Veterinary Medicine Commons^™

Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in …

Ultrasound-Guided Transvaginal Follicular Aspiration And Development Of Vitrified-Thawed Thai Indigenous Beef Cattle (Bos Indicus) Oocytes After In Vitro Fertilization, Denpong Sakhong, Thevin Vongpralub, Suporn Katawatin, Saksiri Sirisathien

The Thai Journal of Veterinary Medicine

This study was done to evaluate the efficiency of ultrasound-guided transvaginal follicular aspiration without hormonal pre-stimulation for collecting the oocytes of indigenous Thai beef cattle (Bos indicus), and to assess the vitrification method for their preservation. All animals between 2.5-12 years were categorized according to age (i.e. heifer, young cow, and old cow). A medium-sized follicle (φ 3-8 mm.) had the greatest distribution among the various size classes (p≤ 0.05), and the mean of oocytes collected from each group was not significantly different. Postthawed survival was 60.78, 53.70 and 57.50%, with a cleavage rate of 38.71, 37.93 and 39.13%, and …

Report Of Lawsonia Intracellularis Infection In Dogs By Polymerase Chain Reaction, Sariya Asawakar, Suphot Watanaphansak, Tanong Asawakarn

The Thai Journal of Veterinary Medicine

The purpose of this study was to survey Lawsonia intracellularis in dogs in Thailand by using Polymerase chain reaction technique. Lawsonia intracellularis is obligatory intracellular bacterium which causes proliferative enteropathy in animals, including dog. Fecal samples from 48 dogs were collected from Bangkok, Pathumthani and Nakhon Ratchasima and tested for L. intracellularis by PCR technique. The dogs were classified into two groups according to the presence or absence of diarrhea. In the group of dogs with diarrhea (n= 11), 6 dogs were positive (54.4%) and in the group without signs of diarrhea (n= 37), 15 dogs were positive (40.5%). The …

Histology Of Extrahepatic Bile Ducts In The Iranian Chukar Partridge (Alectoris Chukar), Behzad Mobini, Kaveh Shirani Faradonbeh

The Thai Journal of Veterinary Medicine

A study on the histology of the extrahepatic bile ducts was conducted in eight female and eight male twentyweek- old healthy Iranian partridges (Alectoris chukar). The extrahepatic bile ducts possessed tunica mucosa, muscularis and serosa. The tunica mucosa of the extrahepatic bile ducts was lined by non ciliated simple columnar epithelium. The apical cytoplasm of epithelial cells was covered by a continuous striated border of microvilli. The goblet cells were absent in epithelium. Tunica mucosa formed some simple folds lining with tall columnar epithelium. The lamina muscularis mucosa was absent. All epithelial cells of the bile ducts reacted with acid …

Ophthalmology Snapshot, Tuntivanich Nalinee

The Thai Journal of Veterinary Medicine

No abstract provided.

Pengujian In Vitro Gelatin Sagu, Sumber Npn, Mineral Kobalt Dan Seng Pada Cairan Rumen Domba (Evaluation In Vitro Gelatinized Sago, Sources Npn, Minerals Cobalt And Zinc On The System Of Sheep Rumen), Harry Triely Uhi

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian in vitro bertujuan mengamati pengaruh perlakuan gelatin sagu, amonium sulfat, mineral seng dan kobalt. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan 9 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalah R1  (gelatin sagu + urea  + Co 0.2 ppm); R2  (gelatin sagu + urea  + Zn 35 ppm); R3  (gelatin sagu + urea + Co 0.2 ppm dan Zn 35 ppm); R4  (gelatin sagu + amonium sulfat + Co 0.2 ppm); R5  (gelatin sagu + amonium sulfat + Zn 35 ppm); R6  (gelatin sagu + amonium sulfat + Co 0.2 ppm dan Zn 35 ppm); R7  (gelatin …

Fermentabilitas Dan Kecernaan Ransum Lengkap Sapi Perah Berbasis Jerami Padi Dan Pucuk Tebu Teramoniasi (In Vitro) Fermentability And Digestibility Of Rice Straw And Cane Top Ammoniated Based Complete Rations (In Vitro), U. Hidayat Tanuwiria

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan mempelajari fermentabilitas dan kecernaan ransum lengkap sapi perah berbasis jerami padi dan pucuk tebu teramoniasi in vitro. Penelitian dilakukan secara eksperimen dengan rancangan acak lengkap (RAL) enam perlakuan yang diulang lima kali. Perlakuan terdiri atas R1 (60% RG + 40% konsentrat), R2 (30% RG + 30% JPA + 40% konsentrat), R3 (30% RG + 30% PTA + 40% konsentrat), R4 (30% JPA + 30% PTA + 40% konsentrat), R5 (60% JPA + 40% konsentrat) dan R6 (60% PTA + 40% konsentrat). Hasil penelitian menunjukkan bahwa Fermentabilitas pucuk tebu diamoniasi lebih tinggi daripada jerami padi diamoniasi, hal ini …

Growing South Dakota (Fall 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Kudos To South Dakota Farmers: SDSU Study Credits Farmers For Conserving Soil, Sequestering More Carbon
[Page] 4 Fall College News
[Page] 6 Investing In Research: Additional Appropriations Being Sought For SDSU’s Ag Station Research
[Page] 8 Pertinent Publications: Plant Science Department Produces Several New Guide Books
[Page] 9 Special Pull-Out Section: Snapshot from SDSU’s College of Agriculture & Biological Sciences: Highlighting Academic Programs, Ag Experiment Station and SDSU Extension
[Page] 13 Plans Proceed: Efforts For Cow/Calf Training And Research Center Continue
[Page] 14 Outreach Through Horses: Initiative Teaches Equine Care On South Dakota Reservations
[Page] 16 Here And …

Mycoplasma Haemocanis – The Canine Hemoplasma And Its Feline Counterpart In The Genomic Era, Naila C. Do Nascimento, Andrea P. Santos, Ana Ms Guimaraes, Phillip J. Sanmiguesl, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma haemocanis is a hemotrophic mycoplasma (hemoplasma), blood pathogen that may cause acute disease in immunosuppressed or splenectomized dogs. The genome of the strain Illinois, isolated from blood of a naturally infected dog, has been entirely sequenced and annotated to gain a better understanding of the biology of M. haemocanis. Its single circular chromosome has 919 992 bp and a low G + C content (35%), representing a typical mycoplasmal genome. A gene-by-gene comparison against its feline counterpart, M. haemofelis, reveals a very similar composition and architecture with most of the genes having conserved synteny extending over their entire chromosomes …

A Toxicology And Clinical Study Of Post Race Epitaxis Associated With Exercise Induced Pulmonary Hemorrhage In Thoroughbred Race Horses At The Racecourse Rinconada, Caracas, Venezuela, Abelardo Morales Briceno, Diana Villoria Leon, Kimberly Brewer, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

The aim of this study was to describe cases of epistaxis associated with post-race Exercise Induced Pulmonary Hemorrhage (EIPH) in Thoroughbred horses at the Hippodrome "La Rinconada", Caracas, Venezuela, through a clinic pathological study. We studied a total of 29 cases of epistaxis post-race in Thoroughbred horses at the Hippodrome La Rinconada, Caracas, Venezuela, which is 2,950 meters above sea level. The study included horses between the ages of 2-5 years, 16 stallions and 13 mares, weighing between 450-510 kg. They underwent a clinical examination, although horses presenting with epistaxis were in an emergency situation. Samples of blood and urine …

The Effect Of Infection Order Of Porcine Circovirus Type 2 And Porcine Reproductive And Respiratory Syndrome Virus On Dually Infected Swine Alveolar Macrophages, Yi-Chieh Tsai, Hui-Wen Chang, Chian-Ren Jeng, Tsang L. Lin, Chun-Ming Lin, Cho-Hua Wan, Victor Fei Pang

Department of Comparative Pathobiology Faculty Publications

Abstract Background: Concurrent infection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is known as one of the major causes for porcine respiratory disease complex (PRDC). Dual infection with PCV2 and PRRSV is consistently to have more severe clinical presentations and pulmonary lesions than infection with PCV2 alone or PRRSV alone. However, it is not known if dual infections with PCV2 and PRRSV in different infection order may lead to different clinical symptoms in the host. To mimic the possible field conditions, swine alveolar macrophages (AMs) were inoculated with PCV2 and PRRSV in vitro …

Ultraviolet Radiation–Induced Cataract In Mice: The Effect Of Age And The Potential Biochemical Mechanism, Jie Zhang, Hong Yan, Stefan Lofgren, Xiaoli Tian, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. To study the effect of age on the morphologic and biochemical alterations induced by in vivo exposure of ultraviolet radiation (UV).

METHODS. Young and old C57BL/6 mice were exposed to broadband UVBþUVA and euthanized after 2 days. Another batch of UV-exposed young mice was monitored for changes after 1, 2, 4, and 8 days. Age-matched nonexposed mice served as controls. Lens changes were documented in vivo by slit-lamp biomicroscopy and dark field microscopy photographs ex vivo. Lens homogenates were analyzed for glutathione (GSH) level, and the activities of thioredoxin (Trx), thioltransferase (TTase), and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Glutathionylated lens proteins …

Investigation Of The Role Of Tnf-A Converting Enzyme (Tace) In The Inhibition Of Cell Surface And Soluble Tnf-A Production By Acute Ethanol Exposure., Kristine Von Maltzan, Wei Tan, Stephen B. Pruett

CVM Publications

Toll-like receptors (TLRs) play a fundamental role in the immune system by detecting pathogen associated molecular patterns (PAMPs) to sense host infection. Ethanol at doses relevant for humans inhibits the pathogen induced cytokine response mediated through TLRs. The current study was designed to investigate the mechanisms of this effect by determining whether ethanol inhibits TLR3 and TLR4 mediated TNF-? secretion through inhibition of transcription factor activation or post-transcriptional effects. In NF-?B reporter mice, activation of NF-?B in vivo by LPS was inhibited by ethanol (LPS alone yielded 170,000?35,300 arbitrary units of light emission; LPS plus ethanol yielded 56,120?16880, p = …

Variation In Genes Related To Cochlear Biology Is Strongly Associated With Adult-Onset Deafness In Border Collies, Jennifer S. Yokoyama, Ernst T. Lam, Alison L. Ruhe, Carolyn A. Erdman, Kathryn R. Robertson, Aubrey A. Webb, D. Colette Williams, Melanie L. Chang, Marjo K. Hytönen, Hannes Lohi, Steven P. Hamilton, Mark W. Neff

Anthropology Faculty Publications and Presentations

The domestic dog offers a unique opportunity to study complex disorders similar to those seen in humans, but within the context of the much simpler genetic backgrounds of pure breeds, which represent closed populations. We performed a whole-genome search for genetic risk factors of adult-onset deafness in the Border Collie, a breed of herding dog that relies on acute hearing to perceive and respond to commands while working. Adult-onset deafness in Border Collies typically begins in early adulthood and is similar to age-related hearing loss in humans. This earlier onset has particular impact on the utility of working Border Collies …

Canine Urinary Incontinence Post-Neutering: A Review Of Associated Factors, Pathophysiology And Treatment Options, Suppawiwat Ponglowhapan, Muhammad Khalid, David Church

The Thai Journal of Veterinary Medicine

Canine urinary incontinence is commonly encountered in small animal veterinary practice with highprevalence in spayed female dogs. The diagnosis of neutering-induced urinary incontinence is usually based onclinical signs, history and elimination of other possible diagnoses. The proposed predispositions to incontinence thatfollows neutering including gender, breed, body weight, obesity, tail docking, spaying technique (ovariectomy andovariohysterectomy) and morphology of the LUT, e.g. the position of neck of the bladder and urethral length, have allbeen investigated. At present, the exact underlying mechanisms are not fully understood. However, it is widelyagreed that the condition is multi-factorial and hormone-associated. The role of gonadotrophin LH and …

Preliminary Study Of The Clinical Outcome Of Using Pcso-524 Polyunsaturated Fatty Acid Compound In The Treatment Of Canine Osteoarthritis And Degenerative Spinal Diseases, Natwadee Mongkon, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

The clinical outcome of PCSO-524 application in the treatment of canine osteoarthritis and spinal disorderwas studied at the Small Animal Teaching Hospital, Chulalongkorn University. Eighty-four dogs were included inour study. Thirty-one dogs showed signs of osteoarthritis (OA) in both hip joints and shoulder joints; 33 dogs had OAin stifle joints from cranial cruciate ligament rupture; and 20 dogs had neurological signs of the cauda equinasyndrome. All affected dogs were treated by 50 mg/ 10 kg of PCSO-524, PO for twelve weeks. Neurological grading,lameness scores and neurological examinations were evaluated before and after the administration of PCSO-524 fortwo and four weeks. …

Experimental Infection Of Mice And Baby Chickens With Thailand Strain Of Chikungunya Virus, Sonthaya Tiawsirisup, Pichanan Rattanakampol, Warritar Navavichit, Hemmarat Ratpiyapaporn

The Thai Journal of Veterinary Medicine

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven …

Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aims of the present study were to determine the number of spermatozoa in the reproductive tract ofsows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence ofadding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteenmultiparous sows were divided into 3 groups: group I (n= 5), insemination with 80 ml of extended fresh semen,group II (n= 4), insemination with 40 ml of FT semen diluted with ModenaTM extender and group III (n= 5),insemination with 40 ml of FT semen diluted with combination of ModenaTM and seminal plasma. …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Hand, Foot, And Mouth Disease, Juthatip Keawcharoen

The Thai Journal of Veterinary Medicine

No abstract provided.

Anxiolytic-Like Effects Of Noni Juice (Morinda Citrifolia L.) On The Respective Changes Of Neurotransmitters In Rat Brain In The Elevated Plus-Maze Test, Sarinee Kalandakanond-Thongsong, Jantarima Charoenphandhu

The Thai Journal of Veterinary Medicine

Noni (Morinda citrifolia L.) has long been used to treat various conditions in folklore medicine. Presently,most studies focus on antioxidant and anti-cancer activities while it is also claimed by noni juice-consumers toproduce happiness. Mood disorders, e.g. anxiety, are symptoms that reflected the disorder of neurotransmitters, oneof the most prominent is the monoaminergic system. Anxiety affects people throughout the world; people have beenseeking medicine for treatment while natural products may also alleviate this unfavorable symptom. The presentstudy examined whether noni contained anxiolytic-like effect when tested with elevated plus-maze (EPM), a standardtest for anxiety, and whether this effect was related to change …

Side Effects In 412 Dogs From Swimming In A Chlorinated Swimming Pool, Korakot Nganvongpanit, Terdsak Yano

The Thai Journal of Veterinary Medicine

This study reports the side effects in dogs from swimming in a chlorinated swimming pool. Data werecollected from September 2008 until April 2012. A total of 412 dogs (male= 219 and female= 193), aged 38±30 months,served as the subjects. The dogs were divided into four groups based on the aims of swimming: entertainment (n=152), weight reduction (n= 53), rehabilitation after orthopedic surgery (n= 81), and rehabilitation for muscle and jointdisease (n= 126). The data were recorded for five separate swimming times. At first, some dogs (29.13%) showedoverexcitement and/or fear, but this percentage decreased with increased swimming frequency. Some dogs (36.51%)were …

An Anatomical, Histological And Ultrastructural Study On The Placenta Of The Red-Shanked Douc Langur (Pygathrix Nemaeus), Damri Darawiroj, Natthaya Tuaprakone, Yongchai Utara

The Thai Journal of Veterinary Medicine

A red-shanked douc langur (Pygathrix nemaeus nemaeus) is currently enlisted as an endangered primatespecies. This study was undertaken to describe the gross anatomy, histology and ultrastructural morphology of thedouc langur term placenta by light, scanning, and transmission electron microscopy. The primary disc of bidiscoidalplacenta was separated to 4-6 small cotyledonary subdivisions and was attached by an umbilical cord. The chorionfrondosum of placenta was classified as villous, haemomonochorial and deciduate type. The interhaemal area wascomposed of a layer of syncytiotrophoblast that occasionally clustered as the syncytial knot, fetal capillaryendothelium and its basement membrane. The intervillous mineralized fibrinoid mass and syncytial knot …

Detection Of Enzootic Bovine Leukosis In Cattle Using Nested Polymerase Chain Reaction Assay, Jomelson Alfaro Uera, Jonathan Ventura Lazaro, Claro Niegos Mingala

The Thai Journal of Veterinary Medicine

Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV) infection. BLV was detected incattle using nested polymerase chain reaction (PCR) assay and were identified BLV infected cattle farms in fiveselected provinces in the Philippines. A total of 300 cattle blood samples were used. BLV Proviral DNA wasextracted and amplified using nested PCR assay targeting the BLV long terminal repeat (LTR). Results showed that11 samples (3.67%) of the 300 cattle blood samples used were positive for BLV infection. This study is considered firstreport of cattle EBL in the Philippines.

Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki

The Thai Journal of Veterinary Medicine

We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …

Surgical Treatment Of Renal Gout In Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome

The Thai Journal of Veterinary Medicine

A wild captured, male Monocellate cobra, Naja kaouthia of unknown age was observed with anorexia, imbalance of swimming and mild swelling in the distal fourth of the body. Physical examination revealed a palpable firm mass in the coelomic cavity, of which radiographic examination was suspicious as an obstruction of large intestine by a foreign body. Removal of the foreign body through its cloaca was attempted by enema and digital manipulation, but was not successful. Thereafter, an exploratory surgery was performed and revealed an abnormality in the left kidney that was compressing the large intestine. Nephrectomy was performed and histopathologically identified …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Influence Of Intracellular Glutamine Depletion On Regulation Of Amino Acid Transport System A In Placental (Bewo) Cells, Boonrit Thongsong

The Thai Journal of Veterinary Medicine

Glutamine is known to modulate the expression of various genes and uptake activity of nutrient transportersin mammalian cells. The purpose of the study was to investigate the influence of intracellular glutamine status onamino acid transport system A in placental choriocarcinoma cells (BeWo). Intracellular glutamine was depleted byculturing the cells in regular medium without glutamine and by treating the cells for 16 hours with methioninesulfoximine (MSX), an inhibitor of glutamine synthetase. When cultured in the presence of glutamine, treatment withMSX had no effect on system A activity as monitored by MeAIB uptake. However, when cultured in the absence ofglutamine, treatment with …

Differential Expression Patterns Of Proteins Involved In Epidermal Proliferation And Differentiation In Canine Atopic Dermatitis, Sirin Theerawatanasirikul, Achariya Sailasuta, Roongroje Thanawongnuwech, Tossaporn Nakbed, Komkrid Charngkaew, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis(CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesionalCAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotichyperkeratosis. Immunohistochemistry (IHC) of Ki-67, a cellular marker for proliferation, showed a higher epidermalcell proliferation rate in the CAD dogs (p< 0.05) which positively correlated with Canine Atopic Dermatitis Extent andSeverity Index, CADESI-03 (p< 0.01). IHC labeling indicated reduced expression of cornified envelope proteins,involucrin (IVL) and filaggrin (FLG) in both lesional and non-lesional skin (p< 0.05). In contrast, expression oflympho-epithelial Kazal-type inhibitor (LEKTI), which has been reported to inhibit proteases that cleave proFLG intoFLG, was increased in lesional skin compared to the normal controls (p< 0.05). The IHC results showed a positivecorrelation between CADESI-03 and LEKTI expression and a negative correlation between CADESI-03 and IVLexpression. In conclusion, the present study demonstrates that epidermal hyperkeratosis in CAD is related todisturbance of both epidermal proliferation and cornified envelope differentiation. This is the first report of acorrelation of LEKTI with CAD.