Bioresource and Agricultural Engineering Commons^™

Induction Of Cellulase In High Solids Cultivation Of Trichoderma Reesei For Enhanced Enzymatic Hydrolysis Of Lignocellulose, Danielle Empson

Theses and Dissertations--Biosystems and Agricultural Engineering

This project aimed investigated cellulase in-situ production for large-scale on-farm production of lignocellulosic biofuel. Cellulase activity and glucose released by T. reesei with corn stover and wheat bran as co-substrates for solid state cultivation (SSC) were examined. Co-cultivation has previously increased T. reesei cellulase, but corn stover and wheat bran have not been co-cultivated (Dhillon, Oberoi et al. 2011). This work compared cellulase activity and glucose concentration of corn stover co-cultivated with 0-40% wheat bran in high solids. Samples with at least 20% wheat bran exhibited increased cellulase activity. However, the average glucose concentration without wheat bran was 3.29 g/L …

Development Of Regional And Hydraulic Geometry Curves For The Eastern Kentucky Coalfields, Ashlan Berry

Theses and Dissertations--Biosystems and Agricultural Engineering

Regional curves and hydraulic geometry curves relate bankfull channel dimensions to drainage area and bankfull discharge, respectively. These curves are used in the natural channel design process to help identify bankfull and to estimate bankfull dimensions of the design channel. Nineteen streams were surveyed to determine their bankfull parameters (cross-sectional area, width, mean depth, discharge, slope, and Manning’s n), along with 27 streams previously surveyed in other studies. The data were used to create regional and hydraulic geometry curves for three hydrologic landscape regions (HLR 9, HLR 11, and HLR 16, individually) in the Eastern Kentucky Coalfields (EKC) as well …

Experimental Investigation Of Fast Pyrolysis Of Arundo Donax In A Novel Paddle Reactor, Chintan Desai

Dissertations, Master's Theses and Master's Reports

The main objective of this research was to show the effectiveness of a novel paddle reactor for fast pyrolysis and study the effect of biomass particle size and reaction temperature of Arundo Donax a type of energy crop on the bio-oil yields. The novel paddle reactor showed effective mixing and heat transfer which were proved with the help of studying the residence time and the heating characteristics of the system respectively. The effect of different biomass particle sizes on the bio-oil yield was not significant, the bio-oil yield was approximately 50% for all the sizes. For Arundo Donax particle sizes …

Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya

Theses and Dissertations--Biosystems and Agricultural Engineering

The organism Clostridium thermocellum grows on cellulosic substrates and produces ethanol, acetate, lactate, formic acid, and CO₂. The organic acids produced alter the growth environment in which the bacteria grows and ultimately inhibit bacterial growth. One method which has been used successfully to maintain the system at acceptable growth conditions is to intermittently flush out the spent media and metabolic products and replace with new fermentation media. Our goal was to design and build an automated system that will automatically flush the spent media from the growing culture and resupply new media without manual intervention. An automated control …

Exploitation Of Food Industry Waste For High Value Products, Rajeev Ravindran, Amit Jaiswal

Articles

A growing global population calls for an increasing demand for food production and processing industry associated with it, and consequently generation of large amounts of food waste. This problem is further intensified due to slow progress in the development of effective waste management strategies, and less measure for the proper treatment and disposal of waste. Food waste is a reservoir of complex carbohydrates, proteins, lipids and nutraceuticals and can form the raw materials for commercially important metabolites. The current legislation on food waste treatment prioritises the prevention of waste generation and least emphasises disposal. Recent valorisation studies for food supply …

Mapping The Membrane Proteome Of Anaerobic Gut Fungi Identifies A Wealth Of Carbohydrate Binding Proteins And Transporters, Susanna Seppälä, Kevin V. Solomon, Sean P. Gilmore, John K. Henske, Michelle A. O'Malley

School of Agricultural & Biological Engineering Faculty Publications

Background

Engineered cell factories that convert biomass into value-added compounds are emerging as a timely alternative to petroleum-based industries. Although often overlooked, integral membrane proteins such as solute transporters are pivotal for engineering efficient microbial chassis. Anaerobic gut fungi, adapted to degrade raw plant biomass in the intestines of herbivores, are a potential source of valuable transporters for biotechnology, yet very little is known about the membrane constituents of these non-conventional organisms. Here, we mined the transcriptome of three recently isolated strains of anaerobic fungi to identify membrane proteins responsible for sensing and transporting biomass hydrolysates within a competitive and …

Increasing Student Attentiveness And Engagement In Growing Mechanized Systems Management Courses, Derek M. Heeren

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Background Growing enrollment in undergraduate MSYM courses has carried increased class sizes and then made it more difficult for students to remain attentive and engaged during lecture. In fall 2014, I maintained a dynamic lecturing style in MSYM 354, Soil Conservation and Watershed Management. Readiness tests, an active learning tool, continued to be helpful, but the mode of delivery was still largely a traditional lecture. Team based learning was utilized which divided the class into diverse learning teams for both the lab and the lecture. Students completed readiness tests both individually and in their teams. The objective of this activity …

In-Field Fuel Use And Load States Of Agricultural Field Machinery, Santosh Pitla, Joe D. Luck, Jared Werner, Nannan Lin, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The ability to define in-field tractor load states offers the potential to better specify and characterize fuel consumption rate for various field operations. For the same field operation, the tractor experiences diverse load demands and corresponding fuel use rates as it maneuvers through straight passes, turns, suspended operation for adjustments, repair and maintenance, and biomass or other material transfer operations. It is challenging to determine the actual fuel rate and load states of agricultural machinery using force prediction models, and hence, some form of in-field data acquisition capability is required. Controller Area Networks (CAN) available on the current model tractors …

Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this paper a fuzzy logic navigation controller for an inter-row agricultural robot is developed and evaluated in laboratory settings. The controller receives input from one-dimensional (1D) ranging sensors on the robotic platform, and operated on ten fuzzy rules for basic row-following behavior. The control system was implemented on basic hardware for proof of concept and operated on a commonly available microcontroller development platform and open source software libraries. The robot platform used for experimentation was a small tracked vehicle with differential steering control. Fuzzy inferencing and defuzzification, step response and cross track error were obtained from the test conducted …

Quantifying The Fate Of Agricultural Nitrogen In An Unconfined Aquifer: Stream-Based Observations At Three Measurement Scales, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder, Briant A. Kimball, Helena Mitasova, Francois Birgand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

We compared three stream-based sampling methods to study the fate of nitrate in groundwater in a coastal plain watershed: point measurements beneath the streambed, seepage blankets (novel seepage-meter design), and reach mass-balance. The methods gave similar mean groundwater seepage rates into the stream (0.3–0.6 m/d) during two 3–4 day field campaigns despite an order of magnitude difference in stream discharge between the campaigns. At low flow, estimates of flowweighted mean nitrate concentrations in groundwater discharge ([NO^-₃ ]_FWM) and nitrate flux from groundwater to the stream decreased with increasing degree of channel influence and measurement scale, i.e., …

Groundwater Transit Time Distribution And Mean From Streambed Sampling In An Agricultural Coastal Plain Watershed, North Carolina, Usa, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder

Department of Agricultural and Biological Systems Engineering: Faculty Publications

We measured groundwater apparent age (s) and seepage rate (v) in a sandy streambed using point-scale sampling and seepage blankets (a novel seepage meter). We found very similar MTT estimates from streambed point sampling in a 58 m reach (29 years) and a 2.5 km reach (31 years). The TTD for groundwater discharging to the stream was best fit by a gamma distribution model and was very similar for streambed point sampling in both reaches. Between adjacent point-scale and seepage blanket samples, water from the seepage blankets was generally younger, largely because blanket samples contained a fraction of ‘‘young’’ stream …

Impact Of Scale/Resolution On Evapotranspiration From Landsat And Modis Images, Vivek Sharma, Ayse Kilic, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Understanding the role of landscape heterogeneity and its influence on the scaling behavior of surface fluxes as observed by satellite sensors with different spatial resolutions is a critical need to investigate. In this study, the effects of pixel scales on ETc estimation and other parameters that are used to calculate ETc were investigated over different vegetation surfaces in south central Nebraska, USA. Surface Energy Balance System (SEBS) was used to estimate spatially distributed ETc by combining ground-based meteorological data for Landsat and MODIS imagery. The estimated surface energy fluxes were compared and validated to the measured Bowen Ratio Energy Balance …

262. Direct Measure Of Cervical Interbody Forces In Vivo: Load Reversal After Plating, Eric H. Ledet, Josh Peterson, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Darryl J. Dirisio

Department of Agricultural and Biological Systems Engineering: Faculty Publications

BACKGROUND CONTEXT: Biomechanics play an important role in spine fusion, but the in vivo biomechanics of the cervical spine are not well characterized and the in vivo biomechanics after spinal arthrodesis have never been studied. Load sharing facilitates fusion, but overloading of interbody implants can lead to subsidence and failure. In vitro studies have demonstrated that anterior plating significantly alters mechanical loading in the cervical spine. The instantaneous axis of rotation is shifted anteriorly and loading is reversed relative to an uninstrumented spine; the interbody space is compressed during extension and unloaded during flexion. However, this has never been tested …

Moisture Insensitive Prediction Of Soil Properties From Vnir Reflectance Spectra Based On External Parameter Orthogonalization, Nuwan K. Wijewardane, Yufeng Ge, Cristine L.S. Morgan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Moisture is the single most important factor that affects soil reflectance spectra, particularly for field applications. Interest in using soil VNIR spectral libraries, which are commonly based on dry ground soils, to predict soils in the intact field-moist condition (in situ VNIR) is growing. External parameter orthogonalization (EPO) has been proposed as a useful method that links dry ground VNIR models to field moist scans. The goal of this study is to test EPO on a wider set of soil properties and four different modeling techniques, namely, Partial Least Squares Regression (PLS), Artificial Neural Network (ANN), Random Forest (RF), and …

Neural Encoding Of Saltatory Pneumotactile Velocity In Human Glabrous Hand, Hyuntaek Oh, Rebecca Custead, Yingying Wang, Steven M. Barlow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Neurons in the somatosensory cortex are exquisitely sensitive to mechanical stimulation of the skin surface. The location, velocity, direction, and adaptation of tactile stimuli on the skin's surface are discriminable features of somatosensory processing, however the representation and processing of dynamic tactile arrays in the human somatosensory cortex are poorly understood. The principal aim of this study was to map the relation between dynamic saltatory pneumatic stimuli at discrete traverse velocities on the glabrous hand and the resultant pattern of evoked BOLD response in the human brain. Moreover, we hypothesized that the hand representation in contralateral Brodmann Area (BA) 3b …

A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Collecting plant phenotypic data with sufficient resolution (in both space and time) and accuracy represents a long standing challenge in plant science research, and has been a major limiting factor for the effective use of genomic data for crop improvement. This is particularly true in plant breeding where collecting large-scale field-based plant phenotypes can be very labor intensive and costly. In this paper we reported a multi-sensor system for high throughput phenotyping in plant breeding. The system comprised five sensor modules (ultrasonic distance sensors, thermal infrared radiometers, NDVI sensors, portable spectrometers, and RGB web cameras) to measure crop canopy traits …

Recalibration Methodology To Compensate For Changing Fluid Properties In An Individual Nozzle Direct Injection Systems, Joe D. Luck, S. A. Shearer, B. D. Luck, M. P. Sama

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were …

A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Wetlands are the largest global natural methane (CH₄) source, and emissions between 50 and 70° N latitude contribute 10–30% to this source. Predictive capability of land models for northern wetland CH₄ emissions is still low due to limited site measurements, strong spatial and temporal variability in emissions, and complex hydrological and biogeochemical dynamics. To explore this issue, we compare wetland CH₄ emission predictions from the Community Land Model 4.5 (CLM4.5-BGC) with siteto regional-scale observations. A comparison of the CH₄ fluxes with eddy flux data highlighted needed changes to the model’s estimate of aerenchyma area, which …

Cellulase Immobilization On Superparamagnetic Nanoparticles For Reuse In Cellulosic Biomass Conversion, Qing Song, Yu Mao, Mark Wilkins, Fernando Segato, Rolf Prade

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report co-immobilization of two types of cellulases, β-glucosidase A (BglA) and cellobiohydrolase D (CelD), on sub-20 nm superparamagnetic nanoparticles. The nanoparticles demonstrated 100% immobilization efficiency for both BglA and CelD. The total enzyme activities of immobilized BglA and CelD were up to 67.1% and 41.5% of that of the free cellulases, respectively. The immobilized BglA and CelD each retained …

Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The need for accurate, real-time, reliable, and multi-scale soil water content (SWC) monitoring is critical for a multitude of scientific disciplines trying to understand and predict the Earth’s terrestrial energy, water, and nutrient cycles. One promising technique to help meet this demand is fixed and roving cosmic-ray neutron probes (CRNPs). However, the relationship between observed low-energy neutrons and SWC is affected by local soil and vegetation calibration parameters. This effect may be accounted for by a calibration equation based on local soil type and the amount of vegetation. However, determining the calibration parameters for this equation is labor- and time-intensive, …