swabs (108/109) and 21.7% of the whole blood (13/60) of …
Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu
Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu
The Thai Journal of Veterinary Medicine
Salmonellaspp. can be widely spread by air or contaminated food. There are sixcommon pathogenic Escherichia coli whichaccount for 4–8% of all E. coli pathogens. Amongstthem, E. coliO157 is the most commonlyknown. In this study, we investigated the prevalence of diarrhoeapathogens, referred to as Salmonellaspp.andEscherichia coli(E. coli), in pet dogs in western Taiwan using the conventionalpolymerase chain reaction (PCR). The related analysis between the prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. Faecalsamples were collected from240dogs which had symptoms of diarrhoeaand had been examined byveterinaryhospitals from January 2015 to January 2017. The …
An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun
An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun
Turkish Journal of Veterinary & Animal Sciences
A 6-year-old Simental-Swiss Brown cross-bred cow, which aborted in the first trimester of the gestation and involuntarily presented anthrax bacilli around the udder, was subjected to this study. The cow was vaccinated with an attenuated anthrax vaccine (Bacillus anthracis 34 F2) 6 months ago and had normal physical examination findings. Samples taken 10 days after the abortion were representative of all possible spaces that could carry the bacteria such as milk, blood, environment, and swabs from rectum, udder and tits of an infected cow. Microbiological examination was carried out by staining the smears with Azure and in vitro culture of …
Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski
Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive …
Evaluation Of Larval Culture And Conventional Pcr Methods For The Detection Ofstrongylus Vulgaris In Equines Of Iran, Alireza Alborzi, Sara Larki, Abbas Zeinali
Evaluation Of Larval Culture And Conventional Pcr Methods For The Detection Ofstrongylus Vulgaris In Equines Of Iran, Alireza Alborzi, Sara Larki, Abbas Zeinali
Turkish Journal of Veterinary & Animal Sciences
Strongylus vulgaris (S. vulgaris) is known as the equin's most pathogenic nematode due to the extraintestinal migration in mesenteric arterial vessels. Since a few kinds of S. vulgaris can threat the animals' health and working efficiency, high precision and early detection of strongylid nematodes is essential. Traditionally, the larval coproculture method was used frequently for identification of strongylid species. The technique of larval culture is costly, time consuming, and does not have sufficient accuracy and reliability. Here, we critically evaluated the polymerase chain reaction (PCR) with larval culture methods for the detection of S. vulgaris eggs in fecal samples. To …
Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
A normalisationof targeted genes using the appropriate reference genes is necessary for reliable quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. This study aimed to investigate the appropriate reference genes and the numbers of the genes required for qRT-PCR in canine pulmonary arteries. Pulmonary arteries were collected from twenty-three dogs at necropsy. Candidate reference genesincluding ribosomal protein L32 (RPL32), ribosomal protein L13a (RPL13A), ribosomal protein S18 (RPS18), TATA box binding protein (TBP), beta-2-microglobulin (B2M), ribosomal protein S5 (RPS5), ribosomal protein S19 (RPS19) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pulmonary arteries were examined by qRT-PCR and analysed using four major algorithms, including …
Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu
Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu
The Thai Journal of Veterinary Medicine
In this study, we investigated the prevalence of Diarrhea pathogens in pet dogs referred to as Canine Parvovirus (CPV) and Canine Rotavirus (CRV)in western Taiwan using conventional polymerase chain reaction (PCR).Fecalsampleswere collected from240 dogs which have the symptomsofdiarrhea examined bythe veterinaryhospitals from March 2015 to March 2017.The PCR sensitivityof total DNAs extracted from 0.1g fecal samples rangedfrom 10ng to100 ng. The prevalence of CPV and CRV infections were23.3% (56/240) and 9.2% (22/240)respectively.The related analysis between prevalence rates and the epidemiological data of pet dogs were correlated with the age, season,area, vaccinationandbreed. The results showed that both the diseases have the …
Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi
Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi
The Thai Journal of Veterinary Medicine
This cross-sectional study aimed to determine the prevalence of Trypanosoma evansi infection and its associated risk factors in camels in the Jazan region, southwestern Saudi Arabia. A total of 679 blood samples were collected from three local breeds of camel – Awadia, Awarkia and Sawahelle – in 11 randomly selected governorates of Jazan region. Blood samples were screened by Giemsa-stained blood film (GSBF) and polymerase chain reaction (PCR) techniques. The overall prevalence of T. evansi was 30.9% (210/679, CI: 27.6–34.5%) by PCR, whereas a prevalence of 22.2% (151/679, CI: 19.3–25.5%) was revealed by GSBF. The highest prevalence was identified in …
Incidence Of Brucellosis In Aborted Animals And Occupationally Exposed Veterinary Professionals Of Bannu, Khyber Pakhtunkhwa, Pakistan, Azmatullah Khan, Muhammad Shafee, Naimatullah Khan, Attaur Rahman, Rafiullah Rafiullah, Ijaz Ali, Imad Khan, Sadeeq Ur Rahman
Incidence Of Brucellosis In Aborted Animals And Occupationally Exposed Veterinary Professionals Of Bannu, Khyber Pakhtunkhwa, Pakistan, Azmatullah Khan, Muhammad Shafee, Naimatullah Khan, Attaur Rahman, Rafiullah Rafiullah, Ijaz Ali, Imad Khan, Sadeeq Ur Rahman
The Thai Journal of Veterinary Medicine
Brucellosis in animals is a serious global health concern mainly due to its zoonotic nature and association with heavy economic losses. Brucellosis has been reported randomly from different parts of Pakistan; however, the prevalence of brucellosis in animals and humans in Bannu District, as well as overall epidemiological data of the country, is missing. The present study reports on the occurrence of brucellosis in domestic animals and its cross-circulation among occupationally exposed human population in Bannu District, Khyber Pakhtunkhwa, Pakistan. A total of 223 (animals=100, animal handlers=73, veterinary staff=50) serum samples were collected and subjected to serum plate agglutination test …
The Pathology Of Tuberculosis Lesions In Allergic Skin Test (Ppd Tuberculin) Positive Cows And Detection Of Tuberculosis Agents With Pcr In Milk Samples, Çi̇ğdem Tuncay, Fati̇h Hati̇poğlu
The Pathology Of Tuberculosis Lesions In Allergic Skin Test (Ppd Tuberculin) Positive Cows And Detection Of Tuberculosis Agents With Pcr In Milk Samples, Çi̇ğdem Tuncay, Fati̇h Hati̇poğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine tuberculosis (Tb) agents in milk and the pathologic changes in the tissues and organs of 100 PPD Tb-positive cows that were intended for slaughter. Milk samples were taken before slaughtering and PCR analysis was conducted to detect the Tb agent. In postmortem examinations, the lungs, liver, mammary glands, spleen, kidneys, and heart were collected for macroscopic and histopathological examinations. Out of the 100 PPD positive cows, lesions of Tb were observed in 75 cows. In the microscopic examination, Tb was identified in the lungs (51 cases), liver (61 cases), and in both …
Identification Of Sarcocystis Spp. By Polymerase Chain Reaction And Microscopic Examination In Various Beef Products (Minced Meat, Meatballs, Fermented Sausage), Ufuk Kamber, Mükremi̇n Özkan Arslan, Güven Gülbaz, Gencay Taşkin Taşçi, Ati̇la Akça
Identification Of Sarcocystis Spp. By Polymerase Chain Reaction And Microscopic Examination In Various Beef Products (Minced Meat, Meatballs, Fermented Sausage), Ufuk Kamber, Mükremi̇n Özkan Arslan, Güven Gülbaz, Gencay Taşkin Taşçi, Ati̇la Akça
Turkish Journal of Veterinary & Animal Sciences
Sarcocystis species are the most commonly encountered parasitic organisms in animals. Sarcocystis hominis (Sarcocystis bovihominis), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been established that, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes food poisoning. Therefore, this study was undertaken in order to determine the extent to which Sarcocystis species are present in minced meat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, were collected in …
Identification Of Mycobacteria From Unhealthy And Apparently Healthy Aquarium Fish Using Both Conventional And Pcr Analyses Of Hsp65 Gene, Mahdi Seyfahmadi, Seyyed Reza Moaddab, Azar Sabokbar
Identification Of Mycobacteria From Unhealthy And Apparently Healthy Aquarium Fish Using Both Conventional And Pcr Analyses Of Hsp65 Gene, Mahdi Seyfahmadi, Seyyed Reza Moaddab, Azar Sabokbar
The Thai Journal of Veterinary Medicine
Seventy-eight unhealthy and forty-two apparently healthy looking freshwater aquarium fish belonging to twenty-six different species were collected from local aquarium stores in different cities and were investigated for the presence of mycobacteria in north-western Iran by culture and molecular methods. Using the culture method, Mycobacterium sp. was isolated from 31.6% of the fish (38 cases), while it was obtained in 30.8% (37 cases) by polymerase chain reaction (PCR) assay for a 439 bp fragment of the heat shock protein 65kD gene (hsp65). Acid-fast bacilli (AFB) were seen on the smears of 27 samples (22.5%) by direct microscopic examination method of …
Isolation Of Virulent Rhodococcus Equi From Arabian Horses In Şanlıurfa, Turkey, Osman Yaşar Tel, Oktay Keski̇n, Sevi̇l Erdenli̇ğ Gürbi̇lek
Isolation Of Virulent Rhodococcus Equi From Arabian Horses In Şanlıurfa, Turkey, Osman Yaşar Tel, Oktay Keski̇n, Sevi̇l Erdenli̇ğ Gürbi̇lek
Turkish Journal of Veterinary & Animal Sciences
The purposes of this study were to investigate plasmid profiles of the isolated virulent (VapA positive) agents by RFLP and to determine the quantitative aspects of Rhodococcus equi in the feces of adult horses and foals, as well as in soil. The frequency of detection of R. equi and virulent R. equi in fecal samples and soil was studied from 20 farms in the Şanlıurfa region. A total of 315 samples were examined. Nasal swabs from 25 foals with pneumonia were also analyzed. R. equi was isolated and identified from 263 (83.4%) samples originating from 15 out of 20 farms. …
Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r
Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates …
Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç
Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç
Turkish Journal of Veterinary & Animal Sciences
Canine leishmaniasis (CanL) is a protozoal disease caused by Leishmania species. Dogs play an important role as a reservoir. The aim of this study is to determine the prevalence of CanL by molecular and serological techniques among stray dogs living in kennels in Aydın, Manisa, and İzmir, located in western Turkey. Blood samples were collected from a total of 191 dogs and analyzed using indirect immunofluorescence antibody test (IFAT) and polymerase chain reaction (PCR). The isolates that tested positive using PCR were further analyzed by real-time PCR for species identification and confirmation. A total of 21 (10.99%) dogs were found …
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. Coli isolated from cattle, goats, sheep, cats, and dogs in Turkey. Twenty nonreplicate E. Coli isolates (chosen on the basis of RAPD pattern) from food-producing animals were selected for the study. To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates. PCR and qRT-PCR were used to characterize the presence and expression levels of known molecular mechanisms of …
Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani
Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani
The Thai Journal of Veterinary Medicine
Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …
Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan
Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed …
First Detection And Molecular Identification Of Babesia Gibsoni In Two Dogs From The Aydın Province Of Turkey, Nuran Aysul, Kerem Ural, Bülent Ulutaş, Hasan Eren, Tüli̇n Karagenç
First Detection And Molecular Identification Of Babesia Gibsoni In Two Dogs From The Aydın Province Of Turkey, Nuran Aysul, Kerem Ural, Bülent Ulutaş, Hasan Eren, Tüli̇n Karagenç
Turkish Journal of Veterinary & Animal Sciences
Small (1-3 µm in diameter, ring, oval, or comma shaped) piroplasms suggestive of Babesia spp. were observed upon microscopic examination of Giemsa-stained peripheral blood smears from a 3.5-year-old American Pit Bull Terrier with clinical signs of pyrexia, weakness, mucous membrane pallor, and depression living in Aydın, Turkey, in February, 2009. To confirm the presence and to identify the species of Babesia spp., a polymerase chain reaction (PCR) technique was performed using primers amplifying a 670 bp fragment of the 18S rRNA gene of Babesia gibsoni and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the …
Clostridium Botulinum In Honey: Prevalence And Antibiotic Susceptibility Of Isolated Strains, Ahmet Koluman, Berfi̇n Meli̇koğlu Gölcü, Okan Deri̇n, Si̇bel Özkök, Fabrizio Anniballi
Clostridium Botulinum In Honey: Prevalence And Antibiotic Susceptibility Of Isolated Strains, Ahmet Koluman, Berfi̇n Meli̇koğlu Gölcü, Okan Deri̇n, Si̇bel Özkök, Fabrizio Anniballi
Turkish Journal of Veterinary & Animal Sciences
Clostridium botulinum and rare strains of C. butyricum and C. baratii produce an extremely potent toxin, the botulinum neurotoxin (BoNT). Infant botulism is significant for both its high mortality rates and for the sophisticated treatment that it requires. Isolation and identification of C. botulinum according to standards depend on mouse bioassays to determine toxin-producing ability of strains. Polymerase chain reaction (PCR) is used to detect the types of toxins expressed by bacteria. Since honey is an important source of infant botulism, we determined the types of toxins secreted by C. botulinum strains and their antibiotic resistance. In this study, the …
Indel Mutation Of The Add1/Srebp-1c Gene In The South Anatolian Red And East Anatolian Red Cattle Breeds, Kemal Özdem Öztabak, Iraz Akiş Akad, Feraye Esen Gürsel, Ati̇la Ateş, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
Indel Mutation Of The Add1/Srebp-1c Gene In The South Anatolian Red And East Anatolian Red Cattle Breeds, Kemal Özdem Öztabak, Iraz Akiş Akad, Feraye Esen Gürsel, Ati̇la Ateş, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
Turkish Journal of Veterinary & Animal Sciences
The present study was designed to determine genotypes of adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. Fifty cattle from the SAR breed and 43 cattle from the EAR breed were used. Intron 7 of the ADD1/SREBP1c gene containing an 84 bp indel mutation was amplified by polymerase chain reaction (PCR). The number of SS genotyped individuals with the 84 bp deletion associated with fatty acid composition and growth traits were very low in both breeds. The frequency of the LL genotype with the 84 bp …