Veterinary Medicine Commons^™

Toxicity Test Of Nanosilver Particles On Zebrafish (Danio Rerio) Embryonic Development, Theerayuth Kaewamatawong, Aranya Ponpornpisit, Wijit Banlunara, Anong Bintvihok, Benchawan Tosukcharoen, Siriwipa Kongloon, Suthasinee Udchachon, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit

The Thai Journal of Veterinary Medicine

Contamination of the environment with Nanosilver particles (AgNPs) has caused considerable concern. Atpresent there is little information of AgNPs toxicity, especially in aquatic environments. The purpose of this studywas to evaluate toxic effects of AgNPs on Zebrafish (Danio rerio) embryonic development. Toxicity test of AgNPs onthe embryos was conducted in concentration of 0.05, 0.1, 0.5, 1 and 5 μg/ml. Standard water was used as control.Observation for mortality, morphological and functional abnormalities at 4, 24, 48 and 72 hours post fertilization (hpf)were performed. The results showed that AgNPs at 1 and 5 μg/ml had significant lethal effect on the embryos.Calculated LC50 …

Stimulation Patterns For Erection And Ejaculation Using Electroejaculator In Black Marsh Turtles, Sakdichod Kimskulvech, Peerasak Suttiyotin

The Thai Journal of Veterinary Medicine

Semen collection by an electroejaculator was studied in black marsh turtles. High responses were recordedwhen electrical probe was inserted to the depth of 5.9±0.1 cm from the anus or 5.2±0.2 cm from posterior tip of thecarapace. The depth position was approximately the center of the second carapace from the rear. The intensity ofresponses increased with increasing voltage and reached a plateau at 7.0 volts. The depth of the probe inserted toyield highest responses had higher relationship with carapace length when measured against the anus (r= 0.81, p=0.0088) than the posterior end of carapace (r= 0.06, p= 0.8825). Stimulation voltages have …

Safety Evaluations Of Ethanolic Extract Of Moringa Oleifera Lam. Seed In Experimental Animals, Songpol Chivapat, Pornchai Sincharoenpokai, Praw Suppajariyawat, Anudep Rungsipipat, Suphan Phattarapornchaiwat, Vanida Chantarateptawan

The Thai Journal of Veterinary Medicine

The safety of ethanolic extract from the seeds of Moringa oleifera was evaluated for its oral toxicity by acute toxicity test and subchronic toxicity study in experimental animals. The acute toxicity test in mice showed that the extract at doses ranging from 5.1 to 10.0 g/kg caused toxic signs and dose dependent mortality. Oral administration of the extract in rats at doses of 100, 500 and 1000 mg/kg/day for 90 consecutive days revealed that male rats treated with the extract at 1000 mg/kg had a significant decrease in RBC but this alteration was within rats reference range. A significant decrease …

Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra

The Thai Journal of Veterinary Medicine

This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …

Apitoxin Aquapuncture And Administration Of Herbal Medicines In A Dog With Superficial Pyoderma, Cheng-Hung Lai, Pan-Chen Liu, Han-Yuan Lin

The Thai Journal of Veterinary Medicine

A 6-month-old, 7.2 kg, intact female mongrel stray dog wasreferred to the Veterinary Medical TeachingHospital, National Chung Hsing University with a chief complaint of alopecia, and severe pruritus. A skin swab wasobtained for bacterial culture, and Staphylococcus pseudintermedius was identified. The definitive diagnosis was caninesuperficial pyoderma. Apitoxin aquapuncture was performed to treat superficial pyoderma once a week for 5 weeks.The acupoints used in this clinical case included GV-14 (Da Zhui), BL-13 (Fei Shu), LI-11 (Qu Chi), BL-40 (WeiZhong), ST-36 (Zu San Li), SP-6 (San Yin Jiao), and SP-10 (Xue Hai). In addition, the herbal medicines Shi Wei Bai DuTang, Huang …

Preservation Of Sudan Red Staining Of Brain After Plastination Process, Punnarat Vibulchan, Jantima Intarapanya, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

Specimen preservation is an essential factor in gross anatomical study of the brain and spinal cord. Forcedimpregnation of the specimen using polymer, or plastination, is to date one of the best specimen preparationprocedures. This study aimed to study the possibility of staining whole brain specimen using Sudan Red III dye priorto plastination. Dog brains were divided into 3 groups each stained with the dye for 1, 2 and 3 months, respectively.The stained specimens were then dissected to reveal the staining of deep structures before undergoing theplastination procedure. After the experiment we found that Sudan Red clearly stained the fiber tracts …

Combination Of Acupuncture And Aquapuncture Using Vitamin B Complex For Treatment Of Chronic Degenerative Changes Of Hip Joints And Anal Relaxation In A Dog, Kui-Ming Chang, Kuan-Sheng Chen, Hsien-Chi Wang, Cheng-Hung Lai, Yu-Ta Hsieh, Hsing-Hui King, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A beagle dog, aged 10 years old, was presented with anal relaxation. Chronic arthrosis of hip joints had beendiagnosed and treated on this dog’s first visit to the National Chung Hsing University Veterinary Medicine Teachinghospital. Except for unwillingness to walk on both hind limbs, pain on both hips and uncontrolled defecation due toanal relaxation, no other clinical signs were found during physical examination. The body temperature was 38.60C.CRT was normal (less than 2 sec). Heart beat was 110 beats/min and strong pulse was palpated. Based on theGlasgow composite pain scale, the pain scale of this dog was 2. Radiology of …

Ecg Quiz, Chollada Buranakarl, Suwanakiat Sawangkoon, Monkon Trisiriroj, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander

Department of Animal Science: Faculty Publications

Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left–right alternation of limbs as well as coordinated activation of flexor and extensor muscles. Here we show that a premature stop codon in the DMRT3 gene has a major effect on the pattern of locomotion in horses. The mutation is permissive for the ability to perform alternate gaits and has a favorable effect on harness racing performance. Examination of wild-type and Dmrt3-null mice demonstrates that Dmrt3 is expressed in the dI6 subdivision of spinal cord neurons, takes …

Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong

Master's Theses

Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …

Let's Cure Cancer!, Wallace Morrison, Jessica Schneider, Sandra F. Amass

College of Veterinary Medicine eBooks

From ancient Egypt to modern times, scientists have been learning new ways to prevent, diagnose, and treat cancer. In this book, you’ll learn about cancer and how veterinarians, veterinary technicians, physicians, and other scientists are working together to find a cure for cancer in people and their pets.

Fat Dogs And Coughing Horses: Be The Vet! Solve The Case!, Kauline Cipriani Davis, Jessica Schneider, Henry Green Iii, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights various careers in the veterinary profession and provides veterinary medical cases for the reader to solve.

This project was supported by the National Center for Research Resources and the Division of Program Coordination, Planning, and Strategic Initiatives of the National Institutes of Health through Grant Number R25 OD011115-04.

Also attached are the 6 fitness handouts - Canine Obesity, Dog Weight by Breed, Doggie Fitness, Eating Healthy, Healthy Treats, and Weather Tips.

How I Became A Veterinarian, Kauline Cipriani Davis, Sandra F. Amass, Paula Green

College of Veterinary Medicine eBooks

Veterinarians have fun and exciting jobs! In this book you will meet veterinarians from all over the world. These veterinarians work hard to make sure people and their animals stay healthy. Maybe one day you will be a veterinarian too!

Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates, L. M. Nemec, J. D. Richards, C. A. Atwell, D. E. Diaz, G. I. Zanton, T. F. Gressley

Physiology Collection

The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). …

Expression And Distribution Of Thiol-Regulating Enzyme, Glutaredoxin 2 In Porcine Ocular Tissues, Bijaya Prasad Upadhyaya

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Glutaredoxin 2 (Grx2), a thiol-regulating enzyme of oxidoreductase family and a mitochondrial isozyme of glutaredoxin 1, was discovered 11 years ago in our laboratory. Grx2 is present in the lens where it shows dethiolase, peroxidase, and ascorbate recycling activities. Recently, Grx2 has also been identified to protect the mitochondrial electron transport system with anti-apoptotic function. Since other eye tissues besides the lens are rich in mitochondria and are very sensitive to oxidative stress, we speculate the presence of Grx2 therein as an important redox regulator. This study is to investigate the expression and distribution of Grx2 in ocular tissues using …

Melanopsin Mediates Retrograde Visual Signaling In The Retina, Dao-Qi Zhang, Michael A. Belenky, Patricia J. Sollars, Gary E. Pickard, Douglas G. Mcmahon

School of Veterinary and Biomedical Sciences: Faculty Publications

The canonical flow of visual signals proceeds from outer to inner retina (photoreceptors→bipolar cells→ganglion cells). However, melanopsin-expressing ganglion cells are photosensitive and functional sustained light signaling to retinal dopaminergic interneurons persists in the absence of rods and cones. Here we show that the sustained-type light response of retinal dopamine neurons requires melanopsin and that the response is mediated by AMPA-type glutamate receptors, defining a retrograde retinal visual signaling pathway that fully reverses the usual flow of light signals in retinal circuits.

Development Of One-Step Taqman® Real-Time Reverse Transcription-Pcr And Conventional Reverse Transcription-Pcr Assays For The Detection Of Equine Rhinitis A And B Viruses, Zhengchun Lu, Peter J. Timoney, Jena White, Udeni B R Balasuriya

Veterinary Science Faculty Publications

BACKGROUND: Equine rhinitis viruses A and B (ERAV and ERBV) are common equine respiratory viruses belonging to the family Picornaviridae. Sero-surveillance studies have shown that these two viral infections are prevalent in many countries. Currently, the diagnosis of ERAV and ERBV infections in horses is mainly based on virus isolation (VI). However, the sensitivity of VI testing varies between laboratories due to inefficient viral growth in cell culture and lack of cytopathic effect. Therefore, the objective of this study was to develop molecular diagnostic assays (real-time RT-PCR [rRT-PCR] and conventional RT-PCR [cRT-PCR] assays) to detect and distinguish ERAV from ERBV …

Animal Health Matters, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- SDVMA and SDSU Initiate Stethoscope Ceremopny for Outgoing Pre-Veterinary Students [Page] 2- Director's Message: Planting Seeds for the Next Generation of Veterinarians in South Dakota [Page] 2- Dr. David Francis Honored at Retirement Ceremony [Page] 3- 2012-2013 SDSU Veterinary and Biomedical Sciences Department Scholarship Award Winners [Page] 4- Accounting and Human Resources Staff Keep the VBS Department Running [Page] 5- Pre-Veterinary Club Sparks SDSU One Health Coalition; Hosts One Health Commission CEO Pieces and Parts [Page] 5- Faculty Members Learn of Admission Changes at Iowa State University Regional Veterinary Education Conference [Page] 6- South Dakota Honors Veterinary and …

Growing South Dakota (Summer 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 150 Years and Counting: 2012 Marks a Significant Milestone for Land-Grant Universities [Page] 4 Summer College News
[Page] 6 Ready, Set, Grow: Gardening Programs Offer bevy of Benefits to Community
[Page] 8 SDSU Extension Staff Directory: Pull Out and Save for Future Reference
[Page] 13 Bugs Beware!: SDSU Assembles Talented Team of Entomologists
[Page] 14 High-Tech Teaching: Curriculums Now Integrate iPads, Social Media & Peer Mentoring
[Page] 16 A Lesson in Chinese: SDSU Contingent Experiences World's Largest Marketplace
[Page] 18 Here and There
[Page] 20 Adapting with Apps: Technological Advancements Offer Opportunities for Farming's Future
[Page] 21 A …

A Century And A Half Of Research On The Stable Fly, Stomoxys Calcitrans (L.) (Diptera: Muscidae), 1862-2011: An Annotated Bibliography, K. M. Kneeland, Steven R. Skoda, Jerome A. Hogsette, A. Y. Li, Jaime Molina-Ochoa, Kimberly Hutchinson Lohmeyer, John E. Foster

Department of Entomology: Faculty Publications

The stable fly, Stomoxys calcitrans, is a cosmopolitan pest of livestock, wild animals, pets, and humans. It is a primary pest of cattle in the United States, estimated to cause more than $1 billion in economic losses annually. It also causes dissension at the rural-urban interface and is a problem in recreation areas such as Florida beaches and the Great Lakes. Due to its pestiferous nature and painful bite, methods to control stable flies have been investigated for over a century. A large amount of research has been reported on stable fly biology, ecology, genetics, physiology, and vector competence. …

Risk Factors Of Subclinical Mastitis In Small Holder Dairy Cows In Khon Kaen Province, Chaiwat Jarassaeng, Suneerat Aiumlamai, Chalong Wachirapakorn, Mongkol Techakumphu, Jos P.T.M. Noordhuizen, Anton C. Beynen, Siriwat Suadsong

The Thai Journal of Veterinary Medicine

This cross-sectional study was introduced to determine the risk factors of subclinical mastitis in dairy farms. Sixteen small holder farms and 285 milking cows in Khon Kaen province were selected for the study. The mastitis investigation, feeding information and clinical inspection were applied in the farm visits. Milk samples from each quarter with a CMT score of 3 and bulk milk were collected for bacterial identification and somatic cell counts. The prevalence of subclinical mastitis and associated factors were analyzed. Results found that the prevalence of subclinical mastitis was 36.14%. Major microorganisms isolated from infected quarters were Streptococcus spp. and …

Duplex Pcr For Simultaneous And Unambiguous Detection Of Streptococcus Iniae And Streptococcus Agalactiae Associated With Streptococcosis Of Cultured Tilapia In Thailand, Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat, Janenuj Wongtawatchai

The Thai Journal of Veterinary Medicine

Warm-water streptococcosis outbreaks in Thai cultured tilapia are caused by Streptococcus iniae and S.agalactiae. However, distinguishing between these two species of streptococcus are very difficult due to their similarmicrobiological appearance and clinical signs of infected fish. In this study, we proposed a new duplex-PCR basedmethod for simultaneous detection of these pathogens. The duplex-PCR amplified partial lctO and 16s rRNA gene ofS. iniae and S. agalactiae at 870 bp and 220 bp, respectively. This technique gave 100% specificity while sensitivity ofreaction was 100 fg of each bacterial genomic DNA. Detection limit of duplex-PCR applicable to clinical specimens isalso evaluated as 106 …

Acute And Subchronic Toxicity Study Of Ardisia Ellipica Thunb. Fruit Extract, Nalinphat Saktiyasunthorn, Songpol Chivapat, Pornchai Sincharoenpokai, Anudep Rungsipipat, Nongnuth Maneechai, Boonyanee Suphaphon, Aussavashai Shuayprom

The Thai Journal of Veterinary Medicine

Several plants species in the genus Ardisia contained various biologically active compounds. Ram Yai orPilangkasa (Ardisia elliptica Thunb.) has been used in traditional medicines. The objective of this study was to evaluatethe safety of ethanolic extract of A. elliptica fruit in animal models by oral administration. Acute toxicity test in miceby gavage the extract at each dose of 2.5 g/kg twice revealed no abnormal signs, mortality and gross lesion of vitalorgans. Subchronic toxicity study was investigated in one hundred Wistar rats separated into five groups, each oftwenty (ten males and ten females). Two control groups received distilled water and 0.5% …

Seroprevalence Of Neospora Caninum In Swamp Buffaloes And Beef Cattle In The North-East Of Thailand, Hoai Nam Nguyen, Aran Chanlun, Kwankate Kanistanon, Suneerat Aiumlamai

The Thai Journal of Veterinary Medicine

A seroepidemiological survey was carried out to investigate seroprevalence of Neospora caninum in swamp buffaloes from five provinces in north-eastern Thailand, and to compare infection status in swamp buffaloes and beef cattle at one area of Thailand. A single serum sample was collected from each of 532 swamp buffaloes and 78 beef cattle during January and July 2009. Antibodies against N. caninum were analyzed by a commercial ELISA test. Chisquare tests were used to compare the seroprevalence between species, gender and age groups in Khon Kaen. Result showed that infection prevalence in swamp buffaloes in northeastern Thailand was 4.5%. At …

Ecg Quiz, Chollada Buranakarl, Kittipong Tachampa

The Thai Journal of Veterinary Medicine

No abstract provided.

Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmidprofiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-sixplasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most commonpatterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2.3, 1.7, 1.3, 1, 0.7 Mda. The results indicate widedistribution of plasmids among the commensal E. coli isolates originated from clinically-healthy pigs.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Cloning And Prokaryotic Expression Of Cdnas From Hepatitis E Virus Structural Gene Of The Sw189 Strain, Bao-Cheng Hao, Xi Lan, Xiao-Yong Xing, Hai-Tao Xiang, Feng-Qin Wen, Yu-Yao Hu, Yong-Hao Hu, Jian-Ping Liang, Ji-Xing Liu

The Thai Journal of Veterinary Medicine

It is necessary to study the second open reading frame (open reading frame 2, ORF2) and antigenic peptidescharacteristic of genotype 4 of HEV, and create one kind of high sensitivity and specificity of anti-HEV IgM detectionkit. The objective of this study was to obtain recombinant antigen for development of anti-HEV ELISA method andvaccine against hepatitis E virus infection. A 728 base cDNA was collected from 5'-terminus of open reading frame 2(ORF2) among epidemic hepatitis E virus (HEV) isolated from Gansu, Western China. The fragment was digestedwith Not I and Nco I, and inserted into vector pET32a(+). The recombinant plasmid was …

The Therapeutic Effects Of Prednisolone In Cats With Immune-Mediated Hemolytic Anemia, Rosama Pusoonthornthum, Parkorn Limlenglert, Pitcha Pornmingmas, Anudep Rungsipipat, Pinit Pusoonthornthum

The Thai Journal of Veterinary Medicine

The present study was performed to find the therapeutic effects of 2 mg/kg prednisolone orally fed twice a day in cats with immune-mediated hemolytic anemia (IMHA). Six IMHA positive cats were diagnosed with Coomb’s test. Mean age of IMHA cats was 4.4 years old. They were considered as having secondary IMHA. The mortality rate of IMHA cats received prednisolone treatment was 33.3%. Complete blood count and blood chemistry values were all within the normal limit.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.