Veterinary Medicine Commons^™

Cystic Ovaries In Dairy Cattle, Lacey Morgan Rosenberg

Dairy Science

The objective of this literature review was to determine the potential causes and factors affecting the incidence of ovarian cysts in dairy cattle as well as to examine functionality of various preventative methods and treatments of cysts. The end product was to be a document outlining all the information available on the three different types of cysts that can develop on the bovine ovary, follicular cysts, luteal cysts, and cystic corpora lutea. The literature that was reviewed, most in the forms of research papers, articles, and reviews, came primarily from scientific journals and published books. The literature reviewed was only …

Characterization Of The Sero-Reactivity Of Proteins Map1152 And Map1156 From Mycobacterium Avium Subspecies Paratuberculosis, Avery L. Paulson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (JD) in ruminants. Development of genetic tools and completion of the MAP genome sequencing project expanded opportunities for antigen discovery. In this thesis, I review the current trends in diagnosis and disease control of JD and present the results of the studies on the seroreactivity of two proteins encoded for by the MAP1152-MAP1156 gene cluster. MAP1152 encodes for a PPE protein and MAP1156 encodes a diacylglycerol acyltransferase involved in triglyceride metabolism and classified in the uncharacterized protein family UPF0089. Maltose-binding protein (MBP) tagged recombinant MAP proteins were purified from Escherichia coli. …

Staphylococcus Aureus Virulence Factors Synthesis Is Controlled By Central Metabolism, Yefei Zhu

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments. This versatility depends on its ability to sense nutrients and respond by modulating gene expression, including the synthesis of virulence determinants. In addition to its ability to synthesize virulence factors, the capacity of S. aureus to form biofilms is an important mediator of virulence in certain infections. Biofilms are a complex aggregation of bacteria commonly encapsulated by an adhesive exopolysaccharide matrix (polysaccharide intercellular adhesin; PIA). To study S. aureus biofilm formation, we assessed the metabolic requirements of S. aureus growing in a biofilm and …

How I Became A Scientist: Activity Book For 3rd Graders, Jessica Schneider, Kauline Davis, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights how eight people became scientists from all over the world who work with animals. These scientists work hard to make sure that people and their animals stay healthy. Activities in the book cover topics related to each scientist's area of study.

Published in full color, the book features fun activities relevant to the different scientific specialties represented for children to complete. The publication of the book has been supported by the National Institutes of Health, the National Center for Research Resources, and a Scientific Education Partnership Award.

Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai

The Thai Journal of Veterinary Medicine

Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A …

Tetranucleotide Microsatellite Markers For Molecular Testing In Thai Domestic Elephants (Elephas Maximus Indicus), Duangsmorn Suwattana, Jutarat Jirasupphachok, Sumolya Kanchanapangka, Weerapong Koykul

The Thai Journal of Veterinary Medicine

Eleven microsatellite loci were evaluated for genetic profiles of Thai domestic elephants (Elephas maximus indicus) and their suitability as genetic markers for molecular testing. A total of 66 animals were tested. 10 out of 11 loci could be amplified and they demonstrated polymorphism with allelic numbers per locus ranging from 7 (LaT06) to 39 (LaT18). Values of expected heterozygosity (He) and Polymorphic Information Content (PIC) were between 0.6449 (LaT17) – 0.9593 (LaT05) and 0.5934 (LaT17) – 0.9578 (LaT05), respectively. Analysis of the ten microsatellite markers revealed Combined Exclusion Probability (CEP) of 99.99998783% or 1.2167 x 10-7 and 99.91% confident for …

An Outbreak Of Avibacterium Paragallinarum Serovar B In A Thai Layer Farm, Kridda Chukiatsiri, Suwit Chotinun, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Avibacterium paragallinarum serovar B was diagnosed and revealed in a layer farm. The infected chickens showed mucous nasal discharge, facial edema and conjunctivitis. Egg production also decreased dramatically. Necropsy findings showed lesions of conjunctivitis and edematous tissue around the eyes. The infraorbital sinuses were filled with a caseopurulent mass and tracheitis. Bacterial isolation and identification was performed and confirmed by PCR technic. A hemagglutination inhibition test using specific antibodies against serovar B revealed the Avibacterium paragallinarum serovar B infection. A pathogenicity test done in 10 layer chickens, showed clinical signs of nasal discharge, facial edema and conjunctivitis. This is the …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.

Effects Of Dietary Melamine Or Urea-Formaldehyde Or Their Mixtures On Performance, Carcass Quality, Melamine Residues And Microscopic Changes In Broiler Tissues, Srisuda Sirilaophaisan, Jowaman Khajarern, Bundit Tengjarernkul

The Thai Journal of Veterinary Medicine

A study was conducted to determine the effects of dietary graded levels of melamine, urea-formaldehyde (UF) and their mixture on growth performance, carcass quality, melamine residues and microscopic changes in broiler tissues from d-1 to d-42 followed by a 7-d feeding of withdrawal diets. One thousand and forty 1-d-old equal mixed-sex Arber Acres broiler were assigned to 13 dietary treatments with 4 replicate pens of 20 chicks assigned to each treatment. The diets of melamine, UF and their mixture contained four graded levels (0.25, 0.50, 0.75 and 1.00%) but no melamine or UF was added to the control diet. There …

In Vitro Testing Of The Efficacy Of Organic Releasing Chlorine (Virusnip™) Against Escherichia Coli, Salmonella Spp. Candida Albicans And Trichophyton Mentagophytes, Indhira Kramomtong, Waree Niyomtham, Sasiwimon Talummuk, Penchan Chaiyanate, Kai Sievert

The Thai Journal of Veterinary Medicine

The antimicrobial activities of organic releasing chlorine disinfectant (Virusnip™) was evaluated and compared by semi-quantitative suspension-neutralization method under clean condition in vitro. The test organisms were clinical bacterial isolates of hemolytic Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Hadar, Salmonella Infantis, and Salmonella Virchow. Fungal isolates were Candida albicans and Trichophyton mentagophytes. Four sets of Virusnip™ concentrations were prepared at 1:100, 1:200, 1:400 and 1:1000 for bacteria and 3 sets of 1:100, 1:200 and 1:400 for fungi. Each set of various concentrations was kept at room temperature for 1, 2 and 7 days before use. The assays were performed at …

Comparative Study Between Using Acetone And Absolute Alcohol For Dehydration In Plastination Procedure, Kongkiat Srisuwatanasagul, Sayamon Srisuwatanasagul, Adisorn Adirekthaworn, Damri Darawiroj

The Thai Journal of Veterinary Medicine

In order to develop the most practical plastination specimens used for anatomical study, the aim of the present study was to compare between using acetone and absolute alcohol for dehydration in plastination procedure. Ten pig heart specimens were used in the present study and plastination procedure was processed by using acetone (group 1) or absolute alcohol (group 2) for dehydration. The results showed that dehydration by acetone resulted in more natural color with no shrinkage of the heart specimens while dehydration by alcohol caused darker color and more shrinkage. However, the advantage of using alcohol was that it gave the …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Swine High Fever Syndrome: What Prrsv Gotta Do With It?, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

No abstract provided.

Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

K. parviflora is a medicinal plant possessing high potential for development of various health products. The objective of this chronic toxicity study was to investigate the safety of ethanolic extract of chronic Kaempferia parviflora Wall ex Bak in Wistar rats. The animals were randomly divided into five groups, twenty four rats each (12 males and 12 females). Three treatment groups were orally administered with K. parviflora extract at doses of 5, 50 and 500 mg/kg/day for six months respectively, which were equivalent to 1, 10 and 100 times of human use, while two control groups were orally given with distilled …

Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aim of the present study is to determine the distribution of immune cells within the endosalpinx related to the ovarian function in gilts culled due to repeat breeding and anoestrus. A total of 30 reproductive organs from culling crossbred Landrace x Yorkshire gilts were collected from commercial swine herds in Thailand and categorized into 3 groups, i.e. anoestrus, repeat breeding and non-involved in reproductive disorder gilts (control group). The ovaries and oviducts were separated from the reproductive tracts and macroscopically investigated. The oviducts were divided into the utero-tubal junction (UTJ), the isthmus, the ampulla and the infundibulum. The gilts …

Molecular Classification Of Infectious Laryngotracheitis Virus From Chick Embryo Origin Vaccine, Tissue Culture Origin Vaccine And Field Isolates, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The molecular classification of the infectious laryngotracheitis virus from chicken embryo origin (CEO) vaccine, tissue culture origin (TCO) vaccine and field isolates by the sequence of ICP4 genes from CEO and TCO vaccines in the GenBank was performed for the different sequence analysis. The sequences of TCO vaccine virus (accession no. EU104908) were used as a prototype for a primer design to cover the region of 450 basepairs (bp) by Primer3 software. The simulation sequences of CEO vaccine virus (accession no. EU104900) and TCO vaccine virus (accession no. EU104908) from PCR reaction were selected for restriction enzyme. Hga I could …

Salbutamol Residues In Swine Tissues And Body Fluids After Feeding, Jen Hung Ming, Hsiang Huang Han, Ling Chen Chao, Jen Wu Yu, Katie Marie Dixon, Liang Mao Chi

The Thai Journal of Veterinary Medicine

Salbutamol residues in swine tissues and fluids were investigated. Tissue, serum and urine samples were collected from 33 pigs after 14 days of oral administration of 3 ppm salbutamol. The concentrations of salbutamol were determined by enzyme-linked immunosorbent assay and further confirmed by capillary electrophoresis. The highest urinary concentration of salbutamol was 145.12 ng/ml at 18 hour and remained detectable for 30 days after the last feeding. Salbutamol was undetectable in serum samples. The highest concentrations of salbutamol in liver, kidney, lung, heart, brain and muscle were 70.42 ng/g, 31.88 ng/g, 26.06 ng/g, 6.76 ng/g, 3.41 ng/g and 2.97 ng/g, …

The Efficacy Of Pure Natural Repellents On Rat Responses Using Circular Open Field, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

Rodent infestation is considered as one of the major pest problems since it is not only acting as a health hazard, but also causes damages to household, agricultural fields and even transportation business. The use of rodenticides is a common approach to rodent control, however, most rodenticides are poisonous to man. Therefore, using natural extracts as a rat repellent may be a better alternative. In the current study, various natural extracts were experimented whether they could repel a rat when testing in the behavioral model, a circular open field. The tested substances were wintergreen oil, chilli, peppermint oil, bergamot oil …

Comparative Study Of Temperatures Used In Silicone Impregnation Of Porcine Hearts Plastination, Damri Darawiroj, Adisorn Adirekthaworn, Sayamon Srisuwattanasakul, Kongkiat Srisuwattanasakul

The Thai Journal of Veterinary Medicine

Forced impregnation process is normally performed under vacuum at freezing temperature which requires expensive plastination apparatus. To reduce the cost in the plastination process, forced impregnation of porcine hearts under two different environments, which are at the freezing temperature (-20oC) and at room temperature (22-25oC), was compared. Twelve porcine hearts were collected from a slaughter house, and divided into the groups, six in each, and subjected to be plastinated by S10 standard technique at the two temperatures. Although the forced impregnation at room temperature, also the curing process, was more time-consuming, all hearts from both groups produced satisfying plastinated specimens. …

Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Animal Health Matters, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- Olson Agricultural Analytical Services Laboratory Adapts to South Dakota Needs [Page] 2- Director's Message—Service and Grants [Page] 2- Veterinarian Named 2010 SDSU Distinguished Young Alumnus [Page] 3- Diagnostic Implications Following Use of a New IBR-PI3-BRSV Intranasal Vaccine [Page] 3- Extension Vet receives SDVMA Veterinarian of the Year Award [Page] 6- Survey of the Educational Needs of Animal Health Professionals in South Dakota, Summer 2010 [Page] 7- Certificate Program in Veterinary Preventive Medicine Offered Pieces and Parts [Page] 8- Internet Course Offerings from SDSU for Spring 2011 [Page] 8- VBS Department Faculty Members Update Chinese Delegation on Swine Diseases …

Importance Of Wildlife Disease Surveillance, Jerrold L. Belant, Amanda R. Deese

Human–Wildlife Interactions

A commentary on the importance of wildlife disease surveillance.

Growing South Dakota (Fall 2010), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Focused on Lean Growth: Despite Challenging Times, Dean Barry Dunn Has a Vision of Growth and Excellence for ABS
[Page] 6 Building Better Communities: Horizons Helps Small Town South Dakota
[Page] 8 On Campus
[Page] 9 Urban Opportunities: A New Twist to Attract Urban Youth
[Page] 10 Saddle Up: Steers for State Helps SDSU Rodeo Program Attain Success [Page] 12 Inspiring Students: This Professor's Teaching & Research Style Is Earning Awards and Student Accolades
[Page] 14 Fish Feed :The Newest Market for South Dakota Crops 15 College News [Page] 16 Field Notes from Lyman County: Bob Fanning's Experience …

Relationship Between Seminal And Serum Calcium Concentration With Semen Quality In The Asian Elephant (Elephas Maximus), Somsajee Sivilaikul, Amornrat Jitprom, Ajaree Kularb, Kornchai Kornkaewrut, Piyawan Suthanmaphinuth, Sittidet Mahasawangkul, Kulnasan Saikhun, Worawidh Wajjwalku, Sitthawee Thongtipsiridech

The Thai Journal of Veterinary Medicine

The purpose of this study is to identify the relationship of calcium concentration in seminal plasma on elephant semen quality including of volume, concentration, pH and percentage of progressive motility, dead sperm and abnormal morphology, respectively. Semen collection and evaluation were done in 9 elephants of Thai Elephant Conservation Centre, National Elephant Institute, Forest Industry Organization at Lampang. Calcium in seminal plasma was measured by using colorimetric method. Data were analyzed by using Linear Regression. The results revealed that amount of calcium in seminal plasma was negative correlated with only percentage of progressive motility (p<0.05). Ejaculates were separated into three groups based on their progressive motility percentage including of low, moderate and high-motile semen (0-5%, >5-40% and >50%, respectively) and …

Chronic Mastitis In Small Dairy Cattle Herds In Muang Khon Kaen, Jaruwan Kampa, Varaporn Sukolapong, Anantachai Chaiyotwittakun, Sarinya Rerk-U-Suke, Arunee Polpakdee

The Thai Journal of Veterinary Medicine

Monthly records of bulk tank milk somatic cell count (BMSCC) of all 194 dairy herds in Muang, Khon Kaen were used as an indicator of chronic mastitis problems; i.e. BMSCC > 500,000 cells/ml at least 3 consecutive records. Twelve chronic mastitis herds in Muang, Khon Kaen were data collected on 1) general herd information, 2) milking equipment, 3) milking procedure and 4) awareness and management of farmers to the mastitis problem. All possible mastitis pathogens were investigated in individual milk samples from 138 lactating cows of the herds. At least 40% of dairy herds in Muang, Khon Kaen had mastitis at …

Analysis On The Polymorphism And The Genetic Effects On Some Economic Traits Of Mx Gene S631n Mutation Site In Chicken, Luan De Qin, Bin Chang Guo, Wei Sheng Zhong, Liu Yan, Hong Chen Guo

The Thai Journal of Veterinary Medicine

PCR-RFLP technique was performed to detect the distribution differences of the resistant and sensitive alleles of S631N site of Mx gene in 8 chicken populations. The results showed the average frequencies of resistant allele A and sensitive allele G were 0.4995 and 0.5005, respectively, and the average value of observed heterozygosis and Shannon’s index were 0.6163 and 0.5232. Three populations were in Hardy-Weinberg equilibrium at this site (p>0.05). The Ewens-Watterson test indicated that this site was neutral in all the other populations except for Rugao. The dendrograms based on allele frequency divided the 8 populations into 3 clusters, which …

The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. …

Blood Pressure, Urinary Protein Creatinine Ratio And Oxidative Stress In Dogs With Urolithiasis, Supa Sithanukul, Poramin Shayarattanasin, Vimolpa Hiranpradith, Winai Chansaisakorn, Monkon Trisiriroj, Siripen Komolvanich, Saikaew Satayatham, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

Blood pressure, urinary protein creatinine (UPC) ratio and oxidative stress (plasma melondialdehyde, urinary melondialdehyde creatinine ratio; UMDA/Cr, the activities of red blood cell catalase; RBC-CAT and red blood cell glutathione) were studied in 42 dogs with urolithiasis and 14 control healthy dogs. The types and location of calculi were defined. The results showed that calculi preferentially found in male and female dogs were calcium oxalate and struvite, respectively. In female, calculi found mostly at urinary bladder while in male, they could be found in either urinary bladder and/or urethra. Dogs with urolithiasis were normotensive. However, UPC ratio was higher significantly …

Evaluation Of Serum Nt-Probnp Levels In Dogs With Respiratory Distress Associated With Heart Disease And Respiratory Disease, Amornrate Sastravaha, Santi Kaewmokul, Pareeya Udomkusonsri, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

The association between severity of heart disease and serum N-terminal prohormone B-type natriuretic peptide (NT-proBNP) was determined in dogs presented with respiratory distress. Four groups of dogs were divided into healthy control (n=11), heart failure (n=58), heart disease without heart failure (n=17) and respiratory disease (n=20). Serum NT-proBNP concentrations were measured with an ELISA designed for using in dogs. Dirofilaria antigen tests were detected in dogs with heart disease or heart failure. Serum NT-proBNP concentrations of dogs with heart failure (2,977±184 pmol/l) were significantly higher than dogs with heart disease (611±46 pmol/l), respiratory disease (583±52 pmol/l) or physically healthy (426±32 …