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Articles 7111 - 7140 of 9760

Full-Text Articles in Veterinary Medicine

Osmosensitive Taurine Release: Does Taurine Share The Same Efflux Pathway With Chloride And Other Amino Acid Osmolytes?, Rodrigo Franco Jan 2003

Osmosensitive Taurine Release: Does Taurine Share The Same Efflux Pathway With Chloride And Other Amino Acid Osmolytes?, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Swelling subsequent to hyposmotic conditions activates a process of volume regulation present in most cell types. This volume adjustment is accomplished by osmolyte translocation toward the extracellular space to reach a new osmotic equilibrium. Molecules involved in this homeostatic mechanism have been broadly classified into two categories: organic and inorganic osmolytes. Inorganic osmolytes comprise mainly the intracellular ions K+ and Cl–. Cell swelling–induced activation of separate K+ and Cl– channels has been described in most preparations. Organic osmolytes are grouped in three categories: amino acids, polyalcohols, and methylamines. These osmolytes, particularly taurine, are present in …

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Role Of Activator Protein-1 In The Down-Regulation Of The Human Cyp2j2 Gene In Hypoxia, Nicole Y. Marden, Eva Fiala-Beer, Shi-Hua Xiang, Michael Murray Jan 2003

Role Of Activator Protein-1 In The Down-Regulation Of The Human Cyp2j2 Gene In Hypoxia, Nicole Y. Marden, Eva Fiala-Beer, Shi-Hua Xiang, Michael Murray

School of Veterinary and Biomedical Sciences: Faculty Publications

The cytochrome P450 (CYP) 2J2 arachidonic acid epoxygenase gene was down-regulated at a pre-translational level in human hepatoma-derived HepG2 cells incubated in a hypoxic environment; under these conditions, the expression of c-Jun and c-Fos mRNA and protein was increased. The 5′-upstream region of the CYP2J2 gene was isolated by amplification of a 2341 bp fragment and putative regulatory elements that resembled activator protein-1 (AP-1)-like sequences were identified. From transient transfection analysis, c-Jun was found to strongly activate a CYP2J2–luciferase reporter construct, but co-transfection with plasmids encoding c-Fos or c-Fos-related antigens, Fra-1 and -2, abrogated reporter activity. Using a series …

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Mucosal Priming Of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses In Rhesus Macaques By The Salmonella Type Iii Secretion Antigen Delivery System, David T. Evans, Li-Mei Chen, Jacqueline Gillis, Kuei-Chin Lin, Brian Harty, Gail P. Mazzara, Ruben O. Donis, Keith G. Mansfield, Jeffrey D. Lifson, Ronald C. Desrosiers, Jorge E. Galán, R. Paul Johnson Jan 2003

Mucosal Priming Of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses In Rhesus Macaques By The Salmonella Type Iii Secretion Antigen Delivery System, David T. Evans, Li-Mei Chen, Jacqueline Gillis, Kuei-Chin Lin, Brian Harty, Gail P. Mazzara, Ruben O. Donis, Keith G. Mansfield, Jeffrey D. Lifson, Ronald C. Desrosiers, Jorge E. Galán, R. Paul Johnson

School of Veterinary and Biomedical Sciences: Faculty Publications

Nearly all human immunodeficiency virus (HIV) infections are acquired mucosally, and the gut-associated lymphoid tissues are important sites for early virus replication. Thus, vaccine strategies designed to prime virus-specific cytotoxic T lymphocyte (CTL) responses that home to mucosal compartments may be particularly effective at preventing or containing HIV infection. The Salmonella type III secretion system has been shown to be an effective approach for stimulating mucosal CTL responses in mice. We therefore tested ∆phoP-phoQ attenuated strains of Salmonella enterica serovar Typhimurium and S. enterica serovar Typhi expressing fragments of the simian immunodeficiency virus (SIV) Gag protein fused to the …

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Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray Jan 2003

Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention provides a method to detect the presence of a microorganism or agent in an animal. The method encompasses placement of devices at various locations where the animal resides so as to induce the animal to initiate contact with the device. As a result of this contact, the animal deposits various microorganisms and agents on the device. The device is then tested for the presence of the particular microorganism or agent of interest.

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Roles Of Mycobacterium Smegmatis D-Alanine:D-Alanine Ligase And D-Alanine Racemase In The Mechanisms Of Action Of And Resistance To The Peptidoglycan Inhibitor D-Cycloserine, Zhengyu Feng, Raul G. Barletta Jan 2003

Roles Of Mycobacterium Smegmatis D-Alanine:D-Alanine Ligase And D-Alanine Racemase In The Mechanisms Of Action Of And Resistance To The Peptidoglycan Inhibitor D-Cycloserine, Zhengyu Feng, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

D-Cycloserine (DCS) targets the peptidoglycan biosynthetic enzymes D-alanine racemase (Alr) and Dalanine: D-alanine ligase (Ddl). Previously, we demonstrated that the overproduction of Alr in Mycobacterium smegmatis determines a DCS resistance phenotype. In this study, we investigated the roles of both Alr and Ddl in the mechanisms of action of and resistance to DCS in M. smegmatis. We found that the overexpression of either the M. smegmatis or the Mycobacterium tuberculosis ddl gene in M. smegmatis confers resistance to DCS, but at lower levels than the overexpression of the alr gene. Furthermore, a strain overexpressing both the alr and ddl …

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Herpes Simplex Virus Type 1 And Bovine Herpesvirus 1 Latency, Clinton J. Jones Jan 2003

Herpes Simplex Virus Type 1 And Bovine Herpesvirus 1 Latency, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

A high percentage of the world’s population are infected with herpes simplex virus type 1 (HSV-1), and infection can cause a variety of disorders (35, 187). Recurrent ocular HSV-1 is the leading cause of infectious corneal blindness in industrialized nations (190). In a murine model, ocular infection induces autoimmune disorders, leading to corneal antigen destruction and stromal keratitis (275). HSV-1 infections also cause gastrointestinal disorders, esophageal disorders, and approximately 25% of the genital herpes infections (67, 158). HSV-1 infections can cause sporadic encephalitis, but this is relatively rare compared to other diseases resulting from infection. Further evidence for its involvement …

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Characterization Of Protein Secretion In Mycobacterium Leprae Using Phoa Fusions In Escherichia Coli And Mycobacterium Smegmatis, Marina Noemi Torrero Jan 2003

Characterization Of Protein Secretion In Mycobacterium Leprae Using Phoa Fusions In Escherichia Coli And Mycobacterium Smegmatis, Marina Noemi Torrero

LSU Doctoral Dissertations

Complete sequencing and annotation of the M. leprae genome has provided new information related to proteins constituting its hypothetical proteome. Since M. leprae can not be grown in vitro, novel approaches are needed to determine which proteins are expressed during infection and whether these proteins are related to pathogenesis. Secreted proteins represent a distinct group of protein with respect to their structure and function, contribution to virulence and are of particular importance for vaccine development because they are often immunogenic and have the potential to be recognized early in infection. The objectives of this study were: 1) to identify putatively …

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The Diagnostic Importance Of Erythrocyte Glutathione Peroxidase (Gsh-Px) Activity And Some Serum Parameters In Lambs With White Muscle Disease, M. Erman Or, H. Tamer Dodurka, Abdullah Kayar Jan 2003

The Diagnostic Importance Of Erythrocyte Glutathione Peroxidase (Gsh-Px) Activity And Some Serum Parameters In Lambs With White Muscle Disease, M. Erman Or, H. Tamer Dodurka, Abdullah Kayar

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the diagnostic importance of erythrocyte glutathione peroxidase activity (GSH-Px) in lambs with white muscle disease (WMD) and the changes in some serum parameters (glucose, creatinine, total protein, albumin, cholesterol, triglyceride, Ca, inorganic P, Na, K, Cl, AST, ALT, ALP, LDH and CPK) in healthy and white muscle diseased lambs. The research was carried out on 32 lambs of which 22 showed clinical symptoms and 10 were healthy from 280 lambs raised around Esenyurt-İstanbul. The erythrocyte glutathione peroxidase activity (GSH-Px) was determined using the RANSEL glutathione peroxidase test by taking approximately 7 cc …

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The Effects Of Some Silage Additives In Sorghum Silage On The Silage Quality And Ruminal Degradabilities Of Nutrients Ii- Ruminal Degradabilities Of Nutrients, N. Tuğba Bi̇ngöl, Erol Baytok Jan 2003

The Effects Of Some Silage Additives In Sorghum Silage On The Silage Quality And Ruminal Degradabilities Of Nutrients Ii- Ruminal Degradabilities Of Nutrients, N. Tuğba Bi̇ngöl, Erol Baytok

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine the effects of different silage additives on the ruminal degradability of sorghum silage. Different sorghum silages were prepared from sorghum harvested at the milk or dough stage (287.40 and 304.80 g/kg DM) with or without SIL-ALL (enzyme-inoculant complex; EI), molasses (M), formic acid (FA), M + FA, EI + FA, EI + M and EI + FA + M additives in laboratory silos (glass jar 1 kg in volume) and incubated for two months. After incubation, the silos were opened and dry matter and ADF analyses were done. The dry matter and …

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Detection Of Critical Control Points In White Cheese Production In Small Dairy Plants, Süreyya Saltan Evrensel, Seran Temelli̇, Şahsene Anar Jan 2003

Detection Of Critical Control Points In White Cheese Production In Small Dairy Plants, Süreyya Saltan Evrensel, Seran Temelli̇, Şahsene Anar

Turkish Journal of Veterinary & Animal Sciences

In this study, which was conducted to detect the critical control points and to investigate the direct and cross-contamination sources in white pickled cheese production, 21 control points were examined for aerobe mesophilic counts, staphylococcus-micrococcus, coagulase-positive staphylococcus, enterobacteriaceae, coliform bacteria, E. coli, enterococcus, yeast and mould counts. It was determined that the high microbiological load of raw milk affected the microbiological quality of pasteurized milk negatively, and that the production pipe-line after pasteurization, and equipment such as vats, plastic wraps, pressing cloths, starter culture and the hands of the workers, were direct contamination sources. In addition, the hands of workers …

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Theileriosis And Tick Infestations In Sheep And Goats Around Kayseri, Abdullah İnci̇, Serpi̇l Nalbantoğlu, Yücel Çam, Ayhan Atasever, K. Zafer Karaer, Ayşe Çakmak, Fahri̇ Sayin, Bayram Ali̇ Yukari, Anil İça, Ahmet Deni̇z Jan 2003

Theileriosis And Tick Infestations In Sheep And Goats Around Kayseri, Abdullah İnci̇, Serpi̇l Nalbantoğlu, Yücel Çam, Ayhan Atasever, K. Zafer Karaer, Ayşe Çakmak, Fahri̇ Sayin, Bayram Ali̇ Yukari, Anil İça, Ahmet Deni̇z

Turkish Journal of Veterinary & Animal Sciences

This study was conducted on sheep and goats to investigate theileriosis and tick infestations around Kayseri between August 1997 and October 1999. During this period a total of 300 small ruminants consisting of 250 sheep and 50 goats were microscopically examined for Theileria species. Blood smears were prepared from the punctured ear of each animal for microscopic examination. At the same time, ticks were collected from the animals. The smears were stained with 5% Giemsa's stain solution and then examined under microscope with oil immersion objective. Theileria sp. were detected in 46 (18.40% ) and 4 (8.00% ) of the …

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Serological And Microbiological Investigation On Turkey Rhinotracheitis Virus (Trtv) Infection Of Chickens And Turkeys, Yavuz Çokal, Ayşi̇n Şen Jan 2003

Serological And Microbiological Investigation On Turkey Rhinotracheitis Virus (Trtv) Infection Of Chickens And Turkeys, Yavuz Çokal, Ayşi̇n Şen

Turkish Journal of Veterinary & Animal Sciences

A total of 426 serum samples were examined from 9 broiler and 7 broiler breeder flocks showing clinical signs of swollen head syndrome (SHS). Turkey rhinotracheitis virus antibodies in 8 broiler flocks were between 31.8 and 75.9% and in broiler breeder flocks between 5.4 and 48.6%, with a commercial ELISA. Typical clinical symptoms were observed for SHS in broilers and broiler breeders. During the study, turkey flocks showing clinical signs were not detected. The antibodies to TRTV were between 5.5 and 80.0% in 7 out of 10 broiler flocks, and between 20.0 and 57.1% in 3 out of 4 fattening …

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Determination Of The Allele Frequency Of Some Genomic Loci In A Holstein Cattle Population And Its Importance In Individual Identification, Harun Ceri̇t Jan 2003

Determination Of The Allele Frequency Of Some Genomic Loci In A Holstein Cattle Population And Its Importance In Individual Identification, Harun Ceri̇t

Turkish Journal of Veterinary & Animal Sciences

Nowadays, the use of DNA sequences with genetic techniques and DNA polymorphism as genetic markers is increasing very rapidly. In this study, we examined the frequencies of seven different polymorphic loci in the cattle population of İstanbul University Veterinary Medicine Research and Application Farm. Thus, our aim was to examine and calculate the power of exclusion, match probability and power of discrimination of the seven loci, and examine the usefulness of the involved loci in identification tests. For DNA extraction, Chelex and Phenol-Chloroform-Isoamil alcohol methods were applied to the blood taken from animals. After measuring the amount and purity degree …

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The Effect Of Urea And Molasses Addition Into Corn Harvested At The Milk Stage On Silage Quality And Digestible Nutrient Yield, Hüseyi̇n Nursoy, Suphi̇ Deni̇z, Murat Demi̇rel, Ni̇hat Denek Jan 2003

The Effect Of Urea And Molasses Addition Into Corn Harvested At The Milk Stage On Silage Quality And Digestible Nutrient Yield, Hüseyi̇n Nursoy, Suphi̇ Deni̇z, Murat Demi̇rel, Ni̇hat Denek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of urea or urea plus molasses addition into corn harvested at the milk stage on the silage quality and in vitro digestibility of silage. To achieve this objective, four different corn varieties (Rx-947, 33-94, Frassino and Arifiye) harvested at the milk stage were utilized. Silage samples were added 0.5% urea or 0.5% urea plus 4% molasses and ensiled in mini-silos (1 L in volume). Silage samples were analyzed for chemical composition, pH and organic acids (lactic acid, acetic acid, propionic acid and butyric acids). In vitro dry matter (DM) digestibility …

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Investigation Of The Effects Of Age, Sex And Season On Electrocardiographs (Ecg Values) And Heart Radiographs Of Healthy Van Cats, Di̇de Kiliçalp, Ali̇ Çinar Jan 2003

Investigation Of The Effects Of Age, Sex And Season On Electrocardiographs (Ecg Values) And Heart Radiographs Of Healthy Van Cats, Di̇de Kiliçalp, Ali̇ Çinar

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the effects of age, sex and season change on electrocardiograms of Van cats. Thirty-eight (male and female) healthy Van cats, 3-15 months old were used. Electrocardiograms (ECGs) and heart radiographs were obtained from cats seperately in summer, autumn, winter and spring. ECGs were recorded as bipolar, augmented unipolar and precordial leads (CV_6LL, CV_6LU, V_10). Durations and amplitudes of waves on the trace were measured in lead II and the electrical axis also measured in leads I and III. Radiographic examination was performed in the right lateral recumbency position (covering the whole thoracic cavity) …

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A Comparative Investigation Of The Morphologic Relationship Between True Chordae Tendineae And False Chordae Tendineae Of The Left Ventricle In Different Species, Davut Özbağ, Pi̇raye Kervancioğlu, Berna Saruhan, Serhan Taşdemi̇r, Fahri̇ Yilmaz Jan 2003

A Comparative Investigation Of The Morphologic Relationship Between True Chordae Tendineae And False Chordae Tendineae Of The Left Ventricle In Different Species, Davut Özbağ, Pi̇raye Kervancioğlu, Berna Saruhan, Serhan Taşdemi̇r, Fahri̇ Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Our purpose in this study was to investigate and compare the morphological relationship between the true chordae tendineae (CT) and the false chordae tendineae (FCT). One hundred and forty-seven hearts (8 humans, 23 dogs, 91 sheep, 25 goats) were investigated in our study. In terms of CT number, while the difference between humans and sheep and goats, and dogs and sheep and goats was significant statistically (p < 0.05), the difference between humans and dogs was less significant than the others. The difference between sheep and goats was not significant statistically (p > 0.05). The number of FCT were not significant (p > 0.05) in the other species while the difference between humans and dogs was significant statistically. The numerical relationship between CT and FCT was positive …

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The Effects Of Gnrh + Pgf_{2\Alpha} And Hcg + Pgf_{2\Alpha} Applications In Postpartum Dairy Cows On Oestrus Syncronization And Fertility, Çağatay Tek, Ahmet Sabuncu, Alper Baran, Mi̇that Evecen Jan 2003

The Effects Of Gnrh + Pgf_{2\Alpha} And Hcg + Pgf_{2\Alpha} Applications In Postpartum Dairy Cows On Oestrus Syncronization And Fertility, Çağatay Tek, Ahmet Sabuncu, Alper Baran, Mi̇that Evecen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of GnRH + PGF_{2\alpha} and hCG + PGF_{2\alpha} applications on oestrus and fertility in dairy cows postpartum between days 35 and 45. In this study, 117 healthy, 3-7-year-old Holstein dairy cows from the same farm under similar husbandry conditions were used and they were in postpartum days between 35 and 45. Cows were divided equally into three groups randomly. Group I (n = 39): on day 0, 2000 IU hCG IM (Pregnyl^R, Organon) was given, on day 7, 500 \mug Cloprostenol Na IM (Estrumate^R, DİF), and on day 9, 12.6 …

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The Effects Of The Pressing And The Scalding Treatments On Some Quality Properties In Maraş Cheese Production, K. Kaan Teki̇nşen, Mustafa Ni̇zamlioğlu Jan 2003

The Effects Of The Pressing And The Scalding Treatments On Some Quality Properties In Maraş Cheese Production, K. Kaan Teki̇nşen, Mustafa Ni̇zamlioğlu

Turkish Journal of Veterinary & Animal Sciences

The study was designed to elicit some basic information on the effect of different pressing and scalding treatments applied to the curd with special reference to the standardization of Maraş cheese. For each experiment six different samples (IA, IB, IC, IIA, IIB, IIC) were used. The samples were manufactured experimentally by applying three different scalding treatments, 70 ± 1 ^oC/5 min. (A) , 75 ± 1 ^oC/5 min. (B) and 80 ± 1 ^oC/5 min (C), to each of two curds pressed for 1 h by 1:5 kg (I) and 1.5:5 kg (II) weights of milk used. All experimental samples …

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Studies On Some Haematological And Biochemical Parameters In Clinically Healthy Ostriches, Zahi̇d Ağaoğlu, Nazmi̇ Yüksek, Nuri̇ Altuğ, Abdullah Kaya, Burhanetti̇n Baydaş Jan 2003

Studies On Some Haematological And Biochemical Parameters In Clinically Healthy Ostriches, Zahi̇d Ağaoğlu, Nazmi̇ Yüksek, Nuri̇ Altuğ, Abdullah Kaya, Burhanetti̇n Baydaş

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the normal haematological and biochemical parameters of clinically healthy ostriches 1-3 years old of either sex. Red blood cells, white blood cells, haematocrit and haemoglobin values (as haematological parameters) and glucose, total protein, albumin, globulin, urea, kreatinine, serum enzymes (ALP, AST, ALT, GGT, CK, amilase) (as biochemical parameters) and electrolytes (Ca, P, Mg, Zn, Cu, Na, K, Cl) levels were measured. It was concluded that the values obtained in this study could be used as reference values for ostriches.

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The Effects Of High Copper And Zinc On Some Hematological Parameters In Rats, Sebahat Aydemi̇r, Mukaddes Özcan Jan 2003

The Effects Of High Copper And Zinc On Some Hematological Parameters In Rats, Sebahat Aydemi̇r, Mukaddes Özcan

Turkish Journal of Veterinary & Animal Sciences

The effect of high levels of Zn and Cu supplemented into the diet on Zn and Cu levels in the liver, pancreas and serum, the number of red and white blood cells, hematocrit value, hemoglobin levels, superoxide dismutase (SOD) activity in red blood cells, live weight, and liver and pancreas weights were investigated. A total of 77 Wistar albino rats were used as the research material. Twelve of the rats were separated as the control group and the remaining 65 were divided into five experimental groups (D1, D2, D3, D4 and D5) with 13 rats in each. Then, 70 mg/kg/day …

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Breeding Characteristics And Milk Yield Traits Of Holstein Cattle In Reyhanlı Agricultural Facility, Gali̇p Bakir, Mehmet Çeti̇n Jan 2003

Breeding Characteristics And Milk Yield Traits Of Holstein Cattle In Reyhanlı Agricultural Facility, Gali̇p Bakir, Mehmet Çeti̇n

Turkish Journal of Veterinary & Animal Sciences

In this study, the reproduction and milk yield production characteristics of 110 Holstein cows raised in the Reyhanlı Agricultural Facility are investigated. The study includes milk records obtained between 1990 and 1999 and reproduction records from 1993 to 1998. The aim of the study was to determine the descriptive values of milk yield and reproductive traits of Holstein Friesian cattle. The means for the breeding characteristics were as follows: age at first insemination 587.78 ± 102.67 days, age at first calving 892.12 ± 116.98 days, calving interval 394.01 ± 72.24 days, service period 103.39 ± 13.82 days, number of inseminations …

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The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek Jan 2003

The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek

Turkish Journal of Veterinary & Animal Sciences

The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with …

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Identification Of Salmonella Isolated From Freshwater Mussels (Unio Elongatulus Eucirrus Bourguignat, 1860) By Polymerase Chain Reaction, Engi̇n Şeker, Mustafa Sarieyyüpoğlu, Burhan Çeti̇nkaya Jan 2003

Identification Of Salmonella Isolated From Freshwater Mussels (Unio Elongatulus Eucirrus Bourguignat, 1860) By Polymerase Chain Reaction, Engi̇n Şeker, Mustafa Sarieyyüpoğlu, Burhan Çeti̇nkaya

Turkish Journal of Veterinary & Animal Sciences

In this study, 627 live freshwater mussels collected from the Koçkale and Pertek regions of Keban Dam Lake were examined for the presence of Salmonella spp. Homogenates prepared from the soft tissue of mussels, after pre-enrichment in Rappaport Vasilliadis, were inoculated onto Brilliant Green Phenol Red Lactose agar and Salmonella-Shigella selective media. Biochemical tests and DNA extraction were carried out on isolates suspected of containing Salmonella. From 19 (4.8%) out of 397 mussels collected in the Koçkale region, Salmonella spp. were isolated and identified, but the agent could not be detected in mussels obtained from the Pertek region. DNA extracted …

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Determination Of Proximate Composition And Quality Changes In The Common Guitarfish (Rhinobatos Rhinobatos L., 1758) During Cold Storage, Ayşe Bahar Yilmaz, Deni̇z Akpinar Jan 2003

Determination Of Proximate Composition And Quality Changes In The Common Guitarfish (Rhinobatos Rhinobatos L., 1758) During Cold Storage, Ayşe Bahar Yilmaz, Deni̇z Akpinar

Turkish Journal of Veterinary & Animal Sciences

Quality changes in common guitarfish fillets vacuum packed and non-vacuum packed in polyethylene material were investigated during 6 months' cold storage at -18 ^oC. Total yield of the common guitar fish was around 65%. pH, TVB-N, crude protein, moisture, crude ash and fat were 6.50-6.82, 19.87-48.62 mg/100 g, 16.63-22.63%, 75.83-79.88%, 1.00-1.65% and 0.2-0.7%, respectively. Sensory analyses of fillets were carried out at 3 month intervals. The sensory analyses were performed by a trained panel and samples were presented to the panel after baking. The panel scored the samples by checking their appearance, smell, chewiness, aroma, moistness and overall palatability. Consequently …

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Pharmacokinetics Of Oxytetracycline In Rainbow Trout (Oncorhynchus Mykiss) Following Administration Of Medicated Feed, İdri̇s Türel, Gürdal Dağoğlu, Orhan Yilmaz, Ertuğrul Kankaya, Fazil Şen Jan 2003

Pharmacokinetics Of Oxytetracycline In Rainbow Trout (Oncorhynchus Mykiss) Following Administration Of Medicated Feed, İdri̇s Türel, Gürdal Dağoğlu, Orhan Yilmaz, Ertuğrul Kankaya, Fazil Şen

Turkish Journal of Veterinary & Animal Sciences

The pharmacokinetics of oxytetracycline (OTC) were investigated in rainbow trout in freshwater at 10.5 ^oC. A group of 70 fish received OTC, an intravascular injection (i.v.) of 5 mg/kg body weight. Another group of 105 fish were fed medicated feed containing a dose of 100 mg/kg body weight. OTC plasma concentrations were determined by high performance liquid chromatography. The pharmacokinetic parameters were described by a two-compartment open model giving distribution half-lives of 0.907 h and 16.058 h, and elimination half-lives of 33.5 h and 27 h for i.v. and oral administration of OTC, respectively. After i.v. administration of OTC, other …

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Amplification Of The Matrix Gene Of Rbok Vaccine Strain Of Rinderpest Virus (Rpv) By Polymerase Chain Reaction And Cloning Into Topo^R Xl Cloning Vector, Ahmet Kürşat Azkur, Yusuf Bolat, Mehmet Zi̇ya Doymaz Jan 2003

Amplification Of The Matrix Gene Of Rbok Vaccine Strain Of Rinderpest Virus (Rpv) By Polymerase Chain Reaction And Cloning Into Topo^R Xl Cloning Vector, Ahmet Kürşat Azkur, Yusuf Bolat, Mehmet Zi̇ya Doymaz

Turkish Journal of Veterinary & Animal Sciences

In this study, the matrix (M) gene of the RBOK vaccine strain of rinderpest (RPV) was amplified by polymerase chain reaction (PCR) and cloned into TOPO^R XL cloning vector. For this purpose, Vero cells were infected with the RBOK vaccine strain of RPV and total RNA was obtained from the infected cells. cDNA of the matrix gene was obtained by reverse transcription from the total RNA. Amplification of the cDNA with PCR was achieved by using the M gene specific primer and PCR products of the M gene were directly ligated into the TOPO^R XL cloning vector and this recombinant …

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The Use Of Recombinant Hemagglutinine Protein Of Rinderpest Virus In Enzyme Immunoassay, Hakan Bulut, Yusuf Bolat, Yasemi̇n Bulut, Aykut Özdarendeli̇, Mehmet Zi̇ya Doymaz Jan 2003

The Use Of Recombinant Hemagglutinine Protein Of Rinderpest Virus In Enzyme Immunoassay, Hakan Bulut, Yusuf Bolat, Yasemi̇n Bulut, Aykut Özdarendeli̇, Mehmet Zi̇ya Doymaz

Turkish Journal of Veterinary & Animal Sciences

In this study, Rinderpest virus (RPV) recombinant hemagglutinine protein (rH) fused with protein A region of Staphylococcus aureus was expressed in Escherichia coli and purified by IgG affinity chromatography. rH protein was also used to establish enzyme immunoassay. Therefore, to prevent IgG binding to the protein A the wells coated with the rH proteins were blocked by human serum. Afterwards, RPV antigens were added to the wells to evaluate this assay. To this end, serum from mice immunized with RPV was used for EIA. Our data indicated that the rH protein fused with protein A of Staphylococcus aureus can be …

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Adherence And Slime Production In Coagulase Negative Staphylococci Isolated From Different Animal Sources, Oktay Keski̇n, Gülay Altay, Mehmet Akan Jan 2003

Adherence And Slime Production In Coagulase Negative Staphylococci Isolated From Different Animal Sources, Oktay Keski̇n, Gülay Altay, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine slime production and the adherence of coagulase negative staphylococci (CNS) strains isolated from cows' milk with mastitis and chickens which have pathological disorders. For this purpose, 60 CNS strains isolated from cows' milk with mastitis and 60 from chickens suffering from skin and joint diseases were examined. In addition, 20 strains isolated from healthy cows and healthy chickens were used as control groups. Slime production was conductet by the tube method and the results were recorded as -, +, ++ and +++ after 24 h incubation according to the formation of a …

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Some Production Traits Of Karayaka And Bafra (Chios X Karayaka B_1) Sheep, Fati̇h Atasoy, Necmetti̇n Ünal, Hali̇l Akçapinar, Durhasan Mundan Jan 2003

Some Production Traits Of Karayaka And Bafra (Chios X Karayaka B_1) Sheep, Fati̇h Atasoy, Necmetti̇n Ünal, Hali̇l Akçapinar, Durhasan Mundan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate live weight, wool production and some body measurements of Karayaka and Bafra (Chios x Karyaka B_1) sheep. The Karayaka breed is raised in the Black Sea region, while the Sakız (Chios) breed is raised in the coastal areas of western Anatolia. The Bafra type was developed by crossing between Sakız and Karayaka for a female line to use in lamb production. The least squares means obtained for Karayaka and Bafra yearlings were determined to be 48.4 and 55.0 kg for live weight, 56.2 and 63.8 cm for wither height, 59.6 and 71.0 cm …

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Fertility Traits, Survival Rate And Growth Characteristics Of Karayaka And Bafra (Chios X Karayaka B_1) Genotypes, Necmetti̇n Ünal, Fati̇h Atasoy, Hali̇l Akçapinar, Meti̇n Erdoğan Jan 2003

Fertility Traits, Survival Rate And Growth Characteristics Of Karayaka And Bafra (Chios X Karayaka B_1) Genotypes, Necmetti̇n Ünal, Fati̇h Atasoy, Hali̇l Akçapinar, Meti̇n Erdoğan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the fertility traits, survival rate and growth characteristics of Karayaka and Bafra (Chios x Karayaka B_1) genotypes. Karayaka and Bafra genotypes are raised in the Black Sea region of Turkey. In the study, fertility results for Karayaka and Bafra were determined to be 92.3 and 93.7% for birth rates, and 1.08 and 1.78 for litter size, respectively. The survival rates of Karayaka and Bafra lambs at weaning (90 days) were 93.6 and 91.9%, respectively. The least squares means obtained for Karayaka and Bafra lambs were 3.1 and 3.7 kg for birth weight, 19.5 …

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