Bioresource and Agricultural Engineering Commons^™

Network Analysis Of Endogenous Gene Expression Profiles After Polyethyleneimine-Mediated Dna Delivery, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background—DNA delivery systems, which transport exogenous DNA to cells, have applications that include gene therapy, tissue engineering and medical devices. Although the cationic nonviral DNA carrier polyethyleneimine (PEI) has been widely studied, the molecular factors and pathways underlying PEI-mediated DNA transfer remain largely unknown, preventing the design of more efficient delivery systems.

Methods—HEK 293 T cells were treated with polyplexes formed with PEI and pEGFPLuc encoding for green fluorescent protein (GFP). Transfected cells expressing GFP were flowseparated from treated, untransfected cells. Gene expression profiles were obtained using Affymetrix HG-U133 2.0 microarrays and differentially expressed genes were identified using R/ Bioconductor. …

Fate And Transport Of Antimicrobials And Antimicrobial Resistance Genes In Soil And Runoff Following Land Application Of Swine Manure Slurry, Stacy R. Joy, Shannon L. Bartelt-Hunt, Daniel D. Snow, John Gilley, Brian L. Woodbury, David B. Parker, David B. Marx, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Due to the use of antimicrobials in livestock production, residual antimicrobials and antimicrobial resistance genes (ARGs) could enter the environment following the land application of animal wastes and could further contaminate surface and groundwater. The objective of this study was to determine the effect of various manure land application methods on the fate and transport of antimicrobials and ARGs in soil and runoff following land application of swine manure slurry. Swine manure slurries were obtained from facilities housing pigs that were fed chlortetracyline, tylosin or bacitracin and were land applied via broadcast, incorporation, and injection methods. Three rainfall simulation tests …

Review Of Us And Eu Initiatives Toward Development, Demonstration, And Commercialization Of Lignocellulosic Biofuels, Venkatesh Balan, David Chiaramonti, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

Advanced biofuels produced from lignocellulosic biomass offer an exciting opportunity to produce renewable liquid transportation fuels, biochemicals, and electricity from locally available agriculture and forest residues. The growing interest in biofuels from lignocellulosic feedstock in the United States (US) and the European Union (EU) can provide a path forward toward replacing petroleum-based fuels with sustainable biofuels which have the potential to lower greenhouse gas (GHG) emissions. The selection of biomass conversion technologies along with feedstock development plays a crucial role in the commercialization of next-generation biofuels. There has been synergy and, even with similar basic process routes, diversity in the …

"Apps"—An Innovative Way To Share Extension Knowledge, Joseph S. Dvorak, Tanya C. Franke-Dvorak, Randy R. Price

Biosystems and Agricultural Engineering Faculty Publications

Extension professionals across the country are continuously seeking innovative ways to reach clientele and to disseminate timely, educational information. A new avenue to reach clientele includes the use of smartphone "apps." The "Machinery Sizing" app, which was developed to ease the estimation of tractor horsepower to implement sizing for Extension clientele anytime, anywhere, is explained as a key example for Extension professionals to utilize apps in disseminating information to clientele. There are many benefits to using apps, including information availability wherever Internet service is available on the smartphone, ease of computations of equations, and automatic updates being sent to users.

Design, Fabrication And Manufacturing Analysis Of A Rollover Protection System, Nikolas Paris

BioResource and Agricultural Engineering

This senior project discusses the design, fabrication and manufacturing analysis of a rollover protection system specifically designed for early body style Ford Broncos (1966-1977). The rollover protection system, or roll cage, is an integral safety feature on vehicles which commonly encounter extreme terrain conditions. Many vehicles which attempt to traverse terrain such as this are not always properly equipped with a rollover protection system. This is the case with the early body style Ford Bronco. This system will be designed to market the niche of early bronco parts.

Bioflocculation Of Wastewater Treatment Pond Suspended Solids, Louis Lefebvre

Master's Theses

Bioflocculation of Wastewater Treatment Pond Suspended Solids

Louis Lefebvre

Wastewater treatment lagoons and high rate algae ponds (HRAPs) can provide cost effective wastewater treatment, but they commonly have high effluent concentrations of total suspended solids (TSS). In this thesis algae pond effluent was treated in a beaker testing apparatus (mixed and aerated) with various mixtures of activated sludge and primary effluent simulating differing activated sludge aeration basin compositions then was allowed to settle to assess settleability. Conventionally, microalgal suspended solids are removed by chemical coagulation followed by separation methods that often have a high cost relative to the low cost …

Long-Term Trends In Air Temperature Distribution And Extremes, Growing Degree-Days, And Spring And Fall Frosts For Climate Impact Assessments On Agricultural Practices In Nebraska, Kari E. Skaggs, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Air temperature influences agricultural practices and production outcomes, making detailed quantifications of temperature changes necessary for potential positive and negative effects on agricultural management practices to be exploited or mitigated. Temperature trends of long-term data for five agricultural locations, ranging from the subhumid eastern to the semiarid western parts of Nebraska, were studied to determine local temperature changes and their potential effects on agricultural practices. The study quantified trends in annual and monthly average maximum and minimum air temperature (T_max and T_min), daily temperature range (DTR), total growing degree-days, extreme temperatures, growing-season dates and lengths, and …

Modernization Of House #3: Improving Greenhouse Cut Flower Production And Students' Experience By Modernizing House #3, Kristina Anton

Biomedical Engineering

This paper documents the modernization of the black cloth, lighting, shade, and support systems in one of Cal Poly's on-campus, student operated cut flower production greenhouses. Problems with the old system are examined along with design requirements for a new system. Alternate designs are considered along with the installation process of the chosen design.

Developing A Restorable Wetland Index For Rainwater Basin Wetlands In South-Central Nebraska: A Multi-Criteria Spatial Analysis, Zhenghong Tang, Xu Li, Nan Zhao, Ruopu Li, F. Edwin Harvey

Community and Regional Planning Program: Faculty Scholarly and Creative Activity

It is always challenging for decision makers to prioritize wetland conservation programs at the landscape scale. This study employed a GIS-based multi-criteria spatial decision support tool that identified locations with the highest restoration potential for wetland conservation programs in the Rainwater Basin in south-central Nebraska. Five indicators were considered to assess wetland restoration potential: (1) Vegetation characteristics; (2) Soil characteristics; (3) Water volume released from hydrological modification of agricultural irrigation pits; (4) Topographical depression status; and (5) Habitat condition. The results suggested 192 (1.6% of the total) hydric soil footprints as the highest prioritized locations for future wetland restoration programs. …

Initial Changes In Species Cover Following Savanna Restoration Treatments In Western Iowa, David A. Mckenzie, Thomas B. Bragg, David M. Sutherland

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Study areas in the Iowa Loess Hills were used to evaluate short-term responses of understory species to three treatment methods designed to facilitate restoration of Quercus macrocarpa savanna. Treatments included burning alone, burning with thinning, and burning with clear-cutting. Plant abundance and diversity were compared before treatment and one year after treatment. Ninety-nine plant species were identified during the study, of which 40 were new following treatment, although most of these were forest associates. Increases in diversity of understory species were observed after treatment, particularly in plots with combined burning and thinning. The forb group was most consistent in response …

Characterizing Physical Properties Of Gas-Phase Biofilter Media, Guilherme Del Nero Maia, G. Tatiana Sales, George B. Day V, Richard S. Gates, Joseph L. Taraba

Biosystems and Agricultural Engineering Faculty Publications

Gas-phase biofiltration is an effective technology for reduction of odors and trace-gas contaminants. Significant contributions to the technical literature regarding the characterization of biofilter media have been generated in the past two decades. Nevertheless, the information produced has not been systematically organized. The objective of this study is to demonstrate and document methods for physical characterization of gas-phase compost biofilters (GPCB). The inclusion of moisture content, compaction, and particle size effects in the determination of media bulk density and porosity, field capacity, drying rate analysis, water sorption isotherms, and resistance to airflow is demonstrated. Results indicated that: (1) higher moisture …

Sbx7 Flow Rate Measurement Compliance For Agricultural Irrigation Districts, Charles M. Burt, Evan Geer

BioResource and Agricultural Engineering

Senate Bill x7-7 (SBx7-7) requires documented volumetric accounting to individual turnouts for water deliveries. Section 597.3 of the bill lists two very different requirements for devices. This document examines the requirements of SBx7 and the implications for agricultural irrigation districts. The definitions and impacts of flow rate and volumetric accuracy are defined. Example scenarios and solutions are offered, as well as descriptions of applicable devices.

Water And Energy Conservation Grow System: Aquaponics And Aeroponics With A Cycle Timer, Roger Hancock

Electrical Engineering

What some experts call the “blue gold”; water is a major issue in this world. There is only so much water and the reliability and cleanliness of water is what many developing countries are dealing with today. Countries around the world are facing problems with reliable water to grow enough food for their villages and homes. Is there is a way to minimize water consumption while optimizing growth in plants for food? One solution can be seen through both Aeroponics and Aquaponics. This project explores the potential of providing larger quantities of food to areas where water may be in …

Effects Of Sprinkler, Partial Sprinkler/Drip, And Drip Only Irrigation On Strawberry Transplants 2011-2012, Stuart Styles

BioResource and Agricultural Engineering

The Cal Poly Irrigation Training and Research Center (ITRC), in collaboration with academic, water district, and industry partners, proposes to evaluate new strategies for drip irrigation on strawberries to minimize runoff during transplant establishment.

The most common method used for irrigating strawberries is a mix of sprinkler irrigation and drip. Growers use sprinkler irrigation for bed preparation and salinity control, then eventually switch to drip irrigation after transplanting, but continue to use sprinkler irrigation as an insurance policy (for bonding between plant roots and soil bonding, washing off the leaves, controlling salinity, and frost). However, field observations have shown that …

Hydrologic Evaluation Of Established Rain Gardens In Lincoln, Nebraska, Andrew R. Anderson, Thomas G. Franti, David P. Shelton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Increased urbanization has resulted in water quality and flooding problems for many receiving waters in the United States. Rain gardens are one type of best management practice commonly used in low impact development (LID). Many studies have evaluated large engineered bioretention cells in research settings. There is little information on the effectiveness of homeowner-maintained rain gardens that rely on deep percolation as the method for water exfiltration. Repeatable controlled experiments are very rare in hydrologic studies due to the inherent variability of weather data. The objective of this study was to evaluate the hydrologic properties of twelve established rain gardens …

Dynamics Of Depression Storage During Sprinkler Irrigation And Precipitation Events, Michael B. Mckinney

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Surface depression storage (D_s) is the volume of precipitation excess which is stored by surface microrelief features of soils. The volume of water stored in surface depressions when precipitation rate exceeds infiltration rate reduces the amount of runoff generated. Because Ds is dependent on soil microrelief, land slope, and crop residue, tillage and management practices can have a considerable impact on the magnitude of this value.

When modeling irrigation systems and surface hydrology, depression storage is often treated as a static abstraction, meaning that maximum storage volume must be filled before runoff occurs. However, several researchers have documented …

Life Cycle Boundaries And Greenhouse Gas Emissions From Beef Cattle, Quentin M. Dudley

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Beef cattle are estimated to directly contribute 26% of U.S. agricultural greenhouse gas (GHG) emissions, and future climate change policy may target reducing these emissions. Life cycle assessment (LCA) of GHG emissions from U.S. feedlot beef cattle was conducted to compare methods of the U.S. Environmental Protection Agency (EPA) with a more complete evaluation of emissions. The inclusion of emissions from crop production for feed, associated land use change, and other minor factors nearly doubled GHG emissions associated with beef feedlots from the EPA Annual Inventory estimate of 1611 kgCO₂e hd^-1 yr^-1 to 3182 ± 167 …

Implementation Of Magnetic Meters For Irrigation Volumetric Measurement, Stuart Styles, Lynn Groundwater

BioResource and Agricultural Engineering

The irrigation industry is experiencing a growth in the use of magnetic meters for measuring the flow rate and volume in irrigation pipelines. Historically, propeller meters have been the device selected by users. New legislation in California (SB7x7) will require measurement devices at key locations for irrigation water delivery. Some users are very interested in the magnetic meter for making the measurement at the turnout or farm gate. The key feature of the new meter is the ability for the device to work in less than ideal flow conditions. Electromagnetic meters have been tested by the Irrigation Training and Research …

Observations Of Localised Effects Of The Busselton Compensating Basins On Surface Water, Groundwater And Soil Salinity, D L. Bennett, Richard J. George Dr

Resource management technical reports

Between 2001 and 2009 the Water Corporation constructed three flood-compensating basins on farmland in the Vasse and Sabina river catchments, upstream of in the city of Busselton. The basins are designed to reduce the risk of flooding in urban areas of Busselton by temporarily storing and then regulating runoff during flood events. This report fulfils the reporting requirements of a contract between the Water Corporation and the Department of Agriculture and Food. Data relating to the local groundwater and surface-water dynamics, salinity, nutrients and pH, plus changes to shallow and deep-soil salinity (from repeat ground-based electromagnetic induction [EM] surveying), was …

Irrigation System Evaluation And Pump Efficiency, Gregory Borges

BioResource and Agricultural Engineering

This project encompasses the Evaluation of a drip irrigation system for a Pistachio orchard in Tulare, Ca. The project parameters were decided on by Borges Farms. After the evaluation suggestions are to be made for improving the system’s efficiency. These suggestions must be approved by the grower and show whether they are cost efficient. The irrigation system cannot be modified to fit the pump chosen.

Research for the project encompasses drip irrigation systems, irrigation pump efficiency, irrigation management, well reclamation techniques and pistachio water requirements. A Distribution Uniformity evaluation was performed to understand how sand has affected the system. I …