Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey,
2017
TÜBİTAK
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …
Impact Of Different Housing Systems And Age Of Layers On Egg Quality Characteristics,
2017
TÜBİTAK
Impact Of Different Housing Systems And Age Of Layers On Egg Quality Characteristics, Bi̇lgehan Yilmaz Di̇kmen, Aydin İpek, Ümran Şahan, Arda Sözcü, Süleyman Can Baycan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate effects of conventional-cage (CC), enriched-cage (EC), and free-range (FR) systems and hen age on internal and external egg quality parameters of layers (Lohmann Brown). A total of 720 eggs were analyzed for egg weight (EW), shell weight (SW), yolk weight (YW), albumen weight (AW), shell thickness (ST), shell breaking strength (SBS), shape (SI), albumen (AI), yolk index (YI) of eggs, shell ratio of eggs (SR), albumen ratio of eggs (AR), yolk ratio of eggs (YR), yolk color (YC), and Haugh unit (HU). The highest EW, YW, AW, SW, AI, YI, HU (all …
Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs,
2017
TÜBİTAK
Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …
Hematological Effects Of Etomidate And Tricaine In Common Carp,
2017
TÜBİTAK
Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska
Turkish Journal of Veterinary & Animal Sciences
Hematological effects of etomidate and tricaine at concentrations causing general anesthesia or sedation of carp juveniles were compared. Recovery of body balance took similar time in the case of both anesthetics, but fish treated with etomidate were depressed for several hours, while those subjected to tricaine immediately regained normal behavior. Immediately after exposure anesthetics caused minor hematological changes; the only statistically significant effects (decrease of hematocrit and plasma glucose) occurred after sedation with etomidate. One week after exposure a significant increase in mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration occurred in all groups of fish compared to the previous …
Effects Of Nigella Sativa And Hypericum Perforatum On Wound Healing,
2017
TÜBİTAK
Effects Of Nigella Sativa And Hypericum Perforatum On Wound Healing, Mehmet Cengi̇z Han, Ali̇ Sai̇d Durmuş, Aydin Sağliyan, Ci̇han Günay, Mustafa Özkaraca, Fati̇h Mehmet Kandemi̇r, Seli̇m Çomakli, Di̇cle Firat Öztopalan
Turkish Journal of Veterinary & Animal Sciences
In this study, it was aimed to determine the influence of applying Nigella sativa (NS) and Hypericum perforatum (HP) on wound healing. A total of 42 female albino 2-month-old Wistar rats were randomly separated into 3 main groups. Each of these main groups was separated into two subgroups for 7- and 14-day postoperative follow-ups. A total of 84 excisional skin wounds, 5 mm in diameter, were created on the backs of the rats. Placebo cream (control group), 50% NS oil cream, and 50% HP oil cream were applied topically for the first, second, and third group, respectively. The wounds were …
Influence Of Dietary Vitamin D3 Supplementation On The Color Changes In Longissimus Lumborum, Vastus Lateralis, Semitendinosus, And Semimembranosus Beef Muscles, Urszula Karolina Rafalska
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effect of dietary vitamin D3 supplementation on changes in color and heme pigment (myoglobin and metmyoglobin) in 4 beef muscles. The muscles came from 48 crossbred bulls that were subjected to supplementation with vitamin D3 at 4 different doses: 0.0 (control group), 3.5, 7.0, and 10.0 x 106 IU. The 3.5 x 106 IU dose was administered daily for 6 days, before the morning feeding. Four days before slaughter, the supplementation was withdrawn. The dose of 7.0 and 10.0 x 106 IU was administered for 3 days and withdrawn 7 days …
Accelerated Achilles Tendon Healing With Interleukin-1 Receptor Antagonist Protein In Rabbits,
2017
TÜBİTAK
Accelerated Achilles Tendon Healing With Interleukin-1 Receptor Antagonist Protein In Rabbits, Marko Pecin, Mario Kreszinger, Snjezana Vukovic, Marija Lipar, Ozren Smolec, Berislav Radisic, Josip Kos
Turkish Journal of Veterinary & Animal Sciences
The current study describes the use of interleukin-1 receptor antagonist protein (IL-1Ra) as a possible strategy for optimizing tendon healing and repair by presenting histologically visible changes in rabbit Achilles tendon tissue after longitudinal tenotomy. The study was carried out on 26 Californian rabbits divided into two equal groups. One was the experimental IRAP (interleukin-1 receptor antagonist) group and the second one was the PBS (purified buffered saline) group. The PBS group was the control group. After longitudinal tenotomy in both groups of rabbits, in the IRAP group IL-1Ra was applied locally, whereas PBS was applied in the control group. …
Molecular Cloning And Sequence Characterization Of Two Genes, Dqa1 And Dqa2,From The Chinese Yakow (Bos Grunniens X Bos Taurus), Dongmei Xi, Sameeullah Memon, Guozhi Li, Xiangying Liu, Chao Su, Jing Leng, Liping Wang, Sehroon Khan, Weidong Deng
Turkish Journal of Veterinary & Animal Sciences
The major histocompatibility complex (MHC) plays a crucial role in the immune response to infectious diseases. The aim of the present study was to investigate the structural and functional characteristics and possible duplication of the MHC-DQA genes in the Chinese yakow, which is well adapted to the Qinghai?Tibetan Plateau. Two full-length cDNA sequences and partial 5'- and 3'-untranslated regions (UTRs) were retrieved and denoted as Bogr×BoLA-DQA*0101 (DQA1) and BogrxBoLA-DQA*2001 (DQA2), respectively. The percentage of nucleotide and amino acid sequences homology between BogrxBoLA-DQA1 and BogrxBoLA-DQA2 showed that these two sequences have more identity to alleles of the corresponding DQA1 and DQA2 …
Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant,
2017
TÜBİTAK
Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant, Harun Hizlisoy, Serhat Al, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan, Kadi̇r Semi̇h Gümüşsoy
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to investigate the presence of Escherichia coli O157 and to determine the antibiotic susceptibility of isolates in different poultry-originated materials. In total 300 samples of chicken carcass swabs (n = 50), chicken feces (n = 50), gizzard (n = 50), liver (n = 50), water in the final scald tank (n = 50), and equipment such as tables, conveyers, and knives (n = 50) were used as materials. Overall, 45 E. coli isolates were obtained from the 300 poultry samples. However, 13 (4.3%) of the samples were identified as E. coli O157 including 3 …
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China,
2017
TÜBİTAK
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning
Turkish Journal of Veterinary & Animal Sciences
Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …
Japanese Quail Performance, Intestinal Microflora, And Molecularresponses To Screened Wheat And Multienzyme Diet,
2017
TÜBİTAK
Japanese Quail Performance, Intestinal Microflora, And Molecularresponses To Screened Wheat And Multienzyme Diet, Ali Asghar Saki, Fatemeh Sahebi Ala, Pouya Zamani, Dariush Alipour, Masoumeh Abbasinezhad
Turkish Journal of Veterinary & Animal Sciences
The present paper studied the effect of screened wheat (SW) levels and a multienzyme diet on Japanese quail performance, intestinal performance, and microbial population. A total of 480 unsexed 10-day-old quail chicks were assigned to a multienzyme diet (0 or 0.5 g/kg) and SW (0%, 5%, 10%, or 15%) in a 2 x 4 factorial arrangement of treatments with 4 replications and 15 quails in each in a completely randomized design. No differences were observed in performance between diets at 10 to 36 days of age. Increases in dietary SW levels decreased villus height, villus width, villus surface, and height:crypt …
Normal Bone Healing In Three Cloned Dogs With Long Bone Fractures,
2017
TÜBİTAK
Normal Bone Healing In Three Cloned Dogs With Long Bone Fractures, Yawon Hwang, Sang-Hwan Hyun, Woo-Suk Hwang, Gonhyung Kim
Turkish Journal of Veterinary & Animal Sciences
A number of mammals have been cloned, including cats and dogs. The purpose of this report was to study the bone fracture and bone fracture healing of three cloned dogs. The three cloned dogs, aged 3 months to 6 months, received separate surgeries. Clinical and radiological examination of each of the three cloned dogs revealed the fracture of a femur, a tibial tuberosity, and a humerus, respectively. Each dog had undergone surgical reduction and stabilization of their individual bone fracture with an intramedullary pin, a screw, and/or a wire. No abnormalities were observed during the follow-up period. The dogs showed …
Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs,
2017
TÜBİTAK
Radiographic Examination Of Hip Joint Geometry In Healthy Sivas Kangal Dogs, Murat Sarierler, Zeynep Bozkan Tatli, İbrahi̇m Akin, Rahi̇me Yaygingül, Onur Özgün Deri̇ncegöz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the normal ranges of proximal femoral and acetabular parameters in 26 adult healthy Sivas Kangal dogs (SKDs). Significant differences were observed between the sexes in hip axis length (HAL) of 8.03 ± 0.82 cm and 7.47 ± 0.40 cm, femoral neck axis length-a (FNALa) of 6.40 ± 0.55 cm and 6.09 ± 0.27 cm, FNALb of 5.09 ± 0.47 cm and 4.78 ± 0.31 cm, acetabular width (AW) of 1.62 ± 0.35 cm and 1.37 ± 0.24 cm, femoral shaft diameter (FSD) of 3.11 ± 0.31 cm and 2.88 ± 0.21 cm, …
Investigation Of Effects Of Fucoidan Polysaccharides Extracted From Twospecies Of Padina On The Wound-Healing Process In The Rat, Moazameh Kordjazi, Bahareh Shabanpour, Ebrahim Zabihi, Mohammad Ali Faramarzi, Hassan Ahmadi Gavlighi, Seyed Mohammad Amin Feghhi, Seyed Abbas Hosseini
Turkish Journal of Veterinary & Animal Sciences
Seaweeds contains different types of polysaccharides with high abilities. The aim of this study was to determine the potential of fucoidan polysaccharides for wound-healing properties. Fucoidans of two species of Padina (P. tetrastromatica and P. boergesenii), collected from the Persian Gulf northern coastal area, were extracted and analyzed using high-performance anion-exchange chromatography. Purified fucoidans were used to prepare a 2% topical ointment. Under aseptic conditions, two identical paravertebral full-thickness skin wounds (either by burn or excision) were made in 40 anesthetized male rats allocated into 4 groups. Either fucoidan ointments or the vehicle (ointment base) were applied to rats' wounds …
Effects Of Ketamine On Monoamine Neurotransmittersand Gaba In Embryonic Neurocytes From Fetal Rat,
2017
TÜBİTAK
Effects Of Ketamine On Monoamine Neurotransmittersand Gaba In Embryonic Neurocytes From Fetal Rat, Xinran Li, Mo Liu, Yue Li, Cen Guo, Lina Li, Yanan Li, Yuxin Wang, Yiming Zhang, Li Gao
Turkish Journal of Veterinary & Animal Sciences
The effect of ketamine, a drug commonly used as an anesthetic, on monoaminergic neurotransmitters and GABA in neurocytes from fetal rats has not yet been elucidated. We thus decided to investigate these effects by first evaluating ketamine levels in the cerebrospinal fluid by high-performance liquid chromatography. We thus determined the concentrations of GABA, DA, NE, 5-HT, and 5-HIAA following the addition of four different concentrations of ketamine at day 7 of nerve cell culture. GABA, 5-HT, and 5-HIAA levels were increased after an initial decrease: they showed the lowest levels at 10-15 min and the highest peaks at 45-90 min, …
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells,
2017
Faculty of Veterinary Science
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease,
2017
Faculty of Veterinary Science
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease,
2017
Faculty of Veterinary Science
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats,
2017
Faculty of Veterinary Science
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, …
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin,
2017
Faculty of Veterinary Science
The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Leaky gut characterized as decreased transepithelial electrical resistance and increased paracellular permeability to macromolecules is associated with several fatal diseases including sepsis. Pro-inflammatory cytokine IL-1β is a critical mediator of underlying mechanism through activation of myosin light chain kinase pathway. Blocking the inducible phosphorylated myosin light chain (p-MLC) indicates the successful treatment. Xanthones predominantly found in mangosteen (Garcinia mangostana) has been indicated as a potent anti-inflammatory action. Hydroxyxanthones (HDX), the major natural xanthones, have been recently synthesized in various forms. This study aimed to examine and screen the action of various forms of synthetic HDXs, in preventing the increased paracellular …
