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Articles 5221 - 5250 of 9777

Full-Text Articles in Veterinary Medicine

Effect Of A Simulate Show Jumping Competition On The Blood Gas Profile Of Horses Trained For Show Jumping, Francesco Fazio, Vanessa Messina, Stefania Casella, Claudia Giannetto, Simona Marafioti, Giuseppe Piccione Jan 2012

Effect Of A Simulate Show Jumping Competition On The Blood Gas Profile Of Horses Trained For Show Jumping, Francesco Fazio, Vanessa Messina, Stefania Casella, Claudia Giannetto, Simona Marafioti, Giuseppe Piccione

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to evaluate the changes in the blood gas profile of jumpers after warm up and after a show jumping competition. For this purpose, 10 Sella Italiana geldings, 7 years old and clinically healthy, were used. The horses were trained to take part in show jumping competitions. Blood samples were collected at rest, after the warm up, immediately after the exercise, and 45 min after the end of exercise. Standard bicarbonate concentration (SBC), hydrogen carbonate ion (HCO_3^-), total carbon dioxide (TCO_2), oxygen capacity (O_2Cap), oxygen content (O_2Ct), base excess of the blood (BE-b), base …


The Effect Of Oxytocin And Cloprostenol Application Via Umbilical Artery Immediately After Dystocia On Time And Rate Of Fetal Membrane Removal In Cows, Yaşar Akar, Ömer Kizil, Nevzat Saat, Murat Yüksel Jan 2012

The Effect Of Oxytocin And Cloprostenol Application Via Umbilical Artery Immediately After Dystocia On Time And Rate Of Fetal Membrane Removal In Cows, Yaşar Akar, Ömer Kizil, Nevzat Saat, Murat Yüksel

Turkish Journal of Veterinary & Animal Sciences

The aim of this clinical study was to investigate the effects of oxytocin and cloprostenol application via umbilical artery on the time and rate of fetal membrane removal in cows with dystocia immediately after parturition. Metabolic profiles indicated certain serum biochemical parameters that were also determined in the cows. The study was performed on 60 cows with dystocia. All of the cows were randomly divided into 3 equal subgroups. The first group was injected with 100 IU oxytocin, while the second group was treated with 0.15 mg cloprostenol and the third group (the control group) received 10 mL 0.9% NaCl …


The Influence Of Egg Shell Crack Types On Hatchability And Chick Quality, Mozhdeh Moosanezhad Khabisi, Ahmad Salahi, Seyed Naser Mousavi Jan 2012

The Influence Of Egg Shell Crack Types On Hatchability And Chick Quality, Mozhdeh Moosanezhad Khabisi, Ahmad Salahi, Seyed Naser Mousavi

Turkish Journal of Veterinary & Animal Sciences

Fertile eggs were obtained from a commercial flock of Ross broiler breeders (51 weeks old) and candled to determine the presence of hairline or star cracks. Eggs (5400 total) were assigned in equal numbers to 3 treatments (hairline-cracked, star-cracked, and normal eggs) and incubated for 21 days. Upon hatching, 10 chicks per treatment were euthanized, and organ weights were measured. Compared with normal eggs, the presence of egg shell cracks (regardless of type) resulted in higher egg weight loss at the time of transfer; a decrease in hatchability, hatch of fertile eggs, chick length, yolk-free body mass (P < 0.01), and percentage of hatchling breast and liver weight (P < 0.05); and an increase in the contamination rate of eggs (P < 0.01). Weight loss in hairline-cracked eggs was significantly higher than in star-cracked eggs (P < 0.001). Percentage hatchability and body weight of chicks hatched from star-cracked eggs was higher than in chicks from hairline-cracked eggs (P < 0.001). Crack type did not have a significant effect on chick length and body weight uniformity; however, the Pasgar score as a quality indicator was higher in chicks hatched from star-cracked eggs than those from hairline-cracked eggs (P < 0.05). There were no significant differences among treatments in terms of heart and small intestine weight. The contamination rate of hairline-cracked eggs was higher than that of star-cracked (P < 0.01). A higher incidence of embryonic mortality was observed during days 1-8 of incubation for shell-cracked eggs (P < 0.001). Total embryonic mortality in hairline-cracked, star-cracked, and normal eggs was 51.1%, 36.6%, and 13.3%, respectively. This study showed that egg shell cracks reduced incubation parameters and chick quality. The negative effects of hairline cracks were more pronounced than those of star cracks.


Immunopotency Of Novel Oil Adjuvant Vaccines Employing Pasteurella Multocida Biofilm And Capsule Enhanced Organisms In Ducklings, Ramachandranpillai Rajagopal, Govindapillai Krishnan Nair, Mini Mangattumuruppel, Joseph Leo, Saseendranath Raghavan Mapranath Jan 2012

Immunopotency Of Novel Oil Adjuvant Vaccines Employing Pasteurella Multocida Biofilm And Capsule Enhanced Organisms In Ducklings, Ramachandranpillai Rajagopal, Govindapillai Krishnan Nair, Mini Mangattumuruppel, Joseph Leo, Saseendranath Raghavan Mapranath

Turkish Journal of Veterinary & Animal Sciences

Pasteurella multocida-a gram-negative bacterium with a broad host range and ubiquitous distribution—is a serious problem in ducks, and the best method of control is vaccination with locally prevalent strains. The present study was conducted to compare the immunopotency of vaccines employing P. multocida biofilms and capsule enhanced organisms with ordinary broth grown organisms in 1-month-old ducklings, by measuring the humoral immune response and the protection conferred by each vaccine during homologous challenge with virulent organisms. Formalin inactivated oil adjuvant bacterin vaccines were prepared from Pasteurella multocida A: 1. They were grown in conventional broth, capsule enhancement medium, and under biofilm …


An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng Jan 2012

An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; …


Effects Of Inulin And \Beta-Glucan Supplementation In Broiler Diets On Growth Performance, Serum Cholesterol, Intestinal Length, And Immune System, Asia Sanhouri Elrayeh, Gülteki̇n Yildiz Jan 2012

Effects Of Inulin And \Beta-Glucan Supplementation In Broiler Diets On Growth Performance, Serum Cholesterol, Intestinal Length, And Immune System, Asia Sanhouri Elrayeh, Gülteki̇n Yildiz

Turkish Journal of Veterinary & Animal Sciences

This study was designed to investigate the effects of a supplement of inulin and \beta-glucan into broiler rations on growth performance, serum cholesterol, intestinal length, and immune system. A total of 320 male 1-day-old Ross PM3 broiler chicks were divided into 1 control and 3 treatment groups, each comprising 80 birds. Each group was divided into 4 replicate groups of 20 birds each. The treatment groups were supplemented with 0.7% inulin, 0.014% \beta-glucan, and 0.7% inulin + 0.014% \beta-glucan, respectively. The experiment lasted for 6 weeks. Immunomodulatory roles were also investigated by measuring the effects on specific antibody titer, and, …


Protective Effect Of N-Acetyl-L-Cysteine Against Acrylamide-Induced Oxidative Stress In Rats, Ebru Işik Alturfan, Ayfer Beceren, Ahmet Özer Şehi̇rli̇, Zeynep Emel Demi̇ralp, Göksel Şener, Gülden Zehra Omurtag Jan 2012

Protective Effect Of N-Acetyl-L-Cysteine Against Acrylamide-Induced Oxidative Stress In Rats, Ebru Işik Alturfan, Ayfer Beceren, Ahmet Özer Şehi̇rli̇, Zeynep Emel Demi̇ralp, Göksel Şener, Gülden Zehra Omurtag

Turkish Journal of Veterinary & Animal Sciences

Acrylamide (AA), used in many fields, from industrial manufacturing to laboratory work, is also formed during the heating process through the interactions of amino acids. Therefore, AA poses a significant risk for both human and animal health. This study aimed to elucidate whether N-acetyl-L-cysteine (NAC) treatment could modulate AA-induced oxidative changes in the brain, lung, liver, kidney, and testes tissues of the rat. Rats were divided into 4 groups, as the control (C), NAC [150 mg/kg intraperitoneally (i.p.)], AA (40 mg/kg i.p.), and NAC + AA groups. After 10 days, the rats were decapitated and the tissues were excised. Malondialdehyde …


Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci Jan 2012

Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci

Turkish Journal of Veterinary & Animal Sciences

Neuronal and extraneuronal lipofuscinosis was determined in Merino sheep (6 sheep and 3 heads of sheep) of 2-3 years old that had grazed on Asphodelus aestivus seeds. Clinically, the sheep exhibited tremors, paresis, oral and nasal discharge, and severe respiratory distress. Haemorrhagic necrotic tracheitis, aspiration pneumonia, and subacute enteritis were observed in 6 sheep during macroscopic examinations. The brains of the animals were of firm consistency and yellowish brown in colour. Histological examination of the central nervous system revealed that most of the cytoplasm of neurons contained yellowish brown pigment granules. Pigmentations were particularly detected in the pons, medulla oblongata, …


Changes In Cell Wall Compositions And Degradation Kinetics Of Electron Beam-Irradiated Sugarcane Bagasse, Parvin Shawrang, Abbas Majdabadi, Ali Asghar Sadeghi Jan 2012

Changes In Cell Wall Compositions And Degradation Kinetics Of Electron Beam-Irradiated Sugarcane Bagasse, Parvin Shawrang, Abbas Majdabadi, Ali Asghar Sadeghi

Turkish Journal of Veterinary & Animal Sciences

Electron beam irradiation of sugarcane bagasse at doses of 250 and 500 kGy could change its cell wall composition and ruminal fiber degradation characteristics by breaking off the lignocellulosic bonds. Nylon bags of untreated or electron beam-irradiated bagasse were suspended in the rumen of 3 fistulated rams for up to 72 h, and the resulting data were fitted to a nonlinear degradation model to calculate the degradation parameters of dry matter (DM) and neutral detergent fiber (NDF). Irradiation had no effect on crude protein, ether extract, and ash, but resulted in a linear decrease of the cell wall contents. An …


Prevalence And Factors Affecting The Presence Of Campylobacter Spp. In Broiler Carcasses In Bulgaria, Hristo Daskalov, Alexander Maramski Jan 2012

Prevalence And Factors Affecting The Presence Of Campylobacter Spp. In Broiler Carcasses In Bulgaria, Hristo Daskalov, Alexander Maramski

Turkish Journal of Veterinary & Animal Sciences

In an 11-month study, 292 samples from 13 slaughterhouses in Bulgaria were investigated for the presence of Campylobacter spp., and some factors affecting Campylobacter prevalence were analyzed. The study revealed that 44.9% of the samples were positive for Campylobacter spp. The main species detected were C. coli and C. jejuni. There was also one confirmed C. lari isolate. The C. coli strains prevailed over C. jejuni at 61.8% and 37.4%, respectively. A seasonal variation in the Campylobacter spp. presence in slaughter broiler carcasses was found with a predominance of positive isolates during June and July 2008. The conventional type of …


Fecal Progesterone Analysis For Monitoring Reproductive Status In Dairy Goats, Amelia Miranda Morgiana Jack, Chao-Chin Chang, Huo-Cheng Peh, Jacky Peng-Wen Chan Jan 2012

Fecal Progesterone Analysis For Monitoring Reproductive Status In Dairy Goats, Amelia Miranda Morgiana Jack, Chao-Chin Chang, Huo-Cheng Peh, Jacky Peng-Wen Chan

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to evaluate the use of fecal progesterone (FP_4) concentration for early pregnancy diagnosis, estrus detection, and predicting the litter size and parturition date in dairy goats. Fecal samples were collected from 17 hand-mated goats 3 times a week for 22-23 weeks, beginning on the day of mating. The levels of FP_4 were determined by enzyme-linked immunosorbent assay (ELISA). Significant positive correlations were found between the levels of serum P_4 and FP_4 (r = 0.8787, n = 13). Pregnancy diagnosis was performed at 2 months postmating with transabdominal ultrasonography and confirmed upon birth of the offspring. …


Determination Of Egg Consumption And Consumer Habits In Turkey, Cengi̇zhan Mizrak, İsmai̇l Durmuş, Serdar Kamanli, Şahnur Erdoğan Demi̇rtaş, Süleyman Kalebaşi, Ender Karademi̇r, Murat Doğu Jan 2012

Determination Of Egg Consumption And Consumer Habits In Turkey, Cengi̇zhan Mizrak, İsmai̇l Durmuş, Serdar Kamanli, Şahnur Erdoğan Demi̇rtaş, Süleyman Kalebaşi, Ender Karademi̇r, Murat Doğu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine egg consumption habits in Turkey. Questionnaires prepared to determine habits regarding egg consumption were supplied to 2241 families. Sampling sizes and sample addresses were provided by the Turkish Statistical Institute. Data obtained through the questionnaires were analyzed using SPSS. Of the families that participated in the study, 98% consume eggs and the number of eggs consumed per person was 158 per year. In addition, 67.82% of the eggs consumed were purchased from supermarkets and 62.40% of the consumers pay most of their attention to the production date. The eggs were most often …


A Note On Phthirapteran Ectoparasites Infesting Yellow-Legged Green Pigeon Treron Phoenicoptera (Columbiformes: Columbidae), Aftab Ahmad, Gaurav Arya, Nayanci Bansal, Oldrich Sychra, Arun Kumar Saxena Jan 2012

A Note On Phthirapteran Ectoparasites Infesting Yellow-Legged Green Pigeon Treron Phoenicoptera (Columbiformes: Columbidae), Aftab Ahmad, Gaurav Arya, Nayanci Bansal, Oldrich Sychra, Arun Kumar Saxena

Turkish Journal of Veterinary & Animal Sciences

The present report records the simultaneous occurrence of ischnoceran lice Columbicola phoenicopterae and Coloceras setosum on the Yellow-legged Green Pigeon in India. The prevalence, intensity of infestation, range of infestation, and nature of the antennal sensilla of these 2 species on 12 of these pigeons have also been recorded.


Protective Effects Of Salvia Officinalis Extract Against Cyclophosphamide-Induced Genotoxicity And Oxidative Stress In Rats, Fulya Üstün Alkan, Feraye Esen Gürsel, Ati̇la Ateş, Mustafa Özyürek, Kubi̇lay Güçlü, Mehmet Altun Jan 2012

Protective Effects Of Salvia Officinalis Extract Against Cyclophosphamide-Induced Genotoxicity And Oxidative Stress In Rats, Fulya Üstün Alkan, Feraye Esen Gürsel, Ati̇la Ateş, Mustafa Özyürek, Kubi̇lay Güçlü, Mehmet Altun

Turkish Journal of Veterinary & Animal Sciences

Medicinal plants, especially rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Salvia officinalis L., an aromatic and medicinal plant, is widely used in folk medicine and is well known for its antioxidant properties. Therefore, the present study was designed to investigate the antioxidative, possible genotoxic, and antigenotoxic potency of S. officinalis extract against cyclophosphamide (CYP)-induced oxidative stress and genotoxicity in Wistar albino rats. Animals were orally dosed with S. officinalis extract (50, 100, and 150 mg/kg body weight) for 7 days before the administration of a single intraperitoneal dose of CYP (40 mg/kg …


Ultrasonographic Evaluation Of Cricoarytenoideus Lateralis Muscle In Standardbred Horse, Fulvio Laus, Emanuele Paggi, Matteo Cerquetella, Beniamino Tesei Jan 2012

Ultrasonographic Evaluation Of Cricoarytenoideus Lateralis Muscle In Standardbred Horse, Fulvio Laus, Emanuele Paggi, Matteo Cerquetella, Beniamino Tesei

Turkish Journal of Veterinary & Animal Sciences

The cricoarytenoideus lateralis muscle (CALM) is an adductor of the arytenoid cartilages but is not directly involved in the pathogenesis of recurrent laryngeal neuropathy (RLN) as the abductor cricoarytenoideus dorsalis muscle (CADM) is. Nevertheless, the CALM is the first muscle to experience a neurogenic atrophy in the course of RLN and this feature explains its potential importance in RLN diagnosis. On 30 gelding and 30 female Standardbred horses weighing about 500 kg and aged between 3 and 9 years, an ultrasound examination of the larynx was performed in order to assess shape, size, and echogenicity of the CALMs. The mean …


Establishment And Biological Research Of The Jining Grey Goat Fibroblast Line, Chunyu Bai, Dianjun Wang, Xiaohua Su, Minghai Zhang, Weijun Guan, Yuehui Ma Jan 2012

Establishment And Biological Research Of The Jining Grey Goat Fibroblast Line, Chunyu Bai, Dianjun Wang, Xiaohua Su, Minghai Zhang, Weijun Guan, Yuehui Ma

Turkish Journal of Veterinary & Animal Sciences

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes …


Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang Jan 2012

Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang

Turkish Journal of Veterinary & Animal Sciences

The main objectives of this study were to determine the prevalence and antimicrobial resistance patterns of Escherichia coli isolates in feces of pigs and their production environments on farms. A total of 560 isolates of Escherichia coli, from 112 samples obtained from weaner and finisher pigs, feed, water, and house sparrows on 8 farrow-to-finish farms, were tested for susceptibility to 16 antimicrobials. The prevalence of resistance varied widely (0.0% to 69.5%) among the antimicrobials tested. From the tested swines, 75.75% of the isolates were resistant to one or more antimicrobials. Resistance was significantly more frequent (P = 0.005, P < 0.01) in swine isolates compared to those from feed, water, and sparrows and significantly more frequent (P = 0.007, P < 0.01) in weaner pigs compared to finisher pigs. The findings indicate that resistance to a broad range of antimicrobials was prevalent among fecal E. coli isolates of swine on the study farms. Fortunately, E. coli was not isolated from the feed, water, and house sparrows.


Comparison Of Different Lactation Curve Models In Sahiwal Cows, Vilas Dongre, Ravindar Singh Gandhi, Avtar Singh Jan 2012

Comparison Of Different Lactation Curve Models In Sahiwal Cows, Vilas Dongre, Ravindar Singh Gandhi, Avtar Singh

Turkish Journal of Veterinary & Animal Sciences

The present investigation was carried out on 12,859 fortnightly test-day milk yield records of first lactation pertaining to 643 Sahiwal cows spread over a period of 49 years (1961-2009), maintained at the National Dairy Research Institute, Karnal, India. The comparison of 3 lactation curve models, i.e. the quadratic model, gamma-type function, and mixed log function, was done using fortnightly test-day milk yield records. The mixed log function was the best fit, with the highest (88.75%) coefficient of determination (R2 value) and the lowest (0.08 kg) root mean square error (RMSE) value, whereas the lowest (56.93%) R2 value was observed in …


Prevention Of Predicted Neonatal Isoerythrolysis With Jaundice Foal Agglutination Test In A Newborn Foal, Aykut Göktürk Üner, Güney Kaya, Aydin Oltu Jan 2012

Prevention Of Predicted Neonatal Isoerythrolysis With Jaundice Foal Agglutination Test In A Newborn Foal, Aykut Göktürk Üner, Güney Kaya, Aydin Oltu

Turkish Journal of Veterinary & Animal Sciences

This case report aims to highlight the utility of the jaundice foal agglutination (JFA) test used in the prevention of neonatal foal death caused by equine neonatal isoerythrolysis. The colostrum and the serum of a Friesian mare with repeated neonatal foal deaths and the whole blood of her newborn foal were tested for 5 days with the JFA test to detect a probable incompatibility. For the first 2 days, the JFA test gave a significantly positive reaction, indicating the existence of antibodies in the colostrum of the mare against the foal's erythrocyte antigens. The foal was not allowed access to …


Evaluation Of Elevated Bait Trays For Attracting Blackbirds (Icteridae) In Central North Dakota, George M. Linz, Jamison B. Winter, William J. Bleier Jan 2012

Evaluation Of Elevated Bait Trays For Attracting Blackbirds (Icteridae) In Central North Dakota, George M. Linz, Jamison B. Winter, William J. Bleier

United States Department of Agriculture Wildlife Services: Staff Publications

Sunflower (Helianthus annuus L.) became an economically important crop in North Dakota in the 1970s, providing a major source of food for post-breeding blackbirds (Icteridae). Reducing local blackbird populations with rice grains treated with an avicide is one proposed alternative for reducing sunflower damage. In fall 2007 and 2008, we evaluated the idea of attracting blackbirds to rice-baited trays attached to wire cages supplied with live blackbirds. During our observations (1011 h), we saw 3888 birds, consisting of 25 species and 12 families, on the bait trays. Blackbirds made up 90.4% of the bird observations, whereas sparrows (Emberizidae) made …


Koutango: Under Reported Arboviral Disease In West Africa, Jaime Matias De Araujo Lobo Jan 2012

Koutango: Under Reported Arboviral Disease In West Africa, Jaime Matias De Araujo Lobo

LSU Doctoral Dissertations

Arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illnesses worldwide. As crucially important emerging pathogens, they have caused multiple, notable epidemics of human disease and unnoticed epizootics over recent decades. Despite the public health relevance, very little is known about the geographic distribution of the agents and vectors, relative impact, and risk factors associated to the arboviral infection in many regions of the world and in the tropics in particular. Presented in this dissertation is an experimental study that explores the serology screening of serum samples from 151 patients whom were diagnosed with undifferentiated febrile illness …


Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury Jan 2012

Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury

LSU Doctoral Dissertations

Herpes simplex virus type I (HSV-1) is a neurotropic virus that infects primarily mucocutaneous epithelial cells and nervous tissue. Membrane fusion is an important aspect of the HSV-1 lifecycle, that occurs during viral entry (virus-cell fusion), viral spread (cell-to-cell fusion), as well as, during virion morphogenesis (assembly and egress). These membrane fusion steps involve complex interactions between multiple viral glycoproteins and cellular receptors. HSV-1 glycoprotein B (gB) is necessary but not sufficient for membrane fusion events. Despite the fact, that the majority of known hypermorphic mutations which cause extensive virus-induced cell fusion occur within glycoprotein K (gK); yet the role …


The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan Jan 2012

The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan

LSU Master's Theses

The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and DNA structure when present during cryopreservation of equine spermatozoa,. We hypothesized that the addition of iodixanol would improve post-thaw values for measured parameters. Ejaculates from six stallions were collected, centrifuged at 900 x g for ten minutes to remove supernatant, and suspended to 200 x 106 cells/ml with 0%, 2.5% and 5% iodixanol in an egg-yolk based extender and cryopreserved. Before and after cryopreservation sperm motility was assessed by computer assisted semen analysis, and samples …


Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto Jan 2012

Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto

LSU Master's Theses

Laminitis is an often fatal condition in horses with few available and only moderately effective treatment options. Separation of laminar dermis and epidermis lead to rotation or ventral deviation of the third phalanx inside the hoof capsule. Despite being a modified skin, equine laminar tissue does not completely return to normal after laminitis. The hypothesis tested was that adult progenitor cells in the equine laminar tissue are irreversibly damaged by laminitis. A method to harvest and culture cells in vitro from the equine lamina was established; and progenitor cells from unaffected and laminitic hooves were characterized and compared. Laminar tissue …


Essential Role Of Monocyte Chemoattractant Protein-1 In Gram-Negative Bacterial Pneumonia, Gayathriy Balamayooran Jan 2012

Essential Role Of Monocyte Chemoattractant Protein-1 In Gram-Negative Bacterial Pneumonia, Gayathriy Balamayooran

LSU Doctoral Dissertations

Acute gram-negative bacterial infections are a leading cause of mortality among the nosocomial infections. Increasing numbers of immunosuppressed individuals and growing numbers of antibiotic resistant strains make antibiotic treatment difficult. Neutrophils are the first cells recruited to the site of infection and are critical players in the host defense against gram-negative bacterial pneumonia. Therefore, identification of targets that boost neutrophil-associated host defense in the lung is essential in designing better therapies to control pulmonary infections. Production of chemokines is an important step for neutrophil recruitment. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that is important for monocyte and T-lymphocyte influx. …


Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham Jan 2012

Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham

LSU Master's Theses

ABSTRACT Objectives - To compare the osteointegration of plasma sprayed hydroxyapatite (HA) coated and uncoated 5.5 mm cortical screws in equine third metacarpal (MC3) bones in combination with a 4.5mm broad dynamic compression plate (DCP). Study design - In vivo study. Animals – 6 Adult Thoroughbred horses. Methods – For each horse, four HA coated screws were placed unicortically through a 4.5mm broad DCP in the dorsal cortex of a randomly chosen MC3 bone, with 4 uncoated screws placed in an identical manner in the contralateral MC3 bone. All screws were tightened to a torque of 5.4 N-m. Extraction torques …


Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu Jan 2012

Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu

LSU Master's Theses

West Nile virus (WNV) is one of several flaviruses known to infect mammalian species, including humans. There were 15,257 horse cases reported in 2002 and 1,086 in 2006 in United States. Recently, significant increases in equine and human cases have been reported in United States. Domain III of the WNV envelope protein binds to cellular receptors, and induces a significant portion of the neutralizing antibody response against the virus. CD40 Ligand (CD40L, CD154) enhances productive interactions between T cells and APC and has been shown to function as a potential adjuvant. In this study, we constructed and expressed a fusion …


The Role Of Gylcoprotein K (Gk) In The Ocular And Neuropathogenesis Of Herpes Simplex Virus-Type 1 (Hsv-1), Andrew Timothy David Jan 2012

The Role Of Gylcoprotein K (Gk) In The Ocular And Neuropathogenesis Of Herpes Simplex Virus-Type 1 (Hsv-1), Andrew Timothy David

LSU Doctoral Dissertations

Herpes simplex virus type 1 (HSV-1) is a DNA virus that is a ubiquitous pathogen of humans. The hallmark of the HSV-lifecycle is infection of a mucosal surface with spread to sensory neurons where the virus establishes a latent infection with periodic recurrences for the life of the host. The most common course of symptomatic disease with HSV-1 is the typical mucocutaneous lesion that is self-limiting. HSV-1 can also cause acute encephalitis and ocular pathology on reactivation. Both of these manifestations of the disease have severe consequences. Although ocular infection is less frequent, the extensive prevalence of HSV makes it …


Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis, Britton Grasperge Jan 2012

Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis, Britton Grasperge

LSU Doctoral Dissertations

Rickettsia parkeri, a member of the spotted fever group of Rickettsia, is the agent of an emerging rickettsiosis in the southeastern United States and South America. Despite increased recognition of human cases, limited information is available regarding infection of invertebrate and vertebrate hosts for this emerging tick-borne disease. Towards development of a viable transmission model and to further characterize the pathology associated with R. parkeri infection, inbred mouse strains (A/J, Balb/C, C3H/HeJ, and C3H/HeN) were intravenously and intradermally inoculated with R. parkeri. The C3H/HeJ strain of mice were identified as the most susceptible to R. parkeri infection and were found …


A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Sinan Ali, Culum Brown Dec 2011

A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Sinan Ali, Culum Brown

Aquaculture Collection

The stress response of wild and captive reared rainbowfish (Melanoteania duboulayi) following chasing by a simulated predator was examined. Cortisol release rate was monitored using a flow through system by measuring water borne hormone levels. Tests using known cortisol concentrations revealed that the technique yielded 95% of the cortisol present in the water. Cortisol release rates increased several fold in both populations after being chased but peaked at different time periods. Wild fish showed a typical stress response with release rate rising to (2.29±0.22 ng g−1 h−1) 2 h after exposure followed by rapid recovery. The captive-reared …