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Articles 4591 - 4620 of 9758

Full-Text Articles in Veterinary Medicine

Evaluation Of Ovsynch Protocols For Timed Artificial Insemination In Water Buffaloes In Bangladesh, Md Nazmul Hoque, Anup Kumar Talukder, Masuma Akter, Mohammed Shamsuddin Jan 2014

Evaluation Of Ovsynch Protocols For Timed Artificial Insemination In Water Buffaloes In Bangladesh, Md Nazmul Hoque, Anup Kumar Talukder, Masuma Akter, Mohammed Shamsuddin

Turkish Journal of Veterinary & Animal Sciences

A total of 65 water buffaloes (groups A, B, and C) at =>60 days postpartum with a body condition score (BCS) of =>2.5 were selected to evaluate ovsynch protocols for timed artificial insemination (TAI). The group A buffaloes (n = 25) were treated with a simple ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 2 - GnRH -16 h - TAI). The group B buffaloes (n = 22) received PGF2 alpha treatment 12 days before the initiation of simple ovsynch (PGF2 alpha at Day -12 + simple ovsynch; modified ovsynch). The group C buffaloes (n …

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Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva Jan 2014

Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to assess changes in acute phase proteins fibrinogen and haptoglobin, as well as hematological, blood biochemical, and microbiological alterations in local Bulgarian goats with experimentally induced Staphylococcus aureus mastitis. The experiments were conducted with 6 clinically healthy local Bulgarian goats, 2 to 6 years of age, weighing 46-57 kg. The experimental infection was done with a stationary phase culture of a field Staphylococcus aureus strain isolated from a cow with clinical mastitis. Blood haptoglobin in goats was considerably higher (P < 0.05) as early as 8 h after the pathogen's inoculation. The most significant differences from baseline values occurred 24 and 48 h (P < 0.01) after infection. Plasma fibrinogen increased significantly (P < 0.05) 8 h after infection and reached the highest mean concentration (9.12 g/L) by 72 h. Leukocytosis was established as early as 4 h after the experimental infection when leukocyte counts were significantly higher than baseline (P < 0.001). The analysis of results indicates that in experimentally induced Staphylococcus aureus mastitis, significantly higher (P < 0.05) levels of the leukocyte, fibrinogen, and haptoglobin are established as early as 8 h after the infection.

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The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek Jan 2014

The Effects Of Lh On Progesterone Production By Cell Subpopulations Isolated From Early And Late Luteal Phase Goat Corpora Lutea, Hakan Kalender, Şevket Arikan, Özkan Şi̇mşek

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the complementary effects of luteinizing hormone (LH) on cell subpopulations. Corpora lutea were collected on days 5 and 15 of the cycle. The luteal tissues were treated with collagenase for cell dissociation. Percoll density-gradient centrifugation was performed to separate cells into 2 distinct subpopulations. Luteal cells (5 × 104 cells/well) were cultured with and without 22(R)-hydroxycholesterol (22R-HC, 10 µg/mL) and LH (10 and 100 ng/mL) alone, and also with 22R-HC (10 µg/mL) plus LH (10 and 100 ng/mL), in a serum-free culture medium for 6 days of incubation. Untreated cells isolated from …

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Proximate Composition And Mineral And Fatty Acid Profiles Of Male And Female Jinga Shrimps (Metapenaeus Affinis, H. Milne Edwards, 1837), Mehmet Tolga Di̇nçer, İlker Aydin Jan 2014

Proximate Composition And Mineral And Fatty Acid Profiles Of Male And Female Jinga Shrimps (Metapenaeus Affinis, H. Milne Edwards, 1837), Mehmet Tolga Di̇nçer, İlker Aydin

Turkish Journal of Veterinary & Animal Sciences

The jinga shrimp (Metapenaeus affinis, H. Milne Edwards, 1837) is a commercially valuable alien decapod and a seafood product that is highly popular at seafood restaurants in İzmir (Turkey). The chemical, mineral, and fatty acid compositions of male and female jinga shrimps harvested in the Mediterranean Sea were determined for the first time. The compositions of both sexes confirmed that the jinga shrimp is a healthy food source due to its balance of nutrients, with efficient levels of protein and mineral contents. In fatty acid composition, the saturated fatty acid fraction was dominant, followed by polyunsaturated fatty acid and monounsaturated …

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Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report, Marudhai Thangapandiyan, Ramasamy Sridhar, Kirubakaran Jeyaraja, Masilamani Sakthivelan, Thangaraj Mohanapriya, Natesan Pazhanivel Jan 2014

Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report, Marudhai Thangapandiyan, Ramasamy Sridhar, Kirubakaran Jeyaraja, Masilamani Sakthivelan, Thangaraj Mohanapriya, Natesan Pazhanivel

Turkish Journal of Veterinary & Animal Sciences

A 9-year old male, German shepherd dog was presented to the Madras Veterinary College Teaching Hospital with a history of a chronic ailment that had increased over the previous few months. A diagnosis of chronic renal failure and hepatitis was made and abdominal ultrasonography revealed the presence of gallbladder mucocele (GBM). The etiology, diagnosis, necropsy, and histopathological lesions were discussed.

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A Detailed Evaluation Of The Skeletal Elements Of The Skull In The Grey Heron (Ardea Cinerea), Şükrü Hakan Atalgin, Emi̇ne Ümran Bozkurt Büyükçopur, İbrahi̇m Kürtül Jan 2014

A Detailed Evaluation Of The Skeletal Elements Of The Skull In The Grey Heron (Ardea Cinerea), Şükrü Hakan Atalgin, Emi̇ne Ümran Bozkurt Büyükçopur, İbrahi̇m Kürtül

Turkish Journal of Veterinary & Animal Sciences

This study was designed to reveal, in detail, the features of the skull bones in the grey heron, a species of the family Ardeidae, and to compare the findings with previous related literature reports and with Nomina Anatomica Avium. The articulation between the nasal and frontal bones was observed to be through the movable frontonasal joint. The upper beak fused with the neurocranium via this joint and with the palatine and vomer. It was also bound indirectly through the zygomatic bone articulating with the quadrate bone. The frontal bone possessed the frontal depression dorsally. Wideness of the frontal bone and …

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Correlations Among Oocyte Quality, Hepatic Triacylglycerols, And Some Blood Metabolites In Carora Breed Cows During Early Postpartum, Leandro Rodriguez, Oswaldo Rosendo, Carlos Parraga, Armando Oropeza Jan 2014

Correlations Among Oocyte Quality, Hepatic Triacylglycerols, And Some Blood Metabolites In Carora Breed Cows During Early Postpartum, Leandro Rodriguez, Oswaldo Rosendo, Carlos Parraga, Armando Oropeza

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the relationships of hepatic triacylglycerol (TAG), plasma glucose, plasma cholesterol, and plasma urea nitrogen (PUN) concentrations with the morphological quality of oocytes obtained from 20 Carora breed lactating cows at days 20 and 35 postpartum in a commercial farm. Oocytes were obtained through the technique of transvaginal ultrasound-guided follicle aspiration. Change in body condition score (0.35 vs. 0.44, P = 0.02) and mean plasma cholesterol (3.59 vs. 4.35 mmol/L, P = 0.01) significantly differed between the 2 periods, whereas mean TAG tended to be higher at day 35 after calving (2.29 vs. …

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Meat Quality Characteristics In Kıvırcık Lambs, Engi̇n Yarali, Onur Yilmaz, İbrahi̇m Cemal, Orhan Karaca, Turgay Taşkin Jan 2014

Meat Quality Characteristics In Kıvırcık Lambs, Engi̇n Yarali, Onur Yilmaz, İbrahi̇m Cemal, Orhan Karaca, Turgay Taşkin

Turkish Journal of Veterinary & Animal Sciences

Eşme District in Uşak Province has a special role in western Anatolia sheep husbandry due to its number of animals and large pastures. A genotype with the characteristics of the Kıvırcık breed has emerged and became common in the region in the last 20-30 years. This study aimed to determine the meat quality characteristics of Kıvrıcık lambs reared in the locality, which has a large market share. The study determined parameters about water and cooking loss and shear force in M. longissimus dorsi, M. longissimus thoracis, and M. semitendinosus muscles taken from the left half of lamb carcasses. Furthermore, meat …

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The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao Jan 2014

The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao

Turkish Journal of Veterinary & Animal Sciences

Recent progress in genomic analysis and other biochemical methods has led to the discovery of a large population of microRNAs (miRNAs), which have been demonstrated to play important roles in diseases and a wide range of developmental processes. Characterization of miRNA expression profiles in different stages of ovarian follicular development and early embryogenesis has suggested the potential roles of miRNAs in follicular development, maturation of oocytes, and preimplantation embryonic development. This review focuses on the current studies of miRNAs involved in ovarian and early embryonic development in cattle.

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Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa Jan 2014

Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa

Turkish Journal of Veterinary & Animal Sciences

Goat genetic diversity studies are very important since extinction of germ plasma is increasing very rapidly. The African and Asian continents are the hotspots for indigenous animals with fewer genetic manipulations. Conservation studies using molecular markers have the capability to validate the real status of the animals. The use of molecular markers has revolutionized studies of genetic diversity. Even though a number of markers are used for these types of studies, short sequence repeats are in the forefront due to their superior features such as high variability, high mutation rate, large number, distribution throughout the genome, codominant inheritance, and neutrality …

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Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos Jan 2014

Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos

Turkish Journal of Veterinary & Animal Sciences

Uterine diseases are highly prevalent in dairy cows. Causes of uterine diseases are multifactorial. There is good evidence for the susceptibility of the host and for the role of pathogenic bacteria, and less evidence for the effect of the environment. Uterine and leukocyte immune response is impaired early postpartum in cows that develop uterine disease. The decrease in immune function is associated with a decrease in calcium postpartum and an increase in NEFA and BHBA. Both endometrial cells and granulosal cells possess toll-like receptors (TLR) that can recognize and mount an inflammatory response to pathogen-associated molecular patterns (PAMPs) such as …

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Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs Jan 2014

Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs

Turkish Journal of Veterinary & Animal Sciences

Proper embryonic development depends upon regulatory signals from the reproductive tract called embryokines. This review uses the cow as a model to understand the developmental processes controlled by embryokines. The focus is on 3 embryokines that have been shown to increase competence of embryos to survive after transfer to recipients: CSF2, IGF1, and DKK1. Together, these molecules regulate key events required for development of the embryo to the blastocyst stage including proliferation (enhanced by IGF1), development of the inner cell mass (regulated by CSF2), control of differentiation (modified by DKK1), and inhibition of apoptosis and stress-mediated developmental arrest (IGF1 and …

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Application Of Dietary Fish Oil In Dairy Cow Reproduction, Anna Zwyrzykowska, Robert Kupczynski Jan 2014

Application Of Dietary Fish Oil In Dairy Cow Reproduction, Anna Zwyrzykowska, Robert Kupczynski

Turkish Journal of Veterinary & Animal Sciences

Feed fats are commonly used as a component of high-yielding cow diet rations. Many studies have been conducted on the effects of fat supplementation on metabolism, milk composition, and improvements in dairy cow reproduction. The positive effects on fat (vegetable oil) and energy supplementation on improvements in reproduction are well documented. Consumption of polyunsaturated fatty acids (PUFAs), including n-3 acids, in appropriate feed doses improves fertility of dairy cows. A fat source with high levels of acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is marine oil, especially fish and algae. By modification of cow rations, EPA and …

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Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor Jan 2014

Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor

Turkish Journal of Veterinary & Animal Sciences

Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first is a trait exhibiting complete dominance, whereas the latter is recessive. The extreme case of intersexualism is complete sex reversal, which is observed only in females (60,XX). The trait PIS is inherited in a recessive manner and caused by deletion of 11.7 kbp DNA on chromosome 1q43. Female goats with PIS exhibit a broad range of developmental abnormalities of the sexual system, from only subtle changes to complete sex reversal. The phenotypic diversity in goats with PIS is most …

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Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇ Jan 2014

Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇

Turkish Journal of Veterinary & Animal Sciences

Bull fertility, defined as the ability of the spermatozoon to fertilize and activate the ovum and then support embryonic and even fetal development, is a crucial factor influencing animal reproduction and production efficiency. Despite significant influences of fertility on herd genetic improvement and efficient cattle production, the mechanisms regulating this phenotype and reliable biomarkers associated with bull fertility are poorly defined. Specifically, there is a broad lack of knowledge in bull fertility. In this paper, bull fertility is reviewed within the context of animal systems physiology, and system biology markers associated with bull fertility are provided. This mini-review is intended …

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Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky Jan 2014

Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky

Turkish Journal of Veterinary & Animal Sciences

Improving sire reproductive performance in artificial insemination (AI) programs and maximizing the use of sires with valuable genomes are high on the list of priorities for livestock breeders in the United States and worldwide. While fixed-time AI and accurate estrous detection have already ameliorated the reproductive management of cows and sows, substantial gains remain to be made in the selection and utilization of male animals for field AI. In addition to better, more objective evaluation of breeding soundness and semen quality, numbers of spermatozoa per AI dose could be reduced and numbers of AI doses per collection could be increased …

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Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh Jan 2014

Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh

Turkish Journal of Veterinary & Animal Sciences

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (

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Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy Jan 2014

Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy

Turkish Journal of Veterinary & Animal Sciences

Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides …

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The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson Jan 2014

The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson

Turkish Journal of Veterinary & Animal Sciences

This review focuses on recent research related to the avian ovary and, in particular, cellular mechanisms mediating embryonic growth of the ovary, postembryonic follicular development, and the process of follicular selection. Attention is drawn to a variety of fundamental, yet unresolved, aspects of ovarian function, several of which are uniquely related to avian reproductive strategies. Some notable differences in ovarian function between birds and the most closely related extant group, the crocodilians, are illustrated. Moreover, because the sauropsid (reptilian and avian) and synapsid (mammalian) lineages diverged shortly after the emergence of amniotes some 340 million years ago, consideration is given …

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Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy Jan 2014

Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy

Turkish Journal of Veterinary & Animal Sciences

This research was conducted to study hormonal and metabolic statuses and to determine ultrasound criteria for early diagnosis of fetal growth retardation syndrome (FGRS) in dairy cows of Black-motley (n = 41) and Red-motley (n = 56) Holstein breeds. Concentrations of progesterone, testosterone, 17beta-estradiol, dehydroepiandrosterone sulfate, cortisol, and triiodothyronine in blood serum were assessed by immune-enzyme analysis. Concentrations of plasma L-ascorbic acid and nitrogen oxides, serum immune globulin, middle molecular peptides, and bactericidal and lysozyme activity were determined by spectrophotometry. On days 38-40 and 60-65 of gestation, FGRS in cows was associated with hypoprogesteronemia, decrease of adrenal and thyroid gland …

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Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova Jan 2014

Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effect of inbreeding on the yield and quality of bovine embryos. The data were obtained from 125 Holstein cows superovulated on 3 farms in the Czech Republic. In total, 125 flushings of embryos were analyzed, from which 761 embryos were obtained. Of all donors, 36 cows were inbred (199 embryos) and 89 cows were noninbred (562 embryos). The inbred cows were divided into 2 groups according to their inbreeding coefficient (FX) level. The low FX group (FX 1.26%-1.56%) had 23 cows and 147 embryos, and the high FX group (FX 3.1%-25%) …

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The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova Jan 2014

The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova

Turkish Journal of Veterinary & Animal Sciences

The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups (n = 7 for each) in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats (500 g per head per day). Both groups were treated intramuscularly with 12.5 µg/head lecirelinum (luteinising hormone releasing hormone super analogue) on the first day of supplementation …

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Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh Jan 2014

Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh

Turkish Journal of Veterinary & Animal Sciences

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 …

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Survivability Of Caprine Oocytes Vitrified In Conventional And Open Pulled Straws, Rameez Ali, Waquar Razzaque, Utsav Sharma, Anil Pandey, Kafil Hussain Jan 2014

Survivability Of Caprine Oocytes Vitrified In Conventional And Open Pulled Straws, Rameez Ali, Waquar Razzaque, Utsav Sharma, Anil Pandey, Kafil Hussain

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the efficacy of conventional straw vitrification with open pulled straw vitrification in terms of cryosurvivability and damages caused to the oocytes. Two hundred immature goat cumulus oocyte complexes (COCs) were vitrified in a solution of ethylene glycol, dimethyl sulfoxide, and sucrose using either conventional straws (100 COCs) or open pulled straws (100 COCs). The COCs were warmed and observed for morphological damages and viability after 7 days of preservation in liquid nitrogen. Among the 100 COCs cryopreserved in each case, only 83 COCs were recovered after warming in conventional straw vitrification as …

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Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek Jan 2014

Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of the use of Theranekron alone or in combination with intramammary antibiotic therapy for the treatment of subclinical and clinical mastitis on the recovery rate from mastitis. The study material was composed of 177 mammary lobes of 63 Holstein cows with subclinical (groups S1 and S2) and clinical mastitis (groups C1, C2, C3, and C4). In all of the treatment groups, excluding group S2, the California mastitis test scores were determined to have decreased after treatment (P < 0.05). In groups C1, C2, C3, and C4, which were treated for clinical mastitis, the somatic cell count (SCC) values were observed to have decreased. The decrease in SCC values was statistically significant except in group C2 (P < 0.01). While the SCC values were observed to have decreased in group S1 (P > 0.05), which was treated with Theranekron alone, the same values were found to …

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New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin Jan 2014

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …

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Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats, Chainarong Navanukraw, Jiratti Thammasiri, Tossapol Moonmanee, Jaruk Nutthakornkul Jan 2014

Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats, Chainarong Navanukraw, Jiratti Thammasiri, Tossapol Moonmanee, Jaruk Nutthakornkul

Turkish Journal of Veterinary & Animal Sciences

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

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The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem Jan 2014

The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem

Turkish Journal of Veterinary & Animal Sciences

Different levels of pomegranate peel extracted by either water (PEH) or solvent mixture (PEM) were evaluated for their effect on in vitro gas production and ruminal fermentation patterns. Two levels of PEH or PEM (15 and 30 mg total phenolics (TP)/g DM of basal diet) were compared to a control (0) level. Ruminal fermentation parameters such as 24-h partitioning factor (PF_{24}), gas yield (GY_{24}), in vitro organic matter disappearance (IVOMD), metabolizable energy (ME), and microbial protein (MP) were calculated. The addition of PEH or PEM had no effect (P > 0.05) on asymptotic gas production, apparent degraded substrate (ADS), IVOMD, ME, …

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Polymorphism Of The Ovine Calpastatin Gene In Some Turkish Sheep Breeds, Onur Yilmaz, Tamer Sezenler, Nezi̇h Ata, Yalçin Yaman, İbrahi̇m Cemal, Orhan Karaca Jan 2014

Polymorphism Of The Ovine Calpastatin Gene In Some Turkish Sheep Breeds, Onur Yilmaz, Tamer Sezenler, Nezi̇h Ata, Yalçin Yaman, İbrahi̇m Cemal, Orhan Karaca

Turkish Journal of Veterinary & Animal Sciences

The calpastatin (CAST) gene has a major effect on muscle growth and meat tenderness after slaughter; it is located on the fifth chromosome in sheep. Blood samples were collected from 720 animals in total from Kıvırcık (KIV), Sakız (SZ), Karacabey Merino (KM), and Gökçeada (GA) sheep populations raised in West Anatolia. The PCR products were digested by the restriction endonuclease MspI. Allele frequencies for M and N alleles of the gene were found to be 0.85 and 0.15 in KIV, 0.80 and 0.20 in KM, 0.99 and 0.01 in GA, and 0.34 and 0.66 in SZ sheep, respectively. It was …

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Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm Jan 2014

Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm

Turkish Journal of Veterinary & Animal Sciences

Three-\beta-hydroxysteroid dehydrogenase (3\beta-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3\beta-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + …

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