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Utilizing Chemical Mutagenesis To Determine U21 Mechanism Of Action In Toxoplasma Gondii, Andrew Pham 2020 University of Nebraska at Omaha

Utilizing Chemical Mutagenesis To Determine U21 Mechanism Of Action In Toxoplasma Gondii, Andrew Pham

Student Research and Creative Activity Fair

The abstract has been removed to protect intellectual property of the project.


Pyrosequencing Analysis Of Irs1 Methylation Levels In Schizophrenia With Tardive Dyskinesia, Yanli Li, Kesheng Wang, Ping Zhang, Junchao Huang, Ying Liu, Zhiren Wang, Yongke Lu, Shuping Tan, Fude Yang, Yunlong Tan 2020 Marshall University

Pyrosequencing Analysis Of Irs1 Methylation Levels In Schizophrenia With Tardive Dyskinesia, Yanli Li, Kesheng Wang, Ping Zhang, Junchao Huang, Ying Liu, Zhiren Wang, Yongke Lu, Shuping Tan, Fude Yang, Yunlong Tan

Pharmacology, Physiology and Toxicology

Tardive dyskinesia (TD) is a serious side effect of certain antipsychotic medications that are used to treat schizophrenia (SCZ) and other mental illnesses. The methylation status of the insulin receptor substrate 1 (IRS1) gene is reportedly associated with SCZ; however, no study, to the best of the authors' knowledge, has focused on the quantitative DNA methylation levels of the IRS1 gene using pyrosequencing in SCZ with or without TD. The present study aimed to quantify DNA methylation levels of 4 CpG sites in the IRS1 gene using a Chinese sample including SCZ patients with TD and without TD (NTD) and ...


Generation Of In-Frame Gene Deletion Mutants In Pseudomonas Aeruginosa And Testing For Virulence Attenuation In A Simple Mouse Model Of Infection, Meagan E. Valentine, Brandon D. Kirby, Hongwei D. Yu 2020 Marshall University

Generation Of In-Frame Gene Deletion Mutants In Pseudomonas Aeruginosa And Testing For Virulence Attenuation In A Simple Mouse Model Of Infection, Meagan E. Valentine, Brandon D. Kirby, Hongwei D. Yu

Biomedical Sciences

Microorganisms are genetically versatile and diverse and have become a major source of many commercial products and biopharmaceuticals. Though some of these products are naturally produced by the organisms, other products require genetic engineering of the organism to increase the yields of production. Avirulent strains of Escherichia coli have traditionally been the preferred bacterial species for producing biopharmaceuticals; however, some products are difficult for E. coli to produce. Thus, avirulent strains of other bacterial species could provide useful alternatives for production of some commercial products. Pseudomonas eruginosa is a common and well-studied Gram-negative bacterium that could provide a suitable alternative ...


Detection Of A Peptide Hormone - Somatostatin - Label-Free Split-Aptameric Probes, Charles A. Dowis 2020 University of Central Florida

Detection Of A Peptide Hormone - Somatostatin - Label-Free Split-Aptameric Probes, Charles A. Dowis

Honors Undergraduate Theses

Peptide hormones are important biomolecules that transduce downstream effects such as cell proliferation, regulation, and gene expression. Their levels have been upregulated in various disorders such as cancer, yet detection methods are lacking. We designed two split aptamer-based assays for the detection of a peptide hormone – Somatostatin (SST) – with different signal readouts: fluorescent readout based on light-up aptamers and the colorimetric readout of ABTS peroxidation from a G-quadruplex. We used an already selected split-aptamer –SSTA5–for SST for our designs and we had expected the developed detection systems to exhibit detection and quantification capabilities that would hopefully allow their use ...


Challenges And Opportunities With Causal Discovery Algorithms: Application To Alzheimer’S Pathophysiology, X. Shen, S. Ma, P. Vemuri, G. Simon, M. W. Weiner, P. Aisen, R. Petersen, C. R. Jack, P. Davies, V. Shibley, +90 additional authors 2020 Zucker School of Medicine at Hofstra/Northwell

Challenges And Opportunities With Causal Discovery Algorithms: Application To Alzheimer’S Pathophysiology, X. Shen, S. Ma, P. Vemuri, G. Simon, M. W. Weiner, P. Aisen, R. Petersen, C. R. Jack, P. Davies, V. Shibley, +90 Additional Authors

Journal Articles

© 2020, The Author(s). Causal Structure Discovery (CSD) is the problem of identifying causal relationships from large quantities of data through computational methods. With the limited ability of traditional association-based computational methods to discover causal relationships, CSD methodologies are gaining popularity. The goal of the study was to systematically examine whether (i) CSD methods can discover the known causal relationships from observational clinical data and (ii) to offer guidance to accurately discover known causal relationships. We used Alzheimer’s disease (AD), a complex progressive disease, as a model because the well-established evidence provides a “gold-standard” causal graph for evaluation. We ...


Machine Learning Prediction Of Glioblastoma Patient One-Year Survival, Andrew Du '20, Warren McGee, Jane Y. Wu 2020 Illinois Mathematics and Science Academy

Machine Learning Prediction Of Glioblastoma Patient One-Year Survival, Andrew Du '20, Warren Mcgee, Jane Y. Wu

Student Publications & Research

Glioblastoma (GBM) is a grade IV astrocytoma formed primarily from cancerous astrocytes and sustained by intense angiogenesis. GBM often causes non-specific symptoms, creating difficulty for diagnosis. This study aimed to utilize machine learning techniques to provide an accurate one-year survival prognosis for GBM patients using clinical and genomic data from the Chinese Glioma Genome Atlas. Logistic regression (LR), support vector machines (SVM), random forest (RF), and ensemble models were used to identify and select predictors for GBM survival and to classify patients into those with an overall survival (OS) of less than one year and one year or greater. With ...


Immunological Significance Of Hmgb1 Post-Translational Modification And Redox Biology, M. S. Kwak, H. S. Kim, B. Lee, Y. H. Kim, M. Son, J. S. Shin 2020 Zucker School of Medicine at Hofstra/Northwell

Immunological Significance Of Hmgb1 Post-Translational Modification And Redox Biology, M. S. Kwak, H. S. Kim, B. Lee, Y. H. Kim, M. Son, J. S. Shin

Journal Articles

© Copyright © 2020 Kwak, Kim, Lee, Kim, Son and Shin. Most extracellular proteins are secreted via the classical endoplasmic reticulum (ER)/Golgi-dependent secretion pathway; however, some proteins, including a few danger-associated molecular patterns (DAMPs), are secreted via non-classical ER/Golgi-independent secretion pathways. The evolutionarily conserved high mobility group box1 (HMGB1) is a ubiquitous nuclear protein that can be released by almost all cell types. HMGB1 lacks signal peptide and utilizes diverse non-canonical secretion mechanisms for its extracellular export. Although the post-translational modifications of HMGB1 were demonstrated, the oxidation of HMGB1 and secretion mechanisms are not highlighted yet. We currently investigated that ...


Prolonged Targeted Cardiovascular Epidural Stimulation Improves Immunological Molecular Profile: A Case Report In Chronic Severe Spinal Cord Injury, O. Bloom, J. M. Wecht, B. E. Ditterline, S. Wang, A. Ovechkin, C. A. Angeli, A. A. Arcese, S. J. Harkema 2020 Zucker School of Medicine at Hofstra/Northwell

Prolonged Targeted Cardiovascular Epidural Stimulation Improves Immunological Molecular Profile: A Case Report In Chronic Severe Spinal Cord Injury, O. Bloom, J. M. Wecht, B. E. Ditterline, S. Wang, A. Ovechkin, C. A. Angeli, A. A. Arcese, S. J. Harkema

Journal Articles

No abstract provided.


Ultrasound Powered Piezoelectric Neurostimulation Devices: A Commentary., T. Sun, J. Wright, T. Datta-Chaudhuri 2020 Zucker School of Medicine at Hofstra/Northwell

Ultrasound Powered Piezoelectric Neurostimulation Devices: A Commentary., T. Sun, J. Wright, T. Datta-Chaudhuri

Journal Articles

Conventional neurostimulation systems for preclinical research can be bulky and invasive due to the need for batteries or wired interfaces. Emerging as a new neural interface technique, ultrasound-powered piezoelectric neural stimulators work by converting ultrasound energy to electrical charge for neural stimulation. In addition to the benefits of wireless powering and miniaturization leading to less traumatic surgery, piezoelectric neural stimulators can also exhibit prolonged operational lifetimes for a long-term stable neural interface, and show promise for clinical translation. As one of first steps to demonstrate the value of ultrasound-powered piezoelectric neural interface, Li et al. developed a piezoelectric stimulator to ...


Lupus Autoantibodies Act As Positive Allosteric Modulators At Glun2a-Containing Nmda Receptors And Impair Spatial Memory, K. Chan, J. Nestor, T. S. Huerta, N. Certain, G. Moody, C. Kowal, P. T. Huerta, B. T. Volpe, B. Diamond, L. P. Wollmuth 2020 Zucker School of Medicine at Hofstra/Northwell

Lupus Autoantibodies Act As Positive Allosteric Modulators At Glun2a-Containing Nmda Receptors And Impair Spatial Memory, K. Chan, J. Nestor, T. S. Huerta, N. Certain, G. Moody, C. Kowal, P. T. Huerta, B. T. Volpe, B. Diamond, L. P. Wollmuth

Journal Articles

No abstract provided.


Mechanistic And Functional Shades Of Mucins And Associated Glycans In Colon Cancer, Ramesh Pothuraju, Shiv Ram Krishn, Shailendra K. Gautam, Priya Pai, Koelina Ganguly, Sanjib Chaudhary, Satyanarayana Rachagani, Sukhwinder Kaur, Surinder K. Batra 2020 University of Nebraska Medical Center

Mechanistic And Functional Shades Of Mucins And Associated Glycans In Colon Cancer, Ramesh Pothuraju, Shiv Ram Krishn, Shailendra K. Gautam, Priya Pai, Koelina Ganguly, Sanjib Chaudhary, Satyanarayana Rachagani, Sukhwinder Kaur, Surinder K. Batra

Journal Articles: Biochemistry & Molecular Biology

Mucus serves as the chief protective barrier against pathogenic and mechanical insults in respiratory, gastrointestinal, and urogenital tracts. Altered mucin expression, the major component of mucus, in conjunction with differential glycosylation has been strongly associated with both benign and malignant pathologies of colon. Mucins and their associated glycans arbitrate their impact sterically as well as mechanically by altering molecular and microbial spectrum during pathogenesis. Mucin expression in normal and pathological conditions is regulated by nonspecific (dietary factors and gut microbiota) and specific (epigenetic and transcriptional) modulators. Further, recent studies highlight the impact of altering mucin glycome (cancer-associated carbohydrate antigens including ...


A Cross-Disease Meta-Gwas Identifies Four New Susceptibility Loci Shared Between Systemic Sclerosis And Crohn’S Disease, D. González-Serna, E. Ochoa, E. López-Isac, A. Julià, F. Degenhardt, N. Ortego-Centeno, T. R. Radstake, P. K. Gregersen, A. T. Lee, A. Gabrielli, +78 additional authors 2020 Zucker School of Medicine at Hofstra/Northwell

A Cross-Disease Meta-Gwas Identifies Four New Susceptibility Loci Shared Between Systemic Sclerosis And Crohn’S Disease, D. González-Serna, E. Ochoa, E. López-Isac, A. Julià, F. Degenhardt, N. Ortego-Centeno, T. R. Radstake, P. K. Gregersen, A. T. Lee, A. Gabrielli, +78 Additional Authors

Journal Articles

© 2020, The Author(s). Genome-wide association studies (GWASs) have identified a number of genetic risk loci associated with systemic sclerosis (SSc) and Crohn’s disease (CD), some of which confer susceptibility to both diseases. In order to identify new risk loci shared between these two immune-mediated disorders, we performed a cross-disease meta-analysis including GWAS data from 5,734 SSc patients, 4,588 CD patients and 14,568 controls of European origin. We identified 4 new loci shared between SSc and CD, IL12RB2, IRF1/SLC22A5, STAT3 and an intergenic locus at 6p21.31. Pleiotropic variants within these loci showed opposite allelic ...


Neutrophil Extracellular Traps In Covid-19., Y Zuo, S Yalavarthi, H Shi, K Gockman, M Zuo, JA Madison, C Blair, A Weber, BJ Barnes, M Egeblad, RJ Woods, Y Kanthi, JS Knight 2020 Northwell Health

Neutrophil Extracellular Traps In Covid-19., Y Zuo, S Yalavarthi, H Shi, K Gockman, M Zuo, Ja Madison, C Blair, A Weber, Bj Barnes, M Egeblad, Rj Woods, Y Kanthi, Js Knight

Journal Articles

No abstract provided.


Eda2r Mediates Podocyte Injury In High Glucose Milieu, X. Lan, V. Kumar, A. Jha, R. Aslam, H. Wang, K. Chen, Y. Yu, W. He, A. Malhotra, P. C. Singhal, +2 additional authors 2020 Zucker School of Medicine at Hofstra/Northwell

Eda2r Mediates Podocyte Injury In High Glucose Milieu, X. Lan, V. Kumar, A. Jha, R. Aslam, H. Wang, K. Chen, Y. Yu, W. He, A. Malhotra, P. C. Singhal, +2 Additional Authors

Journal Articles

© 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM) EDA2R is a member of the large family of tumor necrosis factor receptor (TNFR). Previous studies suggested that EDA2R expression might be increased in the kidneys of diabetic mice. However, its mRNA and protein expression in kidneys were not analyzed; moreover, its role in the development of diabetic kidney disease was not explored. Here we analyzed the mRNA and protein expressions of EDA2R in diabetic kidneys and examined its role in the podocyte injury in high glucose milieu. By analysis with real-time PCR, Western blotting, we found ...


Pharmacological Prevention Of Surgery-Accelerated Metastasis In An Animal Model Of Osteosarcoma, M. P. Kallis, C. Maloney, B. Blank, S. Z. Soffer, M. Symons, B. M. Steinberg 2020 Northwell Health

Pharmacological Prevention Of Surgery-Accelerated Metastasis In An Animal Model Of Osteosarcoma, M. P. Kallis, C. Maloney, B. Blank, S. Z. Soffer, M. Symons, B. M. Steinberg

Journal Articles

No abstract provided.


Identification Of Unusual Phospholipids From Bovine Heart Mitochondria By Hplc-Ms/Ms., J. Kim, C. L. Hoppel 2020 Zucker School of Medicine at Hofstra/Northwell

Identification Of Unusual Phospholipids From Bovine Heart Mitochondria By Hplc-Ms/Ms., J. Kim, C. L. Hoppel

Journal Articles

Phospholipids, including ether phospholipids, are composed of numerous isomeric and isobaric species that have the same backbone and acyl chains. This structural resemblance results in similar fragmentation patterns by collision-induced dissociation of phospholipids regardless of class, yielding complicated tandem mass spectrometry (MS/MS) spectra when isobaric species are analyzed together. Furthermore, the presence of isobaric species can lead to misassignment of species when made solely based on their molecular weights. In this study, we used normal-phase HPLC for ESI-MS/MS analysis of phospholipids from bovine heart mitochondria. Class separation by HPLC eliminates chances for misidentification of isobaric species from different ...


Predictive Value Of 18F-Florbetapir And 18F-Fdg Pet For Conversion From Mild Cognitive Impairment To Alzheimer Dementia, G. Blazhenets, Y. Ma, A. Sörensen, F. Schiller, G. Rücker, D. Eidelberg, L. Frings, P. T. Meyer 2020 Northwell Health

Predictive Value Of 18F-Florbetapir And 18F-Fdg Pet For Conversion From Mild Cognitive Impairment To Alzheimer Dementia, G. Blazhenets, Y. Ma, A. Sörensen, F. Schiller, G. Rücker, D. Eidelberg, L. Frings, P. T. Meyer

Journal Articles

© 2020 by the Society of Nuclear Medicine and Molecular Imaging. The present study examined the predictive values of amyloid PET, 18F-FDG PET, and nonimaging predictors (alone and in combination) for development of Alzheimer dementia (AD) in a large population of patients with mild cognitive impairment (MCI). Methods: The study included 319 patients with MCI from the Alzheimer Disease Neuroimaging Initiative database. In a derivation dataset (n = 159), the following Cox proportional-hazards models were constructed, each adjusted for age and sex: amyloid PET using 18F-florbetapir (pattern expression score of an amyloid-β AD conversion-related pattern, constructed by principle-components analysis); 18F-FDG PET (pattern ...


Apol1 Polymorphism Modulates Sphingolipid Profile Of Human Podocytes, M. Valsecchi, V. Cazzetta, F. Oriolo, X. Lan, R. Piazza, M. A. Saleem, P. C. Singhal, D. Mavilio, J. Mikulak, M. Aureli 2020 Zucker School of Medicine at Hofstra/Northwell

Apol1 Polymorphism Modulates Sphingolipid Profile Of Human Podocytes, M. Valsecchi, V. Cazzetta, F. Oriolo, X. Lan, R. Piazza, M. A. Saleem, P. C. Singhal, D. Mavilio, J. Mikulak, M. Aureli

Journal Articles

No abstract provided.


Circulating Tnf-Like Protein 1a (Tl1a) Is Elevated Early In Rheumatoid Arthritis And Depends On Tnf, Y. Song, I. A. Choi, F. Meylan, M. K. Demoruelle, T. Farley, A. C. Richard, E. Hawley, J. Botson, P. Gregersen, R. M. Siegel, +19 additional authors 2020 Zucker School of Medicine at Hofstra/Northwell

Circulating Tnf-Like Protein 1a (Tl1a) Is Elevated Early In Rheumatoid Arthritis And Depends On Tnf, Y. Song, I. A. Choi, F. Meylan, M. K. Demoruelle, T. Farley, A. C. Richard, E. Hawley, J. Botson, P. Gregersen, R. M. Siegel, +19 Additional Authors

Journal Articles

No abstract provided.


Inhibition Of Irf5 Cellular Activity With Cell-Penetrating Peptides That Target Homodimerization, J. Banga, D. Srinivasan, C. Sun, C. D. Thompson, F. Milletti, K. Huang, S. Song, B. Matta, D. Li, B. J. Barnes, +17 additional authors 2020 Northwell Health

Inhibition Of Irf5 Cellular Activity With Cell-Penetrating Peptides That Target Homodimerization, J. Banga, D. Srinivasan, C. Sun, C. D. Thompson, F. Milletti, K. Huang, S. Song, B. Matta, D. Li, B. J. Barnes, +17 Additional Authors

Journal Articles

No abstract provided.


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