Veterinary Medicine Commons^™

Training Needs Of Licensed Veterinary Technicians Employed In Augusta County, Virginia, Jeannie Kay Skelton

OTS Master's Level Projects & Papers

The problem of this study was to determine the training needs of Licensed Veterinary Technicians in Augusta County, Virginia to improve their knowledge and skills in the veterinary field. The study was guided by research questions that were developed to determine what the training needs of Licensed Veterinary Technicians are, what their preferred method is for obtaining continuing education credits, what methods they use to obtain new knowledge and skills, and if on the job training is effective. A survey was designed to determine what training methods are being used, what is preferred, and what training is needed in the …

Evaluating The Physical Welfare Of Dogs In Commercial Breeding Facilities In The United States, Moriah J. Hurt

Open Access Theses

Despite high levels of public concern, the state of commercial breeding (CB) dog welfare is largely unknown. Therefore, this study aimed to characterize the physical welfare of dogs in CB facilities and their environment in Indiana. This study specifically aimed 1) to characterize dog foot health in CB facilities as a function of the length of time dogs were housed on a given flooring substrate, 2) to characterize visual dog body cleanliness and visual kennel cleanliness as a function of the flooring substrate, 3) to determine the efficacy of kennel cleaning procedures and 4) to characterize the dog dental and …

Influence Of Dietary Component Manipulation And Feed Management Strategies On Growth And Rumen Development Of Weaned Dairy Heifers, Tana Shea Dennis

Open Access Dissertations

Well-developed replacement heifers provide a central foundation for the continued success of the dairy industry. Emphasis on improving pre-weaned calf nutrition has predominated in the industry, but opportunities exist to improve post-weaning heifer nutrition and management. We aimed to evaluate common feed management strategies seen in the industry and their effects on growth, feed efficiency (G:F), and rumen development of calves from birth to 8 mo of age using pen- and individually-fed animal trials. Little information exists regarding post-weaning performance and rumen development of calves fed conventional or high planes of nutrition pre-weaning; therefore, we evaluated two milk replacer feeding …

Evaluating The Prevalence And Effectiveness Of Breed-Specific Legislation, Felicia E. Trembath

Open Access Dissertations

Dog bites pose a persistent public health problem, which some jurisdictions pass breed-specific legislation (BSL) to address. However, very little non-anecdotal evidence regarding the efficacy of BSL has been presented. Currently, BSL research is hampered by the absence of standard terminology, an established prevalence, or a scientific consensus on its effectiveness. The purpose of this study is to propose standardized terminology for BSL, establish the prevalence of each type of BSL in the USA, and conduct a systematic review of the effectiveness of BSL.

After review of terminology currently in use, as well as review of the regulatory actions of …

Development Of A Novel Ex Vivo Equine Corneal Model, Todd L. Marlo, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective

To develop an ex vivo equine corneal organ culture model. Specifically, to assess the equine cornea's extracellular matrix and cellularity after 7 days using two different culture techniques: either (i) immersion system or (ii) air/liquid interface system, to determine the best ex vivo equine corneal model.

Animals Studied

Fourteen healthy equine corneas of various breeds.

Procedures

Equine corneas with 2 mm of perilimbal sclera were freshly harvested from 7 horses undergoing humane euthanasia. One corneal–scleral ring (CSR) from each horse was randomly placed in the (i) immersion condition organ culture system (IC), with the contralateral CSR being placed in …

Growing South Dakota (Summer 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Breaking New Ground SDSU Leads Nation With Precision Agriculture Initiative
[Page] 6 In The Driver's Seat Utility Vehicles Provide Students Hands-On Precision Ag Experience
[Page] 7 Introduction To Industry Travel To National Events Opens Opportunities For Students
[Page] 9 On The Ground SDSU Extension Specialists Have Vital Role In Bringing Data To Producers
[Page] 10 Curricula Collaboration SDSU Leaders Among Team Preparing Future Precision Ag Coursework
[Page] 12 Future Focus Ag 2020 Plan Prompts Important Reinvestments In State's Ag Experiment Station
[Page] 13 The Power Of Connections Connected Approach Helps Solve "Real-Life" Problems To Benefit Animal And …

Enhanced Bovine Colostrum Supplementation Shortens The Duration Of Respiratory Disease In Thoroughbred Yearlings, Clara K. Fenger, Thomas Tobin, Patrick J. Casey, Edward A. Roualdes, John L. Langemeier, Ruel Cowles, Deborah M. Haines

Maxwell H. Gluck Equine Research Center Faculty Publications

Bovine colostrum (BC) is used in humans as a nutritional supplement for immune support and has been shown to reduce Respiratory disease (RD). Other nutritional supplements, minerals and vitamins including mannan oligosaccharides (MOS), zinc and vitamins A, C and E have also been used for immune support. The aim of this prospective blinded randomized clinical trial was to evaluate the effects of a BC, MOS, zinc and vitamin based enhanced bovine colostrum supplement (BCS) on incidence and duration of RD occurring in yearling horses. 109 yearlings on two Thoroughbred farms in Central Kentucky were randomly assigned to treatment or placebo …

Large Deletions At The Shox Locus In The Pseudoautosomal Region Are Associated With Skeletal Atavism In Shetland Ponies, Nima Rafati, Lisa S. Andersson, Sofia Mikko, Chungang Feng, Terje Raudsepp, Jessica Pettersson, Jan Janecka, Ove Wattle, Adam Ameur, Gunilla Thyreen, John E. Eberth, John Huddleston, Maika Malig, Ernest Bailey, Evan E. Eichler, Göran Dalin, Bhanu Chowdary, Leif Andersson, Gabriella Lindgren, Carl-Johan Rubin

Maxwell H. Gluck Equine Research Center Faculty Publications

Skeletal atavism in Shetland ponies is a heritable disorder characterized by abnormal growth of the ulna and fibula that extend the carpal and tarsal joints, respectively. This causes abnormal skeletal structure and impaired movements, and affected foals are usually killed. In order to identify the causal mutation we subjected six confirmed Swedish cases and a DNA pool consisting of 21 control individuals to whole genome resequencing. We screened for polymorphisms where the cases and the control pool were fixed for opposite alleles and observed this signature for only 25 SNPs, most of which were scattered on genome assembly unassigned scaffolds. …

Diversity Of Non-Flavobacterium Columnare Bacteria Associated With Columnaris-Like Diseased Fish, Ha Thanh Dong, Vuong Viet Nguyen, Wanniwat Mata, Pattanapon Kayansamruaj, Saengchan Senapin, Dachrit Nilubol, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Numerous yellow and pink pigmented bacterial isolates had been recovered from diseased fish exhibiting columnaris-like disease in tilapia (Oreochromis spp.) farms in Thailand. The predominant species had been identified as Flavobacterium columnare while taxonomic classification of the remaining isolates and their pathogenic potential remain undetermined. An additional yellow bacterial strain had also been obtained from a koi carp sample showing columnaris-like symptoms. To continue our previous work, this study described the identification of ten representatives of unknown culturable non-Flavobacterium columnare bacteria based on a combination of phenotypic characteristics and nucleotides homology of 16S rRNA gene and subsequently investigated their pathogenicity …

Identification Of Novel Snps And 10 Bp Deletion Of Ovine Dgat1 Gene And Their Association With Milk Production Traits, Ozge Ozmen, Selim Kul

The Thai Journal of Veterinary Medicine

Acyl coenzyme A: diacylglycerol acyltransferase 1 (dgat1) plays a key role in triacylglycerol synthesis by catalyzing the final committed step in the formation of triglycerides. Moreover, it could be used as a positional marker gene associated with milk production and composition traits. The purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine dgat1 gene and its possible association with milk traits in dairy sheep breeds. The genetic structures of SNPs were examined by PCR-RFLP and DNA sequencing in three sheep populations. Significant association of dgat1 gene of the intron 16 g.8384T>C loci …

Optimization Of Trehalose Concentration In Semen Freezing Extender In Thai Native Chicken Semen, Pachara Thananurak, Thevin Vongpralup, Chamaiporn Sittikasamkit, Kedsirin Sakwiwatkul

The Thai Journal of Veterinary Medicine

The aim of this study was to find optimal concentration of trehalose supplemented to semen extenders (0, 5, 10, and 15 mM). Ejaculates were collected from 24 Thai native cocks (Pradu Hang Dam; 1 year old), then diluted (1:3) and pooled before allocation to treatments. In the experiment, semen was either cooled to 5ºC after dilution or diluted with a pre-cooled diluents before cooling to 5ºC. Semen freezing was performed in liquid nitrogen vapor at -135ºC and -35ºC, with 6% DMF, and compared for sperm qualities. After thawing, it was measured for total sperm motility and progressive motile by CASA. …

Urethral Rupture And Leakage Following Prolonged Surgical Removal Of Urethral And Bladder Stones By Episiotomy And Urethrotomy In Female Asian Elephant (Elephas Maximus) : A Case Report, Nikorn Thongtip, Benchapol Lorsunyaluck, Manakorn Sukmak, Sirinart Chaichanathong, Pornchai Sunyathitiseree

The Thai Journal of Veterinary Medicine

A 46-year-old female Asian elephant (Elephas maximus) with an abnormal ballottement mass in the perineal area was presented. The elephant was unable to urinate via the normal tract. Previously, this elephant was treated for urethral and bladder obstruction caused by stones by episiotomy and urethrotomy. Eight months later, post-operative care was completed. At this point, the incision wound completely closed and the elephant could urinate through the normal tract. However, the elephant was then mated with an adult male elephant. Three weeks later, the female showed signs of the perineal ballottement mass and was unable to urinate. Episiotomy and urethrotomy …

Molecular Detection Of Theileria And Babesia In A Diversity Of Stomoxyini Flies (Diptera: Muscidae) From Khao Yai National Park, Thailand, Tanasak Changbunjong, Sivapong Sungpradit, Oraphan Kanthasaewee, Poonyapat Sedwisai, Siriporn Tangsudjai, Jiraporn Ruangsittichai

The Thai Journal of Veterinary Medicine

Blood protozoan species of the genera Theileria and Babesia are known to infect both domestic and wild animals. This study was carried out to detect Theileria and Babesia DNA in Stomoxyini flies collected in Khao Yai National Park, Thailand. A total of 110 Stomoxyini flies including 6 species, Stomoxys calcitrans, Stomoxys indicus, Stomoxys pullus, Stomoxys uruma, Haematobosca sanguinolenta and Haematostoma austeni, were examined by amplification against the 18S rRNA gene of the Theileria/Babesia species and then the PCR products were sequenced for species identification by comparison with published sequences from the GenBank database. Ten (9.1%) out of 110 samples were …

Pulp-Dentin Complex Response To Ru-Hbm1, A Novel Resin Modified Glass Ionomer Cement Prototype, In Deep Cavity Preparation Of Porcine Teeth, Wijit Banlunara, Siriporn Songsiripadapboon, Sirithan Jiemsirilers, Onusa Saravari, Dujreutai Pongkao Kashima, Nonglax Thunyakitpisal, Pasakorn Brikshavana, Phanitnan Srisuwan, Thitima Rupunt, Pasutha Thunyakitpisal

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the response of the pulp-dentin complex to Research Unit-Herbal Medicine, Biomaterial and Material 1 (RU-HBM1), a resin-modified glass ionomer (RMGI) cement prototype, in deep cavity of porcine teeth compared with commercial RMGIs. Six 14-week-old pigs were used in this study. Deep class V cavity was created on the buccal surface of canine, premolar, and molar teeth. The teeth were randomly lined with the design cement bases: RU-HBM1, Vitrebond (VB), or GC-Gold Label Light-Cured Universal Restorative (GC). After that, the cavity walls were treated with 30% phosphoric acid and washed. Then, a bonding …

Protective Efficacy Of Live Lasota Strain Newcastle Disease Virus Vaccine In Layer-Type Chickens, Jiroj Sasipreeyajan, Phornphisut Areeraksakul, Somkid Khanda

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the protective efficacy of live LaSota strain Newcastle disease virus (NDV) vaccine administered with different routes against a NDV challenge in layer-type chickens. Fifty male layer-type chickens were divided into 3 groups. There were 20 chickens/group for the vaccinated groups (Groups 1 and 2) and 10 chickens for the non-vaccinated control group (Group 3). Chickens in Groups 1 and 2 received the vaccine individually at 4 weeks old by eye drop and oral drop, respectively. All chickens received NDV challenge individually by oral drop at 7 weeks old or 3 weeks post-vaccination …

The Goat Semen Quality After Being Frozen Using Albumin And Cholesterol Substituted For Egg Yolk In Semen Extender, Terdchai Kaewkesa, Anucha Sathanawongs, Apichart Oranratnachai, Jureerat Sumretprasong

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the effect of albumin and cholesterol substituted for egg yolk in goat semen extender after frozen-thawed on semen quality and pregnancy rate. The semen was collected from three bucks by using artificial vagina. The semen was divided into 5 groups; in group 1, seminal plasma was removed and 20% egg yolk extender was added (standard), in group 2, 2% egg yolk extender was added into whole semen (control), in group 3, 4 mg/mL albumin extender was added into whole semen, in group 4, 5 mg/mL cholesterol extender was added into whole semen, …

Genetic Parameters Of Semen Quality Traits And Production Traits Of Pure-Bred Boars In Thailand, Krittaphak Buranawit, Nalinee Imboonta

The Thai Journal of Veterinary Medicine

The objective of this study was to estimate genetic parameters for semen quality and production traits of pure-bred Finnish-Norwegian boars raised in Thailand. Data used in this study were 9,760 records of semen collection and 108 records of performance tests (from 25 Duroc, 59 Landrace, and 24 Yorkshire boars). Semen quality traits considered per collection were semen volume (SV), semen concentration (SC), total sperm (TS), and total abnormality (TA). Data were recorded from 2001 to 2009, except for TA, which was not recorded until 2003. Production measures from each boar were average daily gain (ADG) and backfat thickness (BF). Results …

Allergen Sensitization Patterns Of Allergic Dogs: Ige-Microarray Analysis, Yun-Hsia Hsiao, Charles Chen, Ton Willemse

The Thai Journal of Veterinary Medicine

It was the aim of this study to determine sensitization rates to environmental allergens in 50 dogs with allergic dermatitis. Protein microarray of the dogs’ sera was used to identify allergen-specific IgE against 25 most common allergens. Increased IgE levels were most frequently observed for house dust mites (D. pteronyssinus 42.0%, D. farinae 38.0%) and storage mites (22-50%). In particular, the dogs with non-food-induced allergic dermatitis showed higher sensitization rates to mites compared to the dogs with food-induced allergic dermatitis, or a combination thereof. Reactions to insects were, with the exception of one dog sensitized to cockroach, entirely negative, and …

Identification Of Competitive Exclusion And Its Ability To Protect Against Campylobacter Jejuni In Broilers, Thotsapol Thomrongsuwannakij, Rungtip Chuanchuen, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study aimed to identify competitive exclusion (CE) isolated from native chickens and organic layers raised under non-antimicrobial usage farms. The protection of CE was tested against Campylobacter jejuni challenges in broilers. Lactobacillus spp., Bacillus spp. and Enterococcus faecium were identified from 50 adult chicken feces of those farms. According to antimicrobial-resistance concerns of European Food Safety Authority (EFSA), the use of CE that is susceptible to antimicrobials is preferred. The numbers of Lactobacillus spp., Bacillus spp. and Enterococcus faecium equal to 195, 93 and 58 strains, respectively, were tested for their minimum inhibitory concentrations (MICs) of 10 antimicrobials. As …

Ameliorative Effects Of Omega-3 Concentrate In Managing Coxofemoral Osteoarthritic Pain In Dogs, Amornrate Sastravaha, Nirut Suwanna, Chayakrit Sinthusingha, Jatuporn Noosud, Atthaporn Roongsitthichai

The Thai Journal of Veterinary Medicine

The aim of the present study was to investigate the effect of omega-3 concentrate on pain management in dogs with osteoarthritis (OA) at the coxofemoral joint. Totally, 31 dogs with confirmed coxofemoral OA were orthopedically evaluated for pain scores at walk and trot (1-6), joint manipulation (1-3), and examining range of motion (1-4). Besides, blood collection was performed on the first and the last visits. Omega-3 concentrate was orally given to all dogs at one capsule per 10 kgBW since the first visit on a daily basis for four consecutive weeks. On a weekly basis, pain scores were assessed for …

Bactericidal Effects Of Povidone-Iodine And Chlorhexidine Gluconate On Coagulase-Positive Staphylococci, Punpichaya Fungwithaya, Nuvee Prapasarakul

The Thai Journal of Veterinary Medicine

We aimed to evaluate the in vitro bactericidal efficacy of two routine antiseptics used in veterinary hospital, povidone-iodine and chlorhexidine gluconate in isopropanol (CGI),against canine coagulase-positive staphylococci (CoPS). Twenty CoPS were divided into 5 methicillin-resistant Staphylococcus pseudintermedius (MRSP) and 5 isolates each of S. pseudintermedius, S. aureus and S. schleiferi subsp. coagulans defined as methicillin-susceptible CoPS (MSCoPS). The bactericidal efficacy was determined by broth microdilution according to European Standard EN 1656:2000 at concentrations of 0.1, 1 and 10% PI and 0.5, 1 and 2% CGI for 15s, 30s, 45s, 1 min, 3 min and 5 min, respectively. There was no …

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.

Seroprevalence And Phylogenetic Analysis Of Toxoplasma Gondii From Domestic Cats, Captive Wild Felids, Free-Range Wild Felids And Rats In Certain Regions Of Thailand, Ruangrat Buddhirongawatr, Kridsada Chaichoun, Siriporn Tungsudjai, Ruenruethai Udonsom, Andrew Thompson, Oangart Mahittikorn, Paron Dekumyoy, Yaowalark Sukthana

The Thai Journal of Veterinary Medicine

Toxoplasmosis is an important zoonotic disease caused by Toxoplasma gondii, an obligated zoonotic apicomplexan parasite. The infection varies according to geographical areas. This work aimed to study the seroprevalence and genotype of T. gondii infection in domestic, captive and free-range wild felids, and in their small mammal prey, rats (Rattus spp). Two hundred and ninety three sera, received from the 4 individual animal groups in Thailand, were tested using the indirect latex agglutination test (ILAT) for specific antibody detection. The nested-PCR for glycerol-3-phosphate (B1) and bradyzoite surface antigen (SAG4) gene detection was used to detect seropositive animals and PCR product …

Electrocardiographic Changes In Dogs With Degenerative Mitral Valve Disease Treated With Pimobendan: A Retrospective Study Of 29 Cases, Sirilak Disatian Surachetpong, Nisarat Boonlue, Pasorn Pupa, Waratsarin Boonsathitanan, Sumanee Rakthaidee, Tanawan Mangklabruks, Siriwan Sakarin

The Thai Journal of Veterinary Medicine

The aim of this retrospective study was to evaluate changes in electrocardiograms (ECG) and cardiac arrhythmias in 29 dogs with degenerative mitral valve disease (DMVD) treated with pimobendan chronically. All dogs had normal ECG before pimobendan administration. The dogs were classified according to ECG after pimobendan administration into 2 groups: normal ECG (n=19) and abnormal ECG (n=10). Age, sex, breed, serum alkaline phosphatase, concurrent digoxin administration, total daily dosage of pimobendan and digoxin, duration of pimobendan administration, and ECG parameters including heart rate, P wave, PR interval, QRS complex, and QT interval were analyzed. Cardiac arrhythmias were increasingly found in …

Comparative Proteomic Analysis Of Cow Placentas With Retained Foetal Membranes, Shixin Fu, Yao Liu, Tiantian Nie, Chengyuan Zheng, Xiao Zou, Chunhai Luo, Jianying Liu

The Thai Journal of Veterinary Medicine

Retained foetal membrane (RFM) is a common reproductive pathology that harms the health of dairy cows. The aim of this study was to clarify the pathogenesis of RFM and screen for an early clinical diagnostic marker of this disease by using comparative proteomics and bioinformatics. Three RFM placental tissues from three cows and an equal number of normal placental tissues were collected for this study. There were 240 spots showing significant differential expressions of known proteins between the two types of foetal placental tissue samples. Between the two maternal placental tissue samples, there were 214 spots with differentially expressed levels …

Selection Of Reference Genes For Real-Time Polymerase Chain Reaction In Canine Oral Tumor And Cancer, Sirinun Pisamai, Anudep Rungsipipat, Chanin Kalpravidh, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

Tumors in oral cavity either benign or malignant are often found in dogs. Despite the increasing number of studies of gene expression associated with canine oral cavity diseases, reference gene selection for normalizing target genes has not been performed. The objective of the present study was to identify potential reference genes in canine oral tumors, including epulis, ameloblastoma, oral melanoma, oral squamous cell carcinoma, etc. Suitability of 6 candidate reference genes, beta-actin (ACTB), beta-2-microglobulin (B2M), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal subunit L13a (RPL13a), ribosomal protein S5 (RPS5) and ribosomal protein S19 (RPS19), was evaluated with 5 algorithms, geNorm, Normfinder, BestKeeper, the …

Quantitative Method For Detecting Vibrio Parahaemolyticus Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

This is the first report on the quantitative method for detecting Vibrio parahaemolyticus using the Bio-Theta DOXTM system. Seven V. parahaemolyticus strains and 23 non-V. parahaemolyticus strains were assessed. The rate of positive detection of Vibrio in samples of the 7 V. parahaemolyticus strains at high concentration (105 cfu/ml), medium concentration (103 cfu/ml), and low concentration (10 cfu/ml) was 100%. All 7 V. parahaemolyticus strains displayed good linear calibration curves for detection time relative to bacterial count (r > 0.993). The mean negative rate for the high-concentration (106 cfu/ml) non-V. parahaemolyticus samples was 65.2% (15/23) and that for the medium-concentration (103 …

Diagnosis Of Pharyngeal Sialocele In A Dog By Ct Scan : A Case Report, Sekkarin Ploypetch, Nan Choisunirachon, Chanin Kalpravidh

The Thai Journal of Veterinary Medicine

A 7-year-old, 10 kg male poodle dog was presented at the Small Animal Teaching Hospital, with the clinical signs of chronic coughing, bad deglutition and severe respiratory distress. On oral examination, a large mass arisen from the throat was found. Because the skull-cervical radiographs revealed only a large soft tissue pharyngeal mass which were difficult to indicate the mass types and the tissue invasiveness, the computed tomography (CT) was subsequently applied. The CT result showed that the mass was an encapsulated hypoattenuation mass (2.5 x 4.3 x 2.4 cm) at the right pharyngeal area without any adjacent soft tissue invasions. …