Veterinary Medicine Commons^™

An Eiav Field Isolate Reveals Much Higher Levels Of Subtype Variability Than Currently Reported For The Equine Lentivirus Family, Jodi K. Craigo, Shannon Barnes, Baoshan Zhang, Sheila J. Cook, Laryssa Howe, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

BACKGROUND: Equine infectious anemia virus (EIAV), a lentivirus that infects horses, has been utilized as an animal model for the study of HIV. Furthermore, the disease associated with the equine lentivirus poses a significant challenge to veterinary medicine around the world. As with all lentiviruses, EIAV has been shown to have a high propensity for genomic sequence and antigenic variation, especially in its envelope (Env) proteins. Recent studies have demonstrated Env variation to be a major determinant of vaccine efficacy, emphasizing the importance of defining natural variation among field isolates of EIAV. To date, however, published EIAV sequences have been …

Evaluation Of The Cervidtb Stat-Pak For The Detection Of Mycobacterium Bovis Infection In Wild Deer In Great Britain, S. Gowtage-Sequeira, A. Paterson, K. P. Lyashchenko, S. Lesellier, M. A. Chambers

Michigan Bovine Tuberculosis Bibliography and Database

Deer are acknowledged as hosts of Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), and determining the prevalence of infection in deer species is one of the key steps in understanding the epidemiological role played by cervids in the transmission and maintenance of bTB in the United Kingdom. This study evaluated a rapid lateral-flow test for the detection of bTB in samples from wild deer species in the United Kingdom. Fallow deer (Dama dama), roe deer (Capreolus capreolus), and red deer (Cervus elaphus) from areas in Wales, the Cotswolds, and southwestern England …

South Dakota Thriving, Fall 2009, College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

Table of Contents:
[Page] 2 Growing Farmers Markets: South Dakota Cooperative Extension Service Helps Develop and Grow Booming Homegrown markets
[Page] 4 Lighari Leads Restructuring of Extension: Director of South Dakota Cooperative Extension Service Focuses on Building Relationships
[Page} 6 South Dakota's Newest Research Station: The Addition of the SDSU Cow Camp Means Another Tremendous Resource for South Dakota and SDSU
[Page] 8 SDSU Rodeo Builds World Champs: Focus on Academics and Team Leads to Success Inside Both the Classroom and the Arena
|[Page] 12 Behind the Scenes in SDSU's Anatomy Lab: SDSU's Hands-On Anatomy Lab Gives Students the Edge …

Transcriptional Profiling Differences For Articular Cartilage And Repair Tissue In Equine Joint Surface Lesions, Michael J. Mienaltowski, Liping Huang, David D. Frisbie, C. Wayne Mcilwraith, Arnold J. Stromberg, Arne C. Bathke, James N. Macleod

Veterinary Science Faculty Publications

BACKGROUND: Full-thickness articular cartilage lesions that reach to the subchondral bone yet are restricted to the chondral compartment usually fill with a fibrocartilage-like repair tissue which is structurally and biomechanically compromised relative to normal articular cartilage. The objective of this study was to evaluate transcriptional differences between chondrocytes of normal articular cartilage and repair tissue cells four months post-microfracture.

METHODS: Bilateral one-cm2 full-thickness defects were made in the articular surface of both distal femurs of four adult horses followed by subchondral microfracture. Four months postoperatively, repair tissue from the lesion site and grossly normal articular cartilage from within the same …

Proliferation And Apoptosis Of The Bitch Ovary During The Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Sukanya Manee-In, Promporn Raksaseri, Kongkiat Srisuwatanasagul

The Thai Journal of Veterinary Medicine

In order to maintain homeostasis of the ovary, proliferation and apoptosis occur simultaneously in different ovarian cell types. It was shown that steroid hormone which changed during the oestrous cycle, may regulate these mechanisms in normal mammal ovarian tissues. Therefore, the present study was aimed to study cell proliferation and apoptosis during different stages of the oestrous cycle in the bitch ovary by using immunohistochemistry and TUNEL assay. The bitch ovaries were collected at 4 different stages of the oestrous cycle, which were prooestrus, oestrus, dioestrus and anoestrus. Immunohistochemistry and TUNEL assay were applied to detect Ki-67 protein and apoptosis …

Effects Of Medium Chain Fatty Acids, Organic Acids And Fructo-Oligosaccharide On Cecal Salmonella Enteritidis Colonization And Intestinal Parameters Of Broilers, Sucheera Chotikatum, Indhira Kramomthong, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

Medium chain fatty acids (MCA) are one of new additives used to control Salmonella infection and it is interesting to examine their physiological role in chickens comparing with other additives. The objective of this investigation was to study the effect of MCA, mixed organic acids (ORA) and fructo-oligosaccharide (FOS) on cecal Salmonella enterica serovar Enteritidis colonization and physiological changes of intestine in broilers. Six hundred, day old, male and female broiler chicks were allocated into 4 treatments. The treatments were CON: basal corn-soybean meal diet, FOS: basal diet supplemented with 4 g/kg FOS, ORA: basal diet plus water supplemented with …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Atp Release By Infected Bovine Monocytes Increases The Intracellular Survival Of Mycobacterium Avium Subsp. Paratuberculosis, Seng-Ryong Woo, Raul G. Barletta, Charles Czuprynski

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne’s disease, a chronic intestinal infection in ruminants. Adenosine 5′-Triphosphate (ATP) has been reported to induce killing of several Mycobacterium species in human and murine macrophages. We investigated whether ATP secreted from M. avium subsp. paratuberculosis-infected bovine monocytes affects intracellular survival of the bacilli. Bovine monocytes constitutively secreted ATP during an 8-day incubation period in vitro; however, M. avium subsp. paratuberculosis infection did not enhance ATP release. Removal of extracellular ATP by the addition of apyrase increased the viability of infected monocytes, but surprisingly decreased the number of viable intracellular …

Successful Transcervical Insemination In Giant Panda At Chiangmai Zoo, Thailand, Boripat Siriaroonrat, Ampika Thongphakdee, Wanlaya Tipkantha, Kannikar Nimtragul, Kornchai Kornkeawrat, Ratchaneewan Khejornwong, Prasertsak Buntragulpoontawee, Sumate Kamolnorranath

The Thai Journal of Veterinary Medicine

No abstract provided.

Penaeid Shrimp Immune System, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Angel Isidro Campa-Cordova, Antonio Luna-Gonzalez, Felipe Ascencio

The Thai Journal of Veterinary Medicine

Research on an innate immune system of penaeid shrimp is greatly motivated by economical requirements, because their culture is limited by the development of infectious diseases. As invertebrates, shrimp’s natural immunity acts as a fast and efficient defence mechanism against the pathogens. Their immune system involve hemocytes (for encapsulation, nodule formation and phagocytosis), several plasma components (antimicrobial peptides, histones, lysosomal enzymes, lipopolysaccharide, β-1,3-glucan binding proteins, and recognition molecules), and multimeric systems (clotting protein cascade, prophenoloxidase system). When these defense mechanisms fail to protect the shrimp against bacteria, viruses, fungi, protozoa and their products, disease develops and a negative impact takes …

In Vitro Antibiotic Susceptibility Of Aeromonas Hydrophila Isolated From Disease Ornamental Fish, Malinee Jongjareanjai, Nongnut Assawawongkasem, Nantarika Chansue

The Thai Journal of Veterinary Medicine

Thirty bacterial isolates were derived from ornamental fish patients (n=30) of the Veterinary Medical Aquatic Animal Research Center (VMARC), Faculty of Veterinary Science, Chulalongkorn University. The isolated bacterial strains were identified by using commercial biochemical identification kit (API Kits, bioMerieux® SA France). The antibiotics susceptibility testing was performed by disc diffusion method, using 24 types of antibiotic discs (OXOID, Oxoid Ltd, UK). The result showed that the majority of the isolated bacteria was Aeromonas hydrophila (27/30). In the A. hydrophila susceptibility study, 66.30% of bacteria were antibiotic resistant, 9.35% were intermediately sensitive and 24.35% were sensitive strains against the tested …

Comparative Efficacy Of Enrofloxacin And Oxytetracycline By Different Administration Methods In Broilers After Experimental Infection With Avian Pathogenic Escherichia Coli, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The aims of this study were to compare the efficacy of 2 kinds of antibiotics that are commonly used in the Thai broiler industry and to determine the optimal times of drug administration. One hundred and sixty, 21-day-old broilers were divided into eight groups. Chickens in groups 1, 2 and 3 were challenged with avian pathogenic E. coli (APEC) and received oxytetracycline for the first 3 days after infection via oral, drinking water within 2 h and drinking water throughout the day, respectively. The chickens in groups 4, 5 and 6 were challenged with APEC and received enrofloxacin for the …

The Indicative Influence Of Oxidative Stress On Low Milk Yields In Dairy Cattle, Witaya Suriyasathaporn, Usanee Vinitketkumnuen, Teera Chewonarin, Vena Chupia, Tanu Pinyopummintr

The Thai Journal of Veterinary Medicine

The goal of this study was to identify the correlation between milk yield and milk malondialdehyde (MDA), an oxidative stress marker. The study was conducted using cows from eight small-holder dairy farms during October to December 2004. The data comprised of sample date, milk yield, and the recent calving date were recorded. Morning milk samples were collected monthly for the measurement of somatic cell counts (SCC) and MDA level using the automate counter and the modified Smith’s method, respectively. The final data set included 131 milk data from 74 cows. Overall means and SEM of milk yields, somatic cell scores …

Efficacy Of Microsatellite Markers In Parentage Control In Swine, Wanwisa Yaemmeeklin, Jutarat Jirasupphachok, Weerapong Koykul, Duangsmorn Suwattana

The Thai Journal of Veterinary Medicine

The efficacy of microsatellite markers was evaluated in order to use in parentage control in swine. Genomic DNA from 80 samples were extracted, and amplified using 16 microsatellite markers (D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, S0766, SJ859, SJ923, SJ924, SJ925, SJ926, SJ927, SJ929, X53085 and X63893) in each single polymerase chain reaction (PCR). The PCR products were analyzed using agarose and polyacrylamide gel electrophoresis (PAGE). The result showed that 15 microsatellite loci could be amplified except SJ925. Seven suitable microsatellite markers were selected for parentage control, including D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, and S0766. Allelic numbers of the selected …

Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa

The Thai Journal of Veterinary Medicine

This study was to conduct a surveillance of Mycoplasma synoviae (MS) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1-4.5 months in the area of Nakornpathom province in the September 2005-October 2006 period. Each bird was bled for MS serology by serum plate agglutination (SPA) and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MS antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA and PCR procedure were 12, 9 and 8 flocks, …

Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai

The Thai Journal of Veterinary Medicine

Mastitis is a most frequent and costly disease of dairy cattle worldwide. All three contagious mastitis pathogens, Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were investigated in 55 bulk tank milk samples from dairy cattle herds in Khon Kaen Province Thailand, by nested PCR and/or conventional bacterial culture. Bulk milk somatic cell count (BMSCC) was used as indicator for mastitis problem; i.e. > 5x105 somatic cells/ml of milk. The prevalence of Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were 1.8%, 21.8% and 7.3%, respectively. Results from BMSCC indicated mastitis problem in 47 herds. However, 34 high-BMSCC with negative-contagious pathogen identification samples …

Growth Characteristics Of The H5n1 Avian Influenza Virus In Chicken Embryonic Eggs And Mdck Cells, Wisanu Wanasawaeng, Napawan Bunpapong, Wichet Leelamanit, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Chicken embryonic eggs and Madin-Darby canine kidney (MDCK) cell line were used for comparing the propagation of avian influenza A (H5N1) virus (C2105Dx1, a Thai isolate). The growth of the H5N1 virus was determined by using HA test and 50% infectivity dose to assess the suitability of the systems supporting the propagation of the virus. The results indicated that the Thai H5N1 virus was propagated better in the chicken embryonic eggs, which should be considered as a system of choice for the avian H5N1 virus isolation. The optimal time for harvesting the selected Thai H5N1 virus was at 24 hours …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Veterinary Ethics And Production Diseases, Bernard E. Rollin

Professional Veterinary Ethics Collection

An animal's welfare should be governed by five freedoms, namely, freedom from hunger and thirst, freedom from discomfort, freedom from pain, injury or disease, freedom to express normal behavior and freedom from fear and distress. If the essence of veterinary medicine is to act like a physician for animals then the profession must be vocal in opposition to production diseases, which can be prevented by changing the system of production.

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

[Page] 1- Early Spring Cow Losses in Southwest South Dakota Director's Message [Page] 2-The Cost of a Veterinary Medical Education [Page] 4- Molecular Diagnostics: The “PCR” Lab at ADRDL Pieces and Parts [Page] 6- Rabies Submission Reminder [Page] 6- ADRDL Hosts Heartland Veterinary Microbiology Annual Meeting [Page] 6- Veterinary Science Graduate Student wins CRWAD Award [Page] 6- Dr. Eric Nelson Receives Gamma Sigma Delta Research Award [Page] 6- New Tenure Track Faculty Positions Filled in Veterinary Science Department [Page] 6- Rusk Named SDSU Animal and Range Sciences Department Head [Page] 7- ADRDL Influenza Diagnostics in the Era of “Novel” H1N1 …

A New Approach For Managing Bovine Tuberculosis: Veterinary Services’ Proposed Action Plan

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (TB) is a serious disease with animal health, public health, and international trade consequences. The cooperative Federal-State-industry effort to eradicate bovine TB from cattle in the United States has made significant progress since the program’s inception in 1917. However, the goal of eradication remains elusive.

This proposed action plan presents Veterinary Services’ (VS’) current thinking about changes we are considering for the TB program to address our current challenges.

This action plan will:
1. Reduce the introduction of TB into the U.S. national herd from imported animals and wildlife by:
o Applying additional requirements to cattle imports from …

Gp3 Is A Structural Component Of The Prrsv Type Ii (Us) Virion, M. De Lima, I. H. Ansari, P. B. Das, B. J. Ku, F. J. Martinez-Lobo, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Abstract Glycoprotein 3 (GP3) is a highly glycosylated PRRSV envelope protein which has been reported as being present in the virions of PRRSV type I, while missing in the type II PRRSV (US) virions. We herein present evidence that GP3 is indeed incorporated in the virus particles of a North American strain of PRRSV (FL12), at a density that is consistent with the minor structural role assigned to GP3 in members of the Arterivirus genus. Two 15aa peptides corresponding to two different immunodominant linear epitopes of GP3 derived from the North American strain of PRRSV (FL12) were used as antigen …

Comparison Of The Efficacy Of The Immune Complex And Conventionally Live Vaccine In Broilers Against Infectious Bursal Disease Infection, Niwat Chansiripornchai, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

One hundred and thirty two, one day old broiler chickens were divided into 4 groups. Group 1 (1 day old) was subcutaneously vaccinated with immune complex vaccine into the nape of the neck. Group 2 and 3 (14 days old) were vaccinated with different commercial products, intermediate-plus strains of live attenuated infectious bursal disease (IBD) vaccine I and II, respectively, via the oral route. Group 4 acted as the positive control. Chickens were bled at days 1, 14, 28 and 38 for evaluating IBD virus antibody titers by ELISA. The body weight, FCR, bursa to body weight ratios and bursal …

Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong

The Thai Journal of Veterinary Medicine

The present study aimed to examine the general morphology of Thai swamp buffalo’s oviducts by light microscopy (LM) and scanning electron microscopy (SEM) as well as the expression of estrogen receptor alpha (ERα) and progesterone receptor isoform B (PRB) by immunohistochemical technique at the prepubertal stage. The samples composed of the uterotubal junction (UTJ), isthmus, ampulla and infundibulum of prepubertal Thai swamp buffalo oviducts (n=6) were collected from a local slaughterhouse. The results showed that the epithelium of prepubertal Thai swamp buffalo oviducts was composed of ciliated and secretory cells but the feature of nuclear/cytoplasmic protrusions of secretory cells was …

Efficacy Of A Chimeric Pcv2 Vaccine: A Field Trial, Termsitthi Paphavasit, Philip Lehrbach, Wichian Navasakuljinda, Roongtham Kedkovid, Sitthichok Lacharoje, Roongroje Thanawongnuwech, Komkrich Teankum

The Thai Journal of Veterinary Medicine

Field trial of a Chimeric porcine circovirus type 2 (PCV2) (Suvaxyn PCV2®, Fort Dodge Animal Health, USA) vaccine was performed in order to investigate the efficacy on decreasing pathological lesions and PCV2 viremia in a PCV2-affected swine herd in Thailand. A herd was selected based on history and serological data. Three-week-old pigs (n = 200) were equally divided into two groups. At 4 weeks old, the pigs (n = 100) were injected intramuscularly with 2 ml of Suvaxyn PCV2® vaccine and the non-vaccinated pigs were injected with 2 ml of normal saline. Sera were collected from 20 pigs per group …

Cryopreservation Of Immature Porcine Oocytes Using Open Pulled Straw Vitrification, Theerawat Tharasanit, Sasitorn Rungarunlert, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The aims of this study were to investigate the meiotic competence of immature porcine oocytes following open pulled straw (OPS) vitrification and warming and also to test the efficiency of the pretreatment of immature oocytes with CB prior to OPS vitrification. Experiment 1, cumulus oocyte complexes (COCs) were randomly assigned to OPS vitrification, cryoprotectant (CPA) toxicity test and non-CPA/non-vitrified controls. Experiment 2, the COCs were treated with 7.5 μg/ml CB for 30 min. The oocytes were either immediately fixed or further incubated in a CB-free medium for 5 h, non-CB treated COCs served as controls. Experiment 3, CB-pretreated COCs were …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.