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Veterinary Medicine Commons

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2008

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Articles 1 - 30 of 189

Full-Text Articles in Veterinary Medicine

Reducing The Public Health Impact Of Bovine Tuberculosis By Controlling Disease Transmission Between Cattle And White-Tailed Deer In Northwestern Minnesota, Barbara Knust Dec 2008

Reducing The Public Health Impact Of Bovine Tuberculosis By Controlling Disease Transmission Between Cattle And White-Tailed Deer In Northwestern Minnesota, Barbara Knust

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis, caused by infection with Mycobacterium bovis, is a re-emerging zoonotic disease. It has staged a comeback by establishing infections in wildlife and cattle, creating the potential for human disease in locations where it was thought to be under control. In northwestern Minnesota, infected cattle and white-tailed deer were first discovered in 2005. A major bovine tuberculosis eradication campaign is underway in the state, with multiple efforts employed to control M. bovis infection in both cattle and deer populations. In order to effectively eradicate bovine tuberculosis in Minnesota, there is a need for better understanding of the …


Comparative Effects Of Zinc Methionylglycinate And Zinc Sulfate On Hair Coat Characteristics And Zinc Concentration In Plasma, Hair, And Stool Of Dogs, Uttra Jamikorn, Thanisara Preedapattarapong Dec 2008

Comparative Effects Of Zinc Methionylglycinate And Zinc Sulfate On Hair Coat Characteristics And Zinc Concentration In Plasma, Hair, And Stool Of Dogs, Uttra Jamikorn, Thanisara Preedapattarapong

The Thai Journal of Veterinary Medicine

There are two form of Zn supplement used in commercial dog foods, organic and inorganic forms. These forms can influence Zn absorption and utilization of the animals. The goal of the current study was to evaluate the effects of zinc methionylglycinate (ZnMG) compared to zinc sulfate (ZnSO4) supplementations in commercial dog foods on haircoat characteristics, and Zn concentration in plasma, hair, and stool of the dogs. Eight mature female beagles were randomly divided into two groups of four dogs each. A Cross-over design was used for this study. The treatments composed of 120 ppm Zn supplement of either ZnMG or …


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2008

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


The Efficacy Of Tilmicosin Against Brolier Chickens Infected With Mycoplasma Gallisepticum Isolated In Thailand, Somsak Pakpinyo, Visut Rawiwet, Warod Buranasiri, Supasakol Jaruspibool Dec 2008

The Efficacy Of Tilmicosin Against Brolier Chickens Infected With Mycoplasma Gallisepticum Isolated In Thailand, Somsak Pakpinyo, Visut Rawiwet, Warod Buranasiri, Supasakol Jaruspibool

The Thai Journal of Veterinary Medicine

Sixty 1-day-old-female broiler chickens were divided into groups as follows. Thirty birds were randomly selected to be bled from jugular vein for Mycoplasma gallisepticum (MG) antibody assay and swabbed at yolk sac for the detection of MG DNA by using PCR. The remaining birds were divided into three groups, 10 birds per group as follows. Group 1 was a sham negative control. Group 2 received MG serving as a positive control, whereas group 3 received MG and simultaneously treated with tilmicosin incorporated into drinking water. Each bird received MG that was injected into the left thoracic airsac, with 0.1 ml …


Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu Dec 2008

Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Estrogens are known to be an important modulator for regulation of the reproductive function in female mammals. The specific receptor of estrogens in the buffalo reproductive tract, particularly in the oviduct, has not been explored yet in the expression pattern. This study was, therefore, undertaken to investigate the localization of the estrogen receptor alpha (ERα) in the oviduct of Thai swamp buffalo at the follicular and late luteal phases of the estrous cycle. The oviducts were collected from swamp buffalo genital tracts at the local abattoir and separated into the uterotubal junction (UTJ), isthmus, ampulla and infundibulum. Tissue distribution of …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn Dec 2008

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep Dec 2008

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Follicular Dynamics Following Estrus Synchronization In Swamp Buffalo Cows (Bubalus Bubalis), Akachart Promdireg, Giorgio Antonio Presicce, Fabio De Rensis, Jinda Singlor, Mongkol Techakumphu Dec 2008

Follicular Dynamics Following Estrus Synchronization In Swamp Buffalo Cows (Bubalus Bubalis), Akachart Promdireg, Giorgio Antonio Presicce, Fabio De Rensis, Jinda Singlor, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The objective of this study was to elucidate ovarian follicular dynamics in swamp buffalo cows (Bubalus bulalis) following a protocol for estrus synchronization during hot as low breeding season (March to June) and cool as high breeding season (November to February). Nine pluriparous buffalo cows received a progesterone ear implant for 10 days together with the administration of a luteolytic dose of PGF2α at the time of implant removal to start the estrus cycle. Human Chorionic Gonadotrophin was administered whenever ovulation did not occur within the first five days following implant removal. Daily ultrasound monitoring and blood collection for progesterone …


Effects Of The Dietary Inclusion Of Fish Meal, Rock Phosphate And Roxarsone On Arsenic Residues In Tissues Of Broilers, Kris Angkanaporn, Kriengsak Daengprom, Chutarat Kiratisehwe Dec 2008

Effects Of The Dietary Inclusion Of Fish Meal, Rock Phosphate And Roxarsone On Arsenic Residues In Tissues Of Broilers, Kris Angkanaporn, Kriengsak Daengprom, Chutarat Kiratisehwe

The Thai Journal of Veterinary Medicine

The effect of dietary ingredients such as fish meal, rock phosphate and roxarsone on the accumulation of arsenic residues in breast meat, liver and heart was examined in Arbor Acre broilers from days 1-43 of age. Broilers receiving diets containing fish meal had a significantly greater amount of arsenic in breast muscle than other groups. There was a positive correlation between muscular arsenic concentration and dietary fish meal levels. Withdrawal of fish meal for a week prior to slaughter reduced the muscular deposition of arsenic. Chicks receiving roxarsone had significantly higher arsenic level in the liver compared to other groups …


Blood Haematological-Cholesterol Profile And Antibody Titer Response Of Broilers With Added Probiotic Containing Both Bacteria And Yeast Or An Antibiotic In Drinking Water, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong, Vivat Chavananikul Dec 2008

Blood Haematological-Cholesterol Profile And Antibody Titer Response Of Broilers With Added Probiotic Containing Both Bacteria And Yeast Or An Antibiotic In Drinking Water, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

An experiment was conducted to investigate the haematological and cholesterol profiles and antibody titer responses to both Newcastle disease (ND) and Infectious bronchitis (IB) vaccines in broiler chickens after drinking water supplemented with either a commercial probiotic (an Active Elements®; AE) containing Lactobacillus plantarum and Saccharomyces cerevisiae, an antibiotic Chlortetracycline (CTC), or the probiotic plus the antibiotic. Four hundred one-day-old male Cobb broiler chicks were randomly allocated to 4 treatment groups designated as follow: a non-treated control (T1), a 0.05% CTC (T2), a 1:500 AE (T3), and a combination of 0.05% CTC and 1:500 AE (T4), respectively. The chicks were …


Ophthalmology Snapshot, Nalinee Tuntivanich Dec 2008

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Role Of The Veterinarian On Animal Welfare, Achariya Sailasuta Dec 2008

Role Of The Veterinarian On Animal Welfare, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.


Differential Gene Expression Associated With Postnatal Equine Articular Cartilage Maturation, Michael J. Mienaltowski, Liping Huang, Arnold J. Stromberg, James N. Macleod Nov 2008

Differential Gene Expression Associated With Postnatal Equine Articular Cartilage Maturation, Michael J. Mienaltowski, Liping Huang, Arnold J. Stromberg, James N. Macleod

Veterinary Science Faculty Publications

BACKGROUND: Articular cartilage undergoes an important maturation process from neonate to adult that is reflected by alterations in matrix protein organization and increased heterogeneity of chondrocyte morphology. In the horse, these changes are influenced by exercise during the first five months of postnatal life. Transcriptional profiling was used to evaluate changes in articular chondrocyte gene expression during postnatal growth and development.

METHODS: Total RNA was isolated from the articular cartilage of neonatal (0-10 days) and adult (4-5 years) horses, subjected to one round of linear RNA amplification, and then applied to a 9,367-element equine-specific cDNA microarray. Comparisons were made with …


Behavioural Analysis Of A Nociceptive Event In Fish: Comparisons Between Three Species Demonstrate Specific Responses, Siobhan C. Reilly, John P. Quinn, Andrew R. Cossins, Lynne U. Sneddon Nov 2008

Behavioural Analysis Of A Nociceptive Event In Fish: Comparisons Between Three Species Demonstrate Specific Responses, Siobhan C. Reilly, John P. Quinn, Andrew R. Cossins, Lynne U. Sneddon

Sentience Collection

Nociception is the sensory mechanism by which potentially harmful stimuli are detected in animals and humans. The behavioural responses to noxious stimulation have been studied in two fish species thus far. However, since species-specific differences are seen in mammals, more species need to be examined to determine whether nociceptive responses are generic in fish. The present study investigated the behavioural and respiratory response to an acute noxious or potentially painful stimulus in common carp (Cyprinus carpio), zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss). Behavioural parameters such as frequency of swimming, use of cover and any anomalous behaviour were measured …


Antibody Responses Of Cervids (Cervus Elaphus) Following Experimental Mycobacterium Bovis Infection And The Implications For Immunodiagnosis, Noel P. Harrington, Om P. Surujballi, John F. Prescott, J. Robert Duncan, W. Ray Waters, Konstantin Lyashchenko, Rena Greenwald Nov 2008

Antibody Responses Of Cervids (Cervus Elaphus) Following Experimental Mycobacterium Bovis Infection And The Implications For Immunodiagnosis, Noel P. Harrington, Om P. Surujballi, John F. Prescott, J. Robert Duncan, W. Ray Waters, Konstantin Lyashchenko, Rena Greenwald

Michigan Bovine Tuberculosis Bibliography and Database

Captive and free-ranging wildlife animals are implicated in the maintenance and transmission of bovine tuberculosis and therefore pose a significant obstacle to eradication of the disease from domestic livestock. The current antemortem diagnostic method, the intradermal tuberculin skin test, is impractical for routine use with many wild animals. Antibody-based assays are particularly attractive because the animals are handled only once and immediate processing of the sample is not required. This report characterizes the antibody responses of red deer-elk hybrids (Cervus elaphus) against Mycobacterium bovis and subsequently evaluates the diagnostic performance of select antigens in a rapid-test format. Sequential …


Animal Health Matters, David H. Zeman Oct 2008

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director’s Message [Page] 1-ADRDL Receives Full Accreditation from AAVLD Diagnostic News [Page] 1-An Unusual Presentation of Blackleg in a South Dakota Beef Herd [Page] 2- Holiday Hours [Page] 3- Selected Abstracts, SDSU ADRDL Presentations, 2008 Meeting [Page] 3- Abomasitis in Beef Calves [Page] 3- Test Validation: What it is & why it’s important to you and your clients [Page] 3- Wildlife Zoonoses and the Veterinarian [Page] 3- Great Lakes Fish Die Off Due to VHS Virus Research News [Page] 4- Research Spotlight: Dr. Feng Li Extension News [Page] 5- Communicating with Media [Page] 5- Summary of BVDV-PI Testing at SDSU …


Got Controversy - Milk Does, Margaret Sova Mccabe Oct 2008

Got Controversy - Milk Does, Margaret Sova Mccabe

Law Faculty Scholarship

This article analyzes ongoing controversy over how to best label rBST-free milk. Recombinant bovine somatotropin is a genetically engineered drug administered by some farmers to their dairy herds to increase milk production. FDA first approved its use in 1994, despite great controversy. The FDA also issued labeling guidelines that allowed voluntary disclosure of rBST-free milk, so long as it carried the disclaimer that no difference could be detected between milk produced with rBST and rBST-free. The controversy continues today as consumers express a preference for rBST-free milk and many rBST-free producers label their milk this way. "Conventional" milk (with rBST) …


Missense Mutation In Exon 2 Of Slc36a1 Responsible For Champagne Dilution In Horses, Deborah Cook, Samantha Brooks, Rebecca Bellone, Ernest Bailey Sep 2008

Missense Mutation In Exon 2 Of Slc36a1 Responsible For Champagne Dilution In Horses, Deborah Cook, Samantha Brooks, Rebecca Bellone, Ernest Bailey

Veterinary Science Faculty Publications

Champagne coat color in horses is controlled by a single, autosomal-dominant gene (CH). The phenotype produced by this gene is valued by many horse breeders, but can be difficult to distinguish from the effect produced by the Cream coat color dilution gene (CR). Three sires and their families segregating for CH were tested by genome scanning with microsatellite markers. The CH gene was mapped within a 6 cM region on horse chromosome 14 (LOD = 11.74 for theta = 0.00). Four candidate genes were identified within the region, namely SPARC [Secreted protein, acidic, cysteine-rich (osteonectin)], SLC36A1 (Solute Carrier 36 family …


Intraretinal Signaling By Ganglion Cell Photoreceptors To Dopaminergic Amacrine Neurons, Dao-Qi Zhang, Kwoon Y. Wong, Patricia J. Sollars, David M. Berson, Gary E. Pickard, Douglas G. Mcmahon Sep 2008

Intraretinal Signaling By Ganglion Cell Photoreceptors To Dopaminergic Amacrine Neurons, Dao-Qi Zhang, Kwoon Y. Wong, Patricia J. Sollars, David M. Berson, Gary E. Pickard, Douglas G. Mcmahon

School of Veterinary and Biomedical Sciences: Faculty Publications

Retinal dopaminergic amacrine neurons (DA neurons) play a central role in reconfiguring retinal function according to prevailing illumination conditions, yet the mechanisms by which light regulates their activity are poorly understood. We investigated the means by which sustained light responses are evoked in DA neurons. Sustained light responses were driven by cationic currents and persisted in vitro and in vivo in the presence of L-AP4, a blocker of retinal ON-bipolar cells. Several characteristics of these L-AP4-resistant light responses suggested that they were driven by melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs), including long latencies, marked poststimulus persistence, and a peak …


Questions And Answers: Keeping Herds Free From Bovine Tuberculosis And Protecting The Food Supply Sep 2008

Questions And Answers: Keeping Herds Free From Bovine Tuberculosis And Protecting The Food Supply

Michigan Bovine Tuberculosis Bibliography and Database

Questions:
What can I do to keep my herd bovine tuberculosis (TB)-free?
How can I make sure that the animals I buy come from a TB-free herd?
How can I make sure that the animals I buy have not been exposed to a TB-infected herd?
Are new animals introduced to my stockyard a threat?
How do I protect calves from disease exposure?
If there is a TB outbreak in my area, are there any additional biosecurity measures, above and beyond best practices that I should take to reduce the chance of my cows getting the disease?
What biosecurity measures can …


Questions And Answers: Bovine Tuberculosis Signs And Symptoms Sep 2008

Questions And Answers: Bovine Tuberculosis Signs And Symptoms

Michigan Bovine Tuberculosis Bibliography and Database

Questions:

What is bovine tuberculosis (TB)?
What animals and species can it affect?
What are the symptoms of TB?
What should I do if my animals show these symptoms?
How does TB get into cattle herds?
Are dairies more susceptible to TB?
How does APHIS test for TB?
How many animals in the United States are tested for TB each year?
Typically, when are animals tested for TB?
Does wildlife spread TB to commercial cattle? W
hat is the risk of TB transmission from local wildlife?
How is TB being managed in wildlife?
How can I find out if the …


Treatment Of Infected Open Wounds On Two Dogs Using A Film Dressing Of Polysaccharide Extracted From The Hulls Of Durian (Durio Zibethinus Murr.): Case Report, Piyarat Chansiripornchai, Sunanta Pongsamart Sep 2008

Treatment Of Infected Open Wounds On Two Dogs Using A Film Dressing Of Polysaccharide Extracted From The Hulls Of Durian (Durio Zibethinus Murr.): Case Report, Piyarat Chansiripornchai, Sunanta Pongsamart

The Thai Journal of Veterinary Medicine

There have been many reports on the efficacy of the polysaccharide peptidoglycan (PG) isolated from durian hulls in assisting wound healing in pig, dog, and cat skin. In this report, a two - year - old and a three -year - old female mongrel dogs with infected open wounds and skin necrosis were treated with PG film dressing. Complete wound healing in both dogs occurred within 14 weeks of treatment. PG film dressing may effectively promotes wound healing. The results show the benefit of using PG film dressings in pharmaceutical and veterinary applications.


Let’S Celebrate 30th Anniversary Of The Federation Of The Asian Veterinary Associations, Achariya Sailasuta Sep 2008

Let’S Celebrate 30th Anniversary Of The Federation Of The Asian Veterinary Associations, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Sep 2008

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep Sep 2008

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn Sep 2008

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrastructures Of Red Blood Cells, White Blood Cells And Platelets In Four Species Of Macaques In Thailand, Kamol Sakulwira, Wimon Pothiwong, Pakorn Prachammuang, Siripen Vethchagarun Sep 2008

Ultrastructures Of Red Blood Cells, White Blood Cells And Platelets In Four Species Of Macaques In Thailand, Kamol Sakulwira, Wimon Pothiwong, Pakorn Prachammuang, Siripen Vethchagarun

The Thai Journal of Veterinary Medicine

The structure of blood cells and platelets in 11 macaques (2 stump-tailed macaques, 5 long-tailed macaques, 1 rhesus macaque and 3 pig-tailed macaques) were studied using light and transmission electron microscopy (TEM). Erythrocytes were non-nucleated, biconcave disks without cytoplasmic organelles. Neutrophils had numerous small specific granules. Eosinophils contained specific granules with a dense matrix and a pale crystalloid body. Basophils revealed large round specific granules. Lymphocytes possessed scant cytoplasm with some mitochondria and azurophilic granules. Monocytes contained a large number of mitochondria, azurophilic granules and endoplasmic reticulum. Platelets were represented by anucleation and they had several different organelles in the …


The Correlation Between Plasma L-Carnitine And Growth Performance In Suckling Pigs In Respect Of Mrna Expression Of A Carnitine Transporter, Octn2, In Lactating Porcine Mammary Tissues, Boonrit Thongsong, Sarinee Kalandakanond-Thongsong Sep 2008

The Correlation Between Plasma L-Carnitine And Growth Performance In Suckling Pigs In Respect Of Mrna Expression Of A Carnitine Transporter, Octn2, In Lactating Porcine Mammary Tissues, Boonrit Thongsong, Sarinee Kalandakanond-Thongsong

The Thai Journal of Veterinary Medicine

Two objectives of this study were to investigate the weekly change in plasma L-carnitine concentration during the 28 day suckling period and to determine the relative mRNA expression level of a L-carnitine transporter, OCTN2, in porcine mammary tissue at early and peak lactation. The results showed that the L-carnitine concentration of piglets after giving birth was 3.94±0.13 mg/L. L-carnitine levels on day 7 were significantly decreased (p<0.05) compared to those on the 1st day. On day 14, L-carnitine level increased significantly (p<0.05) to the concentration of 3.99±0.55 mg/L and maintained this level until weaning day. As with the increase in plasma L-carnitine concentration 1 week following suckling age, the body weight also increased significantly (p<0.05). The linear correlation of suckling ages and L-carnitine levels has a significant and positive correlation (R2Adj=0.1328, p<0.05). The mRNA expression of OCTN2 found in these lactating sow mammary tissues showed the relative abundance of this carnitine transporter. It tended to be decreased in lactating mammary tissue of sows at their peak compared to those at early lactation (p=0.065). Therefore, the significant change or maintenance in plasma L-carnitine concentration and body weight gain during suckling period provided an insight into the effects of L-carnitine in suckling piglets. Additionally, the mRNA expression of OCTN2 in the lactating mammary cells will be further investigated in cellular and molecular regulatory mechanisms to transport L-carnitine from dams to their piglets via the mammary glands.


Effect Of Pentoxifylline On The Motility Characteristics And Viability Of Spermatozoa In Asian Elephants (Elephas Maximus) With Low Semen Quality, Nikorn Thongtip, Jumnian Saikhun, Sittidet Mahasawangkul, Kornchai Kornkaewrat, Piyawan Suthanmapinanh, Anuchai Pinyopummin Sep 2008

Effect Of Pentoxifylline On The Motility Characteristics And Viability Of Spermatozoa In Asian Elephants (Elephas Maximus) With Low Semen Quality, Nikorn Thongtip, Jumnian Saikhun, Sittidet Mahasawangkul, Kornchai Kornkaewrat, Piyawan Suthanmapinanh, Anuchai Pinyopummin

The Thai Journal of Veterinary Medicine

To investigate the effects of pentoxifylline (PTX) to enhance the motility and fertilization capacity of semen samples with the low-motile sperm in Asian elephants, fourteen semen collection attempts in 9 elephant bulls by manual stimulation were undertaken and eleven ejaculates fitted the criteria of investigation (0-30% motility). They were divided into two groups: poor-motile (0-9% motility) and low-motile (10-30% motility) sperm groups. Fresh semen samples were divided as a control group and 3 experimental groups that were supplemented with PTX at a final concentration of 0.5, 1.0 and 2.0 mg/ml, respectively. The semen samples were incubated at 37oC for 15 …


Effect Of Supplementary Zinc From Organic And Inorganic Sources On Laying Performance And Zinc Deposition In Eggs, Hatairat Plaimast, Prapaisri Sirichakwal, Prapasri Puwastien, Suwanna Kijparkorn Sep 2008

Effect Of Supplementary Zinc From Organic And Inorganic Sources On Laying Performance And Zinc Deposition In Eggs, Hatairat Plaimast, Prapaisri Sirichakwal, Prapasri Puwastien, Suwanna Kijparkorn

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effect of supplementary organic and inorganic zinc on the laying performance and zinc deposition in eggs. One hundred and eighty laying hens (ISA Brown), aged 36 weeks, were randomly divided into 5 treatments with 4 replications of 9 hens each. A corn-soy based diet which was formulated to meet the recommended nutrient requirement especially the level of zinc (60 µµ µµ µg Zn/g diet) was used as the control diet. Both sources of zinc, inorganic (zinc sulfate) and organic (zinc amino acid chelate) were supplemented to provide zinc at levels of 300 and …